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Rapid, Low-Cost Biosensor For Water-Borne Pathogens In Low-Resource Environments, Kimberly Cribbs

Chemical Engineering Undergraduate Honors Theses

When testing water quality, “microbiological results typically require a minimum of 24 hours to complete” due to reliance on the traditional and inexpensive cultivation method. In 2000, an isothermal DNA amplification method termed loop-mediated isothermal amplification (LAMP) was invented allowing for microbiological assessments to be performed in less than one hour without the need for an expensive thermocycler. In 2013, the World Health Organization reported LAMP as an “attractive diagnostic platform for resource-poor settings: it is fast [15-40 min], isothermal [requiring only a heat block], robust to inhibitors and reaction conditions that usually adversely affect PCR methods, and it generates …

Examination Of Klebsiella Pneumoniae 5'-Methylthioadenosine/S-Adenosylhomocysteine Nucleosidase And 5-Methylthioribose Kinase, Jason Alan Stonick

Boise State University Theses and Dissertations

Klebsiella pneumoniae is an opportunistic bacterial pathogen that is emerging as a major global threat as an infectious agent. This organism, along with many other pathogens, possesses a broad suite of antibiotic resistances that can make treatment exceedingly difficult. As such, the impetus for creating novel antibiotics is at an all-time high despite the fact that pharmaceutical investment into drug development is at an all-time low. S-adenosylmethionine (SAM) is a universally utilized metabolite involved in a wide array of biosynthetic processes, and whose products are catabolized by a different set of enzymes in mammals than used by many bacteria, protozoa, …

Computational And Experimental Investigations Towards Improving Organic And Dye-Sensitized Semiconductor Solar Cells, Nathan D. Colley

Honors Theses

The global energy consumption is expected to double by 2050. Therefore, alternative energy sources such as solar power will have to be utilized. This work seeks to improve next generation solar cells, namely organic solar cells (OSCs) and dye-sensitized semiconductor solar cells (DSSCs), using theoretical and experimental approaches. Computational studies were done on electron acceptor and electron donor molecules that have potential use in OSCs and DSSCs. An improved Density Functional Theory (DFT) method that better represents experimental cyclic voltammetry was developed to predict the electronic properties of these molecules. The electronic data of the electron acceptor molecules were used …

Cancer Among Asian American Population In The United States: Incidence And Survival Disparities, Hongbin Jin

UNLV Theses, Dissertations, Professional Papers, and Capstones

Part I

Cancer incidence disparities exist among specific Asian American populations. However, the existing reports exclude data from large metropoles like Chicago, Houston, and New York. Moreover, incidence rates by subgroup have been underestimated due to the exclusion of Asians with unknown subgroup. Cancer incidence data for 2009 to 2011 for eight states accounting for 68% of the Asian American population were analyzed. Race for cases with unknown subgroup was imputed using stratified proportion models by sex, age, cancer site, and geographic regions. Age-standardized incidence rates were calculated for 17 cancer sites for the six largest Asian subgroups. Our analysis …

Microbial Contamination Of Ice At Food Establishments In Las Vegas, Nevada, Jerry Wills

UNLV Theses, Dissertations, Professional Papers, and Capstones

When food establishments make ice for consumption, it is important to use water free of pathogens and to employ hygienic practices. Freezing can reduce the number of culturable microorganisms present in water but does not eliminate them completely. Coliform bacteria are used as an indicator of water quality and are used to test for fecal contamination. The U.S. Environmental Protection Agency (EPA) establishes standards for drinking water, and has set a goal for zero total coliforms. Another commonly used tool to measure water quality is the heterotrophic plate count (HPC). The EPA limit for HPC is 500 colony forming units …

The Rise And Fall Of Efavirenz, David Cluck, Paul Lewis, Spencer H. Durham, E. Kelly Hester

ETSU Faculty Works

No abstract provided.

