Digital Commons Network^™

Ethanol And Anaerobic Conditions Reversibly Inhibit Commercial Cellulase Activity In Thermophilic Simultaneous Saccharification And Fermentation (Tssf), Kara K. Podkaminer, William R. Kenealy, Christopher D. Herring, David A. Hogsett, Lee R. Lynd

Dartmouth Scholarship

A previously developed mathematical model of low solids thermophilic simultaneous saccharification and fermentation (tSSF) with Avicel was unable to predict performance at high solids using a commercial cellulase preparation (Spezyme CP) and the high ethanol yield Thermoanaerobacterium saccharolyticum strain ALK2. The observed hydrolysis proceeded more slowly than predicted at solids concentrations greater than 50 g/L Avicel. Factors responsible for this inaccuracy were investigated in this study.

Clay Minerals For Nanocomposites And Biotechnology: Surface Modification, Dynamics And Responses To Stimuli, Hendrik Heinz

College of Polymer Science and Polymer Engineering

Clay minerals find a wide range of application in composites, paints, drilling liquids, cosmetics, and medicine. This article reviews chemical and physical properties of natural and organically modified clay minerals to understand the nanometre-scale structure, surface characteristics, and application in functional materials. The relation between fundamental properties and materials design is emphasized and illustrated by examples. The discussion comprises the following: an overview; surface structure and cation density; solubility and solubility reversal by surface modification; the degree of covalent and ionic bonding represented by atomic charges; the distribution of metal substitution sites; measurements and simulations of interfacial properties at the …

A Novel Ascaroside Controls The Parasitic Life Cycle Of The Entomopathogenic Nematode Heterorhabditis Bacteriophora, Jaime H. Noguez, Elizabeth S. Conner, Yue Zhou, Todd A. Ciche, Justin R. Ragains, Rebecca A. Butcher

Faculty Publications

Entomopathogenic nematodes survive in the soil as stress-resistant infective juveniles that seek out and infect insect hosts. Upon sensing internal host cues, the infective juveniles regurgitate bacterial pathogens from their gut that ultimately kill the host. Inside the host, the nematode develops into a reproductive adult and multiplies until unknown cues trigger the accumulation of infective juveniles. Here, we show that the entomopathogenic nematode Heterorhabditis bacteriophora uses a small-molecule pheromone to control infective juvenile development. The pheromone is structurally related to the dauer pheromone ascarosides that the free-living nematode Caenorhabditis elegans uses to control its development. However, none of the …

On The Automatic Recognition Of Human Activities Using Heterogeneous Wearable Sensors, Oscar David Lara Yejas

USF Tampa Graduate Theses and Dissertations

Delivering accurate and opportune information on people's activities and behaviors has become one of the most important tasks within pervasive computing. Its wide spectrum of potential applications in medical, entertainment, and tactical scenarios, motivates further

research and development of new strategies to improve accuracy, pervasiveness, and eciency.

This dissertation addresses the recognition of human activities (HAR) with wearable sensors in three main regards: In the rst place, physiological signals have been incorporated as a new source of information to improve the recognition accuracy achieved by conventional approaches, which rely on accelerometer signals solely. A new HAR system, Centinela, was born …

Focal Adhesion Kinase Modulates Cdc42 Activity Downstream Of Positive And Negative Axon Guidance Cues, Jonathan P. Myers, Estuardo Robles, Allison Ducharme-Smith, Timothy M. Gomez

Neuroscience: Faculty Publications

There is biochemical, imaging and functional evidence that Rho GTPase signaling is a crucial regulator of actin-based structures such as lamellipodia and filopodia. However, although Rho GTPases are believed to serve similar functions in growth cones, the spatiotemporal dynamics of Rho GTPase signaling has not been examined in living growth cones in response to known axon guidance cues. Here we provide the first measurements of Cdc42 activity in living growth cones acutely stimulated with both growth-promoting and growthinhibiting axon-guidance cues. Interestingly, we find that both permissive and repulsive factors can work by modulating Cdc42 activity, but in opposite directions. We …

