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2012 Work Plan Usf Sarasota-Manatee, Usf

USF Accountability Reports

No abstract provided.

2012 Work Plan Usf System, Usf

USF Accountability Reports

No abstract provided.

2012 Annual Report Usf Sarasota-Manatee, Usf

USF Accountability Reports

No abstract provided.

2012 Work Plan Usf St. Petersburg, Usf

USF Accountability Reports

No abstract provided.

Bio-Logic Builder: A Non-Technical Tool For Building Dynamical, Qualitative Models, Tomáš Helikar, Bryan Kowal, Alex Madrahimov, Manish Shrestha, Jay Pedersen, Kahani Limbu, Ishwor Thapa, Thaine Rowley, Rahul Satalkar, Naomi Kochi, John Konvalina, Jim A. Rogers

Department of Biochemistry: Faculty Publications

Computational modeling of biological processes is a promising tool in biomedical research. While a large part of its potential lies in the ability to integrate it with laboratory research, modeling currently generally requires a high degree of training in mathematics and/or computer science. To help address this issue, we have developed a web-based tool, Bio- Logic Builder, that enables laboratory scientists to define mathematical representations (based on a discrete formalism) of biological regulatory mechanisms in a modular and non-technical fashion. As part of the user interface, generalized ‘‘biologic’’ modules have been defined to provide users with the building blocks for …

Experimental Simulation Of Human Islet Amyloid Polypeptide (Hiapp)-Pancreatic Beta Cell Membrane Interactions: Inferences And Implications In The Etiopathogenesis Of Diabetes Mellitus Type Ii, Sarika Pamarthy

Master's Theses and Doctoral Dissertations

Pancreatic beta cells secrete insulin, an endocrine hormone that regulates blood glucose levels and maintains normal physiological activity in humans and animals. Diabetes mellitus type II is a consequence of the gradual destruction of these important cells, likely by human islet amyloid polypeptide (hIAPP) that is co-secreted with insulin. Increasing health care costs, coupled with the World Health Organization’s prediction of a worldwide diabetic epidemic by year 2030, make experimental diabetes research a crucial prologue to future clinical trials in prevention, diagnosis, and treatment of Diabetes mellitus type II. Our experimental set-up simulates hIAPP peptide fragment and pancreatic beta cell …

Speech Intelligibility And Prosody Production In Children With Cochlear Implants, Steven B. Chin, Tonya R. Bergeson, Jennifer Phan

Scholarship and Professional Work - Communication

Objectives—The purpose of the current study was to examine the relation between speech intelligibility and prosody production in children who use cochlear implants. Methods—The Beginner's Intelligibility Test (BIT) and Prosodic Utterance Production (PUP) task were administered to 15 children who use cochlear implants and 10 children with normal hearing. Adult listeners with normal hearing judged the intelligibility of the words in the BIT sentences, identified the PUP sentences as one of four grammatical or emotional moods (i.e., declarative, interrogative, happy, or sad), and rated the PUP sentences according to how well they thought the child conveyed the designated mood. Results—Percent …

Application Of Default Rules To Address Financial Conflicts Of Interest In Academic Medical Centers, Joanna K. Sax

Indiana Law Journal

This Essay proposes that the rules governing financial conflicts of interest for scientists within the National Institutes of Health apply to scientists at Academic Medical Centers because scientists at both places receive federal funding. The rules governing financial conflicts of interest within the National Institutes of Health are stricter than the rules at Academic Medical Centers. The cornerstone of financial conflicts of interest rules at Academic Medical Centers is disclosure, which is inadequate. This Essay builds on previous work calling for significant changes to rules addressing financial conflicts of interest, and it promotes changes by calling for the application of …

Cationic Amphiphilic Drug-Induced Autophagosome Accumulation Is Due To Autophagosome Sequestration Within Vimentin Intermediate Filament Networks Resulting In Prolonged Autophagosome Half-Life, Miriam Devorah Kleinman

Wayne State University Dissertations

Accumulations of autophagosomes and non-esterified cholesterol are

observed in several cell lines derived from lysosomal storage diseases,

including Niemann Pick Type C (NPC). The relationship between

autophagosome accumulation and lysosomal non-esterified cholesterol is

unclear. Exposure of murine hepatoma 1c1c7 cultures to the cationic

amphiphilic drugs (CADs) U18666A, imipramine and clozapine caused

lysosomal non-esterified cholesterol and autophagosome accumulation.

