Digital Commons Network^™

Amplicon Sequencing Reveals Shifts In Microbial Community Composition In Response To Nutrient-Dense Piney Point Emergency Discharges, Christa A. Baranowski

USF Tampa Graduate Theses and Dissertations

Due to a leak in the lining of a phosphogypsum pond at the former Piney Point phosphate-processing facility in Tampa Bay (Florida, U.S.A.), experienced a large influx of nutrient-dense waters in the early summer of 2021. As an emergency response effort, water samples were collected by researchers at the University of South Florida, College of Marine Science (USF CMS), covering the lower and middle regions of Tampa Bay (TB), over the span of three months (April – June). Illumina amplicon sequencing was performed on DNA from water samples, targeting bacterial and eukaryotic ribosomal rRNA genes, and processed into Amplicon Sequence …

The Emotional Construction Of Religious Experience: Senitmentalism And Moral Motivations, Yvonne Candelario

USF Tampa Graduate Theses and Dissertations

This paper addresses the growing prevalence of reported religious experiences across diverse religious and spiritual traditions, alongside the persistent absence of such reports among many adherents within the same communities. This asymmetry poses a challenge for both supernaturalist and reductively skeptical accounts of religious experience. I develop a naturalistic constructivist framework to explain why some individuals choose to interpret and report some human experiences as religious experiences or report having religious experiences at all, while others do not. Drawing on a post-modern revision of moral theory, an account of constructive sentimentalism, I argue that religious experience is shaped by socially …

Dual-Modal Tumor Imaging Using Functionalized Iron-Oxide Nanoparticles For Mri And Fluorescence, Kevin Secades

USF Tampa Graduate Theses and Dissertations

Accurate tumor imaging is essential for early cancer detection and treatment monitoring [1]. Technetium-99m (Tc-99m) is a commonly used radioisotope for medical imaging, but its reliance on gamma-ray emission poses challenges related to safety, accessibility, and regulatory constraints[2-5]. This research proposes the development of a dual-modal imaging approach by functionalizing iron-oxide nanoparticles (IONPs) with the fluorescent chromophore Cy5, enabling both MRI contrast enhancement and optical imaging for improved tumor specificity. IONPs provide strong T2-weighted MRI contrast [6], while conjugated near-infrared (NIR) chromophores allow for high-resolution fluorescence imaging [7], making this proposed system adaptable for both in vitro and in vivo …

Analysis Of The Yeast Surface Display Platform As A Tool For Tyrosine Kinase Phosphorylation Assays, Alexandra Maritza Tang

USF Tampa Graduate Theses and Dissertations

Developing and optimizing platforms to study complex protein-protein interactions is important to maintain fidelity of the modeled activity. Yeast surface display has been used as a high-throughput platform to model substrate phosphorylation by tyrosine kinases, a key enzymatic process that is essential for most cell signaling pathways. However, existing characteristics of the yeast surface display platform limit the ease-of-use, reproducibility, and feasibility of screening enzymatic modification processes like tyrosine phosphorylation. The cytoplasmic domain of T cell costimulatory receptor CD28 and lymphocyte kinase (LCK) were used as a model enzyme-substrate pair to showcase molecular cloning issues that can hinder reproducibility and …

Aging In The Shadows: Szekler Cemeteries And The Case For Recognizing Elderly Populations In Bioarchaeology, Katie M. Mcgrath

USF Tampa Graduate Theses and Dissertations

The study of aging in past populations lies at the intersection of bioarchaeology and paleodemography, offering insight into both biological processes and lived social experience. Despite this potential, older adults are often rendered “invisible” in skeletal assemblages due to methodological and cultural biases. This thesis addresses this gap through a rare, community-based study of aging among the Székely population of Transylvania. By integrating osteological analysis of individuals (11th–19th centuries) from the archaeological Papdomb church cemetery with demographic data derived from the modern cemetery headstones (ca.1850–present), this research examines patterns of aging, longevity, and elder visibility across a culturally and spatially …

