Digital Commons Network^™

The Leadership Act And Its Policy Requirement: Changing Laws, Not Reality, Ami S. Watkin

Brooklyn Law Review

No abstract provided.

Free Trade And Free Immigration: Why Domestic Competitive Injury Should Never Influence Government Policy, Richard A. Epstein

University of Chicago Law Review

No abstract provided.

Nitric Oxide As A Mediator Of Hookworm Infection Ancylostoma Ceylanicum, Amanda Berndt

Undergraduate Honors Theses

Hookworm infection is a major cause of anemia, malnutrition, growth delay and cognitive defects in resource poor countries. Human and animal studies suggest that infection with these intestinal nematodes is associated with impaired cellular immunity, characterized by reduced lymphocyte proliferation in response to both parasite and heterologous antigens. In vitro studies have shown that nitric oxide (NO) is one of the leading agents causing impaired cellular responses, Spleenocytes from infected hamsters secreted more NO in culture than did those from naive animals. In order to further identify the role of NO in hookworm pathogenesis and pathology, we conducted an experiment …

Bacterial Colonization And Extinction On Marine Aggregates: Stochastic Model Of Species Presence And Abundance, Andrew M. Kramer, M. Maille Lyons, Fred C. Dobbs, John M. Drake

Integrative Biology Faculty and Staff Publications

Organic aggregates provide a favorable habitat for aquatic microbes, are efficiently filtered by shellfish, and may play a major role in the dynamics of aquatic pathogens. Quantifying this role requires understanding how pathogen abundance in the water and aggregate size interact to determine the presence and abundance of pathogen cells on individual aggregates. We build upon current understanding of the dynamics of bacteria and bacterial grazers on aggregates to develop a model for the dynamics of a bacterial pathogen species. The model accounts for the importance of stochasticity and the balance between colonization and extinction. Simulation results suggest that while …

Comparison Of Th1 Cytokines And T Cell Markers Gene Expressions Between Virulent And An Attenuated Eiav Vaccine Strain, Talia R. Henkle

Lewis Honors College Capstone Collection

The equine infectious anemia virus (EIAV) is closely related to HIV and has been used as a model to identify protective mechanisms against lentivirus infection. In horses, EIA infection progresses for about a year before infected horses manage to control virus replication. This naturally-gained protection is absolutely dependent on active immune responses as evidenced by the fact that immunosuppressive drugs can induce the recurrence of disease. As the resolution of initial viremia correlates with the appearance of virus specific cytotoxic T lymphocytes (CTL), we believe that cellular immune responses play a key role in controlling EIAV in the horse. In …

Human Behavioral And Ecological Risk Factors For Lyme Disease Infection On Block Island, Rhode Island, Casey James Finch

Public Health Theses

Peridomestic exposure to infected Ixodes scapularis nymphs is considered the dominant means of infection with tick-borne pathogens in the eastern United States. Previous studies of risk of developing tick-borne infection established a positive association between the density of infected nymphs and Lyme disease cases at the population level. Studies examining the effectiveness of personal protective behaviors have not included measures of tick exposure. This study simultaneously assesses the effect of tick exposure and human behavior in Lyme disease infection risk using a longitudinal serosurvey study on Block Island, RI. Tick exposure risk at all Island properties was estimated by identifying …

Geographic Variation In The Costs Of Prostate Cancer Care, Avantika Arun Saraf

Public Health Theses

Objectives: 1) To review costs of prostate cancer care in multiple countries 2) To summarize studies assessing regional variation in costs of prostate cancer care within the United States 3) To characterize variation across hospital referral regions (HRRs) in Medicare expenditures on initial prostate cancer care and evaluate its determinants. Methods: A thorough literature search was conducted to identify the costs of prostate cancer care in different countries and within the United States (to assess regional variation in costs). Subsequently, the thesis involved an in-depth use of the Surveillance, Epidemiology and End Results (SEER)-Medicare linked database and the Medicare 5% …

Doc 2013-01 Master Of Physician Assistant Practice (Mpap) Program, University Of Dayton. School Of Education And Allied Professions

Senate Documents

Constitution of the Academic Senate of the University of Dayton, Academic Senate DOC 11-04

Environmental Factors Influencing Epidemic Cholera, Antarpreet Jutla, Elizabeth Whitcombe, Nur Hasan, Bradd Haley, Ali S. Akanda, Anwar Hug, Munir Alam, R. Bradley Sack, Rita Colwell

