Digital Commons Network^™

Mir155, Triplicated In Down Syndrome, Regulates The Development Of Neural Stem Cells And Gabaergic Interneurons In Alzheimer's Disease Mouse And Human Ipsc Models., Xiaodong Zhu, Jean-Vianney Haure-Mirande, Mesude Bicak, Pengfei Dong, Ilya Kruglikov, Aiqun Li, Aisha Al-Subaie, Valentina Fossati, Scott Noggle, Sam Gandy, Michelle E Ehrlich

Faculty Research 2026

INTRODUCTION: Dysfunctional microRNAs and GABAergic interneurons are features of Alzheimer's disease (AD). The role of neuronal microRNA155 (miR155), elevated in both AD and Down syndrome (DS), remains unknown.

METHODS: We utilized in silico analyses of published databases, MIR155-deleted and -overexpressing human induced pluripotent stem cell (hiPSC)-derived cells, cortical organoids, and amyloid beta precursor protein (APP)/PS1-miR155 knockout mouse.

RESULTS: MIR155HG (miR155 host gene) colocalizes with APP in a neuron-specific, topologically associated domain (TAD) in chromosome 21. In human neural stem cells (NSCs), neurons, and cortical organoids, MIR155 deletion enhanced NSC proliferation and GABAergic interneuron generation. MIR155 overexpression inhibited NSC marker expression …

The Past, The Present, And The Future Of Preclinical Mouse Models For Alzheimer's Disease And Related Dementias., Adrian L Oblak, Michael Sasner, Gregory W. Carter, Gareth R Howell, Stacey J Sukoff Rizzo, Karina Leal, Paul R Territo, Bruce T Lamb

Faculty Research 2026

Over the past decade, the Model Organism Development and Evaluation for Late-Onset Alzheimer's Disease (MODEL-AD) consortium has transformed preclinical Alzheimer's disease (AD) research by addressing critical limitations in traditional mouse models that failed to translate to human disease. By leveraging human genetic discoveries, MODEL-AD has developed > 70 genetically informed mouse models, standardized phenotyping pipelines, and an open-access data infrastructure aligned with late-onset AD biology. These models incorporate human risk variants, environmental factors, and aging to better capture disease complexity, including emerging recognition of mixed pathologies such as vascular contributions, Lewy body disease, and TDP-43 proteinopathy. Despite substantial progress, key challenges …

Stromal Cell Senescence Augments Haematopoietic Cell Fitness In Clonal Haematopoiesis., Jayna J Mistry, Kira Young, Anna Navarro Figueredo, Gibran Edun, Alicia G Aguilar-Navarro, Patricia A Colom Díaz, Maria Telpoukhovskaia, Inés Fernández Maestre, Sheng F Cai, Katharina S Götze, Anastasia N Tikhonova, Ross L Levine, Jennifer J. Trowbridge

Faculty Research 2026

Microenvironment remodelling impacts tumour growth and metastasis, but whether remodelling promotes pre-malignant clonal fitness remains unknown. Here, using single-cell RNA-sequencing of the bone-marrow microenvironment in a mouse model of DNMT3A-mutant clonal haematopoiesis (CH), we identify mesenchymal stromal cells (MSCs) in a molecular state of cellular senescence. Elevated bone-marrow MSC senescence is also observed in humans with CH driven by several common somatic mutations. MSC senescence is induced by mutant haematopoietic cells in a contact-independent manner through production of soluble factors including TNF-α and IL-6. These cytokines activate a Stat3-driven pathway that is necessary and sufficient for MSC senescence induction. Genetic …

A Collaborative Framework For Uncovering Molecular And Cellular Drivers Of Vcid: Foundations For Future Interventions In Dementia., M Luisa Iruela-Arispe, Jason D Hinman, Andrew C Yang, Fabrice Dabertrand, Jin-Moo Lee, Gareth R Howell, The Vcid Centers Without Walls Network