Behind The Bar: How Bartenders And Regulars Co-Produce Guardianship In Neighborhood Bars, Jack I. Wexler

USF Tampa Graduate Theses and Dissertations

Although bars are commonly conceptualized as sites of disorder and violence, they are also spaces where social order is routinely produced and maintained. Bars are frequent targets of crime prevention efforts because they tend to concentrate aggression, disorder, and crime; yet little is known about how barroom actors—owners, bartenders, and regular patrons—actively maintain order. Explanations typically emphasize (a) physical features of bars (layout, lighting, crowding, door policies), (b) individual and situational predictors (clientele mix, intoxication levels), and (c) the actions of formal controllers—owners, bartenders, and bouncers. I consider an additional, and overlooked informal controller: the bar regular. This qualitative, exploratory …

J.K. Rowling’S Loss Of Control: Authorial Anxieties Within The Actor Network, Casey Sausville

USF Tampa Graduate Theses and Dissertations

This thesis investigates how traditional ideas of authorship are contradicted in the 21st century as authorial power is distributed through an actor network. In this case study, I apply Bruno Latour’s Actor Network Theory (ANT) to the authorship of the fantasy series Harry Potter, in which J.K. Rowling, Warner Bros, and the fandom are all among the co-creators. In doing so, I find that when power has been exercised by other actors in the network, this has in multiple cases been experienced by Rowling as a threat to her security as an author, a woman, and a mother. This is …

Youmatter: A Nursing Workforce Study, Stacy L. Pryor

USF Tampa Graduate Theses and Dissertations

The sustainability and health of the nursing profession are immediate and pressing concerns. Despite decades of research, nurses continue to struggle with high levels of stress and burnout, raising concerns about patient safety and error risk. One in 10 patients is estimated to experience an adverse patient event, with half being preventable, underscoring the need to understand the factors that affect stress among nurses and the subsequent risk of adverse patient events. This dissertation has the following aims: 1) to evaluate whether cognitive failure risk that could lead to an adverse patient event was associated with subjective measures of stress, …

Targeting Cancer With T Cells: Preclinical And In Silico Insights To Improve Immunotherapies, Nina Obertopp

USF Tampa Graduate Theses and Dissertations

T-cell–based immunotherapies are often constrained by limited trafficking and persistence in tumors and by functional exhaustion under chronic antigen exposure. This dissertation addresses these barriers through two distinct strategies: (i) preclinical studies testing how radiation therapy modulates tumor-infiltrating lymphocytes (TIL) therapy, and (ii) agent based modeling (ABM) to optimize treatment day treatment-free intervals (TFI) in T-cell engager (TCE) therapy. (i) In a human papillomavirus–positive (HPV⁺) head-and-neck cancer (HNC) model, a single local-dose of RT (8 Gy) delivered five days before tumor harvest increased the success and functional quality of ex vivo TIL outgrowth from tumor fragments, enriching polyfunctional CD8⁺ subsets. …

Lymphatic Malformations With Activating Kras Mutations Impair Lymphatic Valve Development Through Matrix Metalloproteinases, Diandra M. Mastrogiacomo

USF Tampa Graduate Theses and Dissertations

Lymphatic malformations (LMs) are lesions that can be due to inherited or somatic mutations, and they lead to a defective lymphatic vasculature. Activating KRAS mutations have been identified recently in LM patients with lymphedema, chylous ascites, or life-threatening chylothorax. In a LM mouse model, KRAS mutations are associated with a loss of lymphatic valves, which has been proposed to cause chylothorax via retrograde lymph flow into the pleural space. However, the mechanisms underlying the loss of lymphatic valves are unknown. To investigate the mechanisms leading to valve loss, we combined the lymphatic-specific and tamoxifen-inducible Flt4CreERT2 with Kras-loxP-stop-loxP-G12D (Kras+/G12D) mice and …

