Digital Commons Network^™

Phosphodiesterase-4 Inhibition Alters Gene Expression And Improves Isoniazid – Mediated Clearance Of Mycobacterium Tuberculosis In Rabbit Lungs, Selvakumar Subbian, Liana Tsenova, Paul O'Brien, Guibin Yang, Mi-Sun Koo, Blas Peixoto, Dorothy Fallows, Veronique Dartois, George Muller, Gilla Kaplan

Publications and Research

Tuberculosis (TB) treatment is hampered by the long duration of antibiotic therapy required to achieve cure. This indolent response has been partly attributed to the ability of subpopulations of less metabolically active Mycobacterium tuberculosis (Mtb) to withstand killing by current anti-TB drugs. We have used immune modulation with a phosphodiesterase-4 (PDE4) inhibitor, CC-3052, that reduces tumor necrosis factor alpha (TNF-α) production by increasing intracellular cAMP in macrophages, to examine the crosstalk between host and pathogen in rabbits with pulmonary TB during treatment with isoniazid (INH). Based on DNA microarray, changes in host gene expression during CC-3052 treatment of Mtb infected …

The Stress Factor: Exploring The Possibility Of A Psychological Component To Cancer, Bernard M. Edelstein, Md

Jefferson Journal of Psychiatry

As dwellers in the same house, it seems only logical that psyche and soma would be interdependent , that well-being in one would promote health in the other, and that illness in one would soon become manifest in its partner. Yet quantifying this intuitive relationship has long been a difficult task. Establishing a mechanism for disease-heart disease, infectious disease, neoplastic disease which can withstand the scrutiny of experimental rigor, is difficult enough. To introduce a seemingly not quantifiable entity such as psychology in the form of stress or anxiety presents such great complexity that for a long time it seemed …

Role Of Tolc Homologs In The Virulence Of Francisella Tularensis, Gabrielle Platz

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

Francisella tularensis is the causative agent of tularemia. There are five subspecies of F. tularensis, three that are pathogenic to humans. F. tularensis subsp. tularensis is extremely pathogenic and sometimes fatal. F. tularensis subsp. holarctica causes a less severe disease. The live vaccine strain (LVS) is derived from a holarctica strain and retains virulence in the mouse. F. tularensis subsp. novicida causes disease in immunocompromised persons. F. tularensis is classified as a Category A agent of iv bioterrorism by the Centers for Disease Control due to its low infectious dose, ease of aerosol dissemination and capacity to cause high morbidity …

Response Of Striped Bass To Immunological Stimulation And Challenge, Soo Jin Jeon

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

[to be added]

New Perspectives On The Role Of Vitiligo In Immune Responses To Melanoma, Katelyn T. Byrne, Mary Jo Turk

Dartmouth Scholarship

Melanoma-associated vitiligo is the best-studied example of the linkage between tumor immunity and autoimmunity. Although vitiligo is an independent positive prognostic factor for melanoma patients, the autoimmune destruction of melanocytes was long thought to be merely a side effect of robust anti-tumor immunity. However, new data reveal a key role for vitiligo in supporting T cell responses to melanoma. This research perspective reviews the history of melanoma-associated vitiligo in patients, the experimental studies that form the basis for understanding this relationship, and the unique characteristics of melanoma-specific CD8 T cells found in hosts with vitiligo. We also discuss the implications …

Gm-Csf Production Allows The Identification Of Immunoprevalent Antigens Recognized By Human Cd4+ T Cells Following Smallpox Vaccination, Valeria A. Judkowski, Alcinette Bunying, Feng Ge, Jon R. Appel, Kingyee Law, Atima Sharma, Claudia Raja-Gabaglia, Patricia Norori, Radleigh Santos, Marc Giulianotti, Mark K. Slifka, Daniel C. Douek, Barney S. Graham, Clemencia Pinilla

Mathematics Faculty Articles

The threat of bioterrorism with smallpox and the broad use of vaccinia vectors for other vaccines have led to the resurgence in the study of vaccinia immunological memory. The importance of the role of CD4+ T cells in the control of vaccinia infection is well known. However, more CD8+ than CD4+ T cell epitopes recognized by human subjects immunized with vaccinia virus have been reported. This could be, in part, due to the fact that most of the studies that have identified human CD4+ specific protein-derived fragments or peptides have used IFN-γ production to evaluate vaccinia specific T cell responses. …

