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Sarcoptes Scabiei Mites Modulate Gene Expression In Human Skin Equivalents, Marjorie S. Morgan, Larry G. Arlian, Michael P. Markey

Biochemistry and Molecular Biology Faculty Publications

The ectoparasitic mite, Sarcoptes scabiei that burrows in the epidermis of mammalian skin has a long co-evolution with its hosts. Phenotypic studies show that the mites have the ability to modulate cytokine secretion and expression of cell adhesion molecules in cells of the skin and other cells of the innate and adaptive immune systems that may assist the mites to survive in the skin. The purpose of this study was to identify genes in keratinocytes and fibroblasts in human skin equivalents (HSEs) that changed expression in response to the burrowing of live scabies mites. Overall, of the more than 25,800 …

Serials Solutions Subject Search Report 2012-2013, Andrée J. Rathemacher

Technical Services Reports and Statistics

Statistics on the total number of Serials Solutions subject searches at the University of Rhode Island Libraries by month for 2012-2013. Shows how many times the A-Z full text electronic journal list (at http://zu4tq4pb5v.search.serialssolutions.com/) was browsed by specific subjects.

2013 Annual Meeting Program

Journal of the South Carolina Academy of Science

No abstract provided.

Evaluation Of The Efficacy Of 1-Octen-3-Ol And Carbon Dioxide Chemoattractants With Mosquitoes And Bloodmeal Analysis Of Culex Mosquito Spp. In Lancaster County, Nebraska, Julianne N. Matczyszyn

Department of Entomology: Dissertations, Theses, and Student Research

The purpose of mosquito-borne disease surveillance is to assess the risk of pathogen transmission by assessing mosquito populations and the prevalence of disease pathogens in those populations. West Nile virus (WNV) is an important mosquito-borne virus in Nebraska, and can be transmitted by several mosquito species found in Lancaster County, Nebraska, including Culex pipiens L., Culex salinarius Coquillett, Culex restuans Theobald, Culex tarsalis Coquillett, Culex territans Walker, and Culex erraticus Dyar & Knab. These species are ornithophilic, yet many studies indicate a shift in host feeding to mammalian in late summer months. One-octen-3-ol (octenol) can be isolated …

Development Of Local-Based Mdgs & Arg Models On Poor Woman, Wanto Rivaie, Nuraini Asriati

BISNIS & BIROKRASI: Jurnal Ilmu Administrasi dan Organisasi

This research studies the local-based MDGs and ARG models of poverty eradication efforts in border areas in West Borneo. The aim is to map the psycho-social economic dimensions and identify the behavior of poverty-stricken groups. Poverty in border areas is getting higher compared to other areas since they are strategically and geographically different in characteristics. The purpose of the study is to improve the policy and budgeting system of poverty eradication programs. A qualitative approach is used through the concept of planning and developing. The result shows that the high income of some people does not reduce the number of …

The Design And Fabrication Of An Electrostatically Actuated Diaphragm With A Silicon-On-Insulator Wafer, Elizabeth L. Brooks

Master's Theses

Electrostatically actuated silicon membranes were designed, modeled, fabricated, and characterized. The intended application was for use in a microspeaker. Fabrication issues necessitated the use of thick diaphragms with a large gap between the electrodes. The devices did not function as speakers but did show actuation with a high DC voltage. Device dimensions were chosen by examining membrane mechanics, testing the processing steps required for device fabrication, and modeling with COMSOL. Several adhesives were researched to fabricate the device sidewalls, including BCB, PMMA, and TRA-Bond F112. A method for patterning PMMA through photolithography was found using a scanning electron microscope. Masks …

Transgenic Sickle Cell Disease Mice Have High Mortality And Dysregulated Immune Responses After Vaccination, Steven M. Szczepanek, Eric R. Secor Jr, Sonali J. Bracken, Linda Guernsey, Ektor Rafti, Adam Matson, Roger S. Thrall

UCHC Articles - Research

Background

Children with sickle cell disease (SCD) are susceptible to recurrent infections, which are often life threatening and necessitate frequent vaccinations. Given the altered baseline immunity and proinflammatory state associated with SCD, we sought to determine the relative safety and efficacy of vaccination in transgenic SCD mice.

