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Droplet-Based Mechanical Actuator Utilizing Electrowetting Effect, Qi Ni

USF Tampa Graduate Theses and Dissertations

The goal of this work is to quantify the key design parameters such as the load capacity, actuation force, positioning repeatability, and reliability for droplet-based electrowetting actuators. Due to the fact that surface tension dominates gravity at both the mesoscale and microscale, droplet-based actuators can provide adequate force in manipulation tasks at those scales. Electrowetting, which uses an electric field to modulate the apparent surface tension of the liquid-ambient, provides a method to actuate droplets, which in turn transports the object carried by the droplet.

Most previous electrowetting actuation efforts have concentrated on manipulating droplets in a closed two-plate configuration. …

A Comparative Analysis Of Demographics And Reported Preferential Learning Modes Of Florida And Non-Florida Osher Lifelong Learning Institutes Members, Jung Min Lee

USF Tampa Graduate Theses and Dissertations

This research examined demographic factors and reported preferential learning mode among a sample of Florida Osher Lifelong Learning Institute (OLLI) members compared to a national sample profile of OLLI members. This study was prompted by an earlier study of OLLI members conducted by the National Resource Center (NRC) for OLLIs, which produced a national profile describing OLLI members. Although there was a national profile for OLLI members, there were no existing profiles of Florida OLLI members that could be used by OLLI administrators and instructors.

This study employed an online survey to compare data between the national OLLI member profile …

Targeting Tau Degradation By Small Molecule Inhibitors For Treatment Of Tauopathies, Mackenzie Martin

USF Tampa Graduate Theses and Dissertations

Tauopathies are neurodegenerative diseases that affect millions of people around the world. Tauopathies include more than 20 neurodegenerative diseases. Some of the most common tauopathies are Alzheimer’s disease (AD), frontotemporal dementia (FTD), chronic traumatic encephalopathy (CTE), Pick’s disease, corticobasal degeneration, progressive supranuclear palsy (PSP), agyrophillic grain disease, and amyotrophic lateral sclerosis (ALS). These diseases can cause significant memory loss, behavioral changes, motor deficits and speech impairments. Tauopathies stem from accumulation of the microtubule associated protein tau (MAPT). Tau stabilizes microtubules and helps with axonal transport. In a disease state tau becomes hyperphosphorylated and truncated leading to its aggregation. More recently …

Nano-Photonic Waveguides For Chemical And Biomedical Sensing, Surya Venkatasekhar Cheemalapati

USF Tampa Graduate Theses and Dissertations

In this dissertation, advances in the fields of Photonics, and Plasmonics, and specifically, single cell analysis and waveguide sensing will be addressed. The first part of the dissertation is on Finite Difference Time Domain (FDTD) optimization and experimental demonstration of a nano-scale instrument that allows sensing at the cellular and subcellular levels. A new design of plasmonic coupler into a nanoscale waveguide is proposed and optimized using FDTD simulations. Following this, a subcellular nanoendoscope that can locally excite fluorescence in labelled cell organelles and collect the emitted fluorescent light for detailed spectrum analysis is fabricated and tested. The nanoendoscope has …

Graphene Quantum Dots-Based Drug Delivery For Ovarian Cancer Therapy, Yiru Qin

USF Tampa Graduate Theses and Dissertations

Ovarian cancer, one of the most dreadful malignancies of the female reproductive system, poses a lethal threat to women worldwide. In this dissertation, the objective was to introduce a novel type of graphene quantum dots (GQDs) based nano-sized drug delivery systems (DDS) for ovarian cancer treatment. As a starting point, the facile synthesis method of the GQDs was established. Subsequently, the targeting ligand,folic acid (FA), was conjugated to GQDs. Next, a FDA approved chemotherapeutic drug, Doxorubicin (DOX), was loaded to form the GQDs-FA-DOX nano-conjugation as the DDS. Moreover, the uptake profile and anti-cancer effect of the GQDs-FA-DOX were validated in …

Understanding The Complexity Of Product Returns Management: A Complex Adaptive Systems Theory Perspective, Jennifer Anne Espinosa

USF Tampa Graduate Theses and Dissertations

The core essence of a marketing transaction is the exchange of value between two parties. Quite often, the exchange of value describes a customer purchasing a product from a company. When purchasing products, the exchange of value can often fail due to product defects or customer dissatisfaction. When the marketing exchange fails, customers often desire an avenue for recourse to right the exchange imbalance. Accepting and quickly processing product returns represents a strategic tool companies can leverage to maintain healthy relationships with customers, despite an exchange failure. Effectively managing product returns also benefits companies financially, by reducing inventory levels, costs, …

