Digital Commons Network^™

Association Between Antibodies To Multiple Infectious And Food Antigens And New Onset Schizophrenia Among Us Military Personnel, Yuanzhang Li, Natalya S. Weber, Jared A. Fisher, Robert H. Yolken, David N. Cowan, Rakel A. Larsen, David W. Niebuhr

Uniformed Services University of the Health Sciences

Introduction: Multiple studies have documented immune activation in many individuals with schizophrenia suggesting that antigens capable of generating a prolonged immune response may be important environmental factors inmany cases of this disorder.While existing studies have found single-agent associations of antibodies to food and neurotropic infectious agents with schizophrenia, a simultaneous examination of multiple agents may shed light on agent interactions or possible etiopathogenic pathways. Methods: We used traditional regression and novel statistical techniques to examine associations of single and combined infectious and food antigens with schizophrenia. We tested 6106 serum samples from 855 cases and 1165 matched controls. …

Relevance Of Molecular Mimicry In The Mediation Of Infectious Myocarditis, Chandirasegaran Massilamany, Sally A. Huber, Madeleine W. Cunningham, Jay Reddy

Jay Reddy Publications

Heart disease, the leading cause of death in humans, is estimated to affect one in four American adults in some form. One predominant cause of heart failure in young adults is myocarditis, which can lead to the development of dilated cardiomyopathy, a major indication for heart transplantation. Environmental microbes, including viruses, bacteria, and fungi that are otherwise innocuous, have the potential to induce inflammatory heart disease. As the list is growing, it is critical to determine the mechanisms by which microbes can trigger heart autoimmunity and, importantly, to identify their target antigens. This is especially true as microbes showing structural …

Isolation And Genomic Characterization Of Chaoyang Virus Strain Rok144 From Aedes Vexansnipponii From The Republic Of Korea, John S. Lee, Nathan D. Grubaugh, John P. Kondig, Michael J. Turell, Heung-Chul Kim, Terry A. Klein, Monica L. O’Guinn

United States Army: Publications

During June 2003, mosquito surveillance was conducted at a US Army installation and a US Military training site 2 km south of the demilitarized zone, Republic of Korea. Mosquitoes were collected using Mosquito Magnets^TM, sorted to species, and assayed for the presence of arboviruses. From the 3,149 mosquitoes that were sorted into126 pools, one Aedes vexan snipponii pool (out of 73 pools) tested positive for flavivirus RNA by reverse transcription-PCR. After isolation from C6/36 cell culture supernatant, the viral genome was sequenced and found to be 98.9% related to Chaoyang virus, a potential arthropod-specific flavivirus. This report details …

Construction And Nonclinical Testing Of A Puumala Virus Synthetic M Gene-Based Dna Vaccine, R. L. Brocato, M. J. Josleyn, V. Wahl-Jensen, C. S. Schmaljohn, J. W. Hooper

United States Army: Publications

Puumala virus (PUUV) is a causative agent of hemorrhagic fever with renal syndrome (HFRS). Although PUUV-associated HFRS does not result in high case-fatality rates, the social and economic impact is considerable. There is no licensed vaccine or specific therapeutic to prevent or treat HFRS. Here we report the synthesis of a codon-optimized, full-lengthMsegment open reading frame and its cloning into a DNA vaccine vector to produce the plasmid pWRG/PUU-M(s2). pWRG/PUU-M(s2) delivered by gene gun produced high-titer neutralizing antibodies in hamsters and nonhuman primates. Vaccination with pWRG/ PUU-M(s2) protected hamsters against infection with PUUV but not against infection by related HFRS-associated …

Genome Sequence Of Moraxella Macacae 0408225, A Novel Bacterial Species Isolated From A Cynomolgus Macaque With Epistaxis, Jason T. Ladner, Chris A. Whitehouse, Galina I. Koroleva, Gustavo F. Palacios

United States Army: Publications

Moraxella macacae is a recently described bacterial species that has been associated with at least two outbreaks of epistaxis in macaques. Here we present the first genome sequence of this novel species, isolated from a symptomatic cynomolgus macaque at the U.S. Army Medical Research Institute of Infectious Diseases.

