Digital Commons Network^™

The Use Of Fima Gene Primers For Detection Of Salmonella Spp. Isolated From Children With Diarrhea, Alaa Abdel-Kadhim Jawad, Adnan Hamad Al-Hamadani, Nasma N. Al-Hajia, Aws Rassul Hussain Al-Salih

Al-Qadisiyah Medical Journal

A total of 480 fecal samples were collected from children (less than 3 years old) , of both sexes suffering from diarrhea who admitted to The Teaching Hospital of Maternity and Pediatrics in Al- Diwaniya governorate. Salmonella spp. were isolated and identified using bacterial culturing on selective media, in addition to, biochemical and Mini API 20E. Polymerase chain reaction (PCR) was used to detect fimA gene encoding for biosynthesis of fimA of Salmonella spp.   The results revealed that the rate of Salmonella isolates in fecal samples of patients were (38/480) 7.9% using cultural and MiniAPI20 E, when the PCR technique …

Detection Of Causative Agents, Antifungal Susceptibility Profile And Cross-Resistance Of Candida Albicans Isolated From Oral And Vaginal Candidiasis, Noor Ismaeel Nassir Al-Rubaye, Adnan H.Al-Hamadani, Hammady Al- Hilaly

Al-Qadisiyah Medical Journal

This study was designed to investigate antifungal susceptibility and cross resistance of C. albicans isolates toward four antifungal drugs; Amphotericin B, nystatin, fluconazole and ketoconazole The study groups included 208 patients( 108 women and 100 children) who attented the Al-Suwayra Hospital/ Kut Province during the period from December 2008 to April 2009. Patients groups were represented by women who were complaining of vaginal discharge and itching and children who were complaining of oral thrush and infected with different type of disease include upper and lower respiratory tract infection, gastroenteritis, kala-azar and septicemia. The study showed that the infection percentage of …

The Use Of Omp W Gene In Detection Of Vibrio Cholerae Isolated From Diarrhea Cases Of Children In Al-Diwaniya Province, Thuraya Aamer Habeeb, Adnan H. Al-Hamadani, Jawad K. Al-Janabi

Al-Qadisiyah Medical Journal

A total of 221 stool samples were collected from children suffering from watery diarrhea, less than 15 years old of both genders whom admitted to the Maternity and Children Teaching Hospital in Al- Diwaniya Province, In addition to that, 27 water samples were also collected from three different loci of ALDiwaniya River, at the period of October 2008 to June 2009,in order to evaluate the routine laboratory diagnostic procedures in the diagnosis the multi-serogroups or serotype of Vibrio cholerae strains and compared them with molecular technique as Polymerase chain reaction (PCR).Vibrio cholerae has been isolated and identified by using culturing …

Extended Spectrum Ãÿ-Lactamase (Esbl) Mediated Resistance To Third Generation Cephalosporins In Urinary Tract Infection Isolates Of Klebseilla Pneumonia, Ali Muhsin Almohana, Zainab Jabir Hadi, Suad Abdul- Hadi

Al-Qadisiyah Medical Journal

Objective: To examine the dissemination of extended spectrum ßlactamase (ESBL) producing isolates of Klebseilla pneumoniae obtained from patients with significant bacteriuria.Methods: ESBL and multiantibiotic resistance were studied in a total of 25 isolates of K. pneumoniae.Results: all isolates found resistance to at least five of the antibiotics used in this study. All the isolates were found sensitive to the antibiotic imipenem. 5 (22.7%) of these isolates were ESBL producing. The ESBL producing isolates were more resistant to antibiotics than non-ESBL isolates. The ESBL activity could be experimentally transferred to recipient Escherichia coli MM294.Conclusions: present study has found the occurrence of …

In Vitro Susceptibility Of Leishmania Donovani Promastigotes To Some Antimicrobials Compared With Meglumine Antimoniate., May A. Omran, Ali M. Jasim, Sundus N. Jasim, Saif J. Yasir

