Digital Commons Network^™

Unraveling The Wild: A Cultural Logic Of Animal Stories In Contemporary Social Life, Damien Contessa

USF Tampa Graduate Theses and Dissertations

This dissertation is about the stories people tell about animals when they don’t do what they are expected to do in contemporary social life. More specifically, it examines three case studies where “wild” animals unexpectedly challenge, transgress, or blur socially defined boundaries in public spaces. Drawing on cultural and interactionist studies of animals and environment, I explore popular animal stories written in news media, social media, and enacted in situ. Each qualitative case study illustrates a moment in time/space where the surprising movements or presence of wild animals causes the cultural categories of wildness/order to breakdown and destabilize. These “surface …

Increasing 18f-Fdg Pet/Ct Capabilities In Radiotherapy For Lung And Esophageal Cancer Via Image Feature Analysis, Jasmine Alexandria Oliver

USF Tampa Graduate Theses and Dissertations

Positron Emission Tomography (PET) is an imaging modality that has become increasingly beneficial in Radiotherapy by improving treatment planning (1). PET reveals tumor volumes that are not well visualized on computed tomography CT or MRI, recognizes metastatic disease, and assesses radiotherapy treatment (1). It also reveals areas of the tumor that are more radiosensitive allowing for dose painting - a non-homogenous dose treatment across the tumor (1). However, PET is not without limitations. The quantitative unit of PET images, the Standardized Uptake Value (SUV), is affected by many factors such as reconstruction algorithm, patient weight, and tracer uptake time (2). …

Biophysical Investigation Of Amyloid Formation And Their Prion-Like Self-Replication, Mentor Mulaj

USF Tampa Graduate Theses and Dissertations

Growth and deposition of amyloid fibrils, polymers of proteins with a cross beta-sheet structure, are associated with a significant number of human pathologies including Alzheimer’s disease, Parkinson’s disease, prion diseases, type II diabetes, and senile systematic or dialysis-related amyloidoses. The broader objective of my research is to identify the basic mechanisms regulating nucleation and growth of amyloid fibrils. There is increasing evidence that amyloid formation may proceed along at least two distinct assembly pathways for the formation of rigid fibrils. One pathway involves the nucleated polymerization of the characteristic rigid fibrils from partially denatured monomers and the other proceeds via …

Novel Techniques In Chemical Ecology To Examine Life Histories In Fishes, Orian Tzadik

USF Tampa Graduate Theses and Dissertations

Diet and movements in fishes are often logistically challenging to study. Trace element and stable isotope analyses have advanced these fields considerably, but are still constrained by methodological impediments, such as the tendency towards lethal sampling. Studying endangered fishes is particularly challenging as representative samples are difficult to obtain. However, the information gained from such studies is often critical to the recovery of endangered fishes as knowledge of life history attributes has the potential to greatly influence the success of management strategies.

I tested the viability of using fin rays in fishes as a non-lethal approach to study diet and …

Optimization And Characterization Of Integrated Microfluidic Surface Acoustic Wave Sensors And Transducers, Tao Wang

USF Tampa Graduate Theses and Dissertations

Surface acoustic waves (SAWs) have a large number of applications and the majority of them are in the sensor and actuator fields targeted to satisfy market needs. Recently, researchers have focused on optimizing and improving device functions, sensitivity, power consumption, etc. However, SAW actuators and sensors still cannot replace their conventional counterparts in some mechanical and biomedical areas, such as actuators for liquid pumping under microfluidic channels and sensors for real-time cell culture monitoring. The two objectives of this dissertation are to explore the potential of piezoelectric materials and surface acoustic waves for research on actuators and sensors in the …

Acropora Habitat Evaluation And Restoration Site Selection Using A Species Distribution Modeling Approach, Katherine Wirt Ames

USF Tampa Graduate Theses and Dissertations

While populations of nearly all stony coral species along the Florida reef tract have exhibited decline, the most notable decline has occurred in the once-dominant acroporid species (Acropora cervicornis, A. palmata). Both species were listed in 2006 as threatened under the Endangered Species Act. This listing, combined with their continued decline, has resulted in large-scale restoration efforts throughout Florida and the Western Caribbean. Currently, there is little to no information regarding spatial prioritization of sites for these restoration efforts. The primary objective of this dissertation was to utilize species distribution modeling, informed by existing data from the …

Modeling And Survival Analysis Of Breast Cancer: A Statistical, Artificial Neural Network, And Decision Tree Approach, Venkateswara Rao Mudunuru

