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A Multi-Scale Approach To Study Predator-Prey Interactions And Habitat Use Of Pinfish, Lagodon Rhomboids, Dinorah Helena Chacin

USF Tampa Graduate Theses and Dissertations

Biological processes like species interactions and patterns such as abundance and distribution observed in nature can vary depending on the scale at which the subject of interest is evaluated. Knowing that there is no single natural scale at which systems should be studied, in this thesis, I conducted a series of basic and applied ecological approaches in order to examine the phenomena that can occur at different scales of space, time, and ecological organization.

Species abundances can vary over large spatial and temporal scales. By studying the habitat use of an abundant species, which uses a wide range of habitats, …

Interpolating Beach Profile Data Using Linear And Non-Linear Functions, Lance Calloway Croft

USF Tampa Graduate Theses and Dissertations

ABSTRACT

Beach and nearshore surveys are conducted in a variety of ways, the most commonly used being the level-and-transit method; because it is inexpensive, time conducive and highly accurate. Specifically, beach surveys are conducted to better understand cross-shore, long-shore sediment transport processes, as well as to quantify volume changes, which are used to evaluate beach performance. In this study, a section of the beach on Sand Key, FL was surveyed using rod-and-transit. In addition to the commonly used linear data analysis, a non-linear analysis was conducted using NURBS (Non-Uniform Rational B-Splines).

Survey data was collected within a short time window …

Bi-Directional Signaling Between Melanoma And The Microenvironment Generates A Protective Niche That Mediates Therapeutic Escape, Inna Fedorenko

USF Tampa Graduate Theses and Dissertations

Very few cancer patients are cured through drug therapy alone, with the majority exhibiting acquired resistance. To date, most studies of therapeutic escape have focused upon tumor-intrinsic mechanisms of drug resistance with little attention paid to the role of normal host cells in preventing complete tumor eradication. In the present study we implicate co-operative bi-directional signaling between melanoma cells and fibroblasts in the generation of a pro-survival niche that mediates drug resistance. Mass-spectrometry based phosphoproteomics was used to show that BRAF inhibition and chemotherapy drugs enhanced the survival of both melanoma cells and fibroblasts through the induction of fibronectin (FN)/integrin …

Feeding Performance Of King Mackerel, Scomberomorus Cavalla, Amber Rose Ferguson

USF Tampa Graduate Theses and Dissertations

Although bite force is a commonly used metric of feeding performance, other factors such as bite pressure and strike speed are also likely to affect prey capture. Therefore, this study investigated static bite force, dynamic speeds, and predator and prey forces resulting from ram strikes, as well as bite pressure of the king mackerel, Scomberomorus cavalla, in order to examine their relative contributions to overall feeding performance. Theoretical posterior bite force ranged from 14.0-318.7 N. Ram speed, recorded with a rod and reel incorporated with a line counter and video camera, ranged from 3.3-15.8 BL/s. Impact forces on the …

Incredi-Bull-Ly Inclusive?: Assessing The Climate On A College Campus, Aubrey Lynne Hall

USF Tampa Graduate Theses and Dissertations

Lesbian, gay, bisexual, and transgender (LGBT) students have unique experiences on campus such as discrimination, exclusivity, and homo/transphobia. Stated simply, this research project intends to address these issues by 1) identify students' perceptions of gender identity and sexual orientation diversity on campus, 2) identify the experiences of LGBT students during their time at the institution, and 3) acknowledge suggestions from the student body for ways the University being studied is, or may continue to be, inclusive of sexual orientation and gender identity.

Through the application of the campus climate framework and modification of existing climate surveys, a student-centered campus climate …

Bartonella Henselae Infection And Host Response In The Zebrafish Embryo Model, Amorce Lima

USF Tampa Graduate Theses and Dissertations

The Gram-negative bacterium Bartonella henselae (Bh) is an emerging zoonotic pathogen that has been associated with a variety of human diseases including bacillary angiomatosis which is characterized by vasoproliferative tumor-like lesions on the skin and internal organs of some immunosuppressed individuals. Several virulence factors associated with Bartonella-induced pathogenesis have been characterized. However, the study of those virulence factors has been limited to in vitro cell culture systems due to the lack of a practical animal model. Therefore, we wanted to investigate whether the zebrafish embryo (Danio rerio) could be used to model human infection with Bh. We investigated …

