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Thermal Tolerance, Physiologic Condition, And Population Genetics Of Dreissenid Mussels (Dreissena Polymorpha And D. Rostriformis Bugensis) Relative To Their Invasion Of Waters In The Western United States, John T. Morse

Biology Dissertations - Archive

Zebra mussels, Dreissena polymorpha, and quagga mussels, D. rostriformis bugensis, Eurasian freshwater bivalves, invaded the North American (NA) Great Lakes in the mid 1980's. Subsequently, they invaded water bodies east of the Rocky Mountains. Their more recent expansion into warm, isolated, southwestern U.S. water bodies suggests that both species are developing thermally-tolerant physiological races. Chronic upper thermal tolerance limits were determined for D. polymorpha populations from Winfield City Lake (KS) (WCL), Lake Oologah (OK) (OL) and Hedges Lake (NY) (HL), and the D. rostriformis bugensis population from Lake Mead (NV/AZ) (LM). Individuals from WCL had 12-d and 28-d incipient upper …

Sex, War And Disease: The Effects Of Infection On Horn Size And Intra-Sexual Competition In The Broad-Horned Flour Beetle, Gnathocerus Cornutus, Amrita Naidu

Biology Theses - Archive

Sexual selection is widely used to explain the evolution of mating systems where most often it is manifest as the competition among males for access to females. This competition takes the form of direct male-male interactions in broad-horned flour beetle Gnathocerus cornutus and often results in exaggerated male phenotypes. Sexually selected traits are interesting because in many cases their existence appears to contradict natural selection. For example, the "handicap hypothesis" suggests that there may be a trade-off between immune response and horn size because mounting an immune response necessary for survival (natural selection) may divert resources away from growing longer …

Determining The Phylogeographic Dynamics Of The Edge Relationships Between Aphonopelma Hentzi (Girard) And Its Neighbors Along The Colorado River Basin (Araneae, Mygalomorphae, Theraphosidae), Chris A. Hamilton

Biology Theses - Archive

Theraphosids, commonly known as “tarantulas”, are a group of large, hirsute spiders that have drawn relatively little interest in ecological, behavioral, or evolutionary research from biologists. The lack of research on theraphosids is problematic because of conservation concerns for this group and the need to compile good life history data, and determine taxonomic and phylogenetic relationships. I examined the phylogenetic relationships among populations of tarantulas of the genus Aphonopelma along the Colorado River basin in Texas to construct a framework within which to test ecological, evolutionary, and biogeographical hypotheses in an area of species transition and possible Pleistocene refugia. In …

The Mysterious Unfoldome: Structureless, Underappreciated, Yet Vital Part Of Any Given Proteome, Vladimir N. Uversky

Molecular Medicine Faculty Publications

Contrarily to the general believe, many biologically active proteins lack stable tertiary and/or secondary structure under physiological conditions in vitro. These intrinsically disordered proteins (IDPs) are highly abundant in nature and many of them are associated with various human diseases. The functional repertoire of IDPs complements the functions of ordered proteins. Since IDPs constitute a significant portion of any given proteome, they can be combined in an unfoldome; which is a portion of the proteome including all IDPs (also known as natively unfolded proteins, therefore, unfoldome), and describing their functions, structures, interactions, evolution, and so forth. Amino acid sequence and …

Protein Disorder In The Human Diseasome: Unfoldomics Of Human Genetic Diseases, Uros Midic, Christopher J. Oldfield, A. Keith Dunker, Zoran Obradovic, Vladimir N. Uversky

Molecular Medicine Faculty Publications

Background: Intrinsically disordered proteins lack stable structure under physiological conditions, yet carry out many crucial biological functions, especially functions associated with regulation, recognition, signaling and control. Recently, human genetic diseases and related genes were organized into a bipartite graph (Goh KI, Cusick ME, Valle D, Childs B, Vidal M, et al. (2007) The human disease network. Proc Natl Acad Sci U S A 104: 8685–8690). This diseasome network revealed several significant features such as the common genetic origin of many diseases.

Methods and findings: We analyzed the abundance of intrinsic disorder in these diseasome network proteins by means of several …

Influence Of Sequence Changes And Environment On Intrinsically Disordered Proteins, Amrita Mohan, Vladimir N. Uversky, Predrag Radivojac

Molecular Medicine Faculty Publications

Many large-scale studies on intrinsically disordered proteins are implicitly based on the structural models deposited in the Protein Data Bank. Yet, the static nature of deposited models supplies little insight into variation of protein structure and function under diverse cellular and environmental conditions. While the computational predictability of disordered regions provides practical evidence that disorder is an intrinsic property of proteins, the robustness of disordered regions to changes in sequence or environmental conditions has not been systematically studied. We analyzed intrinsically disordered regions in the same or similar proteins crystallized independently and studied their sensitivity to changes in protein sequence …

Protein Intrinsic Disorder And Influenza Virulence: The 1918 H1n1 And H5n1 Viruses, Gerard Kian-Meng Goh, A. Keith Dunker, Vladimir N. Uversky

Molecular Medicine Faculty Publications

Background: The 1918 H1N1 virus was a highly virulent strain that killed 20–50 million people. The cause of its virulence remains poorly understood.

