Digital Commons Network^™

Scalable And Accurate Computational Frameworks For Complex Genetic Variants: From Repeat Expansion Discovery To Somatic Mosaicism Benchmarking, Zitian Tang

Arts & Sciences Graduate Student Theses and Dissertations

Whole-genome sequencing (WGS) offers a comprehensive view of genetic variation in human disease, but translating sequencing data into reliable biological and clinical insight depends on both the deployment of appropriate computational tools and the availability of rigorous benchmarking resources to evaluate them. This dissertation addresses both requirements through two complementary lines of work. In a cohort of 788 patients with idiopathic peripheral neuropathy (IPN) from the Peripheral Neuropathy Research Registry, a WGS-based pipeline integrating ExpansionHunter Denovo and ExpansionHunter with unsupervised genotype clustering identified biallelic RFC1 AAGGG repeat expansions in 2.3% of all IPN cases and 6.9% of patients with pure …

Modifying Lta Zeolites For Reversible Hydrogen Sulfide Capture And Elucidating The Structure Of The Incorporated Metal And Sulfur Ions, Adeyemi D. Ojaide

Theses and Dissertations

Hydrogen sulfide (H2S) is a highly toxic and corrosive gas that poses significant environmental and industrial challenges, particularly within the wastewater treatment and oil and gas sectors. H2S leakage is among the deadliest industrial hazards, highlighting the urgent need for the development of highly efficient, non-corrosive adsorbents. This dissertation provides an in-depth examination of the use of metal-zeolites for H2S capture. We carefully investigate the impact of multiple divalent and one monovalent transition metals on LTA zeolites to optimize H2S removal. While Linde Type A (LTA) zeolites offer a robust structural framework for gas separation, a comprehensive understanding of how …

Full Issue

The Thetean: A Student Journal for Scholarly Historical Writing

No abstract provided.

Co-Opting Modernity: The Semantic Retooling Of Faith At The 1893 World’S Parliament Of Religions, Erik P. Palmer

The Thetean: A Student Journal for Scholarly Historical Writing

The 1893 World’s Parliament of Religions in Chicago served as a high-stakes arena where the boundaries of religious legitimacy were redrawn through the intentional adoption of social science vocabulary. Staged within the Neo-Classical architecture of the White City of the World’s Columbian Exposition, the event was more than a civil gathering; it functioned as a “Museum of Faiths” where religious traditions were curated, categorized, and evaluated against the perceived benchmarks of Western civilization. The physical environment of the Fair, a monument to industrial triumph, was built to display things that worked. The organizers demanded that religions demonstrate they were useful …

Reshaping Home: Chinese Immigrant Women’S Adaptation And Family Transformation Under Exclusion, Chloe Nicole 零星 Isom

The Thetean: A Student Journal for Scholarly Historical Writing

In 1941, Jew Law Ying arrived at the port of San Francisco with her infant daughter, Bak Heong, seeking admission to join her merchant husband, Yung Hin Sen, in the United States. Upon arrival, immigration officials detained Jew Law Ying and her daughter and required both her and her husband to answer nearly two hundred detailed questions about themselves to prove their identities and relationship. Officials designed these questions to uncover inconsistencies and expose fraudulent relationships, and they were so intricate that, despite their legal and legitimate marriage, Jew Law Ying and Yung Hin Sen had prepared extensively, memorizing answers …

Assessing Non-Thermal Plasma In Cutaneous Regeneration, Arden Rodriguez, Meliah Martin, Graeme Grainger, Arineh Shahbazi

Discovery Day - Daytona Beach

Chronic and acute wounds remain a significant clinical challenge, particularly in austere and resource-limited environments where access to advanced care is restricted. Non-Thermal Plasma (NTP) has emerged as a promising, non-invasive modality with reported antimicrobial and regenerative properties; however, its mechanisms of action and effects on human skin tissue require further characterization. The scope of this project is to engineer and utilize a 3D human skin epithelial model to evaluate the effects of NTP on wound healing. We will first build a 3D epithelial tissue model that will simulate human skin, followed by modeling and evaluation of wound pathology and …

Advanced Manufacturing And Materials Of A Bio-Mimetic Hoof For Space Robotics Applications, Benjamin Heckel