Design For Mosquito Abundance, Diversity, And Phenology Sampling Within The National Ecological Observatory Network, D. Hoekman, Y. P. Springer, C. M. Barker, R. Barrera, M. S. Blackmore, W. E. Bradshaw, D. H. Foley, H. S. Ginsberg, M. H. Hayden, C. M. Holzapfel, S. A. Juliano, L. D. Kramer, S. L. Ladeau, Todd P. Livdahl, C. G. Moore, R. S. Nasci, W. K. Reisen, H. M. Savage

Biology

The National Ecological Observatory Network (NEON) intends to monitor mosquito populations across its broad geographical range of sites because of their prevalence in food webs, sensitivity to abiotic factors, and relevance for human health. We describe the design of mosquito population sampling in the context of NEON's long-term continental scale monitoring program, emphasizing the sampling design schedule, priorities, and collection methods. Freely available NEON data and associated field and laboratory samples, will increase our understanding of how mosquito abundance, demography, diversity, and phenology are responding to land use and climate change.

Erratum: In the paper by Hoekman et al. (“Design …

Ecological Niche Modeling Of The North American Giant Salamander: Predicting Current And Future Potential Distributions And Examining Environmental Influences, Selena S. Roark

Electronic Theses and Dissertations

North American Giant Salamanders (Cryptobranchus alleganiensis), commonly known as hellbenders, have been experiencing a population decline for decades due to human influences, such as pollution and habitat destruction. Many efforts are underway to save the hellbender but their entire potential geographical range has not been well-studied. Currently, hellbender populations are delineated by county boundaries and are on the International Union for Conservation of Nature Red List. The Genetic Algorithm for Rule-set Production, an Ecological Niche Model, was used to model the current hellbender potential distribution at a macro-scale under two different environmental scenarios. Additionally, future potential distributions were …

Modeling And Prediction Of Oyster Norovirus Outbreaks Along Gulf Of Mexico Coast, Jiao Wang, Zhiqiang Deng

Faculty Publications

Background: Oyster norovirus outbreaks often pose high risks to human health. However, little is known about environmental factors controlling the outbreaks, and little can be done to prevent the outbreaks because they are generally considered to be unpredictable. oBjective: We sought to develop a mathematical model for predicting risks of oyster norovirus outbreaks using environmental predictors. Methods: We developed a novel probability-based Artificial Neural Network model, called NORF model, using 21 years of environmental and norovirus outbreak data collected from Louisiana oyster harvesting areas along the Gulf of Mexico coast, USA. The NORF model involves six input variables that were …

Where Healthcare And Policing Converge: How Georgia Law Promotes Evasion Of Financial Responsibility For Indigent Arrestees' And Municipal Inmates' Medical Care, L. Taylor Hamrick

Mercer Law Review

When a law enforcement officer arrests an injured or visibly sick person, the officer typically transports the arrestee directly to a hospital for treatment prior to formal booking in a jail or detention facility. Indeed, convicted inmates, pretrial detainees, and arrestees have a constitutional right to receive necessary medical care while in police custody. However, the United States Supreme Court has distinguished a government's constitutional obligation to provide necessary medical care from a duty to pay for such care.' Instead, the Supreme Court has held that a governmental entity must pay for medical treatment of a person in its custody …

Impacts Of Laurel Wilt Disease On Native Persea Of The Southeastern United States, Timothy M. Shearman

All Dissertations

Laurel Wilt Disease (LWD) has caused severe mortality in native Persea species of the southeastern United States since it was first detected in 2003. This study was designed to document the range-wide population impacts to LWD, as well as the patterns of mortality and regeneration in Persea ecosystems. I used Forest Inventory and Analysis (FIA) data from the U.S. Forest Service to estimate Persea borbonia (red bay) populations from 2003 to 2011 to see if any decline could be observed since the introduction of LWD causal agents. Population estimates from 2003 to 2011 suggest that the population is declining. The …

Genotoxicity Of Graphene In Escherichia Coli, Ananya Sharma

Graduate Theses and Dissertations

Rapid advances in nanotechnology necessitate assessment of the safety of nanomaterials in the resulting products and applications. One key nanomaterial attracting much interest in many areas of science and technology is graphene. Graphene is a one atom thick carbon allotrope arranged in a two-dimensional honeycomb lattice. In addition to being extremely thin, graphene has several extraordinary physical properties such as its exceptional mechanical strength, thermal stability, and high electrical conductivity. Graphene itself is relatively chemically inert and therefore pristine graphene must undergo a process called functionalization, which is combination of chemical and physical treatments that change the properties of graphene, …