31p Dynamic Nuclear Polarization Applied To Dimethyl Methyl Phphonate For Functional Imaging And Spectroscopic Studies, Roha Afzal

USF Tampa Graduate Theses and Dissertations

In the recent years, Dynamic Nuclear Polarization (DNP) has emerged as a very promising technique for enhancing the sensitivity of the magnetic resonance spectroscopy and imaging (MRSI). A number of nuclei, namely 13C, 15N, 29Si, 89Y, and 129Xe, have been successfully polarized and a few of them have been employed in the in-vivo studies for functional imaging and metabolism. Hyperpolarized 13C-labeled compounds have wide applications in the metabolic and perfusion studies and can be used for early stage disease diagnosis, response to treatment, prognosis etc. DNP has been demonstrated in the 31P nucleus in nucleotides triphosphates as an application for …

Localized Expansion Of Pedicle Screws For Increased Stability And Safety In The Osteoporotic Spine, Thomas Michael Shea

USF Tampa Graduate Theses and Dissertations

When a patient is diagnosed with various spinal injuries, deformities, or advanced degeneration, it is commonly suggested that he/she undergoes surgery for spinal fusion. Most current procedures in spinal fusion restrict mobility in one or multiple levels of the spine so that, over time, new bone will grow between the levels creating a single motionless unit of bone. The bilateral pedicle screw system (BPSS) has long been considered to be the "gold standard" in spinal fusion. However, for patients with osteoporosis, adequate fixation within the bone-screw interface has continuously been difficult to achieve or has come with high risk of …

Can Anyone With Low Income Be Food Secure?: Mitigating Food Insecurity Among Low Income Households With Children In The Tampa Bay Area, Edgar Allan Amador

USF Tampa Graduate Theses and Dissertations

In the US over the last few years, approximately 14.5% of households experience food insecurity at some point throughout the year. While studies on food insecurity in the US have determined that household income and specifically income available to spend on food is of critical importance to food security, it is still unclear why some households with low income are able to maintain food security while others experience food insecurity in a pattern characterized as not constant but recurrent. This dissertation compares households with children at different levels of food security and insecurity using the USDA Core Food Security Module …

Are Gopher Tortosies (Gopherus Polyphemus Daudin) Comapatible With Cows?, Thomas William Hentges

USF Tampa Graduate Theses and Dissertations

Many Gopher Tortoise populations are in steep decline throughout Florida, and various measures have been attempted to curb the trend. One such measure is to relocate tortoises to protected recipient sites on private lands. The majority of private lands in Florida are used for cattle, however, production and the effect of cattle production on tortoises is not known. Here, I tested six parameters of tortoise behavior by monitoring 1403 gopher tortoises released at the Barthle Brothers Ranch, Pasco County Florida, between August 2009 and December 2012. The parameters tested were (1) burrow density, (2) burrow spacing, (3) burrow relocation, (4) …

Evaluation And Modeling Of Internal Water Storage Zone Performance In Denitrifying Bioretention Systems, Thomas Joseph Lynn

USF Tampa Graduate Theses and Dissertations

Nitrate (NO3) loadings from stormwater runoff promote eutrophication in surface waters. Low Impact Development (LID) is a type of best management practice aimed at restoring the hydrologic function of watersheds and removing contaminants before they are discharged into ground and surface waters. Also known as rain gardens, a bioretention system is a LID technology that is capable of increasing infliltration, reducing runoff rates and removing pollutants. They can be planted with visually appealing vegetation, which plays a role in nutrient uptake. A modified bioretention system incorporates a submerged internal water storage zone (IWSZ) that includes an electron donor to support …

Synthesis Of [1,2,4]-Triazines As Kinase Inhibitors And Of Novel Fluorine Capture Reagents For Pet Probes, Fenger Zhou

USF Tampa Graduate Theses and Dissertations

ABSTRACT

Anaplastic lymphoma kinase (ALK) is a tyrosine kinase receptor, which plays a pivotal part in the development of the central nervous system. Aberrant expression of full-length ALK occurs in neuroblastoma and chromosomal translocation or inversion of the ALK gene can generate novel fusion-ALK proteins that possess constitutive kinase activity and contribute to oncogenic processes. One of the well-studied fusion proteins is nucleophosmin (NPM-ALK), which draws a lot of attention for medicinal chemists to design small molecules as kinase inhibitors for this target. In this dissertation, [1, 2, 4]-Dihydrotriazine dimers as competitors of the lead compound NVP-TAE684 targeting NPM-ALK have …