Al-Qadisiyah Medical Journal

This experimental study was designed to evaluate the effectivness of some antimicrobial agents against Leishmania donovani promastigotes (LDP) in vitro. It was performed in College of Medicine in Kufa University during october and november 2008. It included treatment of LDP, cultured in Roswell Park Memorial Institute (RPMI) medium enriched with 10% fetal calf serum (FCS) for 14 days, with azithromycin (6, 12, and 18 μg /ml), trimethoprimsulfamethoxasol (0.5-9.5 μg/ml), norfloxacin (1 μg /ml ) and meglomin antimoniate (20 μg/ml).The control group contained no antimicrobial with the LDP. Samples were grouped so that each group (20 samples) treated under standardized conditions …

Use Of Pcr Amplification For The Evaluation Of Tb Diagnosis In Children By Classic Methods, Yahia Abedalreda Abbas, Adnan H. Al-Hamdani

Al-Qadisiyah Medical Journal

There have been few studies evaluating the efficacy of polymerase chain reaction (PCR) testing in Front other routine test for diagnosis of TB in children. The tuberculin skin test (TST) used to detect Mycobacterium tuberculosis infection in children has many drawbacks, and a new diagnostic test for has been introduced. We assessed the diagnostic yield of PCR prospectively in a blinded study of patients referred to Al-Nassyria center for chest diseases and other private laboratories from January to June 2008 and compared the results to the routine TST, smears with Ziehl-Neelsen staining and culture on Lo¨wenstein-Jensen medium which used as …

Mucosal Immunology Of Human Infectious Diseases, Ibrahim M.S. Shnawa

Al-Qadisiyah Medical Journal

Mucosal surfaces play crucial role in immune defenses against human infection diseases. Such mucosal defenses are in a way or other related to systemic defenses mechanisms. A review of ten years works 1999-2009 is being presented. Mucosal infections induces, mucosal and systemic immune response. The systemic response mostly higher than mucosal . The patient herd immunity induces low, moderate and high immune patients, showing normal distribution curves.

Using Pcr Technque In Comparison With Other Tests In The Diagnosis Of Pulmonary Tb Associated With Mycotic Infections In Al-Qadisiya Province., Adnan H. Al-Hamadani, Jaafar K. Al-Mousawi, Orass M.Al-Taee

Al-Qadisiyah Medical Journal

Background: Tuberculosis (TB) remains an important public health problem world wide. It is one of the leading infectious diseases in the world and is responsible for more than 3 million deaths and 8 million new cases annually.The report of World Health Organization (WHO) estimated, for the year 2002 a global incidence of 8.8 million new cases, including 3.9 million smear-positivesubjects.Aim of the study: We aimed to evaluate the effectiveness of available rapid diagnostic tests to identify TB infection.  The main goal of this study was to use the PCR technique for the rapid detection of tuberculosis using sputum sampling and …

Staphylococcus Aureus Nasal Carriage Among Medical Staff In Al-Nasiriyah Teaching Hospital ,Thi-Qar,Iraq., Dheaa K. Al-Omer, Hameed N.Al-Yassiri

Al-Qadisiyah Medical Journal

Background : nasal carrier of Staphylococcus aureus is a common health problem world wide.Objective: The present study was conducted to assess the epidemiology of Staphylococcus aureus nasal carriers among hospital staff in AN- Nasiriyah teaching Hospital ,Thi-Qar ,Iraq. Patients and methods: Nasal swabs were taken from each of 140 personnel. After culturing on blood agar for overnight, probablestaphylococcal isolates were identified and subjected to tube Coagulase test. After a two-week interval, second nasal swabs were taken from the subjects whose first cultures were positive for S. aureus.Results: Nasal carriage was defined in 31 (22.14%) personnel with positive culture for both …

Ceftriaxone Therapy Vs. Ciprofloxacin In Treatment Of Typhoid Fever In Adult Patients., Radhi F.Alshaibani

Al-Qadisiyah Medical Journal

Patients & method: 200 patients attending to the fever consultation unit from January 2008 to March 2009 in Al-dewania teaching hospital diagnosed on the base of clinical and rising widal O titer as typhoid fever patient were randomly equally divided into two groups G1 received ceftriaxone 1gm TID and G2 received ciprofloxacin 500 mg BID, every patient was closely followed by temperature chart for 7 days.Result: 100% of G1 patients were afebrile (5) days after commencement of treatment while only 50% of G2 patients became afebrile (7) days after treatment.Conclusion: this probably warning that resistant for ciprofloxacin is an evolving …

