Digital Commons Network^™

Liposomes With Diverse Compositions Are Protected During Desiccation By Lea Proteins From Artemia Franciscana And Trehalose, Daniel S. Moore, Richard Hansen, Steven C. Hand

Faculty Publications

© 2015 Published by Elsevier B.V. Intracellular accumulation of Late Embryogenesis Abundant (LEA) proteins and the disaccharide trehalose is associated with cellular desiccation tolerance in a number of animal species. Two LEA proteins from anhydrobiotic embryos of the brine shrimp Artemia franciscana were tested for the ability to protect liposomes of various compositions against desiccation-induced damage in the presence and absence of trehalose. Damage was assessed by carboxyfluorescein leakage after drying and rehydration. Further, using a cytoplasmic-localized (AfrLEA2) and a mitochondrial-targeted (AfrLEA3m) LEA protein allowed us to evaluate whether each may preferentially stabilize membranes of a particular lipid composition based …

Crawling With Virus: Translational Insights From A Neonatal Mouse Model On The Pathogenesis Of Respiratory Syncytial Virus In Infants, Dahui You, Jordy Saravia, David Siefker, Bishwas Shrestha, Stephania A. Cormier

Faculty Publications

© 2015, American Society for Microbiology. The infant immune response to respiratory syncytial virus (RSV) remains incompletely understood. Here we review the use of a neonatal mouse model of RSV infection to mimic severe infection in human infants. We describe numerous age-specific responses, organized by cell type, observed in RSV-infected neonatal mice and draw comparisons (when possible) to human infants.

Autophagy Is Essential For Ultrafine Particle-Induced Inflammation And Mucus Hyperproduction In Airway Epithelium, Zhi Hua Chen, Yin Fang Wu, Ping Li Wang, Yan Ping Wu, Zhou Yang Li, Yun Zhao, Jie Sen Zhou, Chen Zhu, Chao Cao, Yuan Yuan Mao, Feng Xu, Bei Bei Wang, Stephania A. Cormier, Song Min Ying, Wen Li, Hua Hao Shen

Faculty Publications

© 2016 Taylor & Francis Group, LLC. Environmental ultrafine particulate matter (PM) is capable of inducing airway injury, while the detailed molecular mechanisms remain largely unclear. Here, we demonstrate pivotal roles of autophagy in regulation of inflammation and mucus hyperproduction induced by PM containing environmentally persistent free radicals in human bronchial epithelial (HBE) cells and in mouse airways. PM was endocytosed by HBE cells and simultaneously triggered autophagosomes, which then engulfed the invading particles to form amphisomes and subsequent autolysosomes. Genetic blockage of autophagy markedly reduced PM-induced expression of inflammatory cytokines, e.g. IL8 and IL6, and MUC5AC in HBE cells. …

Plastic And Evolved Responses To Global Change: What Can We Learn From Comparative Transcriptomics?, Melissa B. Debiasse, Morgan W. Kelly

Faculty Publications

© The American Genetic Association. 2015. All rights reserved. Physiological plasticity and adaptive evolution may facilitate persistence in a changing environment. As a result, there is an interest in understanding species' capacities for plastic and evolved responses, and the mechanisms by which these responses occur. Transcriptome sequencing has become a powerful tool for addressing these questions, providing insight into otherwise unobserved effects of changing conditions on organismal physiology and variation in these effects among individuals and populations. Here, we review recent studies using comparative transcriptomics to understand plastic and evolutionary responses to changing environments. We focus on 2 areas where …

Biological Control Activities Of Rice-Associated Bacillus Sp. Strains Against Sheath Blight And Bacterial Panicle Blight Of Rice, Bishnu K. Shrestha, Hari Sharan Karki, Donald E. Groth, Nootjarin Jungkhun, Jong Hyun Ham

Faculty Publications

Potential biological control agents for two major rice diseases, sheath blight and bacterial panicle blight, were isolated from rice plants in this study. Rice-associated bacteria (RABs) isolated from rice plants grown in the field were tested for their antagonistic activities against the rice pathogens, Rhizoctonia solani and Burkholderia glumae, which cause sheath blight and bacterial panicle blight, respectively. Twenty-nine RABs were initially screened based on their antagonistic activities against both R. solani and B. glumae. In follow-up retests, 26 RABs of the 29 RABs were confirmed to have antimicrobial activities, but the rest three RABs did not reproduce any observable …

