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Early Developmental Impacts On Male Traits And Female Preference In Zebra Finches (Taeniopygia Guttata), Martyna Boruta

USF Tampa Graduate Theses and Dissertations

Some male sexually selected traits are sensitive to stressors early in life and provide females with information to discriminate among males with different developmental experiences. Moreover, female early life experiences could also impact which males they choose. Females might either choose honest traits indicative of male quality, no matter their own experiences, or they might choose mates to match or compensate for their own experiences. To determine how developmental stressors alter male sexually-selected traits and female preference thereof, I exposed zebra finches (Taeniopygia guttata, ZEFI) to i) lipopolysaccharide (LPS), an immunogenic, Gram-negative bacterial component, ii) corticosterone (CORT), an avian stress …

Synthesis, Characterization And Mechanistic Studies Of Biomolecules@Mesomofs, Yao Chen

USF Tampa Graduate Theses and Dissertations

Encapsulation of biomolecules is of great interest to research advances related to biology, physiology, immunology, and biochemistry, as well as industrial and biomedical applications such as drug delivery, biocatalysis, biofuel, food and cosmetics. Encapsulation provides functional characteristics that are not fulfilled by free biomolecules and stabilizes the fragile biomolecules. In terms of biocatalysis, solid support can often enhance the stability of enzymes, as well as facilitate separation and recovery for reuse while maintaining activity and selectivity. Various kinds of materials have been used for encapsulation of biomolecules, among which, porous materials are an important group. Metal-organic frameworks (MOFs) have attracted …

Topological Data Analysis Of Properties Of Four-Regular Rigid Vertex Graphs, Grant Mcneil Conine

USF Tampa Graduate Theses and Dissertations

Homologous DNA recombination and rearrangement has been modeled with a class of four-regular rigid vertex graphs called assembly graphs which can also be represented by double occurrence words. Various invariants have been suggested for these graphs, some based on the structure of the graphs, and some biologically motivated.

In this thesis we use a novel method of data analysis based on a technique known as partial-clustering analysis and an algorithm known as Mapper to examine the relationships between these invariants. We introduce some of the basic machinery of topological data analysis, including the construction of simplicial complexes on a data …

Can The Usa National Weather Service Heat Index Substitute For Wet Bulb Globe Temperature For Heat Stress Exposure Assessment?, Ivory Iheanacho

USF Tampa Graduate Theses and Dissertations

Heat stress occurs when the body cannot adequately cool itself due to the combined contributions of metabolic heat, environmental factors and clothing. Heat stress found in the workplace puts employees at risk of developing heat-related illnesses, disorders and could be fatal. The wet bulb globe temperature (WBGT) index is the current method used to assess environmental contributions to heat stress in an occupational setting. The purpose of this thesis was to explore whether the National Weather Service's Heat Index (HI) could substitute for the WBGT Index during occupational heat stress exposure assessment. The possibility of using the HI for heat …

From Co2 To Cell: Energetic Expense Of Creating Biomass Using The Calvin-Benson-Bassham And Reductive Citric Acid Cycles Based On Genomic Data, Mary Ann Mangiapia

USF Tampa Graduate Theses and Dissertations

Abstract

The ubiquity of the Calvin-Benson-Bassham cycle (CBB) amongst autotrophic organisms suggests that it provides an advantage over a wide range of environmental conditions. However, in some habitats, such as hydrothermal vents, the reductive citric acid cycle (rCAC) is an equally predominant carbon fixation pathway. It has been suggested that the CBB cycle poses a disadvantage under certain circumstances due to being more energetically demanding compared to other carbon fixation pathways. The purpose of this study was to compare the relative metabolic cost of cell biosynthesis by an autotrophic cell using either the CBB cycle or the rCAC. For both …

The Relationship Of Affective Domains And Cognitive Performance In Paramedic Students, Katie J. Lyman

USF Tampa Graduate Theses and Dissertations

No universal approach for application procedures has been established for paramedic curriculum programs. The field of pre-hospital, Emergency Medical Services (EMS) has evolved quickly from its inception to present date. The educational components of EMS are still in their infancy and lack evidence-based protocols (Drees, 2006). Predicting success in an allied health program typically concentrates on some type of academic instrument. The use of personality inventories has been underexplored; however, literature reveals they may be more reliable in determining academic and employment success compared with other non-cognitive tools (Groves, Gordon, & Ryan, 2007; Marrin et al., 2004; McManus & Richards, …

