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The Impact Of Mycobacterium Leprae: A Comprehensive Meta-Analysis Of The Paleopathological Literature, Mallory Alexis Schreier

Boise State University Theses and Dissertations

Leprosy, caused by the bacteria Mycobacterium leprae and Mycobacterium lepromatosis, is a chronic, infectious disease that eventually causes disfiguring skin lesions, nerve damage, and muscle weakness. Even though leprosy has been nearly eliminated in many parts of the world today, it remains endemic in India, Myanmar, Nepal, Brazil, and a few African countries. Unfortunately, this infectious disease is not limited to just modern populations. In the past, leprosy spread globally and was a pervasive, degenerating disease. The literature traces leprosy back to 1550 BCE although there is possible skeletal evidence of leprosy in Rajasthan, India from 2000 BCE, suggesting …

Reading Between The Lines: Indicators Of Developmental Stress In Prehistoric Ohio Valley Children From Linear Enamel Hypoplasias, Emily Moes

Boise State University Theses and Dissertations

A large body of research in bioarchaeology focuses on the changes in the human skeleton associated with the introduction of agriculture. It is assumed that the intensification of agriculture results in an increase in physiological stress and poor health. However, previous studies have shown that stress experiences cannot be generalized by subsistence strategy and prevalence comparisons alone. Rather, age-at and duration of stress events are necessary to construct patterns of health.

Linear enamel hypoplasias (LEH) of subadult permanent dentition serve as a proxy for understanding health and stress in archaeological populations. LEH are defects in enamel, characterized by an increased …

Investigation Of The Tailoring Steps In Pradimicin Biosynthesis, Kandy L. Napan

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This research focused on the investigation of the late steps in the biosynthetic pathway of the novel antifungal and antiviral pradimicins A-C. Pradimicins were first isolated from the soil bacterium Actinomadura hibisca. These bioactive molecules are assembled by a type II polyketide biosynthetic pathway. Although the biosynthetic gene cluster of pradimicin has been identified, the functions of the biosynthetic genes and how they work collaboratively to form the final structures of pradimicins remain unknown. This research aims to functionally characterize the enzymes involved in the late steps of the biosynthetic route.

The early biosynthetic steps of pradimicins have been …

Immunomodulation Of Breast Cancer Cells For Whole Tumor Vaccination, Kristina G. Maxwell

Biomedical Engineering Undergraduate Honors Theses

Hematogenous metastasis causes 90% of breast cancer-related deaths.Current therapies include chemotherapy and irradiation following surgery. These therapies are very harmful to the human body and do not elicit an anti-tumor immune response. To create a novel therapeutic, an autologous vaccine increasing the immunogenicity of non immunogenic breast cancer cell lines has been proposed.

To create this vaccine, 4T1 mouse mammary breast cancer cells have been selected as the desired cell line to treat. They are non immunogenic and highly invasive. In order to increase their immunogenicity, first projected, was the addition of cytokines to 4T1 cells to increase the expression …

Contribution Of Probiotics Streptococcus Salivarius Strains K12 And M18 To Oral Health In Humans: A Review, Turner A. Stowik

Honors Scholar Theses

The overgrowth and disequilibrium of pathogenic microorganism species both native and non-native to the oral cavity can manifest into a variety of different oral diseases, pathologies, and afflictions in humans, including dental caries, gingivitis, pharyngitis, halitosis, and oral candidiasis. Two bacterial strains with clinically-significant probiotic applications in curtailing the pathogenic bacterial growth involved in these conditions are Streptococcus salivarius strain K12 and Streptococcus salivarius strain M18. To summarize the most up-to-date in vitro, in vivo, and clinical research findings, administration of these S. salivarius strains typically in the form of probiotic lozenges results in colonization, reduction in inflammatory measures, …

Characterization Of Induced Rnai Silencing Of Vaccinia Virus Essential Genes, Kewa Jiang

