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2011

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Electrophoretic Analysis Of Genetic Diversity In Natural House Fly (Musca Domestica L.) Populations From The Western And Southern Coasts Of Turkey, Belgi̇n Göçmen Taşkin, Vatan Taşkin, Köksal Küçükakyüz Jan 2011

Electrophoretic Analysis Of Genetic Diversity In Natural House Fly (Musca Domestica L.) Populations From The Western And Southern Coasts Of Turkey, Belgi̇n Göçmen Taşkin, Vatan Taşkin, Köksal Küçükakyüz

Turkish Journal of Biology

In this study genetic variation was determined with isozyme analysis in 8 natural house fly (Musca domestica L.) populations from the western and southern coasts of Turkey, a part of the southern Palearctic region. For this purpose, 7 isozyme systems (ACP, AOX, CAT, HBDH, HXK, MDH, and SOD) were resolved using the native polyacrylamide gel electrophoresis (native-PAGE) technique. A total of 9 loci and 20 alleles were detected. The proportion of polymorphic loci in populations ranged from 44.44% to 66.67%. The overall mean number of effective alleles per locus (Ne) was 1.314 ± 0.454. The mean estimated expected heterozygosity (He) …

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The Analgesic Effect Of The Ethanolic Extract Of Matricaria Aurea, Esam Qnais Jan 2011

The Analgesic Effect Of The Ethanolic Extract Of Matricaria Aurea, Esam Qnais

Turkish Journal of Biology

The analgesic effect of the ethanolic whole plant extract of Matricaria aurea L. (Asteraceae) was studied in rats using the cold water tail flick assay and in mice using the tail immersion, tail clip, acetic-acid-induced writhing, and formalin pain tests. The results showed dose-dependent and significant (P < 0.001) increases in pain threshold at 60 min post-treatment with doses of 100, 200, and 300 mg/kg of the extract in tail flick, tail immersion, and tail clip tests. The effects of the extract were significantly (P < 0.001) lower than those produced by morphine (10 mg/kg) in the same tests. The extract (100, 200, and 300 mg/kg) exhibited a dose-dependent inhibition of writhing and also showed a significant (P < 0.001) inhibition in both phases of the formalin pain test, but with a less intense effect in the first phase. The results indicate that the analgesic effect of M. aurea ethanolic extract is both centrally and peripherally mediated.

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Total Phenolic Contents And Antioxidant Activities Of Prangos Lindl. (Umbelliferae) Species Growing In Konya Province (Turkey), Jehad Ahmed, Ayşegül Güvenç, Nurgün Küçükboyaci, Ayşe Baldemi̇r, Maksut Coşkun Jan 2011

Total Phenolic Contents And Antioxidant Activities Of Prangos Lindl. (Umbelliferae) Species Growing In Konya Province (Turkey), Jehad Ahmed, Ayşegül Güvenç, Nurgün Küçükboyaci, Ayşe Baldemi̇r, Maksut Coşkun

Turkish Journal of Biology

Prangos species are widely used as medicinal plants in Turkey, and 14 species of this genus grow naturally in Anatolia. In the present study, the phenolic contents and antioxidant activities of the water and methanol (MeOH) extracts obtained from the root, herb, and fruits of 4 species of Prangos (Prangos ferulacea, P. heyniae, P. meliocarpoides var. meliocarpoides, and P. uechtritzii) collected in Konya province were compared. The phenolic contents of the samples were determined using Folin-Ciocalteu's phenol reagent. Antioxidant activities of the extracts were studied by qualitative DPPH^. (1,1-diphenyl-2-picrylhydrazyl radical) assay to detect the free radical scavenging activity and by …

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Determination Of Antimicrobial And Antiproliferative Activities Of The Aquatic Moss Fontinalis Antipyretica Hedw., Fi̇li̇z Savaroğlu, Cansu Fi̇li̇k İşçen, Ayşe Pinar Öztopcu Vatan, Selda Kabadere, Semra İlhan, Ruhi̇ Uyar Jan 2011

