Digital Commons Network^™

Separating Memory And Attention In Secondary Tasks, Anne Olsen

USF Tampa Graduate Theses and Dissertations

Memory and attention are intricately intertwined throughout life. How these processes interact and effect the encoding process shows to be just as complex and intricate. A recent study by Sahakyan and Malmberg (2018) revealed that when cognitive resources are strained during encoding, long-established retrieval patterns in both recall and recognition alter. These patterns found were suggested to be the result of an altered mechanism in strengthening processes that eliminated trace strengthening when cognitive resources were strained. This work investigates those claims through a direct replication of the original experiment (Experiment 1) as well as investigating the components of the secondary …

A Multi-Faceted Study Of Antibiotic-Resistant, Opportunistic Pathogens In Wastewater And Environmental Water, Jeanette Calarco

USF Tampa Graduate Theses and Dissertations

More than 2.8 million infections due to antibiotic-resistant bacteria occur annually in the United States, and more than 35,000 people die as a result. Domestic wastewater (sewage), which contains antibiotic-resistant bacteria and antibiotic resistance genes, is collected at treatment plants for removal of contaminants, e.g. pathogenic microorganisms and nutrients. While wastewater treatment is designed to remove antibiotic-resistant bacteria and other microorganisms through treatment and disinfection, some bacteria may escape treatment. Treated wastewater effluent is generally discharged into surface waters; however, some fraction of the effluent may be used as recycled water. Compromised wastewater collection infrastructure and sewage spills can disseminate …

Leveraging Dna Methylation Profiling And Microrna Sequencing To Characterize The Epigenomic Landscape Of Major Depressive Disorder Disease Trajectory, Jan Dahrendorff

USF Tampa Graduate Theses and Dissertations

Major depressive disorder (MDD) is a common and debilitating mental disorder associated with a significant disease burden and economic cost. Despite ample evidence documenting the efficacy of a diverse array of established MDD therapies, a large proportion of patients are inadequately responsive to initial treatment attempts, with first-line treatments leading to remission in only ~30% of the patients. Importantly, about a third of patients even fail to achieve symptom improvement after two or more antidepressant trials, resulting in what is commonly defined as treatment-resistant depression (TRD). The ability to identify which treatments are most likely to elicit a positive response …

Enhancing Nitrogen Removal In High Permeability Bioretention Media: The Combined Role Of Biochar, Wood Chips And Hydraulic Design, Joshelyn Guimaray

USF Tampa Graduate Theses and Dissertations

Urban stormwater runoff can transport high nutrient loads to receiving waters, leading toeutrophication, harmful algal blooms, and the formation of hypoxic zones. Bioretention systems are a form of green infrastructure designed to mitigate these impacts, but their application in dense, ultra-urban environments is often limited by large land area requirements. This has led to the adoption of high permeability media (HPM) to accommodate higher hydraulic loading rates (HLRs) and reduce the system footprint. However, conventional HPM systems often show inconsistent removal of dissolved nitrogen species due to short hydraulic retention times (HRTs), a lack of organic carbon, and insufficient anoxic …

Improving Coral Restoration Outcomes Through Novel Molecular Applications, Matthew H. Gamache

USF Tampa Graduate Theses and Dissertations

Coral reef ecosystems are declining globally from numerous stressors, including localized pollution, disease, and marine heatwaves induced by climate change. To preserve these ecosystems, it is critical to identify corals that can withstand stressors—primarily future marine heatwaves—and prioritize them for coral restoration efforts until these stressors are mitigated. Coral responses to thermal stress can be influenced by several partners, including mutualistic microalgae and a vast prokaryotic community. Here, I use advanced molecular techniques to examine which partners (coral host, algal symbiont, and microbial communities) are associated with coral health, how they contribute to holobiont physiology, and their potential to increase …

Exploring Perceived Parental Vulnerability In Pediatric Cancer Survivors: A Comparative Thematic Analysis Of English And Spanish-Speaking Families., Yusnavi Martinez Pineiro

USF Tampa Graduate Theses and Dissertations

This study examined how perceived parental vulnerability is expressed among parents of pediatric cancer survivors and explored potential differences between Spanish-speaking and English-speaking families. Data were drawn from an existing ongoing clinical trial in the IHOPE lab of the University of South Florida, who collaborates with over 10 pediatric cancer survivorship clinics across the country. The study used a total of 26 (13 Spanish-speaking and 13 English-speaking) structured interview transcripts from parent participants. The transcripts were the first session with the parent participant, the interventionist spoke the parent’s preferred language (English or Spanish), perceived parental vulnerability was one of six …

The Impact Of Storyline Curriculum On Student Engagement And Scientific Literacy: A Phenomenological Study Of High School Biology Teacher’S Experiences, Nicole Holman

USF Tampa Graduate Theses and Dissertations

This study looks at how using a storyline curriculum affects student engagement and scientific literacy in high school biology classes. It focuses on constructivist learning theory and explores the experiences of thirteen biology teachers who moved from traditional, teachercentered methods to student-centered, inquiry-based approaches. The teachers noticed a significant change in classroom participation; students took more ownership of their learning and showed increased interest and curiosity. As students engaged more in building their understanding, their critical thinking skills and ability to analyze and assess scientific data improved.

