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Articles 11431 - 11460 of 14126
Full-Text Articles in Entire DC Network
Heteropolymer Translocation Through Nanopores, Kaifu Luo, Tapio Ala-Nissila, See-Chen Ying, Aniket Bhattacharya
Heteropolymer Translocation Through Nanopores, Kaifu Luo, Tapio Ala-Nissila, See-Chen Ying, Aniket Bhattacharya
Faculty Bibliography 2000s
The authors investigate the translocation dynamics of heteropolymers driven through a nanopore using a constant temperature Langevin thermostat. Specifically, they consider heteropolymers consisting of two types of monomers labeled A and B, which are distinguished by the magnitude of the driving force that they experience inside the pore. From a series of studies on polymers with sequences A(m)B(n) the authors identify both universal as well as specific sequence properties of the translocating chains. They find that the scaling of the average translocation time as a function of the chain length N remains unaffected by the heterogeneity, while the residence time …
Inhibition Of Hydrogen Uptake In Escherichia Coli By Expressing The Hydrogenase From The Cyanobacterium Synechocystis Sp Pcc 6803, Toshinari Maeda, Gönül Vardar, William T. Self, Thomas K. Wood
Inhibition Of Hydrogen Uptake In Escherichia Coli By Expressing The Hydrogenase From The Cyanobacterium Synechocystis Sp Pcc 6803, Toshinari Maeda, Gönül Vardar, William T. Self, Thomas K. Wood
Faculty Bibliography 2000s
Background: Molecular hydrogen is an environmentally- clean fuel and the reversible ( bidirectional) hydrogenase of the cyanobacterium Synechocystis sp. PCC 6803 as well as the native Escherichia coli hydrogenase 3 hold great promise for hydrogen generation. These enzymes perform the simple reaction 2H(+) + 2e-. H-2 ( g). Results: Hydrogen yields were enhanced up to 41- fold by cloning the bidirectional hydrogenase ( encoded by hoxEFUYH) from the cyanobacterium into E. coli. Using an optimized medium, E. coli cells expressing hoxEFUYH also produced twice as much hydrogen as the well- studied Enterobacter aerogenes HU- 101, and hydrogen gas bubbles are …
Modulation Of Human Neural Stem Cell Differentiation In Alzheimer (App23) Transgenic Mice By Phenserine, Amelia Marutle, Masao Ohmitsu, Mats Nilbratt, Nigel H. Greig, Agneta Nordberg, Kiminobu Sugaya
Modulation Of Human Neural Stem Cell Differentiation In Alzheimer (App23) Transgenic Mice By Phenserine, Amelia Marutle, Masao Ohmitsu, Mats Nilbratt, Nigel H. Greig, Agneta Nordberg, Kiminobu Sugaya
Faculty Bibliography 2000s
In a previous study, we found that human neural stem cells (HNSCs) exposed to high concentrations of secreted amyloid-precursor protein (sAPP) in vitro differentiated into mainly astrocytes, suggesting that pathological alterations in APP processing during neurodegenerative conditions such as Alzheimer's disease (AD) may prevent neuronal differentiation of HNSCs. Thus, successful neuroplacement therapy for AD may require regulating APP expression to favorable levels to enhance neuronal differentiation of HNSCs. Phenserine, a recently developed cholinesterase inhibitor (ChEI), has been reported to reduce APP levels in vitro and in vivo. In this study, we found reductions of APP and glial fibrillary acidic protein …
Invariant Resolution Dynamic Focus Ocm Based On Liquid Crystal Lens, S. Murali, K. S. Lee, J. P. Rolland
Invariant Resolution Dynamic Focus Ocm Based On Liquid Crystal Lens, S. Murali, K. S. Lee, J. P. Rolland
Faculty Bibliography 2000s
A primary limitation of optical coherence microscopy is the lack of sufficient lateral resolution over a usable imaging volume for diagnostic applications, even with high-numerical aperture imaging optics. In this paper, we first motivate the benefit of refocusing at multiple depths in a highly scattering biological sample using optical coherence microscopy, which experimentally shows invariant 2.5 mu m axial and 6.5 mu m lateral resolution throughout the sample. We then present the optical system design of a hand-held probe with the advanced capability to dynamically focus with no moving parts to a depth of 2 mm in skin-equivalent tissue at …