Clinical Use Of Colistin Induces Cross-Resistance To Host Antimicrobials In Acinetobacter Baumannii, Brooke A. Napier, Eileen M. Burd, Sarah W. Satola, Stephanie M. Cagle, Susan M. Ray, Patrick Mcgann, Jan Pohl, Emil P. Lesho, David S. Weiss

United States Army: Publications

The alarming rise in antibiotic resistance has led to an increase in patient mortality and health care costs. This problem is compounded by the absence of new antibiotics close to regulatory approval. Acinetobacter baumannii is a human pathogen that causes infections primarily in patients in intensive care units (ICUs) and is highly antibiotic resistant. Colistin is one of the last-line antibiotics for treating A. baumannii infections; however, colistin-resistant strains are becoming increasingly common. This cationic antibiotic attacks negatively charged bacterial membranes in a manner similar to that seen with cationic antimicrobials of the innate immune system. We therefore set out …

Spatio-Temporal Dynamics Of Pneumonia In Bighorn Sheep, E. Frances Cassirer, Raina K. Plowright, Kezia R. Manlove, Paul C. Cross, Andrew P. Dobson, Kathleen A. Potter, Peter J. Hudson

United States Geological Survey: Staff Publications

1. Bighorn sheep mortality related to pneumonia is a primary factor limiting population recovery across western North America, but management has been constrained by an incomplete understanding of the disease. We analysed patterns of pneumonia-caused mortality over 14 years in 16 interconnected bighorn sheep populations to gain insights into underlying disease processes.

2. We observed four age-structured classes of annual pneumonia mortality patterns: all-age, lamb-only, secondary all-age and adult-only. Although there was considerable variability within classes, overall they differed in persistence within and impact on populations. Years with pneumonia-induced mortality occurring simultaneously across age classes (i.e. all-age) appeared to be …

Hierarchical Networks, Power Laws, And Neuronal Avalanches, Adam Landsberg, Eric J. Friedman

Physics Faculty Scholarship

We show that in networks with a hierarchical architecture, critical dynamical behaviors can emerge even when the underlying dynamical processes are not critical. This finding provides explicit insight into current studies of the brain's neuronal network showing power-lawavalanches in neural recordings, and provides a theoretical justification of recent numerical findings. Our analysis shows how the hierarchical organization of a network can itself lead to power-law distributions of avalanche sizes and durations, scaling laws between anomalous exponents, and universal functions—even in the absence of self-organized criticality or critical points. This hierarchy-induced phenomenon is independent of, though can potentially operate in conjunction …

A Mathematical Modeling Framework For Phlebotomist Scheduling And Blood Draw Assignments In Laboratory Medicine, Laquanda T. Leaven

Dissertations

Laboratory services in healthcare delivery systems play a vital role in inpatient care. Studies have shown that laboratory data affects approximately 65% of the most critical decisions on admission, discharge, and medication. Laboratory testing accounts for approximately 10% of hospital billing. Reducing laboratory costs would contribute to reducing total healthcare cost, which is one of the major goals for the U.S. healthcare delivery system. This research focuses on improving the performance of the hospital laboratory in a large hospital system. The intention of this study is to identify and then optimize the most critical stage to improve the entire laboratory …

Unique Perspectives And Struggles Of Parents Rearing Children With Phenylketonuria With Unaffected Siblings, Cassandra Nicole Hollinger

Theses and Dissertations

Phenylketonuria (PKU) is an autosomal metabolic condition that is screened for via newborn screening. Individuals who are identified as having PKU require a protein-restricted diet to protect against neurological damage. Many parents who learn their child has PKU may already have children who are not affected, or may have children later who are not affected. This creates a unique situation in which parents are rearing children that require a strict protein-restricted diet with children who do not. Parents who are currently rearing children with and without PKU were surveyed to learn what unique perspectives and struggles they face, especially regarding …