Pteridine Levels And Head Weights Are Correlated With Age And Colony Task In The Honey Bee, Apis Mellifera, Frank D. Rinkevich, Joseph W. Margotta, Jean M. Pittman, James A. Ottea, Kristen B. Healy

Faculty Publications

Background. The age of an insect strongly influences many aspects of behavior and reproduction. The interaction of age and behavior is epitomized in the temporal polyethism of honey bees in which young adult bees perform nurse and maintenance duties within the colony, while older bees forage for nectar and pollen. Task transition is dynamic and driven by colony needs. However, an abundance of precocious foragers or overage nurses may have detrimental effects on the colony. Additionally, honey bee age affects insecticide sensitivity. Therefore, determining the age of a set of individual honey bees would be an important measurement of colony …

Lsu General Catalog 2016-2017, Louisiana State University And Agricultural And Mechanical College

LSU General Catalog

The LSU General Catalog describes all undergraduate and graduate departments and programs with degree requirements and courses offered for each one. The LSU General Catalog includes information on registration and financial aid as well as academic services offered to all students.

Front Matter

1650-1850: Ideas, Aesthetics, and Inquiries in the Early Modern Era

No abstract provided.

Picturesque Movement In Ann Radcliffe's Novels, Julien Morel

1650-1850: Ideas, Aesthetics, and Inquiries in the Early Modern Era

No abstract provided.

Swift's Concept Of Physical Exercise: The Gravel Path To Human Perfection, Esther F. Sommer

1650-1850: Ideas, Aesthetics, and Inquiries in the Early Modern Era

No abstract provided.

Stability Turned Into Motion Erasmus Darwin's Recourse To Ovid's Metamorphoses, Sonja Fielitz

1650-1850: Ideas, Aesthetics, and Inquiries in the Early Modern Era

No abstract provided.

Turning Round Or Standing Still Movement And Stasis In Defoe, Pope, And Sterne, Allan Ingram, Hélène Dachez

1650-1850: Ideas, Aesthetics, and Inquiries in the Early Modern Era

No abstract provided.

The Creative Cost Of Jane Austen's Move To Bath, Gerald J Butler

1650-1850: Ideas, Aesthetics, and Inquiries in the Early Modern Era

No abstract provided.

Yorick And Lovelace Physical Manifestations And Internal E-Motions, Maryam Ghabris

1650-1850: Ideas, Aesthetics, and Inquiries in the Early Modern Era

No abstract provided.

Cook's Ark Animals On The Move In The Service Of Empires, Bärbel Czennia

1650-1850: Ideas, Aesthetics, and Inquiries in the Early Modern Era

No abstract provided.

Picturing The Museum: How America's Leading Art Museums Use Instagram As A Public Relations Tool, Hannah S. Mclain

Honors Capstones

No abstract provided.

Cognitive Phenotypes And The Evolution Of Animal Decisions, Tamra C. Mendelson, Courtney L. Fitzpatrick, Mark E. Hauber, Charles H. Pence, Rafael L. Rodríguez, Rebecca J. Safran, Caitlin A. Stern, Jeffrey R. Stevens

Faculty Publications

Despite the clear fitness consequences of animal decisions, the science of animal decision making in evolutionary biology is underdeveloped compared with decision science in human psychology. Specifically, the field lacks a conceptual framework that defines and describes the relevant components of a decision, leading to imprecise language and concepts. The ‘judgment and decision-making’ (JDM) framework in human psychology is a powerful tool for framing and understanding human decisions, and we apply it here to components of animal decisions, which we refer to as ‘cognitive phenotypes’. We distinguish multiple cognitive phenotypes in the context of a JDM framework and highlight empirical …

Eating In The Side Room: Food, Archaeology, And African American Identity, Rachel A. Snell

Civil War Book Review

Diet, Cuisine, and the Creation of African-American Identity

Through the lens of food, Mark S. Warner’s Eating in the Side Room: Food, Archaeology, and African American Identity demonstrates the influence of mass consumer culture on African-American diets and the role played by food....