Pet, A Non-Ab Toxin, Is Transported And Translocated Into Epithelial Cells By A Retrograde Trafficking Pathway, Fernando Navarro-García, Adrián Canizalez-Roman, Kaitlin E. Burlingame, Ken Teter, Jorge E. Vidal
Pet, A Non-Ab Toxin, Is Transported And Translocated Into Epithelial Cells By A Retrograde Trafficking Pathway, Fernando Navarro-García, Adrián Canizalez-Roman, Kaitlin E. Burlingame, Ken Teter, Jorge E. Vidal
Faculty Bibliography 2000s
The plasmid-encoded toxin (Pet) of enteroaggregative Escherichia coli is a 104-kDa autotransporter protein that exhibits proteolytic activity against the actin-binding protein alpha-fodrin. Intracellular cleavage of epithelial fodrin by Pet disrupts the actin cytoskeleton, causing both cytotoxic and enterotoxic effects. Intoxication requires the serine protease activity of Pet and toxin endocytosis from clathrin-coated pits. The additional events in the intracellular trafficking of Pet are largely uncharacterized. Here, we determined by confocal microscopy that internalized Pet is transferred from the early endosomes to the Golgi apparatus and then travels to the endoplasmic reticulum (ER). Pet associates with the Sec61p translocon before it …
Liquid-Crystal-Based Linear Polarization Rotator, Hongwen Ren, Shin-Tson Wu
Liquid-Crystal-Based Linear Polarization Rotator, Hongwen Ren, Shin-Tson Wu
Faculty Bibliography 2000s
A liquid-crystal (LC)-based polarization rotator which can rotate the polarization axis of an incident linearly polarized light from 0 degrees to 90 degrees is demonstrated. In the LC cell, the top substrate has a uniform rubbing but the bottom substrate has two orthogonal rubbings which are separated by a nonrubbing zone. Between these two rubbed strips, the LC directors twist continuously from 0 degrees to 90 degrees. As a result, the optic axis of the incident linearly polarized light can be rotated continuously depending on the beam position.
Geographical Gradients In Diet Affect Population Dynamics Of Canada Lynx, James D. Roth, John D. Marshall, Dennis L. Murray, David M. Nickerson, Todd D. Steury
Geographical Gradients In Diet Affect Population Dynamics Of Canada Lynx, James D. Roth, John D. Marshall, Dennis L. Murray, David M. Nickerson, Todd D. Steury
Faculty Bibliography 2000s
Geographical gradients in the stability of cyclic populations of herbivores and their predators may relate to the degree of specialization of predators. However, such changes are usually associated with transition from specialist to generalist predator species, rather than from geographical variation in dietary breadth of specialist predators. Canada lynx (Lynx canadensis) and snowshoe hare (Lepus americanus) populations undergo cyclic. fluctuations in northern parts of their range, but cycles are either greatly attenuated or lost altogether in the southern boreal forest where prey diversity is higher. We tested the influence of prey specialization on population cycles by measuring the stable carbon …
Expression Of Cholera Toxin B-Proinsulin Fusion Protein In Lettuce And Tobacco Chloroplasts - Oral Administration Protects Against Development Of Insulitis In Non-Obese Diabetic Mice, Tracey Ruhlman, Raheleh Ahangari, Andrew Devine, Mohtahsem Samsam, Henry Daniell
Expression Of Cholera Toxin B-Proinsulin Fusion Protein In Lettuce And Tobacco Chloroplasts - Oral Administration Protects Against Development Of Insulitis In Non-Obese Diabetic Mice, Tracey Ruhlman, Raheleh Ahangari, Andrew Devine, Mohtahsem Samsam, Henry Daniell
Faculty Bibliography 2000s
Lettuce and tobacco chloroplast transgenic lines expressing the cholera toxin B subunit-human proinsulin (CTB-Pins) fusion protein were generated. CTB-Pins accumulated up to similar to 16% of total soluble protein (TSP) in tobacco and up to similar to 2.5% of TSP in lettuce. Eight milligrams of powdered tobacco leaf material expressing CTB-Pins or, as negative controls, CTB-green fluorescent protein (CTB-GFP) or interferon-GFP (IFN-GFP), or untransformed leaf, were administered orally, each week for 7 weeks, to 5-week-old female non-obese diabetic (NOD) mice. The pancreas of CTB-Pins-treated mice showed decreased infiltration of cells characteristic of lymphocytes (insulitis); insulin-producing beta-cells in the pancreatic islets …
