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Biological Characterization Of 3-(2-Amino-Ethyl)-5-[3-(4-Butoxyl-Phenyl)-Propylidene]-Thiazolidine-2,4-Dione (K145) As A Selective Sphingosine Kinase-2 Inhibitor And Anticancer Agent, Kai Liu, Tai L. Guo, Nitai C. Hait, Jeremy Allegood, Hardik I. Parikh, Wenfang Xu, Glen E. Kellogg, Steven Grant, Sarah Spiegel, Shijun Zhang

Medicinal Chemistry Publications

In our effort to develop selective sphingosine kinase-2 (SphK2) inhibitors as pharmacological tools, a thiazolidine-2,4-dione analogue, 3-(2-amino-ethyl)-5-[3-(4-butoxyl-phenyl)-propylidene]-thiazolidine-2,4-dione(K145), was synthesized and biologically characterized. Biochemical assay results indicate that K145 is a selective SphK2 inhibitor. Molecular modeling studies also support this notion. In vitro studies using human leukemia U937 cells demonstrated that K145 accumulates in U937 cells, suppresses the S1P level, and inhibits SphK2. K145 also exhibited inhibitory effects on the growth of U937 cells as well as apoptotic effects in U937 cells, and that these effects may be through the inhibition of down-stream ERK and Akt signaling pathways. K145 also significantly …

A Science Of Context: The Qualitative Approach As Fundamental To Strategic Thought, Matthew J. Schmidt

Political Science Faculty Publications

(from book introduction, pp. 6-7) "Schmidt describes the differences between quantitative and qualitative approaches, research questions, and thought processes. Schmidt argues that quantitative methods are appropriate for tactical engagements and can inform strategic thinking, but a completely different thought process (i.e., qualitative) is necessary for success when asking questions with strategic implications. Schmidt also discusses how time delays can affect the perceived impact of qualitative modes of thinking."

Predicting Dyspnea Inducers By Molecular Topology, María Gálvez-Llompart,, Jorge Gálvez, Ramón García-Domenech, Lemont B. Kier

Study of Biological Complexity Publications

QSAR based on molecular topology (MT) is an excellent methodology used in predicting physicochemical and biological properties of compounds. This approach is applied here for the development of a mathematical model capable to recognize drugs showing dyspnea as a side effect. Using linear discriminant analysis, it was found a four-variable regression equations enabling a predictive rate of about 81% and 73% in the training and test sets of compounds, respectively. These results demonstrate that QSAR-MT is an efficient tool to predict the appearance of dyspnea associated with drug consumption.

The Influence Of Hypoxia And Ph On Bioluminescence Imaging Of Luciferase-Transfected Tumor Cells And Xenografts, Ashraf A. Khalil, Mark J. Jameson, William C. Broaddus, Peck Sun Lin, Seth M. Dever, Sarah E. Golding, Elisabeth Rosenberg, Kristoffer Valerie, Theodore D. Chung

Neurosurgery Publications

Bioluminescence imaging (BLI) is a relatively new noninvasive technology used for quantitative assessment of tumor growth and therapeutic effect in living animal models. BLI involves the generation of light by luciferase-expressing cells following administration of the substrate luciferin in the presence of oxygen and ATP. In the present study, the effects of hypoxia, hypoperfusion, and pH on BLI signal (BLS) intensity were evaluated in vitro using cultured cells and in vivo using a xenograft model in nude mice. The intensity of the BLS was significantly reduced in the presence of acute and chronic hypoxia. Changes in cell density, viability, and …

Vol. 25 No. 2 Semester 2 (2013)

In Principio - 2010s

No abstract provided.

Virginia Commonwealth University Fall Commencement Program [2013], Virginia Commonwealth University

VCU Commencement Programs

No abstract provided.

