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Successful Genotyping Of Microsatellites In The Woolly Mammoth, Yasuko Ishida, Alfred L. Roca, Stephen Fratpietro, Alex D. Greenwood

Biological Sciences Faculty Publications

Genetic analyses using ancient DNA from Pleistocene and early Holocene fossils have largely relied on mitochondrial DNA (mtDNA) sequences. Among woolly mammoths, Mammuthus primigenius, mtDNA analyses have identified 2 distinct clades (I and II) that diverged 1-2 Ma. Here, we establish that microsatellite markers can be effective on Pleistocene samples, successfully genotyping woolly mammoth specimens at 2 loci. Although significant differentiation at the 2 microsatellite loci was not detected between 16 clade I and 4 clade II woolly mammoths, our results demonstrate that the nuclear population structure of Pleistocene species can be examined using fast-evolving nuclear microsatellite markers.

Turning Over A New Sprout: Promoting Agricultural Health By Fostering The Coexistence Of Organic And Genetically Modified Crops In The Wake Of Monsanto Co. V. Geertson Seed Farms And The Deregulation Of Modified Alfalfa, Joseph Kiefer

Emory Law Journal

According to the United States Department of Agriculture (USDA), the agricultural health of the United States requires the concurrent feasibility, or coexistence, of organic crops and genetically modified crops. Both types of crops offer separate environmental, economic, and health benefits. Modified crops, or crops infused with beneficial genes to increase yield or decrease the need for chemical applications, are ubiquitous in U.S. farming. Similarly, organic crops, or crops grown without modified genes, are gaining popularity. Unfortunately, the coexistence of organic and modified crops is threatened by the phenomenon of gene flow.

Biblical Reflections On Co-Creating With The Redeemer, William Kurz, Anne Carpenter

Theology Faculty Research and Publications

The Bible does not explicitly answer questions about co-creating with God and discerning whether to try to have children. In consulting Scripture regarding contemporary concerns, one needs to go beyond historical exegesis. Reading Scripture as God's Word requires seeking what God, the divine author of all of Scripture, is currently saying in the biblical passages under study.

The primary foundation for biblical teaching about marriage and family is Genesis, especially concerning God's original intention in creating marriage (Gen 1-2). Humans are created in the image of God as male and female, and marriage is the two becoming one flesh. Most …

Hearer Of The Triune God: Martin Luther's Reading Of Noah, Mickey Mattox

Theology Faculty Research and Publications

No abstract provided.

Reflections On The Reception Of The Church Fathers In The Contemporary Context, Marcus Plested

Theology Faculty Research and Publications

No abstract provided.

Proportionality's Cultural Foundation, Jordan M. Singer

Santa Clara Law Review

No abstract provided.

Arboretum News, Armstrong State University

Armstrong News & Publications: Arboretum News (Archived)

A Newsletter of the Armstrong Atlantic State University Arboretum

Patterns Of Gene Expression In Microarrays And Expressed Sequence Tags From Normal And Cataractous Lenses, Konstantinos Sousounis, Panagiotis A. Tsonis

Biology Faculty Publications

In this contribution, we have examined the patterns of gene expression in normal and cataractous lenses as presented in five different papers using microarrays and expressed sequence tags. The purpose was to evaluate unique and common patterns of gene expression during development, aging and cataracts.

Lens Regeneration In Axolotl: New Evidence Of Developmental Plasticity, Rinako Suetsugu-Maki, Nobuyasu Maki, Saulius Sumanas, Jie Zhu, Katia Del Rio-Tsonis, Panagiotis A. Tsonis

Biology Faculty Publications

Background: Among vertebrates lens regeneration is most pronounced in newts, which have the ability to regenerate the entire lens throughout their lives. Regeneration occurs from the dorsal iris by transdifferentiation of the pigment epithelial cells. Interestingly, the ventral iris never contributes to regeneration. Frogs have limited lens regeneration capacity elicited from the cornea during pre-metamorphic stages. The axolotl is another salamander which, like the newt, regenerates its limbs or its tail with the spinal cord, but up until now all reports have shown that it does not regenerate the lens.

Conservation Of The Three-Dimensional Structure In Non-Homologous Or Unrelated Proteins, Konstantinos Sousounis, Carl E. Haney, Jin Cao, Bharath Sunchu, Panagiotis A. Tsonis

Biology Faculty Publications

In this review, we examine examples of conservation of protein structural motifs in unrelated or non-homologous proteins. For this, we have selected three DNA-binding motifs: the histone fold, the helix-turn-helix motif, and the zinc finger, as well as the globin-like fold. We show that indeed similar structures exist in unrelated proteins, strengthening the concept that three-dimensional conservation might be more important than the primary amino acid sequence.