Estimation Of Aerosolized Antibiotic Resistant Bacteria Concentrations From Wastewater Treatment Facilities Using Spatially-Based Dispersion Modeling, Renee Nichole Dickman

Theses and Dissertations

Pathogenic bacteria can develop resistance to antibiotics to which they were previously sensitive, resulting in increased morbidity and mortality worldwide [1]. The increase in drug resistance in bacteria is an emerging public health concern. The activated sludge tanks of wastewater treatment plants are known sources of antibiotic resistant bacteria. These resistant bacteria can become aerosolized and disperse downwind. Using previously sampled aerosol concentrations and meteorological data, a Gaussian dispersion model was developed to estimate the concentration downwind from a selected wastewater treatment facility. The emission rate was calculated to be 7,941 CFU/m²/sec ± 2,149 CFU/m²/sec. This value was used in …

Models And Software Development For Interval-Censored Data, Chun Pan

Theses and Dissertations

Interval-censored time-to-event data occur naturally in studies of diseases where the symptoms are not directly observable, and periodic clinical examinations are required for detection. Due to the lack of well-established procedures, interval-censored data have been conventionally treated as right-censored data, however, this introduces bias at the first place. This dissertation focuses on methodological research and software development for interval-censored data. Specifically, it consists of three projects. The first project is to create an R package for regression analysis and survival curve estimation of interval-censored data based on several published papers by our research team. In the second project, a Bayesian …

The Association Of Physical Fitness With Psychological Health Outcomes In Soldiers During Army Basic Combat Training, Shannon K. Crowley

Theses and Dissertations

Despite the current need for investigation of factors involved with soldiers' resilience to stressors in the Basic Combat Training (BCT) environment, and evidence in civilian populations that physical training is associated with psychological benefits, little is known about the relationship between physical fitness and psychological adjustment during BCT. Study one of this dissertation involved an extensive literature review of factors related to the relationship between physical fitness and psychological adjustment of soldiers during BCT. Using qualitative focus group methods, study two assessed soldiers' perceptions about their sleep, and consequences of sleep disruption during BCT at Fort Jackson in Columbia, SC. …

Mass Spectrometry And Tandem Mass Spectrometry Characterization Of Protein Patterns, Protein Markers And Whole Proteomes For Pathogenic Bacteria, Jennifer Intelicato-Young

Theses and Dissertations

There have been many recent reviews published on MALDI-TOF MS (matrix assisted laser desorption/ionization time-of-flight) MS (mass spectrometry) for identification of bacteria particularly with relevance to clinical microbiology. MALDI-TOF MS is now a mature technique for bacterial identification with great promise. The purpose of this review is to put into perspective MALDI-TOF MS and other widely used mass spectrometry methods for characterization of proteins. MALDI-TOF MS is used for rapid determination of a mass pattern of proteins for bacterial characterization; these proteins

are generally not identified. Alternatively, after gel separation, MALDI TOF-TOF MSMS (tandem mass spectrometry) or on-line LC-ESI MS-MS …

A Double Blind Community-Based Randomized Trial Of Amoxicillin Versus Placebo For Fast Breathing Pneumonia In Children Aged 2-59 Months In Karachi, Pakistan (Retapp), Fyezah Jehan, Muhammad Imran Nisar, Salima Kerai, Nick Brown, Benazir Balouch, Zulfiqar Hyder, Gwen Ambler, Amy Sarah Ginsburg, Anita K. M. Zaidi

Department of Paediatrics and Child Health

Background: Fast breathing pneumonia is characterized by tachypnoea in the absence of danger signs and is mostly viral in etiology. Current guidelines recommend antibiotic therapy for all children with fast breathing pneumonia in resource limited settings, presuming that most pneumonia is bacterial. High quality clinical trial evidence to challenge or support the continued use of antibiotics, as recommended by the World Health Organization is lacking.

