Digital Commons Network^™

Nprl2/Tusc4 Functions As A Tumor Suppressor By Regulating Brca1’S Stability Via The E3 Ubiquitination Pathway, Yang Peng

Dissertations and Theses (Open Access)

Expression of the tumor suppressor protein BRCA1 is frequently lost in breast cancer patients, and the loss of its expression is associated with disruption of various critical functions in cells and cancer development. In the present study, we demonstrate through microarray analysis that cells with tumor suppressor candidate 4 (NPRL2/TUSC4) knockdown show critical changes to cell cycle, cell death pathways and a global impact on cancer development. More importantly, we observed a clear cluster pattern of NPRL2/TUSC4-knockdown gene profiles with established homologous recombination (HR) repair defect signature. Additionally, NPRL2/TUSC4 protein physically interacts with the E3 ligase HERC2 and prevents ubiquitin …

Change Detection In Rhesus Monkeys And Humans, Deepna T. Devkar, Deepna T. Devkar

Dissertations and Theses (Open Access)

Visual working memory (VWM) is the temporary retention of visual information and a key component of cognitive processing. The classical paradigm for studying VWM and its encoding limitations has been change detection. Early work focused on how many items could be stored in VWM, leading to the popular theory that humans could remember no more than 4±1 items. More recently, proposals have suggested that VWM is a noisy, continuous resource distributed across virtually all items in the visual field, resulting in diminished memory quality rather than limited quantity. This debate about the nature of VWM has predominantly been studied with …

Regulation Of Functional Expression Of Mechanosensitive Trpv4 Channel In The Distal Nephron By Dietary Potassium And Sodium Intake, Nabila Boukelmoune

Dissertations and Theses (Open Access)

The Ca²⁺-permeable TRPV4 channel is predominantly expressed in the distal nephron (DN) and its activity is essential for [Ca²⁺]_i elevations in response to increased tubular flow. Here, I probed the physiological mechanisms controlling TRPV4 function and expression in the DN. I found that renal TRPV4 expression and mRNA levels were significantly increased by high K⁺ diet (5%) and decreased by dietary K⁺ restriction (0.003%). In contrast, variations in Na⁺ regimen had no apparent effect on TRPV4 expression and mRNA levels. Regulation of TRPV4 protein expression by K⁺ diet was independent of …

Jab1 Negatively Regulates Pten And Promotes Resistance To Trastuzumab In Her2-Positive Breast Cancer, Thuy T. Vu

Dissertations and Theses (Open Access)

HER2-positive breast cancer, which is characterized by the over-expression of the HER2 onco-protein, accounts for approximately 20% of all breast cancer cases. Trastuzumab (Herceptin), the first targeted therapy approved for HER2-positive disease, potently prevents the activation of signaling pathways downstream of HER2 and significantly improves patients’ outcomes. However, resistance to trastuzumab is inevitable; such resistance can occur through reduced expression of PTEN protein.

Jab1 is over-expressed in 50% of primary cancers and 90% of metastatic tumors. Our lab previously showed that depletion of Jab1 in combination with trastuzumab treatment up-regulated PTEN in mouse xenografts refractory to trastuzumab. PTEN was not …

Epidermal Growth Factor Receptor Induces Fyn Expression Via Up-Regulation Of P47phox In Glioblastoma Multiforme, Blake P. Johnson

Dissertations and Theses (Open Access)

Src family kinases (SFKs) are commonly over-expressed and/or activated in glioblastoma multiforme (GBM), where they serve as key mediators of GBM cell proliferation, survival, invasion and angiogenesis. Mechanisms of allosteric SFK activation are well described; however, the SFK Fyn is commonly up-regulated at the mRNA level in multiple human cancers, including GBM, where the mode of increased expression is poorly understood. Since activating mutations in the epidermal growth factor receptor (EGFR) are commonly occurring in GBM, we examined whether EGFR could induce Fyn expression. Here, we found that wild-type EGFR, and to a greater extent hyper-activating EGFR mutants, EGFRΔIII and …

Targeting Cox-2 And Rank In Aggressive Breast Cancers: Inflammatory Breast Cancer And Triple-Negative Breast Cancer, Monica Elizabeth Reyes

Dissertations and Theses (Open Access)