Potential Wolverine Habitat Vs. Winter Recreation. Conflict In Colorado!, Paul Quigley

Geography and the Environment: Graduate Student Capstones

The Global list of endangered species of flora and fauna is growing, with the most highly specialized species often at ‘critically endangered’ status. Managing these populations effectively involves numerous and varied organizations, conflicting motivations, arbitrary anthropogenic boundaries and often most importantly, data compilation and management. We are seeing many more reintroductions of locally extirpated species back into habitats of historical prevalence – and as extreme a method of conservation as this is, there is still a need for more extreme methods. High profile and highly specialized endangered species are often managed in a ‘crisis’ mode, where complex behaviors and interactions …

The Apoptotic Analysis Of 7Α-Hydroxy-Β-Sitosterol Extracted From Chisocheton Tomentosus (Meliaceae) In Cancer Cell Lines / Mohammad Tasyriq Bin Che Omar, Mohammad Tasyriq Che Omar

Student Works (2010-2019)

The main objective of the present study is to investigate the cytotoxicity potential and anti-cancer mechanism of 7α-hydroxy-β-sitosterol (CT1), a known stigmastane sterol extracted from bark of Chisocheton tomentosus (Meliaceae). In vitro exposures of this compound was conducted on five cancer cell lines; breast adenocarcinoma cells (MCF-7), hepatocyte liver carcinoma cell (HepG2), oral squamous carcinoma cell (HSC-4) and (HSC-2) and epidermoid cervical carcinoma (Ca Ski) and in comparison with normal human mammary epithelia cell line (HMEC). Cell viability was assessed by the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay and Live/Dead cytotoxic/viability assay. The flow cytometric analysis and DNA fragmentation assays were used …

Evaluation Of Superworm Meal (Zophobas Morio) As A Dietary Protein Source For Red Tilapia (Oreochromis Sp.) Diet / Abd Rahman Jabir Mohd Din, Mohd Din Abd Rahman Jabir

Student Works (2010-2019)

The aim for this research was to develop a cost-effective diet for red tilapia (Oreochromis spp.) using an insect protein-based source, the super worm meal (SWM), Zophobas morio according to various levels of fish meal replacement. This research would be focused on alternative protein source to fish meal with some assessment needed for a complete diet. This experiment consists of 4 experiments. Firstly, the nutritional evaluation and proximate analysis of Z. morio meal in Oreochromis spp. diet were studied. Then, the SWM was partially replaced by appropriate substitution level before being enriched by methionine and then supplemented by mushroom stalk …

Identification And Evaluation Of Chemical Constituents Of Pleurotus Sajor-Caju (Fr.) Singer Extracts In Lipid Metabolism And Glucose Homeostasis / Gowri A/P Kanagasabapathy, Kanagasabapathy Gowri

Student Works (2010-2019)

Mushrooms have been valued throughout the world as both food and medicine for thousands of years. Pleurotus sajor-caju has become attractive as functional food and a source of physiologically beneficial substances. These functional characteristics are mainly due to their chemical composition thus the chemical compositions of P. sajor-caju were investigated in this study. Twenty-seven compounds have been separated and identified from the EAE and BE extracts of P. sajor-caju. EAE comprised of three methyl esters namely, methyl palmitate, methyl stearate and methyl linoleate, five hydrocarbon fatty acids namely, pentadecanoic acid, palmitic acid, oleic acid, myristic acid and linoleic acid, a …

Postharvest Physiology And Molecular Characterization Of Ethylene Related Genes In Pollinated Dendrobium Pompadour / Zuliana Razali, Zuliana Razali

Student Works (2010-2019)

Pollination-induced senescence in Dendrobium Pompadour flowers was investigated. Pollinated flowers were held in distilled water and in treatment solutions containing either sucrose, glucose, aminooxyacetic acid (AOA) or silver thiosulphate (STS) while unpollinated flowers were held in distilled water. Morphological changes were observed visually while physiological parameters investigated included ethylene production, colour change, petal thickness, fresh weight, dry weight and water uptake. Biochemical changes determined were starch, total sugar and reducing sugar, cell wall hydrolases, total protein, soluble and insoluble protein. Furthermore, 1D SDS PAGE was also carried out to profile the protein changes in all the flowers. Finally five genes; …

Cytoarchitectonic Characterisation Of Rice-Field Frog (Fejervarya Limnocharis) Spinal Cord With Emphasis On Immunohistochemical Localisation Of Selected Nociceptive-Related Neurotransmitters / Joan Tang May Yin, Joan May Yin Tang

Student Works (2010-2019)

No abstract provided.

Physico-Chemical Characterization Of Branched-Chain Glycolipids For Drug Delivery System / Noraini Binti Ahmad, Noraini Ahmad

Student Works (2010-2019)

No abstract provided.