Honors Scholar Theses

Vaccinia virus (VACV) is a large double-stranded DNA virus and the prototypical member of the family Poxviridae and is most notable for its use as the vaccine that eradicated smallpox (variola virus). More recently, VACV has been used to develop recombinant vaccines and immunotherapies. However, many of these processes require VACV replication to be tightly controlled. RNA interference (RNAi) is a powerful tool for in vitro silencing of mRNAs that are complementary to 19-21 base pairs (bp) of double-stranded RNA (dsRNA). This project outlines the design and preliminary analysis of two inducible RNAi silencing constructs targeting multiple VACV essential genes …

Sa-4-1bbl As A Modulator Of Innate, Adaptive, And Regulatory Immunity : Implications For Cancer Prevention And Treatment., Hampartsoum Barsoumian

Electronic Theses and Dissertations

SA-4-1BBL is a recombinant costimulatory molecule that is active in its soluble form and has pleiotropic effects on the functions of innate, adaptive, and regulatory immune cells. We have previously shown that SA-4-1BBL makes CD4+ T conventional cells (Tconvs) resistant to suppression by CD4+CD25+Foxp3+ T regulatory cells (Tregs). The mechanistic basis of this observation is the subject of this study. We demonstrate that Tconvs, but not Tregs, are the direct targets of SA-4-1BBL-mediated evasion of Treg suppression without contribution from antigen presenting cells (APCs). SA-4-1BBL engagement with its receptor, 4-1BB, on Tconvs resulted in the production of high levels of …

Wic Program Evaluation: A Breastfeeding Study, Christina Damouny

Master's Projects

Poor nutrition is prevalent among low-income pregnant and postpartum women, and infants. Inadequate nutrition during these vital stages can have negative effects on the health status of this population. The Women, Infants and Children Program (WIC) was established specifically to help low-income women and children lead healthy lives by providing nutrition education, referrals to healthcare and other health-related services, breastfeeding promotion, and economic resources to purchase nutritious foods at retail grocery stores. WIC has been shown to have an influence on participating mothers’ infant feeding decisions for both formula- feeding and breastfeeding. Although WIC is mandated to provide breastfeeding education …

Comparison Of Anti-Pneumococcal Functions Of Native And Modified Forms Of C-Reactive Protein, Donald Neba Ngwa

Electronic Theses and Dissertations

The anti-pneumococcal function of native C-reactive protein (CRP) involves its binding to phosphocholine molecules present on Streptococcus pneumoniae and subsequent activation of the complement system. However, when pneumococci recruit complement inhibitory protein factor H on their surface, they escape complement attack. Non-native forms of CRP have been shown to bind immobilized factor H. Accordingly, we hypothesized that modified CRP would bind to factor H on pneumococci, masking its complement inhibitory activity, allowing native CRP to exert its anti-pneumococcal function. As reported previously, native CRP protected mice from lethal pneumococcal infection when injected 30 minutes before infection but not when injected …

Tick-, Mosquito-, And Rodent-Borne Parasite Sampling Designs For The National Ecological Observatory Network [Special Feature: Neon Design], Yuri P. Springer, David Hoekman, Pieter T.J. Johnson, Paul A. Duffy, Rebecca A. Hufft, David T. Barnett, Brian F. Allan, Brian R. Amman, Christopher M. Barker, Roberto Barrera, Charles B. Beard, Lorenza Beati, Mike Begon, Mark S. Blackmore, William E. Bradshaw, Dustin Brisson, Charles H. Calisher, James E. Childs, Maria A. Diuk-Wasser, Richard J. Douglass, Rebecca J. Eisen, Desmond H. Foley, Janet E. Foley, Holly D. Gaff, Scott Lyell Gardner, Howard S. Ginsberg, Gregory E. Glass, Sarah A. Hamer, Mary H. Hayden, Brian Hjelle, Christina M. Holzapfel, Steven A. Juliano, Laura D. Kramer, Amy J. Kuenzi, Shannon L. Ladeau, Todd P. Livdahl, James N. Mills, Chester G. Moore, Serge Morand, Roger S. Nasci, Nicholas H. Ogden, Richard S. Ostfeld, Robert R. Parmenter, Joseph Piesman, William K. Reisen, Harry M. Savage, Daniel E. Sonenshine, Andrea Swet, Michael J. Yabsley