Neighborhood Perceptions Of Proximal Industries In Progress Village, Fl, Laura E. Baum

USF Tampa Graduate Theses and Dissertations

Progress Village, a historically Black neighborhood outside of Tampa, FL, encountered structural violence that included construction of an adjacent phosphogypsum stack. Why the neighborhood signed a legal agreement with the stack’s operating industry and the impacts of this decision provides a lesson in critical environmental justice. Theories of urban political ecology frame exploration of resident priorities, relationships with industry, risk perceptions, and health concerns. Utilizing activist anthropology, this thesis aims to be mutually beneficial to scholarly and neighborhood development. Ultimately, this research demonstrates how southern gradualism, racism, and a trend towards isolationism created today’s striving, yet marginalized and divided community. …

Growth, And Development Of Care For Leprosy Sufferers Provided By Religious Institutions From The First Century Ad To The Middle Ages, Philippa Juliet Meek

USF Tampa Graduate Theses and Dissertations

This thesis aims to outline the causes, symptoms, and treatments related to leprosy, and how it can be diagnosed in patients and identified in human remains. The thesis also aims to demonstrate the ways in which care for leprosy sufferers developed as the disease became more prevalent and more commonly, and correctly identified. It analyses the social stigmas inflicted upon sufferers, and the medical care and attention provided for them by religious institutions when other groups or organisations shunned those suffering from leprosy. The rationale for this study is to identify trends surrounding the social stigmas attached to leprosy and …

Gamma Aapeptides As Host Defense Peptide Mimics, Yaqiong Li

USF Tampa Graduate Theses and Dissertations

There has been increasing concern regarding the emergence of multi-drug resistant pathogens. The resistance develops when pathogens, especially bacteria, are frequently exposed to conventional antibiotics, as they are heavily used in both human and livestock. This is due to the high target specificity of conventional antibiotics, which places pathogens in high selective pressures and eventually results in drug resistant by mutations. To address this issue, global actions and cooperation are needed. At the same time, new technologies and strategies need to be developed. Host defense peptides (HDPs) are widely found in the innate immune system. They show both direct antimicrobial …

Beauty, Sex, And Death: The Role Of Mortality Salience In Objectification Processes, Kasey Lynn Morris

USF Tampa Graduate Theses and Dissertations

Although much attention has been paid to the consequences of objectification, relatively little research has focused on the question of why women are objectified. From a terror management theory perspective, the association of women with (literal) objects strips them of the qualities that are threatening (on account of mortality concerns). Sexualization, however, underscores women’s animal nature, and this association is problematic in the management of existential anxiety. The current research builds on a distinction between sexual and appearance-focused objectification to identify the existential mechanisms in the motivation to dehumanize, and subsequently harm, women. Consistent with the hypothesis, participants primed with …

An Exploration Of Adolescent Obesity Determinants, Anastasia King Smith

USF Tampa Graduate Theses and Dissertations

In 2010, approximately two-thirds of adults and one-fifth of the adolescent population in the United States were considered either overweight or obese, resulting in the United States having the highest per capita obesity rate among all OECD countries. A considerable body of literature regarding health behavior, health outcomes, and public policy exists on what the Centers for Disease Control and Prevention considers an obesity epidemic. In response to the growing problem of childhood obesity, the Child Nutrition and WIC Reauthorization Act of 2004 (CNRA), which required that schools participating in the National School Lunch Program and/or School Breakfast Program have …

Cell Permeability Studies Of Aapeptides And Novel Molecular Probes For Ad, Ge Bai

USF Tampa Graduate Theses and Dissertations

Alzheimer's diseases(AD) has been discovered and under research for more than 70 years, However there is no cure for these progressive and devastating diseases. Based on the following hypothsis: Aß metabolite problem/over production result in the accumulation, and lead to aggregation is the cause of Alzheimer’s disease. AApeptide and Melatonin derivatives can bind to Aß and block the aggregation of β amyloid monomers, decrease the toxicity of Aß to neurons and slow the progressive of Alzheimer’s diseases. In addition, AApeptide which mimic transmembrane peptide Tat will have similar transmembrane function. We have set up our goals as follows: 1) Using …

When Maps Ignore The Territory: An Examination Of Gendered Language In Cancer Patient Literature, Joanna Bartell