Genome-Wide Comparison Of African-Ancestry Populations From Care And Other Cohorts Reveals Signals Of Natural Selection, Gaurav Bhatia, Nick Patterson, Bogdan Pasaniuc, Noah Zaitlen, Giulio Genovese, Samuela Pollack, Swapan Mallick, Simon Myers, Arti Tandon, Chris Spencer, Cameron D. Palmer, Adebowale A. Adeyemo, Ermeg L. Akylbekova, L. Adrienne Cupples, Jasmin Divers, Myriam Fornage, W. H.Linda Kao, Leslie Lange, Mingyao Li, Solomon Musani, Josyf C. Mychaleckyj, Adesola Ogunniyi, George Papanicolaou, Charles N. Rotimi, Jerome I. Rotter, Ingo Ruczinski, Babatunde Salako, David S. Siscovick, Bamidele O. Tayo, Qiong Yang, Steve Mccarroll, Pardis Sabeti, Guillaume Lettre

Faculty Publications

The study of recent natural selection in human populations has important applications to human history and medicine. Positive natural selection drives the increase in beneficial alleles and plays a role in explaining diversity across human populations. By discovering traits subject to positive selection, we can better understand the population level response to environmental pressures including infectious disease. Our study examines unusual population differentiation between three large data sets to detect natural selection. The populations examined, African Americans, Nigerians, and Gambians, are genetically close to one another (F ST < 0.01 for all pairs), allowing us to detect selection even with moderate changes in allele frequency. We also develop a tree-based method to pinpoint the population in which selection occurred, incorporating information across populations. Our genome-wide significant results corroborate loci previously reported to be under selection in Africans including HBB and CD36. At the HLA locus on chromosome 6, results suggest the existence of multiple, independent targets of population-specific selective pressure. In addition, we report a genome-wide significant (p = 1.36 × 10 -11) signal of selection in the prostate stem cell antigen (PSCA) gene. The most significantly differentiated marker in our analysis, rs2920283, is highly differentiated in both Africa and East Asia and has prior genome-wide significant associations to bladder and gastric cancers. © 2011 The American Society of Human Genetics.

Coordinated Regulation By Agra, Sara, And Sarr To Control Agr Expression In Staphylococcus Aureus, Dindo Reyes, Diego O. Andrey, Antoinette Monod, William L. Kelley, Gongyi Zhang, Ambrose L. Cheung

Dartmouth Scholarship

The agr locus of Staphylococcus aureus is composed of two divergent transcripts (RNAII and RNAIII) driven by the P2 and P3 promoters. The P2-P3 intergenic region comprises the SarA/SarR binding sites and the four AgrA boxes to which AgrA binds. We reported here the role of AgrA, SarA, and SarR on agr P2 and P3 transcription. Using real-time reverse transcription (RT)-PCR and promoter fusion studies with selected single, double, triple, and complemented mutants, we showed that AgrA is indispensable to agr P2 and P3 transcription, whereas SarA activates and SarR represses P2 transcription. In vitro runoff transcription assays revealed that …

Bioluminescent Imaging Reveals Divergent Viral Pathogenesis In Two Strains Of Stat1-Deficient Mice, And In Αßγ Interferon Receptor-Deficient Mice, Tracy Jo Pasieka, Lynne Collins, Megan A. O'Connor, Yufei Chen, Zachary M. Parker, Brent L. Berwin, David R. Piwnica-Worms, David A. Leib

Dartmouth Scholarship

Pivotal components of the IFN response to virus infection include the IFN receptors (IFNR), and the downstream factorsignal transducer and activator of transcription 1 (Stat1). Mice deficient for Stat1 and IFNR (Stat12/2 and IFNaßcR2/2 mice) lack responsiveness to IFN and exhibit high sensitivity to various pathogens. Here we examined herpes simplex virus type 1 (HSV-1) pathogenesis in Stat12/2 mice and in IFNaßcR2/2 mice following corneal infection and bioluminescent imaging. Two divergent and paradoxical patterns of infection were observed. Mice with an N-terminal deletion in Stat1 (129Stat12/2(N-term)) had transient infection of the liver and spleen, but succumbed to encephalitis by day …

Current, September 06, 2011, University Of Missouri-St. Louis

Current (2010s)

No abstract provided.