Scott L. Gardner Publications

Parasites and pathogens are increasingly recognized as significant drivers of ecological and evolutionary change in natural ecosystems. Concurrently, transmission of infectious agents among human, livestock, and wildlife populations represents a growing threat to veterinary and human health. In light of these trends and the scarcity of long-term time series data on infection rates among vectors and reservoirs, the National Ecological Observatory Network (NEON) will collect measurements and samples of a suite of tick-, mosquito-, and rodent-borne parasites through a continental-scale surveillance program. Here, we describe the sampling designs for these efforts, highlighting sampling priorities, field and analytical methods, and the …

An Insight Into The Molecular Characteristics Of Hepatitis C Virus For Clinicians, Lingyao Du, Hong Tang

Saudi Medical Journal

Hepatitis C virus (HCV) consists of envelope proteins, core proteins, and genome RNA. The structural genes and non-structural genes in the open reading frame of its genome encode functional proteins essential to viral life cycles, ranging from virus attachment to progeny virus secretion. After infection, the host cells suffer damage from virus-induced oxidative stress, steatosis, and activation of proto-oncogenes. Every process during the viral life cycle can be considered as targets for direct acting antivirals. However, protective immunity cannot be easily acquired for the volatility in HCV antigenic epitopes. Understanding its molecular characteristics, especially pathogenesis and targets the drugs act …

Plants, Prions And Possibilities: Current Understanding And Significance Of Prion Uptake Into Plants, Tracy A. Nichols

United States Department of Agriculture Wildlife Services: Staff Publications

Chronic wasting disease (CWD) is an infectious, neurodegenerative disease of deer (white-tailed and mule), elk, moose, sika deer and muntjac caused by a misfolded version of a normally occurring protein. The notion that CWD could be spread indirectly via the environment has been documented and accepted in the scientific community for quite some time. Deer and elk consume soil, inhale dust and lick objects that have infectious material on them, resulting in chronic, low dose exposure. Surface contamination of plants with urine or feces is likely an additional source of exposure via ingestion and has been modeled in the laboratory …

Ochratoxicosis In Monograstric Animals - A Review, Nafeesa Qudsia Hanif

Journal of Bioresource Management

Ochratoxin A (OTA) is a mycotoxin produced by several fungi of the genera Aspergillus and Penicillium, principally P. verrucosum in temperate climate and A. ochraceous in warm regions. In poultry feed materials, mycotoxins are found most commonly in cereals and to a lesser degree, in meals. The presence of OTA in animal feed contributes significantly to health disorders and decreased production. In addition to aflatoxins, which is an ubiquitously distributed toxin, OTA is one of the reasons for economic losses in the poultry industry due to poor performance and immunosuppression. Moreover, OTA has also been noted for the carryover …

Rerouting Pre-Existing Host Vaccine-Induced Immunity To Wards Breast Cancer, Bharat Kumar Reddy Chaganty

Dissertations and Theses (Open Access)

For decades, investigators have attempted to activate the immune system to prevent cancer metastasis or recurrence; however, owing to host immune tolerance to cancer antigens and the immunosuppressive environment at tumor sites, many such attempts have failed. The recent success of anti-CTLA4, PD-L1 and PD-1 antibodies targeting immune checkpoint pathways and HPV vaccines has renewed hope that patient survival can be increased through enhancing T-cell responses. We propose to test a novel approach that may bypass host immune tolerance to cancer cells. We hypothesize that host T-cell immunity acquired through vaccination against or natural infection with infectious diseases—e.g., influenza—can be …

Preeclampsia: The Roles Of Acute Inflammation And Intrauterine Stress, Nicholas Parchim

Dissertations and Theses (Open Access)