The Role Of Fkbp51 In The Regulation Of Reproductive Aging And Prenatal Stress-Impaired Ovarian Function And Development, Monica C. Moore

USF Tampa Graduate Theses and Dissertations

FK506-binding protein 51 (FKBP51) is an immunophilin that acts as a co-chaperone with heat shock protein 90 that negatively regulates transcriptional activity of glucocorticoid receptor (GR) and progesterone receptor (PR), and FKBP51 levels are increased by stress conditions. Our previous studies demonstrate that Fkbp5 gene depletion was protective against maternal re-strained stress-induced preterm birth (PTB), fetal growth restriction (FGR), and maternal stress-induced age-related decline in live births. We hypothesize that FKBP51 contributes to impaired ovarian development and folliculogenesis induced by prenatal stress (PNS). Timed-pregnant Fkbp5+/+ (wild-type) and Fkbp5−/− (knockout) mice were randomly assigned to either the undis-turbed (nonstress) or PNS …

Navigating (M)Otherhood: Perception, Practice, & Policy Of Adolescent Pregnancy In The North Coast Of Perú, Mahir Rahman

USF Tampa Graduate Theses and Dissertations

“It’s a vicious circle”, the obstetricians of Huanchaco repeated time and time again after discussing a recently pregnant 13-year-old girl who had just moved to the North Coast of Perú. Adolescent pregnancy is an extremely stigmatized topic in global maternal and child health discourse. Pregnant adolescents are subject to educational policy, clinical protocol, and community practice. While global scholarship emphasizes adolescent pregnancy prevention, limited scholarship focuses on the conditions in which adolescent pregnancy is perceived, discussed, and managed. More importantly, these perspectives are even less documented amongst the North Coast of Perú.

This thesis serves as an ethnographic exploration of …

From The Tropics To The Poles: Chemical Investigation Of Marine Invertebrates Through Elucidation And Synthesis, Benjamin Reed Smith

USF Tampa Graduate Theses and Dissertations

Society’s perpetual need for new disease treatments continuously leans on the shouldersof willing researchers. Even in modern day, many described diseases lack a cure or a symptomatic treatment; worse yet, the necessity for new drugs is amplified with rises in drug- resistant microbial infections, such as methicillin-resistant Staphylococcus aureus (MRSA). Historically, remedies and disease treatments have been rooted in the medicinal properties of plants and animals; naturally derived analgesics, like milk of the poppy or willow bark tea, date back thousands of years. With the developments of modern medicine, drug discovery, and natural product researchers found a wealth of potential …

Emotion Differentiation As A Moderator Of The Relationship Between Affect And Alcohol Use Among College Students: An Ecological Momentary Assessment, Gaelle El Helou

USF Tampa Graduate Theses and Dissertations

Alcohol use remains prevalent among young adults and particularly college students. Research shows a link between affective states and alcohol consumption, although recent findings appear to be unequivocal on the effect of negative and positive emotions on college drinking. While emotion differentiation (ED) has been associated with alcohol use, previous research has focused mostly on clinical treatment adult populations and / or negative emotions, focusing less on the role that positive emotions play in drinking, particularly among college students. The current study explored the moderating role of negative and positive emotion differentiation (NED / PED) in the association between negative …

Eye Of The Beholder: Exploring Correlates Of Subjective Cognition In Older Adults With And Without Insomnia, Amy N. Costa

USF Tampa Graduate Theses and Dissertations

It is unclear how subjective cognition is related to current objective cognitive functioning in older adults, but subjective cognitive complaints are a risk factor for future cognitive decline. A lack of awareness of even subtle changes in objective cognitive performance could lead to missed opportunities for early intervention. Given past work and known associations between sleep, cardiac autonomic functioning, and cognition (both subjective and objective), it is plausible to hypothesize these factors may influence subjective cognition. Understanding how and when the subjective and objective cognition relationship diverges may inform clinicians on how to best assess cognitive functioning and factors (i.e., …