Determination Of Antimicrobial And Antiproliferative Activities Of The Aquatic Moss Fontinalis Antipyretica Hedw., Fi̇li̇z Savaroğlu, Cansu Fi̇li̇k İşçen, Ayşe Pinar Öztopcu Vatan, Selda Kabadere, Semra İlhan, Ruhi̇ Uyar

Turkish Journal of Biology

The purpose of the current study was to investigate the possible antimicrobial and anticancer properties of the aquatic moss Fontinalis antipyretica Hedw. (Fontinalaceae). We first obtained 8 different extracts (methanol; chloroform; acetone; ethyl acetate A, B, C, and D) by two different extraction processes. The antimicrobial activity of these extracts was then assessed by using the well diffusion method against 8 bacterial and 7 fungal strains. The results of antimicrobial studies showed that extracts of chloroform, acetone, and ethyl acetate A and C were active against almost all the species tested. The most effective extract was C, which was then …

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Levels Of Phenolic Compounds And Their Effects On Antioxidant Capacity Of Wild Vaccinium Arctostaphylos L. (Qare-Qat) Collected From Different Regions Of Iran, Tahereh Hasanloo, Roshanak Sepehrifar, Homa Hajimehdipoor Jan 2011

Levels Of Phenolic Compounds And Their Effects On Antioxidant Capacity Of Wild Vaccinium Arctostaphylos L. (Qare-Qat) Collected From Different Regions Of Iran, Tahereh Hasanloo, Roshanak Sepehrifar, Homa Hajimehdipoor

Turkish Journal of Biology

Antioxidant activities in the leaves and fruits of wild Vaccinium arctostaphylos (locally named Qare-Qat) from different regions of Iran were evaluated using the stable 1, 1-diphenyl-2-picrylhydrazyl free radical and ferric reducing/antioxidant power methods. Variations in antioxidant activity, total phenolic, anthocyanin, and flavonoid content in fruit samples among genotypes was much greater than the variation observed between growing seasons, indicating that genetics plays a more important role than growing season in V. arctostaphylos fruits. The data indicated that the fruits of the Kelardasht genotype had the highest total phenolic content [42.73 ± 1.5 mg gallic acid equivalents (GAE) g^{-1} DW], anthocyanin …

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Characterisation And Antimicrobial Activity Of Sophora Alopecuroides L. Var. Alopecuroides Alkaloid Extracts, Nurgün Küçükboyaci, Semi̇ha Özkan, Nezaket Adigüzel, Fatma Tosun Jan 2011

Characterisation And Antimicrobial Activity Of Sophora Alopecuroides L. Var. Alopecuroides Alkaloid Extracts, Nurgün Küçükboyaci, Semi̇ha Özkan, Nezaket Adigüzel, Fatma Tosun

Turkish Journal of Biology

The aerial parts and seeds of Sophora alopecuroides L. var. alopecuroides growing in Turkey were investigated for their alkaloid compositions and antimicrobial activities. The alkaloid extracts were analysed by capillary gas chromatography-mass spectrometry (GC-MS). The main components were identified as sophoridine (33.07%), matrine (24.03%), and sophocarpine (8.01%) in the aerial parts, and matrine (36.67%) and sophoridine (34.53%) in the seeds of the plant. The alkaloid extracts were also evaluated for their in vitro antibacterial and antifungal activities using microdilution techniques. The alkaloid extract of the aerial parts of S. alopecuroides var. alopecuroides presented significant activity against Staphylococcus aureus and Bacillus …

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Cover And Contents Jan 2011

Cover And Contents

Turkish Journal of Biology

No abstract provided.

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Cover And Contents Jan 2011

Cover And Contents

Turkish Journal of Biology

No abstract provided.