USF Tampa Graduate Theses and Dissertations

Cancer patients report having a high need for cancer information. Several studies show that the majority of patients surveyed report preferring information from the American Cancer Society (ACS). Ranging up to 129 pages, the ACS’ Detailed Guides (DG) are widely distributed throughout the United States, and offer patients an authoritative guide to help patients navigate the difficult terrain of the cancer journey. This dissertation examines the ACS’ cervical, endometrial, ovarian, penile, prostate, testicular, and vaginal cancer guides. Through a rhetorical analysis of the 7 guides, it was shown that the ACS DGs in question foster gendered narratives that strictly limit …

Stoicism In Descartes, Pascal, And Spinoza: Examining Neostoicism’S Influence In The Seventeenth Century, Daniel Collette

USF Tampa Graduate Theses and Dissertations

My dissertation focuses on the moral philosophy of Descartes, Pascal, and Spinoza in the context of the revival of Stoicism within the seventeenth century. There are many misinterpretations about early modern ethical theories due to a lack of proper awareness of Stoicism in the early modern period. My project rectifies this by highlighting understated Stoic themes in these early modern texts that offer new clarity to their morality. Although these three philosophers hold very different metaphysical commitments, each embraces a different aspect of Stoicism, letting it influence but not define his work. By addressing the Stoic themes on the morality …

Cardiovascular Effects Evoked By Airway Nociceptive Reflexes In Healthy And Cardiovascular Diseased Rats, Justin Shane Hooper

USF Tampa Graduate Theses and Dissertations

Acute inhalation of airborne pollutants alters cardiovascular function and has been shown to have its greatest affects on individuals with pre-existing cardiovascular disease. Evidence suggests that pollutant-induced activation of airway sensory nerves via the gating of ion channels is critical to these systemic responses. Here, we have investigated the cardiovascular responses evoked by inhalation of AITC (TRPA1 agonist) and capsaicin (TRPV1 agonist) in healthy Sprague Dawley (SD) and Wistar Kyoto (WKY) rats, and cardiovascular diseased Spontaneously Hypertensive (SH) rats. Inhalation of the agonists by healthy SD and WKY rats caused significant bradycardia, atrio-ventricular (AV) block and prolonged PR-Intervals. Inhalation of …

Chiral Boro-Phosphates In Asymmetric Catalysis: 1,4-Reduction Of Enones And Reductive Aldol, Susana Sorina Lopez

USF Tampa Graduate Theses and Dissertations

The biological activity of the pharmaceutical drugs often depends on how it fits with a receptor making stereochemistry a key component. Selective reactions can limit or avoid the mixture of enantiomers obtained. One such reaction is the selective reduction of a carbon-carbon double bond in the presence of a carbonyl. Although efficient, current asymmetric synthesis methods have limitations such as harsh reaction conditions, the high costs of chiral catalysts and the toxicity of the metal-based catalysts. Catalysts derived from small organic molecules have become an attractive alternative which have been explored more rigorously in recent years. Using a BINOL-derived boro-phosphate …

The Relationship Between Psychopathic Personality Traits And Lying, Jason A. Dobrow

USF Tampa Graduate Theses and Dissertations

The current study examined the relationship between psychopathic personality traits and various forms of deception. Through the use of the Elemental Psychopathy Assessment to measure psychopathy, and several different assessment tools to measure deception, including the Multidimensional Deception Inventory (MDI), the relationship between psychopathic personality traits and deception was examined. Using an undergraduate sample of 261 participants at a large research university in the Southeastern United States, the relationship between the aforementioned constructs was explored. Results indicated that the overarching personality traits of Antagonism and Disinhibition were positively related to multiple dimensions of lying behavior. Frequency of lies told, Duping …

Genetic Moderators Of Cognitive Decline In The Health And Retirement Study, Shannon K. Runge

USF Tampa Graduate Theses and Dissertations

The current dissertation used a gene x environment (G x E) approach to examine the independent and interactive effects of specific genetic variants and participation in physical and cognitive/social activities (PA and CSA) on cognitive performance in 4,764 participants of the Health and Retirement Study. Using three-wave data, three sets of multi-level growth models were conducted to examine baseline, longitudinal, and interactive effects of genotype (i.e., ApoE, COMT, and BDNF) and CSA/PA on performance across five cognitive measures: immediate, delayed and total word recall, and serial 7s and backwards counting.