Detection Of Anti-Rubella Virus Igm And Igg In Aborative Pregnant Women In Al-Qadisiya Governorate, Ja'afar K. Nama, Yuniss A. Al-Khfaji, Saif J. Yasir

Al-Qadisiyah Medical Journal

The present study aimed to detect the levels of IgM and IgG immunoglobulins in their sera. The study included a collection of venous blood samples from three hundred women underwent abortion ranging in age from (15-35) years from Al Qadisiya governorate. Enzyme- Elinked immunosorbant assay (ELISA), was used to determine the immunological response against rubella virus in our the samples. ELISA test reflected a new infections which was (8 %) positive. Anti-rubella IgG antibodies ELISA test revealed (70%) positive result.

Taus Versus Tvus In Detection Early Pregnancy Complication, Amjaad Majeed Hameed

Al-Qadisiyah Medical Journal

Background:Using TVUS(transvaginal ultrasound ) visualization of pelvic organ is limited due to attenuation of ultrasound beam byintervening anterior abdominal wall and pelvic fat while TVUS (high frequency transducers 5-7MHz) now day regard important revolution in diagnosis of early pregnancy and their complications ,because it facilitate high resolution image of target organ uterus , adenaxa and cul de sac.Aim of study:To evaluate TVUS in detection of early pregnancy complication and compare transvaginal approach with TAUS . Patient and Methods :Sixty four patients were included in this study all of them have positive pregnancy test with signs and symptoms of early pregnancy …

Prevalence Of Hepatitis C Viral Infection Among Multitransfused Thalassemic Patients, A'Alan Hadi Al-Zamili, Abass M. Al-Shebani, Rahman Karim Mohsin

Al-Qadisiyah Medical Journal

A prospective study was conducted on 325 patients (187 males and 138 females) with B –thalassemia major &sickle- thalassemia - who were attending the thalassemia center at Maternity and Children Teaching Hospital in Al-Diwaniya , from the period of September 2007 till April 2008. Their ages ranged from 1-20 years. They were studied for Anti HCV sero positivity among patients with B- thalassemia major &Sickle- thalassemia (12 patients (4.01%) and 1 patient (3.84%) respectively). Anti HCV sero positivity was significantly higher among patient's ages from (5-15 years) and also sero positivity was more among males. Statistically, there were significant differences …

The Microorganism And Underlying Urological Anomalies Causing The Urinary Tract Infection In The Children Attended The Pediatric Surgery Clinic At The Maternity And Children Teaching Hospital Al-Qadisiya\ Iraq, Mohammed J. Aboud, Manal Mohammed Kadhim, Manal Mohammed Kadhim

Al-Qadisiyah Medical Journal

Urinary tract infections (UTI) are among the most common infections in children. UTI related morbidity remains high despite the use of numerous effective antibacterial agents . The sample of this study was conducted in the pediatric surgery unit at the Maternity and Children Teaching Hospital , Al-Diwaniya ( Iraq) and from the 1st of August 2005 to the end of July 2008. All the patients presenting with UTI to the outpatient clinic or to the surgical ward were investigated prospectively . The aim of this study was to determine how many patients had underlying urological abnormalities, the specific type of …

Evaluation Of Hepatitis B Virus Vaccination Among Children In Al-Diawynia City, Amina N. Al-Thwani, Nihad A.M. Al-Rashedi, Ali R. Omer

Al-Qadisiyah Medical Journal

This study attempts to determine the protective level of antibodies against hepatitis B surface antigen among vaccinated and unvaccinated children in Al-Diawynia city, age ranging 1- 10 year. A total of 220 blood samples have been collected and tested for detection of serological markers of hepatitis B virus (HBsAg, anti-HBs and anti- HBc). They included 150 children represented the vaccinated group and 70 children represented the unvaccinated group. Prevalence of anti-HBs was higher in vaccinated children 67.3% than in unvaccinated children 3.3%. Anti-HBc was not found in vaccinated children, while 4.2% in unvaccinated children. The distribution of protective level is …