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Molecular Cloning, Characterization, And Expression Analysis Of A Gene Encoding A Ran Binding Protein (Ranbp) In Cucumis Melo L., Mehmet Cengi̇z Baloğlu, Florence Negre-Zakharov, Hüseyi̇n Avni̇ Öktem, Ayşe Meral Yücel Jan 2011

Molecular Cloning, Characterization, And Expression Analysis Of A Gene Encoding A Ran Binding Protein (Ranbp) In Cucumis Melo L., Mehmet Cengi̇z Baloğlu, Florence Negre-Zakharov, Hüseyi̇n Avni̇ Öktem, Ayşe Meral Yücel

Turkish Journal of Biology

Ran binding proteins (RanBPs) are highly conserved members of the GTP-binding protein family that are involved in nuclear protein export between the nucleus and the cytoplasm. In this study, a CmRanBP gene from a melon was isolated (Cucumis melo L.) using the RACE (rapid amplification of cDNA ends) method. The 778 basepair long melon, with a RanBP cDNA encoding consisting of 197 amino acids (22.2 kDa protein), was characterized (GenBank accession no: EU853459). The predicted amino acid sequence of CmRanBP was found to be 70% identical to VvRanBP, PtRanBP, and RcRanBP from Vitis vinifera, Populus trichocarpa, and Ricinus communis, respectively. …

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Testing The Putative Effect Of Dicer-Substrate Sirnas In Regulating Gene Expression At Transcriptional Level, Müge Kovancilar, Mehmet Nejat Dalay, Uğur Deli̇gezer Jan 2011

Testing The Putative Effect Of Dicer-Substrate Sirnas In Regulating Gene Expression At Transcriptional Level, Müge Kovancilar, Mehmet Nejat Dalay, Uğur Deli̇gezer

Turkish Journal of Biology

RNA interference (RNAi) pathway is a gene silencing process during which small double-stranded RNA (dsRNA) molecules trigger the degradation of homologous RNA targets. Small interfering RNAs (siRNAs) are the mediators of the RNAi that can be induced in vitro and in vivo by direct application of chemically synthesized siRNAs. Recently, promoter-targeted small non-coding RNAs have been described to be capable of regulating gene expression at the transcriptional level. In the present study we tested the hypothesis that Dicer-substrate siRNAs (D-siRNAs), which trigger gene silencing through intrinsic RNAi pathway and are therefore more potent in gene knockdown than traditionally used 21-23mer …

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The Application Of Issr-Pcr To Determine The Genetic Relationship And Genetic Diversity Between Narrow Leaved Bluegrass (Poa Angustifolia) And Rough Bluegrass (Poa Trivialis) Accessions, Emi̇ne Arslan, Ahmet Tamkoç Jan 2011

The Application Of Issr-Pcr To Determine The Genetic Relationship And Genetic Diversity Between Narrow Leaved Bluegrass (Poa Angustifolia) And Rough Bluegrass (Poa Trivialis) Accessions, Emi̇ne Arslan, Ahmet Tamkoç

Turkish Journal of Biology

: The present study aimed to determine the genetic relationship between Poa angustifolia and Poa trivialis as well as intraspecies genetic diversity using inter simple sequence repeat (ISSR) markers. ISSR amplification was evaluated for its applicability to narrow leaved bluegrass and rough bluegrass accessions identification. A total of 401 bands, 363 of which were polymorphic (90.52%), were generated using selected 20 primers. Genetic similarity between accessions belonging to Poa species was estimated using Nei's genetic similarity and UPGMA (unweighted pair group method with arithmetic) cluster analysis was used to construct a dendrogram. UBC836 and M6 primers were observed to be …

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Changes In The Hemolymph Total Protein Of Galleria Mellonella (Lepidoptera: Pyralidae) After Parasitism And Envenomation By Pimpla Turionellae (Hymenoptera: Ichneumonidae), Olga Sak, Ekrem Ergi̇n, Fevzi̇ Uçkan, David B. Rivers, Ayli̇n Er Jan 2011

Changes In The Hemolymph Total Protein Of Galleria Mellonella (Lepidoptera: Pyralidae) After Parasitism And Envenomation By Pimpla Turionellae (Hymenoptera: Ichneumonidae), Olga Sak, Ekrem Ergi̇n, Fevzi̇ Uçkan, David B. Rivers, Ayli̇n Er