At baseline, the ApoE ε4 allele predicted …

Mining Dynamic Recurrences In Nonlinear And Nonstationary Systems For Feature Extraction, Process Monitoring And Fault Diagnosis, Yun Chen

USF Tampa Graduate Theses and Dissertations

Real-time sensing brings the proliferation of big data that contains rich information of complex systems. It is well known that real-world systems show high levels of nonlinear and nonstationary behaviors in the presence of extraneous noise. This brings significant challenges for human experts to visually inspect the integrity and performance of complex systems from the collected data. My research goal is to develop innovative methodologies for modeling and optimizing complex systems, and create enabling technologies for real-world applications. Specifically, my research focuses on Mining Dynamic Recurrences in Nonlinear and Nonstationary Systems for Feature Extraction, Process Monitoring and Fault Diagnosis. …

“It’S Not Pixie Dust”: An Exploratory Qualitative Case Study Of A School-Based Multimodal Tablet Initiative, Erin Elizabeth Margarella

USF Tampa Graduate Theses and Dissertations

This dissertation presents findings from a qualitative case study of three English teachers representing varying levels of comfort with technology and years of teaching experience at St. Patrick Catholic High School. This research was motivated by two questions: (1) What are three literacy educators’ perceptions of a multimodal tablet initiative at a Catholic High School? (2) How is information regarding the use of iPad technology for literacy disseminated to three High School English teachers within a Catholic School system? Data was collected over a twelve-week period during the first academic year of implementation of a school-based multimodal tablet (iPad) initiative. …

Investigation Of Respiratory Syncytial Virus Structural Determinants And Exploitation Of The Host Ubiquitin System, Jillian Nicole Whelan

USF Tampa Graduate Theses and Dissertations

Respiratory syncytial virus (RSV) is a globally circulating, non-segmented, negative sense (NNS) RNA virus that causes severe lower respiratory infections. This study explored several avenues to ultimately expand upon our understanding of RSV pathogenesis at the protein level. Evaluation of RSV intrinsic protein disorder increased the relatively limited description of the RSV structure-function relationship. Global proteomics analysis provided direction for further hypothesis-driven investigation of host pathways altered by RSV infection, specifically the interaction between the RSV NS2 protein and the host ubiquitin system. NS2 primarily acts to antagonize the innate immune system by targeting STAT2 for proteasomal degradation. The goal …

Exploring The Relationship Between Severity Of Illness And Human Milk Volume In Very Low Birth Weight And Extremely Low Birth Weight Infants Over Six Weeks, Shannon Leigh Morse

USF Tampa Graduate Theses and Dissertations

Very low birth weight and extremely low birth weight neonates have tremendous risk of mortality. This is a grave concern; however, survival alone is not the goal of neonatal intensive care. Survival, along with a reduction or elimination of life long morbidity is the aim of neonatal intensive care.

Human milk is known as the best nutrition for babies and a growing body of evidence supports that human milk is critical in helping these fragile neonates mitigate the overwhelming risks they face. Therefore, the purpose of this study was to examine the relationship between neonatal severity of illness and human …

The Use Of Synthetic Mixture Based Libraries To Identify Hit Compounds For Eskape Pathogens, Leishmaniasis, And Inhibitors Of Palmitoylation, Marcello Giulianotti

USF Tampa Graduate Theses and Dissertations

The goal of this work is to demonstrate the utility of using systematically formatted mixture based libraries as part of the drug discovery processes. While there are a number of different valid approaches for identifying hit and tool compounds, systematically formatted mixture based libraries, such as those described in this study, offer the ability to develop a significant amount of structure activity relationship data from the testing of very few samples. In support of this claim a review of recent developments in the area of systematically formatted mixture based libraries as well as three case studies are presented. The three …

Co(Ii)-Based Metalloradical Catalysis For Asymmetric Radical Cyclopropanation, Qigan Cheng

USF Tampa Graduate Theses and Dissertations

Metal-catalyzed cyclopropanation of olefins with diazo reagents is one of the most general and efficient method to prepare cyclopropanes. The resulting cyclopropyl units are found as a basic structural element in a wide range of naturally occurring compounds and biologically active compounds, and can serve as versatile synthetic intermediates in the synthesis of multifunctionalized cycloalkanes and acyclic compounds. Since Co(II) complexes of D₂-symmetric chiral amidoporphyrins [Co(D₂-Por*)] were first introduced in 2004, Co(II)-metalloradical catalysis (MRC) have emerged as a new platform for asymmetric cyclopropanation. These metalloradical catalysts have been shown to be highly effective for asymmetric intermolecular …

