Digital Commons Network^™

Cho-K1 Host Cell Engineering Strategy Enabling The Establishment Of Strains Producing Higher Yields Of Recycling Antibodies, Hisahiro Tabuchi, Ikumi Murakami, Masaki Yamaguchi

Cell Culture Engineering XV

As described elsewhere (Biotechnol Bioeng 2010, 2013), our DXB11 (dhfr^–) host cell engineering strategy achieved high cell viability for a prolonged period (more than 1 month) and enhanced mAb productivity (>100 pg/cell/day) by nutritional control. Introduction of taurine transporter (TAUT) into DXB11 parent cells increased glutamine uptake and accelerated glutathione metabolism. By forcing the overexpression of TAUT, we were able to control DXB11 host cell functions and thereby increase the monoclonal antibody (mAb) titer up to 8.1 g/L/31 days under conventional 1-L bioreactor fed-batch conditions. Furthermore, the mAb produced by the DXB11/TAUT cells was comparable in quality …

Isolating Factors That Influence Grazing Patterns In Dairy Cattle, Julia N. Barney Mrs.

Honors Theses

Factors influencing grazing patterns were identified utilizing remote sensors on lactating Holstein and Brown Swiss dairy cattle in order to recommend efficient management techniques to dairy farmers. Over the course of three separate two week periods- one in the spring, summer and fall- data was collected on the dairy herd at Eastern Kentucky University’s Meadowbrook Farm. Approximately 18 cows were used each trial period; half being Brown Swiss and the other half being Holstein. Data was collected via CowManager ear tags, which tracked the five behavioral patterns of eating, ruminating, high, medium, and no activity. These activities served as dependent …

Teachers Gather For Second Annual Stem Educators Summit, Kyle Belmont

UNH Today Archive

No abstract provided.

Nonprofit Financial Assessment And Research Service Learning: Evaluating The Performance Of An Animal Welfare Nonprofit Organization, Karen A. Maguire

Accounting

The objective of this project is to evaluate the current financial and compliance status of an animal welfare nonprofit organization (NPO) by: analysis of trends over time using information reported on tax filings (Form 990); vertical and horizontal analyses of financial statements; analysis of trends over time using information from financial statements; reconciliation of financial statements to Form 990; ratio analysis of Form 990 Information; and comparison of reported information to local analogs and national standards. This project is conducted in collaboration with The Chapin Foundation. This research serves as a research service learning project with the participation of Master …

Comparison Of Two Body Weight Screenings Of Self-Conscious Emotions And Coping, Rachel N. Nanez Ms.

Electronic Theses and Dissertations

Novel technologies are currently being utilized in fitness and clinical settings. However, there is not much research to examine the emotional responses to such screenings.

PURPOSE: To examine the group differences between the FIT3D and DXA protocol on emotional responses and the differences between weight classifications.

METHODS: The Body and Appearance-related Self-conscious Emotions Scale and WEIGHTCOPE were used to assess self-conscious emotions and intention to utilize 10 different weight-related coping strategies following the scans. Motivation was measured by a 10-point Likert scale measuring motivation pre and post scans.

RESULTS: There were no differences in motivation, affective response, or intention to …

Development Of A System Model For An Indirect Passive Solar Dryer With Experimental Validation, Samantha Huselstein

Theses

Solar crop drying is a cheap and effective way to preserve food material, especially in developing countries where fuel and electricity are expensive or unavailable. Some tropical fruits are difficult to transport and store leading to significant spoilage. Without access to fuel and large drying systems, preserving fruits for later use is challenging or not possible for the rural farmer. Developing low-cost, easily assembled locally and low maintenance fruit drying systems would improve access to off-season and distant markets. A mathematical model of an indirect passive solar drying system was developed to design and optimize drying systems for use in …

Stop The Pricks: A Validation Study Of A Noninvasive Lactate Threshold Device, Rebecca M. Mcmorries

Electronic Theses and Dissertations

ABSTRACT

Purpose: The aim of this study is to assess the validity of a noninvasive lactate threshold device (NID) to determine lactate threshold heart rate (LTHR) and aerobic threshold heart rate (AeTHR).

