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Transcriptional Regulation Of The Endoplasmic Reticulum In Dedicated Secretory Cells, Benjamin David Moore

Arts & Sciences Graduate Student Theses and Dissertations

Differentiating cells express subsets of genes to build the cellular machinery necessary to perform their specific function as they acquire their mature fate. These subsets of genes are regulated by networks of transcription factors as cells progress through their developmental program. In secretory tissue, highly-specialized cells establish a complex secretory apparatus and scale up their cellular architecture to facilitate the production and secretion of large amounts of protein. Here, we identify a network of transcription factors responsible for the development of this cellular machinery in these professional secretory cells, and develop new tools to better study the molecular networks that …

Molecular Mechanisms Of Axon Growth And Regeneration, Dana Watt

Arts & Sciences Graduate Student Theses and Dissertations

Neurons are cells with unique and extremely polarized morphologies. The axon allows communication between the cell soma and the distantly located synaptic terminal and can extend up to one meter in humans. This exceptional cellular structure therefore has specialized biological processes dedicated to its growth, maintenance and regeneration. The structure that carries out axon elongation during development and regeneration is the growth cone, which features a cytoskeletal structure that is both highly dynamic yet consistent in overall organization. The growth cone coordinates a leading edge of dynamic actin with the microtubules of the growing axon, enabling directed outgrowth while maintaining …

Human Immunodeficiency Virus Type 1 Envelope Protein Interaction With Host Cell Coreceptor C-X-C Chemokine Receptor Type 4, Jie Zhang

Arts & Sciences Graduate Student Theses and Dissertations

Human Immunodeficiency Virus Type 1 (HIV-1) envelope protein is the sole determinant for viral entry and tropism. The ability of HIV-1 to infect susceptible host cells depends on the ability of its envelope protein to engage host cell receptor CD4 and coreceptor C-C Chemokine Receptor Type 5 (CCR5) and/or C-X-C Chemokine Receptor Type 4 (CXCR4). Most naturally occurring infections start with a single CCR5-tropic virion. In approximately 50% of HIV-1 Clade B infected patients, the viral population spontaneous develops the ability to utilize CXCR4 at a late disease stage, and this coreceptor shift corresponds to a poor prognosis for the …

Investigation Of 89zr-Siderophores As Molecular Imaging Agents For Positron Emission Tomography Imaging Of Bacterial Infections, Nora C. M. Goscinski

Arts & Sciences Graduate Student Theses and Dissertations

Siderophores are small molecules synthesized by bacteria to harvest Fe³⁺ from their environment. In infection scenarios, their production can increase infection virulence by increasing the ability of bacteria to obtain Fe³⁺ and therefore grow more rapidly. The selective uptake of siderophores in vivo in multi-bacteria environments indicates that this class of molecules has a potential use as selective imaging agents. In this work, DFO-NCS and a library of trihydroxamate siderophores were evaluated as vehicles to deliver ⁸⁹Zr selectively to bacteria for Positron Emission Tomography (PET) imaging of bacterial infections. Productive work with radiometals involves thorough knowledge of …

Ion Selectivity And Allostery In The Nak Channel, Joshua B. Brettmann

Arts & Sciences Graduate Student Theses and Dissertations

Ion channels require proper ion selectivity and regulated gating in order to perform their cellular functions. Bacterial ion channels serve as excellent model systems to study structure/function relationships concerning the fundamental processes of ion selectivity and gating. NaK, a non-selective cation channel from Bacillus cereus, has the conserved pore structure of K+ channels. However, its non-canonical selectivity filter structure leads to non-selectivity between Na+ and K+. Full selectivity is restored with two mutations that lead to the restoration of a Kcsa-like selectivity filter structure. Many mutations can be made to the selectivity filter of NaK without loss of protein stability …

Lysosomal Rnaset2 Is Required For Metabolic Stress-Mediated Ros Generation And Cell Death, George Caputa