Discovery Day - Daytona Beach

Planetary surfaces often feature steep, uneven, and unpredictable terrain conditions that resemble some of the most challenging natural environments on Earth. In these terrestrial settings, certain animals have evolved to navigate complex landscapes with remarkable agility. One example is the mountain goat, an animal whose hooves have evolved for stable and precise movement across rocky, inclined surfaces. This biological adaptation provides a useful model for improving robotic mobility in planetary exploration. This study draws inspiration from that natural adaptation to develop a bio-mimetic robotic hoof designed to enhance traction, durability, and adaptability for extraterrestrial exploration. By replicating the mechanical properties …

Steam Distillation Star Anise, Makayla Maso, Kiara Maso

Discovery Day - Daytona Beach

Star anise (Illicium verum) contains several volatile organic compounds responsible for its characteristic aroma, including trans-anethole, estragole, limonene, and linalool. These compounds differ in structure and chemical properties, which influence their behavior during isolation and analysis. Trans-anethole and estragole are classified as aromatic phenylpropene derivatives that contain benzene rings and ether functionalities. In contrast, limonene and linalool are non-aromatic terpenes, with linalool additionally possessing an alcohol functional group. These compounds are widely utilized as flavoring agents and fragrance components, while terpenes such as limonene and linalool are also commonly employed as solvents and in formulation chemistry due to their volatility …

The Osteocyte-Osteoblast Network Requires Dynamic Mechanical Loading For Key Functions, Kevin Kowalski

Discovery Day - Daytona Beach

Spaceflight investigations have demonstrated that reduction in gravity-induced loading regimes is detrimental to maintenance Earth-normal bone and marrow. Mechanistically, however, there are still gaps in knowledge about how the reduction in gravity influences the different niches within the tissue. For example, cortical bone is a stable semi-solid structure that allows for only minor deformations before failure, while marrow is a viscous hydrogel-like material that can resist significant fluidic and compressive forces. We hypothesize that maintenance of different niches within the bone microenvironment are dependent on different loading regimes, and that the collective impact if gravity-induced loading needs to be considered …

Modeling Population Growth With Logistic And Modified Logistic Equations, Mihil Dimpal Patel

Discovery Day - Daytona Beach

Population growth models are essential tools for understanding how biological populations change over time under environmental constraints. This study examines population dynamics by comparing the classical exponential growth model with the logistic growth model. While exponential growth assumes unlimited resources and results in unbounded population increase, the logistic model incorporates a carrying capacity that limits growth as resources become scarce. To better represent real-world conditions, the logistic model is extended by introducing modifications such as harvesting terms and time-varying carrying capacities, which account for external removal of individuals and changing environmental limits. The equilibria of these models are determined, and …

Polymer Degradation And Their Environmental Impact, Addison Orr, Vanessa Gilbert

Discovery Day - Daytona Beach

Synthetic polymer material usage has increased significantly within the last several decades due to their durability, resistance to mechanical wear, low density, and inexpensiveness. Despite these advantages, most synthetic polymers are not biodegradable and instead undergo slow degradation processes. The processes are multifaceted, involving physical, chemical, and biological mechanisms influenced by material properties and environmental conditions, such as soil, light, temperature, and moisture levels. As a result, the polymers break into microplastics and contaminate air, water, and soil, posing risks to human health and surrounding ecosystems. Additionally, polymer degradation can release harmful substances that disrupt food chains and can lead …

Production And Analysis Of Lavender Essential Oil: Extraction, Composition, And Applications, Mary Zoeller

Discovery Day - Daytona Beach

Steam distillation was performed to extract essential oil from lavender and analyze its chemical composition. Lavender is known to contain several compounds such as linalool, terpinen-4-ol, and linalyl acetate, which contribute to its characteristic aroma and biological activity. The purpose of this experiment was to isolate these compounds without decomposition and identify them using gas chromatography–mass spectrometry (GC-MS). Approximately 14.851 g of lavender was heated with water to generate steam, which carried the volatile compounds into a condenser where the vapor was cooled and collected. The distillate was transferred to a separatory funnel to isolate the organic layer from the …

Bridging Discrete And Continuous Systems: Fibonacci Sequences And Exponential Growth From Odes, Martyna Wojcik