Methods: Twenty-one recreational athletes completed a personalized graded exercise test on a treadmill. All participants wore the noninvasive device and blood lactate samples were taken at the end of 3-minute stages. Lactate threshold heart rate and aerobic threshold heart rate were then calculated using four traditional methods and compared against the same heart rate values calculated by the device.

Results: No significant differences were found in lactate threshold …

The Murray Ledger And Times, May 9, 2016, The Murray Ledger And Times

The Murray Ledger & Times

No abstract provided.

Lingnan Gardeners Newsletter (No. 18) = 嶺南彩園通訊 (第18期), Lingnan Gardeners, Kwan Fong Cultural Research And Development Programme, Lingnan University

Lingnan Gardeners Newsletter 嶺南彩園通訊

Grain Buds 小滿

South Dakota State University Graduate Commencement, May 7, 2016, Brookings, South Dakota, South Dakota State University

Commencement Programs

Please be advised that the names listed in the program do not constitute official confirmation of meeting the graduation requirements. The South Dakota State University 2016 Graduate Commencement ceremony will commence with introductions by University President David L. Chicoine. Honorary marshals are Ruth Harper, Professor of Counseling and Human Development, and RL Erion, Professor of Teaching, Learning, and Leadership. Delivering the commencement address titled "#YourFutureHealth" is David Pearce, President of Sanford Research. Giving comments on behalf of the graduating class is Valerie Kleinjan, a candidate for graduation.

South Dakota State University One Hundred-Thirtieth Undergraduate Commencement, May 7 , 2016, Brookings, South Dakota, South Dakota State University

Commencement Programs

Please be advised that the names listed in the program do not serve as official confirmation of meeting the graduation requirements. The 130th Undergraduate Commencement ceremonies at South Dakota State University are scheduled for May 7, 2016, featuring both morning and afternoon sessions. Each ceremony will begin with introductory remarks by University President David L. Chicoine. Provost Laurie Nichols, Vice President for Academic Affairs, will recognize student honors. Lt. Col. Craig McGuin and Lt. Col. Corey Ness will acknowledge Army and Air Force commissions. The MORNING ceremony will confer undergraduate degrees from the College of Agriculture, Food, and Environmental Science, …

Predicting Student Involvement In Campus Recreation Programs, Nicole Grabowski

Senior Honors Projects, 2010-2019

Purpose: To identify factors that predict the frequency of campus recreation (CR) use at a 4-year, public university in the mid-Atlantic region.

Methods: Students were given an online survey to collect a variety of student lifestyle and health information, including campus residency status, gender, year, height, weight, academic discipline, semester credit hour enrollment, and job hours per week during the semester. Analysis participants (n = 1561) were divided into two subsets, one with 90% of the subjects, and one with 10% of the subjects. A stepwise multiple regression analysis was performed on the 90% subset with the predictor variables. Two …

Macronutrient Supplementation For Endurance Athletes, Jonathan B. Hurst

Senior Honors Projects, 2010-2019

Purpose: The present study addressed two questions related to macronutrient supplementation during endurance exercise. Firstly, the effects of carbohydrate and protein co-ingestion on time trial (TT) performance were compared to carbohydrate alone. Secondly, the effects of isolated protein ingestion on TT performance were compared to a placebo.

Methods: Six trained cyclists (Age: 22 ± 1 years; Height: 167 ± 12 cm; Weight: 60 ± 10 kg; VO_2max: 62 ± 7 ml/kg/min) completed four experimental trials, consisting of constant-load cycling for two hours (55% W_max) immediately followed by a 30-km simulated time trial. During the trials, subjects …

Brain Derived Neurotrophic Factor Response During Endurance Cycle Training: Impact Of Carbohydrate And Protein Supplementation, Jordan L. Parker

