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Testing The Impact Of Sexual Minority Stress On Endorsement Of Prospective Behaviors Related To Borderline Personality Disorder Among Lesbian, Gay, Bisexual, And Queer Adults, E. Elisa Carsten

USF Tampa Graduate Theses and Dissertations

Sexual minority individuals face increased risk for numerous forms of psychopathology compared to heterosexual individuals, including borderline personality disorder (BPD). Previous investigation into the causes of these mental health disparities highlights several sexual minority-specific factors that may render sexual minority individuals uniquely vulnerable to meeting criteria for a BPD diagnosis, including chronic exposure to minority stressors, such as sexual orientation-based discrimination and stigmatizing beliefs. Some scholars have argued that the relevance of these minority-specific stressful experiences hampers the utility of a BPD diagnosis among members of this historically marginalized population. The present study experimentally tested the influence of minority stressors …

Assessment Of Artificial Oyster Reef Health Based On Water Quality, Oyster Recruitment, And Perkinsosis Infection In Tampa Bay, Christian J. Goldman

USF Tampa Graduate Theses and Dissertations

Providing nursey habitat, regulating nutrients, stabilizing shorelines, increasing biological diversity, improving water quality, and creating three-dimensional environments are some of the ecosystem services of oyster reefs. Oyster reefs are threatened by overharvesting, changes in water quality, pollution, and disease. The Eastern oyster, Crassostrea virginica, is the primary oyster species along Florida’s Gulf coast. This study aimed to evaluate the relationship between multiple parameters on artificial reefs and how they influence the health of these reefs. Here, young (less than five years old) artificial oyster reefs were monitored for one year across three sites in Tampa Bay, FL: Lassing Park (LAS), …

Exploring Menstrual Cycle Experiences And Well-Being In Young Women With Type 1 Diabetes, Yolanda Rotzinger Ballesteros

USF Tampa Graduate Theses and Dissertations

Young women with type 1 diabetes (T1D) navigate complex physical, emotional, and social challenges, being at a higher risk for mental health concerns (e.g., depression), as well as physical (e.g., fatigue) and cognitive (e.g., focus) symptoms related to the management of their chronic health condition (Dantzer et al., 2003; Northam et al., 2005). About one-third of young women with T1D experience some form of menstrual dysfunction (Griffin et al., 1994; Schweiger et al., 2011), which is associated with lower quality of life across several domains, including the educational setting (Knox et al., 2015). Nevertheless, there is a gap in the …

Quantitative Determination Of Nano Delivered Kras Sirna, Axel S. Deraspe

USF Tampa Graduate Theses and Dissertations

This study focuses on the development and validation of analytical methods for the detection of therapeutic KRAS-targeting siRNA in biological systems. Physicochemical characterization showed that p5RHH-formulated siRNA nanoparticles exhibit a controlled nanoscale size distribution, moderate polydispersity, and a negative zeta potential, suggesting colloidal stability and potential suitability for cellular uptake. Scanning electron microscopy confirmed a predominantly spherical morphology with a relatively uniform size distribution, consistent with dynamic light scattering measurements.

To enable accurate siRNA quantification, stem–loop reverse transcription (SL-RT) coupled with SYBR Green–based quantitative Polymerase Chain Reaction (qPCR) was optimized. Key parameters, including a primer concentration of 100 nM and …

The Long-Term Biological Toll Of Adverse Childhood Experiences And The Role Of Psychological Resilience, Jill Krier Desch

USF Tampa Graduate Theses and Dissertations

Adverse childhood experiences (ACEs) are preventable, potentially traumatic events that occur before age 18 such as physical, sexual, and emotional abuse, neglect, and household instability and are associated with the leading causes of adult morbidity and mortality in the United States. The mechanisms underlying these associations are not yet fully understood, but the link between ACEs, chronic stress, and allostatic load is one potential pathway. Although ACEs are associated with the leading causes of adult morbidity and mortality, many individuals continue to lead healthy lives through adulthood despite exposure to ACEs. This dissertation used data from Waves I – V …

