Digital Commons Network^™

Dynamics And Survival Of Coral And Octocoral Juveniles Following Disturbance On Patch Reefs Of The Florida Reef Tract, Lucy Bartlett

USF Tampa Graduate Theses and Dissertations

Over the past several decades, rapid decline in adult stony-coral (comprising the Orders Scleractinia and Anthomedusae, specifically Family Milleporidae) cover has occurred concurrent with an increase in adult octocoral (Octocorallia/gorgonian) cover along the Florida Reef Tract. In January 2010, the Florida Keys experienced extremely cold air and water temperatures, below the lethal threshold for many reef organisms including corals. Very high stony-coral mortality occurred on some patch reefs. The newly-available space created by this disturbance event provided the opportunity for recruitment and settlement of new coral larvae and other reef organisms.

The goal of this study was to examine post-disturbance …

An Exploration Of The Health Experiences Of Youth Who Were Trafficked For Sex, Christine Ann Meister

USF Tampa Graduate Theses and Dissertations

This pilot study used photovoice methodology to explore the current health needs of youth who were trafficked for sex and are now living in an alternative family care setting. The goals of this study were to add to the current literature regarding the health needs of victims of domestic minor sex trafficking and to add a qualitative aspect to the evaluation of the Citrus Helping Adolescents Negatively Impacted by Commercial Exploitation (CHANCE) program. Disposable cameras were distributed to a group of adolescent girls enrolled in the program who were then tasked with taking pictures of things that made them feel …

Groundwater Controls On Physical And Chemical Processes In Streamside Wetlands And Headwater Streams In The Kenai Peninsula, Alaska, Michael Kroh Callahan

USF Tampa Graduate Theses and Dissertations

For this dissertation I studied groundwater and surface water interactions in the Kenai Lowlands, Alaska. In particular, I examine two important aspects of groundwater and surface water interactions: 1) Groundwater's influence on surface-water temperature; and 2) Groundwater's role in forming hydrologic flow paths that can connect uplands to streamside wetlands and streams. Chapter 2 investigates the controls on stream temperature in salmon-bearing headwater streams in two common hydrogeologic settings: 1) drainage-ways, which are low-gradient streams that flow through broad valleys; and 2) discharge-slopes, which are high gradient streams that flow through narrow valleys. The results from chapter 2 showed significant …

Assessments Of Surface-Pelagic Drift Communities And Behavior Of Early Juvenile Sea Turtles In The Northern Gulf Of Mexico, Robert F. Hardy

USF Tampa Graduate Theses and Dissertations

Knowledge of species distribution and habitat associations are essential for conservation measures. Such information is lacking for many marine species due to their occupancy of broad and ephemeral habitats that are difficult to access for study. Sea turtles, specifically the surface-pelagic juvenile stage of some species, are a group for which significant knowledge gaps remain surrounding their distribution and habitat use. Recent research has confirmed the long-standing hypothesis that the surface-pelagic juvenile stage occurs within surface-pelagic drift communities (SPDC). Within the North Atlantic and surrounding basins, the holopelagic macroalgae Sargassum spp. dominates SPDC and serves as a remotely-detectable indicator of …

Human Mirna Sequence Based Variations Database, Nadim Georges Bou Zeidan

USF Tampa Graduate Theses and Dissertations

MicroRNAs (miRNAs) are studied as key genetic elements that regulate the gene expression involved in different human diseases. Clinical sequence based variations like copy number variations (CNVs) affect miRNA biogenesis, dosage and target recognition that may represent potentially functional variants and relevant target bindings.

To systematically analyze miRNA-related CNVs and their effects on related genes, a user-friendly free online database was developed to provide further analysis of co-localization of miRNA loci with human genome CNV regions. Further analysis pipelines such as miRNA-target to estimate the levels or locations of variations for genetic duplications, insertions or deletions were also offered. Such …

Distribution Of Dissolved Trace Metals And Carbon System Parameters In The Surface Waters Of The Hillsborough River And Tampa Bay, Matthew Matthias Elliott

USF Tampa Graduate Theses and Dissertations

This work represents a first look at the general spatial distribution of trace metals and the inorganic carbon system in the surface waters of Tampa Bay. Tampa Bay's chemical environment bears many influences ranging from hydrological and geological to anthropogenic and meteorological. A large y-shaped estuary on the west-central side of the Florida Peninsula, Tampa Bay extends over 400 square miles and has a 2200 square mile watershed that includes extensive swamps, scrub, agricultural lands and densely urbanized areas. Reaching 37 miles from the northernmost point of Old Tampa Bay to the mouth of the estuary near the Sunshine Skyway …