Now It Is Impossible 'Simply To Continue Along Previous Lines': A Partial Design Sketch Of Enactable Social Theorizing, Peter J. Taylor

Working Papers on Science in a Changing World

A compilation of 39 notes provides the basis for two shifts: from shaping a better social theory to allowing for social theorizing; and from representing social dynamics to enacting the social theorizing so as to repeatedly define and pursue engagements in the heterogeneous dynamics that intersect in all kinds of society-making. A key move is to bring the multiple strandedness of changing social life into the center by combining, on one hand, the analysis of intersecting processes, which link across scales in the production of any outcome and in their own on-going transformation, and, on the other hand, a participatory …

Notes On Distribution And Hosts Of Hylesinus Mexicanus (Wood) (Coleoptera: Curculionidae: Scolytinae), A Pest On Olea Europaea Linnaeus, Jesus Romero-Napoles, Thomas H. Atkinson

Insecta Mundi

Significant new host and distribution records are presented for Hylesinus mexicanus (Wood) (Coleoptera: Curculionidae: Scolytinae), including its pest potential on cultivated olives. Hylesinus mexicanus is similar to H. fasciatus LeConte and information presented here suggests that the distinctiveness of these two species needs to be re-examined.

Observations Of Plume Moths On North Andros Island, Bahamas, And Notes On New Records And Species Previously Recorded From The Bahamas (Lepidoptera: Pterophoridae), Deborah L. Matthews, Jacqueline Y. Miller, Mark J. Simon, Gary Goss

Insecta Mundi

Recent fieldwork on North Andros Island by the authors resulted in the collection of six species of Pterophoridae (Lepidoptera), five of which were previously unrecorded for the Bahamas in published accounts. Three additional species are noted for the Bahamian fauna based on specimens collected in the 1980s on other islands. Representative specimens are illustrated from North Andros along with genitalic images for species where these are not readily available in other publications. In addition, images of the larva and pupa are provided for a reared species for which the life history was previously unknown.

Argentina Passes Far-Reaching Gender-Identity, Death-With-Dignity Laws, Andrã©S Gaudã­N

NotiSur

No abstract provided.

Lapg, Required For Modulating Biofilm Formation By Pseudomonas Fluorescens Pf0-1, Is A Calcium-Dependent Protease, Chelsea D. Boyd, Debashree Chatterjee, Holger Sondermann, George A. O'Toole

Dartmouth Scholarship

Biofilm formation by Pseudomonas fluorescens Pf0-1 requires the cell surface adhesin LapA. We previously reported that LapG, a periplasmic cysteine protease of P. fluorescens, cleaves the N terminus of LapA, thus releasing this adhesin from the cell surface and resulting in loss of the ability to make a biofilm. The activity of LapG is regulated by the inner membrane-localized cyclic-di-GMP receptor LapD via direct protein-protein interactions. Here we present chelation and metal add-back studies demonstrating that calcium availability regulates biofilm formation by P. fluorescens Pf0-1. The determination that LapG is a calcium-dependent protease, based on in vivo and in vitro …

Probing Structural Selectivity Of Synthetic Heparin Binding To Stabilin Protein Receptors, Elizabeth H. Pempe, Yongmei Xu, Sandhya Gopalakrishnan, Jian Liu, Edward N. Harris

Department of Biochemistry: Faculty Publications

As one of the most widely used drugs worldwide, heparin is an essential anticoagulant required for surgery, dialysis, treatment of thrombosis, cancer, and general circulatory management. Stabilin- 2 is a scavenger clearance receptor with high expression in the sinusoidal endothelium of liver. It is believed that Stabilin- 2 is the primary receptor for the clearance of unfractionated and low molecular weight heparins in the liver. Here, we identify the modifications and length of the heparin polymer that are required for binding and endocytosis by both human Stabilin receptors: Stabilin-2 and its homolog Stabilin-1 (also found in liver endothelium). Using enzymatically …

Engrailed Cooperates Directly With Extradenticle And Homothorax On A Distinct Class Of Homeodomain Binding Sites To Repress Sloppy Paired., Miki Fujioka, Brian Gebelein, Zenobia C Cofer, Richard S Mann, James B Jaynes

