Digital Commons Network^™

Development Of A Reusable Nutrient Recovery System To Sustainably Integrate Small-Scale Sanitation And Agriculture, Jorge Luis Calabria

USF Tampa Graduate Theses and Dissertations

Emerging technologies in sanitation and food production foster the potential to advance progress towards key sustainable development goals of ensuring adequate sanitation and food security to all. Non-Sewered Sanitation Systems (NSSSs), as detailed by the recent International Organization for Standardization’s (ISO) 30500 standard, are being developed to overcome challenges of lacking sanitation infrastructure and resource scarcity by providing autonomous, off-grid wastewater treatment (WWT) service in a much smaller footprints than large centralized treatment facilities. A promising NSSS platform is the anaerobic membrane bioreactor (AnMBR) that can achieve high (WWT) throughput in a small footprint using ultrafiltration (UF) membrane filtration to …

A Functional Approach To Resolving The Biogeocomplexity Of Two Extreme Environments, Haydn Rubelmann Iii

USF Tampa Graduate Theses and Dissertations

The biodiversity of two distinct marine environments was observed to describe the biogeocomplexity of these extreme ecological systems. A shallow-water hydrothermal vent in Papua New Guinea served as a study of a thermophilic ecosystem influenced by arsenic rich vent fluids while a 60 m deep offshore primarily anoxic karst sink served as a study of an anaerobic sulfur-influenced habitat. Both environments support unique biological communities that are influenced by the physical and chemical pressures imposed on them by the harsh conditions of these systems. In Tutum Bay, Ambitle Isle, Papua New Guinea, a transect was created from a shallow hydrothermal …

Investigations Of The Physical And Analytical Chemistry Of Iron In Aqueous Solutions, James Patten

USF Tampa Graduate Theses and Dissertations

Although iron occurs at extremely low concentrations in the world’s oceans, it is essential for all living organisms. It is the limiting nutrient in High Nutrient Low Chlorophyll (HNLC) areas of the ocean, and exerts critically important influences on levels of atmospheric CO₂ and the global carbon cycle. Understanding the chemical processes that govern the fluxes and biogeochemistry of oceanic iron requires thorough assessment of the aqueous physical chemistry of iron and analytical techniques capable of measuring iron at sub-nanomolar concentration measurements. This dissertation extends prior work on the physical and analytical chemistry of iron through (a) investigation of …

Statistical Analysis And Modeling Of Pm2.5 Speciation Metals And Their Mixtures, Boubakari Ibrahimou

USF Tampa Graduate Theses and Dissertations

Exposure to fine particulate matter (PM_2.5) in the ambient air is associated with various health effects. There is increasing evidence which implicates the central role played by specific chemical components such as heavy metals of PM_2.5. Given the fact that humans are exposed to complex mixtures of environmental pollutants such as PM_2.5, research efforts are intensifying to study the mixtures composition and the emission sources of ambient PM, and the exposure-related health effects. Factor analysis as well source apportionment models are statistical tools potentially useful for characterizing mixtures in PM_2.5. However, classic …

Predicting Adolescents' Academic Achievement: The Contribution Of Attention And Working Memory, Diane Elizabeth Napier

USF Tampa Graduate Theses and Dissertations

The present study examined the direction and strength of the relation between three different areas academic achievement and working memory with adolescent students. The data analyzed included ratings for inattention, a diagnosis of ADHD (or not), and demographic information for race/ethnicity. Fifty children aged 11 to16 years of age participated in the study. Participants were recruited from several middle schools, homeschooling networks, and churches from a southeastern state of the United States. Each participant completed a standardized achievement test, a behavioral rating scale, and visual and verbal working memory tests. The research questions investigated: 1) the relation between visual and …

Short- And Long-Term Trends In Ecological Interactions: From Predator-Prey Interactions To Phanerozoic Diversification, Subhronil Mondal

USF Tampa Graduate Theses and Dissertations

During the last two decades, a broad spectrum of short- and long-term studies on different taxonomic groups has enriched our understanding about how dynamics of taxonomic and ecological diversification have changed through geologic time. There are two major issues that have impacted these studies: the quality and quantity of data used are often insufficient in various ways and the methods used may produce results that are more equivocal than supposed. To investigate these issues more fully, this dissertation focuses on studies on two major aspects: 1) short-term studies examining the nature of successful and unsuccessful predatory attacks on Plio-Pleistocene bivalves; …