Characterization Of Waste Anesthetic Gas Exposures To Veterinary Workers In The Tampa Bay Area, Kyle Vogel

USF Tampa Graduate Theses and Dissertations

Veterinarians routinely conduct surgical operations on animals while veterinary technicians administer anesthetic gas to sedate the animal prior to an operation. One commonly used anesthetic agent in veterinary clinics is isoflurane. Veterinary workers have the potential to be exposed to this gas during surgical operations. The Occupational Safety and Health Administration (OSHA) does not have a specific Permissible Exposure Limit (PEL) for isoflurane, however The National Institutes for Health (NIH) does advise that workers should not be exposed to isoflurane in concentrations exceeding 2 parts per million (ppm) for an 8 hour Time Weighted Average (TWA).

Animal clinics vary in …

Negotiating Muslim Womanhood: The Adaptation Strategies Of International Students At Two American Public Colleges, Amber Michelle Gregory

USF Tampa Graduate Theses and Dissertations

From a Western perspective, North Americans and Western Europeans perceive Muslim women as being oppressed (Andrea 2009; Lutz 1997, 96; Ozyurt 2013). Led by this assumption, some view studying abroad as an international student as an experience that allows Muslim women the opportunity to "escape" this supposed oppression and to know "freedom" in the U.S. However, Muslim women's experiences are more dynamic and complex than this dualism suggests. In this thesis, I explore adaptation strategies of Muslim women international students, and how gender, race, and religion affect their experiences while abroad. Furthermore, I explore the women's use of emotion management …

Investigating The Diversity Of Single-Stranded Dna Bacteriophages In Marine Environments, Max Stephen Hopkins

USF Tampa Graduate Theses and Dissertations

There are estimated to be 1030 virus-like particles in the world's oceans. Most are viruses that infect bacteria, called `bacteriophages' or simply `phages'. Phages exert tremendous influence on marine biogeochemical cycling because they are responsible for about half of all bacterial death in the oceans, causing nutrient release into the dissolved and particulate organic matter pools. Traditional paradigms of phage biology held that most of these ocean phages belonged to the Caudovirales group: phages that contain a double-stranded DNA genome within a geometric capsid `head' to which a `tail' is joined, in one of several morphological variants, that is the …

Insights Into The Epitaxial Relationships Between One-Dimensional Nanomaterials And Metal Catalyst Surfaces Using Density Functional Theory Calculations, Debosruti Dutta

USF Tampa Graduate Theses and Dissertations

This dissertation involves the study of epitaxial behavior of one-dimensional nanomaterials like single-walled carbon nanotubes and Indium Arsenide nanowires grown on metallic catalyst surfaces. It has been previously observed in our novel microplasma based CVD growth of SWCNTs on Ni-Fe bimetallic nanoparticles that changes in the metal catalyst composition was accompanied by variations in the average metal-metal bond lengths of the nanoparticle and that in turn, affected nanotube chirality distributions. In this dissertation, we have developed a very simplistic model of the metal catalyst in order to explain the nanotube growth of specific nanotube chiralities on various Ni-Fe catalyst surfaces. …

Development And Assessment Of Self-Explaining Skills In College Chemistry Instruction, Adrian Villalta-Cerdas

USF Tampa Graduate Theses and Dissertations

The prevalent trend in chemistry instruction relies on what has been described as the classroom game. In this model, students take a passive role and the instructor does all the explaining (thinking), and learning is trivialized to knowing the correct answers (memorizing) and being able to produce them when prompted (regurgitating). The generation of explanations is central to scientific and technological development. In the process of figuring out explanations, the generation of inferences relies on the application of skills associated with scientific behaviors (e.g., analytical reasoning and critical thinking). The process of explanation generation causes a deeper analysis and revision …

Novel Cell Cycle Proteins In Apicomplexan Parasites, Carrie Butler

USF Tampa Graduate Theses and Dissertations

Apicomplexans are responsible for major human diseases such as toxoplasmosis caused by Toxoplasma gondii (T. gondii) and the deadliest form of malaria caused by Plasmodium falciparum (P. falciparum). The genomes of these pathogens are now sequenced ushering in a new era of drug development. A major hurdle to exploiting this genome resource is that a large number of the encoded genes are "hypotheticals" and have yet to be characterized. Hypothetical proteins comprise roughly half of the predicted gene complement of T. gondii and P. falciparum and represent the largest class of uniquely functioning proteins in these parasites.