Acetylation Of Androgen Receptor By Ard1 Promotes Dissociation From Hsp90 Complex And Prostate Tumorigenesis, John S. Depaolo, Zehua Wang, Jianhui Guo, Guanyi Zhang, Chiping Qian, Haitao Zhang, Jovanny Zabaleta, Wanguo Liu

Faculty Publications

Prostate cancer is an androgen receptor (AR)-driven disease and post-translational modification of AR is critical for AR activation. We previously reported that Arrestdefective protein 1 (ARD1) is an oncoprotein in prostate cancer. It acetylates and activates AR to promote prostate tumorigenesis. However, the ARD1-targeted residue within AR and the mechanisms of the acetylation event in prostate tumorigenesis remained unknown. In this study, we show that ARD1 acetylates AR at lysine 618 (K618) in vitro and in vivo. An AR construct with the charged lysine substitution by arginine (AR-618R) reduces RNA Pol II binding, AR transcriptional activity, prostate cancer cell growth, …

Rearrangement And Evolution Of Mitochondrial Genomes In Parrots, Jessica R. Eberhard, Timothy F. Wright

Faculty Publications

© 2015 Elsevier Inc. Mitochondrial genome rearrangements that result in control region duplication have been described for a variety of birds, but the mechanisms leading to their appearance and maintenance remain unclear, and their effect on sequence evolution has not been explored. A recent survey of mitochondrial genomes in the Psittaciformes (parrots) found that control region duplications have arisen independently at least six times across the order. We analyzed complete mitochondrial genome sequences from 20 parrot species, including representatives of each lineage with control region duplications, to document the gene order changes and to examine effects of genome rearrangements on …

Cryopreservation In Fish: Current Status And Pathways To Quality Assurance And Quality Control In Repository Development, Leticia Torres, E. Hu, Terrence R. Tiersch

Faculty Publications

Cryopreservation in aquatic species in general has been constrained to research activities for more than 60 years. Although the need for application and commercialisation pathways has become clear, the lack of comprehensive quality assurance and quality control programs has impeded the progress of the field, delaying the establishment of germplasm repositories and commercial-scale applications. In this review we focus on the opportunities for standardisation in the practices involved in the four main stages of the cryopreservation process: (1) source, housing and conditioning of fish; (2) sample collection and preparation; (3) freezing and cryogenic storage of samples; and (4) egg collection …

Understanding The Effect Of Extrusion Processing Parameters On Physical, Nutritional And Rheological Properties Of Soy White Flakes Based Aquafeed In A Single Screw Extruder, Sushil Kumar Singh

Electronic Theses and Dissertations

The rapid expansion of aquaculture has increased the demand for aquafeed. As fishmeal is expensive, alternative plant based protein sources such as soybean has shown to be a good alternative for aquafeed production. The present study was done to investigate the effect of soy white flakes (SWF) as an alternative source of protein in the production of aquaculture feed through extrusion processing. Ingredient blends containing different levels of SWF along with distiller dried grains, corn flour, corn gluten meal, fish meal, vitamin and mineral mix with net protein adjusted to 32% protein were formulated. The ingredient blends were extruded in …

Effects Of Bran Treatment On Rheology And Sensory Quality Of Whole Wheat Flat Bread, Jigyasha Mishra

Electronic Theses and Dissertations

The purpose of this research was to study the differences in the quality of 100% whole wheat flour (WWF) when the bran fraction was subjected to mild solvent treatment before reconstitution with the endosperm. The bran treatments included water washing and washing with ethanol solutions (50% and 100%). WWF prepared with untreated bran served as the control. Effects of these treatments on particle size, chemical composition, color, dough rheology, product formulation, and sensory characteristics of 100% whole wheat flour were studied. Grains of five HRSW cultivars (Advance, Prevail, Select, Brick and Forefront) were milled using a Quadrumat Senior mill. Gluten …

Current Status Of Falcon Populations In Saudi Arabia, Albara M. Binothman

Electronic Theses and Dissertations

Falcons (Falco spp.) are widely used for falconry in the countries of the Middle East. During the 2015 breeding season, we surveyed historic and active nest sites of Barbary (Falco pelegrinoides pelegrinoides) and Lanner (F. biarmicus) Falcons in Saudi Arabia. Field and questionnaire surveys were conducted and personal contact with falconers was made to document the current distribution and price changes for Lanner Falcons, Barbary Falcons, Saker Falcons (F. cherrug), Peregrine Falcons (F. peregrinus), Gyrfalcons (F. rusticolus), and Hybrid Falcons in Saudi Arabia. We categorized our survey into three geographic groups; southwest (A), northwest (B), and central (C) regions of …