Arts & Sciences Graduate Student Theses and Dissertations

Ectopic lipid deposition in non-adipose tissues is a hallmark of metabolic diseases, such as obesity and diabetes, and associated with cell dysfunction, cell death, and organ dysfunction. However, the molecular mechanisms linking excess free fatty acid to cell death are poorly understood. In order to identify genes critical for lipotoxicity, our lab performed a genetic screen in Chinese hamster ovary cells and isolated mutants that were resistant to lipotoxic death. In one of the lines, mutant 2B1, an allele encoding RNASET2, a ribonuclease of the T2 family, was disrupted by proviral insertion. We found that RNASET2 is induced during lipotoxicity …

Functional Consequences Of Cantu Syndrome Associated Mutations In The Atp Sensitive Potassium Channel, Paige Cooper

Arts & Sciences Graduate Student Theses and Dissertations

ATP-sensitive potassium (KATP) channels are composed of inward-rectifying potassium channel pore-forming subunits (Kir6.1 and Kir6.2, encoded by KCNJ8 and KCNJ11, respectively) and regulatory sulfonylurea receptor subunits (SUR1 and SUR2, encoded by ABCC8 and ABCC9, respectively). These channels couple metabolism to excitability in multiple tissues. Mutations in ABCC9 have been linked to Cantú syndrome (CS), a multi-organ disease characterized by congenital hypertrichosis, distinct facial features, osteochondrodysplasia, and cardiac defects. Additionally, two ABCC9 mutation-negative patients, exhibiting clinical hallmarks of CS, have been identified as having KCNJ8 mutations. This body of work is focused on determining the functional consequences and molecular mechanisms of …

Behavioral And Fmri-Based Characterization Of Cognitive Processes Supporting Learning And Retrieval Of Memory For Words In Young Adults, Binyam Nardos

Arts & Sciences Graduate Student Theses and Dissertations

A novel word is rarely defined explicitly during the first encounter. With repeated exposure, a decontextualized meaning of the word is integrated into semantic memory. With the overarching goal of characterizing the functional neuroanatomy of semantic processing in young adults, we employed a contextual word learning paradigm, creating novel synonyms for common animal/artifact nouns that, along with additional real words, served as stimuli for the lexical-decision based functional MRI (fMRI) experiment. Young adults (n=28) were given two types of word learning training administered in multiple sessions spread out over three days. The first type of training provided perceptual form-only training …

Science And Nature In The Medieval Ecological Imagination, Jessica Rezunyk

Arts & Sciences Graduate Student Theses and Dissertations

This dissertation explores the intersections between nature and culture in medieval literature and art with particular focus on Geoffrey Chaucer’s House of Fame, the thirteenth-century French Bible Moralisée (Vienna, Österreichische Nationalbibliothek Codex Vindobonensis 2554), and William Langland’s Piers Plowman. Current academic paradigms tend to place the study of nature firmly within the sciences and the study of culture firmly within the humanities, creating a gap between the fields that effectively isolates their respective methodologies and vocabularies from one another. This dissertation seeks to bridge that gap between the sciences and the humanities by approaching medieval literature through the lens of …

The Role Of Trauma And Mental Health Problems In The Perpetration Of Intimate Partner Violence In Post-Genocide Rwanda, Sarah Myers Tlapek

Arts & Sciences Graduate Student Theses and Dissertations

Exposure to political violence or war is associated with intimate partner violence (IPV) in post-conflict settings (Clark et al., 2010; Gupta, Reed, Kelly, Stein, & Williams, 2012), and civilians and veterans who develop posttraumatic stress disorder (PTSD) after exposure to combat or violence are more likely to perpetrate violence against an intimate partner (Taft, Watkins, Stafford, Street, & Monson, 2011). Rwandan health professionals estimate that after the 1994 genocide more than one-quarter of the country’s population now suffers from PTSD (Munyandamutsa, Nkubamugisha, Gex-Fabry, & Eytan, 2012). Although the majority of Rwandan women (56%) have experienced IPV in their lifetime (National …

The Mechanism And Regulation Of Mammalian Photoreceptor Dark Adaptation, Yunlu Xue

Arts & Sciences Graduate Student Theses and Dissertations

The visual perception of vertebrates begins in rod and cone photoreceptors. Both photoreceptors require visual pigments to detect light. At the first step of light detection, a chromophore molecule (i.e. 11-cis retinal), which is conjugated to the visual pigment in photoreceptor outer segment, absorbs a photon. Photoisomerization of the chromophore activates the visual pigment, triggers the phototransduction cascade, and produces electrical signals. After photoisomerization, the chromophore is ultimately converted to all-trans retinol, which must be recycled to regenerate the visual pigment. This visual pigment regeneration process is called the visual cycle. It is the rate-limiting step of the photoreceptor dark …

