Digital Commons Network^™

Blue-Collar Scholars: Bridging Academic And Working-Class Worlds, Nathan Lee Hodges

USF Tampa Graduate Theses and Dissertations

This dissertation explores one white working-class family’s hopes, fears, illusions, and tensions related to social mobility. I tell stories from my experiences as a first-generation college student, including: ethnographic fieldwork; interviews with my family, community members, and former teachers; and narratives from other working-class academics to provide an in-depth, evocative, and relational look at mobility. I explore the roots of vulnerability in my family and how I was socialized into understanding belonging and worthiness in particular ways, and how this socialization influences my feelings of belonging and worthiness in the academy. The goal of this research is bridging – past …

The Role Of Prep Schools In The Middle To High School Transition Of Students In Southeastern Turkey, Mucahit Kocak

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to investigate the role that a small sample of prep-schools play in meeting the academic and behavioral needs of students who are at risk for poor academic and social outcomes as they transition from middle school to high school. The study examined the perspectives of teachers, students and families about their experiences with prep schools. A qualitative case research design was used in this research to gain a deeper understanding about the role of prep schools in the Southeastern region of Turkey. Teachers, students, mentors and parents were selected purposefully for the study as …

A Life Cycle Assessment Of A Uranium Mine In Namibia, Janine Lambert

USF Tampa Graduate Theses and Dissertations

Uranium mining and nuclear power is a controversial topic as of late, especially in light of the recent Fukushima event. Although the actual use of nuclear fuel has minimal environmental impact, its issues come at the very beginning and end of the fuel’s life cycle in both the mining and fuel disposal process. This paper focuses on a life cycle analysis (LCA) of uranium mine in the desert nation of Namibia in Southern Africa. The goal of this LCA is to evaluate the environmental effects of uranium mining. The LCA focuses on water and energy embodiment such that they can …

The Psychometric Evaluation And Validation Of A Measure Assessing Pharmacological And Social Alcohol Expectancies In Adolescents, Megan Victoria Mcmurray

USF Tampa Graduate Theses and Dissertations

Extending prior alcohol expectancy measurement research, this researcher (McMurray, 2013) recently developed the Pharmacological and Social Alcohol Expectancy Scale (PSAES). The PSAES is the only alcohol expectancy measure to date that provides adequate coverage of both social expectancies and the anticipated positive pharmacological effects resulting from alcohol consumption, and was developed and validated in a sample of young adults (aged 18-23). Research has shown that adolescents at high risk for alcohol use disorder (AUD) hold higher expectations of reward from alcohol, suggesting that expectancy patterns may help distinguish at-risk youth. Building upon the previous PSAES validation study, the primary purpose …

Rule-Based Risk Monitoring Systems For Complex Datasets, Mona Haghighi

USF Tampa Graduate Theses and Dissertations

In this dissertation we present rule-based machine learning methods for solving problems with high-dimensional or complex datasets. We are applying decision tree methods on blood-based biomarkers and neuropsychological tests to predict Alzheimer’s disease in its early stages. We are also using tree-based methods to identify disparity in dementia related biomarkers among three female ethnic groups. In another part of this research, we tried to use rule-based methods to identify homogeneous subgroups of subjects who share the same risk patterns out of a heterogeneous population. Finally, we applied a network-based method to reduce the dimensionality of a clinical dataset, while capturing …

Diversity And Distribution Of Diatom Endosymbionts In Amphistegina Spp. (Foraminifera) Based On Molecular And Morphological Techniques, Kwasi H. Barnes

USF Tampa Graduate Theses and Dissertations

Diatoms associated with foraminifers of the genus Amphistegina were assessed using a combination of morphological and molecular techniques. These included: 1) microscopic identification of diatoms cultured from the host, 2) sequencing of portions of the small subunit of the ribosomal RNA gene (18S) and the large subunit of the ribulose-1,5-bisphosphate carboxylase/oxygenase [i.e., RubisCO] gene (rbcL) from DNA extracted directly from the Amphistegina hosts and also from diatoms cultured from these hosts, and 3) denaturing gradient gel electrophoresis (DGGE) profiles of rbcL and internal transcribed spacer 1 (ITS1) PCR amplicons from DNA extracted directly from …

From Tolerance To Transmission: Linking Within-Individual To Community-Level Disease Processes, Sarah Catherine Burgan