Operation Optimization And Water Quality Simulation Of Potable Water Distribution System, Xiongfei Xie

USF Tampa Graduate Theses and Dissertations

A potable water distribution system (WDS) consists of pipes, pumps, valves, storage tanks, control and supporting components. Traditionally, it has two basic functions. First, provides end users with potable water at sufficient pressures and good water quality. Second, provides sufficient pressure and flow for fire fighting. Currently, potable water is still the least expensive material for fire fighting. To accomplish these two goals, water utilities have to consider the integrity and security of the water network. As a result, this research selected three research topics that are closely related to the daily operation of water utilities and water quality simulation. …

Novel Therapeutic Strategies In Lung Cancer, Courtney A. Kurtyka

USF Tampa Graduate Theses and Dissertations

Lung cancer is the leading cause of cancer-related death and the second most diagnosed cancer in the United States. Unfortunately, many patients either do not have any common mutations for which there are already targetable agents, or they eventually become resistant to these compounds. As such, there is a high demand for new, effective methods of treating this disease as well as predicting patient prognosis and potential benefit from chemotherapy. In this work, numerous strategies for treating lung cancer are explored.

The first method described here is through the use of a pan-early 2 factor (E2F) inhibitor, HLM006474, which is …

Differentiating Geo-Spatiotemporal Aquatic Larval Habitats Of Anopheles Gambiae Complex In Urban Agriculture And Urban Non-Agriculture Environments In Accra, Ghana, Samia Mckeever

USF Tampa Graduate Theses and Dissertations

To meet the rising food demands of communities in Accra,Ghana, urban agriculture has been popularized as a way to increase food security and improve nutrition (Donovan et al., 2012). Urban agriculture is defined as "the cultivation of crops at both the subsistence and commercial levels including the keeping of livestock in open spaces in urban areas (Adjaye, n.d.). In Accra, urban agriculture covers 1,091 hectares, employs over one thousand people, and supplies residents with 90% of its vegetables ("Accra Metropolitan", n.d.). Further, 60% of households in Accra participate in backyard farming ("Accra Metropolitan", n.d.). Although urban agriculture provides many benefits …

Investigation Of Enhanced Titanium And Zinc Oxide Semiconductors For The Photodegradation Of Aqueous Organic Compounds, Innocent Udom

USF Tampa Graduate Theses and Dissertations

Growing demand and shortages of potable water sources due to industrialization have become a great concern worldwide. Various approaches and solutions have been adopted to provide cleaner and quality water. In a preliminary study, a method of treating wastewater was investigated in which algae were used to remove nutrients (nitrogen and phosphorous) from wastewater and then the algae were harvested for use as a biofuel. The results from this investigation are included in the Appendix B. Employing traditional oxidants, such as hydrogen peroxide, chlorine, and ozone, for treatment of recalcitrant organic compounds have achieved less promising results. However, photocatalysis, an …

Understanding Factors Determining Early Termination From A Government Assistance Program For Maternal And Child Health: The Special Supplemental Nutrition Program For Women, Infants And Children (Wic), Anthony Dominic Panzera

USF Tampa Graduate Theses and Dissertations

The purpose of this dissertation is to understand why individuals enrolled in the Special Supplemental Nutrition Program for Women, Infants and Children (WIC) fail to retrieve food vouchers, miss WIC appointments, and become inactive in program components. In Kentucky, mothers who fail to pick up food instruments for 60 days are automatically terminated from the program. The specific research questions that guided this study are: (1) Which segments of enrollees are at greatest and least risks of nonparticipation in the WIC program? (2) How do predisposing, enabling and need characteristics impact WIC nonparticipation among eligible mothers? (3) How do WIC …

Mechanisms And Molecular Biology Of Major Tumor Suppressors, Brienne E. Engel

USF Tampa Graduate Theses and Dissertations

This dissertation is devoted to the study of the molecular biology of major tumor suppressors, defined as those that prevent the cellular processes identified as the hallmarks of cancer. Specifically, the major tumor suppressors pRb and STK11 are explored in the context of osteosarcoma and lung cancer, respectively.