The Transfer Of Commensal And Transient Bacteria Into Deep Tissue Using Dry Needling Needles Through Agar Media Designed To Mimic The Elasticity Of Human Skin, Denisse N. Breton Guerrero

Honors Theses

Needle insertion into skin for the purpose of alleviating muscle pain began with the emergence of traditional Chinese medicinal (TCM) acupuncture. “Dry needling”, a recent alternative to acupuncture, involves inserting a monofilament needle down into the deep tissue of a patient’s skin in order to reach the affected muscle. Despite its effectiveness and low risk of infection, dry needling remains questionable with respect to its safety and the lack of research to confirm the low risk of bacterial transmission via the dry needling needles. In this microbiological study, highly concentrated Tryptic Soy Agar was utilized to simulate the elasticity of …

Effect Of Body Mass Index On Outcome Of Labour Induction, Farheen Yousuf, Tahira Naru, Sana Sheikh

Department of Obstetrics & Gynaecology

The retrospective study to explore the adverse effect of obesity on pregnancy and labour was conducted at Aga Khan University Hospital, Karachi, Pakistan, and comprised data of all patients booked between 12-14 weeks and required induction of labour from January 1 to December 31, 2012. Women were grouped into two body mass index categories: normal weight (23 kg/m2). Obesity increased the risk of development of gestational hypertension and diabetes. Therefore obese women were more likely to be induced due to medical indication whether primiparous or multiparous adjusted odds ratio =2.89(95% confidence interval 1.29-6.48) and 2.77 (95% confidence interval 1.07-7.19) respectively. …

Biomolecular Signatures Of Severe Malarial Anemia, Zachary Shamin Karim

Biomedical Sciences ETDs

Plasmodium falciparum-associated severe malarial anemia [SMA, hemoglobin (Hb)< 5.0g/dL] is a leading cause of morbidity and mortality in African children. Examination of the host immune response and underlying genotypic traits that condition SMA can offer an improved understanding of malaria pathogenesis. Previous investigations have suggested that SMA in high transmission regions is due to impaired erythropoiesis hemolysis and erythrocyte destruction. In the holoendemic transmission region of western Kenya our studies have shown that dyserythropoiesis drives and conditions SMA through poorly understood molecular pathways. Pediatric studies have shown that early erythroid cell populations as well as reticulocyte production is greatly reduced in children with SMA. The driving force of malaria pathogenesis is multifaceted and the mechanisms that promote erythropoietic dysfunction are still being understood. We hypothesize that SMA is conditioned by the immune response through underlying genetic factors that influence erythropoiesis resulting in a exacerbated disease state. To that end the studies described in this manuscript focus on the inflammatory chemokines, monokine induced by gamma (MIG) and interferon gamma-induced protein (IP10) as well as the hemoglobin-coding gene, hemoglobin alpha 2 (HBA2) in the context of SMA and dyserythropoiesis. The first study described herein, identified MIG and IP10 as important mediators associated with SMA. In this study we examined circulating MIG and I10 profiles in non-SMA and SMA children and then genotyped and constructed haplotypes between 5 individual polymorphic variants within the promoter regions for the MIG and IP10 for the entire study cohort (n=1,600). The second study described in this manuscript, utilized a global approach where genome wide association studies as well as whole-genome transcriptomics identified novel molecular pathways between non-SMA and SMA children. These analyses revealed that the expression of the hemoglobin-coding gene, HBA2 is significantly modulated between populations. We then examined these findings closer and genotyped a larger population for 2 promoter variants with the same approach described in the first study. Regression analysis of genotype and haplotype data for both studies revealed significant findings where several variant haplotypes correlated with SMA outcomes and erythropoietic response. Lastly, we used an in vitro model of erythropoiesis to determine if recombinant human MIG and IP10 as well as pediatric study serum (non-SMA and SMA) influence erythroid function. Our results from these studies revealed that pediatric serum from children with SMA influenced cell viability as well as erythroid lineage when we examined cell surface erythroid markers. A similar result was also seen in cell populations that were treated with the inflammatory mediators, MIG, and IP10 which reflected our proposal that serum from children with SMA and inflammatory mediators (MIG and IP10) infringe upon erythroid cell maturation and viability in vivo. Together the results from these studies independently verify the influence of biomolecular markers and mediators on SMA outcomes.