Civil & Environmental Engineering Faculty Publications

Cholera outbreak following the earthquake of 2010 in Haiti has reaffirmed that the disease is a major public health threat. Vibrio cholerae is autochthonous to aquatic environment, hence, it cannot be eradicated but hydroclimatology-based prediction and prevention is an achievable goal. Using data from the 1800s, we describe uniqueness in seasonality and mechanism of occurrence of cholera in the epidemic regions of Asia and Latin America. Epidemic regions are located near regional rivers and are characterized by sporadic outbreaks, which are likely to be initiated during episodes of prevailing warm air temperature with low river flows, creating favorable environmental conditions …

Nwrc Annual Publications List – 2013

United States Department of Agriculture Wildlife Services: Staff Publications

No abstract provided.

New World Bats Harbor Diverse Influenza A Viruses, Suxiang Tong, Xueyong Zhu, Yan Li, Mang Shi, Jing Zhang, Melissa Bourgeois, Hua Yang, Xianfeng Chen, Sergio Recuenco, Jorge Gomez, Li-Mei Chen, Adam Johnson, Ying Tao, Cyrille Dreyfus, Wenli Yu, Ryan Mcbride, Paul J. Carney, Amy T. Gilbert, Jessie Chang, Zhu Guo, Charles T. Davis, James C. Paulson, James Stevens, Charles E. Rupprecht, Edward C. Holmes, Ian A. Wilson, Ruben O. Donis

United States Department of Agriculture Wildlife Services: Staff Publications

Aquatic birds harbor diverse influenza A viruses and are a major viral reservoir in nature. The recent discovery of influenza viruses of a new H17N10 subtype in Central American fruit bats suggests that other New World species may similarly carry divergent influenza viruses. Using consensus degenerate RT-PCR, we identified a novel influenza A virus, designated as H18N11, in a flat-faced fruit bat (Artibeus planirostris) from Peru. Serologic studies with the recombinant H18 protein indicated that several Peruvian bat species were infected by this virus. Phylogenetic analyses demonstrate that, in some gene segments, New World bats harbor more influenza …

Spatial Ecology Of Urban Raccoons In Northeastern Ohio: Implications For Oral Rabies Vaccination, Are R. Berentsen, Mike R. Dunbar, Chadd E. Fitzpatrick, W. David Walter

United States Department of Agriculture Wildlife Services: Staff Publications

In 1977, rabies was detected in a raccoon (Procyon lotor) in West Virginia, and since the mid-1980s raccoon variant rabies has spread throughout the eastern United States and moved west as far as the eastern edge of Cleveland, Ohio. The primary tool to combat this spread is the distribution of oral rabies vaccine (ORV) baits. A thorough knowledge of raccoon space use is critical in determining bait placement, particularly in urban areas. We monitored nine raccoons in urban areas of Cleveland, Ohio, calculated home range sizes, monitored raccoon movement with respect to potential movement barriers, and used resource …

The Real Issue Behind Stanford V. Roche: Faulty Conceptions Of University Assignment Policies Stemming From The 1947 Biddle Report, Sean M. O'Connor

Michigan Telecommunications & Technology Law Review

The recent Supreme Court decision in Stanford v. Roche laid bare a faulty assumption of the federal research funding system. Government patent policy for federally funded research relies on "contractors"--the recipients of federal funding--to secure patent assignments from their employees. While this practice was routine for private firms and nonprofit research institutions, it was not for universities. This was in part based on the relationship of faculty and other researchers to universities that differed from industry employment relationships. The roots of this faulty assumption can be traced to the seminal 1947 Biddle Report. Detailed monographs drafted as appendices to the …

The Convention On The Rights Of Persons With Disabilities: Reflection On Four Flaws That Tarnish Its Promise, Lucia A. Silecchia

Scholarly Articles

On December 13, 2006, the United Nations adopted the Convention on the Rights of Persons with Disabilities (“CRPD”). Widely touted as the “first comprehensive human rights treaty of the 21st century,” and effusively praised for its open negotiation process, the CRPD was opened for signature on March 30, 2007. The CRPD quickly entered into force on May 3, 2008. As it rapidly amassed signatories, the CRPD inspired great hope that its comprehensive approach would do much to overcome the consistent failure to promote the dignity of those with disabilities in meaningfully concrete ways.