Methods/Design: This is a randomized double blinded placebo-controlled non-inferiority trial using parallel assignment with 1:1 allocation ratio, to be conducted in low income squatter settlements of urban Karachi, Pakistan. Children 2-59 months old with fast …

Subjective Health Status And Health-Related Quality Of Life Among Women With Recurrent Vulvovaginal Candidosis (Rvvc) In Europe And The Usa, Samuel Aballéa, Florent Guelfucci, Julian Wagner, Amine Khemiri, Jean-Paul Dietz, Jack Sobel, Mondher Toumi

Wayne State University Associated BioMed Central Scholarship

Abstract

Background

Recurrent vulvovaginal candidosis (RVVC) is a chronic condition causing discomfort and pain. Health status and health-related quality of life (HRQoL) in RVVC were never previously described using validated questionnaires. The objective of this study is to describe subjective health status and HRQoL and estimate health state utilities among women with RVVC.

Methods

A cross-sectional online survey was conducted among women who reported having suffered four or more yeast infections over the past 12 months, in five European countries (France, Germany, Italy, Spain and the UK) and the USA. Index scores were derived from the EQ-5D, a questionnaire providing …

Clinical Outcomes In Patients With Heterogeneous Vancomycin-Intermediate Staphylococcus Aureus Bloodstream Infection, Anthony M. Casapao, N. Leonard, Susan L. Daivs, Thomas P. Lodise, Nimish Patel, Debra A. Goff, Kerry L. Laplante, Brian A. Potoski, Michael J. Rybak

Pharmacy Practice and Clinical Research Faculty Publications

The prevalence of heterogeneous vancomycin-intermediate Staphylococcus aureus (hVISA) infections varies in the literature, a problem complicated by the lack of routine screening procedures; however, limited data suggest that hVISA has been associated with persistent bloodstream infections (BSI) and vancomycin failure, yet these studies have been confounded by design issues. We conducted this study to compare the characteristics of patients with BSI caused by hVISA with those with vancomycin-susceptible Staphylococcus aureus (VSSA) treated with vancomycin. This retrospective, multicenter matched (1:1) cohort study compared the clinical characteristics and outcomes of hVISA and VSSA. Patients with hVISA methicillin-resistant Staphylococcus aureus (MRSA) BSI from …

Optimization Of Labeling Techniques; Determination Of Best Parameter For Olfactory Mucosal Progenitor Cell Delivery And Study Of Effects Of Methylene Blue And Polyethylene Glycol In An Animal Model Of Spinal Cord Injury, Kiran Kumar Rokkappanavar

Wayne State University Theses

In the United States, the incidence of TSCI is about 40 per million persons per year, with approximately 250,000 living survivors of TSCI in the United States in July 2005. A number of human clinical trials (85) are ongoing using stem cells to evaluate the methods to reduce the injury following TBI. Among the stem cells, olfactory mucosal progenitor cells have several advantages with respect to ease of obtaining, fate of cells, etc. Our study was intended to optimize the labeling technique and to determine the best parameter to deliver the stem cells to the site of injury. We found …

Effects Of Phase Feeding Different Levels Of Fungus Myceliated Grain On Broiler Performance And Health, Joi N. Jackson

Theses

A study was conducted to evaluate the effect of phase feeding a combination of 3 different medicinal mushrooms (Lentinus edodes, Ganoderma lucidum, Pleurotus ostreatus) via Fungus Myceliated Grain (FMG) on broiler performance and health after natural and experimental exposure to field strains of Eimeria. A total of 300, day-of-hatch, female broiler chickens were weighed and randomly assigned into the following dietary treatment conditions: 1) 1 % starter, 2) 1 % grower, 3) 1 % starter-grower-finisher, 4) 5% starter, 5) 5% grower, 6) 5% starter grower-finisher, 7) 10% starter, 8) 10% grower and 9) 10% starter-grower-finisher. Each treatment was replicated 3 …

