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Starvation-Associated Genome Restructuring Can Lead To Reproductive Isolation In Yeast, Evgueny Kroll, Scott Coyle, Barbara Dunn, Gregory Koniges, Anthony Aragon, Jeremy Edwards, Frank Rosenzweig

Biological Sciences Faculty Publications

Knowledge of the mechanisms that lead to reproductive isolation is essential for understanding population structure and speciation. While several models have been advanced to explain post-mating reproductive isolation, experimental data supporting most are indirect. Laboratory investigations of this phenomenon are typically carried out under benign conditions, which result in low rates of genetic change unlikely to initiate reproductive isolation. Previously, we described an experimental system using the yeast Saccharomyces cerevisiae where starvation served as a proxy to any stress that decreases reproduction and/or survivorship. We showed that novel lineages with restructured genomes quickly emerged in starved populations, and that these …

Participant/Observer Vol. 6. No. 1 Summer 2013, Anthropology Department

Participant/Observer

A Newsletter of the friends of the Western Michigan University Department of Anthropology

Table of Contents:

• Welcome from the Editor
• Recent Investigations of Fort St. Joseph
• 2012 Finger Lakes National forest Farmstead Archaeology Project Season
• Michigan Archaeology Day
• Liberty Hyde Bailey Museum Project in South Haven
• Where Are Our Alumni Now?
• Faculty Updates
• Annual Speaker Series
• WMU at SHA
• Recognize anyone?
• 2012-13 Department of Anthropology Awards

Ua64/2 The Challenge: Magazine For The Center For Gifted Studies (No. 31, Summer 2013), Center For Gifted Studies, Tracy Inman Editor

Gifted Studies Publications

No abstract provided.

A Practical And Sensitive Method Of Quantitating Lymphangiogenesis In Vivo, Mousumi Majumder, Xiping Xin, Peeyush K. Lala

Paediatrics Publications

To address the inadequacy of current assays, we developed a directed in vivo lymphangiogenesis assay (DIVLA) by modifying an established directed in vivo angiogenesis assay. Silicon tubes (angioreactors) were implanted in the dorsal flanks of nude mice. Tubes contained either growth factor-reduced basement membrane extract (BME)-alone (negative control) or BME-containing vascular endothelial growth factor (VEGF)-D (positive control for lymphangiogenesis) or FGF-2/VEGF-A (positive control for angiogenesis) or a high VEGF-D-expressing breast cancer cell line MDA-MD-468LN (468-LN), or VEGF-D-silenced 468LN. Lymphangiogenesis was detected superficially with Evans Blue dye tracing and measured in the cellular contents of angioreactors by multiple approaches: lymphatic vessel …

Research View, Summer 2013

Research View

Contents of this issue include:

The Air We Breathe: UM researcher finds young brains harmed by urban air pollution

Quick Looks: Study of Mealybug Bacteria Reveals Secrets of Symbiosis -- Award to Study Hospital Discharge Process in Rural Areas -- Autism Waiver Program Shows Promising Results -- Paper Details Stressors Faced by Sexual Minority Vets -- Innovation and Imagination: Events Highlight Research and Scholarship

There's an App for That: Researchers develop modern tool to measure heat stress in humans

Warring With Words: Religion scholar chases text destruction in the ancient world

Issue No. 96: July 2013, Historical Society Of New Mexico

La Crónica de Nuevo México

1 Las Cruces Inundated by Flash Flooding, September 1875
1 "The Chosen People"
2 Membership Benefits Historical Society of New Mexico
3 Bosque del Apache National Wildlife Refuge
4 New Mexico Boys State 1953
4 Longtime Curator Receives State Award
4 Book Review, New Mexico Art through Time: Prehistory to the Present
4 In Memoriam
5 2013 Historical Society of New Mexico Awards Ceremony Held on Saturday, April 20, Las Cruces Convention Center
5 Arizona History Convention 2013 Awards Ceremony Held on Saturday, April 20, Las Cruces Convention Center
7 Sunnyslope, Arizona, Images of America Series
7 About the Author …

Savvy Seed Sorter Gains New Fans, Thomas C. Pearson, Marcia Wood

Agricultural Research Magazine

Simple, swift, and comparatively inexpensive, a color-image-based seed sorter is helping plant breeders and others separate the seeds they want from those they don’t—with an impressive degree of accuracy.

Agricultural Research Service agricultural engineer Thomas C. Pearson, based at the agency’s Center for Grain and Animal Health Research in Manhattan, Kansas, developed the sorter in collaboration with National Manufacturing in Lincoln, Nebraska. The company has marketed the device to customers in the United States and abroad since 2010.