RB1 was the first tumor suppressor gene discovered. Over four decades of work have revealed that the Rb protein (pRb) is a master regulator of biological pathways influencing virtually every aspect of intrinsic cell fate including cell growth, cell-cycle checkpoints, differentiation, senescence, self-renewal, replication, genomic stability and apoptosis. While these many processes …

Role And Regulation Of Snon/Skil And Plscr1 Located At 3q26.2 And 3q23, Respectively, In Ovarian Cancer Pathophysiology, Madhav Karthik Kodigepalli

USF Tampa Graduate Theses and Dissertations

Ovarian cancer is one of the most common causes of gynecological cancer related deaths in women. In 2014, the estimated number of deaths due to ovarian cancer is 14,270 with occurrence of over 22, 240 new cases (National Cancer Institute, http://seer.cancer.gov/statfacts/html/ovary.html). Despite improvement in treatment strategies, the 5-year survival rate is still below 50% mainly due to chemoresistance and relapse. Amplification of chromosomal region 3q26 is a common characteristic in various epithelial cancers including ovarian cancer. This region harbors various oncogenes including the TGFβ signaling mediators EVI1 and SnoN/SkiL, PKCι and PIK3CA amplified at 3q26.2 and 3q26.3, respectively, in ovarian …

Eukaryotic Gene Expression Patterns Of Microorganisms In The Amazon River Plume Parallel The Biogeochemistry Of Plume Waters, Brian L. Zielinski

USF Tampa Graduate Theses and Dissertations

Unraveling the microbiological processes that occur as water travels from a river's mouth into the ocean is critical to understanding the role of river plumes in global biogeochemical cycles. Metranscriptomics, the gene expression of a whole community of organisms, was utilized to examine six stations along the Amazon River Plume (ARP) in 2010 to test thehypothesis that there were measurable differences in gene expression for key biogeochemical genes along the ARP. This body of work focuses on methods developed to identify which genes are biogeochemically important for a particular environment along extreme salinity, nutrient and community gradients in the ARP, …

Developmental Trajectories Of Physical Aggression And Nonaggressive Rule-Breaking Among At-Risk Males And Females During Late Childhood And Early Adolescence, Eugena Givens

USF Tampa Graduate Theses and Dissertations

Criminological, psychological, and developmental researchers have relentlessly explored behavioral characteristics and juvenile justice outcomes in an effort to establish the most appropriate means of analyzing childhood and adolescent problem behaviors. Cross-discipline, empirical evidence and factor analytic research has consistently identified the presence of two predictive concepts, physical aggression and nonaggressive rule-breaking. Research pertaining to the risk factors and correlates of these two distinct substructures of offending align with theoretically postulated typologies of delinquency and offending as well as the frequently cited patterns of delinquency and offending within reviews of longitudinal research. Using longitudinal data from a sample of 756 at-risk, …

Risk Perception And Beliefs About Volcanic Hazards: A Comparative Study Of Puna District Residents, Melanie Marie Leathers

USF Tampa Graduate Theses and Dissertations

The purpose of this research is to better understand how residents of communities located on the flanks of Kilauea, Hawai'i view the hazards associated with volcanic events taking into account hazard proximity, cultural beliefs, municipal trust, and evacuation planning. The study was conducted in the lower Puna district, an area with a rapidly growing population but limited infrastructure.

Data were collected though a questionnaire survey undertaken at venues throughout the district, including grocery markets, bakeries, farmers markets, the public pool, and other gathering places. Overall, the results indicated that people understand the natural hazards of the place but are generally …

Karst Estuaries: A Newly Described Ecosystem Governed By Aquifer Hydrology, Damian M. Menning

USF Tampa Graduate Theses and Dissertations

The overall goal of this dissertation is to define the hydrological, geochemical, and biological characteristics of a Karst Estuary. These types of estuaries represent a unique ecosystem created by freshwater inputs from direct flow through karst conduits and/or diffuse flow through a karst matrix. In order to determine the characteristics of a Karst Estuary we monitored short-term tidal fluctuations, long-term rainfall patterns, aquifer levels, spring discharge, multiple geochemical parameters, microbial communities in the water column and sediment, and macrofaunal communities in the sediment along a transect from a submarine spring through the Gulf of Mexico. Four sites were selected along …

The Importance Of Family-Systems Theory In Masters-Level School School Counseling Curriculum: A Study Of Faculty Perceptions, Gwen Gold

USF Tampa Graduate Theses and Dissertations

This study examined the perceptions of Council for Accreditation of Counseling & Related Educational Programs (CACREP) master's-level school counseling program coordinators and faculty members about the importance and relevance of family systems theory and techniques coursework in their program curriculum. Family-systems theory coursework is lacking in two-thirds of those accredited programs, although mandated by their major accrediting body, CACREP. CACREP issued curriculum standards and guidelines to ensure proper training of school-counseling students and the American School Counselor Association--the foundation that expands the image and influence of professional school counselors through advocacy, leadership, collaboration, and systemic change--has issued guidelines for the …