Infectious Cdna Clone Of The Modified Live Virus Vaccine Strain Of Equine Arteritis Virus, Udeni B. R. Balasuriya, Peter J. Timoney, Jianquan Wang

Veterinary Science Faculty Patents

An isolated polynucleotide molecule includes a DNA sequence encoding an infectious RNA molecule encoding a modified live viral strain of an Equine arteritis virus, wherein the DNA sequence is SEQ ID NO:1 or a degenerate variant thereof. Also provided are transformed or transfected host cells including that sequence, vectors including the sequence, and isolated infectious RNA molecules encoded by the sequence. Further, a modified DNA sequence encoding an infectious RNA molecule encoding a modified live viral strain of an Equine arteritis virus is provided wherein the DNA sequence is SEQ ID NO:2 or a degenerate variant thereof, including a silent …

Trends Of Increase In Western Medical Services In Traditional Medicine Hospitals In China, Jay J. Shen, Ying Wang, Fang Lin, Jun Lu, Charles B. Moseley, Mei Sun, Mo Hao

School of Public Health Faculty Research

Background:

Compare changes in types of hospital service revenues between traditional Chinese medicine (TCM) hospitals and Western-medicine based general hospitals.

Methods:

97 TCM hospitals and 103 general hospitals were surveyed in years of 2000 and 2004. Six types of medical service revenue between the two types of hospitals were compared overtime. The national statistics from 1999 to 2008 were also used as complementary evidence.

Results:

For TCM hospitals, the percentage of service revenue from Western medicine increased from 44.3% to 47.4% while the percentage of service revenue from TCM declined from 26.4% to 18.8% from 1999 to 2004. Percentages of …

Bjc Today

BJC Today

No abstract provided.

Differences Between Trypanosoma Brucei Gambiense Groups 1 And 2 In Their Resistance To Killing By Trypanolytic Factor 1, Paul Capewell, Nicola J. Veitch, C. Michael R. Turner, Jayne Raper, Matthew Berriman, Stephen L. Hajduk, Annette Macleod

Publications and Research

Background: The three sub-species of Trypanosoma brucei are important pathogens of sub-Saharan Africa. T. b. brucei is unable to infect humans due to sensitivity to trypanosome lytic factors (TLF) 1 and 2 found in human serum. T. b. rhodesiense and T. b. gambiense are able to resist lysis by TLF. There are two distinct sub-groups of T. b. gambiense that differ genetically and by human serum resistance phenotypes. Group 1 T. b. gambiense have an invariant phenotype whereas group 2 show variable resistance. Previous data indicated that group 1 T. b. gambiense are resistant to TLF-1 due in-part to reduced …

Effect Of Lactobacilli Sources On Escherichia Coli And Staphylococcus Aureus Adherence To Uroepithelial Cells, Harith J. F. Al-Mathkhury, Alaa' Mohammed Hasan

Baghdad Science Journal

The inhibitory action of four lactobacilli isolates Lactobacillus bulgaricus, L. acidophilus, L. plantarum and L. fermentum, isolated from four different samples; yoghurt, vinegar, saliva and vagina respectively, on Escherichia coli and Staphylococcus aureus adhesion to uroepithelial cells were investigated. Results showed that all Lactobacillus isolates or their supernatant were able to reduce the number of the uropathogens attached to uroepithelial cells. However, inhibition level of lactobacilli cells was higher than their supernatant. Nevertheless, the human indigenous lactobacilli (L. fermentum and L. plantarum) were more competitive than food lactobacilli (L. acidophilus and L. bulgaricus).

Tropical Medicine: A Clinical Text, 8th Edition, Revised And Expanded, Kevin M. Cahill, M.D.

Institute of International Humanitarian Affairs

The history of tropical medicine is as dramatic as the story of humankind. It has its own myths and legends, including tales of epidemics that destroyed whole civilizations. Today, with silent stealth, tropical diseases still claim more lives than all the current wars combined. Having had the privilege of working throughout Africa, Asia, and Latin America, as well as in the great medical centers of Europe and the United States, the author presents the details essential for understanding pathogenesis, clinical manifestations, therapy, and prevention of the major tropical diseases. The text, now in its eighth edition, has been used for …

Gene Expression In The Right Ventricle During Development Of Pulmonary Hypertension, Jennifer Drake

Theses and Dissertations

Pulmonary arterial hypertension (PAH) is a disease of the lung vessels that causes severe effects on the right ventricle of the heart; ultimately, most patients with severe PAH die as a result of right heart failure. However, little is known about the causes of right heart failure. Here, we describe a pattern of gene expression that differs between the normal rat left ventricle (LV) and right ventricle (RV). These genes are known to be involved in the development of the heart as well as adaptations to the heart during stress. This gene expression pattern is used as a baseline to …

Colleague, Fall 2011, University Of Arkansas, Fayetteville. College Of Education And Health Professions, University Of Arkansas, Fayetteville. Department Of Education Reform

Colleague

No abstract provided.