Turkish Journal of Biology

Venom from the endoparasitoid Pimpla turionellae L. (Hymenoptera: Ichneumonidae) contains a mixture of biologically active components, which display potent paralytic, cytotoxic, and cytolytic effects towards hosts. Here, we further investigate if parasitism or envenomation by P. turionellae alters total protein of its host Galleria mellonella L. (Lepidoptera: Pyralidae). Various venom concentrations representing doses previously determined to yield host responses yet fall below the calculated LD99 were used for pupae and larvae. Parasitization was only assayed for host pupa since P. turionellae females normally parasitize host prepupae and pupae in nature. Hemolymph total protein concentration remained relatively steady at all doses …

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In Vitro Antagonistic Activity Of Fungi Isolated From Sclerotia On Potato Tubers Against Rhizoctonia Solani, Erkol Demi̇rci̇, Eli̇f Dane, Cafer Eken Jan 2011

In Vitro Antagonistic Activity Of Fungi Isolated From Sclerotia On Potato Tubers Against Rhizoctonia Solani, Erkol Demi̇rci̇, Eli̇f Dane, Cafer Eken

Turkish Journal of Biology

Forty-five fungal isolates were obtained from sclerotia of Rhizoctonia solani on potato tubers in Erzurum, Turkey. The interaction between fungal isolates and R. solani was studied in dual culture technique. Some fungal isolates affected R. solani by antibiosis and/or parasitism. Results of the antagonism tests showed that Acremonium sp., Gliocladium viride, Paecilomyces marquandii, Paecilomyces sulphurellus, Penicillium camemberti, Penicillium expansum, Penicillium frequentans (ME-50), Penicillium nigricans, Penicillium olsonii, Penicillium phialosporum, Sporothrix sp. (MCY-4), Sporothrix schenckii, and Verticillium dahliae isolates produced an inhibition zone in front of the R. solani colony to a varying degree. Trichoderma harzianum isolates were able to overgrow the …

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Biochemical Identification And Numerical Taxonomy Of Aeromonas Spp. Isolated From Food Samples In Turkey, Belgi̇n Erdem, Ergi̇n Kari̇ptaş, Eli̇f Çi̇l, Kami̇l Işik Jan 2011

Biochemical Identification And Numerical Taxonomy Of Aeromonas Spp. Isolated From Food Samples In Turkey, Belgi̇n Erdem, Ergi̇n Kari̇ptaş, Eli̇f Çi̇l, Kami̇l Işik

Turkish Journal of Biology

Numerical taxonomy was used to analyze phenotypic data obtained from 126 new isolates of Aeromonas strains taken from red meat, raw chicken, minced meat, and fish samples. Each strain was tested for 86 characters but only the final data including 63 characters were analysed using the S_{SM} coefficients and the UPGMA clustering algorithm. At S_{SM} values of => 83%, the strains clustered into 10 aggregate groups consisting of 7 major (5 and up strains) and 3 minor (2-4 strains), and 5 single member clusters, each of which was identified as A. hydrophila, A. caviae, and A. sobria, respectively. It was …

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Numerical Classification Of Streptomycetes Isolated From Karstic Caves In Turkey, Mustafa Yamaç, Kami̇l Işik, Nevzat Şahi̇n Jan 2011

Numerical Classification Of Streptomycetes Isolated From Karstic Caves In Turkey, Mustafa Yamaç, Kami̇l Işik, Nevzat Şahi̇n

Turkish Journal of Biology

In the present study, a total of 290 actinomycetes isolates were obtained from 19 karstic caves in Turkey. Out of these, 131 were chosen for numerical classification studies according to their morphology and pigmentation properties. Representative isolates were compared with the reference Streptomyces strains based on their various taxonomic characteristics such as biochemical, physiological, nutritional, and morphological, totalling 134 unit characters. The data were evaluated using simple matching coefficients (S_{SM}), and clustering achieved using the Complete algorithm by X-Taxon and NTSys-pc programs. The test strains were assigned to 9 major (5-11 strains), 18 minor (2-4 strains), and 31 single membered …