The results reveal that storyline curricula do more than help students grasp biology; …

Phenological Shifts In Nesting Behavior Of Leatherback Sea Turtles, Dermochelys Coriacea, Along Central And Southeastern Florida Beaches, Frank Jesse Dejohn

USF Tampa Graduate Theses and Dissertations

Climate change is altering environmental conditions worldwide, affecting the timing and success of critical life-history events in many species. The timing of leatherback sea turtle (Dermochelys coriacea) nesting behavior is closely linked to seasonal and local environmental conditions, yet multi-decadal shifts in nesting phenology associated with climate change remain poorly understood. Using long-term leatherback nesting and saturation tagging data (2000––2025), I first examined patterns in emergence dates, emergence times per night, and the number of nesting leatherbacks per night over this 26-year period. Moreover, I examined the impact of environmental covariates including day length, water temperature, precipitation, wind speed, barometric …

Disrupting Latina/X Media Representations: A Performance Art Installation Informed By Autoethnographic And Embodied Explorations, Sandra Cristina Roa

USF Tampa Graduate Theses and Dissertations

In this dissertation I use autoethnography and performance methods to create a performance art installation that examines and re-imagines the role of media representations in shaping how I as a Latina actively negotiates belonging and identifies this concept as always in a processes requiring negotiation. I argue belonging is influenced by how I see myself or don’t see myself in media representation and offer the counter-narrative as a way of asserting agency and disrupting marginalization. In this project, I tend to the tensions felt between being a community member, media maker, and qualitative researcher in order to disrupt media messages. …

The Ruling Relations Of Care: An Institutional Ethnography Of High-Risk Prenatal Care Experiences, Nicole L. Harris

USF Tampa Graduate Theses and Dissertations

Disrespect during pregnancy, labor, and delivery is common in the United States. The Centers for Disease Control and Prevention has estimated that approximately one in five women overall, and over 30% of Black women, report being ignored, dismissed, or verbally mistreated during their care. Public health efforts have primarily focused on individual-level interventions to improve patient-provider communication or reduce provider bias; however, these strategies have been critiqued for their limited scope and effectiveness in shifting outcomes. Instead, researchers and advocates have called for an examination of the structures that shape clinical encounters, including policies, protocols, workflows, and organizational culture. This …

Mosquito Classification And Explainability From Image Data Via Deep Learning Techniques, Farhat Binte Azam

USF Tampa Graduate Theses and Dissertations

According to the World Health Organization (WHO), mosquitoes are the deadliest animals on Earth, responsible for more human deaths annually than any other species. Mosquito-borne illnesses continue to pose severe risks to global health. In 2015 alone, there were an estimated 214 million malaria cases worldwide. Similarly, a 2016 report from the Centers for Disease Control and Prevention (CDC) revealed that Puerto Rico’s Department of Health received over 62,500 suspected cases of Zika, with 29,345 confirmed positive cases. In 2019, Southeast Asia experienced its worst dengue outbreak in recorded history. Of the approximately 4,500 mosquito species distributed across 34 genera, …

Using Rattlesnake Venom To Model Complex Trait Evolution Across Ecological And Evolutionary Scales, Samuel R. Hirst

USF Tampa Graduate Theses and Dissertations

A central challenge in evolutionary biology is understanding how genetic variation leads to phenotypicdiversity, particularly for complex traits that strongly influence fitness. Traits form the substrate of natural selection, but it is genes, that are inherited across generations. To fully understand the evolutionary process, we must therefore connect the molecular basis of trait variation with the evolutionary outcomes of such traits.