USF Tampa Graduate Theses and Dissertations

Hosts have two main strategies for coping with infections: resistance and tolerance. Resistance is aimed at preventing or eliminating parasites, whereas the goal of tolerance is to maintain performance regardless of parasite burden. The balance between resistance and tolerance within a host may mediate host competence, or the propensity of a host to infect other hosts or vectors. Hosts with high tolerance and low resistance to an infection, for instance, may be highly competent and possess the greatest potential to act as superspreaders. These superspreading hosts will contribute disproportionately to transmission, thus posing the greatest risk to other hosts within …

Tunable Nano-Delivery System For Cancer Treatment: A New Approach For Targeted Localized Drug Delivery, Rana Falahat

USF Tampa Graduate Theses and Dissertations

Localized drug delivery systems have been widely studied as potential replacements for conventional chemotherapy with the capability of providing sustained and controlled drug release in specific targeted sites. They offer numerous benefits over conventional chemotherapy such as enhancing the stability of embedded drugs and preserving their anticancer activity, providing sustained and controlled drug release in the tumor site, reducing toxicity and diminishing subsequent side effects, minimizing the drug loss, averting the need for frequent administrations, and minimizing the cost of therapy.

The aim of this study is to develop a localized drug delivery system with niosomes embedded in a chitosan …

Smoking Among Youth Living With Hiv: The Intersection Of Behavioral Health And Chronic Disease, Todd Wells

USF Tampa Graduate Theses and Dissertations

Smoking amongst youth living with HIV (YLWH) has major public health implications. When combined with the complications associated with smoking such as chronic obstructive pulmonary disorder (COPD) or heart disease, the impact of a HIV positive diagnosis increases morbidity exponentially (Nahvi & Cooperman, 2009). The purpose of this study was two-fold, to investigate the perceptions of smoking amongst YLWH aged 18-25 who received care at the CMS Clinic located at the University of South Florida and to determine factors related to cessation. A study design utilizing two components, a systematic literature review and primary qualitative data was used in this …

A Feminist Contestation Of Ableist Assumptions: Implications For Biomedical Ethics, Disability Theory, And Phenomenology, Christine Marie Wieseler

USF Tampa Graduate Theses and Dissertations

This dissertation contributes to the development of philosophy of disability by drawing on disability studies, feminist philosophy, phenomenology, and philosophy of biology in order to contest epistemic and ontological assumptions about disability within biomedical ethics as well as within philosophical work on the body, demonstrating how philosophical inquiry is radically transformed when experiences of disability are taken seriously.

In the first two chapters, I focus on epistemological and ontological concerns surrounding disability within biomedical ethics. Although disabled people and their advocates have been quite vocal regarding their views on disability and in critiquing bioethicists’ approaches to issues that affect them, …

Impact Of Sludge Layer Geometry On The Hydraulic Performance Of A Waste Stabilization Pond, Faissal Romaric Ouedraogo

USF Tampa Graduate Theses and Dissertations

Improving the hydraulic performance of waste stabilization ponds (WSPs) is an important management strategy to not only ensure protection of public health and the environment, but also to maximize the potential reuse of valuable resources found in the treated effluent. To reuse effluent from WSPs, a better understanding of the factors that impact the hydraulic performance of the system is needed. One major factor determining the hydraulic performance of a WSP is sludge accumulation, which alters the volume of the pond.

In this study, computational fluid dynamics (CFD) analysis was applied to investigate the impact of sludge layer geometry on …

Biophysical Characterization And Theoretical Analysis Of Molecular Mechanisms Underlying Cell Interactions With Poly(N-Isopropylacrylamide) Hydrogels, Michael C. Cross

USF Tampa Graduate Theses and Dissertations

So-called, “Dynamic biomaterials” comprised of stimuli-responsive hydrogels are useful in a wide variety of biomedical applications including tissue engineering, drug delivery, and biomedical implants. More than 150,000 peer-reviewed articles (as of 2016) have been published on these materials, and more specifically, over 100,000 of these are on the most widely studied, poly(N-isopropylacrylamide). This thermoresponsive polymer in a crosslinked hydrogel network undergoes a large volume phase transition (𝑉/𝑉₀ ~ 10 − 100) within a small temperature range (𝑇 ~ 1 − 3𝐾) making it particularly useful for tissue engineering applications because of the ability to control the topographical configuration of …