Synthesis And Characterization Of Hydroxyapatite Doped With Strontium Carbonate Thin Film Coatings, Stephen Ayodeji Ajinola

Theses

The main purpose of the research is to investigate the corrosion rate and mechanical properties of thin film of strontium carbonate (Sr2CO3) doped with hydroxyapatite (HA) on titanium substrates using DC Corrosion of Potentiodynamic and Nanoindentation respectively. The variation in the weight percentages of strontium carbonate in hydroxyapatite on a titanium substrate was used to investigate the beneficial effect of strontium on surface modification in biological application such as to improve hardness of dentures and prevention of cavities, menopause bone loss and enhancement of remodeling. The corrosion rate, the hardness and elastic moduli of different weight percents of variation in …

Research At The University Of Nebraska–Lincoln: 2012 - 2013 Report, Vicki Miller , Editor

Office of Research and Economic Development: Publications

Contents

Innovative Collaborations Drive Success ... 1

Unraveling Secrets of Brain, Biology and Behavior ... 2

Research, Athletics Partnership ... 4

Understanding Impact of Toddler Sleep Habits ... 5

For Nonparticipants, Voting May Just Be Too Stressful ... 6

Collaborating on Nanoelectronics ... 8

Aftercare Critical for At-risk Teens ... 9

Defense-related Research Expanding ... 10

Reporting Key to Preventing Violence ... 11

Book Opens Cather Letters ... 12

Pioneering New Era for Literary Scholarship ... 14

Lizard Fossil Reveals Climate Clues ... 15

Innovation Campus Emerging ... 16

Research Leading to Water-wise Sensor ... 17

Bolstering Plants’ Defenses ... …

Major Sponsored Programs And Faculty Awards For Research And Creative Activity: July 1, 2012 – June 30, 2013, Vicki Miller , Editor

Office of Research and Economic Development: Publications

This twelfth annual “Major Sponsored Programs and Faculty Awards for Research and Creative Activity” booklet highlights the successes of the University of Nebraska–Lincoln faculty during the fiscal year July 1, 2012-June 30, 2013. It lists the funding sources, projects and investigators on major grants and sponsored program awards received during the year; published books and scholarship; fellowships and other recognitions; intellectual property licenses; and performances and exhibitions in the fine and performing arts.

This impressive list grows each year and I am pleased to present evidence of our faculty’s accomplishments. Grants and contracts in a diverse range of fields—from education …

Spectroscopic Study Of The Use Of Lanthanide Metalloporphyrins As Sensors For Benzene And Acetonitrile Detection In Aqueous Studies, Jr Carlos Crawford

Theses

This work entails the research on lanthanide metalloporphyrins for their potential use as chemical sensors for benzene and acetonitrile. This research is of importance due to the health implications that benzene and acetonitrile cause; benzene is a known carcinogen and acetonitrile is a known lung irritant. The use of UV-Vis spectroscopy, Fluorescence spectroscopy, Gaussian DFT, and X-ray diffraction crystallography were used in the characterization and analysis of the lanthanide porphyrin complexes. Europium, terbium, dysprosium, cerium, and gadolinium were the lanthanides used in conjunction with 5,10,15,20-tetraphenylporphyrin, TPP and 5,10,15,20 tetrakissulfonato porphyrin, TBSP. Based on the luminescence spectroscopy and UV-Vis spectroscopy data, …

From Single-Snp To Wide-Locus: Genome-Wide Association Studies Identifying Functionally Related Genes And Intragenic Regions In Small Sample Studies, Knut M. Wittkowski, Vikas Sonakya, Tingting Song, Martin P. Seybold, Mehdi Keddache, Martina Durner

Krueger Laboratory

Background: Genome Wide Association Studies (GWAS) have had limited success when applied to complex diseases. Analyzing SNPs individually requires several large studies to integrate the often divergent results. In the presence of epistasis, multivariate approaches based on the linear model (including stepwise logistic regression) often have low sensitivity and generate an abundance of artifacts. Methods: Recent advances in distributed and parallel processing spurred methodological advances in non-parametric statistics. U-statistics for multivariate data (μStat) are not confounded by unrealistic assumptions (linearity, independence). Results: By incorporating knowledge about relationships between SNPs, μGWAS (GWAS based on μStat) can identify clusters of genes around …

Physical Therapy 2013, Nova Southeastern University

Health Professions Divisions Course Catalogs and Course Descriptions

No abstract provided.

