Digital Commons Network^™

Growth Behavior Of Fibroblasts Influenced By Small Changes In Polymer Structure, Saraswathi Doddi

Theses

Polymers are a promising class of biomaterials that can be engineered to meet specific end use requirements. The order and processing history of the polymer, which would alter the molecular orientation of the material could have a significant contribution towards cellular attachment and in turn, cell growth on the particular polymer. Surface properties of the material were considered to directly influence the properties of the adherent cells including cellular growth and reorganization. The present study is aimed at comparing cell growth on polyarylates with that of polylactic acid in their original state or by introducing small changes in the surface …

In Vitro Evaluation Of Human Mesenchymal Stem Cell Neural Differentiation On Tyrsine-Derived Polyarylates And Polycarbonates, Yee-Shuan Lee

Theses

Present spinal cord injury treatments cannot restore motor and sensory functions caused by the injury. These functions can return in the hopes of repairing the neural cells with a tissue engineered designed scaffold complex. The scaffold complex will include cells to repair and replace the damaged cells.

Mesenchymal stem cells (MSC) are multipotent adult stem ells that are capable of differentiating along several lineage pathways. Neural stem cell and MSC differentiating along the neural lineage bave been investigated both in vivo and in vitro depicting its feasibility. MSC for neural differentiation can be achieved by microenvironmental signaling. Substrate surface characteristics …

Effect Of Melatonin And Dopamine In Site Specific Phosphorylation Of Phosducin In Intact Retina, Arinzechukwu Okere Nkemdirim

Theses and Dissertations

Phosducin (Pdc) is a 28 kDa binding partner for the G protein beta gamma subunit dimer (G-beta-gamma) found abundantly in the photoreceptor cells of the retina and pineal gland. In the retina, light-dependent changes in cAMP and Ca2+ control the phosphorylation of Pdc at serine 73 and 54, respectively, which in turn controls the binding of Pdc to G protein beta gamma subunit dimer . G protein beta gamma subunit dimer binding has been proposed to facilitate light-driven transport of G protein beta gamma subunit dimer from the site of phototransduction in the outer segment of the photoreceptor cell to …

Science Assessment Of Deaf Students: Considerations And Implications Of State Accountability Measurements, Julie Mountain

Theses

As states increasingly require students to take standardized tests, it's crucial to examine the ability of such tests to accurately reflect actual student knowledge and skill within content domains. This project reviews the literature about assessment in general and written text features problematic to deaf readers. It seeks to analyze the content breadth and depth as well as the text readability and other linguistic features of Massachusetts' and New York's high school physics tests. Finally, it considers the use of tests with their potential linguistic bias for making decisions about student content mastery and potentially other high stakes decisions such …

The Hilltop 8-31-2005, Hilltop Staff

The Hilltop: 2000 - 2010

No abstract provided.

Automated Liquid Dispensing Pin For Dna Microarray Applications, Suganya Parthasarathy

Theses

This thesis describes the research and development of a new liquid dispensing/aspiring system that is capable of producing micro sized spots/droplets for molecular biology research and analysis. In particular, the application is focused on DNA microarray fabrication with the goals of smaller spot size, higher yield, more efficient usage of biological materials, and capability to handle high viscosity liquids. The new system is based on active sensing and control and it is part of a fully integrated robotic microarray system for genomic and proteomic applications. The prototype system handles water as well as thick liquids such as 100% glycerol and …

The George-Anne, Georgia Southern University

The George-Anne

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A Global Regulatory Role Of Gluconeogenic Genes In Escherichia Coli Revealed By Transcriptome Network Analysis, Katy Kao, Linh Tran, James Liao

Faculty Publications

In bacterial adaptation to the dynamic environment, metabolic genes are typically thought to be the executors, whereas global transcription regulators are regarded as the decision makers. Although the feedback from metabolic consequence is believed to be important, much less is understood. This work demonstrates that the gluconeogenic genes in Escherichia coli, ppsA, sfcA, and maeB, provide a feedback loop to the global regulator, cAMP receptor protein (CRP), in carbon source transition. Disruption of one of the gluconeogenic pathways has no phenotype in balanced growth, but causes a significant delay in the diauxic transition from glucose to acetate. To investigate the …

The Inkwell, Armstrong Atlantic State University

The Inkwell

No abstract provided.