Situating Stormwater Infrastructure Through Collaborative Design: Insights From A University-Community Partnership, Serena A. Echols

USF Tampa Graduate Theses and Dissertations

Stormwater ponds help address flooding and partially mitigate nutrient pollution while sometimes serving as a central landscape element in community parks. In underserved urban communities, however, stormwater ponds are sometimes neglected and underutilized, leading to public health and environmental justice challenges. Our research in Tampa, Florida, seeks to develop pathways for residents and other stakeholders to collaboratively co-design infrastructure interventions and pondscape redevelopment for neglected stormwater ponds. Drawing on ethnographic and participatory methods as well as environmental assessment and modeling, we are working with residents of one pond community on cleanup and redevelopment strategies. Results reveal that balancing local and …

Polygenic Risk Scores, Sex, And Socioeconomic Factors As Predictors Of Age Of Onset For Major Depressive Disorder, Kaitlyn M. Nowak

USF Tampa Graduate Theses and Dissertations

Major Depressive Disorder (MDD) is a heterogeneous psychiatric condition influenced by genetic, sociodemographic, and environmental factors, yet the extent to which these factors jointly shape the timing of disease onset remains unclear. This study evaluated the effects of polygenic risk scores (PRS), sex, and socioeconomic status (SES) on age of onset for MDD across two independent cohorts: the Pharmacogenomics Research Network Antidepressant Medication Pharmacogenomics Study (PGRN-AMPS) (N = 486) and the All of Us Research Program (N = 8,014). Analyses were restricted to individuals of European genetic ancestry, and PRS were constructed using both European-ancestry (EA) and multi-ancestry (MA) genome-wide …

The Architecture Of Chemical Understanding: How Representation Selection, Ordering, And Feature Salience Shape Students' Conceptions In Chemistry, Isaiah Nelsen

USF Tampa Graduate Theses and Dissertations

Chemical representations play a central role in how students learn and reason aboutchemistry. While representations are routinely used in chemistry classrooms, students often struggle to extract relevant information, connect representations to underlying chemical principles, and apply this information to problem-solving. This dissertation investigates the role of representations in supporting students’ conceptual understanding and reasoning across general and organic chemistry through three qualitative studies.

The first study in this work explores how variation in chemical representation influences how students understand foundational chemistry principles. This study sought to characterize how second semester general chemistry students approach dipole–dipole interaction tasks with four distinct …

Combating Β-Lactamase Resistance: From Inhibitor Design To Substrate Evolution, Lian M.C. Jacobs

USF Tampa Graduate Theses and Dissertations

β-lactamase production by bacteria is a major mechanism of antibiotic resistance. These enzymes hydrolyze β-lactam antibiotics, preventing them from binding to and inhibiting PBPs. Although new β-lactam scaffolds and inhibitors are continually introduced into the clinic, resistance often emerges within a few years. Therefore, the development of cross-class inhibitors with novel scaffolds is critical to mitigate this resistance. In parallel, a deeper understanding of the structural and mechanistic basis of β-lactamase resistance is essential. Together, these approaches provide an effective strategy for combating antibiotic resistance.

The first project addressed the need of effective cross-class β-lactamase inhibitors capable of targeting SBL …

Unquaint Pigs’ Stakeholder Perspectives On Wild Swine In Central Western Florida, Tyler Koerner

USF Tampa Graduate Theses and Dissertations

The Anthropocene presents important questions for how to address the continuous transferal of new species into new spaces and the cascading impacts of environmental transformations. Novel ecosystems present one such outline for conservation that acknowledges the modification of historic ecosystems and the social entanglements that arrive with new species. I argue that novel ecosystems can benefit from further anthropological integrations that aid in describing the entanglements of introduced species and the pursuit of best managerial outcomes. For wild swine in Florida, the adoption of a novel approach is especially relevant because of entanglements with historic and modern Floridian stakeholders and …

The Heterogeneous Response Of Astrocytes In Different Brain Regions Under Metabolic Stress, Pawan Kumar Thapaliya