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Seasonal Variations In Phytoplankton Composition And Biomass In A Small Lowland River-Lake System (Melen River, Turkey), Tülay Baykal, İlkay Açikgöz, Abel U. Udoh, Kazim Yildiz Jan 2011

Seasonal Variations In Phytoplankton Composition And Biomass In A Small Lowland River-Lake System (Melen River, Turkey), Tülay Baykal, İlkay Açikgöz, Abel U. Udoh, Kazim Yildiz

Turkish Journal of Biology

A series of studies were carried out to determine the succession and phytoplankton community of the Melen River in the Western Black Sea region of Turkey. The study was conducted at 6 stations at monthly intervals between May 2003 and April 2004. It was observed that the abundance and biovolume of phytoplankton were low in winter, though higher values were recorded in late spring and summer. Among the values noted, the spring peak was 534.38 × 10^5 ind. L^{-1} and 7384.8 mm^3 L^{-1} belonging to Peridinium sp. The most common diatom, Cyclostephanos dubius, was favoured by low discharge at stations …

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Antibacterial Activity Of Bee Propolis Samples From Different Geographical Regions Of Turkey Against Two Foodborne Pathogens, Salmonella Enteritidis And Listeria Monocytogenes, Ayhan Temi̇z, Ayla Şener, Asli Özkök Tüylü, Kadri̇ye Sorkun, Beki̇r Sali̇h Jan 2011

Antibacterial Activity Of Bee Propolis Samples From Different Geographical Regions Of Turkey Against Two Foodborne Pathogens, Salmonella Enteritidis And Listeria Monocytogenes, Ayhan Temi̇z, Ayla Şener, Asli Özkök Tüylü, Kadri̇ye Sorkun, Beki̇r Sali̇h

Turkish Journal of Biology

The aim of the present study was to investigate the antibacterial activities of 25 propolis samples collected from various geographical regions of Turkey against 2 food-borne pathogens, Salmonella Enteritidis ATCC 13076 and Listeria monocytogenes ATCC 1462. The chemical compositions of ethyl alcohol extracts of the propolis (EEP) samples were determined by gas chromatography coupled to mass spectrometry. The main components of EEP samples were flavonoids, aromatic acid esters, aromatic alcohols, aromatic acids, aliphatic carboxylic acids, terpens, and aliphatic carboxylic acid esters. Antibacterial activities of the EEP samples were tested at 2 different dilutions of 1:10 and 1:100 (v/v). All EEP …

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Influence Of Different Sterilization Methods On Callus Initiation And Production Of Pigmented Callus In Arnebia Densiflora Ledeb., Hati̇ce Çölgeçen, Ufuk Koca Çalişkan, Gülnur Toker Jan 2011

Influence Of Different Sterilization Methods On Callus Initiation And Production Of Pigmented Callus In Arnebia Densiflora Ledeb., Hati̇ce Çölgeçen, Ufuk Koca Çalişkan, Gülnur Toker

Turkish Journal of Biology

We analyzed the effects of sodium hypochlorite and Plant Preservative Mixture (PPM) on surface sterilization. We also examined the effects that the addition of an antibiotic-antimycotic solution to the culture medium had on callus induction. Explants were initially sterilized with different concentrations of sodium hypochlorite and cultured on MS media containing kinetin (0.29 \muM) and naphthalene acetic acid (NAA, 10 \muM). No calluses were produced, either because of contamination of the explants, or loss of explants as a result of the high levels of sodium hypochlorite. The application of PPM and antibiotics at different concentrations reduced contamination and led to …

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The Interaction Between Renin-Angiotensin System And Sympathetic Nervous System In The Peripheral Vasculature Of Normal Sprague-Dawley Rats, Mohammed H. Abdulla, Munavvar A. Sattar, Nor A. Abdullah, Kolla Rl. Anand Swarup, Abdul Hye Khan, Edward J. Johns Jan 2011