USF Tampa Graduate Theses and Dissertations

Survival analysis today is widely implemented in the fields of medical and biological sciences, social sciences, econometrics, and engineering. The basic principle behind the survival analysis implies to a statistical approach designed to take into account the amount of time utilized for a study period, or the study of time between entry into observation and a subsequent event. The event of interest pertains to death and the analysis consists of following the subject until death. Events or outcomes are defined by a transition from one discrete state to another at an instantaneous moment in time. In the recent years, research …

Disinhibition, Violence Exposure, And Delinquency: A Test Of How Self-Control Affects The Impact Of Exposure To Violence, Wyatt Brown

USF Tampa Graduate Theses and Dissertations

This dissertation examines the role of self-control in the relationship between exposure to violence and antisocial behavior. Specifically, this study proposes that the impact of exposure to violence changes depending on internalized factors such as self-control. Individuals with high exposure to violence but greater levels of self-control may be less influenced by the impact of exposure to violence. Conversely, individuals with low levels of exposure to violence and lower levels of self-control may be more influenced by the impact of violence. The findings from this study suggest that there is some buffering effect on the impact of exposure to violence …

"The Afro That Ate Kentucky": Appalachian Racial Formation, Lived Experience, And Intersectional Feminist Interventions, Sandra Louise Carpenter

USF Tampa Graduate Theses and Dissertations

This thesis examines selections of Appalachian women’s personal narrative as well as Affrilachian Poetry written by Kentuckians Bianca Spriggs and Nikki Finney. This project’s goal lies in resisting oppression and erasure of Appalachian culture’s heterogeneity. Contrary to constructions of Appalachians as lazy, complacent, and white, many Appalachians organize communities of resistance from within the region itself. Challenging these representations, I argue that Appalachian feminists as well as Affrilachian poets create countercultures that disrupt monolithic, colonialist, and unquestioned constructions of Appalachia.

Sizing An Anaerobic Digester In A Rural Developing World Community: Does Household Fuel Demand Match Greenhouse Gas Production?, Ronald Keelan Greenwade

USF Tampa Graduate Theses and Dissertations

Anaerobic digestion is the process by which organic carbon is converted into biogas in the form of carbon dioxide (CO₂) and methane (CH₄). Both of these products are greenhouse gases that contribute to global warming. Therefore if anaerobic reactors are improperly maintained and biogas is leaked or intentionally released into the atmosphere because biogas production exceeds household demand, these reactors may become generators of greenhouse gas emissions instead of sustainable energy producers. The objective of this research was to develop a framework to assess if the demand for biogas by a rural adopter of an anaerobic …

Optimizing Protocols For Carbohydrate Nmr Chemical Shift Computations, Michael Trent Kemp

USF Tampa Graduate Theses and Dissertations

The spectroscopic analysis of cellulose is experimentally challenging while computationally accessible with recent developments in NMR code. However, prior to using density functional theory to calculate the NMR chemical shifts of cellulose, smaller, sugar-like molecule systems need to be benchmarked against experimental values. The quantum mechanical / molecular mechanical (QM/MM) calculations presented herein utilize six test systems: ethanol, pyridine, pyrrolidine, pyrrole, myo-inositol and scyllo-inositol in conjunction with the reference tetramethylsilane used to scale the calculated isotropic shielding tensors to relative chemical shifts. The effect of solvent on calculated NMR chemical shifts has also been investigated with regard to quantity of …

Modulation Of Whole Cell Currents In Human Neuroblastoma Cells Via The Hormone Aldosterone: An In Vitro Study, Harish Kumar Chittam

USF Tampa Graduate Theses and Dissertations

Ion channels play a critical role in maintaining homeostasis by moving various ions in and out of cells. The Na⁺-K⁺-2Cl^- or NKCC1 ion channel is involved in the regulation of Na⁺, K⁺, and Cl^- across cell membranes, and plays a key role in many forms of cellular physiology. In the cochlea, NKCC1 is involved in endolymph production and maintenance of the endocochlear potential. Our hypothesis is that blocking NKCC1 channels should directly impact auditory sensitivity causing hearing loss. Our lab has also shown that the hormone aldosterone (ALD) can upregulate …

Resilience In Uncertainty: An Examination Of A Moroccan Centre Serving Unwed Mothers, Dana Louise Goodlett

