Digital Commons Network^™

First Report Of Soybean Rust Caused By Phakopsora Pachyrhizi In Nebraska, S. R. Watson, L. J. Giesler, A. D. Ziems, T. E. Brovont

Department of Plant Pathology: Faculty Publications

Soybean rust caused by Phakopsora pachyrhizi Sydow was first observed in the continental United States in Louisiana in November 2004 (2). As part of the national soybean rust monitoring effort, samples were collected on 3 October 2007 during the scouting of fields with green leaves in southeastern Nebraska. After incubation at room temperature for 24 h, uredinea and urediniospores were observed with microscopic examination. Urediniospores were obovoid, hyaline to pale brown, and measured 20 to 30 × 18 to 20 μm.

Technical Variables In High-Throughput Mirna Expression Profiling: Much Work Remains To Be Done, Peter T. Nelson, Wang-Xia Wang, Bernard R. Wilfred, Guiliang Tang

Pathology and Laboratory Medicine Faculty Publications

MicroRNA (miRNA) gene expression profiling has provided important insights into plant and animal biology. However, there has not been ample published work about pitfalls associated with technical parameters in miRNA gene expression profiling. One source of pertinent information about technical variables in gene expression profiling is the separate and more well-established literature regarding mRNA expression profiling. However, many aspects of miRNA biochemistry are unique. For example, the cellular processing and compartmentation of miRNAs, the differential stability of specific miRNAs, and aspects of global miRNA expression regulation require specific consideration. Additional possible sources of systematic bias in miRNA expression studies include …

Nitric Oxide-Induced Bacteriostasis And Modification Of Iron-Sulphur Proteins In Escherichia Coli, Binbin Ren, Nianhui Zhang, Juanjuan Yang, Huangen Ding

Faculty Publications

The nitric oxide (NO) cytotoxicity has been well documented in bacteria and mammalian cells. However, the underlying mechanism is still not fully understood. Here we report that transient NO exposure effectively inhibits cell growth of Escherichia coli in minimal medium under anaerobic growth conditions and that cell growth is restored when the NO-exposed cells are either supplemented with the branched-chain amino acids (BCAA) anaerobically or returned to aerobic growth conditions. The enzyme activity measurements show that dihydroxyacid dehydratase (IlvD), an iron-sulphur enzyme essential for the BCAA biosynthesis, is completely inactivated in cells by NO with the concomitant formation of the …

Identification Of A Functionally Essential Amino Acid For Arabidopsis Cyclic Nucleotide Gated Ion Channels Using The Chimeric Atcngc11/12 Gene, Joyce Baxter, Wolfgang Moeder, William Urquhart, Dea Shahinas, Kimberley Chin, Dinesh Christendat, Hong Gu Kang, Magdalena Angelova, Naohiro Kato, Keiko Yoshioka

Faculty Publications

We used the chimeric Arabidopsis cyclic nucleotide-gated ion channel AtCNGC11/12 to conduct a structure-function study of plant cyclic nucleotide-gated ion channels (CNGCs). AtCNGC11/12 induces multiple pathogen resistance responses in the Arabidopsis mutant constitutive expresser of PR genes 22 (cpr22). A genetic screen for mutants that suppress cpr22-conferred phenotypes identified an intragenic mutant, #73, which has a glutamate to lysine substitution (E519K) at the beginning of the eighth β-sheet of the cyclic nucleotide-binding domain in AtCNGC11/12. The #73 mutant is morphologically identical to wild-type plants and has lost cpr22-related phenotypes including spontaneous cell death and enhanced pathogen resistance. Heterologous expression analysis …

Transcriptional Profiling Of Mature Arabidopsis Trichomes Reveals That Noeck Encodes The Mixta-Like Transcriptional Regulator Myb106, Marc J. Jakoby, Doris Falkenhan, Michael T. Mader, Ginger Brininstool, Elisabeth Wischnitzki, Nicole Platz, Andrew Hudson, Martin Hülskamp, John Larkin, Arp Schnittger

Faculty Publications

Leaf hairs (trichomes) of Arabidopsis (Arabidopsis thaliana) have been extensively used as a model to address general questions in cell and developmental biology. Here, we lay the foundation for a systems-level understanding of the biology of this model cell type by performing genome-wide gene expression analyses. We have identified 3,231 genes that are up-regulated in mature trichomes relative to leaves without trichomes, and we compared wild-type trichomes with two mutants, glabra3 and triptychon, that affect trichome morphology and physiology in contrasting ways. We found that cell wall-related transcripts were particularly overrepresented in trichomes, consistent with their highly elaborated structure. In …