Inflammatory breast cancer (IBC) and triple-negative breast cancer (TNBC) are two highly aggressive breast cancer subtypes associated with a poor outcome. Despite sensitivity to current treatment, these breast cancers subtypes have a high recurrence rate and proclivity to metastasize early. The aggressiveness of IBC and TNBC have been linked to CSCs and epithelial to mesenchymal transition (EMT), which are critical features of breast cancer progression and metastasis. The clinical challenge faced in the treatment of IBC and TNBC is finding a treatment strategy to target the cancer stem-like (CSC) population to block metastasis. Cyclooxygenase-2 (COX-2) and receptor activator of nuclear …

Dual Assembly Nanoparticles Achieve Sustained Silencing Of Znf304: A Novel Transcription Factor For Β1 Integrin, Burcu Aslan

Dissertations and Theses (Open Access)

Ovarian cancer (OC) is a highly metastatic disease, but no effective strategies to this process currently are known. Here, an integrated computational analysis of The Cancer Genome Atlas ovarian cancer dataset coupled with experimental validation identified a novel zinc finger transcriptional factor 304 (ZNF304) as one of the key factors for ovarian cancer metastasis. High tumoral ZNF304 expression was associated with poor overall survival in ovarian cancer patients. Through reverse phase protein array analysis, we demonstrated that ZNF304 promotes multiple proto-oncogenic pathways important for cell survival, migration, and invasion. ZNF304 transcriptionally regulates β1 integrin, which subsequently regulates Src/focal adhesion kinase …

Targeting Cox-2 And Rank In Aggressive Breast Cancers: Inflammatory Breast Cancer And Triple-Negative Breast Cancer, Monica E. Reyes

Dissertations and Theses (Open Access)

Inflammatory breast cancer (IBC) and triple-negative breast cancer (TNBC) are two highly aggressive breast cancer subtypes associated with a poor outcome. Despite sensitivity to current treatment, these breast cancers subtypes have a high recurrence rate and proclivity to metastasize early. The aggressiveness of IBC and TNBC have been linked to CSCs and epithelial to mesenchymal transition (EMT), which are critical features of breast cancer progression and metastasis. The clinical challenge faced in the treatment of IBC and TNBC is finding a treatment strategy to target the cancer stem-like (CSC) population to block metastasis. Cyclooxygenase-2 (COX-2) and receptor activator of nuclear …

A Study Of The Regularity Of Autocorrelation Of Manipulation Of Wrist Motion During Eating, Xueting Yu

All Theses

This thesis considers the problem of detecting the eating activities (e.g. meals, snacks) of people by tracking their wrist motion. Our goal is to automatically de- tect the start and end times of eating activities during free-living. It builds upon previous work done by our research group [6] [7] [8] [21]. The detection is done by segmenting data into segments then classifying the segments as EA (eating activity) and NonEA (non-eating activity) using a Bayesian classier. Previous features used in the classier developed by our group included the sum of acceleration, the amount of manipulation, the amount of wrist roll …

The Effects Of Reminder Distinctiveness And Anticipatory Interval On Prospective Memory, Natalee Baldwin

All Theses

Prospective memory failures (or failures to remember a future intention) can result in a wide range of negative consequences. The use of reminders has been shown to improve the rate of PM successes. The aim of the current study was to examine the effectiveness of reminders based on their type (text or picture) and their timing. We hypothesized that successful PM performance would be successfully maintained over longer anticipatory intervals when paired with picture reminders rather than with simple text reminders because of the inherent distinctiveness of pictures. We also expected that performance for younger adults would be better than …

Prevalence Of Human Noroviruses In Commercial Retail Food Establishment Without A Recently Reported Outbreak Of Gastroenteritis, Chaoyi Tang

All Theses

Human noroviruses (NoV) are a leading cause of acute gastroenteritis, causing nearly 58% of foodborne disease in the USA. The aim of our study was to determine the prevalence of NoV on select bathroom surfaces in food establishments in South Carolina (SC). All SC counties (N=46) were sorted by population density into high-, medium-, and low-density categories. Three counties were randomly selected from each population category. Lists of all foodservice establishments in each selected county (N=9) were obtained from the Department of Health and Environmental Control and a total of 120 foodservice establishments were randomly selected. Swabs were collected from …