The Ethical Balance Between Individual And Population Health Interests To Effectively Manage Pandemics And Epidemics, John Mary Mooka Kamweri

Electronic Theses and Dissertations

There is no overlapping criterion providing a basis for attaining balance between individual and population oriented ethical concerns generated in the pandemic and the epidemic interventions. The shortfall leads to competing individual and population interests that hamper the effective management of pandemics and epidemics. The libertarian model focuses on advancing individual rights. The epidemiological model focuses upon population health. The social justice model focuses on a broader perspective than individual rights and population health to include universal human rights.

This dissertation suggests a Mixed Interests Ethics Model (MIEM) to ethically negotiate a balance between the individual and population interests in …

Frequency, Patterns, And Preferences Of Lubricant Use During Anal Intercourse Within Male Sexual Partnerships In Lima, Peru: Implications For A Rectal Microbicide Hiv Prevention Intervention, Jesse L. Clark, Hector Salvatierra, Eddy R. Segura, Ximena Salazar, Kelika Konda, Jerome T. Galea, Jeffrey D. Klausner, Thomas J. Coates, Carlos F. Caceres

Social Work Faculty Publications

Understanding current practices of lubricant use during anal intercourse can help to assess the contexts for the introduction of topical rectal microbicides as an HIV prevention tool for men who have sex with men (MSM). We used quantitative and qualitative methods to assess: current patterns of lubricant use; preferred characteristics of commercial lubricant formulations; and social and behavioral contexts of lubricant use within male sexual partnerships in Lima, Peru. Between 2007 and 2008, we conducted a quantitative behavioral survey with 547 MSM followed by qualitative individual and group interviews with 36 MSM from Lima, Peru. Approximately half of all participants …

New Perspectives On How To Discover Drugs From Herbal Medicines: Cam's Outstanding Contribution To Modern Therapeutics, Si-Yuan Pan, Shu-Feng Zhou, Si-Hua Gao, Zhi-Ling Yu, Shuo-Feng Zhang, Min-Ke Tang, Jian-Ning Sun, Dik-Lung Ma, Yi-Fan Han, Wang-Fun Fong, Kam-Ming Ko

Pharmacy Faculty Publications

With tens of thousands of plant species on earth, we are endowed with an enormous wealth of medicinal remedies from Mother Nature. Natural products and their derivatives represent more than 50% of all the drugs in modern therapeutics. Because of the low success rate and huge capital investment need, the research and development of conventional drugs are very costly and difficult. Over the past few decades, researchers have focused on drug discovery from herbal medicines or botanical sources, an important group of complementary and alternative medicine (CAM) therapy. With a long history of herbal usage for the clinical management of …

Kony2012: The New Face Of Citizen Engagement, Nicole Audette

Exchange: The Journal of Public Diplomacy

This paper breaks down the nongovernmental components of public diplomacy and examines the evolution of new public diplomacy. It discusses the rise of nonstate actors and the importance they play in changing international diplomatic engagements. It explores the rising prominence of both soft power and public relations on the global stage, highlighting the importance of strategic relationships and effective communications. The case study, used to exemplify the power of new public diplomacy, examines how Invisible Children’s KONY2012 campaign used public diplomacy to harness successfully the power of the American people to influence governmental foreign policy. The author shows that Invisible …

A Single-Parameter Model Of The Immune Response To Bacterial Invasion, Lester Caudill

Department of Math & Statistics Faculty Publications

The human immune response to bacterial pathogens is a remarkably complex process, involving many different cell types, chemical signals, and extensive lines of communication. Mathematical models of this system have become increasingly high-dimensional and complicated, as researchers seek to capture many of the major dynamics. In this paper, we argue that, in some important instances, preference should be given to low-dimensional models of immune response, as opposed to their high-dimensional counterparts. One such model is analyzed and shown to reflect many of the key phenomenological properties of the immune response in humans. Notably, this model includes a single parameter values, …

Life Without Hope? Huntington's Disease And Genetic Futurity, Gerry Canavan

English Faculty Research and Publications

No abstract provided.