Following the idea …

Age-Associated Increases In Fkbp51 Facilitate Tau Neurotoxicity, Laura J. Blair

USF Tampa Graduate Theses and Dissertations

Tau is a protein which regulates microtubule stability and is heavily involved in axonal transport. This stability is dynamically controlled in part by over 40 phosphorylation sites across the tau protein which allows for binding and release from the microtubules. However, if abnormal hyperphosphorylation occurs, tau dissociates from the microtubules. Once released, the microtubules become unstable and the aberrant tau mislocalizes from the axon to the somatodendric compartment, where it aggregates. These aggregates are made of many pathological forms of tau including oligomeric species, paired helical filaments, and neurofibrillary tangles, all of which have associated toxicities. Tau pathology is a …

Diffuse Nutrient Pollution From Residential Catchments, Melissa Rachelle Butcher

USF Tampa Graduate Theses and Dissertations

Nonpoint source nutrient pollution is diffuse pollution lacking discrete origin and conveyance. This thesis synthesizes and critically reviews research on residential nitrogen and phosphorus loss to stormwater runoff and leaching. The evaluation pulls from research covering influential socio-demographic indicators, such as use of lawn maintenance services and homeowner fertilizer practices. The extent to which such social and economic factors may influence the prevalence and fate of diffuse nutrients in stormwater runoff from residential areas has not been adequately established. Understanding the source and influencing factors of diffuse nutrient pollution is important in order to effectively protect surface and groundwater resources. …

Inactivation Of Ascaris In Double-Vault Urine-Diverting Composting Latrines In Panama: Methods And Environmental Health Engineering Field Applications, Daragh A. Gibson

USF Tampa Graduate Theses and Dissertations

The United Nations Millennium Development Goals have prioritized improving access to sanitation, but unfortunately about a third of the global population is still without an improved sanitation source and one billion still practice open defecation. Lack of access to adequate and safe sanitation means the proliferation of dangerous pathogens in the environment, especially soil-transmitted helminths (STHs). In the Bocas del Toro Province of Panama (and similar locations in the world), composting latrines have been built in many of the indigenous communities in the area. They are a form of dry or ecological sanitation and are designed to produce an end …

"Is That What You Dream About? Being A Monster?": Bella Swan And The Construction Of The Monstrous-Feminine In The Twilight Saga, Amanda Jayne Firestone

USF Tampa Graduate Theses and Dissertations

ABSTRACT

This dissertation argues that Bella Swan is a representation of Barbara Creed's monstrous-feminine which serves to reinforce ideologies that insist women are abject, inherently dangerous to men, and threatening to a patriarchal status quo. Through close-textual analysis of The Twilight Saga, I demonstrate how the monstrous-feminine frames the hysterical teenage body, hypersexuality, and eternal motherhood as simultaneously unacceptable and unavoidable. These negative women's stereotypes continue to persist in dominant popular culture, and this doublebind is overcome only by the impossible perfection of vampirism. The monstrous-feminine invites constructions of teenage bodies as unstable and unreliable, women's sexuality as dangerous and …

Water And Health In The Nandamojo Watershed Of Costa Rica: Community Perceptions Towards Water, Sanitation, And The Environment, James Mcknight

USF Tampa Graduate Theses and Dissertations

Understanding the relationships between human health, water, sanitation, and environmental health is a requirement to understanding the challenges that face researchers when it comes to addressing global health relating to water and sanitation. Access to improved water and sanitation is not only a precondition to health, but to all aspects of daily living. Target 7.C of the Millennium Development Goals (MDGs) addresses worldwide disparities in access to improved water and sanitation by calling for the reduction in "half of the proportion of people without sustainable access to safe drinking water and sanitation by 2015". Over 90% of the population of …

Targeting Cancer Metabolism With Ketosis And Hyperbaric Oxygen, Angela M. Poff

USF Tampa Graduate Theses and Dissertations

Cancer cells exhibit an abnormal metabolic phenotype characterized by glycolysis and lactate fermentation in the presence of oxygen, a phenomenon known as the Warburg effect. This dysregulated metabolism plays an important role in every aspect of cancer progression, from tumorigenesis to invasion and metastasis. The Warburg effect is a common phenotype shared by most, if not all, cancer types. It is especially prominent in metastatic tumors, which are notoriously resistant to treatment and responsible for the majority of cancer-related deaths. Thus, metabolic therapies which target the Warburg effect could offer novel therapeutic options for most cancer patients, including those with …