Preeclampsia (PE) is a severe, acute disease of pregnancy affecting approximately 8% of pregnant women after week 20 of gestation. PE is characterized by hypertension and renal damage reflected by proteinuria and has significant morbidity to both mother and fetus. Maternal symptoms range from headaches, nausea, edema, to visual changes, but once maternal symptoms present, damage to the fetus has begun. Mothers who progress untreated through the disease can also experience a condition called eclampsia characterized by seizure, coma, and, ultimately, death. PE-affected newborns experience features similar to prematurity—abnormal lung and renal development, intrauterine growth retardation (IUGR), and, possibly, fetal …

Assessment Of Microbial And Growth Response Of Broilers Fed A Commercial Prebiotic, Sang In Lee

Graduate Theses and Dissertations

Prebiotic refers to nondigestible food ingredients that enhance the health of the host by selectively promoting one or more number of beneficial bacteria. Gibson and Roberfroid (1995) initially described prebiotics as compounds that neither hydrolyzed nor absorbed in upper part of the gastrointestinal tract, selectively stimulates growth of beneficial bacteria, enhance health of the host and able to amend microorganism population to healthier states. Prebiotics are particularly attractive supplements in animal production due to their variety of effects including production of short chain fatty acids, pH amendments and suppression of pathogen colonization. In addition, prebiotics were emphasized as antibiotic alternatives …

Development Of A Simple Handheld Biosensor For Waterborne Pathogens, Bryce C. Jones

Chemical Engineering Undergraduate Honors Theses

Current methods of detecting waterborne pathogens involve testing strips which take 24-48 hours to yield results, or require expensive equipment in order to function. In minimalist environments, these two technologies are not always applicable to test water quality. With the emergence of a new method of PCR, named LAMP PCR, it is possible to quickly and accurately detect pathogen DNA in a water sample. In order to scale this technique into a simple device, the aspects of the reaction must be accommodated, and a visual detection method chosen. A handheld device which keeps the isothermal LAMP PCR stable for the …

Barriers To Health Care Access For Refugees In Cache County, Utah, Michael Timo Hoggard

Undergraduate Honors Capstone Projects

The United States has a rich history of resettling refugees. The United States resettled hundreds of thousands of refugees at the conclusion of World War II, and has resettled more than 3 million refugees since 1975 (Refugee Council USA, 2016). A refugee is a forcibly displaced person who will not or cannot return to their country of origin because of fear of physical harm or persecution (United Nations, 1951). Refugees are distinct from other forms of immigrants in many ways: refugees do not choose their country for resettlement, they receive government assistance upon arrival, their residency and work permits are …

Perceived Alzheimer's Disease Threat As A Predictor Of Behavior Change To Lower Disease Risk: The Gray Matters Study, Christine Clark

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Alzheimer’s disease is a growing public health concern with an estimated five million Americans currently afflicted. That number is projected to triple by 2050 as the baby boomer generation approaches age 65, the most common age where people begin to show symptoms of cognitive decline stemming from changes in the brain related to Alzheimer’s. Since there is currently no cure or preventive pharmacological treatment, AD prevention research is now recognized as an important enterprise. Modifiable lifestyle factors that can reduce AD risk or delay its onset have been identified as reasonable targets for behavioral intervention, including increased physical activity, healthier …

A Spatiotemporal Mountain Pine Beetle Outbreak Model Predicting Severity, Cycle Period, And Invasion Speed, Jacob P. Duncan

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The mountain pine beetle (MPB, Dendroctonus ponderosae), a tree-killing bark beetle, has historically been part of the normal disturbance regime in lodgepole pine (Pinus contorta) forests. In recent years, warm winters and summers have allowed MPB populations to achieve synchronous emergence and successful attacks, resulting in widespread population outbreaks and resultant tree mortality across western North America. We develop an age-structured forest demographic model that incorporates temperature-dependent MPB infestations: the Susceptible-Infested-Juvenile (SIJ) model. Stability of equilibria is analyzed as a function of population growth rates, and indicates the existence of periodic outbreaks that intensify as growth rates …

Synthesis, Structure-Activity Relationship Study, And Mode Of Action Study Of 1,4-Naphthoquinone Based Anticancer And Antimicrobial Agents, Jaya P. Shrestha

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The first three projects involve the synthesis and the mode of action study of 1,4-naphthoquinone based anticancer agents. These anticancer agents are highly potent against a wide range of cancer cell lines. These compounds showed ~8-fold selectivity towards the lung cancer cell line than the normal cell line. We also studied the mode of action and observed that generation of reactive oxygen species is the primary mode of action.