Solid-State Nmr Study Of The Tertiary Structure Of The Peptidoglycan Of Enterococcus Faecalis And The Structures Of Phorbol Diacetate And Bryostatin Bound To Protein Kinase Cδ C1b Domain, Hao Yang

Arts & Sciences Graduate Student Theses and Dissertations

Solid-state NMR was used to study the structures of phorbol diacetate and bryostatin bound to protein kinase Cδ C1b domain in lipid bilayers. The interaction of protein kinase C (PKC) with native physiological ligands drives fundamental cellular signal transductions, and aberrant PKC signaling is associated with cancer, cardiovascular disease, neurological disorders, stroke, pain, etc. Bryostatin modulates PKC and exhibits remarkable potential in treating cancer, Alzheimer's disease, and HIV/AIDS. However, the structural information and dynamics of this modulation, which are crucial for better understanding of PKC-ligand interactions in the membrane microenvironment to drive the development of new drugs, remain elusive. REDOR …

Antisense Rna-Mediated Epigenetic Regulation Of Brain-Derived Neurotrophic Factor., Roya Pedram Fatemi

Open Access Dissertations

Long noncoding RNAs (lncRNAs) regulate chromatin remodeling through their interactions with epigenetic enzymes during development and disease. The inhibition of the natural antisense transcript of Brain-derived neurotrophic factor (BDNF-AS), results in BDNF promoter de-repression and transcriptional upregulation, both in vitro and in vivo. Recently, we showed that BDNF-AS interacts with the histone methyltransferase enhancer of zeste homolog 2 (EZH2) to suppress BDNF mRNA and protein expression. BDNF is an important neurotrophin that is required for neural development and maintenance of the nervous system. Dysregulation of BDNF occurs in a number of neurological disorders, including: Alzheimer’s Disease, Parkinson’s Disease, Rett syndrome, …

Faculty Senate Executive Committee Agenda, December 14, 2015, Utah State University

Faculty Senate & Faculty Senate Executive Committee

• EPC Items December 2015
• Council on Teacher Education Report
• Scholarship Advisory Board
• 405.12.1 Annual Review of Faculty (First Reading)

Lrcp Initial Study, Uc Hastings College Of The Law

Long Range Campus Planning Archive

No abstract provided.

Scope Of Work, Trc

Long Range Campus Planning Archive

No abstract provided.

Curricular Affairs Committee Minutes December 14, 2015, University Of Rhode Island Faculty Senate

Faculty Senate Committee Reports and Appendices

No abstract provided.

Feeding Athens Food Drive Finale Drop Off At 3 Pm, Dec. 15 In Baker, Ohio University College Of Arts & Sciences

All Forum Articles

No abstract provided.

Board Of Directors Meeting Minutes (December 14, 2015), Dmacc

Board of Directors Meeting Minutes

This file contains the minutes from the Des Moines Area Community College Board meeting held on December 14, 2015.

Incentive Contrast As A Relative Reward, Alexandra Schmidt

Honors Projects

This study examines the relationship between rapid relative reward comparisons and incentive contrast among rats (n=5). Animals were trained to lever-press in order to obtain access to a sucrose solution (concentration used: 1%, 10% or 20% in tap water). These rewards were placed outside an operant box which could be reached through a small hole displaying sessions with mixed comparisons (1v20%, 20v1%) or single sessions (1v1%, 10v10%, 20v20%) that rotated between two spouts containing the pre-randomized order of paired blocks; allowing for comparative analysis between two spouts/concentrations and blocks of responses. Throughout weekly testing each animal experienced a …

Next-Generation Scientists Get A Taste Of Their Future Careers, Patrick M. Edwards, Linda A. George, Matthew Mctammany

Environmental Science and Management Faculty Publications and Presentations

Article provides an overview of the middle and high school poster session that took place during the 2014 Joint Aquatic Sciences Meeting (JASM) in Portland, Oregon. The poster session provided the students with opportunity to participate in the research process and prepare their posters.