Using Otolith Elemental Composition To Track The Habitat Use, Movements, And Life History Patterns Of Common Snook (Centropomus Undecimalis) And Red Drum (Sciaenops Ocellatus) In The Tampa Bay Estuary, Holly Jacqueline Rolls

USF Tampa Graduate Theses and Dissertations

Knowledge of fish habitat use and connectivity is critical for understanding the structure and dynamics of fish populations and, therefore, necessary for the implementation of successful fisheries management strategies. Tagging is an effective means of providing such information, and the elemental composition contained within fish otoliths is increasingly being used as a natural tag. The chemical composition of otoliths reflects the incorporation of elements from different water bodies and can thus be used to understand the habitat use, movements, and life history patterns of fishes. To assess the applicability of otolith elemental composition as a tagging technique within the Tampa …

Rtemis: Real-Time Tumoroid And Environment Monitoring Using Impedance Spectroscopy And Ph Sensing, Frank Alexander

USF Tampa Graduate Theses and Dissertations

This research utilizes Electrical Impedance Spectroscopy, a technique classically used for electrochemical analysis and material characterization, as the basis for a non-destructive, label-free assay platform for three dimensional (3D) cellular spheroids. In this work, a linear array of microelectrodes is optimized to rapidly respond to changes located within a 3D multicellular model. In addition, this technique is coupled with an on chip micro-pH sensor for monitoring the environment around the cells. Finally, the responses of both impedance and pH are correlated with physical changes within the cellular model. The impedance analysis system realized through this work provides a foundation for …

Solution Processable Novel Organic Electronic Devices For New Generation Biomedical Applications, Munish Puri

USF Tampa Graduate Theses and Dissertations

The following dissertation addresses a novel low cost process developed to fabricate a Vertical Organic Field Effect Transistor (VOFET). The solution processable VOFET is designed, fabricated and tested in the context of bioengineering domains. The scope of distinct biomedical applications has also been explored.

Organic thin-film transistors are gathering industrial attention as a potential candidate for future electronics analogous to silicon technology. Low fabrication cost, structural miniaturization and low operational voltage are the challenges for fabricating an Organic Field Effect Transistor (OFET). To create these devices, OFETs require new design paradigms and wet processing routes. However, conventional lateral OFET geometry …

Trend Analysis And Modeling Of Health And Environmental Data: Joinpoint And Functional Approach, Ram C. Kafle

USF Tampa Graduate Theses and Dissertations

The present study is divided into two parts: the first is on developing the statistical analysis and modeling of mortality (or incidence) trends using Bayesian joinpoint regression and the second is on fitting differential equations from time series data to derive the rate of change of carbon dioxide in the atmosphere.

Joinpoint regression model identifies significant changes in the trends of the incidence, mortality, and survival of a specific disease in a given population. Bayesian approach of joinpoint regression is widely used in modeling statistical data to identify the points in the trend where the significant changes occur. The purpose …

The Reproductive Lives Of Chuukese Women: Transnationalism In Guam And Chuuk, Sarah Ann Smith

USF Tampa Graduate Theses and Dissertations

Chuuk, one state of the Federated States of Micronesia (FSM), experiences significant transnational migration to the United States (U.S.), particularly to the Territory of Guam. This migration is facilitated by the Compact of Free Association (COFA), an agreement with several Micronesian countries previously under U.S. administration that allows for free movement of their citizens into the U.S. Although part of Micronesia, Guam's colonized residents resist an identity connected to rest of Micronesia. With very poor health outcomes, the Chuukese represent a political and social body of bodies that bring sickness, babies and increased costs to the Guam government without adequate …

Illness Perceptions Of Polycystic Ovary Syndrome, Elizabeth Baker

USF Tampa Graduate Theses and Dissertations

Polycystic ovary syndrome (PCOS) is a chronic illness that affects approximately five million premenopausal women in the United States and is associated with significant cosmetic, reproductive, metabolic, and psychological consequences. Despite its prevalence, few studies have explored the lived experiences and illness perceptions of women living with PCOS. Identifying illness perceptions of women living with (WLW) PCOS is important, because mounting research suggests that a person's perceptions of their chronic illness and its management determine that person's coping behaviors (e.g., adherence, self-management) and, consequently, illness outcomes.