Measurement of LC3-II conversion in the presence of lysosomal inhibitors

bafilomycin A1 and NH4Cl, degradation of long-lived proteins, and

colocalization of GFP-LC3 and LAMP1 indicated an increase in

autophagosome synthesis without compensatory increase in clearance.

Autophagosome synthesis was blocked using 3-MA to monitor …

Patents Fettering Reproductive Rights, Scott A. Allen

Indiana Law Journal

No abstract provided.

A Warm Watchful Eye: Parental Emotional Support And Monitoring In Multisystem Therapy For Youth With Diabetes, Dana K. May

Wayne State University Theses

The purpose of this study was to examine the extent to which changes in parenting behaviors, in particular monitoring diabetes care completion and providing emotional support to the youth, improve health care status within participants in a Multisystemic psychotherapeutic intervention (MST). RM-ANOVAs were used to determine the relationship of parental involvement in the treatment and telephone support group over time. Change score correlations were used to examine changes in parental involvement relationship to changes in diabetes care behaviors. All participants in the study increased in parental monitoring over time, with no differences between the MST group and the telephone support …

Mrna Granules And Ischemic Preconditioning, Michelle Rei Smith

Wayne State University Theses

Brain ischemia and reperfusion that occurs after stroke and cardiac arrest, causes translation arrest (TA) in neurons which is irreversible in neurons that will undergo delayed neuronal death. TA is linked to mRNA granules, which are involved in ischemia-induced stress genes translation. Ischemic preconditioning (IPC) is the most protective response known that protects neurons from a lethal ischemic insult. In this thesis I studied the effects of (1) sublethal durations of ischemia, (2) IPC and (3) cycloheximide (CHX) on the formation of mRNA granules at 1 hour of reperfusion and the colocalization of HuR in the mRNA granules. All durations …

Timing Of Residential Electric Loads To Reduce Air Emissions From Power Generation, Michelle Marinich Rogers

Wayne State University Theses

Over the course of a day, power utilities must respond to changing demand by dispatching or shedding output from different generators. Each generator is associated with a unique profile of air emissions, based on the type of fuel consumed, installed pollution controls, and the generator's efficiency. The aim of this study is to determine whether shifting electric demand to times when cleaner generation sources are available would result in overall emissions reduction.

Dynamic wholesale pricing in the electric power market, specifically the Locational Marginal Price (LMP), provides a means to estimate the marginal generator fuel type. This knowledge was used …

The Role Of Uracil-Dna Glycosylase And Folate In The Repair Of Dna, Sarah Talal Dubaisi

Wayne State University Theses

Abstract

Role of Uracil DNA-Glycosylase and Folate

In the Repair of Oxidative Damage

By

Sarah Dubaisi

May2012

Advisor: Dr. Diane Cabelof

Major: Nutrition and Food Science

Degree: Masters of Science

The impact of DNA damage on genomic integrity has been widely investigated due to its association with cancer and ageing. DNA repair pathways play a critical role in preserving the genomic integrity and protecting the cells against DNA damage caused by oxidative stress and folate deficiency. Base Excision Repair (BER) is one of the major pathways involved in the cellular response to DNA damage, primarily the damage caused by oxidative …

Modes And Mechanisms Of Hfq Mediated Stress Regulation In Bacteria, Nilshad Nilam Salim