The Implications Of Hla Phenotypes In Inflammatory Bowel Disease, Hummady A. Al-Hilaly, Khalida M. Al-Mousawy, Jassem Al-Khafaji, Nawal Al-Khalidy

Al-Qadisiyah Medical Journal

Susceptibility to Inflammatory Bowel Disease (IBD) is, in part, genetically determined, and the HLA genes are candidate for a role in the genetic susceptibility to this disease, because their products play a central role in the immune responses. Multiple studies have reported associations between HLA phenotypes and either Ulcerative Colitis (UC) or Crohn's Disease (CD), the major two disorders of IBD, but much of these data are still controversial. To estimate overall associations between HLA phenotypes and this disease, and to establish the probable etiologic or protective functions conferred by these molecules, a total of 71 Iraqis, Arabs patients (55 …

Study The Role Of Toxoplasma Gondii, Cytomegalovirus And Anti-Phospholipids Antibodies In Cases Of Abortion Among Women In Hilla City, Mohammed S. Al-Saeed, Mohammed A. Muhsin, Gayedaa J. Al-Juburi

Al-Qadisiyah Medical Journal

This study was conducted to detect the role of T.gondii and CMV and the possible association between them in cases of abortion. Also the role of auto antibodies against phospholipids (APL) and it's association with the mentioned parasite and virus among women who have consulted the-Children and Maternity Hospital and General Teaching Hospital in AL-Hilla city, during the period from November 2006 to April 2007. Out of 120 samples, 50(41.66%) were positive for anti-Toxoplasma antibodies. While out of 44 sera, 35(79.5%) and 8(18.8%) were positive for anti-CMV IgG and IgM antibodies respectively. All control samples have been found to be …

A Study About Bacterial Contamination In Maternity And Children Teaching Hospital, Beheeja A. Hmood, Adnan H. Al-Hamedany, Mohammad A. Al-Shammary

Al-Qadisiyah Medical Journal

The results of our study showed that percentage of bacterial contamination in operating rooms was (66.2%) before disinfection method, and after disinfection method was (47.5%). High contamination percentage (100%) was in room floor covers and was decreased to (60%) after disinfectant. The lowest percentage of contamination (1%) was in surgical instruments and disappeared after disinfectant. The percentage of contamination in disinfectants and antiseptics was (55%), high contamination percentage (75%) was in Chloroxylenol (Dettol) whereas the owest (25%) was in Formalin.

Epidemiology Of Hepatitis Hbv And Hcv At Thi-Qar Province €“ Iraq, Yahia Abdelreda Abass, Khawam R. Al-Husseiny, Aqeel Abass Kareem

Al-Qadisiyah Medical Journal

The study was conducted on 1990 patients [ 1140 (57.3%) males and 850 (42.7%) females] during three years 2005-2007 at Thi-Qar province. They suffering from sings and symptoms of liver diseases. The results improved no positive case of infection with HIV , and revealed 176 (8.8%) positive hepatitis cases. HBV had high prevalence than other types including 100(5%) cases with significant differences , followed by HCV 63(3.2%) and 13(0.7%)cases were recorded as dual infections (HBV+HCV). Significant increase of infections among males were recorded 113(64.2%) .High seroprevalence of HBV ,HCV and dual HBV+HCV were recorded in patients with age group 16-30 …

Gastroenteritis In Diwaniya, Fadhl Alzamili

Al-Qadisiyah Medical Journal

Diarrhoeal disease is an important cause of morbidity worldwide, and represents a leading cause of death especially in children,the majority of patients with acute Gastroenteritis do not seek medical advice and investigations are frequently omitted In those who do these investigations, therfore, there are no true records for this diseaseThis study aims to evaluate cases of gastroenteritis and effectiveness of therapy.Over (2) years period from January 2004-january 2006. evaluation of (318) patients With diarrhea were done in Diwaniya Teaching Hospital, who satisfied the following Inclusion criteria; (a) age > 16 y, (b) presented with acute gastroenteritis (C) treated with Or without …