Use Of System Dynamics Modeling To Explicate The Theory-Of-Change Of A Social Marketing Innovation, Brian J. Biroscak

USF Tampa Graduate Theses and Dissertations

Community coalitions are an important part of the public health milieu and thus subject to many of the same external pressures as public health organizations--including changes in required strategic orientation. Many funding agencies have shifted their funding agenda from program development to policy change. Thus, the Florida Prevention Research Center created the Community-Based Prevention Marketing for Policy Development framework to teach community coalitions how to apply social marketing to policy change. The dissertation research reported here was designed to explicate the framework's theory-of-change. The research question was: "What are the linkages and connections between CBPM inputs, activities, immediate outcomes, intermediate …

Medical Pluralism In A Neoliberal State: Health And Deservingness In Southern Belize, Douglas Carl Reeser

USF Tampa Graduate Theses and Dissertations

This ethnography explores the varied contours of a national health care system and how it is used in conjunction with traditional forms of health care in Toledo District, Belize, focused on the largest town of Punta Gorda (P.G.), In a medically plural environment, a variety of health care options are used based on a wide range of social, economic, and structural factors that shape people's choices and decisions. The convenience of and experience with low-cost home- and self-care options make these the most common first choice during an illness event in P.G., however a deeper exploration of health behavior reveals …

Novel Models And Efficient Algorithms For Network-Based Optimization In Biomedical Applications, Seyed Javad Sajjadi

USF Tampa Graduate Theses and Dissertations

We introduce and study a novel graph optimization problem to search for multiple cliques with the maximum overall weight, to which we denote as the Maximum Weighted Multiple Clique Problem (MWMCP). This problem arises in research involving network-based data mining, specifically, in bioinformatics where complex diseases, such as various types of cancer and diabetes, are conjectured to be triggered and influenced by a combination of genetic and environmental factors. To integrate potential effects from interplays among underlying candidate factors, we propose a new network-based framework to identify effective biomarkers by searching for "groups" of synergistic risk factors with high predictive …

More To Love: Obesity Histories And Romantic Relationships In The Transition To Adulthood, Hilary Morgan Dotson

USF Tampa Graduate Theses and Dissertations

Previous research suggests that obesity can be stigmatizing in interpersonal relationships, including romantic relationships. Timing of obesity and weight stability are also especially important. The negative effects of obesity on interpersonal relationships appear most salient in women and Whites, while men and racial/ethnic minorities appear to experience fewer negative consequences from obesity in their relationships, suggesting that an intersectional lens is necessary in studies on the long-term effects of obesity on interpersonal relationships. In this dissertation, I employ an intersectional lens to understand how histories of obesity, gender, and racial/ethnic identity work together to influence three aspects of romantic relationships …

The Effects Of U.S. Middle East Foreign Policy On American Muslims: A Case Study Of Muslims In Tampa Bay, Mark G. Grzegorzewski

USF Tampa Graduate Theses and Dissertations

Over the past thirteen years the United States has used military force against three different Muslim-majority nations. These conflicts have lead to the deaths of many Muslims, including many innocent civilians. Meanwhile, American Muslims have become conflicted about their identities as Muslims and Americans. However, this does not mean that they have become a fifth column within America. What it does mean is that they have felt anguish regarding the torment of their religious brethren, while at the same time retaining their American identity. Post-9/11, Muslim American groups have acknowledged their place in the racial ordering of America. Muslim Americans …

The Problems And Potentials In Haunted Maternal Horror Narratives, Sarah Laura Novak

USF Tampa Graduate Theses and Dissertations

This thesis will examine the representation of motherhood in horror cinema in order to discuss the problems and potentials of repeated domestic traditions. While maternal horror narratives impose gender roles based on heterosexual hegemonic biases, some of these films also examine the feminine experience and criticize the patriarchal institutional structures that affect domesticity and femininity. If we discuss these promising features, we can build on the implied trajectories, and engender more representation of marginalized experience in order to seek out new methods of cultural stabilization and unity. This proposal relies on Jacques Derrida's theory of hauntology, which addresses past and …

Inter-Organizational Social Network Information Systems: Diagnosing And Design, Matthew T. Mullarkey