Wayne State University Dissertations

To survive bacteria must be able to respond to its ever-changing environmental conditions. sRNAs have been implicated in a variety of stress-response pathways that help bacterial systems modulate gene expression. The RNA binding protein Hfq facilities this process by, helping sRNA to base pair with its target mRNAs to initiate gene regulation. A common feature of Hfq-mediated gene regulation is the network-based organization where a single sRNA can control multiple messages to promote integrated response to stress. Current mechanistic models that are present to describe Hfq functions cannot explain the complexity at which Hfq performs gene regulation. In this work …

Drug Resistance Mechanisms And Drug Design Strategies For Human Immunodeficiency Virus And Hepatitis C Virus Proteases, Yong Wang

Wayne State University Dissertations

The antiviral drug development has improved steadily to treat the infections of human immunodeficiency virus (HIV) and hepatitis C virus (HCV) which represent heavy public health burdens. The viral protease plays an indispensable role in viral maturation and therefore becomes one of the most important targets for drug design. Nine HIV-1 protease inhibitors and two HCV protease inhibitors have been developed and approved by the U.S. Food and Drug Administration. However, mutations in the protease decrease reduce the efficacy the drugs. In this study, the enzyme assays indicate that darunavir and tipranavir exhibit the most potent inhibition against the multi-drug …

Single Molecule Studies Of Rna-Target Interactions, Sharla Leann Wood

Wayne State University Dissertations

We have used FRET, single molecule spectroscopy, and several other biophysical techniques to study how the folding of RNA allows it to perform its various functions by recognizing and binding a target ligand. We have shown that the c-di-GMP riboswitch undergoes a large, global conformational change upon binding of the ligand. The folding dynamics of the c-di-GMP riboswitch upon Mg2+ binding help to pre-organize the aptamer for efficient ligand binding and ultimately efficient gene expression. We have also investigated two instances, a fluorophore-binding aptamer and molecular beacon, where the folding of RNA can be used to detect an analyte. We …

Characterization Of Intracellular Interactions Between Dengue Virus And Host Proteins, Dumrong Mairiang

Wayne State University Dissertations

Dengue virus is the causative agent of dengue fever, dengue hemorrhagic fever and dengue shock syndrome. About two-fifths of world population live in areas where dengue is prevalent, leading to high levels of morbidity and mortality in many areas. Currently there are no vaccines or effective treatments. The virus is transmitted from one person to another by the yellow fever mosquito, Aedes aegypti. The genome of dengue virus encodes only ten proteins implying that the virus needs to interact with and utilize several host proteins for replication. In this project, I used high-throughput yeast two-hybrid screening to identify mosquito and …

Synthesis Of Oligosaccharide Mimetics By The Desulfurative Rearrangement Of Allylic Disulfides And Stereoelectronic Influence Of C-O Bonds On C- And O-Glycosylation, Myriame Moume-Pymbock

Wayne State University Dissertations

This dissertation focuses on two different problems. The first part of this manuscript presents the application of a novel methodology to access the synthesis of oligosaccharide mimetics while the second part focuses on the study of the stereoelectronic effects of proximal C-O bonds in C- and O-glycosylation reactions.

The first chapter provides a brief overview of the important roles that oligosaccharides and glycoconjugates play in biological processes and sets out the major advances that have been made in the past few decades in the synthesis of such complex structures while bringing out the ongoing challenges and the limitations that impede …

Hyperosmotic Stress Enzyme Signaling Modulates Oct4, Nanog, And Rex1 Expression And Induces Prioritized Differentiation Of Murine Embryonic Stem Cells, Jill A. Slater

Wayne State University Dissertations

HYPEROSMOTIC STRESS ENZYME SIGNALING MODULATES OCT4, NANOG,

AND REX1 EXPRESSION AND INDUCES PRIORITIZED DIFFERENTIATION OF

MURINE EMBRYONIC STEM CELLS

by

JILL SLATER

MAY 2013

Advisor: Daniel Rappolee, Ph.D.