Methods: Intrinsic disorder predictor PONDR® VLXT was used to compare various influenza subtypes and strains. Three-dimensional models using data from X-ray crystallographic studies annotated with disorder prediction were used to characterize the proteins.

Results: The protein of interest is hemagglutin (HA), which is a surface glycoprotein that plays a vital role in viral entry. Distinct differences between HA proteins of the virulent and non-virulent strains are seen, especially in the region near residues 68–79 of the HA2. …

Cover, Ornitología Neotropical

Ornitología Neotropical

No abstract provided.

Distribution, Abundance, And Survival Of Nesting American Dippers Near Juneau, Alaska, Mary F. Wilson, Grey W. Pendleton, Katherine M. Hocker

Western Birds

No abstract provided.

Advertisement, Western Birds

Western Birds

No abstract provided.

Accent On Learning, 2009-2010, University Of South Florida

USF Catalogs (Accent on Learning)

No abstract provided.

Viet. Assoc. For Victims Of Agent Orange V. Dow Chem. Co., 517 F.3d 104 (2d Cir. 2008) (Holding That District Court Lacked Jurisdiction Under The Alien Tort Statute To Consider The Claims Of A Vietnam Nonprofit Group And Vietnam Nationals For Injuries Allegedly Sustained From Exposure To Herbicides During The Vietnam War, That State Law Claims Were Barred By The Government Contractor Defense And The District Court Did Not Abuse Its Discretion When It Denied Extraterritorial Injunctive Relief), Cert. Denied, 555 U.S. 1218 (2009)., Roger J. Miner

Circuit Court Opinions

No abstract provided.

Primary Cilia: Highly Sophisticated Biological Sensors, Wissam A. Aboualaiwi, Shao T. Lo, Surya M. Nauli

Pharmacy Faculty Articles and Research

Primary cilia, thin hair-like structures protruding from the apical surface of most mammalian cells, have gained the attention of many researchers over the past decade. Primary cilia are microtubule-filled sensory organelles that are enclosed within the ciliary membrane. They originate at the cell surface from the mother centriole that becomes the mature basal body. In this review, we will discuss recent literatures on the roles of cilia as sophisticated sensory organelles. With particular emphasis on vascular endothelia and renal epithelia, the mechanosensory role of cilia in sensing fluid shear stress will be discussed. Also highlighted is the ciliary involvement in …

Assessment Of Biology Majors’ Versus Nonmajors’ Views On Evolution, Creationism, And Intelligent Design, Guillermo Paz-Y-Mino C., Avelina Espinosa

Arts & Sciences Faculty Publications

The controversy around evolution, creationism, and intelligent design resides in a historical struggle between scientific knowledge and popular belief. Four hundred seventy-six students (biology majors n=237, nonmajors n=239) at a secular liberal arts private university in Northeastern United States responded to a five-question survey to assess their views about: (1) evolution, creationism, and intelligent design in the science class; (2) students’ attitudes toward evolution; (3) students’ position about the teaching of human evolution; (4) evolution in science exams; and (5) students’ willingness to discuss evolution openly. There were 60.6% of biology majors and 42% of nonmajors supported the exclusive teaching …

Concerning The Disposition Of Evidence Collected In Criminal Cases., Colorado General Assembly

Session Laws 2001-Present

No abstract provided.

Concerning Sales And Use Tax Refunds For Certain Colorado-Based Technology Companies., Colorado General Assembly

Session Laws 2001-Present

No abstract provided.

Concerning The Continuation Of The Certification Of Nurse Aides By The State Board Of Nursing, And Making An Appropriation Therefor., Colorado General Assembly

Session Laws 2001-Present

No abstract provided.

The Mckillop Library Waste Stream, Kalyna Macko, Kelsy Patnaude, Other Students

BIO140 Carbon Footprint Project

The McKillop Library's resources come at an environmental cost because more resources are being used than is realized. The focus is on the amount of carbon emissions and waste that is being produced by the library. Carbon emissions are the leading cause of climate change, and through recycling the library can lower the amount of carbon the library is producing. If recycling and re-using materials becames more of a common procedure in library, there is little doubt that it would be more environmentally friendly.