Is The Reporting Timeliness Gap For Avian Flu And H1n1 Outbreaks In Global Health Surveillance Systems Associated With Country Transparency?, Feng-Jen Tsai, Eva Tseng, Chang-Chuan Chan, Hiko Tamashiro, Sandrine Motamed, André C Rougemont

Division of Internal Medicine Faculty Papers & Presentations

BACKGROUND: This study aims to evaluate the length of time elapsed between reports of the same incidents related to avian flu and H1N1 outbreaks published by the WHO and ProMED-mail, the two major global health surveillance systems, before and after the amendment of the International Health Regulations in 2005 (IHR 2005) and to explore the association between country transparency and this timeliness gap.

METHODS: We recorded the initial release dates of each report related to avian flu or H1N1 listed on the WHO Disease Outbreak News site and the matching outbreak report from ProMED-mail, a non-governmental program for monitoring emerging …

Correctional Telemedicine And Its Benefits For Female Inmates, Chelsea Oliver

Applied Research Projects

The purpose of this study is to identify health issues that are specific to the female population of correctional institutions and how telemedicine would be beneficial in providing healthcare to same. A questionnaire was developed using information from literature on the topics obtained from a variety of places. Once the questionnaire was completed, a randomly selected group of telemedicine providers received the questionnaire, along with an introduction and information on the study that was being conducted.

The barriers of this study came from telemedicine providers either not responding to the questionnaire entirely or the providers only being able to answer …

Parasites In Paraguay: An Analysis Of The Presentation And Management Of Intestinal Parasites By Short-Term Medical Mission Volunteers In Paraguay, Robert A. Eick

Master of Public Health Program Student Publications

Introduction. International medical aid in the form of short-term medical missions (STTMs) receives more than $250 million in annual donations. Needs assessments prior to the STTM and outcomes analysis for improvement are often lacking. Objective. Describe the patient population attended to during a one-week STTM in Paraguay, South America, while examining “parasites” as a presenting concern, as a diagnosis by the providers, and the prescription of antiparasitic treatment. This analysis identified variance in intestinal parasite presentation and management by age, gender, and location. The administration frequency of antiparasitic medication was considered as prophylactic versus disease-treatment based.

Methods. This study was …

A Case For Gun Violence Prevention In Medical And Public Health Curriculum, Shaun Hamilton

Master of Public Health Program Student Publications

Gun violence profoundly impacts the landscape of violence in the United States. The costs of gun injuries are, estimated to be $100 billion each year in the United States. Although many physicians and health professionals agree that they have a role to play in the prevention of violence (American Academy of Pediatrics [AAP], 2002), there is a distinct disconnect between perceived responsibility and actual practice (Sidelinger, Guerrero, Rodriguez-Frau, & Mirabal-Colon, 2005). Physicians inherently have a role to play in the prevention of violence. The nature of their occupation places them in the “process” of violence in the form of treatment. …

The Scientific Evidence Validating The Use Of Honey As A Medicinal Agent, Raitzel Chemda Bernstein

The Science Journal of the Lander College of Arts and Sciences

Honey has been used to treat wounds, infections, and other diseases since ancient times. Due to the widespread bacterial resistance to antibiotics, scientists have investigated the healing power of honey. Numerous studies have documented the broad-spectrum antibacterial effect of honey and its success in inhibiting MRSA. The use of honey as a wound dressing has proven to successfully heal chronic wounds in short amounts of time by inhibiting pathogens, reducing inflammation, rebuilding damaged tissue, and minimizing scarring. The mechanisms of action are directly related to the high hydrogen peroxide and rich polyphenol content present in honey. These two components account …