Major: Physiology

Degree: Doctor of Philosophy

Transcription factor expression and therefore lineage identity in the periimplantation

embryo and its stem cells may be influenced by extracellular stresses,

potentially affecting pregnancy outcome. Cellular stress forces cells to suppress some

normal activities (such as protein synthesis and cell proliferation) in order to repair

stress-damaged macromolecules and restore homeostasis. Therefore, any new

activities that embryonic cells initiate while concurrently funding the demands of the

stress …

Correlation Analyses Of Clinical And Molecular Findings Identify Candidate Biological Pathways In Systemic Juvenile Idiopathic Arthritis, Xuefeng B. Ling, Claudia Macaubas, Heather C. Alexander, Qiaojun Wen, Edward Chen, Sihua Peng, Yue Sun, Chetan Deshpande, Kuang-Hung Pan, Richard Lin, Chih-Jian Lih, Sheng-Yung P. Chang, Tzielan Lee, Christy Sandborg, Ann B. Begovich, Stanley N. Cohen, Elizabeth D. Mellins

Wayne State University Associated BioMed Central Scholarship

Abstract

Background

Clinicians have long appreciated the distinct phenotype of systemic juvenile idiopathic arthritis (SJIA) compared to polyarticular juvenile idiopathic arthritis (POLY). We hypothesized that gene expression profiles of peripheral blood mononuclear cells (PBMC) from children with each disease would reveal distinct biological pathways when analyzed for significant associations with elevations in two markers of JIA activity, erythrocyte sedimentation rate (ESR) and number of affected joints (joint count, JC).

Methods

PBMC RNA from SJIA and POLY patients was profiled by kinetic PCR to analyze expression of 181 genes, selected for relevance to immune response pathways. Pearson correlation and Student's t-test …

Hyphenated Artists: A Body Of Potential, Laura Reeder

Journal of Social Theory in Art Education

The author of this article, an art teacher, arts education advocate, teaching artist, pre-service art teacher supervisor and instructor confronts “either/or” professional identities in arts education. Multi-faceted artist/scholar/educator/ learner/advocate/personas are “unfenced” in order to navigate spaces of artistic, educational, and cultural production without having to pause for identification at borders. In this form, pedagogies for inventive social change emerge. Dialogue among fields of artists and educators links either/or, artist/teacher qualities in holistic and interdisciplinary descriptions such as artist-teacher, teaching-artist, etc. The hyphenated association has become postmodern shorthand for inclusive “both/and” professional identities that in the 21st century may be limiting …

Publish And Flourish, Stephen K. Taylor

Faculty Publications

At primarily undergraduate institutions (PUIs), teaching is usually the primary responsibility, and many PUI professors do not regularly produce publications, even if they were hired to include research as an important responsibility. Most instructors at these schools are busy enough with just teaching and other academic responsibilities. It can seem intimidating to think of doing research, writing a research proposal, then a publication, and so on. Perhaps the sum of these duties may seem like a daunting set of tasks, and a "fear factor," consciously or subconsciously, may slow or even stop the research initiative. But at PUIs, it is …

The University Of Texas Of The Permian Basin Summer 2012 Course Schedule, The University Of Texas Of The Permian Basin

Course Schedules

No abstract provided.

Contribution Of The Infection-Associated Complement Regulator-Acquiring Surface Protein 4 (Erpc) To Complement Resistance Of Borrelia Burgdorferi, Claudia Hammerschmidt, Teresia Hallström, Christine Skerka, Reinhard Wallich, Brian Stevenson, Peter F Zipfel, Peter Kraiczy

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Borrelia burgdorferi evades complement-mediated killing by interacting with complement regulators through distinct complement regulator-acquiring surface proteins (CRASPs). Here, we extend our analyses to the contribution of CRASP-4 in mediating complement resistance of B. burgdorferi and its interaction with human complement regulators. CRASP-4 (also known as ErpC) was immobilized onto magnetic beads and used to capture proteins from human serum. Following Western blotting, factor H (CFH), CFH-related protein 1 (CFHR1), CFHR2, and CFHR5 were identified as ligands of CRASP-4. To analyze the impact of native CRASP-4 on mediating survival of serum-sensitive cells in human serum, a B. garinii strain was generated …