The CRPD has garnered much recent and …

Spirituality, Faith, And Mild Alzheimer's Disease, Jocelyn Shealy Mcgee, Dennis R. Myers, Holly Carlson, Angela E. Pool-Funai, Paul A. Barclay

Sociology Faculty Publications

There is some evidence for a positive association between spirituality, cognitive, and behavioral functioning in people with Alzheimer’s disease (AD). However, to our knowledge there is no published data to date that provides an explanatory model for these findings. Twenty-eight individuals with mild AD received in-depth interviews and measures of cognitive, behavioral, emotional, and spiritual functioning to gain insight into this question in this mixed methods study. Findings revealed that people with mild AD can actively engage in meaningful discussion about how spirituality influences their experience of living with AD; that they remain deeply devoted to a relationship with the …

Study Of Glycation And Advanced Glycation On A Humanized Monoclonal Antibody, Hong Liu

Open Access Dissertations

In the presented work, we reviewed the protein glycation effect on recombinant monoclonal antibodies (MAbs), and demonstrated an in vitro glycation model on rhuMAb A was a successful tool to evaluate site-specific glycation. We also implemented this model to study MAb glycation in three aspects: the formation of glycation, the change of glycation adducts under thermal stress, and the control of glycation adducts stability by formulation compositions.

A protein characterization study demonstrated that the in vitro forced glycation model generated seven glycated amino acid sites on rhuMAb A. The lysine residue K49 was the preferably site, while others sites were …

There's A Pattern Here: The Case To Integrate Environmental Security Into Homeland Security Strategy, James D. Ramsay, Terrence M. O'Sullivan

Security Studies & International Affairs - Daytona Beach

The time is long overdue to acknowledge that global climate and resource stresses, encompassed by the concept of environmental security (ES), are an increasingly important part of "homeland" security (HS) study and practice, by even the most restricted definitions of HS. Environmental security issues will affect global economic and political stability, US national interests, and the risk of war and terrorism. Just as homeland security encompasses many complex issues and interconnected subfields, environmental security (ES) is interdisciplinary by nature. In essence, ES is an emergent discipline borrowing from a combination of environmental studies — which decades ago integrated environmental science …

2013 Ipm Annual Report—Responding To Women’S Needs: Developing Hiv And Multipurpose Prevention Products, International Partnership For Microbicides

International Partnership for Microbicides (IPM)

No abstract provided.

Examining Kentucky Teachers' Encounters With Grieving Students: A Mixed Methods Study, Ashley M. Candelaria

Theses and Dissertations--Educational, School, and Counseling Psychology

The research on bereaved children’s outcomes is mixed, with the course of grief in young people prone to variation. Each child’s reaction following a loss is unique and should be considered in relation to psychological, academic, familial, social, environmental, and a number of additional factors. However, what is known is the important role that a supportive environment, stable adult figures, and early intervention may have for grieving youth. Teachers could be considered the first line of defense in identifying youth who are struggling with a loss, as they are significant adults with whom students have consistent contact on a daily …

Why Some Physicians Accept Healthcare It And Others Resist It: An Activity-Theoretic Analysis., Anol Bhattacherjee, Christopher Davis, Neset Hikmet

USF St. Petersburg Campus Faculty Publications

This paper examines why physicians react differently to the introduction of healthcare information technology (IT) in the same hospital at the same time. These diverse reactions, manifested as different forms of acceptance and resistance, are interpreted within the social-historical context of physicians’ work at a large urban hospital in the USA, using Activity Theory as the theoretical lens. While prior IT research has examined patterns of “similarities” in user behaviors, we examine user “differences” and reconcile these differences within a meta-theoretic framework. We also extend current IT usage research from voluntary to mandatory setting and demonstrate the viability of activity …

Persistent Human Herpesvirus-6 Infection In Patients With An Inherited Form Of The Virus, Shara N. Pantry, Maria M. Medveczky, Jesse H. Arbuckle, Janos Luka, Jose G. Montoya, Jianhong Hu, Rolf Renne, Daniel Peterson, Joshua C. Pritchett, Dharam V. Ablashi, Peter G. Medveczky