Methods

Eight week-old SCD mice were vaccinated with ovalbumin (OVA) and aluminum hydroxide weekly for three weeks by the intraperitoneal (IP) or intramuscular (IM) route. One week after the third vaccination, serum cytokines/chemokines, immunoglobulins, and bronchoalveolar lavage (BAL) fluid cytokines were measured.

Results

Only SCD mice were prone to mortality associated with vaccination …

Progress Notes, Lehigh Valley Health Network

Progress Notes

No abstract provided.

Undergraduate And Graduate Course Descriptions, 2013 Fall, Wright State University

Course Descriptions

Wright State University undergraduate and graduate course descriptions from Fall 2013.

A Teacher's Guide To Pisa Scientific Literacy, Sue Thomson, Kylie Hillman, Lisa De Bortoli

OECD Programme for International Student Assessment (PISA) Australia

This report focuses on Australian students' performance on the PISA items that have been released in Scientific Literacy. It includes an overview of the PISA Scientific Literacy Framework, Australian students' results in the 2006 international assessment, examples of responses and marking guides and the context behind achievement, e.g. attitudes, engagement and motivation, and recognition of environmental issues.

Structural, Antigenic, And Evolutionary Characterizations Of The Envelope Protein Of Newly Emerging Duck Tembusu Virus, Kexoang Yu, Zhi-Zhang Sheng, Bing Huang, Xiuli Ma, Yufeng Li, Xiaoyuam Yuan, Zhuoming Qin, Dan Wang, Suvobrata Chakravarty, Feng Li, Minxun Song, Huaichang Sun

Chemistry and Biochemistry Faculty Publications

Since the first reported cases of ducks infected with a previously unknown flavivirus in eastern China in April 2010, the virus, provisionally designated Duck Tembusu Virus (DTMUV), has spread widely in domestic ducks in China and caused significant economic losses to poultry industry. In this study, we examined in detail structural, antigenic, and evolutionary properties of envelope (E) proteins of six DTMUV isolates spanning 2010–2012, each being isolated from individual farms with different geographical locations where disease outbreaks were documented. Structural analysis showed that E proteins of DTMUV and its closely related flavivirus (Japanese Encephalitis Virus) shared a conserved array …

Flight Physician - August, 2013, Civil Aviation Medical Association

Browse all Civil Aviation Medical Association Newsletters

A seventeen page newsletter of the Civil Aviation Medical Association. The newsletter provided news about civil aviation medicine and information related to the organization.

Prevalence Of High-Risk Human Papilloma Virus Among Women With Hepatitis C Virus Before Liver Transplantation, P. A. Tarallo, Janice Smolowitz, D. Carriero, J. Tarallo, A. Siegel, H. Jia, J. C. Emond

School of Nursing Faculty Scholarship and Creative Works

Background: We sought to assess the prevalence and risk factors for high-risk human papillomavirus (HPV) infection among female liver transplant (LT) candidates. Traditional health screening before LT listing has included Pap smear and is typically carried out by the patient's local provider. The prevalence of high-risk HPV in this population has not been studied. Methods: With Institutional Review Board approval, 62 LT candidates received a liquid-based Pap smear with high-risk HPV testing as part of their pre-transplant evaluation by a single provider. Clinical variables included age, ethnicity, insurance status, prior Pap smear, and HPV results, HPV risk factors including age …

Hearing Loss In Perinatally Hiv-Infected And Hiv-Exposed But Uninfected Children And Adolescents, Peter Torre Iii, J. Hoffman, Ashley L. Buchanan, George K. Silberry, Mabel Rice, Patricia A. Sirois, Paige L. Williams

Pharmacy Practice and Clinical Research Faculty Publications

Background - Little is known about hearing loss in children with HIV infection (HIV+). We examined the prevalence of hearing loss in perinatally HIV+ and HIV-exposed but uninfected (HEU) children, compared these to the percentage with hearing loss in the general population, and evaluated possible risk factors for hearing loss in HIV+ and HEU children.