Effects Of Temperature On Reproduction And Survival Of The Calanoid Copepod Pseudodiaptomus Pelagicus, Andrew L. Rhyne, Cortney L. Ohs, Erik Stenn

Arts & Sciences Faculty Publications

Four experiments were conducted on the calanoid copepod, Pseudodiaptomus pelagicus, to determine the effects of temperature (24, 26, 28, 30, 32, and 34 °C) on survival, development time, reproductive output, and population growth in order to define the optimal temperature for culture. The first experiment stocked early stage nauplii into 1 L beakers and cultured them using standard procedures until five days after the first mature adults were observed; from this survival, sex ratio, time to maturation, and fecundity were measured. The second and third experiments evaluated the effects of temperature on nauplii production by stocking individual pairs and …

The Development Of Jaw Motion For Mastication, Erin M. Wilson, Jordan R. Green

Department of Special Education and Communication Disorders: Faculty Publications

The developmental course of early chewing has rarely been studied, although such knowledge is essential for understanding childhood feeding and swallowing disorders. The goal of this investigation was to quantitatively describe age- and consistency-related changes in jaw kinematics during early chewing development. An optical-motion tracking system was used to record jaw movements during chewing in 3-dimensions in 11 typically-developing participants longitudinally from 9–30 months of age. Age related changes in jaw movement were described for both puree and regular consistencies. The findings demonstrated that the development of rotary jaw motion, jaw motion speed, and management of consistency upgrades are protracted …

In Situ Manipulation Of Vertically Migrating Gelatinous Zooplankton Using Nighttime Blue-Water Scuba In The South-Central Adriatic Sea, William M. Graham, John H. Costello, Sean Colin, Alenka Malej, Davor Lučić, Vladimir Onofri, Adam Benović

Arts & Sciences Faculty Publications

Technological advance in undersea exploration (e.g. tethered cameras, remotely operated vehicles [ROVs], Autonomous Underwater Vehicles [AUVsG, and manned submersibles)have opened new windows into diversityand distribution of fragile gelatinous organisms in the vast mesopelagic realm(300 m-1000 m deep). While exstraordinary in expanding our view of its richness, mesopelagic exploration remains largely a "look but don't touch" environment and this limits of our ability to understand these animals through physical manipulation relevant to the finer scales of the individual organism. We have been conducting a series of in situ observations and manipulations using blue-water SCUBA during the night at a 1, 200 …

A Nonsense Mutation In A Cinnamyl Alcohol Dehydrogenase Gene Is Responsible For The Sorghum Brown Midrib6 Phenotype1[W][Oa], Scott E. Sattler, Aaron J. Saathoff, Eric J. Haas, Nathan A. Palmer, Deanna L. Funnell, Gautam Sarath, Jeffrey F. Pedersen

Department of Agronomy and Horticulture: Faculty Publications

Recently, bmr6 plants were shown to have limited cinnamyl alcohol dehydrogenase activity (CAD; EC 1.1.1.195), the enzyme that catalyzes the conversion of hydroxycinnamoyl aldehydes (monolignals) to monolignols. A candidate gene approach was taken to identify Bmr6. Two CAD genes (Sb02g024190 and Sb04g005950) were identified in the sorghum genome based on similarity to known CAD genes and through DNA sequencing a nonsense mutation was discovered in Sb04g005950 that results in a truncated protein lacking the NADPH-binding and C-terminal catalytic domains. Immunoblotting confirmed that the Bmr6 protein was absent in protein extracts from bmr6 plants. Phylogenetic analysis indicated that Bmr6 is …

X4 Human Immunodeficiency Virus Type 1 Gp120 Down-Modulates Expression And Immunogenicity Of Codelivered Antigens, Avi-Hai Hovav, Michael Santosuosso, Maytal Bivas-Benita, Andre Plair, Alex Cheng, Mazal Elnekave, Elda Righi, Tao Chen, Satoshi Kashiwagi, Michael W. Panas, Shi-Hua Xiang, Karina Furmanov, Norman L. Letvin, Mark C. Poznansky

Nebraska Center for Virology: Faculty Publications

In order to increase the immune breadth of human immunodeficiency virus (HIV) vaccines, strategies such

as immunization with several HIV antigens or centralized immunogens have been examined. HIV-1 gp120

protein is a major immunogen of HIV and has been routinely considered for inclusion in both present and

future AIDS vaccines. However, recent studies proposed that gp120 interferes with the generation of immune

response to codelivered antigens. Here, we investigate whether coimmunization with plasmid-encoded gp120

alters the immune response to other coadministered plasmid encoded antigens such as luciferase or ovalbumin

in a mouse model. We found that the presence of gp120 …

Identification And Characterization Of A New Kaposi’S Sarcoma-Associated Herpesvirus Replication And Transcription Activator (Rta)- Responsive Element Involved In Rta-Mediated Transactivation, Hui-Ju Wen, Veenu Minhas, Charles Wood