Department of Biochemistry and Molecular Biology Faculty Papers

Even skipped (Eve) and Engrailed (En) are homeodomain-containing transcriptional repressors with similar DNA binding specificities that are sequentially expressed in Drosophila embryos. The sloppy-paired (slp) locus is a target of repression by both Eve and En. At blastoderm, Eve is expressed in 7 stripes that restrict the posterior border of slp stripes, allowing engrailed (en) gene expression to be initiated in odd-numbered parasegments. En, in turn, prevents expansion of slp stripes after Eve is turned off. Prior studies showed that the two tandem slp transcription units are regulated by cis-regulatory modules (CRMs) with activities that overlap in space and time. …

Classification Of Phase Transition Behavior In A Model Of Evolutionary Dynamics, Dawn Michelle King

Theses

Amongst the scientific community, there is consensus that evolution has occurred; however, there is much disagreement about how evolution happens. In particular, how do we explain biodiversity and the speciation process? Computational models aid in this study, for they allow us to observe a speciation process within time scales we would not otherwise be able to observe in our lifetime. Previous work has shown phase transition behavior in an assortative mating model as the control parameter of maximum mutation size (µ) is varied. This behavior has been shown to exist on landscapes with variable fitness (Dees and Bahar, 2010), and …

06-14-2012 Two High School Grads Benefit From Research Opportunities Provided By Nsf Grant To Swosu, Southwestern Oklahoma State University

THE BARK ARCHIVE 2012

Southwestern Oklahoma State University and Dr. Andrea Holgado recently received a $10,500 grant from the National Science Foundation for research experiences for two high school graduates.

Macalester Receives Grant From The Canadian Institutes Of Health Research To Study Aging, Macalester College

College Press Releases

No abstract provided.

Composition Of The Adult Digestive Tract Bacterial Microbiome Based On Seven Mouth Surfaces, Tonsils, Throat And Stool Samples, Nicola Segata, Susan Kinder Haake, Peter Mannon, Katherine P. Lemon, Levi Waldron, Dirk Gevers, Curtis Huttenhower, Jacques Izard

Publications and Research

Background: To understand the relationship between our bacterial microbiome and health, it is essential to define the microbiome in the absence of disease. The digestive tract includes diverse habitats and hosts the human body’s greatest bacterial density. We describe the bacterial community composition of ten digestive tract sites from more than 200 normal adults enrolled in the Human Microbiome Project, and metagenomically determined metabolic potentials of four representative sites.

Results: The microbiota of these diverse habitats formed four groups based on similar community compositions: buccal mucosa, keratinized gingiva, hard palate; saliva, tongue, tonsils, throat; sub- and supra-gingival plaques; and stool. …

The Body Plan Concept And Its Centrality In Evo-Devo, Katherine E. Willmore

Paediatrics Publications

A body plan is a suite of characters shared by a group of phylogenetically related animals at some point during their development. The concept of bauplane, or body plans, has played and continues to play a central role in the study of evolutionary developmental biology (evo-devo). Despite the importance of the body plan concept in evo-devo, many researchers may not be familiar with the progression of ideas that have led to our current understanding of body plans, and/or current research on the origin and maintenance of body plans. This lack of familiarity, as well as former ties between the body …

Weighing In On Teacher Attitudes Of Obese Students: A Descriptive Correlational Design, Angelina Pecoraro

Theses and Dissertations

The primary purpose of this study was to investigate teacher attitudes towards obese students. The investigation focused on the correlation between (a) attitudes and beliefs about obese students; and (b) predictive abilities of participant's self-esteem with attitudes and beliefs. The three surveys utilized in this study were: (a) Attitudes Toward Obese Persons Scale (Allison, Basile, & Yuker, 1991) (b) Beliefs About Obese Persons (Allison et al., 1991); and (c) Rosenberg Self-Esteem Survey (Rosenberg, 1965). The sample size was 893 certified educators representing all grade levels in Central, Northern, and Southern New Jersey school districts. Respondents were emailed a survey link, …