Victorian Organ, Christine Dunn

Theses

This paper is a companion piece to my short film, “Victorian Organ”. This film is an effort to contextualize the mechanical, or man-made, within nature. It is an assertion that there really is no "man vs. nature"- (a radically illogical and inherently self-destructive notion), but only man as nature, in which case, alienation and disharmony ultimately lies in our own self-perception. The impulse to identify a relationship between the mechanical and organic is inspired not only by my observations of our human society, but as a way to decode my own inner workings –an urge to reconcile seemingly major dichotomies …

Regulation Of The Mouse Treacher Collins Syndrome Homolog (Tcof1) Promoter Through Differential Repression Of Constitutive Expression, Kathryn H. Shows, Rita Shiang

Biology & Environmental Sciences Faculty Publications

Treacher Collins syndrome is an autosomal-dominant mandibulofacial dysostosis caused by haploinsufficiency of the TCOF1 gene product treacle. Mouse Tcof1 protein is approximately 61% identical and 71% similar to treacle, and heterozygous knockout of Tcof1 causes craniofacial malformation. Tcof1 expression is high in developing neural crest, but much lower in other tissues. To investigate this dual regulation, highly conserved regions upstream of TCOF1 homologs were tested through deletion and mutation reporter assays, and conserved predicted transcription factor binding sites were assessed through chromatin binding studies. Assays were performed in mouse P19 embryonic carcinoma cells and in HEK293 cells to determine differential …

Memory Functioning In Children With Reading Disabilities And/Or Attention Deficit/Hyperactivity Disorder: A Clinical Investigation Of Their Working Memory And Long-Term Memory Functioning., Michelle Y. Kibby, Morris J. Cohen

Publications

We examined memory functioning in children with reading disabilities (RD), Attention deficit/hyperactivity disorder (ADHD), and RD/ADHD using a clinic sample with a clinical instrument: the Children's Memory Scale, enhancing its generalizability. Participants included 23 children with RD, 30 with ADHD, 30 with RD/ADHD, and 30 controls. Children with RD presented with reduced verbal short-term memory (STM) but intact visual STM, central executive (CE), and long-term memory (LTM) functioning. Their deficit in STM appeared specific to tasks requiring phonetic coding of material. Children with ADHD displayed intact CE and LTM functioning but reduced visual-spatial STM, especially when off stimulant medication. Children …

Poem No. 100, November 2008, Huntsville Literary Association (Huntsvile, Ala.)

POEM

The Huntsville Literary Association was founded in 1967 to provide a community for writers, readers, and artists through workshops, readings, and educational programs. One of their key contributions is the literary magazine titled POEM which showcases original writings from both established and emerging writers. This issue features poems from various writers.

Central Blockade Of Oxytocin Receptors During Mid-Late Gestation Reduces Amplitude Of Slow Afterhyperpolarization In Supraoptic Oxytocin Neurons, R. Teruyama, D. L. Lipschitz, L. Wang, G. R. Ramoz, W. R. Crowley, S. L. Bealer, W. E. Armstrong

Faculty Publications

The neurohypophysial hormone oxytocin (OT), synthesized in magnocellular paraventricular (PVN) and supraoptic (SON) nuclei, is well known for its effects in lactation. Our previous studies showed that central OT receptor (OTR) binding is increased during gestation and that blockade of central OTRs, specifically during mid-late gestation, causes a delay in OT release during suckling and reduces weight gain in pups, suggesting decreased milk delivery. In the present study, we tested whether central OTR blockade during late gestation disrupts the gestation-related plasticity in intrinsic membrane properties. Whole cell current-clamp recordings were performed in OT neurons from pregnant rats (19-22 days in …

Synthesis And Spectroelectrochemistry Of N-Cobaltacarborane Porphyrin Conjugates, Erhong Hao, Min Zhang, E. Wenbo, Karl M. Kadish, Frank R. Fronczek, Brandy H. Courtney, M. Graça H. Vicente

Faculty Publications

N-Substituted porphyrins are well-known for the distortion they exhibit of the porphyrin plane through the sp3 hybridization of one of the pyrrolenic units. They have served as model compounds in investigations of many biochemical processes. In this paper, we developed an efficient route to N-substituted porphyrins, and report the synthesis of a series of new N-substituted cobaltacarborane - porphyrins containing one or two cobaltabisdicarbollide anions linked by (CH2CH2O) 2 chains to either the core porphyrin nitrogens or to a meso-aminophenyl group. These conjugates show different degrees of distortion of the porphyrin macrocycle, which affect their spectroscopic and electrochemical properties. In …