The Interaction Between Renin-Angiotensin System And Sympathetic Nervous System In The Peripheral Vasculature Of Normal Sprague-Dawley Rats, Mohammed H. Abdulla, Munavvar A. Sattar, Nor A. Abdullah, Kolla Rl. Anand Swarup, Abdul Hye Khan, Edward J. Johns

Turkish Journal of Biology

To evaluate possible interactions between the renin-angiotensin system (RAS) and the sympathetic nervous system (SNS) in the systemic circulation upon the intravenous administration of angiotensin II and a set of adrenergic agonists in normal Sprague-Dawley (SD) rats. Rats were treated with carvedilol, a non-selective \beta- and selective \alpha_1-adrenoceptor blocker, in a dose of (5 mg/kg) orally for 7 days, and on day 8, the animals were pentobarbitone-anaesthetized and prepared for systemic hemodynamic study. Dose-response relationships in terms of elevation in the magnitude of the mean arterial blood pressure in response to intravenous injection of noradrenaline (NA), phenylephrine (PE), methoxamine (ME), …

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Cover And Contents Jan 2011

Cover And Contents

Turkish Journal of Biology

No abstract provided.

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Cover And Contents Jan 2011

Cover And Contents

Turkish Journal of Biology

No abstract provided.

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Isolation And Characterization Of Xylanolytic New Strains Of Anoxybacillus From Some Hot Springs In Turkey, Kadri̇ye İnan, Sabri̇ye Çanakçi, Ali̇ Osman Beldüz Jan 2011

Isolation And Characterization Of Xylanolytic New Strains Of Anoxybacillus From Some Hot Springs In Turkey, Kadri̇ye İnan, Sabri̇ye Çanakçi, Ali̇ Osman Beldüz

Turkish Journal of Biology

Some hot springs located in the west of Turkey were investigated regarding the presence of xylanolytic thermophilic microorganisms. Based on phenotyping characteristics and 16S rRNA gene sequence analysis, 9 of these xylanolytic isolates belonged to the genus Anoxybacillus, and grew optimally at about 50-60 °C on nutrient agar. The 16S rRNA gene sequence analysis showed that these isolates resembled the Anoxybacillus species =>97 and these isolates are members of the genus Anoxybacillus. Based on the DNA-DNA hybridization study, SDS-PAGE profile, and biochemical and physiological features, I3, CT1Sari, and BT2.1 isolates are new strains of Anoxybacillus gonensis; I4.2 and B9.3 …

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Isolation, Production, And Characterization Of An Extracellular Lipase From Trichoderma Harzianum Isolated From Soil, Serdar Ülker, Arzu Özel, Ahmet Çolak, Şengül Alpay Karaoğlu Jan 2011

Isolation, Production, And Characterization Of An Extracellular Lipase From Trichoderma Harzianum Isolated From Soil, Serdar Ülker, Arzu Özel, Ahmet Çolak, Şengül Alpay Karaoğlu

Turkish Journal of Biology

This is the first report about the characterization of Trichoderma harzianum lipase. A novel strain of Trichoderma harzianum IDM14D was isolated from soil. The isolated strain was cultivated for lipase production in shake flasks at 30 °C for 7 days. For lipase production, it was determined that the best carbon source was glucose and the best nitrogen source was peptone. Maximum biomass was produced at a concentration of 1.25 g/L, in 7 days. The optimum pH and temperature for activity of the enzyme were 8.5 and 40 °C, respectively. The lipase was stable at a pH range of 8.0-10.0 and …

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Assessment Of Potential Plastic-Degrading Fungi In Jordanian Habitats, Ibrahim N. Ibrahim, Anwar Maraqa, Khalid M. Hameed, Ismail M. Saadoun, Hamzah M. Maswadeh Jan 2011