Intrinsic Disorder Where You Least Expect It: The Incidence And Functional Relevance Of Intrinsic Disorder In Enzymes And The Protein Data Bank, Shelly Deforte

USF Tampa Graduate Theses and Dissertations

Intrinsically disordered proteins (IDPs) and intrinsically disordered protein regions (IDPRs) exist as interconverting conformational ensembles, without a single fixed three-dimensional structure in vivo. The focus in the literature up to this point has been primarily on IDPs that are mostly or entirely disordered. Therefore, we have an incomplete understanding of the incidence and functional relevance of IDPRs in proteins that have regions of both order and disorder. This work explores these populations, by examining IDPRs in the Protein Data Bank (PDB) and in enzymes. By applying disorder prediction methods combined with an analysis of missing regions in crystal structure data, …

Blind And Visually Impaired Users Adaptation To Web Environments: A Qualitative Study, Raneem Saqr

USF Tampa Graduate Theses and Dissertations

Although much research exists on human behavior in online environments, research on users with disabilities is still rare. To draw more attention to this population, this dissertation explored browsing patterns and adaptive behaviors of people with visual disability across different online environments common in daily activities: social network, e-commerce, online information, and search engines’ websites. The main objective of this study is to propose a conceptual framework of how blind and visually impaired users browse and adapt to different web environments. We achieve this objective using a qualitative approach through three studies. In the first study, the researchers collect data …

A New Materialist Approach To Visual Rhetoric In Photoshopbattles, Jonathan Paul Ray

USF Tampa Graduate Theses and Dissertations

The purpose of this project is to examine the visual rhetoric of one online community. Drawing heavily from the work of Laurie Gries (2015), I track the evolution of an image as it circulates through a forum of photo manipulators in the group “PhotoShopBattles.” While Gries’ work traced the evolution of the iconic Obama Hope poster and its iterations in various media, this project restricts its observations to the images posted to one webpage, focusing on one evolutionary chain. By narrowing the focus to one internet forum page that evolved over the course of one week, we can observe linear …

Msspark: Implementation Of Molecular Simulation Queries Using Apache Spark, Parneet Kaur

USF Tampa Graduate Theses and Dissertations

Huge amount of data is being generated in almost every field and it cannot be avoided, rather is essential for the advancement of the field. Analysis of this data requires intensive computing power. Molecular Simulation is a powerful tool for understanding the behavior of natural systems. The simulation generates large amount data while observing the spatial and temporal relationships. The challenge is to handle the analytical queries that are often compute intensive.

Although various tools exist to tackle this problem, but in this paper we have tried an alternate approach that uses Apache Spark- a modern big data platform – …

Inquiry Of Lipid Membranes Interacting With Functional Peptides And Polyphenol Drug Molecules, Chian Sing Ho

USF Tampa Graduate Theses and Dissertations

Cellular membranes are important targets for many membrane-active peptides and drug compounds. Here we are interested in deciphering how lipid membranes are perturbed by several membrane-active molecules, including the transmembrane domain of the influenza M2 protein (M2TM), aggregates formed by a synthetic polyglutamine peptide, and three polyphenol compounds (i.e., tamoxifen, genistein, and verapamil). We employ phase-separated ternary lipid model membranes in the form of giant unilamellar vesicles (GUVs) to simulate raft-like structures that have been proposed to govern many important processes in plasma membranes (e.g., intracellular singling and trafficking). Specifically, we use fluorescent microscopy to interrogate how those membrane additives …

Use Of A Game-Based App As A Learning Tool For Students With Mathematics Learning Disabilities To Increase Fraction Knowledge/Skill, Orhan Simsek

USF Tampa Graduate Theses and Dissertations

The aim of this study was to investigate the effectiveness of a game-based app (Motion Math: Fraction) to help students with Mathematics Learning Disabilities (MLD) to gain fraction skills including comparison, estimation, and word problem solving in an after school program. The researcher used multiple baseline design by extending with follow-up phase to determine whether students retained the knowledge they learned while engaging with the app. Even though six students participated to the study, the researcher withdrew two of them and analyzed data came from four students. The result o the study showed that all of the students …

A Power Conflict Approach To Animal Cruelty: Examining How Economic Power Influences The Creation Of Animal Cruelty Laws, Leonard J. Genco