USF Tampa Graduate Theses and Dissertations

Astrocytes play a central role in maintaining ionic homeostasis, neurotransmitter clearance, and brain en-ergy metabolism. Their excitability, reflected in activity-evoked intracellular sodium (Na+, [Na+]i) and calcium (Ca2+,[Ca+]i) dynamics, is increasingly recognized as a critical determinant of neuronal function and vulnerability, particularly under metabolic stress. Despite their importance, the mechanisms underlying astrocytic ionic signaling and its regional heterogeneity remain incompletely understood. In this thesis, I developed detailed computational and biophysical models to investigate astrocytic Na+ and Ca2+ dynamics, their bioenergetic consequences, and the roles of key ion transporters under physiological and metabolically stressed conditions, validating the models against experi- mental data. …

An Equity Analysis Of Battery Electric Vehicles During Evacuation Considering Social And Financial Disadvantage, Divyamitra Mishra

USF Tampa Graduate Theses and Dissertations

The increasing share of battery electric vehicles (BEVs) in the transportation system is introducing new challenges to the emergency evacuation planning and management. During Hurricane Milton (2024), incidents involving BEVs, including battery fires, attributed to saltwater exposure risk, and advisories recommending relocation to higher ground, highlighted operational vulnerabilities unique to this technology. These emerging risks are likely to be experienced heterogeneously, as access to charging infrastructure, available resources, and evacuation flexibility varies across the population. This raises critical questions about how BEV-specific constraints interact with existing inequalities during evacuation. Motivated by this context, the dissertation investigated inequity in BEV evacuation …

Considerations For Cherenkov Radiation Imaging In External Beam Radiotherapy For Diverse Populations In A High Throughput Cancer Clinic, Diego A. Hernandez

USF Tampa Graduate Theses and Dissertations

Cherenkov radiation imaging is a burgeoning field of optical imaging that has become a promising, non-invasive, technique that allows the visualization of radiation delivery during external beam radiotherapy. This form of imaging has opened the door to real-time and retrospective treatment verification and sparked a plethora of research aimed at improving the utility. This dissertation investigates possible clinical implementations and limitations of Cherenkov radiation imaging within a high-throughput cancer clinic serving a diverse patient population. The central motivation is the need to advance Cherenkov radiation imaging as a reliable tool for clinical use by improving its quantitative accuracy and applicability …

Theory And Applications Of Natural And Nature-Based Solutions In Estuarine Environments, Elizabeth L. Royer

USF Tampa Graduate Theses and Dissertations

This dissertation focuses on the theory and application of Nature-based Solutions (NbS), through three unique case studies. This study explores how the broad and interdisciplinary NbS framework can be applied to mitigate common coastal hazards. This framework considers the environment, economics, and social wellbeing, equally in the design and implementation of a solution. The current paradigm, working against nature, which is commonly seen in more passive approaches to hazard mitigation, like grey infrastructure (e.g. a seawall to protect a shoreline against wave action) does not accommodate the extremely dynamic environment. As perspectives shift to “working with nature” NbS can be …

Iron Deficiency And Depressive Symptoms: Effects Of Racial And Ethnic Disparity In Two Reproductive-Aged Us Women, Alexis Monkhouse

USF Tampa Graduate Theses and Dissertations

Problem: Reproductive- aged women are one of the populations most at risk for iron deficiency and depression in the United States. While many studies have looked at the relationship between these two illnesses, none have explored it from a biocultural framework, examining how race/ethnicity affects this relationship. Using data from NHANES, this thesis examined the relationship between iron status (total body iron) and depressive symptoms (PHQ-9) among reproductive-aged women in the U.S. and how race/ethnicity affects it. Methods. NHANES survey data from 2005-2010 were pulled. Demographic and laboratory data were used to determine eligibility, total body iron, and depressive scores. …