Faculty Research 2026

Vascular-related factors are now considered major contributors to most forms of dementia, including Alzheimer's disease. However, the degree to which vascular deficits contribute to risk, onset, and progression of cognitive impairment and dementia has only recently been appreciated. Our understanding of the mechanisms by which vascular deficits drive cognitive decline in dementia is still limited. Further, the testing of therapeutic approaches to prevent vascular deficits to treat dementia are few. These factors motivated the establishment of the Vascular Contributions to Cognitive Impairment and Dementia (VCID) Center Without Walls (CWOW) network, comprising institutes across the United States. We describe the current …

Worldwide Innovative Network Consortium: Building A Common Global Cancer Database., Farhood Farahnak, Wafik S El-Deiry, Yves A Lussier, Razelle Kurzrock, Shai Magidi, Catherine Bresson, Shirin A Enger, Jia Liu, Jair Bar, Jeremy L Warner, Tobias Meissner, Eitan Rubin, Jens Rueter, Himabindu Gaddipati, Mandar Kulkarni, Zhen Chen, Sewanti Limaye, Rachel Elsey, Brenda M Rubenstein, Anthony M Joshua, Humaid O Al-Shamsi, Khaled M Musallam, Fanny Wunder, Jacques Raynaud, Guy Berchem, Manon Gantenbein, Amal Al Omari, Said Dermime, Hikmat Abdel-Razeq, Pierre Saintigny, Andrés Cervantes, Roger R Reddel, Adel T Aref, Juan Martin-Liberal, Conxi Lázaro, David Cordero Romera, Marina Sekacheva, Raanan Berger, C S Pramesh, Ioana Berindan-Neagoe, Eugenia Girda, Alejandro Piris-Gimenez, Carol J Farhangfar, Mohammed E Salem, Rodrigo Dienstmann, Ramon Salazar, Naftali Z Frankel, Zachary Batist, Yuri Quintana, Gerald Batist

Faculty Research 2026

This review shares the ongoing work of the global Worldwide Innovative Network (WIN) Consortium for Precision Medicine to synthesize emerging cancer treatment data and to define the requirements for a common global cancer database that can truly support precision oncology. We performed a narrative review of emerging cancer treatment data, molecular profiling technologies, and existing clinicogenomic databases, focusing on how tumors are characterized, how subgroups are defined, and how demographic, lifestyle, and environmental factors are captured. The growth in molecular profiling technologies and the development of new targeted therapies are transforming cancer care. Tumors, regardless of tissue origin, are increasingly …

Inhibition Of Moesin And Cd44 In Stem Cell-Derived Neurons Affects The Pathological Genetic Signature Associated With Alzheimer's Disease., Eiden H Brewer, Charles A Williams, Gregory Cary, Ranjita Betarbet, Inez K A Pranoto, Elizabeth L Zoeller, Haian Fu, Allan I Levey, Jesse C Wiley, Gregory W. Carter, Jessica E Young, Treat-Ad Consortium

Faculty Research 2026

INTRODUCTION: Post mortem proteomic analysis of Alzheimer's disease (AD) brain tissue has identified novel target genes and proteins for potential therapeutic development. Moesin (MSN) and CD44 were identified as candidate targets. Using human induced pluripotent stem cells (hiPSCs)-derived neurons, we assayed how reducing gene expression of MSN and CD44 affected amyloid beta secretion, tau phosphorylation, and transcriptional state.

METHODS: Knockdown of MSN and CD44 in hiPSC-derived neurons was performed using short hairpin RNA (shRNA). Amyloid precursor protein processing and intracellular tau phosphorylation was measured using chemiluminescent ELISA assays. Global gene expression was analyzed by bulk RNA sequencing (RNA-seq).