USF Tampa Graduate Theses and Dissertations

Utilizing a gender-based violence approach, this study investigated service delivery realities for a Moroccan women’s centre serving unwed mothers and their babies. Primary research methods included participant observation and semi-structured interviews (n=20) with unwed mothers and centre staff. This study aimed to determine what factors lead mothers to seek assistance, types of assistance offered, and challenges and future opportunities for services. Findings indicate a lack of social support to mothers, lack of social and economic support for the centre and reduced service capacity, and the use of deceit in interactions between mothers and staff rooted in cultural notions of shame. …

Conversion Of Landfill Gas To Liquid Hydrocarbon Fuels: Design And Feasibility Study, Ryan Alexander Kent

USF Tampa Graduate Theses and Dissertations

This paper will discuss the conversion of gas produced from biomass into liquid fuel through the combination of naturally occurring processes, which occur in landfills and anaerobic digesters, and a gas-to-liquids (GTL) facility. Landfills and anaerobic digesters produce gases (LFG) that can be converted into syngas via a Tri-reforming process and then synthesized into man-made hydrocarbon mixtures using Fischer-Tropsch synthesis. Further processing allows for the separation into liquid hydrocarbon fuels such as diesel and gasoline, as well as other middle distillate fuels. Conversion of landfill gas into liquid fuels increases their energy density, ease of storage, and open market potential …

Critical Heat Stress Evaluation In Two Ebola Ensembles, Christopher T. Lee

USF Tampa Graduate Theses and Dissertations

Ebola, a type of filovirus that causes hemorrhagic fevers, dominated global headlines in 2014 when the largest Ebola epidemic in history took place in West Africa. Healthcare practitioners were at particular risk of contracting Ebola while taking care of patients with the disease because they were easily exposed to bodily fluids such as blood, urine, saliva, and feces, quite often in the intensive care unit (ICU). While personal protective equipment (PPE) protects the healthcare practitioner by providing an effective barrier against the virus, users were also at risk for heat stress because of the type of protective clothing. In this …

Assessment Of Acrolein-Induced Toxicity Using In-Vitro Modeling To Evaluate The Role Of Parp Inhibitors In Reducing Cytotoxicity, Kristina Marie Harand

USF Tampa Graduate Theses and Dissertations

Acrolein is an electrophilic α, β-unsaturated aldehyde. Additionally, acrolein is a metabolite of the antineoplastic alkylating agent cyclophosphamide and is implicated in off-target effects, including to bladder hemorrhagic cystitis and cyclophosphamide-induced cardiotoxicity, both of which have led to serious secondary iatrogenic injury during and following chemotherapy. At low concentrations acrolein inhibits cell proliferation without inducing apoptosis, while at high concentrations may result in secondary apoptosis promotion. This investigation assessed the role of the enzyme poly (ADP-ribose) polymerase (PARP) in acrolein induced toxicity using the established toxicological H9c2 (2-1) cardiomyoblast in vitro model. H9c2 (2-1) cells were plated in 24-well plates …

“Even Five Years Ago This Would Have Been Impossible:” Health Care Providers’ Perspectives On Trans* Health Care, Richard S. Henry

USF Tampa Graduate Theses and Dissertations

Trans* studies and issues have recently increased in coverage by the media and popular press. With recent changes in the DSM-5 (APA, 2000; APA 2013) and insurance law (HHS, 2014), trans* healthcare has been under increasing scrutiny. While a small number of studies (Bradford, Reisener, Honnold, & Xavier, 2013; Grant et al., 2011; Rounds, McGrath, & Walsh, 2013; Tanner et al., 2014) have documented discrimination and lack of cultural competencies from the perspective of trans* patients, little research exists that examines the training, support, and decision-making processes of medical professionals who treat trans* patients (Snelgrove et al., 2012, p. 2). …

A Trans-Dimensional View Of Drug Resistance Evolution In Multiple Myeloma Patients, Timothy Jacobson

USF Tampa Graduate Theses and Dissertations

Multiple Myeloma (MM) is a treatable, yet incurable, malignancy of bone marrowplasma cells. This cancer affects many patients and many succumb to relapse of tumor burden despite a large number of available chemotherapeutic agents developed for therapy. This is because MM tumors are heterogeneous and receive protection from therapeutic agents by the microenvironment and other mechanisms including homologous MM-MM aggregation. Therefore, therapy failure and frequent patient relapse is due to the evolution of drug resistance, not a lack of available drugs. To analyze and understand this problem, the evolution of drug resistance has been explored and presented herein. We seek …