Novel Electrical Interfaces For Bionic Limbs, Matthew Aaron Melton

USF Tampa Graduate Theses and Dissertations

Advancements in neural interfaces are pivotal for enhancing the functionality and longevity of implantable biomedical applications, particularly in the field of neuroprosthetics. This dissertation is focused on the continuing development of a novel neural interface fabricated from silicon carbide (SiC). SiC has demonstrated superior performance over traditional silicon-based material systems due to its exceptional biocompatibility, hemocompatibility, and electrochemical stability. The research herein explores silicon carbide’s semiconductor properties, with a highlighted focus on a unique and innovative design structure - centered around a p-n junction. This work examines detailed fabrication steps of an all-SiC device, acute-style packaging (for future in vivo …

Clinical Features, Laboratory Characteristics, And Outcomes Of Covid-19 Patients In A Tertiary Care Reference Hospital In Panama City, Panama. A Retrospective Observational Study, Santiago E. Hernandez Bojorge

USF Tampa Graduate Theses and Dissertations

Latin America experienced some of the world’s highest COVID-19 mortality rates, yet data on in-hospital outcomes and their social and geographic determinants remain limited. This dissertation investigated multilevel baseline factors associated with in-hospital mortality among adults with COVID-19 at Panama’s largest public tertiary hospital (Hospital Santo Tomás), integrating survival, mediation, and spatial-temporal modeling within a causal-inference framework.

A retrospective cohort of 1,526 RT-PCR–confirmed COVID-19 hospitalizations (May 2020–November 2021) was analyzed using multivariable Cox proportional-hazards, Fine–Gray competing-risk, mediation, and Bayesian spatial models. Directed acyclic graphs (DAGs) pre-specified causal relationships and minimally sufficient adjustment sets. DAG-guided models quantified total effects without conditioning …

From Marginalization To Prime Consideration: Nuclear Energy In Fragmented Climate Governance, Polarization, And The Age Of Ai, Abdullah Al Jaberi

USF Tampa Graduate Theses and Dissertations

In recent years, nuclear energy has remained sidelined in global climate discussions despite being the world’s second-largest source of low-carbon electricity. This dissertation examined why nuclear energy should be the global priority decarbonization strategy, based on an evaluation of its unique position across the following three dimensions: fragmented international climate governance, political polarization in democratic countries, and unprecedented baseload electricity demands created by artificial intelligence infrastructure. This study does not argue causal linkages among these dimensions; rather, it demonstrates that nuclear energy succeeds concurrently across all three. A mixed-methods approach was employed, including document analysis of bilateral agreements and official …

Temporally Modulated Acoustic Environment Alters Inferior Colliculus Tonotopy And Improves Neural Gap Encoding In Aged Cba/Caj Mice, Luis Franco-Waite

USF Tampa Graduate Theses and Dissertations

Age-related hearing loss (ARHL) is an omnipresent and progressive decline of hearing that occurs later in life and affects a significant majority of the aged population. While the current accommodations for hearing loss focus on improving comfort and quality of life, they do not address the underlying neural aspects of ARHL. A novel approach for treatment is the augmented acoustic environment (AAE) which utilizes the innate plasticity of the nervous system in response to the external environment. The work presented here documents the research performed on passive noise exposures which target neural aspects in animal models of age-related hearing loss. …

Death And Things: Toward A Theory Of Self-Holding Objects, Kirsten J. Lee

USF Tampa Graduate Theses and Dissertations

This dissertation examines how material objects function as existential supports under conditions of cultural exclusion. Drawing on Terror Management Theory (TMT), minority stress theory, and José Muñoz’s concept of disidentification, it introduces the construct of self-holding objects: possessions imbued with identity-sustaining meaning that stabilize the self. Study 1 employed a qualitative photovoice design with 16 queer adults and identified five primary existential functions of meaningful objects: affirming worth, regulating the body, mirroring the self, authorizing possibility, and enabling worldmaking. These findings suggest that objects operate as material anchors within distributed systems of meaning. Study 2 experimentally tested whether imagining the …

Mentoring Mentalities Made Manifest: Toward A Better Understanding Of Secondary Teacher Mentor Professional Learning, Practice, And Self-Efficacy, Maggie L. Wilson