Eastern Illinois University Undergraduate Catalog 2013 - 2014, Eastern Illinois University

Eastern Illinois Catalog Bulletins

This Catalog lists available courses for the 2013-2014 term.

Homo-Dimerization And Ligand Binding By The Leucine-Rich Repeat Domain At Rhg1/Rfs2 Underlying Resistance To Two Soybean Pathogens, Ahmad Walid Afzali, Ali Srour, Abhishek Goil, Sheeja Vasudaven, Tianyun Liu, Ram Samudrala, Navneet Dogra, Punit Kohli, Ayan Malakar, David A. Lightfoot

Articles

Background: The protein encoded by GmRLK18-1 (Glyma_18_02680 on chromosome 18) was a receptor like kinase (RLK) encoded within the soybean (Glycine max L. Merr.) Rhg1/Rfs2 locus. The locus underlies resistance to the soybean cyst nematode (SCN) Heterodera glycines (I.) and causal agent of sudden death syndrome (SDS) Fusarium virguliforme (Aoki). Previously the leucine rich repeat (LRR) domain was expressed in Escherichia coli.

Results: The aims here were to evaluate the LRRs ability to; homo-dimerize; bind larger proteins; and bind to small peptides. Western analysis suggested homo-dimers could form after protein extraction from roots. The purified LRR domain, from …

Snp-Based Genetic Linkage Map Of Soybean Using The Soysnp6k Illumina Infinium Beadchip Genotyping Array, Masum Akond, Shiming Liu, Lauren Schoener, James A. Anderson, Stella K. Kantartzi, Khalid Meksem, Qijian Song, Dechun Wang, Zixiang Wen, David A. Lightfoot, My Abdelmajid Kassem

Articles

This study reports a high density genetic linkage map based on the ‘Maryland 96-5722’ by ‘Spencer’ recombinant inbred line (RIL) population of soybean [Glycine max (L.) Merr.] and constructed exclusively with single nucleotide polymorphism (SNP) markers. The Illumina Infinium SoySNP6K BeadChip genotyping array produced 5,376 SNPs in the mapping population, with a 96.75% success rate. Significant level of goodness-of-fit for each locus was tested based on the observed vs. expected ratio (1:1). Out of 5,376 markers, 1,465 SNPs fit the 1:1 segregation rate having ≤20% missing data plus heterozygosity among the RILs. Among this 1,456 just 657 were polymorphic …

Identifying Pathogenic Salmonella Serotypes Isolated From South Central Va Waterways Via Sequential Pcr Analysis, Timothy M. Smith, Jr.

Theses & Honors Papers

Researchers often debate whether all serovars of Salmonella enterica are pathogenic to humans. Broiler houses containing chickens are a major source of Salmonella and serve as potential reservoirs. Therefore, farmers must take substantial measures in order to avoid contamination with their products. The primary objective of the study was to determine whether the four most clinically relevant Salmonella spp. serovars from poultry houses could be isolated from the environmental waterways in Prince Edward and Cumberland Counties of Virginia. The study adapted a serotyping method designed for typing Salmonella to gain a perspective of possible serovars that may be found in …

In The System: Facilitating The Reunification Of The Child And The Parents Through Religion, Cherie Nicole Brown

Hofstra Law Review

The article discusses the reported efforts to facilitate the reunification of children and parents through religion as of 2014, focusing on terminations of parental rights in America and U.S. state laws regarding Sharia law and international law. The legal rights of Muslims in America are mentioned, along with the U.S. Constitution's Establishment Clause and an alleged Anti-Sharia Movement in the U.S. America's foster care system and the best interest of the child legal standard are examined.