Neonatal Septicemia In Al_Najaf Al_Ashref Governerate : Bacteriological Profile And Antimicrobial Sensitivity, Adnan H. Al-Hamadani, Adel H. Sheeh, Sinaa Abdulamir Al-Zubaidy

Al-Qadisiyah Medical Journal

Neonatal septicemia still one of the most important causes of neonatal sepsis and death. Knowledge of bacteriological profile and antibacterial sensitivity pattern in our locality play an important role in selection of antibacterial drug that will be very effective against causative pathogens.This study conducted in AL- NAJAF AL- ASHREF Governorate during period extended between Nov.2007 to Feb.-2008, about 57 clinically suspected cases of neonatal septicemia was enrolled, to study bacteriological profile and antibiotic sensitivity pattern. Results showed that 33 cases (58 %) gave positive blood culture results and there was no growth in 24 cases (42 %), from the positive …

Assessment Of Smart Check Recombinant Test In Diagnosis Of Pulmonary Tuberculosis In Al-Najaf Al-Ashraf Governorate, Abdul-Razzak Yassin Abdullah

Al-Qadisiyah Medical Journal

This cross sectional study was undertaken to evaluate the Smart Check Recombinant TB test which is a rapid membrane based screening test to detect the presence of antibodies to active M. tuberculosis (Serological test, which is used for the first time in Al - Najaf Al-Ashraf governorate) in screening for pulmonary tuberculosis as a rapid diagnostic test of pulmonary tuberculosis, in comparison with sputum smear microscopy depending on the (findings and clinical judgment). Patients involved were those who attend at Respiratory Chest Diseases Center in AL– Najaf ALAshraf Governorate. Fifty suspected patients, within the age groups 12 - 70 years …

In Vitro Study For The Effect Of Cinnamon Oil On Multi-Drug Resistant Staphylococcus Aureus And Proteus Mirabilis Strains, Israa Kitab Al -Yasiri

Al-Qadisiyah Medical Journal

The study was carried out to assess the effect of cinnamon oil on multi-drug resistant (MDR) strains of Staphylococcus aureus and Proteus mirabilis isolated from patient infected with otitis media. 50 specimens were collected at AL Hakeem Teaching Hospital in AL-Najaf governorate, 20 isolates were found to be Staphylococcus spp. and Proteus spp. on further studying for the species level only six of these isolates were found to be multi-drug resistant Staphylococcus aureus and Proteus mirabilis. The results of antibiotic sensitivity testing showed that most of these isolates were highly resistant (resistance percentage was 40-90%) for the used antibiotics. The …

The Frequency Of Ophthalmia Neonatorum In Hospitalized Neonates And Newborn Babies Delivered In The Maternity And Children Teaching Hospital In Diwaniah, Mohammed Mojar Al-Shamsi, Adnan Hamad Al-Hamdani, Najlaa Abdullah Dawood

Al-Qadisiyah Medical Journal

Objectives:Determine the frequency of ophthalmia neonatorum among hospitalized neonates and those delivered in the labor room and operation theatres in the maternity and children teaching hospitals in Diwaniah in addition to determination of the causative microorganisms and their antibiotic sensitivity.Materials & methods:Two hundreds seventy conjunctival swabs were collected randomly from neonates admitted to the septic and aseptic neonatal care units and newborn babies delivered in the labor room and operation theatres in the maternity and children teaching hospitalDiwaniah during the period from September 2002 to February 2003.Results : The clinical and microbiological diagnosis of ophthalmia neonatorum was made in 190 …

The Risk Of Infertility In The Chlamydial Vaginal Infection, Manal Mohammed Kadhim Al-Saadi

Al-Qadisiyah Medical Journal

Across-section study carried on 100 women attended the Maternity and Child Teaching Hospital and certain public clinics in Diwanyia city from the first of march 2005 to the end of August 2005 ,and complete medical history was taken from each one ,then a serological test was done by using Chlamydial Kit (Biorapid Chlamydia antigen)to identify the rate of infection with Chlamydia and its correlation with risk of infertility in women .The study was revealed that the prevalence rate of chlamydial infection was 26% among women ,and there is an risk factor or Odds ratio (OR) =6 to produce infertility among …