The Tattoo: A Mark Of Subversion, Deviance, Or Mainstream Self-Expression?, Jocelyn Camacho

USF Tampa Graduate Theses and Dissertations

While an estimated one-third of the United States population has a tattoo, tattoos are still seen as a sign of deviance. The appearance of the first tattoos in the United States were relegated to the bodies of the lower classes and outcasts of society. Over the past few decades tattoos have migrated on to the celebrity skin of today's pop culture icons. In the past twenty years, tattoos have moved from deviant subcultures to the mainstream, and yet are still considered to be a mark of the disfavored factions of society. The dominant culture continues to regard the bearers of …

Portable X-Ray Fluorescence Analysis Of Pottery At The Bayshore Homes Site In Pinellas County, Florida, Rachel Nostrom

USF Tampa Graduate Theses and Dissertations

The Bayshore Homes site was occupied intermittently over a period of approximately twelve hundred years, with the two main occupation periods being CE 150-550 and CE 900-1350. During those lengthy occupations a substantial amount of plain and decorated pottery was discarded at the site. A portable X-ray fluorescence (pXRF) spectrometer was utilized to analyze the elemental composition of 133 sherds, both decorated and plain. The resulting elemental composition data were then analyzed using multivariate statistics in an attempt to discern discrete clay sources that may have been exploited by inhabitants of the Bayshore Homes site. Principal component analysis (PCA) and …

Accuracy Of /T/ Productions In Children With Cochlear Implants As Compared To Normal-Hearing, Articulation Age-Matched Peers, Terry Gier

USF Tampa Graduate Theses and Dissertations

Children who receive cochlear implants (CIs) demonstrate considerable variability in speech sound production. Investigations focused on speech sound development in children with CIs have shown initial accelerated growth, followed by a plateau where consonant order of acquisition generally mirrors that of NH children, but is slower (Blamey, Barry, & Pascale, 2001; Serry & Blamey, 1999; Spencer & Guo, 2013). A notable exception to this pattern, /t/, has been shown to be acquired later-than normal in several investigations (Blamey et al., 2001; Chin, 2003; Ertmer, True Kloiber, Jongmin, Connell Kirleis, & Bradford, 2012). The primary purpose of this investigation was to …

Ontogenetic Diet Shifts And Prey Preference Of A Generalist Predatory Fish, Brittany Jalene Scharf

USF Tampa Graduate Theses and Dissertations

Marine ecosystems are highly dynamic and contain a diverse faunal assemblage that are subject to various natural and anthropogenic variability. Globally, seagrass ecosystems are located adjacent to coastal areas that are heavily impacted by human development and urbanization potentially altering the community structure within these ecosystem. Complex food webs connect many components of these systems, often in unexpected ways, and are ultimately based on one of two pathways: benthic (i.e., seagrass, epiphytes, microalgae, detritus) and planktonic (i.e., phytoplankton). Understanding the pathway which the food web is based gives further insight regarding the biological balance of the ecosystem; thus it is …

Adolescent Behavioral Adjustment In Girls Adopted From China: Examining Pre-Adoption And Post-Adoption Factors, Derek Justin Powers

USF Tampa Graduate Theses and Dissertations

Despite research that indicates that internationally adopted children are at greater risk for poor developmental outcomes than their non-adopted peers (Bimmel, Juffer, IJzendoorn, Bakermans-Kranenburg, 2003; Juffer, & van IJzendoorn, 2005), girls adopted from China into Western culture tend to thrive, exhibiting high self-esteem, low behavior problems (i.e., both externalizing and internalizing), and excelling academically (Rojewski, Shapiro, & Shapiro, 2000; Tan & Jordan-Arthur, 2012). However, few studies have examined whether this trend continues into adolescence, as well as to what factors lead to these positive outcomes. The purpose of this study was to investigate predictors of mental health outcomes among internationally …

Managing Regional Water Resources Amidst Rapid Urbanization In Southwest Florida: A Case Study, Nicole Owusua Caesar

USF Tampa Graduate Theses and Dissertations

Experts and organizations involved in freshwater resources management have emphasized the importance of long-term urban resource planning and management that considers the tight coupling which exists between human - nature - technology systems. The resistance of contemporary urban growth efforts to consider resource carrying capacities and ecosystem requirements has led to costly unintended consequences including the deterioration of natural capital and their associated ecosystem services, and the degradation of water resource flows. As these problems continue to worsen, resource experts have called for the development of a new water resource management paradigm inclusive of various sustainability criteria.