Senior Honors Projects, 2010-2019

Purpose: Brain Derived Neurotrophic Factor (BDNF) is part of the superfamily of neurotrophins including: nerve growth factor (NGF), neurotrophin-3 (NT-3) and neurotrophin-4 (NT-4). Recent evidence suggests that aerobic activity can increase BDNF levels both during and following exercise. Likewise, dietary habits have been shown to alter BDNF levels. However, nothing is known about the effect of BDNF levels during aerobic activity under specific dietary interventions. The purpose of this study was to examine the effects of specific macronutrient supplementation on BDNF levels during aerobic exercise. Methods: We had the opportunity to determine BDNF levels in blood samples obtained from two …

Selected Health Behaviors Among Undergraduate College Students In Different Academic Disciplines, Patrick C. Gathman

Senior Honors Projects, 2010-2019

Physical activity, campus recreation (CR) use, body mass index (BMI), and varied health indices were compared between academic discipline (AD) groups and sex. Participants (n = 219) were classified as AD I (kinesiology and physical education majors), AD II (health science majors and nursing majors), and AD III (representative sample of other non-health-related majors) in order to make between group comparisons based on the amount of emphasis placed on physical activity and health-related content within different disciplines. Significant differences (p < 0.05) were found between the academic discipline groups for International Physical Activity Questionnaire (IPAQ) scores, CR minutes, CR days, CR time per day, vigorous physical activity (VPA), and perceived-health score; and between sex for BMI, VPA, sitting (SIT), fiber intake, and fruit and vegetable intake. When measuring CR use in total minutes per semester, days per semester, and minutes per day AD I was higher than AD II by 100%, 66%, and 21%, respectively; and AD I was higher than AD III by 247%, 160%, and 27%, respectively. The results indicate a positive relationship between the emphasis placed physical activity and health within an academic discipline and the degree to which students participate in physical activity, positive health behaviors and perceived health.

Analysis Of The Responses To Poverty In Harrisonburg, Virginia, Kara Krantz

Senior Honors Projects, 2010-2019

Lyndon B. Johnson’s War on Poverty was established in 1964 to eradicate poverty in the United States. There have been great strides towards accomplishing this goal; however, poverty continues to persist. The purpose of this study is to analyze poverty in Harrisonburg, Virginia and how it compares to poverty at the state and national levels. In addition, this study evaluates the services provided by nonprofits and government agencies to the poor in Harrisonburg. Finally, it investigates the interactions among the nonprofits and government agencies. Poverty statistics come from the U.S. Census Bureau. Analysis of the data reflects that the presence …

Assessment Of Contamination Levels And Estimation Of Dietary Intake Of Heavy Metals From Selected Imported Fruits And Vegetables In Ghana, Eric Dzimado

Theses and Dissertations

Levels and dietary intake of heavy metals were assessed and evaluated in thirty two samples of fruit and vegetables collected from supermarkets in Accra, Ghana using atomic absorption spectroscopy. Differences (p <= 0.05) existed in Pb, Cd and Ni concentrations across the commodities collected. Levels of Pb, Cd and Cr exceeded safe limits established by FAO and EU. Washing reduced Cr in cabbage by 59% (p <= 0.05) but only reduced Pb in grapes by 12% (p ˃ 0.05). Estimated daily and weekly intake through fruit and vegetable consumption were below provitional tolerable dietary intake established by the FAO. Although estimated dietary intake obtained in this study were below the maximum established limits, increased comsumption of these fruit and vegetables may bring about adverse health implications for the exposed population.

Exploration Of Malnutrition Coding Practices At Nebraska Medicine, Frances C. Becker

Theses & Dissertations

Background: Malnutrition is present in 25-50% of hospitalized patients. Patients identified as malnourished are assigned a code based on the type and severity of malnutrition in order to gain the necessary reimbursement to care for the patients. There is a current gap in the research regarding the characteristics that define the difference between malnourished patients classified with a major complications and comorbidities (MCC) code versus a complications and comorbidities (CC) code.