Relationship Between Ada Level And Type Of Bacterial Causative Agents Of Chronic Suppurative Otitis Media, Habbeb S.Naher

Al-Qadisiyah Medical Journal

A total of 344 ear swabs were obtained from 300 outpatients. ( 152 were female and 148 were male ) .256 of them had unilateral and 44 had bilateral otorrhea , attending to the ENT-Department at general teaching hospital in Najaf governorate for the period from 1st December 1999 to of July 2000 .all patients submitted to ENT-examination by physicians .in addition to that 3ml of venous blood were aspirated from 41 patients with this disease and 45 normal healthy individuals carefully selected as a control for assessment the adenosine deaminase (ADA) enzyme specific activity . Pseudomonas aeruginosa ( the …

An Examination Of The Effect On Health Protective Behaviour Of Diabetes And Obesity Awareness And Personal Characteristics In Adults: Structural Equation Modeling, Ayşe Soylu, Pinar Şahin

Saudi Medical Journal

Objectives: The World Health Organization has reported that obesity-related diabetes cases are increasing rapidly. The aim of this study was to examine the effect on health protective behaviour of diabetes and obesity awareness and personal characteristics in adults. Method: This descriptive, cross-sectional, correlational study was conducted with 1241 participants. Data were collected using a Personal Information Form, the Diabetes and Obesity Awareness Scale and Health Protective Behaviour Scale. Data analysis was performed with IBM SPSS vn.23 and IBM AMOS vn.24 software. STROBE was used in reporting. Results: Of the total participants, 45.4% had no diabete and obesity awareness and in …

Existence Of Positive Periodic Solutions For A First-Order Semilinear Functional Differential Equation With An Iterative Source Term, Ahlème Bouakkaz, Rabah Khemis

Turkish Journal of Mathematics

In this paper, we will establish the existence of positive periodic solutions for a class of first order iterative functional differential equations by using a powerful and effective technique based on the Krasnoselskii fixed point theorem together with some useful properties of a Green's function. The obtained results that complement some previous works, are illustrated with an example.

Assessment Of Antimicrobial Transition Errors From Hospitals To Skilled Nursing Facilities, Amy Y. Kang, Guarina A. Garcia Delgado, Ashley Nguyen, Maddie Yeh, Tracy Ly, Richard C. Beuttler, Abisay Ortega, Donna Phan Tran, Evelyn Flores, Charis Tjeong, Andrew Bishop, Praneet Kalkat, Loren G. Miller

Pharmacy Faculty Articles and Research

Background More than 1 million Americans reside in skilled nursing facilities (SNFs). Antimicrobial transition errors among patients transferred from hospital to SNFs pose safety risks and may lead to poor outcomes, but data on such errors are limited.   Methods We conducted a retrospective cohort study of infectious diseases clinics from 1 June 2020 through 30 November 2023 at the Los Angeles County Department of Health Services, a large safety-net health system. We performed logistic regression analyses to identify factors associated with antimicrobial transition errors and poor infection outcomes.   Results We screened records of 6865 clinic patients, among whom 112 were …

Bacterial Fecal Microbiome Composition Associated With Hiv Stage Among Rural And Peri-Urban Adults Living With Hiv In Uganda, Bożena M Morawski, Elise R Morton, Miya Yunus, Claudia Muñoz-Zanzi, Rojelio Mejia, Ran Blekhman, David R Boulware, Sarah M Lofgren

Faculty, Staff and Students Publications

Background

Gut microbiome composition in HIV-infection provides insight into HIV pathogenesis and potentially offers avenues to adjunctive HIV therapies. Although two-thirds of people living with HIV (PLHIV) are in Africa, studies of the gut microbiome PLHIV have focused on non-African populations. Additionally, social, cultural, and environmental factors influence the gut microbiome and differ substantially between African and Western and urban and rural communities. Here, we quantify the relationships between immune status and fecal microbiome composition in 175 rural Ugandan PLHIV.

Methods

In 2013, 175 Ugandan PLHIV contributed a single fecal sample as part of a cohort study of 202 participants. …