Assessment Of Potential Plastic-Degrading Fungi In Jordanian Habitats, Ibrahim N. Ibrahim, Anwar Maraqa, Khalid M. Hameed, Ismail M. Saadoun, Hamzah M. Maswadeh

Turkish Journal of Biology

Out of 70 fungal isolates recovered from soil, wall paints (Latex), and pieces of plastic debris from different habitats in Jordan and plastic shields of street light posts on campus, 35 isolates showed varied potential to degrade polyester-polyurethane (PS-PUR). Six of these isolates (Fusarium solani, Alternaria solani, Spicaria spp., Aspergillus fumigatus, Aspergillus terreus, and Aspergillus flavus) were selected on the basis of their growth rates on basal salt media amended with PS-PUR as the sole source of carbon. Isolates were further evaluated utilizing 3 different methods: 1) direct plating, 2) clear zone in a 2-layered agar media, and 3) liquid …

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Acid Proteinase Enzyme Activity In Candida Albicans Strains: A Comparison Of Spectrophotometry And Plate Methods, Sevi̇m Akçağlar, Beyza Ener, Okan Töre Jan 2011

Acid Proteinase Enzyme Activity In Candida Albicans Strains: A Comparison Of Spectrophotometry And Plate Methods, Sevi̇m Akçağlar, Beyza Ener, Okan Töre

Turkish Journal of Biology

In recent years, the incidence of fungal infections has been rising all over the world. Although the amount of research in the field of pathogenic fungi has also increased, there is still a need for studies on fungal virulence. In this investigation, we focused on testing bloodstream-identified Candida albicans in experimental in vitro virulence assays. The proteinase enzyme activities of 30 C. albicans strains isolated from the bloodstream and sterile body fluids were investigated (15 colonizations, 15 infections). The spectrophotometric and plate methods were used to determine proteinase activity. The correlations between the 2 methods were compared. This study indicates …

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Characterization Of Poly Phenol Oxidase In Two In Vitro Regenerated Cultivars Of Mucuna: Mucuna Pruriens L. And Mucuna Prurita H., Raghavendra Sathyanarayana, Vadlapudi Kumar, Chapeyil Kumaran Ramesh, Mahadevappa Parmesha, Mahaboob Habeebulla Moinuddin Khan Jan 2011

Characterization Of Poly Phenol Oxidase In Two In Vitro Regenerated Cultivars Of Mucuna: Mucuna Pruriens L. And Mucuna Prurita H., Raghavendra Sathyanarayana, Vadlapudi Kumar, Chapeyil Kumaran Ramesh, Mahadevappa Parmesha, Mahaboob Habeebulla Moinuddin Khan

Turkish Journal of Biology

Polyphenol oxidases (PPOs EC 1.14.18.1) were isolated from Mucuna pruriens and Mucuna prurita and confirmed as tyrosinases involved in L-DOPA production. PPOs were extracted by using a 0.05 M phosphate buffer, pH 7.0. The purified enzyme was resolved into a single band by PAGE, the enzyme was confirmed as PPO by activity staining, and SDS-PAGE analysis revealed that the purified PPO of both the species is a tetramer. Substrate specificity experiments were carried out with catechol, L-DOPA, L-tyrosine, and p-cresol. Of these, catechol was evaluated as the most suitable substrate based on the determined K_{m} and V_{max} values. The optimum …

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In Vitro Propagation Of Diyarbakır Watermelons And Comparison Of Direct-Seeded And Transplanted Watermelon, Veysi̇ Okumuş, Vedat Pi̇ri̇nç, Ahmet Onay, Davut Başaran Jan 2011

In Vitro Propagation Of Diyarbakır Watermelons And Comparison Of Direct-Seeded And Transplanted Watermelon, Veysi̇ Okumuş, Vedat Pi̇ri̇nç, Ahmet Onay, Davut Başaran