Purpose: The purpose of this study was to determine which malnutrition codes are being used at Nebraska Medicine. Additionally, this study obtained clinical factors that are associated with malnutrition …

Effects Of Prenatal Gestational Diabetes Nutrition Education Class And Individual Follow-Up On Maternal And Infant Outcomes, Samantha List

Theses & Dissertations

BACKGROUND: Gestational Diabetes Mellitus (GDM) is defined by glucose intolerance detected with the onset of pregnancy, and if gone undetected and untreated, can lead to morbidities for the mother and baby. Implementation of nutrition counseling can work to reduce the risk of complications through dietary modification promoted and adopted during pregnancy. Follow-up is important, as individual assessment is used to modify recommendations. GDM class and follow-up provided through the Diabetes Center of Nebraska Medicine covers and promotes proper management of blood glucose (BG) during pregnancy for women with GDM.

PURPOSE: The purpose of this study was to assess the impact …

Baseline Susceptibility Of Selected Lepidopteran Pests To Diamides And Use Strategies In Mississippi Soybean, Charles Andrew Adams

Theses and Dissertations

Insecticides in the diamide class have a novel mode of action and have become a key component for management of agriculturally important lepidopteran pests since their introduction in 2008. Corn earworm, Helicoverpa zea (Boddie); and the armyworm complex including fall armyworm, Spodoptera frugiperda (J.E. Smith); and Spodoptera exigua (Hübner); are significant pests of agroecosystems in the Mid-southern and Southeastern regions of the United States. They have developed resistance to, and/or inconsistent control has occurred with most chemical classes. The objectives of this study were to establish susceptibility levels of field populations of H. zea, S. frugiperda, and S. exigua collected …

Analyzing The Influence Oxygen Deprivation Has On The Capability Of Listeria Monocytogenes To Induce Listeriosis In Gerbils, Jillian Leigh Harris

Theses and Dissertations

Listeria monocytogenes is food-borne pathogen that causes listeriosis in individuals with a compromised immune system and pregnant women. This pathogen can survive in anaerobic conditions present in specially packaged foods as well as the gastrointestinal tract. The purpose of this study is to evaluate virulence of L. monocytogenes F2365 in anaerobic conditions. Another goal of this study is to establish gerbils as the ideal animal model since descrepancies exist in current models. Gerbils were orally infected with one of four doses: 1) phosphate buffered saline, 2) 5X106CFU aerobic dose, 3) 5X108CFU aerobic dose, and 4) 5X106CFU anaerobic dose. Results indicate …

An Exploration And Comparison Of Internalized Behaviors And Peer Relationships In Dual Enrolled And Non-Dual Enrolled Students, Mary Bess Woodard Pannel

Theses and Dissertations

While the importance of dual enrollment programs has been clearly demonstrated, the potential impact of completing college level courses during high school has on the emotional and mental well-being of adolescents has not been explored. School counselors are in a unique position to foster an academic environment that also enhances their emotional and mental wellness. Discovering factors that contribute to internalized behaviors, peer relationships, and academic performance, may provide school counselors a better understanding of personal, social, and academic development of adolescents The present study used a nonexperimental, comparative, research design to explore whether or not participation in dual-enrollment courses …

Diet Quality Of Adolescent Females In Relation To Disease Risk, Heidi Pfeifer

Theses & Dissertations

Background: Many people are living longer with chronic diseases costing billions in healthcare costs every year. Many things influence the risk of chronic diseases but one easily modified risk factor is diet. Alternate Healthy Eating Index (AHEI) and Alternate Healthy Eating Index-2010 (AHEI-2010) diet quality indexes have been associated with chronic disease risk but has not been studied in disease risk in adolescents.

Purpose: The purpose of this study is to look at adolescent girls’ diet quality scores compared with known adult values associated with chronic diseases and risk of mortality.