Molecular Medicine Faculty Publications

Human herpesvirus-6 (HHV-6)A and 6B are ubiquitous betaherpesviruses viruses with lymphotropic and neurotropic potential. As reported earlier, these viruses establish latency by integration into the telomeres of host chromosomes. Chromosomally integrated HHV-6 (CIHHV-6) can be transmitted vertically from parent to child. Some CIHHV-6 patients are suffering from neurological symptoms, while others remain asymptomatic. Four patients with CIHHV-6 and CNS dysfunction were treated with valganciclovir or foscarnet. HHV-6 replication was detected by reverse transcriptase polymerase chain reaction amplification of a late envelope glycoprotein. In this study we also compared the inherited and persistent HHV-6 viruses by DNA sequencing. The prevalence of …

Looking At Microbial Metabolism By High-Resolution (2)H-Nmr Spectroscopy, Victor P. Kutyshenko, Peter M. Beskaravayny, Maxim V. Molchanov, Svetlana I. Paskevich, Dmitry A. Prokhorov, Vladimir N. Uversky

Molecular Medicine Faculty Publications

We analyzed the applicability of high-resolution (2)H-HMR spectroscopy for the analysis of microbe metabolism in samples of mitochondrion isolated from rat liver and from aqueous extracts of homogenates of rat liver and other organs and tissues in the presence of high D2O contents. Such analysis is possible due to the fast microbe adaptation to life in the heavy water. It is also shown that some enzymatic processes typical for the intact cells are preserved in the homogenized tissue preparations. The microbial and cellular metabolic processes can be differentiated via the strategic use of cell poisons and antibiotics.

2013 Accountability Report Usf Tampa, Usf

USF Accountability Reports

No abstract provided.

Molecular Architecture Of The Uncleaved Hiv-1 Envelope Glycoprotein Trimer, Youdong Mao, Luis Castillo-Menendez, Liping Wang, Christopher Gu, Alon Herschhorn, Anik Désormeaux, Andrés Finzi, Shi-Hua Xiang, Joseph Sodroski

Nebraska Center for Virology: Faculty Publications

The human immunodeficiency virus (HIV-1) envelope glycoprotein (Env) trimer, a membrane-fusing machine, mediates virus entry into host cells and is the sole virusspecific target for neutralizing antibodies. Binding the receptors, CD4 and CCR5/CXCR4, triggers Env conformational changes from the metastable unliganded state to the fusion-active state. We used cryo-electron microscopy to obtain a 6-Å structure of the membranebound, heavily glycosylated HIV-1 Env trimer in its uncleaved and unliganded state. The spatial organization of secondary structure elements reveals that the unliganded conformations of both gp120 and gp41 subunits differ from those induced by receptor binding. The gp120 trimer association domains, which …

Variations In The Biological Functions Of Hiv-1 Clade C Envelope In A Shiv-Infected Rhesus Macaque During Disease Progression, For Yue Tso, Levon Abrahamyan, Shiu-Lok Hu, Ruth M. Ruprecht, Charles Wood

Nebraska Center for Virology: Faculty Publications

A better understanding of how the biological functions of the HIV-1 envelope (Env) changes during disease progression may aid the design of an efficacious anti-HIV-1 vaccine. Although studies from patient had provided some insights on this issue, the differences in the study cohorts and methodology had make it difficult to reach a consensus of the variations in the HIV-1 Env functions during disease progression. To this end, an animal model that can be infected under controlled environment and reflect the disease course of HIV-1 infection in human will be beneficial. Such an animal model was previously demonstrated by the infection …

Kaposi’S Sarcoma-Associated Herpesvirus Transactivator Rta Induces Cell Cycle Arrest In G0/G1 Phase By Stabilizing And Promoting Nuclear Localization Of P27Kip, Pankaj Kumar, Charles Wood

Nebraska Center for Virology: Faculty Publications

The Kaposi’s sarcoma-associated herpesvirus (KSHV) immediate-early gene, replication, and transcription activator (KRta) is a key viral protein that serves as the master regulator for viral lytic replication. In this study, we investigated the role of K-Rta in cell cycle regulation and found that the expression of K-Rta in doxycycline (Dox)-inducible BJAB cells induced cell cycle arrest in G₀/G₁ phase. Western blot analysis of key cell cycle regulators revealed that K-Rta-mediated cell cycle arrest was associated with a decrease in cyclin A and phosphorylated Rb (pS807/pS811) protein levels, both markers of S phase progression, and an increase in …

Low-Abundance Resistant Mutations In Hiv-1 Subtype C Antiretroviral Therapy-Naïve Individuals As Revealed By Pyrosequencing, Sandra Gonzalez, Damien C. Tully, Clement Gondwe, Charles Wood