Methods - Audiometric examinations were completed in children who met any pre-specified criteria for possible hearing loss. The hearing examination consisted of a tympanogram in each ear and pure-tone air-conduction threshold testing from 500 through 4000 Hz. Hearing loss was defined as the pure-tone average …

Individual Behavioral Variation Of Juvenile Spiny Lobster (Panulirus Argus) Denning Behaviors And The Role It Plays In Shelter Competition During Habitat Loss, Katherine Heldt

All Theses

Variation in juvenile spiny lobster (Panulirus argus) aggressive and gregarious behaviors may play an important role in structuring population level interactions. Since aggressive and gregarious behaviors were not repeatable and were found to be highly correlated with size, these behaviors were found to be largely driven by a combination of behavioral plasticity and ontogeny. Although larger individuals were found to be the most aggressive and least gregarious individuals, often occupying crevice shelters by themselves, they did not exclude smaller, less aggressive lobsters from crevice shelters. Surprisingly, in shelter limited situations, small, less aggressive individuals were more likely to use dens …

Cause And Impacts Of The Early Season Collapse Of Lilium Grayi (Gray’S Lily), On Roan Mountain, Tn/Nc, Russell J. Ingram

Electronic Theses and Dissertations

A population of the rare Southern Appalachian endemic species Lilium grayi, (Gray’s lily) Roan Mountain, TN/NC was monitored for 2 years to determine the cause and impact of an early season collapse. High concentrations of the Lilium spp. host-specific fungal phytopathogen, Pseudocercosporella inconspicua (G. Winter) U. Braun were associated with 19/20 symptomatic and 0/30 asymptomatic plants. Strength of the association between pathogen and disease and the replication of disease symptoms in 4/4 healthy hosts showed that P. inconspicua was the causal agent of the disease referred to as lily leaf spot. Disease had a severe impact on the population …

The Best Practices For Teaching Writing To Postsecondary Students With Acquired Brain Injuries, Julianne Candio Sekel

Theses, Dissertations and Culminating Projects

Because the writing abilities of postsecondary students with acquired brain injuries (ABI) are often determined by the student’s age when the injury was acquired, the severity of the injury, the amount of time that has passed since the injury, and the quality of the student’s writing education before the injury, it is impossible to generalize the best strategies to assist students with ABI in writing. However, through a review of existing literature on teaching writing to students with ABI, the relationship between oral and written discourse, expressive writing, educational intervention, and assistive technologies, this study presents a list of recommendations …

Expert–Novice Differences In Mental Models Of Viruses, Vaccines, And The Causes Of Infectious Disease, Benjamin D. Jee, David H. Uttal, Amy N. Spiegel, Judy Diamond

World of Viruses

Humans are exposed to viruses everywhere they live, play, and work. Yet people’s beliefs about viruses may be confused or inaccurate, potentially impairing their understanding of scientific information. This study used semi-structured interviews to examine people’s beliefs about viruses, vaccines, and the causes of infectious disease. We compared people at different levels of science expertise: middle school students, teachers, and professional virologists. The virologists described more entities involved in microbiological processes, how these entities behaved, and why. Quantitative and qualitative analyses revealed distinctions in the cognitive organization of several concepts, including infection and vaccination. For example, some students and teachers …

Conflicts Of Interest And The Quality Of Recommendations In Clinical Guidelines, Lisa Cosgrove, Allen F. Shaughnessy, Deborah R. Erlich, Emily E. Wheeler, Harold J. Bursztajn

Counseling, School Psychology & Sport Faculty Publication Series

Background: There is increasing concern that conflicts of interest affect the development process of clinical practice guidelines. We evaluated The American Psychiatric Association's Practice Guideline for the Treatment of Patients with Major Depressive Disorder to determine the existence of financial and intellectual conflicts of interest and examine their possible effects. We selected this guideline because of its influence on clinical practice and because this guideline recommends pharmacotherapy for all levels of depression, despite controversies over the evidence base.