Functional Morphology And Feeding Mechanics Of Billfishes, María Laura Habegger

USF Tampa Graduate Theses and Dissertations

Billfishes (marlins, spearfishes, sailfishes and swordfish) are one of the fastest and largest marine apex predators, and perhaps their most recognizable attribute is their bill or rostrum. The proposed function for this novel structure has ranged from hydrodynamic enhancement to defensive weaponry. However, the most supported hypothesis for its function has been linked to feeding. Billfishes have been observed to subdue their prey with their rostrum, either stunning or cutting them into pieces before ingestion. Due to their large body sizes and pelagic lifestyles a thorough investigation of the function of this structure has been logistically challenging. The goal of …

Computer-Aided Structure-Based Drug Discovery: Cxcl12, P. Aeruginosa Lpxa, And The Tiam1 Pdz Domain, Emmanuel William Smith

USF Tampa Graduate Theses and Dissertations

For structure-based drug discovery, structural information of a target protein is necessary. NMR, or X-ray crystallography can provide necessary information on active site configuration that can lead a successful virtual screening campaign into identifying binders that may then be optimized into potent inhibitors. However, many challenges exist in the structure-based drug discovery cycle. For instance, structure determination of a protein of interest can many times be a daunting task. In addition, complex structure determination, which can allow essential characterization of protein-ligand interactions, is also challenging and many times impossible. Virtual screening heavily relies on such structural information, but hit-to-lead optimization …

Influences Of Yard Management Intensity On Urban Soil Biogeochemistry, Viviana Penuela Useche

USF Tampa Graduate Theses and Dissertations

Soils are critical to ecosystem function as they provide essential nutrients for primary producers, habitat and organic energy for decomposers, and storage of organic matter. Irrigation with reclaimed water is an increasingly popular water conservation strategy; yet its high salinity and nutrient content potentially affect soil properties. In this study, set in a residential neighborhood of Tampa (U.S.). I tested whether there are distinct lawn system management strategies characterized by systematic differences in reclaimed water usage and irrigation and fertilization practices. I then investigated whether soil biogeochemistry responds to lawn system management strategy.

My results indicated that amendment strategy, which …

Examining Activity Organization In Plazas Through Geochemical Analysis At Tlalancaleca, Puebla, Mexico (800 Bc-Ad 100), Paige Gale Phillips

USF Tampa Graduate Theses and Dissertations

This research aims to understand the organization of activities across a prehispanic urban center at the Formative period site of Tlalancaleca (800 BC- AD 100), located in Puebla, Mexico. This study analyzes soil samples at the central civic-ceremonial complex of Cerro Grande in an attempt to understand the use of space. This work is a part of the larger Proyecto Arqueológico Tlalancaleca, Puebla (PATP), which is focused on understanding the socio-political organization at Tlalancaleca that led to this site of early urbanism. Soil samples from Tlalancaleca are analyzed using three chemical methods to perform a cross-comparison of analytical methods. These …

Developing An Animal Model Of Polysubstance Abuse In Adolescence: The Role Of Nmda Receptors In Alcohol/Cocaine Reward, Adriana Rebecca Uruena-Agnes

USF Tampa Graduate Theses and Dissertations

Repeated exposure to drugs of abuse conditions individuals to anticipate the behavioral consequences of drug use specifically in the presence of a drug-associated context. In rodents, preferences and aversions for alcohol and cocaine have been conditioned; however, the mechanisms underlying the expression of these conditioned effects remain unknown. Given that alcohol and cocaine polysubstance abuse is prevalent in young individuals, with more than 50% of these polysubstance abusers reporting to be under the age of 21, it is important to understand the mechanisms contributing to the behavioral effects of alcohol and cocaine co-dependency. Aim 1 determined if age differentially impacted …

Risk Factors For Recurrent Major Depressive Disorder In A Nationally Representative Sample, Graig Charles Defeo

USF Tampa Graduate Theses and Dissertations

The public use version of the National Comorbidity Survey - Replication (NCS-R) dataset was used (N = 995) to investigate risk factors for recurrent major depressive disorder (MDD) that are evident before recovery from the first major depressive episode (MDE) by comparing persons diagnosed with MDD who experienced a single MDE to persons with recurrent MDD.