An Autoethnographic And Relational Exploration Of Identity Negotiation Within Romantic Relationships Between Nonbinary Partners And Cisgender Women, Maddison L. Russell

USF Tampa Graduate Theses and Dissertations

This dissertation explores how trans and cisgender partners negotiate gender identity, care, and relational well-being through an autoethnographic examination of everyday communication. The study draws from eighteen personal narratives across family, academic, and romantic contexts to trace how gender-based microaggressions and cisnormative expectations become embedded in ordinary talk, gestures, and silences, shaping identity development and relational stability. At its core, this work argues that relationality emerges as a pervasive and constitutive condition that shapes how affirmation, misrecognition, and belonging are experienced within trans/cis relationships and across time. Through examining moments of misunderstanding, minimization, and avoidance, the study reveals how microaggressions …

Microplastic Contamination In Longfin Squid (Doryteuthis Pealeii) From The Florida Gulf Coast: Evidence Of Marine Based Pollution, Jordan Kleinschmidt Dilena

USF Tampa Graduate Theses and Dissertations

Microplastic contamination is a pervasive global issue, yet its impact on ecologically significant cephalopod populations remains poorly understood. This study quantified microplastic prevalence and distribution in longfin squid (Doryteuthis pealeii), a key trophic link in the Gulf of Mexico. Following hydrogen peroxide digestion of digestive tract and mantle tissues, microplastics were isolated via vacuum filtration and characterized using microscopy. Fourier-transform infrared (FTIR) spectroscopy confirmed polymer identities. Microplastic contamination was exceptionally high, with 96.4% of individuals containing particles (mean = 36.6 ± 20.0 particles per individual, range = 0–94, n = 110 squid). From 4,072 total particles analyzed, fragments dominated (98.3%), …

Considering Context In Latent Models Of Externalizing: Measurement Invariance By Environmental And Systemic Disadvantage In System-Involved Youth, Kendall D. Smith

USF Tampa Graduate Theses and Dissertations

Externalizing forms of psychopathology are said to result from a general liability towards impulsive and disruptive behaviors, including substance use, conduct problems, aggression, and antisocial behavior (Krueger et al., 2021). While the field often focuses on dispositional explanations of externalizing behaviors, there is a notable lack of attention to the contextual factors that contribute to these behaviors, beyond immediate environmental influences. This study uses Ecological Systems Theory (Bronfenbrenner, 2005) as a framework to address such gaps in the context of behavioral indicators of the latent externalizing (EXT) construct, an analysis with implications for understanding racial/ethnic and socioeconomic disparities. The present …

Ifnγ-Driven Skewing Towards Th1 Over Th17 Differentiation Underlies Crs And Neutropenia In Car-T Therapy, Payal Goala

USF Tampa Graduate Theses and Dissertations

CAR-T therapy has improved overall and complete response rates, andprolonged progression free survival of patients with relapsed/refractory hematological malignancies. The short-term and long-term impacts of anti-CD19 CAR-T products on the immune landscape of B cell malignancies has garnered considerable attention in recent years. Most notably, a subset of patients experience high-grade cytokine toxicities, including cytokine release syndrome and immune cell-associated hematologic toxicity that manifest as prolonged cytopenia. Whereas etiology of CRS is more well established, the mechanisms leading to CAR-T associated cytopenia remains elusive. Rejeski et. al. showed that severe cytopenia in patients post CAR-T treatment led to frequent infections …

The Paleoecology Of The Lower Chincha Valley, Peru Using Pollen, Phytolith, And Geomorphological Analyses, Christine L. Bergmann

USF Tampa Graduate Theses and Dissertations

This research is a part of a larger project, undertaken by the Programa Arqueológico Chincha (i.e., PACH). The focus of the project centered on the societal, economic, political, and environmental mechanisms involved in the advent of social complexity on the south coast of Peru, specifically in the Chincha Valley. PACH comprehensively studied archaeological sites in the upper, middle, and lower valleys. One of the sites studied was Pozuelo. Pozuelo is a type site located in the lower Chincha Valley (and the focus of this dissertation). Pozuelo possesses the earliest ceramics, domestic activities, and mound structures in the Chincha Valley. Furthermore, …