Effects Of M-Tyrosine On Feeding And Survival Of Formosan Subterranean Termites (Isoptera: Rhinotermitidae), Bal K. Gautam, Gregg Henderson

Faculty Publications

Amino acids are important to all animal life. Termites rely mainly on microorganisms to retrieve these nitrogen sources from their food. One naturally occurring amino acid, m-tyrosine, has been reported to be toxic to bacteria and weeds. Its direct effects on termite survival are unknown however. Both no-choice and choice bioassays using five different doses of m-tyrosine were conducted to determine the feeding response and survival of Formosan subterranean termites, Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae), in the laboratory. In no-choice tests, although all doses of m-tyrosine tested reduced filter paper consumption, only the highest dose tested (0.05 M) had significantly …

The Link, Vol 2, No. 11, November 2008, Illinois State University, Center For Integrated Professional Development

The Link

Newsletter for the Illinois State University Center for Integrated Professional Development

Deconstructing Masculinity In A ‘Female Bastion’: Ambiguities, Contradictions And Insights, Charles C. Fonchingong

Journal of International Women's Studies

This article is informed by my experiences teaching women’s studies and specifically feminist theory to predominantly female and male students offering Women’s studies. As a mainstream academic discipline at the University of Buea, housing the only such Department in Cameroon’s Higher Education system, this study uncovers the broader polemics regarding gender and women’s studies.

Against the backdrop of a patriarchal society, this study attempts to account for the shifting strands on masculinity and femininity and gender transgressions as played out by students taking women’s studies. It also analyses the notions, misconceptions and stereotypes that characterise the discipline of women’s studies, …

Film Review: Judith Butler: Philosophical Encounters Of The Third Kind, Cierra Olivia Thomas-Williams

Journal of International Women's Studies

Review of Judith Butler: Philosophical Encounters of the Third Kind by Paule Zadjermann (filmmaker)

Caveolin-1, Tgf-Β Receptor Internalization, And The Pathogenesis Of Systemic Sclerosis, Francesco Del Galdo, Michael P. Lisanti, Sergio A. Jimenez

Jefferson Institute of Molecular Medicine Papers and Presentations

PURPOSE OF REVIEW: To review the scientific literature supporting the participation of caveolin-1 in the pathogenesis of tissue fibrosis and the notion that modulation of the caveolin-1 pathway may represent a novel treatment for systemic sclerosis and other fibrotic diseases.

RECENT FINDINGS: Caveolin-1 plays an important role in the regulation of transforming growth factor-beta (TGF-beta) signaling owing to its participation in TGF-beta receptor internalization. TGF-beta receptor internalized through caveolin-1 lipid rafts undergoes rapid degradation, effectively decreasing TGF-beta signaling. Studies have shown that caveolin-1 knockdown in vitro markedly increased collagen gene expression in normal human lung fibroblasts. Caveolin-1 was reduced in …

Vol. 6, No. 05 (November/December 2008)

Indiana Law Update

No abstract provided.

Clinical Aspects Of Melatonin, Russel J. Reiter, Ahmet Korkmaz

Saudi Medical Journal

Melatonin is produced in the human pineal gland, particularly at night, with the circadian rhythm of blood melatonin levels closely paralleling its production within the pineal gland. Light exposure at night, or rapid transmeridian travel severely compromises the circadian production of melatonin. The disturbed melatonin rhythm contributes to jet lag and sleep inefficiency, both of which are improved by melatonin administration. Melatonin is also a highly effective direct free radical scavenger and antioxidant. In this capacity, melatonin reduces experimental cataractogenesis, traumatic injury to the spinal cord and brain, and protects against oxidative damage to neurons and glia in models of …

Antifeedant Activity Of Leaf Aqueous Extracts Of Selected Medicinal Plants On Vi Instar Larva Of Helicoverpa Armigera (Hübner), S. Ramya Et Al.