Genetic And Epigenetic Variations In Asthma And Wheeze Illnesses, Todd M. Everson

Theses and Dissertations

Asthma, a chronic respiratory condition, is common worldwide with no cure and limited effective prevention strategies. It is well recognized that asthma has a multifaceted etiology, though many of the underlying mechanisms involved in asthma development, persistence and remission are still convoluted. Epigenetic mechanisms, such as DNA methylation, regulate gene-expression but are not related to changes in the actual DNA sequence. Recently, differential patterns of DNA methylation within many genes have been associated with asthma, particularly within genes involved in the differentiation of pro-inflammatory T-helper 2 (Th2) cells. DNA methylation patterns within less known biologic pathways undoubtedly are involved in …

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Newsroom, Georgia Southern University

Newsroom (2006-2023)

• Georgia Southern faculty continue excellence in 2015

University Of Northern Iowa Faculty Senate Meeting Minutes, December 14, 2015, University Of Northern Iowa. Faculty Senate.

Documents - Faculty Senate

Meeting agenda from the Faculty Senate of the University of Northern Iowa.

A Statistical Approach For Optimizing Parameters For Electrodeposition Of Indium (Iii) Sulfide Films, Potential Low-Hazard Buffer Layers For Photovoltaic Applications, Maqsood Ali Mughal

Student Theses and Dissertations

Clean and environmentally friendly technologies are centralizing industry focus towards obtaining long term solutions to many large-scale problems such as energy demand, pollution, and environmental safety. Thin film solar cell (TFSC) technology has emerged as an impressive photovoltaic (PV) technology to create clean energy from fast production lines with capabilities to reduce material usage and energy required to manufacture large area panels, hence, lowering the costs. Today, cost ($/kWh) and toxicity are the primary challenges for all PV technologies. In that respect, electrodeposited indium sulfide (In2S3) films are proposed as an alternate to hazardous cadmium sulfide (CdS) films, commonly used …

Unh Announces New Dean Of Its Manchester Campus, Erika Mantz

Media Relations

No abstract provided.

Dietary Patterns And Prostate Cancer Aggressiveness In African-American And European-American Men, Lara Ryan Schneider

Theses and Dissertations

Several foods and nutrients have been linked to prostate cancer risk, but the effect of overall diet on prostate cancer outcomes is not well understood. Previous research has primarily examined a posteriori dietary patterns in relation to prostate cancer; studies that have used a priori dietary patterns and their relationship with prostate cancer have been inconclusive. Furthermore, racial differences in prostate cancer incidence and aggressiveness are not well understood. Data from the case-only North Carolina-Louisiana Prostate Cancer Project (PCaP) was used to examine the association between overall dietary pattern, as measured by the Mediterranean Diet (MED) score and the Dietary …

Empirical Connections Between The Construct Of Value, The Quality Of Stability, And The Construct Of Regret, William Samuel Morris Jr.

Theses and Dissertations

The term value is a psychological construct frequently used in the social sciences. This research addresses the issue of stability of ratings of perceived value of participants for monetary, tangible, and intangible items that cross contexts. Also elucidated is the relationship of value to regret. Hypothesis one stated that value would be stable across time and different rating conditions. Forty-four participants rated the value of 72 stimulus items at two time points and in different contexts. Paired samples t-tests indicated 28 participants had no statistical difference in scores. Twenty-eight scores out of 44, when applied to a binomial test, indicates …

Dental Insurance As A Mitigating Factor In Reducing The Risk Of Mortality Among Working-Age Adults With Dental Caries And Periodontitis, Naveed Sadiq

Theses and Dissertations

Background Poor oral health had been associated with increased risk of having systemic diseases and thus mortality. The purpose of this research was to find if dental coverage with health insurance could mitigate the risk of death due to untreated dental caries and periodontitis among working age U.S. adults. Methods Data were drawn from NHANES III (1988-1994) and linked to mortality updated till Dec. 31st, 2011. It was restricted to those in the age range of 20-64 years adults. Furthermore, it was restricted to those who had complete data collection on all measures. Unweighted propensity scores were calculated to control …