The Complete Nucleotide Sequence Of The Coffee (Coffea Arabica L.) Chloroplast Genome: Organization And Implications For Biotechnology And Phylogenetic Relationships Amongst Angiosperms, Nalapalli Samson, Michael G. Bausher, Seung-Bum Lee, Robert K. Jansen, Henry Daniell
The Complete Nucleotide Sequence Of The Coffee (Coffea Arabica L.) Chloroplast Genome: Organization And Implications For Biotechnology And Phylogenetic Relationships Amongst Angiosperms, Nalapalli Samson, Michael G. Bausher, Seung-Bum Lee, Robert K. Jansen, Henry Daniell
Faculty Bibliography 2000s
The chloroplast genome sequence of Coffea arabica L., the first sequenced member of the fourth largest family of angiosperms, Rubiaceae, is reported. The genome is 155 189 bp in length, including a pair of inverted repeats of 25 943 bp. Of the 130 genes present, 112 are distinct and 18 are duplicated in the inverted repeat. The coding region comprises 79 protein genes, 29 transfer RNA genes, four ribosomal RNA genes and 18 genes containing introns (three with three exons). Repeat analysis revealed five direct and three inverted repeats of 30 bp or longer with a sequence identity of 90% …
Selective Chemical Probe Inhibitor Of Stat3, Identified Through Structure-Based Virtual Screening, Induces Antitumor Activity, Khandaker Siddiquee, Shumin Zhang, Wayne C. Guida, Michelle A. Blaskovich, Benjamin Greedy, Harshani R. Lawrence, M. L. Richard Yip, Richard Jove, Mark M. Mclaughlin, Nicholas J. Lawrence, Said M. Sebti, James Turkson
Selective Chemical Probe Inhibitor Of Stat3, Identified Through Structure-Based Virtual Screening, Induces Antitumor Activity, Khandaker Siddiquee, Shumin Zhang, Wayne C. Guida, Michelle A. Blaskovich, Benjamin Greedy, Harshani R. Lawrence, M. L. Richard Yip, Richard Jove, Mark M. Mclaughlin, Nicholas J. Lawrence, Said M. Sebti, James Turkson
Faculty Bibliography 2000s
S31-201 (NSC 74859) is a chemical probe inhibitor of Stat3 activity, which was identified from the National Cancer Institute chemical libraries by using structure-based virtual screening with a computer model of the Stat3 SH2 domain bound to its Stat3 phosphotyrosine peptide derived from the x-ray crystal structure of the Stat3 beta homodimer. S31-201 inhibits Stat3-Stat3 complex formation and Stat3 DNA-binding and transcriptional activities. Furthermore, S31-201 inhibits growth and induces apoptosis preferentially in tumor cells that contain persistently activated Stat3. Constitutively climerized and active Stat3C and Stat3 SH2 domain rescue tumor cells from S31-201-induced apoptosis. Finally, S31-201 inhibits the expression of …
Calculation Of Critical Values For Somerville's Fdr Procedures, Paul N. Somerville
Calculation Of Critical Values For Somerville's Fdr Procedures, Paul N. Somerville
Faculty Bibliography 2000s
A Fortran 95 program has been written to calculate critical values for the step-up and step-down FDR procedures developed by Somerville (2004). The program allows for arbitrary selection of number of hypotheses, FDR rate, one- or two-sided hypotheses, common correlation coefficient of the test statistics and degrees of freedom. An MCV (minimum critical value) may be specified, or the program will calculate a specified number of critical values or steps in an FDR procedure. The program can also be used to efficiently ascertain an upper bound to the number of hypotheses which the procedure will reject, given either the values …
Liquid Mixing Driven Motions Of Floating Macroscopic Objects, Ming Su
Liquid Mixing Driven Motions Of Floating Macroscopic Objects, Ming Su
Faculty Bibliography 2000s
Dropping miscible and low-density organic solvents into water generates translational and rotational motions of floating objects including oil droplets, polymer half spheres, and model boats. The moving speed of the boat at different loads and the force produced by solvent drops are measured. In contrast to motions driven by surface tension of monolayer, the liquid mixing driven motion can be dynamically steered without restriction and continued provided the supply is maintained and the amount of water is large enough. Such motions are the result of Marangoni instability in binary liquid-liquid systems with intentionally produced concentration gradients behind the floating objects.