USF Tampa Graduate Theses and Dissertations

Teacher shortages are becoming increasingly problematic nationwide. Teacher mentoring has been found to be an effective method of curbing teacher attrition, but little attention has been addressed to ensuring that teacher mentors are receiving the most relevant professional learning experiences to bolster their self-efficacy and mentoring practice. This qualitative study, drawing on research traditions such as grounded theory and case study, seeks to illuminate the mentor self-efficacy and practice of secondary teacher mentors and to understand the role their professional learning plays in how and how well they enact their mentor roles. Through qualitative interviews, including artifact-based interviews, this study …

The Molecular Determinants Of Deglycation And Substrate Specificity In Fructosamine Kinases, Jenet Matlack

USF Tampa Graduate Theses and Dissertations

Protein glycation is a universal, non-enzymatic modification that occurs when a sugar covalently attaches to a primary amine. These spontaneous modifications may have deleterious or regulatory effects on protein function, and their removal is mediated by the conserved metabolic kinase fructosamine-3-kinase (FN3K). Despite its crucial role in protein repair, we currently have a poor understanding of how FN3K engages or phosphorylates its substrates. Additionally, the molecular basis for the distinct yet overlapping substrate specificities of FN3K and its paralog, ketosamine-3 kinase (KT3K), remains unresolved. By integrating structural biology, biochemistry, and ancestral protein reconstruction (APR), this thesis elucidates the catalytic mechanism …

Teachers' Perspectives On The Inclusion Of Children With Autism In Preschool And Kindergarten Programs Within Inclusive Classrooms In Saudi Arabia, Budur Alsalamah

USF Tampa Graduate Theses and Dissertations

In recent years, Saudi Arabia has witnessed a significant increase in the number of students with autism being integrated into mainstream classrooms. This is thanks to laws and regulations enacted to provide appropriate education for students with special needs, particularly those with autism, in less restrictive environments. This is a positive step towards providing equal educational opportunities for students with special needs. However, teachers' views on integrating children with autism into mainstream education remain varied. Studies have shown that teachers with positive attitudes towards inclusion are more likely to provide inclusive education in the classroom (Dev & Hayes, 2015). This …

Invasion Risk Of Regal Demoiselle Following Lionfish Invasion In The Western Atlantic Ocean, Gulf Of Mexico, Bahamas, And Greater Caribbean, Shea L. Volkel

USF Tampa Graduate Theses and Dissertations

Indo-Pacific lionfish (Pterois volitans and P. miles) and regal demoiselle(Neopomacentrus cyanomos) are reef-associated fishes that have invaded the Western Atlantic Ocean, Gulf of Mexico, Bahamas, and Greater Caribbean. While invasive lionfish are established mesopredators with documented impacts on native reef communities, regal demoiselle are at an earlier stage of invasion, and their ecological risks are unclear. Because lionfish consume regal demoiselle in their invaded range, interactions between these species, particularly predation, may influence regal demoiselle invasion success. This dissertation assessed the invasion risk of regal demoiselle, focusing on predator-prey interactions among these species. The objectives were to 1) identify areas …

Making Sense Of The Body: Cognitive Science, Disability, And Embodiment, Cheyne L. Joslin

USF Tampa Graduate Theses and Dissertations

The purpose of this project is to analyze different methodological approaches in the philosophy of cognitive science, with the goal of determining their usefulness for explaining and understanding bodily awareness and skill-acquisition over time. The examples of skill-acquisition are limited to motor control, impaired by various kinds of disability—but then acquired over time, via technological or rehabilitative means. These phenomena of motor learning are then viewed through the lens of two competing theoretical approaches in cognitive science: neuromechanical cognitive science, and radical enactivist cognitive science.

The strengths of the neuromechanical framework are found in its historical pedigree, and in the …

Transport And Aging Pathways Of Microplastics In Urban Aquatic Systems, Jessica J. Boyer

USF Tampa Graduate Theses and Dissertations

Microplastics are pervasive in urban aquatic systems, which serve as both conduits of particle flow and incubators of microplastics. These aquatic systems transport microplastic particles while simultaneously altering their physical, chemical, and biological properties. Despite their growing environmental relevance, constructed wetlands and riverine systems remain underrepresented in studies of microplastic fate and transport. This dissertation aims to assess the patterns influencing microplastic transport and aging across urban aquatic environments through a combination of field experiments and mechanistic modeling.