Methods: This is a secondary analysis of a previous …

Global Trends In Cancer Burden Based On Geographic Location, Socio-Economic Status And Demographic Shift, Sanjib Chowdhury

Theses & Dissertations

The global burden of cancer is rising at an alarming rate. It remains as one of the top causes of morbidity and mortality worldwide. It is predicted that within 2020, there will be 15 million new cases of cancer in the world, with cancer-related deaths increasing to 12 million. According to the World Health Organization (WHO), the global cancer burden is expected to surge 57% worldwide in the next 20 years. A significant rise in cancer burden will occur in the low-income and middle-income (developing) countries not only due to demographic shifts, but also by the transition of risk factors …

Proportion Of Heart Failure Patients Who Meet Criteria For Malnutrition Upon Hospital Admission Based On Aspen Guidelines, Sarah L. Johnson

Theses & Dissertations

Background: Current research estimates that approximately 50 percent of heart failure patients are categorized as malnourished. Heart failure patients are at increased risk of malnourishment due to increased catabolic processes that increase resting energy expenditure, decrease appetite, impair nutrient absorption, and lead to unintentional weight loss. The goal of standardized diagnostic criteria is to identify malnutrition early for more effective treatment. Current studies suggest the association of early diagnosis and nutrition intervention with increased positive patient outcomes and improved quality of life for heart failure (HF) patients. However, the proportion of patients with HF who meet criteria for malnutrition at …

Use Of The Heat Stress Score To Predict Preparedness To Run In An Outdoor, Warm Weather Race, Rachel K. Katch

Master's Theses

No abstract provided.

Vitamin A Compounds In Mothers And Infants At Birth, Jenna M. Paseka

Theses & Dissertations

OBJECTIVE: Little is known about the vitamin A status in mothers and infants at birth in the United States. The main objective of this study is to determine associations between maternal and infant serum retinol, provitamin A carotenoids, and non-provitamin A carotenoids. The secondary aim is to explore the relationship between maternal intake and maternal and infant serum levels of vitamin A compounds.

METHODS: This was a prospective cohort of 34 mothers and their infants admitted to the neonatal intensive care unit (NICU). Maternal and cord blood samples were collected at delivery. Serum retinol, lutein + zeaxanthin, β-cryptoxanthin, lycopene, α-carotene, …

Using The Minimum Data Set (Mds) To Triage Long Term Care Facilities’ Residents For A Systematic Evacuation In The Case Of Mass Casualty Disaster Events, Zoumana I. Traore

Theses & Dissertations

Long Term Care facilities preparedness for disasters has been questioned, and evacuation before or during disasters is crucial for life saving. This thesis attempted to develop a systematic triage methodology based on the use of the Minimum Data Set (MDS), which is a powerful resource for gathering residents’ vital information including physical ability, cognitive functioning, and other health related conditions. Our assumption was that 90% of caregivers’ triage categorization will match with the categorization generated from the MDS dataset. To achieve our objectives and aims, we compared the triage categorization carried out by residents’ caregivers versus the MDS generated triage …

Essays On Immigration-Related Disparities In Health Behavior And Health Care Utilization, Yang Wang

Theses & Dissertations

The number of immigrants in the United States has recently been increasing significantly. Immigrants may experience some worse health outcomes than natives, due to substantial legal and socioeconomic barriers. Many immigration-related disparities in health behavior and health care utilization still remain unexplored. This dissertation comprises of 3 independent studies examining such disparities across immigration status, including (1) E-cigarette use and acculturation effects; (2) Cancer-related office-based medical provider visits among cancer patients; and (3) Potentially preventable emergency department visits.

Two nationally representative data sources included National Health Interview Survey and Medical Expenditure Panel Survey. We categorized the respondents into three immigration …

Physical Activity Levels And Related Factors In Midwestern Rural Hispanic Or Latino Adults, Alison K. Clevette

Theses & Dissertations

The purpose of this dissertation was to examine physical activity levels and specific intrapersonal, interpersonal and community physical environment factors that influence physical activity in Midwestern rural Hispanic or Latino adults. Instruments used in the study included: Self-Efficacy for Exercise Behaviors Survey, Social Support for Exercise Survey, Short Acculturation Scale for Hispanics (SASH), and Neighborhood Environment Walkability Scale (NEWS). Physical activity was measured by 7-Day Physical Activity Recall and ActiGraph^® accelerometer data. In the study of 54 participants, it was found that there was a very low level of physical activity. For example, 97% of the time was spent …