Efficacy, Pharmacokinetics, And Metabolism Of Tetrahydroquinoline Inhibitors Of Plasmodium Falciparum Protein Farnesyltransferase, Wesley C. Voorhis, Kasey L. Rivas, Pravin Bendale, Laxman Nallan, Carolyn Hornéy, Lynn K. Barrett, Kevin D. Bauer, Brian P. Smart, Sudha Ankala, Oliver Hucke, Christophe L. M. J. Verlinde, Debopam Chakrabarti, Corey Strickland, Kohei Yokoyama, Frederick S. Buckner, Andrew D. Hamilton, David K. Williams, Louis J. Lombardo, David Floyd, Michael H. Gelb
Efficacy, Pharmacokinetics, And Metabolism Of Tetrahydroquinoline Inhibitors Of Plasmodium Falciparum Protein Farnesyltransferase, Wesley C. Voorhis, Kasey L. Rivas, Pravin Bendale, Laxman Nallan, Carolyn Hornéy, Lynn K. Barrett, Kevin D. Bauer, Brian P. Smart, Sudha Ankala, Oliver Hucke, Christophe L. M. J. Verlinde, Debopam Chakrabarti, Corey Strickland, Kohei Yokoyama, Frederick S. Buckner, Andrew D. Hamilton, David K. Williams, Louis J. Lombardo, David Floyd, Michael H. Gelb
Faculty Bibliography 2000s
New antimalarials are urgently needed. We have shown that tetrahydroquinoline (THQ) protein farnesyltransferase (PFr) inhibitors (PFTIs) are effective against the Plasmodium falciparum PFr and are effective at killing P. falciparum in vitro. Previously described THQ PFTIs had limitations of poor oral bioavailability and rapid clearance from the circulation of rodents. In this paper, we validate both the Caco-2 cell permeability model for predicting THQ intestinal absorption and the in vitro liver microsome model for predicting THQ clearance in vivo. Incremental improvements in efficacy, oral absorption, and clearance rate were monitored by in vitro tests; and these tests were followed up …
Free Energy Of Dna Duplex Formation On Short Oligonucleotide Microarrays, Li Zhang, Chunlei Wu, Roberto Carta, Haitao Zhao
Free Energy Of Dna Duplex Formation On Short Oligonucleotide Microarrays, Li Zhang, Chunlei Wu, Roberto Carta, Haitao Zhao
Faculty Bibliography 2000s
DNA/DNA duplex formation is the basic mechanism that is used in genome tiling arrays and SNP arrays manufactured by Affymetrix. However, detailed knowledge of the physical process is still lacking. In this study, we show a free energy analysis of DNA/DNA duplex formation these arrays based on the positional-dependent nearest-neighbor (PDNN) model, which was developed previously for describing DNA/RNA duplex formation on expression microarrays. Our results showed that the two ends of a probe contribute less to the stability of the duplexes and that there is a microarray surface effect on binding affinities. We also showed that free energy cost …
Characteristics Of Academic Writing In Education, Andrew Kemp
Characteristics Of Academic Writing In Education, Andrew Kemp
Electronic Theses and Dissertations
According to Stangl (1994), Jalongo (2002), Richards and Miller (2005) and a host of other authors regarding publishing in educational journals, understanding the audience for an article is of utmost importance. Huff (1999) notes that an author must understand the audience for whom s/he writes. While much of this understanding of audience comes down to suitable topics (Silverman, 1982), articles must also fit the style of the journal to which it is being presented (Olsen, 1997). With this in mind, the purpose of this study is to characterize the writing style of academic writing in education. This research will involve …
Recognizing Teamwork Activity In Observations Of Embodied Agents, Linus Jan Luotsinen
Recognizing Teamwork Activity In Observations Of Embodied Agents, Linus Jan Luotsinen
Electronic Theses and Dissertations
This thesis presents contributions to the theory and practice of team activity recognition. A particular focus of our work was to improve our ability to collect and label representative samples, thus making the team activity recognition more efficient. A second focus of our work is improving the robustness of the recognition process in the presence of noisy and distorted data. The main contributions of this thesis are as follows: We developed a software tool, the Teamwork Scenario Editor (TSE), for the acquisition, segmentation and labeling of teamwork data. Using the TSE we acquired a corpus of labeled team actions both …
Human Sexuality Education In The Middle Grades Classroom: A Review Of Curricula In A Sample Of Florida School Districts, Melinda Myrick
Human Sexuality Education In The Middle Grades Classroom: A Review Of Curricula In A Sample Of Florida School Districts, Melinda Myrick