Methods and Findings: We determined the number and type of financial conflicts of interest for members of the guideline development group as …

Controlling Bacterial Persister Cells And Biofilms By Synthetic Brominated Furanones, Jiachuan Pan

Biomedical and Chemical Engineering - Dissertations

Bacteria are well known to obtain tolerance to antibiotics by forming multicellular structures, known as biofilms, and by entering dormancy and forming persister cells. Both mechanisms allow bacteria to tolerate antibiotics at concentrations hundreds to thousands of times higher than the lethal dose for regular planktonic cells of the same genotype. Persister formation increases in biofilms; thus, effective control of persister cells, especially those in biofilms is critically important to infection control. Over the past decades, a bacterial signaling system based on cell density, named quorum sensing (QS), has been found to regulate biofilm formation and, in Pseudomonas aeruginosa, the …

Disease Ecology Of A Microsporidian Parasite And Its Effects On Mottled Sculpin, Jared Joseph Homola

Masters Theses

Infectious disease can influence organisms at all levels of ecological organization, from individuals to ecosystems. Likewise, the ecosystems where pathogens exist directly influence their success. Recent theoretical studies have tied disease prevalence to biotic factors such as genetic diversity, biodiversity, and host behavior, and abiotic factors that include temperature and increased nutrient concentrations. Parasites included in the phylum Microspora are increasingly recognized for being ubiquitous in nature, although their ecological roles are generally unknown. This study examined several environmental, community, and host-related metrics to compare the biotic and abiotic aspects of 16 small streams; 6 with mottled sculpin (Cottus …

Ultrasound Based Evaluation Of Hepatic Steatosis And Fibrosis In Hepatitis C Non-Responders, Saba Sohail, Sina Aziz, Talat Mirza

Department of Radiology

Objective: To determine the accuracy of ultrasound in the diagnosis and grading of steatosis and fibrosis in Hepatitis C (HCV) patients not responding to ribavarin-interferon therapy.
Study design: A cross-sectional, analytical study.
Place and duration of study: Radiology Department, Civil Hospital, Karachi, from March 2008 to August 2010.
Methodology: Patients with positive HCV RNA despite 24 weeks ribavarin-interferon therapy (non-responders) were subjected to ultrasound and biopsy prior to institution of pegylated interferon therapy for detection and grading of steatosis and fibrosis. Using histopathology as the gold standard, sensitivity, specificity, negative and positive predictive values for ultrasound were determined.
Results: The …

An Economic Evaluation Of Primary Care Behavioral Health In Pediatrics: A Case Study, Natasha B. Gouge

Electronic Theses and Dissertations

A barrier cited by primary care administrators in integrating behavioral health is financial risk. Fee-for-service billing mechanisms remain complex and there is little empirical guidance on cost-effective models. This study was an economic evaluation of an integrated care model in a pediatric private practice clinic. The study evaluated cost benefits by examining specific delivery indices such as concerns presented, time spent, billing codes used, and reimbursement received in regards to pediatric primary care visits by comparing days when an on-site Behavioral Health Consultant (BHC) was available versus Non-BHC Days. All 3 hypotheses were supported: 1) more patients were seen in …

The Rebalance To Asia: U.S.-China Relations And Regional Security, Phillip C. Saunders

Strategic Forums

Upon taking office in January 2009, Obama administration officials proclaimed a U.S. “return to Asia.” This pronouncement was backed with more frequent travel to the region by senior officials (Secretary of State Hillary Clinton’s first trip was to Asia) and increased U.S. participation in regional multilateral meetings, culminating in the decision to sign the Association of Southeast Asian Nations (ASEAN) Treaty of Amity and Cooperation and to participate in the East Asia Summit (EAS) at the head-of-state level. The strategic “rebalance to Asia” announced in November 2011 builds on these earlier actions to deepen and institutionalize U.S. commitment to the …

Role Of Msa In Immune Evasion, Persistence, And Protease Regulation In The Human Pathogenic Strains Of Staphylococcus Aureus, Maria Deepa Basco