Experimental Studies Of Simultaneous Nitrification Denitrification And Phosphorus Removal At Falkenburg Advanced Wastewater Treatment Plant, Ann Elizabeth Sager

USF Tampa Graduate Theses and Dissertations

The discharge of point- and non-point source pollutants into surface waters resulting from industrial and/or municipal activities is a major focus of environmental regulation in the United States. As a result, the National Pollutant Discharge Elimination System (NPDES) permit program was established in 1972 in an effort to regulate discharges from industrial or municipal sources, including wastewater treatment plants (WWTP). To further protect Florida water quality, in 1978, State legislation enacted the Grizzle-Figg Act for Tampa Bay, which requires advanced wastewater treatment for any discharge into sensitive water bodies. A common use of wastewater effluent in the Tampa Bay area …

"But Our Hands Are Tied": Assessing School Gardening Efforts At Title I Elementary Schools In Pinellas County, Florida, Alexandra Grace Lancey

USF Tampa Graduate Theses and Dissertations

This research was designed to understand current school gardening efforts in Pinellas County, Florida. School gardens have become an important aspect of experiential learning and nutrition education in schools throughout the United States. Many not-for-profit organizations have attempted to increase the prevalence and efficacy of school garden programs as a means of providing educational opportunities and working to curb diet-related health issues in children. Most of these organizations are seen as apolitical in nature, because they access mainly private sector funding sources and volunteer support. This provides flexibility for these social projects, but also takes pressure off of the state …

The Relationship Between Learning Styles And The Choice Of Learning Environment For Hospitality And Tourism Undergraduate Students, Gunce Malan-Rush

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to explore the relationship between learning styles and the choice of learning environment for Hospitality and Tourism undergraduate students. An anonymous two-part survey was sent to the instructors of Introduction to Hospitality and Tourism Management courses (both online and face-to-face) in four schools in the state of Florida. The survey was designed to gather information related to the following three research questions related to MBTI profiles for undergraduate students in attempt to identify differences between students enrolled in online classes and those in face-to-face classes. In order to determine the probability of predicting course …

The Effect Of Physical Activity On Youths’ Cognitive, Academic, And Behavioral Outcomes: A Meta-Analysis Of Single Case Design Studies, Brett Allyn Stone

USF Tampa Graduate Theses and Dissertations

A third of youth in the United States are currently classified as overweight, which is impacted by the low rates of youth that meet daily physical activity recommendations. Engaging in physical activity contributes to healthy body weight and physical fitness, both of which have positive health consequences. Participating in physical activity not only aids in positive health outcomes, but research indicates that it also has a positive relationship with and effect on youths’ cognitive, academic, and behavioral outcomes. The majority of researchers conducting meta-analyses examining the effect of physical activity on youths’ cognitive, academic, and behavioral outcomes have excluded single-case …

West Florida Shelf Connectivity: An Exploratory Study, Amanda Sue Reinert

USF Tampa Graduate Theses and Dissertations

This Thesis explores the connectiveness of the West Florida Shelf’s various areas of economic and ecological importance by considering five case studies of varying dynamic forcing influences and time. The advection of water about the shelf moves nutrients and has a direct impact on the shelf’s ecology and the determination of whether or not the shelf will be oligotrophic at any given time or location. The case studies are analyzed both quantitatively and qualitatively after quasi-isopycnal particle trajectory calculations are completed for each. The findings support a combination of local and deep-ocean forcing being ideal for the maximum advection and …

An Examination Of The Distribution Of Diabetes Mellitus Among Tb Patients With Pulmonary Tuberculosis And Drug Resistant Tuberculosis In The State Of Florida, Usa., Mandzisi Mbongeni Mkhontfo

USF Tampa Graduate Theses and Dissertations

Background: Pulmonary Tuberculosis (PTB) is considered a disease of the past but it remains a major cause of mortality among immune compromised patients and continues to be a significant threat to public health globally. Notably, the prevalence of diabetes mellitus (DM) has increased over the years. The biological link of TB and DM has been reported in numerous literature with DM attributed to three folds increase in the risk of TB and linked to Drug Resistant TB, especially amongst aged diabetic patients. The aim of the study was to examine the distribution of DM among TB patients and explore …

In Vitro And In Vivo Antioxidant Capacity Of Synthetic And Natural Polyphenolic Compounds Identified From Strawberry And Fruit Juices, Marvin Abountiolas