For relatively simple traits, the genotype–phenotype relationship is well understood. Classic systems, such as coat color in beach mice or toxin resistance in garter snakes, have provided effective examples of how relatively few loci underlie conspicuous adaptive differences across …

Cross-Linked Cellulose Ester/Linseed Oil Composites For Controlled Release Fertilizers, Ian Stark

USF Tampa Graduate Theses and Dissertations

Food scarcity is an ever-present concern for a growing population but can be mitigated through innovations in agriculture. Fertilizers are required to produce a sufficient amount of food, and while they contain key nutrients for crop growth, these nutrients can leech into the surrounding environment. Controlled release fertilizers slow the release of nutrients, encasing the prills in a semi permeable coating. Current coatings are non-biodegradable, causing microplastic soil contamination, which is very difficult to remove. Cellulose esters offer a compelling alternative, with an ability to cross-link into thin films that can be applied as coatings. The cellulose esterification was completed …

Dissecting The Intrinsic And Extrinsic Regulators Of Prostate Cancer Dormancy In The Bone Microenvironment, Mostafa M. Nasr

USF Tampa Graduate Theses and Dissertations

Metastatic disease is the primary driver of cancer-related mortality. In prostate cancer (PCa), patient survival decreases dramatically once the disease spreads to distant organs, with over 80% of metastases occurring in the bone. This dissemination is often followed by a clinically silent period of tumor dormancy that can last for years, creating a critical reservoir of cells poised for lethal relapse. A significant barrier to understanding and targeting this dormant phase has been the lack of robust experimental models. This thesis first addresses this challenge by establishing a novel stress-induced model of PCa dormancy, validated across multiple murine and human …

A Political Ecological Exploration Of The Caracol Road Project In Belize, Lorenzo A. Pantano

USF Tampa Graduate Theses and Dissertations

The Caracol Road Project is a new BZ$148,100,000 (US$74,050,000) road infrastructure development in the Cayo District of Belize and financed largely by foreign sovereign wealth funds. The areas adjacent to the road, broken into two phases include: communities, forest reserves, national parks, recreational areas, private lands and estates, and archaeological sites. This paper seeks to ask the questions: (i) For whom is the CRP being developed? (ii) For what is the CRP being developed? (iii) What visual evidence exists of sacrifice zones because of the production of the CRP? I sought to answer these questions through ethnographic observations, interviews, and …

Metabolic Insights Into Cyst Fluid Composition And The Role Of Taurine In Polycystic Kidney Disease, Vineetha Garimella

USF Tampa Graduate Theses and Dissertations

Polycystic kidney disease (PKD) is the most common genetic disorder affecting kidneys and the fourth leading cause of kidney failure. It occurs in two major forms, autosomal dominant PKD (ADPKD), and autosomal recessive PKD (ARPKD). ADPKD is typically adult-onset and caused by mutations in the PKD1 and PKD2 genes, and ARPKD generally manifests in utero and is caused by mutations in the PKHD1 gene. Despite the severity of the disease, only one FDA-approved drug, tolvaptan, is currently available for ADPKD and remains under clinical investigation for ARPKD. However, its use is limited by adverse side effects including polyuria, polydipsia, and …

Ai-Assisted Spectral Fingerprinting Of Mg-Doped Zno Tracer Phosphors: Tuning Photoluminescence Emission And Investigating Lifetime Via Sol-Gel Synthesis Parameters, Thomas M. Brandte

USF Tampa Graduate Theses and Dissertations

Plastic sorting remains a major driver of recycling costs, and existing infrared (IR) optical identification systems cannot determine material prominence or quality, nor reliably identify dark polymers [1]. This work explores a PVA-stabilized sol–gel and thermal synthesis route for magnesium-doped zinc oxide (Mg:ZnO) as a tunable photoluminescent tracer phosphor.

Lattice-defect control forms the foundation of phosphorescent emission tuning, enabling unique spectral fingerprints to be generated based on synthesis parameters—Mg molar ratio (Mg%), PVA mass (g), and calcination temperature (°C). Photoluminescence (PL) spectra (400–850 nm) were analyzed using Gaussian peak fitting and principal component analysis (PCA), reducing hundreds of spectral features …

Graphene Screen-Printed Electrode For Wound-Related Biosensing, Chanyapat Sriviroch

USF Tampa Graduate Theses and Dissertations

Monitoring wound healing is critical for improving patient outcomes, especially for chronic wounds, which often have complications like delayed healing and infections. Current methods for monitoring wound healing are invasive and costly. Also, these methods have a limited ability to provide real-time, continuous data. This study seeks to explore a graphene screen-printed electrode (SPE) electrochemical biosensor that is non-invasive and efficient to constantly monitor wound healing in real time. This research study focuses specifically on the electrochemical detection of the Human Neutrophil Elastase (HNE) and Secretory Leukocyte Protease Inhibitor (SLPI), which is one of the most abundant neutral proteinases in …