In tests, the compact, portable sorter— a simpler and faster version of other machine-vision equipment that Pearson developed in 2009—speedily differentiated kernels …

High-Throughput Analysis Of Antimalarial Susceptibility Data By The Worldwide Antimalarial Resistance Network (Wwarn) In Vitro Analysis And Reporting Tool, Charles J. Woodrow, Sabina Dahlström, Richard Cooksey, Jennifer A. Flegg, Hervé Le Nagard, France Mentré, Claribel Murillo, Didier Ménard, François Nosten, Kanlaya Sriprawat, Lise Musset, Neils B. Quashie, Pharath Lim, Rick M. Fairhurst, Sam L. Nsobya, Veronique Sinou, Harald Noedl, Bruno Pradines, Jacob D. Johnson, Philippe J. Guerin, Carol H. Sibley, Jacques Le Bras

United States Army: Publications

Assessment of in vitro susceptibility is a fundamental component of antimalarial surveillance studies, but wide variations in the measurement of parasite growth and the calculation of inhibitory constants make comparisons of data from different laboratories difficult. Here we describe a Web-based, high-throughput in vitro analysis and reporting tool (IVART) generating inhibitory constants for large data sets. Fourteen primary data sets examining laboratory-determined susceptibility to artemisinin derivatives and artemisinin combination therapy partner drugs were collated from 11 laboratories. Drug concentrations associated with half-maximal inhibition of growth (IC₅₀s) were determined by a modified sigmoid E_max model-fitting algorithm, allowing standardized …

The Measurement Of The Dielectric Constant Of Concrete Pipes And Clay Pipes, David Mcgraw

Doctoral Dissertations

To optimize the effectiveness of the rehabilitation of underground utilities, taking in consideration limitation of available resources, there is a need for a cost effective and efficient sensing systems capable of providing effective, in real time and in situ, measurement of infrastructural characteristics. To carry out accurate non-destructive condition assessment of buried and above ground infrastructure such as sewers, bridges, pavements and dams, an advanced ultra-wideband (UWB) based radar was developed at Trenchless Technology Centre (TTC) and Centre for Applied Physics Studies (CAPS) at Louisiana Tech University (LTU). One of the major issues in designing the FCC compliant UWB radar …

Air Pollution: The Trees Aren't Lichen It, Wes L. Hanson, Leigh A. Hanson, Meghan Lockwood, Morgan Yarber

2013 Projects

The Keck Summer Collaborative Research Program provides opportunities for Linfield College students and faculty to conduct research on issues related to the Pacific Northwest, and to bring the research findings back into the classroom within the subsequent academic year. Students partner with faculty to conduct research and present their work to other students, Linfield staff and faculty, and community members during a series of brown bag lunches. Wes Hanson, Leigh Hanson, Meghan Lockwood, and Morgan Yarber conducted research with Nancy Broshot and gave this presentation during the summer of 2013.

Circulation, Vol. 18, No. 3, Center For Coastal Physical Oceanography, Old Dominion University, Tal Ezer, Larry P. Atkinson

CCPO Circulation

Summer 2013 issue of CCPO Circulation featuring article "The Connection Between Local Sea Level Rise, Climate Change and Ocean Circulation" by Tal Ezer and Larry P. Atkinson

Old Dominion University Climate Change And Sea Level Rise Initiative, Summer 2013, Larry P. Atkinson (Editor), Hans-Peter Plag (Editor)

CCSLRI Newsletters

Summer 2013 Newsletter of the Old Dominion University Climate Change and Sea Level Rise Initiative

On Point 2013 Summer, Longwood University

Alumni Newsletters & Bulletins

No abstract provided.

Exploration Of Aqueous Interfaces And Their Effect On Ion Behavior, Oneka T. Cummings

Doctoral Dissertations

An in-depth understanding of a wide range of physical, chemical, atmospheric and biological processes can only be achieved after the structure and dynamics of interfaces and the interfacial behavior of aqueous species, such as ions, are thoroughly studied and understood. This dissertation describes computational studies conducted to gain a more comprehensive understanding of such interfaces and the behavior of ions in the bulk and interfacial regions of the (1) air/water interface, and (2) alkane/water interfaces.

At the air/water interface the effect of counterion (sodium cations) charge and the influence of ion pairing on anion (chloride) propensity for the air/water interface …

The Search, The Jackson Laboratory

Search Magazine

No abstract provided.

2012-2013 - Graduate Council Annual Report, College Of Graduate And Continuing Studies, East Tennessee State University

Graduate Council Agendas and Minutes

No abstract provided.