RESULTS: Knockdown …

Educator Experiences Of Schoolwide Pbis Discipline Implementation: Exploring School Culture In Private Pk–12 Schools, Jessica L. Guarneri

Electronic Theses and Dissertations

The purpose of this phenomenological study was to explore educators’ lived experiences implementing schoolwide Positive Behavioral Interventions and Supports (PBIS) discipline systems and to understand how those experiences influenced school culture in private PK–12 schools. Historically, PBIS focused on specific outcomes, such as reductions in discipline referrals, suspensions, and behavioral incidents. Though these indicators are critical, they do not consider in what way educators experience disciplinary systems, nor how their lived experiences affect routine interactions with students. Educators play a key role in implementing PBIS; their experiences foster consistency, trust, and sustainability. The participants included certified educators and administrators employed …

A Qualitative Examination Of Tennessee Secondary Stem Teachers’ Perceptions On Professional Development Needs, Amber W. Ogle

Electronic Theses and Dissertations

The purpose of this qualitative phenomenological study was to research secondary STEM educators’ perceptions of PD needs. This study focused on the lived experiences of educators actively teaching in STEM subject disciplines in grades nine through 12. The essential question for this research study was: How do Tennessee secondary STEM teachers perceive the relevance, applicability, and impact of PD opportunities? The research was rooted in Knowles’ (1984) theory of adult learning, Andragogy, and Learning Forward’s (2022) Standards for Professional Learning.

Data were collected through 15 semi-structured, one-on-one online interviews. The transcripts of these interviews were analyzed, and frequently used language …

Analyzing The Effects Of Biodegradable Plastic Fragments On Maize Development And Defense Signaling, Ulysses Hampton

Theses

As plastic use shifts from non-degradable to biodegradable materials, the effects of biodegradable plastic fragments on crop physiology remain unclear. This study investigated how biodegradable plastic fragments influence maize development, transpiration, and defense signaling. Among five plastic types tested, poly(butylene adipate-co-terephthalate) (PBAT), polyhydroxyalkanoates (PHA), polycaprolactone (PCL), polylactic acid (PLA), and thermoplastic starch (TPS). PBAT and TPS showed the most significant effects and were selected for further oxylipin analysis. Results indicate that biodegradable plastic fragments can alter oxylipin signaling pathways in subtle but meaningful ways, suggesting that these materials are not inherently risk-free alternatives to conventional plastics.

Design Of A Continuum Robot Test Bed Utilizing Model-Free Sliding Mode Control, Joseph Paul Streib

Theses

Continuum robots are a class of continually deformable robots inspired by snakes and tentacles that are commonly used in medical, search and rescue, and exploration applications. The robots are highly nonlinear and difficult to accurately model and control. Precise control of the pose of a continuum robot is typically reliant upon a system model. This work introduces a novel model free control algorithm for continuum robots that only relies upon the robot’s position in task space. The derived controller is built upon the foundation of a preexisting model free sliding mode controller and the q-state parameterized piecewise constant curvature kinematic …

Engineering Materials Function With Reconfigurable Colloids, Laura Galeano Tirado

Theses

The function of a material is defined not only by its chemical composition, but also by the structural organization of its constituents across multiple length scales, from the architecture of individual building blocks to their collective arrangement. Developing strategies to access, engineer, and reconfigure that structure on demand represents a central opportunity in the design of adaptive materials. Here, we present a thermoresponsive polymer-colloid platform that enables high-precision tuning of colloidal structures using only thermal stimuli. Rather than adsorbing to the surface, our polymers diffuse inside organosilica colloids to reshape them from within, allowing continuous modulation of their size, shape, …

Semiconductor Laser Calibration Through Photonic Chip With Microcontroller Driven Wavelength Inference, Clayton Acheson

Theses

Integrated Silicon Photonics leverages light-speed operation with lower power and higher data rates than electronic counterparts. To achieve this, light is sent through a silicon circuit, where it is manipulated to perform various operations. Primarily, the light for these circuits comes from laser sources. Semiconductor lasers are typically used for their small form factor and ability to be heterogeneously integrated into larger, more complex systems. Because the optical phase and wavelength of light respond to minute changes in local refractive index, light is increasingly used for high-sensitivity sensing in integrated photonic circuit applications. As such, schemes that integrate the laser …

Finding Balance: School-Based Yoga Interventions And Their Alignment Within Multi-Tiered Systems Of Support, Ursula Vogt