On The Sleeve Of The Visual: Race As Face Value, Alessandra Raengo

Dartmouth College Press

In this work of critical theory, Black studies, and visual culture studies, the author reads race as a theory of the image. By placing emphasis on the surface of the visual as the repository of its meaning, race presents the most enduring ontological approach to what images are, how they feel, and what they mean. Having established her theoretical concerns, the author's eclectic readings of various artifacts of visual culture, fine arts, cinema, and rhetorical tropes provoke and destabilize readers' visual comfort zone, forcing them to recognize the unstated racial aspects of viewing and the foundational role of race in …

Adoption Awareness In The Elementary Classroom, Claire Laberge

Capstone Projects and Master's Theses

Adoption is a complex and delicate issue, and too often, outdated and biased attitudes toward adoption influence people’s perceptions of adoption and adoptees. Adopted children are particularly vulnerable to negativity toward the adoptive family structure, and adoptees have special emotional needs that must be addressed in the elementary school classroom. Research demonstrates a variety of ways teachers can bring awareness to adoption issues, de-stigmatize adoption in the classroom, and in turn, create a safe and nurturing classroom environment. The researcher designed an anonymous survey to distribute to a sampling of elementary school teachers; the survey results generally supported the researcher’s …

The Perfect Priest: An Examination Of Leviticus 21:17-23, Jared Wilson

Seminary Masters Theses

Leviticus 21:17-23 is a text that discriminates against priests who have one of the twelve blemishes listed, approaching the text with the intent to redeem the text for modern application. An exploration in the role of priests in the ancient Near East will show how the priests were human representations of the deity and many cultures and religions had physical restrictions for the priests similar to Lev 21:17-23. Examining the language of Lev 21:17-23 will show that the restrictions were not merely symbolic but intended to stop blemished priests from approaching the altar. However, they were not statements about the …

Co-Sustenance: An Alternative To Stewardship, Matt Addis

Seminary Masters Theses

This thesis is concerned with the current ecological realities and the inability of current stewardship construct's to adequately address these problems. This study addresses the underlying weaknesses of current articulations of stewardship theologies. It also engages with the scientific theories that form the basis for stewardship theologies, as well as those theories that might offer alternative narratives for ecological and biotic understandings. This study also addresses the underlying ethical realities of current ecological crises. Finally this thesis seeks to offer an alternative to traditional Christian understandings of the relationship of humanity to nature. Chapter one introduces the reader to the …

Towards Understanding Cytotoxicity And Cellular Uptake Of Engineered Nanomaterials, Komal Sudam Garde

Theses

With a widespread use of nanomaterials in various manufacturing applications including consumer goods, semiconductor industry and pharmaceuticals, the safety of Engineering Nanomaterials (ENs) is a vital area of research. It is extremely important to analyze the risks posed by ENs in order to optimize design and/or control their use. The overarching goal of this work is to understand the cytotoxicity and cellular uptake potential of specific EN of well characterized physicochemical properties. Towards this objective, the specific aims of this project are to (a) comprehensively characterize the physical and chemical properties of starting ENs, (b) study their dose and time-course …

Product Comparison Of Foliar Fungicide Efficacy On Diseases Of Field Corn In Nebraska, 2013, C. M. Schleicher, T. A. Jackson-Ziems

Department of Plant Pathology: Faculty Publications

A foliar fungicide efficacy trial was conducted at the University of Nebraska-Lincoln South Central Agricultural Laboratory near Clay Center, NE. Corn hybrid NK ‘N68B’, rating of “fair” (7 out of 9) for gray leaf spot (GLS), not rated for common rust (CR), and “excellent” (2 out of 9) for southern rust (SR), was planted on 15 May in 30-in. rows at a target population of 31,763 plants/A. The trial area was disked with corn as the previous year’s crop. Eight treatments and a non-treated control were replicated six times in a randomized complete block design. Each plot was four rows …

University Update - October 23, 2013, Academic Affairs

University Updates & Communications from the University President

Included in this update are:

• Board of Trustees Meeting
• Provost/Vice President Search Underway
• Parking Lot Survey
• Join the Conversation

Parallel Changes In Genital Morphology Delineate Cryptic Diversification Of Planktonic Nudibranchs, Alvin Alejandrino, Celia K. C. Churchill, Ángel Valdés, Diarmaid Ó. Foighil