Multifractal Analysis Of Heart Rate Variability Using Wavelet-Transform Modulus-Maxima Method, Chirag Jani

Theses

Physiological signals are complex and carry information of human health. Recent studies reveal that under normal conditions, the heart rate time series shows multifractal behavior. In contrast, HRV in the pathological state such as congestive heart failure exhibits more monofractal-like structure. Recent advances in the assessment of heart rate variability (HRV) uses a nonlinear dynamics approach. In this study, the main objective is to use the wavelet-transform modulus-maxima method for the multifractal analysis of the heart rate time senes.

The degree of the multifractality is defined by the singularities (a point in time series where a mathematical function is not …

Quantification Of Long-Range Power Law Correlations Among Healthy And Pathologic Subjects Using Detrended Fluctuation Analysis And Multifractal Detrended Fluctuation Analysis, Hardick Raval

Theses

The healthy heartbeat is traditionally thought to be regulated according to the classical principle of homeostasis whereby physiologic systems operate to reduce variability and achieve an equilibrium-like state. However recent studies reveal that under normal conditions, beat-to-beat fluctuation in heart rate display the kind of long-range correlations typically exhibited by the dynamical system far away from equilibrium. In contrast, heart rate time series from patients with severe congestive heart failure show a breakdown of this long-range correlation behavior. Two different non-linear dynamic methods namely Detrended Fluctuation Analysis (DFA) and Multifractal (MF) DFA are used for the quantification of this correlation …

Quantification Of Regularity In Rr-Interval Time Series Using Approximate Entropy, Sample Entropy, And Multi-Scale Entropy, Nirvish Shah

Theses

Heart rate variability (HRV) has proven to be a useful noninvasive tool to study the neuronal control of the heart. Recently, nonlinear dynamic methods based on chaos theory and fractal dynamics have been developed to uncover the nonlinear fluctuations in heart rate. Approximate Entropy (ApEn), Sample Entropy (SampEn) and Multi-scale Entropy (MSE) are measures based on chaos theory that quantify the regularity in time series. This study has been designed to examine the ability of these measures to distinguish the RR-interval time series of normal subjects (NSR) from subjects with congestive heart failure (CHF). The study was conducted on the …

Review Of "Multiple Pregnancy: Epidemiology, Gestation & Perinatal Outcome. Second Edition", Edward J. Hayes

Department of Obstetrics and Gynecology Faculty Papers

Book Review

Wet-Spinning And Thermal Analysis Of Collagen Fibers Incorporating Hydroxyapatite In Mixed And Coated Form, Sandesh Nathaniel Prakash

Theses

The composition of natural bone comprises of collagen (Cg) as organic phase which acts as a matrix and inorganic calcium phosphate, particularly hydroxyapatite (HA), which is used as reinforcement. This study involved the wet-spinning of collagen fibers incorporating HA in mixed and coated form. The weight percent ratio of collagen and HA in the composite fibers was 95 to 5 respectively. HA coating on pure collagen fibers was achieved by immersing them in HA solution formed by mixing HA in water. With Thermomechanical analysis, the highest breaking point temperature was obtained for the composite fibers and the least with pure …

Snp Auto-Calling Using Artificial Neural Networks, Damien Spivak

Theses

In recent years feedforward artificial neural networks (ANN) and their training algorithms have become an effective methodology for the construction of nonlinear systems that solve the statistical problem of classification. The ability of ANNs to solve this problem is highly germane to making progress in the refinement of DNA microarray analysis and techniques regarding this issue. This study attempts to deal with the classification of microarray data and the comparison and validation of simple feedforward ANNs in partitioning high dimensional data. In doing this the efficacy of using ANNs as a genotyping tool will be proven. Furthermore, it has been …

Hypoxia In The Gulf Of Mexico: A Legal And Practical Analysis, Bradford T. Mclane

ExpressO

Each year, a large area of the Gulf of Mexico is seasonally depleted of life-giving oxygen. Called hypoxia, the phenomenon threatens to bring about a collapse of the Gulf’s marine ecosystem. A voluntary regime is working to address this threat, and has set a year 2015 goal of considerably reducing the size of the Gulf hypoxic area to less than 5,000 square kilometers by 2015. Implementation of this goal will entail an estimated reduction in nitrogen loading to the Gulf of at least thirty percent.