Educational Specialist, 2020-current

School-based interventions that support emotional regulation and student well-being are increasingly emphasized within Multi-Tiered Systems of Support (MTSS). Yoga has emerged as a potential school-based practice; however, its outcomes, alignment with MTSS frameworks, and implementation characteristics remain unclear. This systematized review examined (a) psychological, emotional, and behavioral outcomes associated with school-based yoga interventions, (b) their alignment with Tier 1 and Tier 2 supports, and (c) implementation characteristics that support feasibility in school settings. A systematic search identified 21 U.S.-based studies, which were analyzed using thematic analysis. Findings indicated that yoga interventions were associated with improvements in emotional regulation, stress and …

Cellular Senescence Links Muscle Atrophy And Posttraumatic Osteoarthritis After Acl Injury, Alexander R. Keeble, Allison Owen, Sara Gonzalez-Velez, Nicholas T. Thomas, Camille R. Brightwell, Peyton J. Balawender, Madeline G. O'Daniel, Helena C. Weiss, Haley Noehren, Heather Thompson, Karen Sofia Zuluaga-Osorio, Zane T. Bates, Lauren Gottlieb, Douglas E. Long, Douglas Harrison, Philip Kern, Caitlin E. Conley, Michael A. Samaan, Stephen Duncan, Austin Stone, Darren Johnson, Yuan Wen, Brian Noehren, Cory Dungan, Christopher Fry

Biology Faculty Publications

Traumatic knee injury leads to posttraumatic osteoarthritis (PTOA) and significant skeletal muscle weakness, resulting in chronic disability. The current standard of care frequently fails to prevent musculoskeletal dysfunction, underscoring the need to identify therapeutic mechanisms of PTOA. Using an established preclinical anterior cruciate ligament (ACL) transection model of PTOA and leveraging an innovative SPiDER-senescence-associated β-galactosidase stain to discern senescent cells, we investigated cellular senescence at single-cell resolution and identified anti-inflammatory macrophages as a predominant contributor to the senescent cell burden in both muscle and knee joint after injury. Clearance of senescent cells using the senolytic dasatinib and quercetin (D + …

Crystal Structure Of New Polar Rare Earth Borates Na2.62Ln2.12(Bo3)3 (Ln = Pr, Nd, Sm) Containing Isolated Bo3 Groups, Alevtina A. Maksimova, Mark D. Smith, Hans Conrad Zur Loye

Faculty Publications

Three new rare earth borates, Na2.62Ln2.12(BO3)3 (Ln = Pr, Nd, Sm), were synthesized via a high-temperature solution reaction using a BaCO3–H3BO3–NaF flux and structurally characterized by single-crystal X-ray diffraction analysis. The compounds crystallize in the orthorhombic space group Amm2 with lattice parameters a = 5.0985(10) Å, b = 11.180(2) Å, c = 7.1483(14) Å, and a unit cell volume of 407.48(14) ų (Z = 2) for Na2.62Pr2.12(BO3)3, a = 5.0983(10) Å, b = 11.181(2) Å, c = 7.1491(14) Å, and a unit cell volume of 407.51(14) ų (Z = 2) for Na2.62Nd2.12(BO3)3, and a = 5.08630(10) Å, b …

Single Crystal Growth Of The Orthoborate Nabalu(Bo3)2 And Optical Investigation Of Eu3+ And Tb3+ Doped Nabalu(Bo3)2, Alevtina A. Maksimova, Mark D. Smith, Lakshani W. Masachchi, Hans Conrad Zur Loye

Faculty Publications

Sodium barium lutetium borate NaBaLu(BO₃)₂ crystals were synthesized using a high-temperature solution method. The crystal structure of this layered orthoborate is described and corresponds to that of an extensive orthoborates family, NaBaR(BO₃)₂ (R = Sc, Y, Yb, Tb – Lu). NaBaLu(BO₃)₂ was doped by Eu³⁺ and Tb³⁺ to explore its performance as a host material and to investigate the luminescent properties of the Eu³⁺ and Tb³⁺ doped materials. The emission spectra of the NaBaLu(BO₃)₂:Ln (Ln = Eu, Tb) crystals are presented. The most …

The True Scope Of Trusting Your Gut: A Study Into The Enteric Nervous System And The Relationship Between The Gut-Brain Axis And The Mind, Bryan Pham