USF Tampa Graduate Theses and Dissertations

Strawberries can be considered a functional food because their consumption has been associated with several health benefits. They are important sources of bioactive compounds, such as vitamins and polyphenolic compounds, with recognized antioxidant capacity (AOC). However, strawberry overall quality and bioactive content are greatly affected by environmental conditions during pre- and post-harvest and, little is known about the stability of its bioactive compounds, specifically ascorbic acid (AA) and polyphenolics compounds. Furthermore, additional research that addresses the impact of polyphenolic compounds on in vitro and in vivo models is needed to understand the mechanisms behind their potential health benefits. Therefore, the …

Multiple Case Study Of Factors Associated With Enrollment Of Women With Persistent Or Recurrent Ovarian Cancer In Clinical Trials In Central Florida, Judith Ann Ebbert

USF Tampa Graduate Theses and Dissertations

Study Purpose: Ovarian cancer, the most lethal gynecologic cancer, has had a relatively stable mortality rate since 1975, despite a decrease in mortality for all gynecologic cancers combined. Standard-of-care advances are needed to reduce ovarian cancer morbidity and mortality. Advances must, however, undergo a long, rigorously controlled research process that can take more than ten years before becoming available to the public. Further, few women with persistent or recurrent ovarian cancer are offered or enrolled in the clinical trials that do exist at select sites throughout the nation. The purpose of this multiple-case study is to identify necessary and/or sufficient …

Characterization Of Bacterial Diversity In Cold-Water Anthothelidae Corals, Stephanie Nichole Lawler

USF Tampa Graduate Theses and Dissertations

Cold-water corals, similar to tropical corals, contain a diverse and complex microbial landscape. Comprised of vital microscopic organisms (i.e. bacteria, viruses, archaea), the coral microbiome is a driving factor in the proliferation and survival of the coral host. Bacteria provide essential biological functions within coral holobionts, facilitating increased nutrient utilization and production of antimicrobial compounds. To date, few cold-water octocoral species have been analyzed to explore the diversity and abundance of their microbial associates. For this study, 23 samples of the family Anthothelidae were collected from Norfolk (n = 12) and Baltimore Canyons (n = 11) from the western Atlantic …

Regulatory Effect Of Elastin Based Biomaterial On Cellular Behavior And Its Application On Wound Repair And Regeneration, Yuan Yuan

USF Tampa Graduate Theses and Dissertations

Elastin-like peptides (ELPs) are stimulus-responsive protein-based polymers which are attractive material for biomedical research due to their biocompatibility and unique properties. The physical properties of ELPs are dependent on the chain length and the chosen amino acid at the guest residue position. This imparts unlimited flexibility in designing ELP based biomaterials with the desired physical properties.

We have shown that in addition to their physical properties, ELPs have biological activities that are conducive to tissue regeneration. Specifically, we found that ELPs induce fibroblast proliferation via cell surface heparan sulfate proteoglycans (HSPG). Furthermore, our data suggests that ELP based materials with …

Exciton Dynamics And Many Body Interactions In Layered Semiconducting Materials Revealed With Non-Linear Coherent Spectroscopy, Prasenjit Dey

USF Tampa Graduate Theses and Dissertations

Atomically thin, semiconducting transition metal dichalogenides (TMDs), a special class of layered semiconductors, that can be shaped as a perfect two dimensional material, have garnered a lot of attention owing to their fascinating electronic properties which are achievable at the extreme nanoscale. In contrast to graphene, the most celebrated two-dimensional (2D) material thus far; TMDs exhibit a direct band gap in the monolayer regime. The presence of a non-zero bandgap along with the broken inversion symmetry in the monolayer limit brands semiconducting TMDs as the perfect candidate for future optoelectronic and valleytronics-based device application. These remarkable discoveries demand exploration of …

Investigating Alternative Subsistence Strategies Among The Homeless Near Tampa, Florida, Matthew Peter Rooney

USF Tampa Graduate Theses and Dissertations

Modern homelessness is one of the most pressing social and political problems of our time. Several hundred thousand people experience homelessness in the United States each year, and the U.S. Department of Housing, which attempts to count those people, has admitted that their statistics are conservative estimates at best. A recent archaeological study (Zimmerman et al 2010) examining material culture associated with homeless communities in Indianapolis has suggested that those who are considered chronically homeless have generally abandoned wage labor and are instead pursuing urban foraging as a subsistence strategy. In order to better understand the structures of homeless communities, …