Chemometric Approaches In The Evaluation Of Trace Metals In Commercially Raised Tilapia And Preliminary Health Risk Assessment Of Its Consumption / Low Kah Hin, Kah Hin Low

Student Works (2010-2019)

The aims of this study are to evaluate the presence of trace metals in aquacultural samples using chemometric methods and to assess the safety of their consumption. In this work, chemometric techniques have been utilized to improve the analysis of metal content in commercially raised tilapia. In the method development phase, multivariate calibration techniques involving principal component regression and partial least squares were employed to establish a simple spectrophotometric method for the simultaneous determination of Cu2+, Ni2+ and Zn2+ in water samples in the presence of 1-(2-thiazolylazo)-2-naphthol as chromogenic reagent. In general, no significant difference in analytical performance was observed …

Potential Of Chrysomya Megacephala (Diptera:Calliphoridae) Maggot Meal As Protein Source In Tilapia (Oreochromis Sp.) Feed / Sing Kong Wah, Kong Wah Sing

Student Works (2010-2019)

Currently, fishmeal is a common protein source in aquafeed for farmed fish. However, the demand for fishmeal is increasing but supply is stagnating or even decreasing and therefore is insufficient to meet demand. This has caused the increase of fishmeal price in global markets and thereby, incurs higher production costs. Thus, there is an urgent need to find a cheaper but suitable protein source to replace fishmeal in animal feed. The objective of this study is to evaluate the potential of Chrysomya megacephala (Fabricius, 1974) maggot meal as protein source in red tilapia (Oreochromis sp.) feed. Protein content of blowfly …

Chemical Constituents Of Cuscuta Campestris Yuncker / Leong Sow Tein, Sow Tein Leong

Student Works (2010-2019)

Cuscuta campestris Yuncker (Golden Dodder) is commonly known as Rumput Emas in Malaysia. Cuscuta campestris is a weed which has become a serious threat to agriculture. A phytochemical study on the chemical constituents of sample (C. campestris) was performed. The sample was collected along the roadside at Pekan Nanas, Pontian, Johore (N 1° 20’ to N 2° 35’ - E 102° 28’ 59.9” to E 104° 33’ 52.9”). After separation from host plants, the sample was dried and extracted with ethanol. Chromatography method was used in separation and isolation of chemical constituents of sample. Six compounds were isolated viz., sitosterol …

A Study Of Nuclear Track Counting And Granulometry For Monazite: A Comparision Of The Sdc Morphology Toolbox And Image Processing Toolbox / Naharudin Mustafa, Mustafa Naharudin

Student Works (2010-2019)

The main aim of this work is to establish a technique to prepare the image of nuclear track detector in the form of thin film, LR 115 Type 2. This research also aims to develop the image processing scripts using SDC Morphology toolbox and Image Processing toolbox in conjunction with MATLAB as a programming platform. The scripts were applied to find the segmentation limit of over-lapping nuclear tracks, nuclear tracks counting and granulometry (area and diameter determination) in the images obtained. We finally compare the toolboxes capability in processing the image. The research found that the SDC Morphology toolbox is …

Thermal Performance And Economic Analysis Of Solar Photovoltaic Water Heater Under The Malaysian Climatic Condition / Md. Shouquat Hossain, Hossain Md. Shouquat

Student Works (2010-2019)

The solar energy is one of the highest reliable renewable energy resources. Solar water heater (SWH) system as a considerable application of solar energy has been studied by many researchers. There have been several innovations in the design of SWH through decades of research, and still there is a significant potential to improve the efficiency and optimize the cost of SWH through new and innovative designs. In this study, the modified design of the solar energy absorber of flat plate collector through a water heating system has achieved a commendable success experimentally. The Hottel- Whillier-Bliss equation and Annual Worth method …

Occupational Physical Activity Measurement Among Nurses In Universiti Malaya Medical Centre / Syamsul Rizal Bin Abu Amin, Abu Amin Syamsul Rizal

Student Works (2010-2019)

Although many studies have investigated the correlates of physical activity, these were mostly conducted in the leisure time domain. There is still lack of understanding about correlates physical activity exclusively in the workplace setting, as most study in the occupational domain are coupled with transportation domain. This study objectively examined the correlates of physical activity only in the occupational setting, where different backgrounds of female nurses were examined within the similar physical, social and working environment. Objectives: The primary objective is to examine relationships between correlates and components of physical activity in occupational setting. Secondary objectives are to investigate the …

Molecular Characterization Of Multidrug Resistant Pseudomonas Aeruginosa / Abdelkodose Mohammed Hussen Abdulla, Abdelkodose Mohammed Hussen Abdulla

Student Works (2010-2019)