Discovery Day - Daytona Beach

Bridging Discrete and Continuous Systems: Fibonacci Sequences and Exponential Growth from Ordinary Differential Equations       It has been observed that nature often exhibits specific patterns of growth and structure in biological systems and spiral formations. The Fibonacci sequence, defined as a discrete recursive sequence where each term is generated as the sum of the two preceding terms, “has been applied extensively to understand some natural phenomena” (Pakdemirli, 2023). In contrast, exponential growth describes a continuous process in which the rate of change of a quantity is proportional to its current value. Such behavior is modeled using differential equations that “produce solutions …

Using H2ax As A Biological Indicator To Assess Radiation-Induced Bone Loss In Microgravity Environments, Angeline Nichols, Enoch Tonkin, Ana Lobaton, Jahzara Hope

Discovery Day - Daytona Beach

Long-duration space missions expose astronauts to microgravity and chronic low-dose radiation, both greatly impacting skeletal health. While mechanical unloading in microgravity is a well-known driver of bone loss, recent evidence indicates that radiation independently contributes to bone deterioration by increasing osteoclast activity, which remodel bone faster, and suppress osteoblast differentiation. H2AX, a histone protein that rapidly phosphorylates into y-H2AX in response to the double strands of DNA breaking, has emerged as a sensitive biological marker for radiation exposure. This project aims to determine the efficacy of H2AX in the presence of radiation-induced DNA damage in bone marrow, compared to microgravity …

Analysis Of Non-Thermal Plasma’S Effects On Melanoma: A Biological Examination, Violet Ochrietor, Mia Nilsen, Arden Rodriguez, Ally Schwennesen

Discovery Day - Daytona Beach

Melanoma is a serious form of skin cancer that may present as a real and increased risk during long-term spaceflight due to radiation exposure and limited treatment options. Non-Thermal Plasma (NTP) has emerged as a promising therapeutic tool due to its ability to generate reactive oxygen and nitrogen species (ROS and RNS) that affect cellular functioning without causing temperature related damage to surrounding tissues. This project investigates the effects of NTP on melanoma cells through a biological examination of exposure time, frequency of plasma, and cellular growth conditions. Human melanoma cells and keratinocytes will be cultured and exposed to the …

Exercise Intensity And The Effects On Mood And Wellbeing, Emily Amortegui, Ashley Albert, Riley Ensor, Keyanna Keyanna Jackson

Discovery Day - Daytona Beach

With many students dealing with anxiety, sadness, and high levels of stress, mental health issues among college students have grown in intensity. A lot of research has been done on the possible benefits of physical activity for mental health, but most of it focuses on correlations rather than the biological mechanisms. The purpose of this study is to examine the effects of varying exercise intensities on physiological stress markers and mood in college students ages 18 to 25. To assure precise intensity levels, participants from Embry-Riddle Aeronautical University will work out at low, moderate, and high intensities while wearing heart …

Steam Distillation Of Jasmine, Mikayla Hulvey, Meliah Martin

Discovery Day - Daytona Beach

This experiment aimed to isolate various components, including benzyl acetate, linalool, indole, and farnesene, from jasmine buds. The functional groups include an ester in benzyl acetate, an alcohol in linalool, an amine in indole, and alkenes in farnesene. Jasmine buds were combined with distilled water and steam distilled using a standard distillation apparatus. The distillate, containing hydrosol and trace amounts of essential oil, was then collected for extraction using methyl tert-butyl (MTBE) for a liquid-liquid extraction. The organic layer was then separated, washed using brine, dried over anhydrous sodium sulfate, and then transferred for later analysis. The essential oil obtained …

The Algorithm Of Difference: Gender, Communication, And Perception Online, Samantha Garcia

Discovery Day - Daytona Beach

This in-progress independent study thesis examines how exposure to gender-targeted content on social media platforms shapes college-aged users’ perceptions of communication differences between men and women. As platforms such as TikTok and Instagram increasingly rely on algorithmic curation, users are exposed to repetitive and often polarized narratives about gender roles, dating expectations, and interpersonal communication. This study builds on genderlect theory, as introduced by Deborah Tannen, while also incorporating more contemporary perspectives that frame gender as socially constructed and culturally performed rather than biologically determined. The research addresses the question of whether algorithm-driven content reinforces, reshapes, or distorts perceived communication …

In Memoriam: Claudette Colvin, Luke Hunter

History in the Making

No abstract provided.