Ethnobotanical Leaflets

Phytochemicals, especially botanical insecticides are currently of interest because of their successful application in plant protection as biocontrol agents. Biological activity of leaf aqueous extract of ten selected medicinal plants were evaluated against the fourth-instar larvae of gram pod borer Helicoverpa armigera (Hübner), (Lepidoptera: Noctuidae). Antifeedant activity of leaf aqueous extracts of Abutilon indicum L., Achyranthus aspera L., Aerva lanata L., Albiziz amara (Roxb), Andrographis paniculata Ness., Cardiospermum halicacabum L., Cassia tora L., Catharanthus roseus L (G) Don., Datura metal L. and Tribulus terrestris L. were evaluated in this study. Preliminary screening after 24 h of exposure with leaf aqueous …

Studies On Antimicrobial Efficiency Of Citrullus Colocynthis (L.) Schrad: A Medicinal Plant, John Peter Paul J.

Ethnobotanical Leaflets

In the present study antimicrobial efficiency of Citrullus colocynthis (L.) Schrad was studied on seven bacteria Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella typhi and Staphylococcus aureus. Ethanol, chloroform and petroleum ether extracts were used for antibacterial assay. Growth inhibitions were evaluated by the disc diffusion method. Ethanol extract is more active against Escherichia coli, Proteus vulgaris and Staphylococcus aureus. Pseudomonas aeruginosa and Bacillus subtilis show moderate activity in chloroform extract. Petroleum ether extract is more active against Escherichia coli. All extracts exhibited considerably less activity against Klebsiella pneumoniae and Salmonella typhi.

In Vitro Evaluation Of Antibacterial Activity Using Crude Extracts Of Catharanthus Roseus L. (G.) Don., S. Ramya Et Al.

Ethnobotanical Leaflets

Catharanthus roseus popularly known as Madagascar periwinkle is a potential source for anti-leukemic alkaloids. The present study aims to evaluate the possibility for the presence of novel bio-active compounds against pathogenic bacteria, as most of the pathogens develop drug resistance against commonly used antibiotics. To determine antibacterial activity, crude extracts from different parts of C. roseus were tested against bacterial strains of clinical significance. Extraction of bio-active principles in appropriate solvent was followed by evaluation of antibacterial activity by disc diffusion assay against selected bacterial stains. Data depicts that the pattern of inhibition largely depends upon extraction procedure, plant part, …

Floristic And Medicinal Uses Of Some Plants Of Chandaka Denudated Forest Patches Of Bhubaneswar, Orissa, India, Kambaska Kumar Behera, Santilata Sahoo, Sanjukta Patra

Ethnobotanical Leaflets

The present paper reports 17 plant species belonging to 13 families, mostly used for various disease and disorders by the local people and herbal healer of Chandaka areas of Bhubaneswar. The local population of the region primarily depends upon these plants for curing of various aliments and their disorders. They are enumerated with binomial, family, vernacular name and floristic study and medicinal uses of the plants and their parts . Further studies on chemical and pharmacological actions are suggested to validate the claims.

Genetic Variability Of The European Corn Borer, Ostrinia Nubilalis, Suggests Gene Flow Between Populations In The Midwestern United States, Jeffrey T. Krumm, Thomas E. Hunt, Steven R. Skoda, Gary L. Hein, Donald J. Lee, Pete L. Clark, John E. Foster

Department of Entomology: Faculty Publications

The European corn borer, Ostrinia nubilalis (Hübner) (Lepidoptera: Crambidae), is a widely distributed and serious economic pest to corn production in the U.S. Genetic variability of O. nubilalis was studied in 18 sub-populations in the upper Midwestern United States using amplified fragment length polymorphism. The relatively low GST values indicate that more variation exists within populations than between populations. High gene flow (Nm) values were indicated across the entire O. nubilalis population; the lowest degree of gene flow was in the northern samples (Nm = 1.96) and the highest degree of gene flow was in the southern samples (Nm = …

Nebline, Nov./Dec. 2008

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Make a Difference: Reduce, Reuse, Recycle
2008 Crop Year in Review
Preserve Grain Quality with Aeration Management
Estimating Corn Drydown Time
It’s Not Easy Being Green During Nebraska’s Winters
Put on an Extra Blanket (Winter Mulching)
Avoid Stacking Firewood Next to House
The Keys to a Perfect Thanksgiving Meal
$tretch Your Food Dollar During the Holiday Season
Healthy Eating: Easy as Pie—Turkey Pot Pie
Family & Community Education (FCE) Clubs: Presidents’ Notes—Bonnie’s Bits
FCE News & Events
Household Hints: Keep Kitchen and Bathroom Clean During Guest Season
Skip the “Humbug!” 10 Tips to Relieve Holiday Stress
Housecleaning Ergonomics: How to …