Self-Determination And Its Role In The Foster Care Environment: A Three-Part Investigation, John Martin

USF Tampa Graduate Theses and Dissertations

Youth have multiple pathways to adulthood and for some of those youth that journey takes them through the foster care system either temporarily or until emancipation. A vital part of the foster care system is to protect and care for children in a variety of circumstances including unsafe and traumatic experiences. However, a history of foster care has been tied to certain risks like substance use. The focus of this dissertation is to investigate the strength of the relationship between self-determination and substance use through the lens of Self-Determination Theory (SDT) in foster care in three phases. In Study I …

A Bioarchaeological Study Of The Individuals From The Városfalva Burial Mound In Transylvania, Romania (15th-19th Century), Vanessa C. Reeves

USF Tampa Graduate Theses and Dissertations

This study explores the lives and deaths of the Szekler people recovered from a burial mound in the Transylvanian village of Városfalva. The Transylvanian plateau is located in present day Romania, and is protected on the east and the south by the Carpathian Mountains. The Szekler people arrived in this area around 1000 CE, and were a nomadic group originating in the Asian Steppes region who later intermarried with various migratory groups as they moved across the Eurasian plains. The Szeklers eventually banded together with the proto-Hungarian Magyar peoples and settled in the Carpathian Basin, the easternmost frontier of the …

“For Haitian Culture, Being Older [Is] A Blessing”: Perceptions Of Aging, Elderhood, And Dementia In A Haitian Born Sample, Melissa K. Eustache

USF Tampa Graduate Theses and Dissertations

This thesis investigates how first-generation Haitian immigrants in the United States understand and interpret dementia within the intersecting contexts of culture, migration, and biomedicine. While dementia is constructed as a pathological “loss of self” and a public health epidemic, in American biomedical and popular discourse, this study examines how Haitian-born individuals—socialized within a non-Western cultural and spiritual framework—make sense of cognitive decline and impairment in ways that diverge from Western biomedical narratives. Guided by theoretical orientations from medical anthropology, including Mark Nichter’s idioms of distress, Margaret Lock’s local biologies, and neuroanthropology, this research explores how cultural models of illness, aging, …

Reducing Data Requirements In Polymer Science: Deep Neural Networks For Predicting Surface Tension Of Copolymer Compatibilizers, Md Mushfiqul Islam

USF Tampa Graduate Theses and Dissertations

In polymer chemistry, compatibilization involves adding a substance often a block or graft copolymerto stabilize polymer blends that would otherwise not mix well, leading to rough structures and weak me- chanical properties. Compatibilizers improve miscibility and reduce interfacial tension, which is critical for applications such as mixed-waste polymer recycling. Sequence-controlled polymers offer unique potential by combining the tunable chemistry of synthetic polymers with the precise, function-driven design of biological macromolecules, but unlike proteins, they lack large, evolution-shaped datasets to guide discovery. This research develops a deep learning framework to predict the surface tension of sequence-controlled copolymer compatibilizers across varying concentrations. …

Sound Practices In Human Resources: A Critical Analysis Of How Ai Applicant Screening Tools Are Used With Deaf Individuals In The Hospitality And Tourism Sector, Sherry L. Gaynor

USF Tampa Graduate Theses and Dissertations

This study explores the intersection of Career and Workforce Education, Deaf Education, and artificial intelligence (AI) technologies used in hiring practices. The purpose of this research is to identify potential barriers to equitable employment for Deaf and Hard-of-Hearing (DHH) individuals and to propose effective methods for evaluating and assessing AI and machine learning applications utilized in recruitment and selection processes. Although AI is increasingly embedded in human resource management (HRM), there remains a significant lack of research addressing its use with special populations. In particular, no prior studies have examined how voice recognition software, facial analysis, psychometric testing, and virtual …