Elucidating Internucleosome Interactions And The Roles Of Histone Tails, Steven C. Howell, Kurt Andresen, Isabel Jimenez-Useche, Chongli Yuan, Xiangyun Qiu

Physics and Astronomy Faculty Publications

The nucleosome is the first level of genome organization and regulation in eukaryotes where negatively charged DNA is wrapped around largely positively charged histone proteins. Interaction between nucleosomes is dominated by electrostatics at long range and guided by specific contacts at short range, particularly involving their flexible histone tails. We have thus quantified how internucleosome interactions are modulated by salts (KCl, MgCl₂) and histone tail deletions (H3, H4 N-terminal), using small-angle x-ray scattering and theoretical modeling. We found that measured effective charges at low salts are ∼1/5th of the theoretically predicted renormalized charges and that H4 tail deletion …

Occupational Exposures To Respirable Crystalline Silica During Hydraulic Fracturing, Eric J. Esswein, Michael Breitenstein, John Snawder, Max Kiefer, W. Karl Sieber

United States Public Health Resources

This report describes a previously uncharacterized occupational health hazard: work crew exposures to respirable crystalline silica during hydraulic fracturing. Hydraulic fracturing involves high pressure injection of large volumes of water and sand, and smaller quantities of well treatment chemicals, into a gas or oil well to fracture shale or other rock formations, allowing more efficient recovery of hydrocarbons from a petroleum-bearing reservoir. Crystalline silica (“frac sand”) is commonly used as a proppant to hold open cracks and fissures created by hydraulic pressure. Each stage of the process requires hundreds of thousands of pounds of quartz containing sand; millions of pounds …

National And Regional Estimates Of Term And Preterm Babies Born Small For Gestational Age In 138 Low-Income And Middle-Income Countries In 2010, Anne C.C. Lee, Joanne D. Katz, Hannah Blencowe, Simon Cousens, Naoko Kozuki, James M. Tielsch, +30 Additional Authors

Global Health Faculty Publications

Background

National estimates for the numbers of babies born small for gestational age and the comorbidity with preterm birth are unavailable. We aimed to estimate the prevalence of term and preterm babies born small for gestational age (term-SGA and preterm-SGA), and the relation to low birthweight (<2500 >g), in 138 countries of low and middle income in 2010.

Methods

Small for gestational age was defined as lower than the 10th centile for fetal growth from the 1991 US national reference population. Data from 22 birth cohort studies (14 low-income and middle-income countries) and from the WHO Global Survey on Maternal …

Wooster Magazine: Summer 2013, Karol Crosbie

Wooster Magazine: 2011-2020

This edition of the Wooster Magazine was published in the summer of 2013. The edition begins with an article by alum David Gilliss '80 reflecting on the Wooster Network and Independent Study. Page four features an article by President Grant Cornwell about Wooster's slogan, "America's premier college for mentored undergraduate research." A section by Karol Crosbie looks at the Scot Center and its fitness program. Both the women's and men's Ultimate Frisbee teams are highlighted in a story regarding their history and present. An advertisement for Alumni Weekend is on page six and seven. Some seniors have their independent studies …

Giant Cell Tumour Of The Sacrum: Function-Preserving Surgery With Extended Curettage And Ilio-Lumbar Fusion, Riaz Hussain Lakhdawala, Tashfeen Ahmad, Ather Enam

Section of Orthopaedic Surgery

Giant cell tumours of the sacrum pose a unique therapeutic challenge due to the inaccessibility of the tumour, significant intra-operative blood loss from extensive vascularity, high rate of local recurrence with conservative surgery, and loss of neurological function and mechanical instability with en-bloc excision. We present a case where successful outcome was achieved by tailoring treatment in consideration of the above issues. A 28 year old male diagnosed on biopsy to have giant cell tumour of the sacrum presented to us with low-back pain, left-sided S1 radiculopathy, ankle weakness and urinary incontinence. MRI showed a tumour involving the S1 and …

Introduction: Wounds Of War: Meeting The Needs Of Active-Duty Military Personnel And Veterans With Post-Traumatic Stress Disorder, Olympia Duhart, Kathy L. Cerminara

Faculty Scholarship

No abstract provided.

Repairing The Antibiotic Pipeline: Can The Gain Act Do It?, Caitlin Forsyth

Washington Journal of Law, Technology & Arts

Antibiotic resistance, according to the World Health Organization, is one of the greatest threats to public health. To combat the problem, new antibiotics need to be developed. However, antibiotic research and development is fraught with scientific and economic problems. Recognizing these problems and the public health threat posed by antibiotic resistance, Congress passed the GAIN Act, which President Obama signed into law in June 2012. The GAIN Act (Act) incentivizes pharmaceutical companies to invest in antibiotic research and development. This Article will outline the incentives in the Act and suggest why the Act may not solve the growing antibiotic resistance …

Glad To Be Sad, And Other Examples Of Benign Masochism, Paul Rozin, Lily Guillot, Katrina Fincher, Alexander Rozin, Eli Tsukayama

Music Theory, History & Composition Faculty Publications & Performances

No abstract provided.