Do I Have Enough Money? An Examination Of The Roles Of Income And Income Perceptions On Nursing Turnover Intentions, Janelle Cheung

All Theses

Economic stress is an understudied, but potentially critical, concern that deserves more attention in the literature because it has important implications for employees and organizations. The present study sought to bring researchers and practitioners' attention to this area of research by examining the impact of income and income perceptions on turnover intentions. Very few published studies have investigated the mechanisms underlying the relationship between income and turnover intentions. As a novel contribution, the present study examined how perceived adequacy of current and future income each and simultaneously mediated the relationship between income and turnover intentions. Further, the study tested whether …

The Effects Of Probiotics Supplementation On Health Using Caenorhabditis Elegans As A Model System, Miranda Klees

All Theses

The 'Western Diet,' prominent among developed nations, often refers to a diet rich in meat proteins and refined sugars. Western society may in part be plagued with obesity and obesity related diseases due to a diet enriched with glucose. With an increasing glycemic index observed in Western society, it comes to no surprise that obesity, diabetes, heart disease, and other obesity related diseases are on the rise. The Centers for Disease Control and Prevention describes obesity as an epidemic, affecting more than 35% of U.S. citizens. Shortened lifespan and increased susceptibility to pathogens are associated with these diseases and linked …

Charitable Donations: An Analysis Of The Differences In Donation Patterns By Income Level, Elizabeth Ficklin

All Theses

This thesis examines the possibility of grouping charitable donors by income level to develop a set of models that can more accurately predict charitable donations. Previous work is inconsistent in predicting charitable donations. This work helps to determine if these inconsistencies are a result of methodological differences between researchers, or if group membership is an important factor in predicting charitable donations as suggested by some researchers. This research only found four variables that were common to all three income groups, frequency of church attendance, family income, age, and years of education. Results show that additional variables can serve as predictors …

Using Gis To Prioritize Green Infrastructure Installation Strategies In An Urban Watershed, Lauren Owen

All Theses

This study seeks to quantify runoff volume generation and peak flow rates from the urban Sand River Headwaters to determine the most effective placement of additional green infrastructure in Aiken, SC. ArcMap 10.1, HEC-GeoHMS, and HEC-HMS were used to delineate a total outlet watershed along with subwatershed(s) for urban stormwater infrastructure system by 'burning' the stormwater system at an artificial elevation below the existing topologically-based Digital Elevation Model (DEM). The result was a higher resolution DEM that allowed for storm routing and subsequent volume and flow predictions compared to that based on the original DEM created by using Light Detecting …

Stochastic Wavenumber Estimation: Damage Detection Through Simulated Guide Lamb Waves, Garrison Stevens

All Theses

Structural Health Monitoring (SHM) is a field of research that seeks to identify the onset of damage in infrastructure systems such that catastrophic failures can be averted. Acoustic Wavenumber Spectroscopy (AWS) is an emerging SHM technique that can identify and locate change of thickness resulting from damage in thin plates through the estimation of the characteristic wavenumber. AWS measures the ultrasonically induced vibrations of thin plates by laser scanners and monitors the propagation of Rayleigh-Lamb waves through the structure. This method is particularly suitable for thin-walled structures such as those contained in pipes, airplanes, and wind turbine blades. While AWS …

Fabrication And Characterization Of Magnetic Nanoparticle Composite Membranes, Akeem Cruickshank

All Theses

To effectively and accurately deliver drugs within the human body, both new designs and components for implantable micropumps are being studied. Designs must ensure high biocompatibility, drug compatibility, accuracy and small power consumption. The focus of this thesis was to fabricate a prototype magnetic nanoparticle membrane for eventual incorporation into a biomedical pump and then determine the relationship between this membrane deflection and applied pneumatic or magnetic force. The magnetic nanoparticle polymer composite (MNPC) membranes in this study were composed of crosslinked polydimethylsiloxane (PDMS) and iron oxide nanoparticles (IONPs). An optimal iron oxide fabrication route was identified and particle size …

Inhibition Of Vibrio Cholerae Biofilm Formation By Water-Soluble Cranberry Extracts, Daniel Pederson