USF Tampa Graduate Theses and Dissertations

While IS research into on-line Inter-Personal (IP) Social Networks (SN) is highly visible, there has been surprisingly little focus on the use of on-line social networks for Inter-Organizational (IO) communications, interactions, and goal achievement. We explore the issues and challenges facing organizations in their design and use of inter-organizational social network information systems (IO SNIS). Artifact design principles are drawn from a new and insightful model that contrasts the advantages of existing innovative inter-personal (IP) SNIS artifacts with Social Network Theory on differences between IP and IO Social Networks. This research extends the existing streams of IS social networking research …

A Multi-Proxy Approach To Understanding Abrupt Climate Change And Laurentide Ice Sheet Melting History Based On Gulf Of Mexico Sediments, Clare Carlisle Williams

USF Tampa Graduate Theses and Dissertations

During the last deglaciation (ca. 24-10 ka thousand years ago (ka)), the North American Laurentide Ice Sheet (LIS) was a major source of meltwater to the Arctic Ocean, North Atlantic Ocean, and the Gulf of Mexico (GOM), and it is hypothesized that meltwater routing played an important role in regulating Late Quaternary millennial-scale climate variability, via its influence on Atlantic Meridional Overturning Circulation (AMOC). For example, the meltwater routing hypothesis predicts that a rerouting of meltwater from the GOM to the North Atlantic and/or Arctic Oceans resulted in a decrease of North Atlantic Deep Water (NADW) formation and subsequent cooling …

Biomaterial Testing Methodology For Long-Term In Vivo Applications: Silicon Carbide Corrosion Resistance, Biocompatibility And Hemocompatibility, Maysam Nezafati

USF Tampa Graduate Theses and Dissertations

Biomedical devices that function in-vivo offer a tremendous promise to improve the quality of life for many who suffer from disease and trauma. The most important consideration for these devices is that they interact with the physiological environment as designed without initiating a deleterious inflammatory response. ISO 10993 outlines the current international guideline for investigating the biocompatibility of such devices. Numerous groups report the use of ISO 10993 as the basis for their experimental evaluation of candidate materials for neuroprosthetics, as well as other biomedical devices, however most of these reports fail to completely comply with the standard. This leads …

Study Of Process Control Strategies For Biological Nutrient Removal In An Oxidation Ditch, Leslie Ann Knapp

USF Tampa Graduate Theses and Dissertations

Advanced wastewater treatment plants must meet permit requirements for organics, solids, nutrients and indicator bacteria, while striving to do so in a cost effective manner. This requires meeting day-to-day fluctuations in climate, influent flows and pollutant loads as well as equipment availability with appropriate and effective process control measures. A study was carried out to assess performance and process control strategies at the Falkenburg Road Advanced Wastewater Treatment Plant in Hillsborough County, Florida.

Three main areas for control of the wastewater treatment process are aeration, return and waste sludge flows, and addition of chemicals. The Falkenburg AWWTP uses oxidation ditches …

Paradise Always Already Lost: Myth, Memory, And Matter In English Literature, Elizabeth Stuart Angello

USF Tampa Graduate Theses and Dissertations

This dissertation follows a collection of agentive objects around and through the networks of humans and nonhumans in four disparate works of English literature: the Anglo-Saxon poem The Dream of the Rood, William Shakespeare's narrative poem The Rape of Lucrece, Thomas Hardy's novel The Woodlanders, and Philip Pullman's trilogy His Dark Materials. Applying the emergent discourses of object-oriented analyses, I posit the need for a critique that considers literary objects not as textual versions of real-world objects but as constructs of human imagination. What happens when we treat nonhuman or inanimate objects in literature as full …

The Interrelationship Of Brca1 185delag, Interleukin-1Β, And Ovarian Oncogenesis, Kamisha Woolery

USF Tampa Graduate Theses and Dissertations

While the etiology of ovarian cancer (OC) is not completely understood, evidence suggests that chronic inflammation may promote malignant transformation. However, familial history remains the strongest risk factor for developing OC and is associated with germline BRCA1 mutations, such as the 185delAG mutation. Normal human ovarian surface epithelial cells expressing the 185delAG mutant, BRAT, exhibit molecular and pathological changes that may contribute to OC oncogenesis. In the current study, I sought to determine whether BRAT could promote an inflammatory phenotype by investigating BRAT's impact on the expression of the proinflammatory cytokine, Interleukin-1β (IL-1β). Using a culture model system of normal …