The Putative Cannabinoid Receptor Gpr55 Modulates Synaptic Plasticity In The Hippocampus, Corinne Marie Badgley

Theses and Dissertations

Endocannabinoids (eCBs) are small molecules that are capable of modulating synaptic plasticity of both excitatory and inhibitory synapses in the brain. While eCBs bind to transient receptor potential vanilloid 1 (TRPV1) and cannabinoid receptor 1 (CB1) in the central nervous system, we recently identified a form of non-CB1, non-TRPV1 mediated long term depression activated by the eCB anandamide at CA1 hippocampal stratum radiatum interneurons. GPR55, an orphan G-protein receptor, has been identified in the hippocampus and is capable of activation by eCBs, making it a good candidate for mediating this non-CB1, non-TRPV1 form of synaptic plasticity. Here we performed whole-cell …

Adult Vampire Bats Produce Contact Calls When Isolated: Acoustic Variation By Species, Population, Colony, And Individual, Gerald G. Carter, Ryane Logsdon, Bryan D. Arnold, Angelica Menchaca, Rodrigo A. Medellin

KIP Articles

Bat pups produce individually distinct isolation calls to facilitate maternal recognition. Increasing evidence suggests that, in group-living bat species, adults often use similar calls to maintain contact. We investigated if isolated adults from all three species of the highly cooperative vampire bats (Phyllostomidae: Desmodontinae) would produce vocally distinct contact calls when physically isolated.

Process Flow Features As A Host-Based Event Knowledge Representation, Benhur E. Pacer

Theses and Dissertations

The detection of malware is of great importance but even non-malicious software can be used for malicious purposes. Monitoring processes and their associated information can characterize normal behavior and help identify malicious processes or malicious use of normal process by measuring deviations from the learned baseline. This exploratory research describes a novel host feature generation process that calculates statistics of an executing process during a window of time called a process flow. Process flows are calculated from key process data structures extracted from computer memory using virtual machine introspection. Each flow cluster generated using k-means of the flow features represents …

Further Evidence For The Likely Completeness Of The Library Of Solved Single Domain Protein Structures, Jeffrey Skolnick, Hongyi Zhou, Michal Brylinski

Faculty Publications

Recent studies questioned whether the Protein Data Bank (PDB) contains all compact, single domain protein structures. Here, we show that all quasi-spherical, QS, random protein structures devoid of secondary structure are in the PDB and are excellent templates for all native PDB proteins up to 250 residues. Because QS templates have a similar global contour as native, TASSER can refine 98% (90%) of those whose TM-score is 0.4 (0.35) to structures greater than or equal to the 0.5 TM-score threshold (0.74 (0.64) mean TM-score) for CATH/SCOP assignment. On the basis of this and the fact that, at a TM-score of …

Silica Supported Silver Nanoparticles Catalyst Systems For Oxidation Of Dbt In Crude Oil., Ashlie Santaliz, Brenda Torres, Maryam Zarei, Manuel Ramos, Russel R. Chianelli

COURI Symposium Abstracts, Summer 2012

Metal nanoparticles (NP’s) have become a subject of intense interest in various fields of chemistry and physics primarily because of its catalytic activity at their specific surfaces. Their catalytic activity depending solely of their factes, it is recomendable to achieve six-fold symmetry along with edge sites, ideal places for the attachment of molecules. The aim of this project is to achieve the synthesis of NP-Ag and their functionalizatiin using supported on silica to later perform an oxidation model reaction of diobenzothiophene (DBT) to diobenzenethiophene-sulfone. The DBT oxidation reaction was performed in a reflux system using DBT and silica solved in …

Human Breast Milk And Antiretrovirals Dramatically Reduce Oral Hiv-1 Transmission In Blt Humanized Mice, Angela Wahl, Michael D. Swanson, Tomonori Nochi, Rikke Olesen, Paul W. Denton, Morgan Chateau, J. Victor Garcia