The final project involves the synthesis of multi-cationic antimicrobial agents. These compounds are active against both bacteria and fungi. These compounds are very easy to synthesize and can be scaled up …

Nutritional Deficiencies During The Harvest Season According To Household Consumption And Level Of Nutritional Knowledge: A Case Study Of Northern Mozambique, Maggie Jo Hansen

Graduate Theses and Dissertations

Mozambique is classified as a low income country, the lowest development classification defined by the World Bank. It is underdeveloped in food security, agricultural production, and nutritional status, and research shows its residents are not foreign to poverty and malnutrition. Low protein, starchy foods (maize, rice, wheat, cassava) comprise the majority of the Mozambican diet; these starchy foods are the most available for consumption. Although availability impacts diet, educational barriers may also threaten the knowledge of nutrition and perceptions of healthy foods. Illiteracy and lack of education are extreme challenges to disseminating nutritional education efforts in the rural Nampula region. …

Comparative Efficacy Of Foaming And Non-Foaming Handsoap In Reduction Of Microorganisms In Handwashing, Danielle Marie Conover

Graduate Theses and Dissertations

Handwashing (HW) is a long established method to prevent disease transmission. Ensuring effectiveness of current HW methods is essential for optimal HW and enhanced disease prevention. The objectives of this research were to 1) conduct a survey of soap type and volume in food service establishments in Washington County, Arkansas; 2) investigate how soap type impacts HW behavior; and 3) determine the difference in microbial reduction between foaming (F) and liquid (L) handsoap. For Objective 1, food service establishments in Washington County, AR were selected based on exclusion criteria and random number generations, and handsoap samples were collected to determine …

Evaluation Of Antibiotic Use In Pediatric Intensive Care Unit Of A Developing Country, Qalab Abbas, Anwar Ul Haq, Raman Kumar, Syed Asad Ali, Kashif Hussain, Sadia Shakoor

Department of Paediatrics and Child Health

Background: Pediatric Intensive Care Unit (PICU) patients are often prescribed antibiotics with a low threshold in comparison to patients elsewhere. Irrational antibiotics use can lead to rapid emergence of drug resistance, so surveillance of their use is important.
Objectives: To evaluate the use of antibiotics in relation to bacteriological findings in PICU of a Tertiary Hospital.
Methods: Retrospective review of medical records of all children (age 1 month-16 years) admitted in our closed multidisciplinary-cardiothoracic PICU from January to June 2013 was performed, after approval from Ethical Review Committee. For each antibiotic, indication (prophylactic, empiric, therapeutic) and duration of use were …

Proteomic Characterization Of Eggshell Membranes And Their Effect On Poultry Physiology And Immunity, Sarbjeet Kaur Makkar

Graduate Theses and Dissertations

The use of antibiotics in poultry growth and disease control has led to antibiotics resistant problem in human beings, which is a big concern among consumers. With the necessity for judicious use of antibiotics in poultry production, alternative strategies to improve disease resistance in poultry production are necessary. The research is more inclined towards using the natural products available to grow healthier and antibiotic free meat animals. In the context of exploring natural and sustainable resource of alternative to antibiotics, the biochemical milieu of eggshell membranes (ESM) were analyzed by using mass spectrometry techniques including matrix assisted laser desorption ionization …

Vaccines And Airline Travel: A Federal Role To Protect The Public Health, Christopher Robertson