Nebraska Center for Virology: Faculty Publications

Given the recent scale-up of antiretroviral therapy (ART) in sub-Saharan Africa, we sought to determine how often and at what levels do drug-resistant mutant variants exist in ART-naïve HIV subtype C infected individuals. Samples from 10 ART-naïve Zambian individuals were subjected to ultra-deep pyrosequencing (UDPS) to characterize the frequency of low-abundance drug resistance mutations in the pol gene. Low-abundance clinically relevant variants were detected for nucleoside reverse transcriptase inhibitors (NRTIs), non-nucleoside reverse transcriptase inhibitors (NNRTIs) and protease inhibitors (PIs) in eight of the ten subjects. Intermediate to high-level resistance was predicted for the majority of NRTIs. Mutations conferring resistance to …

Clade C Hiv-1 Isolates Circulating In Southern Africa Exhibit A Greater Frequency Of Dicysteine Motif-Containing Tat Variants Than Those In Southeast Asia And Cause Increased Neurovirulence, Vasudev R. Rao, Ujjwal Neogi, Joshua S, Talboom, Ligia Padilla, Mustafizur Rahman, Cari Fritz-French, Sandra Gonzalez-Ramirez, Anjali Verma, Charles Wood, Ruth M. Ruprecht, Udaykumar Ranga, Tasnim Azim, Vinayaka R. Prasad

Nebraska Center for Virology: Faculty Publications

Background: HIV-1 Clade C (Subtype C; HIV-1C) is responsible for greater than 50% of infections worldwide. Unlike clade B HIV-1 (Subtype B; HIV-1B), which is known to cause HIV associated dementia (HAD) in approximately 15% to 30% of the infected individuals, HIV-1C has been linked with lower prevalence of HAD (0 to 6%) in India and Ethiopia. However, recent studies report a higher prevalence of HAD in South Africa, Zambia and Botswana, where HIV-1C infections predominate. Therefore, we examined whether Southern African HIV-1C is genetically distinct and investigated its neurovirulence. HIV-1 Tat protein is a viral determinant of neurocognitive dysfunction. …

Global Neuroaids Roundtable, Jeymohan Joseph, Cristian L. Achim, Michael J. Boivin, Bruce J. Brew, David B. Clifford, Deborah A. Colosi, Ronald J. Ellis, Robert K. Heaton, Amadou Gallo-Diop, Igor Grant, Georgette D. Kanmogne, Mahendra Kumar, Scott Letendre, Thomas D. Marcotte, Avindra Nath, Carlos A. Pardo, Robert H. Paul, Lynn Pulliam, Kevin Robertson, Walter Royal Iii, Ned Sacktor, Pasiri Sithinamsuwan, Davey M. Smith, Victor Valcour, Brian Wigdahl, Charles Wood

Nebraska Center for Virology: Faculty Publications

In May 2012, the Division of AIDS Research at the National Institute of Mental Health (NIMH) organized the “Global NeuroAIDS Roundtable” in conjunction with the 11th International Symposium on Neurovirology and the 2012 Conference on HIV in the Nervous System. The meeting was held in New York, NY, USA and brought together NIMH-funded investigators who are currently working on projects related to the neurological complications of AIDS (NeuroAIDS) in Africa, Asia, Eastern Europe, and Latin America in order to provide an opportunity to share their recent findings and discuss the challenges encountered within each country. The major goals of the …

Extracellular Proteases Are Key Mediators Of Staphylococcus Aureus Virulence Via The Global Modulation Of Virulence-Determinant Stability, Stacey L. Kolar, J. Antonio Ibarra, Frances E. Rivera, Joe M. Mootz, Jessica E. Davenport, Stanley M. Stevens Jr., Alexander R. Horswill, Lindsey N. Shaw

Molecular Biosciences Faculty Publications

Staphylococcus aureus is a highly virulent and successful pathogen that causes a diverse array of diseases. Recently, an increase of severe infections in healthy subjects has been observed, caused by community-associated methicillin-resistant S. aureus (CA-MRSA). The reason for enhanced CA-MRSA virulence is unclear; however, work suggests that it results from hypersecretion of agr-regulated toxins, including secreted proteases. In this study, we explore the contribution of exo-proteases to CA-MRSA pathogenesis using a mutant lacking all 10 enzymes. We show that they are required for growth in peptide-rich environments, serum, in the presence of antimicrobial peptides (AMPs), and in human blood. …