Manipulating The Tumor Microenvironment For Therapeutic Benefit, Kate M. Bailey

USF Tampa Graduate Theses and Dissertations

The physical tumor microenvironment contributes significantly to carcinogenesis, cancer progression and metastatic dissemination. Two main components of the tumor microenvironment, hypoxia and acidosis, are present in nearly every solid tumor and act as powerful selection forces against the tumor. Hypoxia and acidosis promote tumor heterogeneity and contribute to chemotherapy and radiotherapy resistance. This dissertation interrogates methods to target the tumor microenvironment including two novel studies describing mechanisms of buffer therapy resistance and targeting tumor hypoxia with vasodilators to enhance the efficacy of a hypoxia activated prodrug, TH-302.

In the first study, mechanisms of buffer therapy resistance were identified and detailed. …

Evolution And Equilibration Of Artificial Morphologic Perturbations In The Form Of Nearshore Berm Nourishments Along The Florida Gulf Coast, Katherine Emily Brutsché

USF Tampa Graduate Theses and Dissertations

Inlets and channels are dredged often to maintain navigation safety. It is beneficial to reintroduce the dredged material back into the littoral system, in the form of beach or nearshore nourishments. Nourishment in the nearshore is becoming an increasingly utilized method, particularly for dredged material that contains more fine sediment than the native beach. This research examines the morphologic evolution of two different nearshore nourishments. A nearshore berm was constructed at Fort Myers Beach, Florida using mixed-sized sediment dredged from a nearby channel. The nearshore berm was placed in water depths between 1.2 and 2.4 m with the berm crest …

Just Love: A Collaborative Evaluation Of A Faith-Based School-Family-Community Partnership Through The Voices Of The Children, Lynette M. Henry

USF Tampa Graduate Theses and Dissertations

Abstract

Faith-based school-family-community partnerships have been a federal mandate over the past decade, yet little has been written about the outcomes of these faith-based partnerships. A need exists to understand if the potential in these faith-based partnerships is indeed realized in positive outcomes for students and schools. The purpose of this study was to evaluate a faith-based school-family-community partnership, Just Love. Just Love is a faith-based school-family-community partnership between a large suburban church and a Title 1, urban elementary school, Charisma Elementary School (Charisma ES; pseudonym). It was implemented in what is considered a "failing school". Just Love's purpose was …

Cancer And Infection, Kathleen Hope Plummer

USF Tampa Graduate Theses and Dissertations

E. coli is the most frequently isolated Gram negative pathogen from bacteremia in cancer patients and is repeatedly recovered from many other extraintestinal illnesses. These infections are commonly endogenous in nature and interfere with the treatment of cancer resulting in increased healthcare costs, morbidity, and mortality rates. Cancer and the treatments related to cancer cause alterations in the microbiome of the gut and other organs. Despite this point, there is a serious lack of knowledge about the genetic types of E. coli infecting cancer patients. This gap results in vague prevention strategies and limited treatment options for cancer patients. Multi …

The Development And Initial Validation Of An Instrument Measuring The Cognitive Domain Of Intercultural Maturity, Melanie Lynne Wicinski

USF Tampa Graduate Theses and Dissertations

Abstract

Through a two-phase process an instrument was created to measure the cognitive domain as proposed by King and Baxter Magolda in their Developmental Model of Intercultural Maturity (2005). The first phase involved expert panels who identified the competencies which exist in the cognitive domain, identified situations which might exist between individuals from different cultures, validated scenarios created from the identified situations, and created responses which corresponded to the three developmental levels (Initial, Intermediate, and Mature) defined in the Developmental Theory of Intercultural Maturity.

Within the second phase, the created instrument was administered to 371 individuals representing eight geocultural world …

Rna Detection Technology For Applications In Marine Science: Microbes To Fish, Robert Michael Ulrich

USF Tampa Graduate Theses and Dissertations

The accurate identification of taxa from mixed assemblages using genetic analysis remains an important field of molecular biology research. The common principle behind the development of numerous documented genetic detection technologies is to exploit specific nucleotide sequences inherent to each taxon. This body of work focuses on practical applications of real-time nucleic acid sequence-based amplification (RT-NASBA) in marine science, and is presented in four case studies. Each study represents novel work in the genetic identification of respective taxa of interest using RT-NASBA. Two case studies documented the development of an assay targeting mitochondrial 16S rRNA to discern legally salable grouper …