Historically water-rich Florida …

Characterization Of The Depositional Environment Of Phreatic Overgrowths On Speleothems In The Littoral Caves Of Mallorca (Spain): A Physical, Geochemical, And Stable Isotopic Study, Liana Marie Boop

USF Tampa Graduate Theses and Dissertations

Phreatic overgrowths on speleothems (POS) are one of many sea-level proxies available to Quaternary geologists in Mallorca; these carbonate encrustations form at the air-water interface in cave passages flooded with brackish water. POS are ideal for reconstruction of western Mediterranean sea level because they are widespread in Mallorca's caves, can be precisely dated by U-series methods, constrain sea-level stands to sub-meter elevation, and are well preserved and accessible in the subterranean environment.

This research investigates the POS depositional environment, which is relatively understudied compared to the other proxies used for sea-level reconstructions. This disparity has led to assumptions on many …

Risk Of Compliance: Tracing Safety And Efficacy In Mef-Lariam's Licensure, Julie Marie Gerdes

USF Tampa Graduate Theses and Dissertations

The Walter Reed Institute of Army Research developed the antimalarial drug mefloquine then collaborated with Hoffman-La Roche to produce the drug under its brand name "Lariam," after Food and Drug Administration (FDA) approved licensure in 1989. For over twenty years, the Army used this pill as its "drug of choice" for soldiers deployed to endemic regions until 2009, and in 2013 the Food and Drug Administration warned that the drug's neurotoxic effects could be lasting, if not permanent. The sociopolitical exigence of developing a new biochemical antimalarial drug rushed the development and licensure processes, and the modern craving for certainty …

Statistical Modeling And Prediction Of Hiv/Aids Prognosis: Bayesian Analyses Of Nonlinear Dynamic Mixtures, Xiaosun Lu

USF Tampa Graduate Theses and Dissertations

Statistical analyses and modeling have contributed greatly to our understanding of the pathogenesis of HIV-1 infection; they also provide guidance for the treatment of AIDS patients and evaluation of antiretroviral (ARV) therapies. Various statistical methods, nonlinear mixed-effects models in particular, have been applied to model the CD4 and viral load trajectories. A common assumption in these methods is all patients come from a homogeneous population following one mean trajectories. This assumption unfortunately obscures important characteristic difference between subgroups of patients whose response to treatment and whose disease trajectories are biologically different. It also may lack the robustness against population heterogeneity …

Development Of An Efficient Human Hepatocyte Culture Platform For Assessing Novel Therapeutic Efficacy Against Plasmodium Liver Parasites, Steven Patrick Maher

USF Tampa Graduate Theses and Dissertations

Malaria is a critical and global public health problem, affecting over 200 million people every year, resulting in over 500,000 deaths. A vaccine is not currently available and only one drug, primaquine, is effective against the dormant stages of Plasmodium vivax. Preclinical assessment of novel therapeutic drugs and vaccines is hampered by the lack of an in vitro liver model for P. falciparum and P. vivax. To provide a stable human hepatocyte-based culture platform for parasite development, we engineered a microfluidic bilayer device capable of both simple and complex culture methods, including perfusion and co-culture, to better understand the requirements …

Chiral Phosphoric Acids And Alkaline Earth Metal Phosphates Chemistry, Tao Liang

USF Tampa Graduate Theses and Dissertations

Asymmetric synthesis and catalysis is one of the leading research areas in chemistry society, for its versatility and efficiency in obtaining chiral molecules that found the vast majority in natural active compounds and synthetic drugs. Developing asymmetric catalytic methodology is at the frontier in both industrial and academic research laboratories. Enantioselective organocatalysis has emerged as a powerful synthetic tool that is complementary to metal-catalyzed transformations. The development of chiral phosphoric acid and metal phosphate as catalysts has been a breakthrough in recent years. Chiral phosphoric acids have been shown to be powerful catalysts in many organic transformations. Moreover, chiral metal …

Connecting Institutional Discourses And Everyday Understandings Of Climate Change: Viewpoints From A Suburban Neighborhood In Tampa, Florida, Christopher Metzger

USF Tampa Graduate Theses and Dissertations

Despite a general consensus regarding anthropogenic global climate change across the international scientific community, many of the major greenhouse gas producers in the world, especially the United States, are hesitant to implement strict emissions regulations. According to some prominent atmospheric scientists, such as James Hansen and Michael Mann, if industrialized countries continue to produce carbon emissions at current rates, an irreversible planetary tipping point of raising temperatures 2°C above pre-industrial levels could be reached in less than 40 years. Societies have a wealth of information from the natural sciences to understand the climate problem and currently possess the technological means …