USF Tampa Graduate Theses and Dissertations

The current study examines the association of macro-level economic factors and the creation or enactment animal cruelty laws across the states at a fixed time. Criminologists have postulated that economic factors influence the legal system (Chambliss and Seidman, 1971). This thesis addresses whether state-level economic and related macro-level factors influences the amount and types of state animal cruelty legislation lawmakers enact. To do so, this thesis examined animal cruelty legislation and their association with measures of agricultural and farm production, Democratic Party, Republican Party, and pro-animal interest groups across all states of the United States for the time period (2012-2013). …

Optimizing Methods For Extraction Of Organic Compounds From Molluscan Shells, Kaydee Jo West

USF Tampa Graduate Theses and Dissertations

Mollusk shells contain proteins within and between the crystals of calcium carbonate. These organic molecules play an important role in biomineralization and shell function, and their stable isotope ratios of carbon and nitrogen are also thought to record important ecological information about the animal's diet and nutrient sources. These proteins can be preserved for millions of years, offering potential insight into pre-anthropogenic ecological conditions. However, shell organics in older shells are typically recovered in reduced abundances due to leaching and remaining organics are often converted from insoluble proteins to soluble, free amino acids, making them difficult to detect and recover. …

“A Wound That Never Heals”: Health-Seeking Behaviors And Attitudes Towards Breast Cancer And Cancer In General Among Women In Nakirebe, Uganda, Ann Louise Tezak

USF Tampa Graduate Theses and Dissertations

The scale and severity of cancer, specifically breast cancer, remains significantly different across the spectrum of low-income to high-income countries. This study explores women’s beliefs about breast cancer and associated prevention and health-seeking behaviors in a rural area of Uganda. Through a critical medical anthropological perspective, the study examines the social, cultural, and economic factors that shape women’s understanding of cancer, and breast cancer specifically, and that influence their use of biomedical services. Data were collected over a three-month period through 35 in-depth interviews and two focus groups with 10 women older than 18 years in the rural setting of …

Development And Validation Of A Remote Sensing Model To Identify Anthropogenic Boreholes That Provide Dry Season, Refuge Habitat For Anopheles Vector Mosquitoes In Sub-Saharan Africa, James Pkemoi Kukat

USF Tampa Graduate Theses and Dissertations

A lack of surveillance systems is an impediment to public health intervention for perennial vector-borne disease transmission in northern tropical savanna region of Kenya. The population in this area are mostly poor nomadic pastoralists with little acquired functional immunity to Plasmodium falciparum, due to infrequent challenges with the parasite. A common characteristic in tropical savanna climatic zone is the availability of riverbeds that have anthropogenic boreholes that provide malaria vector mosquitoes, such as Anopheles gambiae s.l and Anopheles funestus, with aquatic refuge habitats for proliferation and endemic transmission to proximity human households during the dry-season. Unfortunately, currently there …

The Impact Of Latency Jitter On The Interpretation Of P300 In The Assessment Of Cognitive Function, Xiaoqian Yu

USF Tampa Graduate Theses and Dissertations

When stimuli processing time varies in an oddball paradigm, the latency of the P300 will vary across trials. In an oddball task requiring difficult response selections, as the variation of stimuli processing time increases, so does the variation of the P300 latency, causing latency jitters in the measurement. Averaging the P300 across different trials without adjusting this latency jitter will lead to diminished P300 amplitude, resulting in inaccurate conclusions from the data. Verleger et al. (2014) reported a diminished P300 amplitude in a difficult oddball task that required subjects to make response selections among stimuli that are difficult to distinguish, …

Inhibiting The Interaction Between Grp94 And Myocilin To Treat Primary Open-Angle Glaucoma, Andrew Stothert

USF Tampa Graduate Theses and Dissertations

Glaucoma is a neurodegenerative protein misfolding disorder classified by increases in IOP, damage to retinal ganglion cells (RGCs), optic nerve (ON) head damage, and progressive irreversible blindness. Primary open-angle glaucoma (POAG) is the most common form of glaucoma, constituting over 90% of clinical cases. POAG is observed in patients where normal outflow channels, mainly the trabecular meshwork (TM), are exposed at the angle formed by the iris and cornea. However, due to TM cellular dysfunction, aqueous outflow resistance is increased preventing normal circulation of aqueous humor. Recent studies have shown that in 2-4% of POAG cases, increased intracellular levels of …