Monitoring Global Forest Land-Use And Change, Erik J. Lindquist

Electronic Theses and Dissertations

Earth’s forests contain nearly three-fourths of the World’s floral and faunal diversity, function as a large carbon sink capable of mitigating the effects of global climate change, affect local and regional physical and chemical cycles and provide wood and non-wood products. However, humans are now capable of modifying their environment in ways more impactful and at rates faster than at any other time in history. Consistent and comparable estimates of global forest land-use and change are critical for monitoring human impacts on the Earth system. International treaties and reporting requirements aimed at safeguarding the delivery of forest-related ecosystem services depend …

Environmentally Benign Extraction Processes In Analytical Separation Of Essential Oils, John Kiratu

Electronic Theses and Dissertations

Supercritical fluid extraction (SFE) technology has been well received as an environmentally friendly processing technique. Over the last two decades, its use in many processing industries has tremendously advanced. This is as a result of pressure from regulating bodies aimed at reducing the wide-scale use of organic solvents due to negative environmental impacts. Supercritical carbon dioxide (SC-CO2) is considered to be environmentally benign and has been used in the development of a wide-range of alternative processes in various industries to totally or partially eliminate the use of organic solvents. Conventional processes for essential oil extraction involve steam distillation and organic …

Glufosinate Rate And Timing For Control Of Glyphosate-Resistant Rhizomatous Johnsongrass (Sorghum Halepense) In Glufosinate-Resistant Soybean, Randall L. Landry, Daniel O. Stephenson, Brandi C. Woolam

Faculty Publications

Field studies were conducted over a three-year period (2011, 2012, and 2013) in Louisiana to evaluate the effect of glufosinate rate and timing on glyphosate-resistant (GR) rhizomatous johnsongrass control in glufosinate-resistant soybean. Treatments included glufosinate (0.5, 0.6, or 0.7 kg ai ha-1) applied alone POST1 (46 cm tall johnsongrass) and sequentially 3 (POST2) or 4 (POST3) wk after POST1 at 0.5 or 0.6 kg ha-1. Glufosinate (0.7 kg ha-1) applied POST1 controlled johnsongrass 77% at soybean harvest. Averaged across sequential application rate, delaying the sequential application from POST2 to POST3 increased control from 65 to 78% at harvest. Increasing sequential …

Bioinformatics Investigation On The Role Of Obox Gene Family In Preimplantation Embryo Development, Wei Wang

Electronic Theses and Dissertations

Preimplantation embryo development (PED) refers to the period from fertilization to implantation in mammals. This complex process is critical for successful implantation and pregnancy. Although studies on PED have been conducted, using mainly the mouse as a model organism, the gene regulatory mechanisms are still not well understood. The next-generation sequencing technologies enable researchers to obtain the genome-wide expression profiling with single-cell resolution, which are powerful tools to study early embryogenesis. We used the single-cell RNA sequencing datasets that are available in the public domain and conducted bioinformatics analysis of expression profiling from zygote to blastocyst. Through hierarchical clustering of …

Growth Performance, Nutrient Utilization, And Metabolic Profile Of Dairy Heifers Fed Diets High In Distillers Grains With Different Forage To Concentrate Ratios, Angela Kristia Manthey

Electronic Theses and Dissertations

Two studies were conducted to evaluate the effect of limit-feeding heifers distillers grains with solubles (DDGS) with varying forage to concentrate ratios. The effects on growth, feed efficiency, rumen fermentation, nutrient digestibility, metabolic profile and onset of puberty, as well as post trial performance were investigated. First, a 16-wk feeding trial was conducted using 48 Holstein heifers to evaluate effects of dietary treatment on dry matter intake (DMI), average daily gain (ADG), growth performance, rumen fermentation, and nutrient digestibility. Treatments were 1) 30% DDGS, with the diet fed at 2.65% of body weight (BW) (30DG), 2) 40% DDGS, with the …

Fully Solution Processed Pedot:Pss And Silver Nanowire Semi-Transparent Electrodes For Thin Film Solar Cells, Bjorn Vaagensmith

Electronic Theses and Dissertations

Building integrated photovoltaics (BIPV), such as semitransparent organic solar cells (OSC) for power generating windows, is a promising method for implementing renewable energy under the looming threat of depleting fossil fuels. OSC require a solution processed transparent electrode to be cost effective; but typically employ a nonsolution processed indium tin oxide (ITO) transparent electrode. PEDOT:PSS and silver nanowire transparent electrodes have emerged as a promising alternative to ITO and are solution processed compatible. However, PEDOT:PSS requires a strong acid treatment, which is incompatible with high throughput solution processed fabrication techniques. Silver nanowires suffer from a short lifetime when subject to …