Dissertations

Opportunistic pathogens like Staphylococcus aureus on entering the host can stay colonized at the foci of infection or evade the immune system to disseminate to other sites. In this study we investigated the regulatory influence of the modulator of sarA (msa) on immune evasion and host persistence, employing the hospital-acquired strain S. aureus UAMS-1 and community-acquired strain S. aureus USA300 LAC. In the murine sepsis model, mutation of the msa gene in LAC showed no change in dissemination of infection; however, in UAMS-1 a decrease in microbial load was observed in the lungs. Differential regulation by the msa gene was …

Characteristics Of White-Tailed Deer Visits To Cattle Farms: Implications For Disease Transmission At The Wildlife-Livestock Interface, Are R. Berentsen, Ryan S. Miller, Regina Misiewicz, Jennifer L. Malmberg, Mike R. Dunbar

Zoonotics and Wildlife Disease: Publications

Bovine tuberculosis (bTB) is endemic in free-ranging white-tailed deer (Odocoileus virginianus) in MI, USA. Currently, the rates of farm visitation by deer and co-use of forage resources by cattle and deer are poorly understood. To evaluate the extent deer and livestock may share forage resources, we investigated farm, yard, and cattle-use area visitation by white-tailed deer and compared visitation with common livestock management practices. We fitted 25 female white-tailed deer near the bTB-infected zone in Michigan’s Lower Peninsula with global positioning system collars. Livestock management practices associated with farm visitation included presence of confined feeding pastures, number of …

Rrh Library Newsletter, August 2013, Libraries At Rochester Regional Health

Rochester Regional Health authored publications and proceedings

Newsletter sections include: Foodborne Diseases; Announcing UpToDate Anywhere; Connect to Library Resources from EPIC

Analysis Of Post-Cranial Traits For The Assessment Of Biological Distance Among Temporally Distinct New World Populations Of African Descent, Claire Marie Dansereau

Master's Theses

This investigation examines change in post-cranial osteometric markers in temporally distinct New World populations of African ancestry: Newton Plantation series (17th-19th century Barbados; N = 16); two subsamples from the Hamann-Todd (HT) series (U.S.) based on birth year, 1865-1900 (N = 66) and 1901-1915 (N = 62); and the Forensic Databank Collection (FDB) (20th century U.S.; N = 137). It was anticipated that individuals from Newton would exhibit less admixture than those from H-T or FDB. They were also predicted to demonstrate greater skeletal robusticity due to mechanical loading. Additionally, it was expected that stature would increase over time due …

Microsporidia Spore Adherence And Host Cell Infection In Vitro, Cory A. Leonard

Electronic Theses and Dissertations

Microsporidia infect invertebrate and vertebrate animals. Human pathogenic microsporidia are associated with severe disease in immunocompromised individuals, and mostly asymptomatic infection in the immunocompetent. Treatment options for microsporidiosis are limited, incompletely effective, and associated with toxicity. Furthermore, microsporidia infection of healthy individuals is poorly understood, and the consequences of asymptomatic infection have not been determined. Little is known about the molecular mechanisms of microsporidia infection, but such information is essential for the development of new therapies. Spores adhere to host cell surfaces in vitro. Our laboratory has focused on determining specific host cell and microsporidia spore surface participants in spore …

Bioengineering The Expression Of Active Recombinant Human Cathepsin G, Enteropeptidase, Neutrophil Elastase, And C-Reactive Protein In Yeast, Eliot T. Smith

Electronic Theses and Dissertations

The yeasts Pichia pastoris and Kluyveromyces lactis were used to express several recombinant human proteins for further biochemical characterization. Two substitution variants of recombinant human enteropeptidase light chain (rhEPL) were engineered to modify the extended substrate specificity of this serine protease. Both were secreted as active enzymes in excess of 1.7 mg/L in P. pastoris fermentation broth. The substitution variant rhEPL R96Q showed significantly reduced specificities for the preferred substrate sequences DDDDK and DDDDR; however, the rhEPL Y174R variant displayed improved specificities for these substrate sequences relative to all other reported variants of this enzyme. The neutrophil serine proteases human …