Influence Of Perceived Racial Discrimination On Health And Behaviour Of Immigrant Children In British Columbia, Anne George, Cherylynn Bassani, Robert Armstrong

Paediatrics and Child Health, East Africa

This study examines the influence of perceived discrimination on the health and behaviour of ethnic minority immigrant children in British Columbia, Canada. Using data from the New Canadian Children and Youth Study, we examine perceived discrimination experienced by the parent, family, and cultural group in Canada to test the influence of micro-, meso-, and macrolevels of discrimination on children. Families from 6 ethnic backgrounds participated in the study. Parents’ perceptions of the child’s health and six behavioral scales (hyperactivity, prosocial behaviour, emotional problems, aggression, indirect aggression, and a general combined behaviour scale) were examined as outcome variables. After controlling for …

Combination Effects Of Docetaxel And Doxorubicin In Hormone-Refractory Prostate Cancer Cells, Eleftheria Tsakalozou, Allison M. Eckman, Younsoo Bae

Pharmaceutical Sciences Faculty Publications

Combination effects of docetaxel (DOC) and doxorubicin (DOX) were investigated in prostate cancer cells (PC3 and DU145). Combination indices (CIs) were determined using the unified theory in various concentrations and mixing ratios (synergy: CI < 0.9, additivity: 0.9 < CI < 1.1, and antagonism: CI > 1.1). DOC showed a biphasic cytotoxicity pattern with the half maximal inhibitory concentration (IC50) at the picomolar range for PC3 (0.598 nM) and DU145 (0.469 nM), following 72 h drug exposure. The IC50s of DOX were 908 nM and 343 nM for PC3 and DU145, respectively. Strong synergy was seen when PC3 was treated with DOC at concentrations lower than its IC50 values (0.125~0.5 nM) plus …

Ceria-Engineered Nanomaterial Distribution In, And Clearance From, Blood: Size Matters, Mo Dan, Peng Wu, Eric A. Grulke, Uschi M. Graham, Jason M. Unrine, Robert A. Yokel

Pharmaceutical Sciences Faculty Publications

AIMS: Characterize different sized ceria-engineered nanomaterial (ENM) distribution in, and clearance from, blood (compared to the cerium ion) following intravenous infusion.

MATERIALS & METHODS: Cerium (Ce) was quantified in whole blood, serum and clot (the formed elements) up to 720 h.

RESULTS: Traditional pharmacokinetic modeling showed best fit for 5 nm ceria ENM and the cerium ion. Ceria ENMs larger than 5 nm were rapidly cleared from blood. After initially declining, whole blood 15 and 30 nm ceria increased (results that have not been well-described by traditional pharmacokinetic modeling). The cerium ion and 5 and 55 nm ceria did not …

The Hybrid Laboratory: Informal Spaces For Public-Science Interaction, Whitney Ann Ferrin-Rodriguez

Master's Theses

While growing trends in participatory science and public outreach have made science more accessible to publics, science communities and publics have long interpreted science and laboratories as private domains, perpetuating the idea that science is reserved for "experts" and locations for science are deemed "private." Many studies that examine public-science interaction, however, seldom consider the role of space and place. I argue that material spaces shape public-science interactions. My analysis takes place at an institutionalized informal education platform: a hybrid laboratory: a space used by "expert" astronomers to create new visual knowledge and "lay" publics to actively participate in astronomy. …

Influence Of Primary Care Physician Availability And Socioeconomic Deprivation On Breast Cancer From 1988 To 2008: A Spatio-Temporal Analysis, Lung-Chang Chien, Anjali D. Deshpande, Donna B. Jeffe, Mario Schootman

Open Access Publications

No abstract provided.