Pulmonary Bcg Vaccination For Uniform Protection Against Tuberculosis In Environmental Mycobacteria Endemic Regions, Dominique Nicole Price

Biomedical Sciences ETDs

Currently, over one-third of the global population is infected with tuberculosis (TB), one of the world's most deadly infectious diseases. The current vaccine against TB, Bacille Calmette-Guérin (BCG), is a live attenuated vaccine that is intradermally administered in over 150 countries around the world. Despite its widespread use, the vaccine has a variable protective efficacy of 0-80%, with the lowest efficacy rates in regions of the world where TB is most prevalent. This variability is partially due to environmental mycobacteria (EM) found in soil and water sources in areas of the world where BCG efficacy is poor. The research hypothesis …

Protein Phosphatase 1 Abrogates Irf7-Mediated Type I Ifn Response In Antiviral Immunity, Ling Wang, Juan Zhao, Junping Ren, Kenton H. Hall, Jonathan P. Moorman, Zhi Q. Yao, Shunbin Ning

ETSU Faculty Works

Interferon (IFN) regulatory factor 7 (IRF7) plays a key role in the production of IFN‐α in response to viral infection, and phosphorylation at IRF7 C‐terminal serine sites is prelude to its function. However, phosphatases that negatively regulate IRF7 phosphorylation and activity have not been reported. In this study, we have identified a conserved protein phosphatase 1 (PP1)‐binding motif in human and mouse IRF7 proteins, and shown that PP1 physically interacts with IRF7. Exogenous expression of PP1 subunits (PP1α, β, or γ) ablates IKKε‐stimulated IRF7 phosphorylation and dramatically attenuates IRF7 transcriptional activity. Inhibition of PP1 activity significantly increases IRF7 phosphorylation and …

Development Of Arginyl-Trna Synthetase From Pseudomonas Aeruginosa As A Platform To Screen For Inhibitors Of Protein Synthesis, Daniel Cantu

Theses and Dissertations

Pseudomonas aeruginosa is a Gram- negative bacteria that is the leading cause of mortality in immunocompromised patients. Arginyl-tRNA synthetase (ArgRS) is an enzyme that catalyzes the covalent attachment of arginine to the cognate tRNAArg during protein synthesis. Scintillation proximity assays (SPA) were adapted to the aminoacylation assay and used to screen, one natural compound library (800) and one synthetic compound library (890) to identify inhibitors of the activity of ArgRS. Five compounds were identified which inhibited greater than 50% of enzymatic activity, two of these compounds (BT04F10 and BT11F03) exhibited promising MICs against Gram+ bacteria and moderate activity against Gram- …

Tick-, Mosquito-, And Rodent-Borne Parasite Sampling Designs For The National Ecological Observatory Network, Yuri P. Springer, David Hoekman, Pieter T.J. Johnson, Paul A. Duffy, Rebecca A. Hufft, David T. Barnett, Brian F. Allan, Brian R. Amman, Christopher M. Barker, Roberto Barrera, Charles B. Beard, Lorenza Beati, Mike Begon, Mark S. Blackmore, William E. Bradshaw, Dustin Brisson, Charles H. Calisher, James E. Childs, Maria A. Diuk-Wasser, Richard J. Douglass, Rebecca J. Eisen, Desmond H. Foley, Janet E. Foley, Holly D. Gaff, Scott L. Gardner, Howard S. Ginsberg, Gregory E. Glass, Sarah A. Hamer, Mary H. Hayden, Brian Hjelle, Christina M. Holzapfel, Steven A. Juliano, Todd P. Livdahl

Biology

Parasites and pathogens are increasingly recognized as significant drivers of ecological and evolutionary change in natural ecosystems. Concurrently, transmission of infectious agents among human, livestock, and wildlife populations represents a growing threat to veterinary and human health. In light of these trends and the scarcity of long-term time series data on infection rates among vectors and reservoirs, the National Ecological Observatory Network (NEON) will collect measurements and samples of a suite of tick-, mosquito-, and rodent-borne parasites through a continental-scale surveillance program. Here, we describe the sampling designs for these efforts, highlighting sampling priorities, field and analytical methods, and the …