Dissolved Inorganic Carbon Metabolism In Bacteria, Jana Wieschollek

USF Tampa Graduate Theses and Dissertations

Dissolved inorganic carbon (DIC: CO2, HCO3⁻, CO32⁻) plays a central role in the metabolism of all living organisms. DIC is necessary for steps in essential biosynthetic processes, such as the synthesis of amino acids and nucleobases; in autotrophic bacteria, organic molecules are synthesized entirely from DIC. To ensure a steady supply of CO2 and HCO3 - needed for biosynthesis and/or autotrophy, organisms use bicarbonate transporters to increase the cytoplasmic concentration of this anion, and carbonic anhydrase (CA) to speed the interconversion of CO2 and HCO3 -, both in the cytoplasm and at the cell surface. This dissertation investigates DIC metabolism …

Oxygen Supply Capacity Of The Atlantic Horseshoe Crab,, Marty Sims

USF Tampa Graduate Theses and Dissertations

The horseshoe crab, Limulus polyphemus, inhabits shallow bays and estuaries where it encounters variable oxygen levels. Moreover, each year, the biomedical industry harvests hemolymph from hundreds of thousands of horseshoe crabs to use in bacterial contamination testing, yet the effects of bloodletting on their physiology are relatively unknown. This study aims to determine the oxygen supply capacity of Limulus polyphemus in relation to environmental hypoxia and biomedical bloodletting. Study animals were collected from the intertidal waters of Tampa Bay, Florida. Experiments included a control group that did not undergo substantial blood-volume reduction and a treatment group from which 10% of …

The Dark Side Of Synchrony: Investigating Affective Valence As A Moderator Of The Relationship Between Physiological Synchrony And Team Performance, Caroline N. Deal

USF Tampa Graduate Theses and Dissertations

Team processes are severely understudied, likely given their dynamic nature. Physiological synchrony (PS) may provide a solution, as PS provides a proximal, objective measure of team processes. PS is believed to develop through affect contagion, such that team members who catch each other’s emotions are more likely to physiologically synchronize. Extant research has found relationships between PS and performance; however, many questions remain regarding the nature, scope, and directionality of this relationship. I proposed that PS in the context of a positively valenced emotion would be beneficial to team performance, while PS characterized by a negatively valenced emotion would be …

Identifying B-Vitamin Requirements And Phycosphere Interactions For The Hab-Forming Dinoflagellate, Pyrodinium Bahamense Var. Bahamense, Lydia A. Ruggles

USF Tampa Graduate Theses and Dissertations

Harmful Algal Blooms (HAB) negatively affect economies, ecosystems, and public health through mass proliferation and toxin production. The HAB species, Pyrodinium bahamense var. bahamense, is a saxitoxin-producing dinoflagellate found in tropical and subtropical estuarine waters globally. In two Florida estuaries, Old Tampa Bay and the Indian River Lagoon, P. bahamense is a recurrent HAB species, and the exact timing and intensity of bloom peaks in these estuaries vary interannually. Factors that are suspected to contribute to blooms include temperature, residence times, nutrients, and salinity. However, many HAB dinoflagellates require vitamin B12 for growth and bloom development. Thus, B12 may influence …

Marigold-Silver Nanoparticles (Agnps) Complex: Characterization, Therapeutic Potential, And Future Approaches For Lung Injury Via Antioxidant And Anti-Inflammatory Effects, Navdeep Kaur

USF Tampa Graduate Theses and Dissertations

Chronic respiratory diseases (CRDs) and other lung conditions are a key cause of the problems facing global health, as they greatly increase morbidity, death, and the financial burden. In order to achieve the third Sustainable Development Goal of the United Nations, which is to reduce premature mortality from non-communicable illnesses by one-third by 2030, updated epidemiological statistics are crucial for directing prevention, control, and treatment measures. One of the major reasons of illness and death globally is chronic obstructive pulmonary disease (COPD). Lung damage and the emergence of CRDs, such as asthma, emphysema, COPD, and other respiratory disorders, are largely …

Formulation And Characterization Of Albumin Nanoparticles For Nsclc Photochemotherapy, Faisal Mohammad Shamim Khan

USF Tampa Graduate Theses and Dissertations

Non-small Cell Lung Cancer (NSCLC) is the most common type of lung cancer and is a leading cause of worldwide cancer-related death, which is a strong motivation to develop more efficacious approaches to its treatment. This thesis outlines the construction of folate-targeted bovine serum albumin (FA-BSA) nanoparticles co-loading Chlorin e6 (Ce6), a photodynamic therapy photosensitizer, and Evofosfamide (EVO), a hypoxia-activated prodrug, to enable synergistic photochemotherapy. The 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) is a water-soluble carbodiimide coupling reagent and used for amide bonds between carboxylic acid and amines. FA-BSA nanoparticles were prepared through a desolvation approach and stabilized through crosslinking by the carbodiimide reagent …