This note analyzes the conceptual transformation of this voluntary regime into a regulatory one. Because …

The Limits Of Equality - Wishing For Discrimination?, Yifat Bitton

ExpressO

The Limits of Equality – Wishing for Discrimination? Yifat Bitton Abstract The article focuses on the different manner in which antidiscrimination regimes treat groups that have been discriminated-against de jure and de facto. The Article approaches the prerequisite of antidiscrimination laws that there be some past or on-going discrimination in a manner that diverges from the dominant view that discrimination is a purely destructive force. The central argument is that the way in which de jure, overt and blatant discrimination necessarily must create a coherent group identity recognized by law allows groups that are discriminated against in this manner to …

Rna Therapeutic, Pendekatan Baru Dalam Terapi Gen, Amarila Malik

Majalah Ilmu Kefarmasian

Some diseases, such as cancer, hereditary and genetic diseases, as well as viral infectious diseases, have been treated unsatisfied by the conventional therapy so far, and even more, by the gene therapy. Together with the pharmaceutical industries, researchers put their best effort to hunt some molecules that can be more favorable for such kind of therapy. After a pivotal study reported in May, 2001, it is certain that Ribonucleic acid (RNA) could effectively silence gene expression in mammalian cell line, so it was then proposed in 2004 the term RNA therapeutics. Antisense RNA therapy which came into the stage earlier …

Grand Valley Forum, Volume 030, Number 05, August 30, 2005, Grand Valley State University

2005-2006, Volume 30

Grand Valley Forum is Grand Valley State's faculty and staff newsletter, published from 1976 to the present.

Charge-Switch Nucleotides, John G. K. Williams, Gregory R. Bashford, Jiyan Chen, Dan Draney, Nara Narayanan, Bambi L. Reynolds, Pamela Sheaff

Biomedical Imaging and Biosignal Analysis Laboratory

The present invention provides compounds, methods and systems for sequencing nucleic acid using single molecule detection. Using labeled NPs that exhibit charge-switching behavior, single-molecule DNA sequencing in a microchannel sorting system is realized. In operation, sequencing products are detected enabling real-time sequencing as successive detectable moieties flow through a detection channel. By electrically sorting charged molecules, the cleaved product molecules are detected in isolation Without interference from unincorporated NPs and Without illuminating the polymerase-DNA complex.

Kemampuan Petugas Menggunakan Pedoman Evaluasi Pengelolaan Dan Pembiayaan Obat, Sriana Azis, M Hherman, Abdul Mun'im

Majalah Ilmu Kefarmasian

One important aspect of district public health service is the management and budgeting of drugs. Up to this time no indicators can be used yet as guidelines for evaluating drug management and budgeting effectiveness in a district in Indonesia. The objective of this study is to determine the effect of training managing drug staff on the evaluation capability increase and their knowledge. Training has been conducted twice in 2 provinces, 4 districts ex HP IV each, covering Dinas Kesehatan, GFK, 5 PHCs per district and 300 outpatients. There are 7 groups of indicator which include a total of 29 indicators. …

Human Mesenchymal Progenitor Cell, Beerelli Seshi

USF Patents

There is provided an isolated pluri-differentiated human mesenchymal progenitor cells (MPCs), a method for isolating and purifying human mesenchymal progenitor cells from Dexter-type cultures, and characterization of and uses, particularly therapeutic uses for such cells. Specifically, there is provided isolated MPCs which can be used for diagnostic purposes, to enhance the engraftment of hematopoietic progenitor cells, enhance bone marrow transplantation, or aid in the treatment or prevention of graft versus host disease.