Multiple logistic regression analyses assessed the independent risk of recurrent MDD for each of the following risk factors: an early age of onset (old), absence of a life stress trigger, chronic first episode, childhood parental loss, parental maltreatment, parental depression, comorbid anxiety disorder, and …

Growth Rates In Gulf Of Mexico Red Snapper, Lutjanus Campechanus, Before And After The Deepwater Horizon Blowout, Elizabeth Shea Herdter

USF Tampa Graduate Theses and Dissertations

The Deepwater Horizon blowout occurred on April 20^th, 2010 and released nearly 5 million barrels of crude oil into the northern Gulf of Mexico causing pollution of the water and sediment inhabited by many fishes for at least 87 days while the wellhead went uncapped. Populations of the Gulf of Mexico Red snapper, Lutjanus campechanus, an important fish to the ecology and economy in the region, exhibit affinity to shallow water oil infrastructure such as the Deepwater Horizon making them especially vulnerable to crude oil contamination. The objective of this study is to determine growth of Red …

Polycyclic Aromatic Hydrocarbon Metabolites As A Biomarker Of Exposure To Oil In Demersal Fishes Following The Deepwater Horizon Blowout, Susan Susan Snyder

USF Tampa Graduate Theses and Dissertations

The Deepwater Horizon blowout occurred on April 20th, 2010, releasing 4.9 million barrels of Louisiana crude oil into the Gulf of Mexico (GoM). Subsequent to the Deepwater Horizon blowout, sediment cores revealed oil on the northern GoM seafloor and abnormal skin lesions were seen in GoM fishes. Exposure to polycyclic aromatic hydrocarbons (PAHs), a component of crude oil, in fish has been associated with many sublethal effects, including cancer and population-level effects. Using a biomarker of exposure to PAHs, this thesis evaluates inter-species, temporal and spatial differences in exposure to hydrocarbon contamination between three species of fish with varying levels …

Use Of A Towed Camera System For Estimating Reef Fish Populations Densities On The West Florida Shelf, Sarah Elizabeth Grasty

USF Tampa Graduate Theses and Dissertations

Reef fish species tend to reside over high relief habitat which makes them difficult to sample with traditional gears such as nets and trawls. Therefore, implementing and understanding the strengths and weaknesses of new approaches which incorporate acoustic and optical methods has become a priority for reef fish stock assessment. Beginning in June of 2013, a towed camera system known as the Camera-Based Assessment Survey System (C-BASS) has been used to visualize over 500 kilometers of transect and record more than 80 hours of video over several habitats in the Gulf of Mexico. Surveys have been completed on the West …

Phenyl Functionalized Sol-Gel Silica Sorbent For Capillary Microextraction And Chromia-Based Sol-Gel Ucon Stationary Phase For Capillary Gas Chromatography, Michael M. Mclean

USF Tampa Graduate Theses and Dissertations

The first chapter of this thesis presents an introduction to sol-gel methodology whose usefulness as a synthetic route will be demonstrated with two applications in chromatography. The first application involves the fabrication of a capillary micro-extraction (CME) device by coating a phenyl functionalized extracting phase on the inner surface of a fused silica capillary for analyte pre-concentration. The device was coupled on-line to a RP-HPLC system and practicality was demonstrated using allergens as target analytes. The allergens chosen as model analytes are typically found in fragrance products and food. Most of the 26 fragrance allergens that are monitored by various …

Cloning And Characterization Of Il-1Β, Il-8, Il-10, And Tnfα From Golden Tilefish (Lopholatilus Chamaeleonticeps) And Red Snapper (Lutjanus Campechanus), Kristina L. Deak

USF Tampa Graduate Theses and Dissertations

Cytokines are pleiotropic and redundant signaling molecules that govern the inflammatory response and immunity, a critical ecological parameter for organism success and population growth. Produced at the site of injury or pathogen intrusion by a variety of cell types, cytokines mediate cell-signaling in either an autocrine or paracrine manner. The type and magnitude of the cytokine milieu produced subsequently dictates the strength and form of immune response. As the most diverse vertebrate group, with a high sensitivity to contaminants, fish represent an important foci for the evaluation of immune system evolution, function, and alteration upon toxicant exposure. While many cytokines …