The Orange Glow In The Sunshine State: Three Visions Of Plato In Florida (1970-1990), Ryan Mcgahan

USF Tampa Graduate Theses and Dissertations

The PLATO network, a collection of mainframe computers, terminals, and educational software, has received an increasing amount of scholarly attention in the last decade as a social precursor to the modern internet. Existing histories have neglected to investigate the ways in which PLATO and its related business enterprise worked to accelerate the shift in university governance away from classical liberal ideas centering the public good and towards a more profit-centered neoliberal rationality. By tracing the rise of PLATO in Florida universities, this paper argues that PLATO aided and was aided by the shifting priorities of American universities in the 1970s …

Proteomic Characterization Of The Neuronal Bin1 Interactome In Homeostatic And Alzheimer’S Disease Mouse Brains, Joseph Mcmillan

USF Tampa Graduate Theses and Dissertations

The gene BIN1 is the second-largest genetic risk factor for late-onset Alzheimer’s disease (LOAD). It is expressed in neurons and glia in the brain as cell-type specific and ubiquitous isoforms. BIN1 is an adaptor protein that regulates membrane dynamics in many cell types. Previously, we reported that BIN1 predominantly localizes to presynaptic terminals in neurons and regulates presynaptic vesicular release. However, the function of neuronal BIN1 in relation to LOAD is not yet fully understood. A significant gap in the field is the unbiased characterization of neuronal BIN1-interacting proteins and proximal neighbors. To address this gap and help define the …

Approximating Data-Driven Combinatorial Search In The Feature Space: Pwfs, Mehmet Bugrahan Ayanoglu

USF Tampa Graduate Theses and Dissertations

This dissertation presents the development and application of a novel feature selection method, Probability Weighted Feature Selection (PWFS), designed to address key challenges in machine learning involving high-dimensional, noisy, or biased datasets. It is a comprehensive exploration of feature selection strategies to enhance the performance, interpretability, and practicality of machine learning models in both engineering systems and educational data mining. PWFS introduces a structured, probabilistic approach to feature selection that adaptively weights features based on their empirical contribution to model performance. This method not only accelerates convergence to near-optimal feature subsets but also enhances flexibility for ensemble learning by encouraging …

Analyses Of Morphological Effects Of Cryopreservation On Plasmodium Yoelii Sporozoites, Lauren J. Topley

USF Tampa Graduate Theses and Dissertations

Malaria is a vector-borne disease and is a major global public health threat caused by the genus Plasmodium. Of the five species that infected humans, Plasmodium vivax and Plasmodium falciparum contribute the highest rates of morbidity and mortality. Research efforts to develop antimalarial drugs and effective vaccines is imperative to the prevention of future cases. Plasmodium has three reproductive stages: sporogonic, liver, and blood. Sporozoites are injected into the human host from an infected female Anopheles mosquito, which travel to the liver to initiate liver stage malaria. Since few sporozoites are successful, the liver stage is a bottleneck of the …

Concentration And Composition Of Organic Contaminants In Blackfin Tuna, Yellowfin Tuna, And The Common Clubhook Squid In The Western Gulf Of Mexico, Lisa Rose-Mann

USF Tampa Graduate Theses and Dissertations

Cephalopods are an important component of pelagic food webs. They play ecological roles not only as predators but also as prey. However, their role as prey in the trophic transfer of organic contaminants to apex predators such as tunas in the Gulf of Mexico (GoM) is not well known. To investigate the importance of cephalopods in tuna diets, stomach samples were collected in February 2020 from tunas caught by rod and reel aboard the F/V Gulf Eagle near two large offshore floating oil/gas platforms in the northwestern GoM. Cephalopod samples were obtained from gut content analysis of 41 tunas caught …

Gender Representation Of Composers In Recommended Wind Band Literature And Prominent Performances, Devon Alexis Gray Porter

USF Tampa Graduate Theses and Dissertations

The purpose of this research was to evaluate gender representation in recommended literature in wind band repertoire and prominent performances. I collected data from music lists approved by the National Band Association (NBA) state chapters, programs from the Midwest Clinic and College Band Directors National Association, music vendor catalogues, the Teaching Music Through Performance in Band series, and Florida Bandmasters Association (FBA) Music Performance Assessment (MPA) past performances. I evaluated names of composers to determine the male-female ratios and quantities for recommended works from these sources. I conducted further analysis to determine if trends existed in the relationships between composer …