The Restrictive Deterrent Effect Of Warning Banners In A Compromised Computer System, Christian Jordan-Michael Howell

USF Tampa Graduate Theses and Dissertations

System trespassing, which refers to the unauthorized access of computer systems, has rapidly become a worldwide phenomenon. Despite growing concern, criminological literature has paid system trespassing little attention. The current study utilizes data gathered from a Chinese computer network to examine system trespasser behavior after exposure to one of three warning messages: an altruistic message used for moral persuasion (warning 1), a legal sanction threat (warning 2), and an ambiguous threat (warning 3). More specifically, the current study examines the temporal order of various keystroke commands to determine if some keystroke commands are used as a tactical skill to avoid …

Global Attractors And Random Attractors Of Reaction-Diffusion Systems, Junyi Tu

USF Tampa Graduate Theses and Dissertations

The dissertation studies about the existence of three different types of attractors of three multi-component reaction-diffusion systems. These reaction-diffusion systems play important role in both chemical kinetics and biological pattern formation in the fast-growing area of mathematical biology.

In Chapter 2, we prove the existence of a global attractor and an exponential attractor for the solution semiflow of a reaction-diffusion system called Boissonade equations in the L² phase space. We show that the global attractor is an (H, E) global attractor with the L^∞ and H² regularity and that the Hausdorff dimension and the …

Pollutants And Foraminiferal Assemblages In Torrecillas Lagoon: An Environmental Micropaleontology Approach, Michael Martinez-Colon

USF Tampa Graduate Theses and Dissertations

Torrecillas Lagoon in the North Coast of Puerto Rico has experienced extensive anthropogenic influence over the past 400 years. Elevated concentrations of Potential Toxic Elements (PTEs) have been reported in surficial sediments. The main goal of this dissertation was to implement in Puerto Rico the use of benthic foraminifers as a bioindicators of PTEs and to compare the impact of Cu(II) on field samples with results of experimental work using cultures.

Analyses included geochemical assessment for bulk and carbonate- soluble bioavailable concentrations of PTEs in surface, core and pore-water samples, as well as analyses of grain-size, Percent Total Organic Carbon …

Statistical Analysis And Modeling Health Data: A Longitudinal Study, Bhikhari Prasad Tharu

USF Tampa Graduate Theses and Dissertations

Lung cancer has been considered one of the leading causes of deaths while cancer re- mains the second most common cause of deaths in the USA. Understanding the behavior of a disease over time could yield important information to make decisions about the disease. Statistical models could provide crucial clues and help to make a decision about the dis- ease, budget allocation, evaluation, and implement prevention. Longitudinal trend analysis of the diseases helps to understand long term effects and nature. Cholesterol level is one of the most contributing risk factors for Coronary Heart Disease. Studying cholesterol statistically helps to know …

Cmg Helicase Assembly And Activation: Regulation By C-Myc Through Chromatin Decondensation And Novel Therapeutic Avenues For Cancer Treatment, Victoria Bryant

USF Tampa Graduate Theses and Dissertations

The CMG (Cdc45, MCM, GINS) helicase is required for cellular proliferation and functions to unwind double-stranded DNA to allow the replication machinery to duplicate the genome. Cancer cells mismanage helicase activation through a variety of mechanisms, leading to the potential for the development of novel anti-cancer treatments. Mammalian cells load an excess of MCM complexes that act as reserves for new replication origins to be created when replication forks stall due to stress conditions, such as drug treatment. Targeting the helicase through inhibition of the MCM complex has sensitized cancer cells to drugs that inhibit DNA replication, such as aphidicolin …

Determining The Effectiveness Of Soil Treatment On Plant Stress Using Smart-Phone Cameras, Anurag Panwar

USF Tampa Graduate Theses and Dissertations

Plants are vital to the health of our biosphere, and effectively sustaining their growth is fundamental to the existence of life on this planet. A critical aspect, which decides the sustainability of plant growth is the quality of soil. All other things being fixed, the quality of soil greatly impacts the plant stress, which in turn impacts overall health. Although plant stress manifests in many ways, one of the clearest indicators are colors of the leaves. In this thesis, we conducted an experimental study in a greenhouse for detecting plant stress caused by nutrient deficienceies in soil using smartphone cameras, …