Love And (M)Other (Im)Possibilities, Summer Renee Cunningham

USF Tampa Graduate Theses and Dissertations

This dissertation is a performative interrogation of the disagreement and (dis)interest, communication issues, surrounding motherhood in contemporary U.S. culture. Textual analysis of Mary Kelly's Post-Partum Document (PPD) plays a key role in my inquiry. I juxtapose documentation from my lived experiences and academic projects with Kelly's work to build upon the themes and ideas introduced throughout PPD. This project is guided by the concepts love and (im)possibility, and I will argue that, together, they are central to understanding mothering/caregiving as a site of communication inquiry. Love and (im)possibility are inherent to both mothering and communication, but they also are essential …

Quantification Of Protein Adhesion Strength To Surface Attached Poly (N- Isopropylacrylamide) Networks By Hydrodynamic Detachment Shear Stresses, Gulnur Sanden

USF Tampa Graduate Theses and Dissertations

Stimuli responsive coatings offer a versatile method by which to manipulate interfacial interactions of proteins in a desired way. However, there exists little guidance as to how the structure of a responsive polymer coating influences adsorption of proteins. In this dissertation, the adsorption behavior of immuglobulin G (IgG) on poly (N-isopropylacryamide) (PNIPAAm) hydrogel coatings was investigated as a function of film thickness. PNIPAAm exhibits a hydrophilic to hydrophobic transition above a critical temperature of ~32°C in aqueous solutions. In this research, through the use of quartz crystal microbalance with dissipation (QCM-D) it was observed that the adsorption was thickness dependent …

Penetration Through The Staple Punctures On Five N95 Respirator Models, Daniel E. Medina

USF Tampa Graduate Theses and Dissertations

Certain N95 FFR models that staple the head straps directly onto filtering material are commercially available. This method of assembly can tear or reduce fiber density in the immediate area surrounding the staple punctures. Five N95 FFR models were evaluated to determine if staple punctures on the filter medium reduce the protection offered by the respirators.

Total penetrations were measured with the staple punctures intact and when stretching the head straps a distance equivalent to a 95% male head circumference. Filter penetration were measured by sealing the staple punctures. Aerosols of 200, 500, and 1000 nm were used to challenge …

Blue Is In The Eye Of The Beholder: A Cross Cultural Study On Color Perception And Memory, Mark Douglas Lowry

USF Tampa Graduate Theses and Dissertations

According the linguistic relativity hypothesis, the language one speaks affects how one thinks. Because languages differ in how they categorize color, linguistic relativity has often been tested by conducting experiments on color perception and memory. This study examines the linguistic relativity hypothesis using ecologically valid stimuli: pictures of eyes. Because Russian-speakers are more likely to describe blue/grey eyes as grey, whereas English speakers are more likely to describe them as blue, English and Russian participants were asked to match the overall color of blue eyes to a color scale. There were three conditions. In the first condition (perception), participants saw …

Teaching With The End In Mind: A Teacher's Life History As A Legacy Of Educational Leaders, Daryl Adam Ward

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to understand the life history of a female teacher by examining her beliefs about leaving a teaching legacy and by analyzing the narratives of four educational leaders as they reflect on the generative behaviors of this teacher. The research questions guiding this study were: (a) What elements of this teacher's life history contributed to creating a legacy of educational leaders? (b) What are the perspectives of the educational leaders impacted by this teacher as they relate to crafting an educational legacy? (c) How do the life stories of the teacher and educational leaders intersect …

A Passive Membrane Photobioreactor For The Isolated Cultivation Of Algal Resource Utilizing Selectivity (Icarus), With Wastewater As A Feedstock, Ivy Lea Cormier Drexler

USF Tampa Graduate Theses and Dissertations

Renewed momentum in the microalgae industry due to commercial interest in biofuels and bioproducts is driving the need to increase the economic competitiveness of large-scale microalgal production. Current knowledge of membrane systems common in other disciplines, such as environmental engineering, marine science, and biomedicine, are relevant to algae production. With pore sizes ranging from microns to angstroms, membranes provide tailored functions for solid/liquid separation (cell retention, biomass concentration and dewatering), gas/liquid separation (gas delivery and removal), and solute/liquid separation (bioproduct recovery, feedstock preparation and effluent recycling) that are problematic or not possible with other technologies. Though membranes have great potential …

Medicaid Pays For That? An Exploratory, Mixed-Methods Analysis Of Florida Home Birth, Nicole K. Demetriou