Perceptions Of College Instructors Toward Accented English Measured By The Auditory Multifactor Implicit Association Test, Eunkyung Na

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to examine the implicit language attitudes of college-level instructors toward accented English and the effect of gender, teaching experience, and home language background on those attitudes. The auditory multifactor Implicit Association Test (IAT) was used to measure the implicit attitudes toward Standard, Chinese, Hispanic, and Korean accented English. For the current study, audio stimuli were embedded into the multifactor IAT, which became available for the first time in 2014. The auditory multifactor IAT generated implicit preference scores of six pairs of accented English: Standard vs. Chinese, Standard vs. Hispanic, Standard vs. Korean, Chinese vs. …

Nonreplicative Dna Helicases Involved In Maintaining Genome Stability, Salahuddin Syed

USF Tampa Graduate Theses and Dissertations

Double-strand breaks and stalled forks arise when the replication machinery encounters damage from exogenous sources like DNA damaging agents or ionizing radiation, and require specific DNA helicases to resolve these structures. Sgs1 of Saccharomyces cerevisiae is a member of the RecQ family of DNA helicases and has a role in DNA repair and recombination. The RecQ family includes human genes BLM, WRN, RECQL4, RECQL1, and RECQL5. Mutations in BLM, WRN, and RECQL4 result in genetic disorders characterized by developmental abnormalities and a predisposition to cancer. All RecQ helicases have common features including a …

Vegetative Responses To Hydrology And Ground Water Extraction In West-Central Florida Cypress Domes, Paul Thurman

USF Tampa Graduate Theses and Dissertations

The increasing demand for and limited supply of fresh water necessitates an understanding of how human actions affect aquatic ecosystems. Anthropogenic impacts to these ecosystems occur in many forms including eutrophication, invasive species removals, and hydrologic alterations. Ground water extraction is one such action that can dramatically impact wetland hydrology and is increasing in occurrence globally as clean surface water resources are exhausted. Despite the importance of ground water extraction to meet human demand, little information is available concerning the response of vegetation communities to chronic ground water extraction. Over extraction is known to result in reduced water levels and …

Costumbres, Creencias, Y “Lo Normal”: A Biocultural Study On Changing Prenatal Dietary Practices In A Rural Tourism Community In Costa Rica, Allison Rachel Cantor

USF Tampa Graduate Theses and Dissertations

This study explores the relationship between tourism, the nutrition transition, and prenatal dietary practices in the Monteverde Zone, Costa Rica. This rural tourism community, located in the central highlands of Costa Rica, has seen rapid growth and development since the tourism boom in the early 1990s, leading to changes in the local food system and increased food insecurity. This investigation added to this work by identifying the ways that prenatal dietary practices have shifted over time in the context of increased tourism and the concomitant nutrition transition, and by describing the relationship between food insecurity and nutritional status among pregnant …

Targeting Histone Deacetylases In Melanoma And T-Cells To Improve Cancer Immunotherapy, Andressa Sodre De Castro Laino

USF Tampa Graduate Theses and Dissertations

Histone deacetylases (HDACs) are key mediators of gene expression and, thus, major regulators of cell function. As such, HDACs play a role in orchestrating tumor biology, and the use of small inhibitors targeting theses proteins is attractive for the field of cancer therapy. Indeed, several HDAC inhibitors have received FDA-approval for the treatment of malignancies, while a myriad of these compounds continue to be evaluated in clinical trials. Besides their direct impact on tumor growth, HDAC inhibitors have been shown to increase immunogenicity of cancer cells, facilitating generation of a productive immune response against tumors. Immunotherapeutic approaches take advantage of …

Structure-Interaction Effects In Novel Nanostructured Materials, Nam B. Le

USF Tampa Graduate Theses and Dissertations

Recent advances in experimental and computational methods have opened up new directions in graphene fundamental studies. In addition to understanding the basic properties of this material and its quasi-one dimensional structures, significant efforts are devoted to describing their long ranged dispersive interactions. Other two-dimensional materials, such as silicene, germanene, and transition metal dichalcogenides, are also being investigated aiming at finding complementary to graphene systems with other "wonder" properties. The focus of this work is to utilize first principles simulations methods to build our basic knowledge of structure-interaction relations in two-dimensional materials and design their properties. In particular, mechanical folding and …