All Theses

Vibrio cholerae, a Gram-negative bacterium found in natural aquatic environments, is the causative agent of cholera, a severe diarrheal disease most commonly spread through drinking water. An essential component to this pathogen's success and persistence in the environment is its ability to attach to both biotic and abiotic surfaces via biofilm formation. Biofilms not only aid in surface attachment, but also provide a barrier that protects and enhances survival. Water-soluble extracts from the North American cranberry (Vaccinium macrocarpon) were found to dramatically inhibit V. cholerae biofilm formation at a low concentration of 2mg/ml. This inhibition was not due to acidification …

Characterizing The Effect Of Vegetable Oil-Based Electron Donor Concentration On Complete Dechlorination Of Trichloroethene And Vinyl Chloride, Alex Haluska

All Theses

Chlorinated ethenes are known or suspected carcinogens. They are also among the most frequently detected organic groundwater contaminants because of wide use and poor disposal practices. Within anaerobic aquifer systems, Fe(III) reduction is a prevalent terminal electron accepting process and has often been reported as a competitive electron acceptor with respect to trichloroethene (TCE), cis-dichloroethene (cis-DCE), and vinyl chloride (VC) reduction. Often, practitioners add excessive amounts of electron donor to compensate for this competition in an effort to avoid incomplete dechlorination or "cis-DCE and VC stall." Contaminated aquifer material incubations were setup to assess dechlorination rates and extent under Fe(III)-reducing …

Teachers' Making Meaning Of Reduced Class Sizes: A Phenomenology, Brenda Ludy Snow

Dissertations

It is every teacher’s dream to have a classroom full of students who come to class each and every day highly motivated to learn. However, in all reality teachers know that this is not always the case. Researchers continue to search for reasonable alternatives to improve students’ learning. As children enter school a good start is imperative if they are to succeed in school, (Achilles & Finn, 2000). Class size reduction is a strong alternative and should be considered as part of a reasonable plan for student learning.

For this qualitative study, I examined and described the experiences of 12 …

Steps Toward A Net-Zero Campus With Renewable Energy Resources, Andrew Clarke

All Dissertations

With the increasing attention and support behind plug in hybrid electric vehicles, research must be conducted to examine the impacts of vehicles on electric distribution and transmission systems. This research aims first to model the behavior of vehicle battery chargers during system disturbances and mitigate any impacts. A distribution test system example is modeled and several different vehicle charger topologies are added. Faults are applied to the distribution system with vehicle chargers connected and the results are examined. Based on these results, a control strategy to mitigate their negative impacts is suggested. Photovoltaic panels are then added to the system …

Development Of A Translational, Tissue-Engineered Approach To Replacement Of Myocardial Infarct Scar Tissue, Jason Schulte

All Dissertations

Myocardial infarction (MI) affects nearly 600,000 individuals each year, and the resulting damage initiates a pathophysiological progression towards congestive heart failure (CHF). A shortage of donor organs precludes heart transplantation as a practical solution, and neither surgical intervention nor stem cell therapy have yielded consistent and sufficiently positive results in clinical investigation. To prevent CHF, prospective therapies must target the cause of the maladaptive cardiac remodeling which precedes it--the nonfunctional, dyskinetic infarct scar--and aim to replace it with functional cardiac muscle. Tissue engineering holds promise for the development of novel therapies to either halt or reverse post-MI cardiac remodeling. However, …

Targeted Delivery Of Anti-Inflammatory And Anti-Oxidant Drugs For Treatment Of Chronic Obstructive Pulmonary Diseases, Yun Xiang

All Dissertations

Chronic obstructive pulmonary disease (COPD) was found to be the fourth leading cause of death in 2004, but current treatments can only relieve the symptoms and improve the quality of life for the patients. Glucocorticoids have been broadly applied in other inflammatory conditions with promising therapeutic effects, but performed poorly in COPD. The phenomenon called glucocorticoid resistance is responsible for glucocorticoid insensitivity in COPD. Researchers have proven that elevated oxidative stress in the lung of a COPD patient is one of the main causes of blocking the function of glucocorticoids. We hypothesize that reducing the oxidative stress in the lung …

Empowering Citizens In A Global Era: A Grounded Theory Study Of Community Gardens, Anita Tam