Development And Initial Validation Of An Instrument To Measure Teachers’ Socioscientific Issues Instruction Perceptions, Michael Edward Mitchell

USF Tampa Graduate Theses and Dissertations

Little is known about how high school science teachers perceive Socioscientific Issues (SSI) on a broad scale, including what factors shape their views of a pedagogical shift to adopt SSI. To address this critical gap, I constructed the Teachers’ Socioscientific Issues (SSI) Perceptions Survey (TSPS), the first instrument specifically designed and initially validated to measure teacher perceptions of SSI integration. This study presents the development and validation of the TSPS, offering robust evidence for its reliability, fairness, and construct validity.

Survey development adhered to gold-standard psychometric protocols (Bandalos, 2018; Crocker & Algina, 1986; DeVellis, 2012; McCoach et al., 2013) and …

Spatio-Temporal Characterization Of Trace Metals Across The West Florida Shelf And During The Decline Of The North Atlantic Spring Bloom, Delfina Paola Navarro-Estrada

USF Tampa Graduate Theses and Dissertations

Phytoplankton in the marine environment require the trace metals manganese (Mn), iron (Fe), cobalt (Co), nickel (Ni), copper (Cu), zinc (Zn), and cadmium (Cd) to catalyze cellular processes driving photosynthesis, including macronutrient (nitrogen, phosphorus, and carbon) acquisition, light harvesting, and protection from oxidative stress. The high biological demand for these elements, coupled with their low concentrations in the ocean, can limit primary production and shape community composition in marine ecosystems, highlighting the need to understand the drivers behind trace metal distributions and their interplay with phytoplankton growth across biogeochemical regimes. This dissertation characterizes the spatial and temporal distributions of dissolved …

Parameterizing Ion–Lipid Interactions From Local Clusters To Reproduce Experimental Bulk Properties Of Lipid Bilayers, W. Matthew Saunders

USF Tampa Graduate Theses and Dissertations

Phospholipids are the main component of cell membranes, providing a delimiting boundary for cells from their environments or from their organelles from the cytosol. Thiscompartmentalization allows cells to maintain quite different solutions within their cells and organelles, including different mixtures dissolved ions. Consequently, these membranes are constantly flanked by complex mixtures of salts at varied concentrations. To study membrane structure, one cannot neglect the contribution of the salts in the local solution. Ions perturb bilayer structure via specific interaction with the phospholipids, primarily with the headgroups. Experimental studies of these systems are somewhat at odds — scattering experiments with different …

Communicating The Truth-Telling And Space-Making Of Black Women In Higher Education: A Trap Feminist Study, Chazman Deira Mckoy

USF Tampa Graduate Theses and Dissertations

This dissertation adopts a “for Black women, by Black women” approach to examine how Black women’s lived experiences and performances of racialized gender become communicative acts of agency as they navigate the intersecting challenges of race and gender within pedagogical contexts.

Grounded in Black Feminist Pedagogy and expanded through Trap Feminism, this study celebrates the complexity of racialized gender performances and theorizes them as intentional communicative acts of resistance. These acts not only confront dominant ideologies but also create ongoing spaces for truth-telling, spaces where Black women can reframe how their stories, labor, and brilliance are recognized in academic contexts …

Investigating Whether Giving Or Receiving Support Impacts Caregivers Who Were Maltreated Or Not Maltreated Differently, Tara D. Matta-Singh

USF Tampa Graduate Theses and Dissertations

Caregivers can experience higher levels of stress, anxiety and depression compared to non-caregivers. However, a growing body of literature is beginning to show that while caregiving may be stressful, there may also be benefits to the caregiver, such as feeling closer to the care recipient, a sense of purpose or personal growth. Further, research now demonstrates that giving support to others can be more beneficial than receiving support from others in terms of mental health and even mortality. While these are important advances in the literature, understanding the impacts of giving and receiving support on individuals who were maltreated by …