Biology Faculty Publications

Currently, over 15% of new HIV infections occur in children. Breastfeeding is a major contributor to HIV infections in infants. This represents a major paradox in the field because in vitro, breast milk has been shown to have a strong inhibitory effect on HIV infectivity. However, this inhibitory effect has never been demonstrated in vivo. Here, we address this important paradox using the first humanized mouse model of oral HIV transmission. We established that reconstitution of the oral cavity and upper gastrointestinal (GI) tract of humanized bone marrow/liver/thymus (BLT) mice with human leukocytes, including the human cell types important for …

Trends In Mortality From Septicaemia And Pneumonia With Economic Development: An Age-Period-Cohort Analysis, Irene O.L. Wong, Benjamin J. Cowling, Gabriel M. Leung, Mary Schooling

Publications and Research

Background
Hong Kong population has experienced drastic changes in its economic development in the 1940s. Taking advantage of Hong Kong’s unique demographic and socioeconomic history, characterized by massive, punctuated migration waves from Southern China, and recent, rapid transition from a pre-industrialized society to the first ethnic Chinese community reaching ‘‘first world’’ status over the last 60 years (i.e., in two or three generations), we examined the longitudinal trends in infection related mortality including septicemia compared to trends in non-bacterial pneumonia to generate hypotheses for further testing in other recently transitioned economies and to provide generalized aetiological insights on how economic …

Microbial Communities Shifted Dramatically After Deepwater Horizon Spill, Unh Communications And Public Affairs

UNH Today Archive

No abstract provided.

Persistence And Foliation Theory And Their Application To Geometric Singular Perturbation, Ji Li

Theses and Dissertations

Persistence problem of compact invariant manifold under random perturbation is considered in this dissertation. Under uniformly small random perturbation and the condition of normal hyperbolicity, the original invariant manifold persists and becomes a random invariant manifold. The random counterpart has random local stable and unstable manifolds. They could be invariantly foliated thanks to the normal hyperbolicity. Those underlie an extension of the geometric singular perturbation theory to the random case which means the slow manifold persists and becomes a random manifold so that the local global structure near the slow manifold persists under singular perturbation. A normal form for a …

Structure, Function And Diversity Of The Healthy Human Microbiome, Curtis Huttenhower, Jacques Izard

Department of Food Science and Technology: Faculty Publications

Studies of the human microbiome have revealed that even healthy individuals differ remarkably in the microbes that occupy habitats such as the gut, skin and vagina. Much of this diversity remains unexplained, although diet, environment, host genetics and early microbial exposure have all been implicated. Accordingly, to characterize the ecology of human-associated microbial communities, the Human Microbiome Project has analysed the largest cohort and set of distinct, clinically relevant body habitats so far. We found the diversity and abundance of each habitat’s signature microbes to vary widely even among healthy subjects, with strong niche specialization both within and among individuals. …

A Framework For Human Microbiome Research, Barbara A. Methe, Jacques Izard

Department of Food Science and Technology: Faculty Publications

A variety of microbial communities and their genes (the microbiome) exist throughout the human body, with fundamental roles in human health and disease. The National Institutes of Health (NIH)-funded Human Microbiome Project Consortium has established a population-scale framework to develop metagenomic protocols, resulting in a broad range of quality-controlled resources and data including standardized methods for creating, processing and interpreting distinct types of high-throughput metagenomic data available to the scientific community. Here we present resources from a population of 242 healthy adults sampled at 15 or 18 body sites up to three times, which have generated 5,177 microbial taxonomic profiles …

Cyberspace And Real-World Behavioral Relationships: Towards The Application Of Internet Search Queries To Identify Individuals At-Risk For Suicide, Casey C. Miller

Theses and Dissertations

The Internet has become an integral and pervasive aspect of society. Not surprisingly, the growth of ecommerce has led to focused research on identifying relationships between user behavior in cyberspace and the real world - retailers are tracking items customers are viewing and purchasing in order to recommend additional products and to better direct advertising. As the relationship between online search patterns and real-world behavior becomes more understood, the practice is likely to expand to other applications. Indeed, Google Flu Trends has implemented an algorithm that accurately charts the relationship between the number of people searching for flu-related topics on …