Four interconnected studies were conducted to address this objective. The first investigated the horizontal and vertical transport of microplastics within the Se7en …

Representations Of Motherhood In 19th And 20th Century American Literature, Zabrina L. Shkurti

USF Tampa Graduate Theses and Dissertations

This dissertation examines how American women writers of the 19th and early 20th centuries constructed, contested, and reimagined motherhood as both a lived experience and a powerful social institution. Drawing on feminist theory and motherhood studies—particularly Adrienne Rich’s distinction between motherhood as experience and motherhood as institution and Shari Thurer’s definition of the “good mother”—it argues that this institution and ideology were never stable or universally attainable. Instead, it functioned as a shifting, exclusionary construct shaped by race, class, industrial capitalism, and patriarchal authority. Through close readings of domestic literature, poetry, fiction, and reformist texts by writers including Elizabeth Oakes …

Is There No Such Thing As Bad Pr? A Case Study Analysis Of Influencer Pseudo-Events, Authentic Crises, And Crises As Opportunity, And Paracrisis, Sofia Aparicio-Gallagher

USF Tampa Graduate Theses and Dissertations

There's no such thing as bad PR, and any publicity is good publicity are phrases coined by 19th-century P.T. Barnum, a showman and businessman known for his circus hoaxes (Hiergesell, 2025). The idea implies that all media attention, whether negative or positive, is beneficial to the organization or individual. The purpose of this study is to evaluate three brands; Tarte, Poppi, and Shein during a brand event to determine whether it was a pseudo-event created to gain awareness, or a genuine, unplanned crisis. Another purpose of the study was to determine if the event was used as an opportunity for …

Examining The Effect Of Household Socioeconomic Status And Maternal Characteristics On Child Developmental Delay In The United States, Samuel Ilori

USF Tampa Graduate Theses and Dissertations

Child developmental delay remains a pressing public health issue with lifelong implications. This study examines the association between household socioeconomic status, maternal characteristics, and child developmental delay using data from the 2023 National Survey of Children's Health (N = 35,399 female respondents). Bivariate analyses using Chi-square tests and binary logistic regression with sequential modeling were conducted. Results revealed that economic hardship, employment status, maternal mental health, maternal physical health, marital status, and race were significantly associated with child developmental delay (p<0.001). Educational level and poverty level showed no significant associations. Among mothers reporting poor mental health, 90.0% had a child with a delay, compared to 6.6% among those with good mental health. Every mother reporting poor physical health had a child with a delay, while virtually none with good or better physical health did. In the fully adjusted regression model, maternal mental and physical health remained the most powerful predictors, while economic hardship and employment status became non-significant once health was introduced, suggesting mediation. Maternal mental and physical health are the factors most strongly correlated with child developmental delay. Economic hardship matters primarily because it undermines maternal well-being. The COVID-19 pandemic likely intensified these dynamics by disrupting healthcare access and increasing economic instability. Supporting maternal health must be recognized as a fundamental strategy for promoting child development. Universal screening for maternal depression and physical health problems, integrated into pediatric and obstetric care, can identify at-risk mothers early. Policies that reduce economic hardship alleviate the chronic stress that undermines maternal health. Children thrive when their mothers are healthy.

The Development And Validation Of A Portable Monitoring System For Real-World Prosthetic And Orthotic Performance Assessment, Emily Wyatt

USF Tampa Graduate Theses and Dissertations

Laboratory-based motion capture systems are widely used for gait analysis but are limited by high cost, infrastructure requirements, and restricted external validity. Inertial measurement units (IMUs) offer a portable and low-cost alternative for assessing gait in both controlled and real-world environments; however, their accuracy and clinical utility require validation. The purpose of this thesis was to develop and evaluate a portable monitoring system using IMUs to quantify spatiotemporal gait parameters and gait asymmetry in both laboratory and community settings, with a focus on prosthetic and orthotic users.

Phase 1 of this study evaluated the agreement between IMU-derived gait metrics and …