Atypical Pkciota Contributes To Poor Prognosis Through Loss Of Apical-Basal Polarity And Cyclin E Overexpression In Ovarian Cancer, Astrid M. Eder, Xiaomei Sui, Daniel G. Rosen, Laura K. Nolden, Kwai Wa Cheng, John P. Lahad, Madhuri Kango-Singh, Karen H. Lu, Carla L. Warneke, Edward N. Atkinson, Isabelle Bedrosian, Khandan Keyomarsi, Wen-Lin Kuo, Joe W. Gray, Jerry C. P. Yin, Jinsong Liu, Georg Halder, Gordon B. Mills

Biology Faculty Publications

We show that atypical PKCι, which plays a critical role in the establishment and maintenance of epithelial cell polarity, is genomically amplified and overexpressed in serous epithelial ovarian cancers. Furthermore, PKCι protein is markedly increased or mislocalized in all serous ovarian cancers. An increased PKCι DNA copy number is associated with decreased progression-free survival in serous epithelial ovarian cancers. In a Drosophila in vivo epithelial tissue model, overexpression of persistently active atypical PKC results in defects in apical-basal polarity, increased Cyclin E protein expression, and increased proliferation. Similar to the Drosophila model, increased PKCι proteins levels are associated with …

The Hilltop 8-30-2005, Hilltop Staff

The Hilltop: 2000 - 2010

No abstract provided.

The George-Anne, Georgia Southern University

The George-Anne

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System And Method Enabling Simultaneous Investigation Of Sample With Two Beams Of Electromagnetic Radiation, John A. Woollam, Galen L. Pfeiffer, Daniel W. Thompson, Blaine D. Johs, Craig M. Herzinger

Department of Electrical and Computer Engineering: Faculty Publications

Disclosed are system and method for a characterizing system consisting of a fluid sample on a two-sided stage, utilizing data obtained by applying, from both sides thereof, beams of electromagnetic radiation to a fluid coated surface in a containing cell volume. The beams can have the same or different wavelength content and/or polarization state and can be applied at the same or different magnitude angles-of-incidence, and a third typically unpolarized beam can be applied at a normal angle-of-incidence.

System And Method Enabling Simultaneous Investigation Of Sample With Two Beams Of Electromagnetic Radation, John A. Woollam, Galen L. Pfeiffer, Daniel W. Thompson, Blaine D. Johs, Craig M. Herzinger

Department of Electrical and Computer Engineering: Faculty Publications

Disclosed are System and method for characterizing a System consisting of a fluid Sample on a two sided Stage, utilizing data obtained by applying, from both sides thereof, beams of electromagnetic radiation to a fluid coated Surface in a containing cell Volume. The beams can have the same or different wavelength content and/or polarization State, and can be applied at the same or different magnitude angles-of-incidence, and a third typically unpolarized beam can be applied at a normal angle-of-incidence.

Effects Of Long-Term Wastewater Application On Chemical Properties And Phosphorus Adsorption Capacity In Soils Of A Wastewater Land Treatment System, Cong Hu, Tian Zhang, Yong Huang, Mohamed F. Dahab, Rao Surampalli

Department of Civil and Environmental Engineering: Faculty Publications

The buildup of phosphorus (P) in the soil is a major factor limiting the operating life of a wastewater land treatment system. In this study, we evaluated changes of chemical properties, P profiles, and adsorption isotherms in the soils of a Muskegon wastewater land treatment system, which has received wastewater for ~30 years. It was found that the pH in the 15-cm topsoil increased from ~5-6 in 1973 to ~7.4-7.8 in 2003; a large amount of salt (e.g., Ca, Mg) in wastewater was adsorbed by the soil; the soil Al content (either exchangeable or oxalate extractable) decreased, while the oxalate-extractable …

Characterization Of The Yeast Ionome: A Genome-Wide Analysis Of Nutrient Mineral And Trace Element Homeostasis In Saccharomyces Cerevisiae, David J. Eide, Suzanne Clark, T . Murlidharan Nair, Mathias Gehl, Michael Gribskov, Mary Lou Guerinot, Jeffrey Harper

Dartmouth Scholarship

Nutrient minerals are essential yet potentially toxic, and homeostatic mechanisms are required to regulate their intracellular levels. We describe here a genome-wide screen for genes involved in the homeostasis of minerals in Saccharomyces cerevisiae. Using inductively coupled plasma-atomic emission spectroscopy (ICP-AES), we assayed 4,385 mutant strains for the accumulation of 13 elements (calcium, cobalt, copper, iron, potassium, magnesium, manganese, nickel, phosphorus, selenium, sodium, sulfur, and zinc). We refer to the resulting accumulation profile as the yeast 'ionome'.

08-30-05 (The Liberty Champion, Volume 23, Issue 1)

2005 -- 2006

No abstract provided.