Turkish Journal of Biology

A rapid protocol using shoot tip explants for micropropagation of Diyarbakır watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] types Sürme, Beyazkış and Karakış was achieved. Shoot tips from 5-day-old in vitro germinated seedlings were cultured on shoot regeneration medium for 3 weeks. The effects of the different concentrations of benzyl adenine (BA) and carbohydrate types on shoot proliferation were examined. The results confirmed that a range of 0.5-1.0 mg/L of BA was almost equally effective in promoting the shoot length of cultures in the 3 genotypes. Sucrose, in the presence of BA, was superior to other carbohydrates for the 3 …

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Antioxidant Capacity Of Juice From Different Papaya (Carica Papaya L.) Cultivars Grown Under Greenhouse Conditions In Turkey, Aysun Özkan, Hami̇de Gübbük, Esma Güneş, Ayşe Erdoğan Jan 2011

Antioxidant Capacity Of Juice From Different Papaya (Carica Papaya L.) Cultivars Grown Under Greenhouse Conditions In Turkey, Aysun Özkan, Hami̇de Gübbük, Esma Güneş, Ayşe Erdoğan

Turkish Journal of Biology

The fruits of Carica papaya L. (Caricaceae) are valuable as food and are also used in traditional medicine. The present study was designed to assess the antioxidant potential of the juices of 3 papaya cultivars (PCJ): Sunrise Solo, Red Lady, and Tainung. The antioxidant capacity of PCJ obtained from fully ripened fruit was determined by the following methods: scavenging of the free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH), reducing power assay, scavenging of superoxide radicals, 2-deoxyribose oxidation assay, and thiocyanate assay. Total phenolic content (TPC) of PCJ was determined by the Folin-Ciocalteu method. In the DPPH assay (IC_{50} values), the effect of Sunrise …

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Chemical Composition And Nutritional Value Of A Wild Edible Ectomycorrhizal Mushroom, Tricholoma Anatolicum, Erbi̇l Kalmiş, Hasan Yildiz, Bülent Ergönül, Fati̇h Kalyoncu, Mehmet Hali̇l Solak Jan 2011

Chemical Composition And Nutritional Value Of A Wild Edible Ectomycorrhizal Mushroom, Tricholoma Anatolicum, Erbi̇l Kalmiş, Hasan Yildiz, Bülent Ergönül, Fati̇h Kalyoncu, Mehmet Hali̇l Solak

Turkish Journal of Biology

The chemical composition and nutritional value of a wild edible ectomycorrhizal mushroom from southwestern Anatolia, Tricholoma anatolicum, were analyzed. Moisture, crude oil, protein, ash, total carbohydrate content, and mineral composition of the mushrooms studied, including Fe, Na, K, Zn, Cu, Ca, Cd, and Pb, were determined. The energy values of the samples were also calculated. The analyses were conducted during the 3 different growing stages of the mushrooms: mycelium, young fruiting bodies, and mature fruiting bodies. The highest values for moisture and crude oil contents were found to be in the mycelium, ash, and carbohydrate content of young fruiting bodies. …

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Biological Characterization Of Iranian Walnut (Juglans Regia) Leaves, Reza Sharafati Chaleshtori, Farhad Sharafati Chaleshtori, Mahmoud Rafieian Jan 2011

Biological Characterization Of Iranian Walnut (Juglans Regia) Leaves, Reza Sharafati Chaleshtori, Farhad Sharafati Chaleshtori, Mahmoud Rafieian

Turkish Journal of Biology

In recent years, emphasis has been placed on the use of natural materials in the control and treatment of various infections, as some chemically synthesized drugs have undesirable side effects. In this study, the ethanol extract of Iranian walnut leaves was examined for antibacterial activities on Streptococcus mutans, Streptococcus salivarius, Streptococcus sanguinis, and Actinomyces viscosus using the microdilution method. Total phenols, flavonoids, and flavonols were also determined colorimetrically. The minimum inhibitory concentrations (MIC) for ethanolic extract ranged between 15.6 and 187.5 mg/mL and minimum bactericidal concentrations (MBC) ranged between 31.25 and 250 mg/mL. Total phenols were 410 ± 14.43 mg/g …

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