The Impact Of Technology On The Chinese Queer Community, Samantha Collasco

History in the Making

This project’s aim is to analyze the various ways in which the internet has influenced the Chinese LGBTQ+ community. The Chinese government has created an immense censorship over the community and any accompanying terms, therefore leading to their discrimination and marginalization within Chinese society. How has queer representation changed in China since the decriminalization of homosexuality? How has the internet affected the public perception of the LGBTQ+ community? What role does censorship play in the shaping of public perception of queer identities? How has westernization and globalization affected the attitudes towards queer identities? This work engages with the existing scholarship …

The Connection: American Influence On Nazi Racial Hygiene, Dylan O’Connor, Elizabeth Vazquez

History in the Making

This paper analyzes the American eugenics movement’s significant role in shaping Nazi policies, by providing institutional models, ideological inspiration, scientific justification, and legal frameworks that the Third Reich expanded on to pursue its racial purification goals. First, this article traces how the eugenic movement developed from Francis Galtons’s theories to fully established organizations and independent groups such as the Eugenics Record Office and the American Eugenics Society. The paper further examines figures like Charles B. Davenport and Harry Laughlin, who influenced American sterilization laws and immigration restrictions, as well as Ernst Rubin, Eugen Fischer, and Fitz Lenz German, racial hygienists …

Exploring The Motivations Of Undergraduate Sport Management Students, Kyle J. Brannigan, Tyler J. Hajek

The COSMA Journal

This study explored the motivations of undergraduate sport management in four university programs. Convergent mixed-methods research through the theoretical framework of Self-Determination Theory was incorporated with an adapted questionnaire of the Academic Motivation Scale and semi-structured interviews through Thematic Analysis. Ninety-six surveys with nine interviews were deemed usable across four regionally focused, vocationally minded, and accessible universities. Overall, students reported higher levels of extrinsic motivation than intrinsic motivation. The findings suggested intrinsic motivation to know and extrinsic motivation through regulation for identification scored highest with the participants. No significant differences were found between classes and ethnicities. However, male students experienced …

Caldwell Clean‑Water Engine (Ccwe‑1.0) Unified Cavitation‑Plasma, Microbubble, Atmospheric, And Polishing Architecture For Pfas, Microplastic, Pathogen, And Brine‑Grade Contaminant Reduction, Michael Victor Caldwell Mr.

Defensive Publications Series

CCWE‑1.0 is a modular, container‑scale clean‑water engine that accepts:

• Floodwater

• Sewage‑contaminated surface water

• Industrial brines

• Saline wells

• Atmospheric humidity

and outputs:

• Potable water (where validated)

• Decontaminated non‑potable water (for industrial/ag use)

• Mapped waste streams (sludge, spent media, brine)

It integrates:

• Hydrodynamic cavitation (CPR‑1.0)

• Plasma‑assisted destruction (CPR‑1.0)

• Microbubble oxidation (MBR‑1.0)

• Conventional filtration and adsorption (POL‑1.0)

• Atmospheric water harvesting (AWH‑1.0)

• Telemetry and safety control (CTL‑1.0)

• Waste stream handling (WST‑1.0)

• Power and structural backbone (PWR‑1.0)

All subsystems use standard industrial components and conservative operating envelopes so labs, utilities, and humanitarian teams can build, test, and iterate without proprietary parts or hidden parameters.

Mainehealth Investigator Vignettes - August 2026

Journal of Maine Medical Center

No abstract provided.

Behavioral Effects Of Novel Treatments For Pain Management Using Combinatorial Methods, Rohit Rajeev Nambiar

Theses and Dissertations

Pain, despite being an uncomfortable feeling to experience, is a necessary protective mechanism for the body1. Understanding that pain results from several different mechanisms within the body is important to discovering the optimal way to treat and manage a person’s pain. Modern medicine has seen numerous advancements in treating or managing acute and chronic pain, particularly with opioid drugs. However, these treatments come with potential side effects, including opioid use disorder (OUD) and fatal overdose. Finding new treatment methods, or improving existing methods, is crucial for patients with pain. This thesis explores two projects looking to develop safer analgesia using …

Acute Effect Of Honey-Sweetened Green Tea On Blood Glucose, Blood Pressure And Heart Rate In Healthy Female Subjects: A Preliminary Randomized Controlled Study, Esther Oluwasola Aluko, Patience John Okon, Mary Emmanuel Etim, Ernest Etim Akpan

Asian Medical Journal and Alternative Medicine

Introduction: Green tea contains bioactive catechins associated with antioxidant and cardiometabolic benefits, while honey is a natural sweetener rich in phenolic compounds with potential health-promoting properties. However, limited data exists on the combined physiological effects of green tea sweetened with honey.