Electronic Theses and Dissertations
This study examined the extent to which human sexuality topics are covered in Florida middle school science classrooms and the process by which curricular decisions are made regarding human sexuality education on a county-wide basis. Primary data included interviews with county-level administrators who oversee curricular decisions related to the middle-grades science curriculum or health curriculum in twelve school districts within the state. These districts represented four geographic locations and districts of various sizes. Administrators from four of the twelve studies in the sample chose to provide information regarding their human sexuality education curriculum. In two cases, teacher leads were identified …
Examining Sociomathematical Norms Within The Context Of Decimals And Fractions In A Sixth Grade Classroom, Marino Nardelli
Examining Sociomathematical Norms Within The Context Of Decimals And Fractions In A Sixth Grade Classroom, Marino Nardelli
Electronic Theses and Dissertations
Social norms are patterns of behavior expected within a particular society in a given situation. Social norms can be shared belief of what is normal and acceptable shapes and enforces the actions of people in a society. In the educational classroom, they are characteristics that constitute the classroom participation structure. Sociomathematical norms are fine-grained aspects of general social norms specifically related to mathematical practices. These can include, but are not limited to, a student-centered classroom that includes the expectation that the students should present their solution methods by describing actions on mathematical objects rather than simply accounting for calculational manipulations. …
A Novel Setup For High-Pressure Raman Spectroscopy Under A Microscope, Thomas Andrew Oakeson
A Novel Setup For High-Pressure Raman Spectroscopy Under A Microscope, Thomas Andrew Oakeson
Electronic Theses and Dissertations
Functional properties of biological molecules and cells are affected by environmental parameters such as temperature and pressure. While Raman spectroscopy provides an intrinsic probe of molecular structural changes, the incorporation of a microscope enables studies of minuscule amounts of biological compounds with spatial resolution on a micron scale. We have developed a novel setup which combines a Raman microscope and a high pressure cell. A micro-capillary made out of fused silica simultaneously serves as the supporting body and the optical window of the pressure cell. The cell has been tested over the pressure range from 0.1 to 4 kbar. Raman …
Analysis Of Mitochondrial Dna Coding Region Snps By Pyrosequencing, Kyle Robert Carl Parker
Analysis Of Mitochondrial Dna Coding Region Snps By Pyrosequencing, Kyle Robert Carl Parker
Electronic Theses and Dissertations
To date, the use of mitochondrial DNA in forensic analysis has relied on the presence of variations in the control region to differentiate between samples. One problem that this analysis has shown is the occurrence of common Haplogroup H haplotypes or identical sequences. Thus, there is a need to enhance the distinguishing power of this type of analysis. One option has been to investigate the mitochondrial coding region for polymorphisms that could differentiate between samples with identical control region haplotypes. The goal of this study has been to identify polymorphic coding region sites for development in a Pyrosequencing assay that …
Journey To The Scars: A White Trash Epic, J. Patrick Rader
Journey To The Scars: A White Trash Epic, J. Patrick Rader
Electronic Theses and Dissertations
Inspired by the work of writers Hunter S. Thompson and Tom Wolfe and motivated by celebrity prevaricator James Frey, Journey to the Scars: A White Trash Epic is a memoir that attempts to redefine the genre by applying the ideals and themes of gonzo and new journalism. The opening chapter, "The Diary of John Doe Frankenstein" tells the story of a pivotal event in the author's life. Immediately following this narrative of a near fatal motorcycle accident, the author/narrator's reliability is called into question and the remainder of the memoir is the story of the author's efforts to uncover the …
Nonlinear Optical Properties Of Organic Chromophores Calculated Within Time Dependent Density Functional Theory, Sergio Tafur