The Efficacy Of Zinc And Manganese In Controlling Methicillin-Resistant Staphylococcus Aureus Wound Infections In Vitro, Patrick B. Lau

Honors Scholar Theses

The emergence of Methicillin-Resistant Stapylococcus aureus has triggered an increased interest in finding alternative natural antimicrobials to control the pathogen and combat growing antibiotic resistance. This study investigated the antimicrobial effect of two naturally occurring essential minerals, zinc (Zn) and manganese (Mn) on MRSA for potential application in wound infections. The sub inhibitory concentration (SIC) and minimum inhibitory concentration (MIC) of Zn and Mn against MRSA were determined. The effect of MIC and 2x MIC of Zn and Mn in increasing MRSA susceptibility to oxacillin, and the effect of SIC and MIC of these minerals on MRSA cell adherence and …

Bacteriophage Virus-Like Particles As Vaccine Platforms: From Heart Disease To Malaria, Erin Crossey

Biomedical Sciences ETDs

Virus-like particles (VLPs) make excellent vaccines. They are non-infectious, often easy to produce in bacterial expression systems, and highly immunogenic. The latter feature is granted by the regularity of the capsid structure, which presents viral (or other) epitopes as dense, highly repetitive arrays that strongly stimulate B cells. VLPs can be used as the basis for vaccines targeting the virus from which they were derived, or they can be used as platforms to display practically any short peptide epitope in a multivalent format. Foreign peptides displayed on VLPs exhibit the same high immunogenicity as unmodified VLPs. Even self-antigens, normally subject …

Stabilization Of Gp120 Outer Domain For Hiv-1-1 Vaccine Immunogen Design, Duoyi Hu

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

The sole envelope glycoprotein spike on the surface of Human Immunodeficiency Virus (HIV-1) is composed of two subunits: gp120 and gp41. The gp120 consists of three domains: the inner domain, outer domain and bridging sheet, to which the viral primary receptor CD4 binds and induces a conformational change in gp120 to expose the co-receptor binding site. Previous studies have found that the outer domain of gp120 is relatively more stable than the inner domain and bridging sheet. When superimposed, the co-crystal structures of CD4-gp120 complex and VRC01-gp120 complex revealed that the CD4-binding site (CD4-BS) antibody VRC01 actually mainly bound to …

Jazz Epidemics And Deep Set Diseases: The De-Pathologization Of The Black Body In The Work Of Three Harlem Renaissance Writers, Shane C. Hunter

Department of English: Dissertations, Theses, and Student Research

This dissertation argues that the Harlem Renaissance was, in part, a response to Victorian-era medical and scientific racism, and that the three writers on which it centers, Langston Hughes (1902-1967), Wallace Thurman (1902-1934), and Richard Bruce Nugent (1906-1987), participated in subverting these racist discourses. I focus on elements of their creative work that de-pathologize the black body. Specifically, I consider how these writers undermine Victorian-era medical racism that had, by the 1920s, come to inform American racial politics. Hughes’s, Thurman’s, and Nugent’s work from the mid-1920s to the early 1930s is at least partly concerned with undermining medically racist ideology …

Performance Benchmarks For Scholarly Metrics Associated With Fisheries And Wildlife Faculty, Robert K. Swihart, Mekala Sundaram, Tomas O. Höök, James Andrew Dewoody, Kenneth F. Kellner

Department of Forestry & Natural Resources Faculty Publications

Research productivity and impact are often considered in professional evaluations of academics, and performance metrics based on publications and citations increasingly are used in such evaluations. To promote evidence-based and informed use of these metrics, we collected publication and citation data for 437 tenure-track faculty members at 33 research-extensive universities in the United States belonging to the National Association of University Fisheries and Wildlife Programs. For each faculty member, we computed 8 commonly used performance metrics based on numbers of publications and citations, and recorded covariates including academic age (time since Ph.D.), sex, percentage of appointment devoted to research, and …

A Novel And Rapid Staphylococcus Aureus Bacterial Identification Method Utilizing Immunomagnetic Beads And Single Cell Laser-Light Scattering, Kaylagh Hollen