Objectives: This study assessed the acute effects of honey-sweetened green tea on blood glucose, blood pressure, and heart rate in healthy young adult females.

Methods: Thirty healthy females aged 18–25 years participated in this randomized controlled study conducted over three consecutive days. Participants were randomly assigned into three groups: control group (250 mL warm water), green tea group (250 …

Melatonin In Pediatric Critical Care: From Physiology To Bedside Roles, Chanapai Chaiyakulsil

Asian Medical Journal and Alternative Medicine

Melatonin is an endogenous indoleamine synthesized by the pineal gland. Though melatonin is best known for its chronobiologic effects, regulating the circadian rhythm and sleep, it might play a wider role in critical illness. It has been proposed for use as an analgesic and sedative, an antihypertensive, an anti-inflammatory, and for the prevention of delirium. Melatonin use in children has increased dramatically over the years, but the evidence on its use in critically ill children remains limited. This review article aimed to describe the basic physiology and biology of melatonin, including its production and functions, dosing, and side effects, …

Translational Reading Frame Predicts The Pathogenicity Of C-Terminal Frameshift Deletions In Mecp2, Jacky Guy, Elena Hein, Beatrice Alexander-Howden, Timur Von Bock Und Polach, Tricia Mathieson, Benjamin P Kleinstiver, Huda Y Zoghbi, Adrian Bird

Duncan NRI Faculty and Staff Publications

Mutations in the MECP2 gene cause the severe neurological disorder Rett syndrome. A cluster of frameshift-causing C-terminal deletions (CTDs) removes ~100 amino acids and accounts for approximately 10% of RTT-causing mutations. Their pathogenicity is unexpected because this C-terminal domain is dispensable in mice. Analysis of pathogenic and benign human MECP2 variants reveals that some individuals with apparently typical CTDs do not develop Rett syndrome, confirming that C-terminal truncations are not intrinsically pathogenic. Using human sequence data and mouse models we show that pathogenicity results from a marked reduction in MeCP2 levels and depends on the presence of a proline proline …

Vitamin D As A Candidate Host Susceptibility Factor In Sugar-Driven Oral Dysbiosis, Moldir Turaliyeva, Nuraly S. Akimbekov, Ilya Digel, Adil Y. Yerezhepov, Mohammed Razzaque

School of Medicine Publications

Introduction: Sugar-sweetened beverages (SSBs) represent a modifiable dietary factor that promotes oral dysbiosis by supplying fermentable sugars and generating low-pH conditions that favor acidogenic and acid-tolerant biofilms. Vitamin D plays a biologically significant role in oral host defense through regulation of the epithelial barrier, induction of antimicrobial peptides, immune modulation, inflammatory control, and mineral metabolism. However, the extent to which vitamin D modifies the SSB -oral microbiome -disease pathway in humans remains unclear.

Methods: This narrative-conceptual review employed structured evidence mapping. Relevant literature was identified through PubMed/MEDLINE, Scopus, and Web of Science Core Collection, with additional sources obtained via citation …

E3 Ligase Rnf145 Regulates Airway Smooth Muscle Cell Proliferation Via The Pp2a-P70s6k Signaling Axis, Vijaya Kumar Gangipangi, Erin O. Curley, Deepak A. Deshpande, Pawan Sharma

Center for Translational Medicine Faculty Papers

Airway smooth muscle (ASM) is a key determinant of airway caliber and a major contributor to structural remodeling in obstructive lung diseases, including asthma and COPD. ASM proliferation is regulated by transcriptional and post-transcriptional mechanisms, including ubiquitin-dependent protein turnover mediated by E3 ubiquitin ligases. Here, we investigated the role of the RING-type E3 ligase RNF145 in mitogenic signaling and ASM cell proliferation. Human ASM cells were transfected with RNF145 shRNA or treated with the E3 ligase inhibitor SMER3, followed by stimulation with fetal bovine serum (FBS) or platelet-derived growth factor (PDGF). RNF145 knockdown or SMER3 treatment dose-dependently inhibited mitogen-induced ASM …