USF Tampa Graduate Theses and Dissertations

The overwhelming social norm for pregnant women in the U.S. is to receive prenatal care from an obstetrician and to give birth in a hospital setting. However, the incidence of midwifery care and out-of-hospital birth is increasing, particularly among White, non-Hispanic women. Florida has been considered a "model" state for home birth midwifery given legislative support that mandates coverage of all types of midwifery (e.g., Certified Professional Midwives and Certified Nurse-Midwives) care in all birth settings (e.g., hospital, home, birth center) and by all forms of insurance (e.g., commercial and Medicaid). Medicaid is the payer source for nearly half of …

A Decision Support Model For Personalized Cancer Treatment, Florentino Antonio Rico-Fontalvo

USF Tampa Graduate Theses and Dissertations

This work is motivated by the need of providing patients with a decision support system that facilitates the selection of the most appropriate treatment strategy in cancer treatment. Treatment options are currently subject to predetermined clinical pathways and medical expertise, but generally, do not consider the individual patient characteristics or preferences. Although genomic patient data are available, this information is rarely used in the clinical setting for real-life patient care. In the area of personalized medicine, the advancement in the fundamental understanding of cancer biology and clinical oncology can promote the prevention, detection, and treatment of cancer diseases.

The objectives …

Wastewater Nutrient Recovery Using Anaerobic Membrane Bioreactor Permeate For Hydroponic Fertigation, Jorge Luis Calabria

USF Tampa Graduate Theses and Dissertations

The imbalance between global population growth and resource consumption is indicative of unsustainable practices and foreshadows a grim future of continued resource depletion, food and water scarcity, social inequality, and deteriorating public and environmental health. Meanwhile, the urban centers of the world continue to experience exponential growth resulting in overwhelmed food, water, and sanitation infrastructure. Decentralized and satellite wastewater treatment technologies capable of resource recovery, such as anaerobic membrane bioreactors (AnMBR), foster synergistic opportunities to help manage the food, energy, and water sectors of urban environments. Specifically, the nutrient concentration and high effluent quality of permeate produced by AnMBR systems …

The Effects Of Emotive Reasoning On Secondary School Students' Decision-Making In The Context Of Socioscientific Issues, Wardell Anthony Powell

USF Tampa Graduate Theses and Dissertations

The discrepancy between what students are being taught within K-12 science classrooms and what they experience in the real world has been well documented. This study sought to explore the ways a high school biology curriculum, which integrates socioscientific issues, impacts students' emotive reasoning and their ability to evaluate evidence, make informed decisions on contemporary scientific dilemmas, and integrate scientific content knowledge in their reasoning on SSI. Both quantitative and qualitative methods were used to examine differences within and between an SSI treatment group and a comparison group as well as individual differences among students' responses over a semester of …

Analysis Of Water Quality For Lake Tarpon, Pinellas County, Florida, Kristi Nichole Savio

USF Tampa Graduate Theses and Dissertations

Urbanization has taken a major toll on the water quality of Lake Tarpon. In response, there have been management strategies and legislation put in place to help improve water quality. Our objective was to identify what management strategies and other environmental factors were driving changes in Lake Tarpons water quality from 1970 - 2010. Trends in water quality were analyzed against precipitation, land use and water quality management strategies to achieve this. Results found that two water quality management strategies, the creation of the Lake Tarpon Outfall Canal and the closing of the Lake Tarpon Sink, improved water quality the …

Photonic Crystal-Based Flow Cytometry, Justin William Stewart

USF Tampa Graduate Theses and Dissertations

Photonic crystals serve as powerful building blocks for the development of lab-on-chip devices. Currently they are used for a wide range of miniaturized optical components such as extremely compact waveguides to refractive-index based optical sensors. Here we propose a new technique for analyzing and characterizing cells through the design of a micro-flow cytometer using photonic crystals. While lab scale flow cytometers have been critical to many developments in cellular biology they are not portable, difficult to use and relatively expensive. By making a miniature sensor capable of replicating the same functionality as the large scale units with photonic crystals, we …

Creating A Mythopoeic Graphic Novel To Expand Self-Understanding, Luis De La Lama

USF Tampa Graduate Theses and Dissertations

This is a study about how I produced a graphic novel to introduce a model of the self that is informed by complexity theory to an audience of comic book and graphic novel enthusiasts. Because this model of the self has the potential to preserve, extend, and/or reinforce character strengths that are operationalized as virtuous behavior, and that also function as inner resources in times of adversity, my study explores storytelling by sequential art as a communication method that some counselors and educators might use to counsel and educate large segments of popular culture. Also, more generally, I explore the …