A Way Of Seeing: The Transformation Of American Soldiers' Snapshot Photography During The Vietnam War, Sara Hagerty

Art & Art History ETDs

Cultural conventions of appropriateness, social standards of artistic practice, and contemporary vernacular traditions of how to visually commemorate ones life impact snapshot photography. An analysis of American soldiers' snapshots taken during the Vietnam War from the National Veterans Art Museum's archive in Chicago reveals a unique set of images due to their origins as snapshots and their stylistic references to other visual frameworks that are historically used to represent war. Through an examination of the culture of snapshot making, the historic presence of cameras on the battlefield, the conditions faced by American soldiers during the Vietnam War, and the popular …

Immunomodulatory Activity Of Sambucus Mexicana And Trichostema Lanatum On Lps Stimulated Raw 264.7 Macrophage Cells, Victoria Hester, P. Matthew Joyner

Featured Research

Chumash medicinal plants Sambucus mexicana (Mexican elderberry) and Trichostema lanatum (woolly blue curls) were tested for immunomodulatory activity. Anti-inflammatory effects were determined by treating LPS induced RAW 264.7 macrophage cells with plant extracts and measuring the levels of cytokines: tumor necrosis factor alpha (TNF-alpha) and interleukin 10 (IL-10). We hypothesized that both plants would exert immunomodulatory activity by reducing the pro-inflammatory production of TNF-alpha or by promoting M2 polarization with a concurrent increase in IL-10 production. At concentration 0.01 mg/mL woolly blue curls and Mexican elderberry demonstrated anti-inflammatory activity by reducing the concentration of TNF-alpha in vitro, while levels of …

The Survivin And Ciap1 Anti-Apoptotic Proteins Are Differentially Downregulated In Response To Endoplasmic Reticulum Stress, Vicki Mercado, Jay L. Brewster

Featured Research

The endoplasmic reticulum (ER) is an organelle tasked with synthesis and transport of 50% of new cellular proteins. Dysfunction within this organelle creates signals for repair, adaptation, and in severe cases, cellular apoptosis. Multiple human diseases have been associated with ER dysfunction, and the activation of apoptosis in important populations of cells. Inhibitor of Apoptosis (IAP) proteins are cytosolic proteins that play an anti-apoptotic role in the cytosol. The relationship between endoplasmic reticulum (ER) stress and the expression/stability of IAPs is not well characterized. The objective of this study was to characterize the affect of ER stress on the expression/stability …

Variation In Mechanical Strength Of Ferns In The Santa Monica And Santa Cruz Mountains, Helen I. Holmlund

Featured Research

Mentor: Stephen D. Davis

In recent years, experts in plant physiology have begun to explore the functional traits of ferns, especially in regards to their tissue-water relations. However, to our knowledge, no scientist had yet examined the relationship between fern biomechanics and physiology. We examined the mechanical properties of fern stipes (stems) and attempted to relate those properties to ecological and physiological traits. Based on our knowledge of fern anatomy, we hypothesized that stipe mechanical strength would not correlate with cavitation resistance as it does in seed-bearing plants. This assertion that mechanical strength will not relate to cavitation resistance begs …

The Regulatory Effect Of Semaphorin 7a On Proliferation And Migration In Human Umbilical Vein Endothelial Cells, Steven R. Flemming, Donna Nofziger-Plank

Featured Research

Semaphorin 7A (SEMA 7A), a factor originally identified as regulating axon growth, has recently been implicated as a pro-angiogenic factor. The molecular mechanisms for this ability to stimulate angiogenesis have not been identified. This study examines if SEMA 7A can have a direct effect on vascular endothelial cells or whether it indirectly induces angiogenesis through stimulation and recruitment of macrophages as has been suggested. Using a human umbilical vein endothelial cells (HUVECs), the ability of SEMA 7A to affect proliferation and migration was examined. HUVECs were exposed to SEMA 7A directly or to conditioned media collected from macrophages exposed to …

Effect Of Shear Stress On Ecnos Expression And Dilation In Soleus Feed Arteries, Blanca Perez, Jay L. Brewster, Jeffrey Jasperse

Featured Research

Shear stress causes artery dilation and increased expression of endothelial cell nitric oxide synthase (ecNOS) in coronary and placental arteries. We sought to determine the importance of shear stress in maintaining normal dilation and normal levels of ecNOS in rat soleus feed arteries (SFA). SFA were isolated from male Sprague-Dawley rats and cannulated for in vitro microscopy (Fig. 6). SFA were exposed to no shear stress, low shear stress, or high shear stress conditions for 4 hours. After 4 hours, endothelium-dependent dilation (acetylcholine: ACh) and endothelium-independent dilation (sodium nitroprusside: SNP) were tested. Arteries were then uncannulated, mRNA was isolated, and …