Nebraska Center for Virology: Faculty Publications

Kaposi’s sarcoma-associated herpesvirus (KSHV) replication and transcription activator (RTA) is well established as a key transcriptional activator that regulates the KSHV life cycle from latency to lytic replication. It is expressed immediately after infection and activates a number of viral genes leading to virus replication. The RTA-responsive element (RRE) in the RTA target gene promoters is critical for RTA to mediate this transactivation. A number of non-conserved RREs have been identified in various RTA-responsive promoters, and AT-rich sequences have been proposed to serve as RTA targets, but no consensus RRE sequence has been identified so far. Two nonconserved RREs (RRE1 …

Use Of Average Mutual Information For Studying Changes In Hiv Populations, Khalid Sayood, Federico Hoffman, Charles Wood

Nebraska Center for Virology: Faculty Publications

Average mutual information (AMI) has been used in a number of applications in bioinformatics. In this paper we present its use to study genetic changes in populations; in particular populations of HIV viruses. Disease progression of HIV-1 infection in infants can be rapid resulting in death within the the first year, or slow, allowing the infant to survive beyond the first year. We study the development of rapid and slow progressing HIV population using AMI charts based on average mutual information among amino acids in the env gene from a population of 1142 clones derived from seven infants with slow …

Comparison Of Replication-Competent, First Generation, And Helper-Dependent Adenoviral Vaccines, Eric A. Weaver, Pramod N. Nehete, Stephanie S. Buchl, Julien S. Senac, Donna Palmer, Philip Ng, K. Jagannadha Sastry, Michael A. Barry

Nebraska Center for Virology: Faculty Publications

All studies using human serotype 5 Adenovirus (Ad) vectors must address two major obstacles: safety and the presence of pre-existing neutralizing antibodies. Helper-Dependent (HD) Ads have been proposed as alternative vectors for gene therapy and vaccine development because they have an improved safety profile. To evaluate the potential of HD-Ad vaccines, we compared replication-competent (RC), first-generation (FG) and HD vectors for their ability to induce immune responses in mice. We show that RC-Ad5 and HD-Ad5 vectors generate stronger immune responses than FG-Ad5 vectors. HD-Ad5 vectors gave lower side effects than RC or FG-Ad, producing lower levels of tissue damage and …

Decent Society Index: A Research Note, Judith, Jenniffer Santos, Chelsea Sessoms

Societies Without Borders

People everywhere are discovering that their own views about a decent society are widely shared around the world. The Universal Declaration of Human Rights already made this clear in 1948 by referring to “a common understanding” of rights and freedoms and a “recognition of the inherent dignity and of the equal and inalienable rights of all members of the human family.” A challenge for social scientists is to develop dimensions for an index that measures variation in the extent to which societies are decent. Such an index would be useful in research for comparative and analytical purposes, and it could …

Immigrants' Rights In The Public Sphere: Hannah Arendt's Concepts Reconsidered, San Martín

Societies Without Borders

Based on Arendt's concepts of public and private spheres, immigration issues can be approached from an emphasis on how the most fundamental of all human rights, which is being denied to immigrants, is the most basic constituent of the human condition: the ability to interact in the public realm through action and speech. The granting of this right would enable immigrants to become unique human beings, with the capacity for transformation. As they are presently deprived of these and other rights, they are confined to the most primitive sphere, that is, the one of pure survival. Therefore, a differentiation must …

W.E.B. Du Bois, Race, And Human Rights, Elias

Societies Without Borders

Du Bois' sociological thought reveals an overlooked tension in the pursuit of human rights, a tension between universal human rights for all people, in general, and a race-specific human rights agenda for blacks, in particular. Du Bois recognizes both universal human rights for all individuals, groups, nations, and international bodies (regardless of race, gender, class, and other human divisions) and case-specific human rights for particular individuals, groups, nations, and international bodies (with regard to race, gender, class, and other human divisions). I aim to demonstrate why Du Bois' acknowledgment of universal and case-specific human rights, and dynamics of their interrelationship, …

Jihad, Race And Western Media, Post-September 11, Tagg

Societies Without Borders

The article examines jihad within what is loosely referred to as “the Western imagination.” Through the analysis of both popular stereotypes and the broader historical context of religious antagonism and (neo)colonialism, the paper discusses both contemporary political rhetoric and a pair of liberal post September-11 political cartoons. While European and North American political leaders have often stereotyped Muslims in order to justify various “axis of evil” bandwagons and further political and economic interests throughout the Islamic Crescent, I argue here that the liberal media's critiques also often rely on fallacious embodied stereotypes. The pervasiveness of such representations has important implications …