Dietary Resistant Starch Upregulates Total Glp-1 And Pyy In A Sustained Day-Long Manner Through Fermentation In Rodents, June Zhou, Roy J. Martin, Richard T. Tulley, Anne M. Raggio, Kathleen L. Mccutcheon, Li Shen, Samuel Colby Danna, Sasmita Tripathy, Maren Hegsted, Michael J. Keenan

Faculty Publications

Glucagon-like peptide-1 (GLP-1) and peptide YY (PYY) are anti-diabetes/obesity hormones ecreted from the gut after meal ingestion. We have shown that dietary-resistant starch (RS) increased GLP-1 and PYY secretion, but he mechanism remains unknown. RS is a fermentable fiber that lowers the glycemic index of the diet and liberates short-chain fatty acids (SCFAs) through fermentation in the gut. This study investigates the two possible mechanisms by which RS stimulates GLP-1 and PYY secretion: the effect of a meal or glycemic index, and the effect of fermentation. Because GLP-1 and PYY secretions are stimulated by nutrient availability in the gut, the …

Perceptions Of School Superintendents And A Community Task Force Regarding The Health, Pedagogical, Social, And Economic Policy And Planning Decisions For Longterm School Closure Due To Pandemic Influenza-A Multi-Method Approach, David G. Duford

Education Doctoral

The research focused on the impact pandemic influenza could have on K-12 school systems. The research looked at responses of school leaders regarding the pedagogical, community health, social, and economic impacts of a pandemic. During an influenza pandemic school districts may be asked to close for as long as 12 weeks to reduce contact among children and stop the spread of disease. School superintendents' perceptions and attitudes were sought regarding school policy implications associated with long-term closure of K-12 school districts. The research methods used for this study included: (a) electronic survey, (b) face-to-face interviews, and (c) focus group. Information …

Elaborating The Ideological And Structural Content Of Women’S Work In A Globalized Economy, Jinky Leilanie Lu

Journal of International Women's Studies

Women’s employment in the global market now has been increasing as a result of globalization and internationalization of labour. This study has two main objectives in its attempt to analyze the relations of gender, the global market, and health in the workplace. First, the article tries to elucidate the structural content of work as seen in the work organization; and second, to elaborate the ideological content of work as defined by ideologies of the organization, of the state and of the market production system. The study used quantitative methodology through surveys of 630 women workers in 23 establishments, as well …

Female Adolescents’ Attitude Towards Sexually Risky Behaviors, Janet F. Wang, Patricia S. Simoni, Ying Wu, Christine Banvard

Journal of International Women's Studies

This cross-sectional survey examines the association between attitudes and female adolescents’ sexually risky behaviors. A sample of 159 subjects, aged 14 – 20 from three high schools in West Virginia participated in the study. The results indicate that 60.3% (n = 96) reported having had sexual intercourse and, of this sexually active group, 60.4% (n = 58) stated that they had sex the first time at age 15 or younger. Fifty-five percent (n = 88) reported at least one of the following behaviors: (1) sexual initiation at age 15 or younger, (2) frequent sexual intercourse last month, (3) multiple sex …

The Role Of Gender, Self-Efficacy, Age And Extroversion On Smoking Behaviour Among Ambrose Alli University Students, Ekpoma, Nigeria, S. O. Momoh, H. O. Imhonde, E. Omagbe

Journal of International Women's Studies

This study examined the role of gender, self-efficacy, age and extroversion on smoking behavior among Ambrose Alli University Students. Two hundred and fifty (250) undergraduates participated in the study (males 160 and females 90). Questionnaire was used in collecting data that were analyzed in this study. The questionnaire consisted of 4 sections: the demographic variables, extroversion scale, self-efficacy scale and attitude toward smoking scale. Two out of the four hypotheses tested were supported. Results of the independent t-test indicated that there was a significant difference in the onset of smoking between females and males (t = 2.354, df = 248, …

William Faulkner And The Mithraic Midwife, Owen Elmore

Journal of International Women's Studies

The two parts of William Faulkner’s If I Forget Thee, Jerusalem—“The Wild Palms” and “Old Man”—mirror one another; the second story reflects a more familiar way of illustrating the same theme as the first. Each story emphasizes the other, building the shared theme subversive of traditional gender roles. It is also my feeling that Faulkner inverts Arthurian motifs to tell both stories (especially “The Wild Palms,” though most assume “Old Man” to be the more romantically accessible tale) exactly as Nathaniel Hawthorne did to tell The Scarlet Letter. Hawthorne’s bifurcating of the Grail legend in order to reclaim …