All Dissertations

In our global era, the modern food system can be viewed as consisting of a dominant macro-level corporate food industry, challenged by broad-based meso-level food justice and democracy movements, which are in turn fueled by micro-level community food initiatives. Research has yet to examine the role of community gardens in the context of this complex, multi-level food system. Grounded theory methodology was thus used to explore the deeper meaning of the community gardening experience to participants, in order to better understand the ways in which community gardens may scale up and contribute to democratizing the food system. Analysis of the …

Design With Emotion: Improving Web Search Experience For Older Adults, Tamirat Abegaz

All Dissertations

Research indicates that older adults search for information all together about 15% less than younger adults prior to making decisions. Prior research findings associated such behavior mainly with age-related cognitive difficulties. However, recent studies indicate that emotion is linked to influence search decision quality. This research approaches questions about why older adults search less and how this search behavior could be improved. The research is motivated by the broader issues of older users' search behavior, while focusing on the emotional usability of search engine user interfaces. Therefore, this research attempts to accomplish the following three objectives: a) to explore the …

Autonomous Control And Automotive Simulator Based Driver Training Methodologies For Vehicle Run-Off-Road And Recovery Events, Paul Freeman

All Dissertations

Traffic fatalities and injuries continue to demand the attention of researchers and governments across the world as they remain significant factors in public health and safety. Enhanced legislature along with vehicle and roadway technology has helped to reduce the impact of traffic crashes in many scenarios. However, one specifically troublesome area of traffic safety, which persists, is run-off-road (ROR) where a vehicle's wheels leave the paved portion of the roadway and begin traveling on the shoulder or side of the road. Large percentages of fatal and injury traffic crashes are attributable to ROR. One of the most critical reasons why …

Development And Application Of Scalable Two-Dimensional High Performance Liquid Chromatography For Aromatic Selective Separations Of Biologically Active Phytochemicals From Oplopanax Horridus, Gregory Jones

All Dissertations

The high demand for more efficient purification processes with increased automation and throughput pushes the development of more advanced preparative, pilot, and process scale HPLC instrumentation that is capable of achieving higher purities in a shorter amount of time than are currently achieved using one dimensional separations. A preparative scale 2D HPLC system was designed and reduced to practice in order to demonstrate the capacity for scalability of on-line comprehensive 2D HPLC separations of basic compounds from a challenging natural product extract of Oplopanax horridus. The methodology and instrumentation design herein permits direct method transfer from analytical to preparative scale …

Essays In Public And Corporate Finance, Joseph Newhard

All Dissertations

Each of the three chapters of this dissertation makes a unique contribution to the fields of public finance or corporate finance. In chapter one I show that tax-price is increasing in workplace risk due to a positive wage-risk response that is observed in the labor supply price for hazardous industries. This result implies that, holding human capital constant, workers in more dangerous industries will demand a relatively smaller public sector. I test this with county-level data on fatality rates and support for the two major party candidates in the 2004 US Presidential election. Taking Republicans to represent the party of …

Particle Scavenging By Water Drops In An Ultrasonic Standing Wave Field, Weiyu Ran

All Dissertations

Currently the backbone of the world's energy supply is composed of fossil fuels. However, the combustion of fossil fuels results in the production of enormous quantities of particulate pollutants. The smog resulting from these particulate pollutants causes significant health problem for city dwellers. Wet scrubbers, which use a water spray to scavenge airborne particles, is one of the most widely used devices to control particulate pollutants. Typical wet scrubbers can scavenge particles with diameters bigger than 10 um, but it is inefficient in scavenging particles with diameters on the order of 1 um. Unfortunately these fine particles are more dangerous …

Rebuilding Social Organization In Low-Income Neighborhoods: The Influence Of Habitat For Humanity, John Lattimore

All Dissertations

Research has linked neighborhoods of concentrated poverty with high crime, low employment, poor health, and low educational achievement. Because of these linkages, federal housing policy over the past few decades has often tried to 'deconcentrate' or disperse the poor from these neighborhoods into more affluent neighborhoods with the hope that better institutions and better neighbors will motivate these families to improve their lives. However research on large mobility programs such as Gautreaux and Moving to Opportunity (MTO), has found mostly mixed results and criticized the programs for having a small impact. Race and income have also proven to be significant …