Biology

The relative roles of geographical and non-geographical barriers in the genesis of genetic isolation are highly debated in evolutionary biology, yet knowing how speciation occurs is essential to our understanding of biodiversity. In the open ocean, differentiating between the two is particularly difficult, because of the high levels of gene flow found in pelagic communities. Here, we use molecular phylogenetics to test the hypothesis that geography is the primary isolating mechanism in a clade of pelagic nudibranchs, Glaucinae. Our results contradict allopatric expectations: the cosmopolitan Glaucus atlanticus is panmictic, whereas the Indo-Pacific Glaucus marginatus contains two pairs of cryptic species …

Cell-Autonomous Sex Determination Outside Of The Gonad, Jenny C. Link, Arthur P. Arnold, Xuqi Chen, Yuichiro Itoh, Karen Reue

Biology

The classic model of sex determination in mammals states that the sex of the individual is determined by the type of gonad that develops, which in turn determines the gonadal hormonal milieu that creates sex differences outside of the gonads. However, XX and XY cells are intrinsically different because of the cell-autonomous sex-biasing action of X and Y genes. Results: Recent studies of mice, in which sex chromosome complement is independent of gonadal sex, reveal that sex chromosome complement has strong effects contributing to sex differences in phenotypes such as metabolism. Adult mice with two X chromosomes (relative to mice …

Relational Discernment : Moving Towards A Holistic Approach, Vonna Thomas

Doctor of Ministry

No abstract provided.

Highly Efficient Quenching Of Nanoparticles For The Detection Of Electron-Deficient Nitroaromatics, Patrick Marks, Sage Cohen, Mindy Levine

Chemistry Faculty Publications

Reported herein is the highly efficient quenching of fluorescent organic nanoparticles by 2,4-dinitrotoluene (DNT) and 2,4,6-trinitrotoluene (TNT). These fluorescent nanoparticles are formed from the hydrophobic collapse of fluorescent polymer chains, and display quenching efficiencies that are in line with the highest reported literature values. Moreover, the fluorescent quenching occurs only for the fluorescent nanoparticles, and not for the precursor polymer solutions, which display marked insensitivity to the presence of nitroaromatics. This aggregation-dependent fluorescent quenching has numerous applications for the detection of small-molecule electron-deficient analytes.

Que Ondee Sola - January 2013, Alyssa N. Villegas

Que Ondee Sola

Student publication founded in 1972, articles include: Historical Reflection of a Practioner, Critical Pedagogy Community Building and Self-Determination, Siempre Pa'lante: Celebrating 40 Years of Commitment, Greening the Rooftops of Paseo Boricua, A Movement to Empower Young Families, Who was Ferd Eggan?, Dreaming in Ouerto Rican, La Escuelita: Home in Community, Alumni Well Wishes, Somos Fruto de Nuestro Ayer Program, A Few Hours a Week, Student Write to Oscar Lopez, Transformation Through Engagement, Yo Recuerda a Irma, Creating a Decolonizing and Culturally Relevant Curriculum, Living Breathing Solidarity, Sembrando Semillas, Lucha y Resistencia: the Foundations of a Legacy

Intellectual Property And Public Health – A White Paper, Ryan G. Vacca, James Ming Chen, Jay Dratler Jr., Thomas Folsom, Timothy S. Hall, Yaniv Heled, Frank A. Pasquale, Elizabeth A. Reilly, Jeffery Samuels, Katherine J. Strandburg, Kara W. Swanson, Andrew W. Torrance, Katharine A. Van Tassel

Faculty Scholarship

On October 26, 2012, the University of Akron School of Law’s Center for Intellectual Property and Technology hosted its Sixth Annual IP Scholars Forum. In attendance were thirteen legal scholars with expertise and an interest in IP and public health who met to discuss problems and potential solutions at the intersection of these fields. This report summarizes this discussion by describing the problems raised, areas of agreement and disagreement between the participants, suggestions and solutions made by participants and the subsequent evaluations of these suggestions and solutions. Led by the moderator, participants at the Forum focused generally on three broad …