Faculty Scholarship

This Article explores two ways in which airline travel is an important vector for the spread of infectious disease, and argues that airlines have market-based and liability-based reasons to require that passengers be vaccinated. Going further, the Article explores whether the federal government has the legal and constitutional authority — especially under the Commerce Clause — to encourage or mandate that airlines implement such a vaccine screen. By disrupting the spread of disease at key network nodes where individuals interact and then connect with other geographic regions, and by creating another incentive for adult vaccination, an airline vaccine screen could …

Phenotypic And Molecular Analysis Of The Effect Of 20-Hydroxyecdysone On The Human Filarial Parasite Brugia Malayi, Amruta S. Mhashilkar, Swamy R. Adapa, Rays H.Y. Jiang, Steven A. Williams, Weam I. Zaky, Barton E. Slatko, Ashley N. Luck, Andrew Moorhead, Thomas R. Unnasch

Biological Sciences: Faculty Publications

A homologue of the ecdysone receptor has been identified and shown to be responsive to 20- hydroxyecdysone in Brugia malayi. However, the role of this master regulator of insect development has not been delineated in filarial nematodes. Gravid adult female B. malayi cultured in the presence of 20-hydroxyecdysone produced significantly more microfilariae and abortive immature progeny than control worms, implicating the ecdysone receptor in regulation of embryogenesis and microfilarial development. Transcriptome analyses identified 30 genes whose expression was significantly up-regulated in 20-hydroxyecdysone-treated parasites compared with untreated controls. Of these, 18% were identified to be regulating transcription. A comparative proteomic analysis …

Association Of Ecological Factors With Rift Valley Fever Occurrence And Mapping Of Risk Zones In Kenya, Gladys Mosomtai, Magnus Evander, Per Sandström, Clas Ahlm, Rosemary Sang, Osama Ahmed Hassan, Hippolyte Affognon, Tobias Landmann

All Peer-Reviewed Publications

Objective: Rift Valley fever (RVF) is a mosquito-borne infection with great impact on animal and human health. The objectives of this study were to identify ecological factors that explain the risk of RVF outbreaks in eastern and central Kenya and to produce a spatially explicit risk map. Methods: The sensitivity of seven selected ecological variables to RVF occurrence was assessed by generalized linear modelling (GLM). Vegetation seasonality variables (from normalized difference vegetation index (NDVI) data) and 'evapotranspiration' (ET) (metrics) were obtained from 0.25-1 km MODIS satellite data observations; 'livestock density' (N/km2), 'elevation' (m), and 'soil ratio' (fraction of all significant …

Friendly Fire: Biological Functions And Consequences Of Chromosomal Targeting By Crispr-Cas Systems, Gary E. Heussler, George A. O'Toole

Dartmouth Scholarship

Clustered regularly interspaced short palindromic repeat (CRISPR)-associated (Cas) systems in bacteria and archaea target foreign elements, such as bacteriophages and conjugative plasmids, through the incorporation of short sequences (termed spacers) from the foreign element into the CRISPR array, thereby allowing sequence-specific targeting of the invader. Thus, CRISPR-Cas systems are typically considered a microbial adaptive immune system. While many of these incorporated spacers match targets on bacteriophages and plasmids, a noticeable number are derived from chromosomal DNA. While usually lethal to the self-targeting bacteria, in certain circumstances, these self-targeting spacers can have profound effects in regard to microbial biology, including functions …

Probiotics For Treatment And Prevention Of Urogenital Infections In Women: A Systematic Review, Lisa Hanson, Leona Vandevusse, Martha G. Jermé, Cybéle L. Abad, Nasia Safdar

College of Nursing Faculty Research and Publications

Introduction

Probiotics are a complementary and integrative therapy useful in the treatment and prevention of urogenital infections in women. This study extends the work of researchers who systematically investigated the scientific literature on probiotics to prevent or treat urogenital infections.

Methods

A systematic review was conducted to determine the efficacy of probiotics for prevention and/or treatment of urogenital infections in adult women from January 1, 2008, through June 30, 2015. We searched in CINAHL, MEDLINE, Cochrane Central Register of Controlled Trials, Web of Science, Dissertations and Theses, and Alt-HealthWatch. After removing duplicates and studies that did not meet inclusion criteria, …