Nonlinear Optical Properties Of Organic Chromophores Calculated Within Time Dependent Density Functional Theory, Sergio Tafur
Electronic Theses and Dissertations
Time Dependent Density Functional Theory offers a good accuracy/computational cost ratio among different methods used to predict the electronic structure for molecules of practical interest. The Coupled Electronic Oscillator (CEO) formalism was recently shown to accurately predict Nonlinear Optical (NLO) properties of organic chromophores when combined with Time Dependent Density Functional Theory. Unfortunately, CEO does not lend itself easily to interpretation of the structure activity relationships of chromophores. On the other hand, the Sum Over States formalism in combination with semiempirical wavefunction methods has been used in the past for the design of simplified essential states models. These models can …
Catholic Students' Fatalism In Anticipation Of Transhuman Technologies, Stephen J. Lilley
Catholic Students' Fatalism In Anticipation Of Transhuman Technologies, Stephen J. Lilley
Sociology Faculty Publications
Findings are presented from a qualitative study in which participants from a Catholic university in New England, USA read a description of transhuman technologies (e.g., genetic engineering) and wrote lengthy responses indicating what they saw as the likely impact of these technologies on identity, society, and religion. In the subsequent content analysis, the responses were also examined for what Patrick Feng calls the “myth of technological determinism” — a sense that technological change is beyond human control. Most of the young men and women in the sample described negative impacts, identified a threat to religion and to their religious beliefs, …
2007 Schroeder Scholar In Residence Lecture The Role Of Science In Health Policy Decision-Making: The Case Of Emergency Contraception, Susan F. Wood
2007 Schroeder Scholar In Residence Lecture The Role Of Science In Health Policy Decision-Making: The Case Of Emergency Contraception, Susan F. Wood
Health Matrix: The Journal of Law-Medicine
No abstract provided.
Prevalence, Symptomatology, And Risk Factors For Depression Among High School Students In Saudi Arabia, Abdel-Rahman A. Asal, Moataz M. Abdel-Fattah
Prevalence, Symptomatology, And Risk Factors For Depression Among High School Students In Saudi Arabia, Abdel-Rahman A. Asal, Moataz M. Abdel-Fattah
Neurosciences
OBJECTIVE: To assess the prevalence and pattern of depression in a secondary school sample of Saudi Arabia adolescents. METHODS: Four hundred and ninety secondary school students, comprising 306 males (62.4%) and 184 females (37.6%), in the age group 16-20, were surveyed from January to May, 2005 in Taif, Saudi Arabia, using the Arabic Beck’s Depression Inventory (BDI) by a team consisting of a psychiatrist and psychologist. RESULTS: The prevalence of depression according to the Beck Depression Inventory (CBDI) (cut-off point: 19) was 110 (22.4%) for moderate (19-29), 36 (7.3%) for severe (30-40), and 18 (3.7%) as very severe (>40) …
The Uneasy Entente Between Legal Insanity And Mens Rea: Beyond Clark V. Arizona, Stephen J. Morse, Morris B. Hoffman
The Uneasy Entente Between Legal Insanity And Mens Rea: Beyond Clark V. Arizona, Stephen J. Morse, Morris B. Hoffman
Journal of Criminal Law and Criminology
No abstract provided.
Group Think: The Law Of Conspiracy And Collective Reason, Jens David Ohlin
Group Think: The Law Of Conspiracy And Collective Reason, Jens David Ohlin
Journal of Criminal Law and Criminology
No abstract provided.
The Fight For Post-Conviction Dna Testing Is Not Yet Over: An Analysis Of The Eight Remaining Holdout States And Suggestions For Strategies To Bring Vital Relief To The Wrongfully Convicted, Rachel Steinback
Journal of Criminal Law and Criminology
No abstract provided.
Proven Guilty: An Examination Of The Penalty-Free World Of Post-Conviction Dna Testing , Gwendolyn Carroll
Proven Guilty: An Examination Of The Penalty-Free World Of Post-Conviction Dna Testing , Gwendolyn Carroll
Journal of Criminal Law and Criminology
No abstract provided.
Using Groups Statistics To Sentence Individual Criminals: An Ethical And Statistical Critique Of The Virginia Risk Assessment Program , Brian Netter
Using Groups Statistics To Sentence Individual Criminals: An Ethical And Statistical Critique Of The Virginia Risk Assessment Program , Brian Netter
Journal of Criminal Law and Criminology
No abstract provided.