Pseudomonas aeruginosa is one of the main causes of healthcare-associated infections among hospitalized patients. Healthcare-associated infections predominantly lead to pneumonia, urinary tract infections, as well as skin and soft-tissue infections. This organism is commonly multiresistant and leads to morbidity and mortality. In this study, the resistance mechanisms of 88 clinical P. aeruginosa isolates from the Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia were evaluated. The antibiotics resistance profiles of these isolates were determined and this was followed by evaluating the expression levels of AmpC cephalosporinase, the multidrug efflux pumps, the OprD outer membrane porin …

A Preliminary Study Of Neuregulin 1 Gene Expression In Schizophrenia Patients On Paliperidone In A Malaysian Population In University Malaya Medical Centre (Ummc) / Nazariah Aiza Harun, Harun Nazariah Aiza

Student Works (2010-2019)

BACKGROUND: The Neuregulin 1gene (NRG 1) polymorphism has been considered as a susceptibility gene for Schizophrenia. This is evident from studies done in several European and Chinese populations that suggesting a strong association between NRG 1 gene and Schizophrenia. There are numerous related studies worldwide and in Asian region but not in Malaysia that consists of 3 major races. Therefore, it is important for us to understand the reason for measuring NRG 1 gene expression given the importance of the gene as a risk factor for Schizophrenia, suggesting that NRG1 may have the potential as a biomarker for schizophrenia. OBJECTIVE: …

The Role Of Gelam Honey In Modulating The Immune Response Of Sepsis In Animal Model / Mustafa Kassim, Kassim Mustafa

Student Works (2010-2019)

Persistent systemic inflammatory response syndrome is a serious health condition that may lead to multiple organ dysfunction, organ failure, and ultimately death. It leads to both acute inflammation, caused by either infective (microbes or lipopolysaccharide [LPS]) or non-infective (chemicals) sources, and sepsis, an infection caused by a lethal dose of LPS (endotoxemia). These conditions have similar inflammatory mediators such as cytokines, nitric oxide (NO), high-mobility group box-1 (HMGB1), and heme oxygenase-1 (HO-1), suggesting that they may result from similar pathogenic mechanisms. Previous studies have investigated the applications of natural products in targeting these inflammatory mediators. Honey, for example, is used …

Occupational Physical Activity Measurement Among Nurses In Universiti Malaya Medical Centre / Dr. Syamsul Rizal Bin Abu Amin, Abu Amin Syamsul Rizal

Student Works (2010-2019)

Background: Although many studies have investigated the correlate of physical activity, these were mostly conducted in the leisure time domain. There is still lack of understanding about correlates physical activity exclusively in the workplace setting, as most study in the occupational domain are coupled with transportation domain. This study objectively examined the correlates of physical activity only in the occupational setting, where different backgrounds of female nurses were examined within the similar physical, social and working environment. Objectives: The primary objective is to examine relationships between correlates and component of physical activity in occupational setting. Secondary objectives are to investigate …

Effect Of Leech Therapy On Body Composition Parameters / Nazihah Ahmad, Ahmad Nazihah

Student Works (2010-2019)

Leech therapy treatment is one of the complimentary and alternative medicine that has been categorized as one of the ancient method in treating diseases. Locally unpopular this treatment still has its own believer that practices it for the sake of bloodletting treatment that claims to be successful. Therefore, a series of study should be conducted in order to prove the aforementioned claim by using the bioimpedance analysis. Bioimpedance analysis is way of measuring the fat and fat free mass in much more convenience way. This measurement is normally used by nutritionist as this invasive method is very suitable to determine …

Effect Of Acupuncture Therapy On Body Composition Parameters / Kamrun Nahar, Nahar Kamrun

Student Works (2010-2019)

Acupuncture is a technique for inserting needles into the body to introduce anesthesia or reduce pain. In detail, acupuncture basically is a procedure involved with stimulation of anatomical locations on or in the skin by using different types of techniques. Acupuncture is used for maintaining condition of body or treats human diseases for thousands of years.This thesis concerns the bioimpedance parameters (BI) and body composition parameters of people before and after undergoing acupuncture therapy. To measure bioimpedance (BI) parameters of normal subject and patients before and after acupuncture therapy I need to calculate the body composition parameters using Quadscan 4000. …

Improvement Of Dielectrophoretic Data Analysis Algorithm In Determining The Relative Polarisability Of Dielectric Particles / Robiyanti Adollah, Adollah Robiyanti

Student Works (2010-2019)

In the past few years, there has been an extensive research in the manipulation and analysis of biological cells at the micro scale. DEP offer the ability to manipulate particles, especially living cells, is fundamental to many biological and medical applications, including isolation and detection of cancer cells, concentration of cells from dilute suspensions, separation of cells according to specific properties, and trapping and positioning of individual cells for characterization. The term DEP was first introduced by Pohl in 1951 to describe the transnational motion of particles due to the application of non-uniform electrical fields. The dielectrophoretic motion is determined …