In this dissertation, the Common Sense Model (CSM) is used as a framework to …

The "Defective" Generation: Disability In Modernist Literature, Deborah Susan Mcleod

USF Tampa Graduate Theses and Dissertations

Abstract

The "Defective" Generation: Disability in Modernist Literature aims to provide an analysis of how Anglo-American authors in the early twentieth century conceived of, utilized, and portrayed disability in their fiction. Building on the existing scholarship in the field of Disability Studies, I argue that modernists revise the tradition of representation to make disabilities a generational trait rather than a sign of individual deviance. In novel after novel, multiple characters exhibit some form of illness or impairment, which appears as both cause and effect of the instabilities and traumas of modernity. Like many of their predecessors, then, these authors portray …

Measuring The Adaptation Of Military Response During The Second Seminole War Florida (1835-1842): Kocoa And The Role Of A West Point Military Academy Education, Michelle Diane Sivilich

USF Tampa Graduate Theses and Dissertations

Conflict archaeology is a fairly new discipline and is in the process of defining its methods and theories. Recently, the American Battlefield Protection Program has started requiring that grant applicants perform a KOCOA analysis. KOCOA is a modern military technique and stands for Key terrain, Obstacle, Cover and Concealment, Observation, and Avenues of Approach. However, this method was developed for modern warfare, and its adoption by the archaeological community has not yet been analyzed. I argue that this method needs a few modifications to make it more applicable to historical research and that it can be broadened to investigate more …

Perceived Discrepancies In Men's Motivations For Gender-Conforming Behaviors And Romantic Relationship Outcomes, Jonathan Robert Weaver

USF Tampa Graduate Theses and Dissertations

Healthy romantic relationships are positively associated with physical and mental health outcomes, and past research has shown that traditional masculinity negatively impacts relationship satisfaction. The current study examined the effects of men's discrepancies between their self-ratings and perceptions of their peers' on autonomous and pressured motivations to act agentically on relationship outcomes for both partners in a heterosexual relationship. In addition, men's investment in gender ideals was measured as a potential moderator and men's self-esteem as a potential mediator. Specifically, it was predicted that men's self-esteem would mediate the association between the investment-by-discrepancies (autonomous and pressured) interactions and relationship satisfaction …

Statistical Analysis, Modeling, And Algorithms For Pharmaceutical And Cancer Systems, Bong-Jin Choi

USF Tampa Graduate Theses and Dissertations

The aim of the present study is to develop a statistical algorithm and model associ- ated with breast and lung cancer patients. In this study, we developed several statistical softwares, R packages, and models using our new statistical approach.

In the present study, we used the five parameters logistic model for determining the optimal doses of a pharmaceutical drugs, including dynamic initial points, an automatic process for outlier detection and an algorithm that develops a graphic user interface(GUI) program. The developed statistical procedure assists medical scientists by reducing their time in determining the optimal dose of new drugs, and can …

Self-Control Depletion And Nicotine Deprivation As Precipitants Of Smoking Cessation Failure: A Human Laboratory Model, Bryan Heckman

USF Tampa Graduate Theses and Dissertations

The need to understand the reinforcing properties of smoking and potential precipitants of relapse is exemplified by evidence that relapse rates exceed 95%. The Self-Control Strength model, which proposes that self-control is dependent upon limited resources and susceptible to fatigue, may offer insight into the relapse process. Indeed, there is empirical support that engaging in a task that requires self-control, relative to a comparable control, results in performance decrements on subsequent self-control tasks. The primary goal of the current study was to test whether self-control depletion (SCD) may serve as a novel antecedent for cessation failure, using a validated laboratory …

Associations Between Student-Teacher Relationships And Kindergarten Students' Outcomes, Leslie Marie Wells

USF Tampa Graduate Theses and Dissertations

The student-teacher relationship (STR) is an important component of a student's success in school. STRs have the potential to serve as an asset for students' well-being and achievement throughout their school career. Current literature suggests that there are two major components of STRs associated with student's academic and behavioral outcomes: closeness and conflict. Research has indicated that STRs characterized by closeness are linked to positive academic and behavior outcomes for students while STRs characterized by conflict are associated with negative academic and behavior outcomes for students. Although research has demonstrated that closeness and conflict have an impact on student outcomes, …