An Excursion Into The Intriguing World Of Polymeric Tl(I) And Ag(I) Cyanoximates, Nikolay Gerasimchuk

College of Natural and Applied Sciences

The reaction of hot (~95°C) aqueous solutions of Tl2CO3 with solid HL (HL = NC-C(=N-OH)-R is a cyanoxime, and R is an electron-withdrawing group; 37 ligands are known up-to-date) leads to crystalline yellow/orange TlL. Similarly, the reaction between AgNO3 and ML (M = K+, Na+; L = anion of the monodeprotonated cyanoxime) this time at room temperature in mixed ethanol/aqueous solutions leads to sparingly soluble, colored AgL in high-yield. All synthesized monovalent Tl and Ag complexes were characterized using a variety of spectroscopic methods and X-ray analysis, which revealed the formation of primarily 2D coordination polymers of different complexity. In …

Checkup, Lehigh Valley Health Network

Checkup

No abstract provided.

Progress Notes, Lehigh Valley Health Network

Progress Notes

No abstract provided.

Getting Noticed: Middle Childhood In Cross-Cultural Perspective, David F. Lancy, M. Annette Grove

Sociology, Social Work and Anthropology Faculty Publications

Although rarely named, the majority of societies in the ethnographic record demarcate a period between early childhood and adolescence. Prominent signs of demarcation are: for the first time, pronounced gender separation in fact and in role definition; increased freedom of movement for boys while girls may be bound more tightly to their mothers; and heightened expectations for socially responsible behavior. But, above all, middle childhood is about coming out of the shadows of community life and assuming a distinct, lifetime character. Naming and other rites of passage sometimes acknowledge this transition, but it is, reliably, marked by the assumption or …

A New Undergraduate Curriculum On Mathematical Biology At University Of Dayton, Muhammad Usman, Amit Singh

Mathematics Faculty Publications

The beginning of modern science is marked by efforts of pioneers to understand the natural world using a quantitative approach. As Galileo wrote, "the book of nature is written in the language of mathematics". The traditional undergraduate course curriculum is heavily focused on individual disciplines like biology, physics, chemistry, mathematics rather than interdisciplinary courses. This fragmented teaching of sciences in majority of universities leave biology outside the quantitative and mathematical approaches. The landscape of biomedical science has transformed dramatically with advances in high throughput experimental approaches, which led to the huge amount of data. The best possible approach to generate …

Meow ... Cat Chow!

Animal Sheltering 2010-12

Feeding kitties in the shelter

Not Just Toolin’ Around

Animal Sheltering 2010-12

Online assessment helps shelters combat infectious disease

Microbial Pathogens And Biotoxins: State Of The Gulf Of Maine Report, Steve Jones

PREP Reports & Publications

No abstract provided.

When Push Comes To Shove: Mandatory Immunization In Times Of Pandemic-Type Emergencies, Philip P. Houle, Suzanne R. Houle

Buffalo Public Interest Law Journal

No abstract provided.

Benchmarks 2011, September 9, The Rockefeller University

BenchMarks 2011

BenchMarks. Friday, September 9, 2011

The community newsletter of The Rockefeller University

BenchMarks is published monthly and is distributed on the campus of The Rockefeller University. It produced by the Office of Communications and Public Affairs

Law School Record, Vol. 58, No. 1 (Fall 2011), Law School Record Editors

Law School Record

• Law and Economics 2.0: Chicago’s New Law and Economics Initiative
• Chicago Law and Economics: A History
• The Future of Law and Economics
• University of Chicago Law School Graduation 2011

Enhancing Genome Investigations In The Mosquito Culex Quinquefasciatus Via Bac Library Construction And Characterization, Paul V. Hickner, Becky Debruyn, Diane D. Lovin, Akio Mori, Christopher Saski, David W. Severson

Publications

Background

Culex quinquefasciatus (Say) is a major species in the Culex pipiens complex and an important vector for several human pathogens including West Nile virus and parasitic filarial nematodes causing lymphatic filariasis. It is common throughout tropical and subtropical regions and is among the most geographically widespread mosquito species. Although the complete genome sequence is now available, additional genomic tools are needed to improve the sequence assembly.

Findings

We constructed a bacterial artificial chromosome (BAC) library using the pIndigoBAC536 vector and HindIII partially digested DNA isolated from Cx. quinquefasciatus pupae, Johannesburg strain (NDJ). Insert size was estimated by Not …