All NMU Master's Theses

Staphylococcus aureus is the most commonly isolated human associated bacterial pathogen. It plays an important role in skin and soft-tissue infections, pneumonia, endocarditis, osteomyelitis, foreign-body infections, and sepsis. S. aureus diagnosis and treatment requires a minimum of 24-48. With this in mind, previous studies suggest that faster pathogen identification has been linked to improved patient outcomes. Improved patient outcomes including a reduction in hospitalization time, decreased risk of nosocomial infections, and decreased in medical costs. The impact of faster identification on patient outcome has led us to develop an alternative method of S. aureus identification via ImmunoMagnetic Separation (IMS) and …

The Impact Of Mycobacterium Leprae: A Comprehensive Meta-Analysis Of The Paleopathological Literature, Mallory Alexis Schreier

Boise State University Theses and Dissertations

Leprosy, caused by the bacteria Mycobacterium leprae and Mycobacterium lepromatosis, is a chronic, infectious disease that eventually causes disfiguring skin lesions, nerve damage, and muscle weakness. Even though leprosy has been nearly eliminated in many parts of the world today, it remains endemic in India, Myanmar, Nepal, Brazil, and a few African countries. Unfortunately, this infectious disease is not limited to just modern populations. In the past, leprosy spread globally and was a pervasive, degenerating disease. The literature traces leprosy back to 1550 BCE although there is possible skeletal evidence of leprosy in Rajasthan, India from 2000 BCE, suggesting …

Reading Between The Lines: Indicators Of Developmental Stress In Prehistoric Ohio Valley Children From Linear Enamel Hypoplasias, Emily Moes

Boise State University Theses and Dissertations

A large body of research in bioarchaeology focuses on the changes in the human skeleton associated with the introduction of agriculture. It is assumed that the intensification of agriculture results in an increase in physiological stress and poor health. However, previous studies have shown that stress experiences cannot be generalized by subsistence strategy and prevalence comparisons alone. Rather, age-at and duration of stress events are necessary to construct patterns of health.

Linear enamel hypoplasias (LEH) of subadult permanent dentition serve as a proxy for understanding health and stress in archaeological populations. LEH are defects in enamel, characterized by an increased …

Investigation Of The Tailoring Steps In Pradimicin Biosynthesis, Kandy L. Napan

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This research focused on the investigation of the late steps in the biosynthetic pathway of the novel antifungal and antiviral pradimicins A-C. Pradimicins were first isolated from the soil bacterium Actinomadura hibisca. These bioactive molecules are assembled by a type II polyketide biosynthetic pathway. Although the biosynthetic gene cluster of pradimicin has been identified, the functions of the biosynthetic genes and how they work collaboratively to form the final structures of pradimicins remain unknown. This research aims to functionally characterize the enzymes involved in the late steps of the biosynthetic route.

The early biosynthetic steps of pradimicins have been …

Immunomodulation Of Breast Cancer Cells For Whole Tumor Vaccination, Kristina G. Maxwell

Biomedical Engineering Undergraduate Honors Theses

Hematogenous metastasis causes 90% of breast cancer-related deaths.Current therapies include chemotherapy and irradiation following surgery. These therapies are very harmful to the human body and do not elicit an anti-tumor immune response. To create a novel therapeutic, an autologous vaccine increasing the immunogenicity of non immunogenic breast cancer cell lines has been proposed.

To create this vaccine, 4T1 mouse mammary breast cancer cells have been selected as the desired cell line to treat. They are non immunogenic and highly invasive. In order to increase their immunogenicity, first projected, was the addition of cytokines to 4T1 cells to increase the expression …

Contribution Of Probiotics Streptococcus Salivarius Strains K12 And M18 To Oral Health In Humans: A Review, Turner A. Stowik

Honors Scholar Theses

The overgrowth and disequilibrium of pathogenic microorganism species both native and non-native to the oral cavity can manifest into a variety of different oral diseases, pathologies, and afflictions in humans, including dental caries, gingivitis, pharyngitis, halitosis, and oral candidiasis. Two bacterial strains with clinically-significant probiotic applications in curtailing the pathogenic bacterial growth involved in these conditions are Streptococcus salivarius strain K12 and Streptococcus salivarius strain M18. To summarize the most up-to-date in vitro, in vivo, and clinical research findings, administration of these S. salivarius strains typically in the form of probiotic lozenges results in colonization, reduction in inflammatory measures, …