Theses & ETDs

Since the early days of medicine, the “gut” had been associated as a seat of emotions and intuition, but as modern science marched forward, the brain itself was given complete dominion over mental health. But thanks to research into the enteric nervous system (ENS), which innervates the digestive tract and bilaterally communicates with the brain as a secondary function, we now know that, in a sense, the gut does indeed have a substantial impact on mental health outside of its primary functions. This profound discovery could potentially cause a massive rewrite for decades of modern neuroscience-based medicine. Overall, the study …

Molecular Refinement Of The Taxonomy Of Pyrgulopsis Springsnails In Western North America, Michael K. Young, Kristine L. Pilgrim, Eric S. Miskow, Chante L. Lundskog, Kathryn E. Perez, Michael K. Schwartz

School of Integrative Biological & Chemical Sciences Faculty Publications

Springsnails in the genus Pyrgulopsis in western North America have long been a dilemma for taxonomists. Defining species boundaries has been complicated by the presence of undiscovered or cryptic taxa, range over- or underestimation, species complexes representing an unknown number of species, and named species synonymous with other taxa. To address these issues, we conducted an array of molecular species delimitation analyses using sequences from up to 2 mitochondrial and 2 nuclear genes from over 5000 specimens representing 140 of the 151 currently recognized taxa. These analyses reaffirmed most species hypotheses, favored synonymy for some taxa and redrawing of species …

Predictors Of The Mother-Infant Bond: The Role Of Maternal Attachment Style, Parity, Race, And Motherhood Expectations, Samia Qadri

Theses, Dissertations and Culminating Projects

This study examined how maternal attachment style, parity, race, and motherhood expectations, individually and in interaction, predicted the quality of the mother-infant bond during the postpartum period. Grounded in a maternal feminist framework, the study used a quantitative, cross-sectional correlational design. Postpartum mothers aged 20–39 (N = 87) completed validated measures of maternal attachment style, motherhood expectations, mother-infant bonding, and depressive symptoms. Multiple regression compared a Primary Model containing the four focal predictors and planned interaction terms with a Secondary Model that added maternal depressive symptoms as a covariate. Depressive symptoms emerged as the strongest single predictor of bonding, and …

Validating The Expression Of Genes Potentially Involved In The Development Of Aggressive Melanoma Tumor In The Zebrafish Melanoma Model, Mayra Matos Diaz

Theses, Dissertations and Culminating Projects

Melanoma is an aggressive form of skin cancer characterized by rapid progression, metastatic potential, and the frequent development of resistance to targeted therapies. Although the Inducible cAMP Early Repressor (ICER) has been identified as a potential tumor suppressor in melanoma, the molecular mechanisms underlying its role in tumor progression remain poorly understood. Building upon the transcriptomic findings of Wheelan et al. (2025), this study investigates the relationship between ICER and ten candidate genes (gdf6a, pdgfra, col4a4, fgfr2, pdgfrb, cdkn1a, fgf5, akt1, met, and notch3) associated with BMP signaling. Quantitative polymerase chain reaction (qPCR) was used to compare gene expression between …

Temperature And Salinity Effects On The Physiology Of Bullfrog Tadpoles (Lithobates Catesbeianus) From A Suburban Temperate Wetland, Angela Brown

Theses, Dissertations and Culminating Projects

One of the most robust amphibians is the American Bullfrog, Lithobates catesbeianus, which is a widespread species that is invasive worldwide, and is broadly tolerant of a range of environmental conditions. Despite this tolerance, survival and performance of adult L. catesbeianus can be negatively affected by increasing temperatures and salinity resulting from anthropogenic impacts, such as climate change and road salts. This study focuses on the effects of acute salinity exposure (mimicking road salt influx into a pond) on L. catesbeianus tadpoles at different larval stages. Increased salinity was predicted to negatively affect two physiological performance measures. Critical maximum temperature …

2026-08-06 Executive Council Minutes, Susie Salazar

Executive Council Minutes

No abstract provided.

High Biochar Rate Improves Water Retention In Some Environmentally Sensitive Soils, Samundra Sigdel

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Improving water retention and other hydraulic properties is key to managing soil water and sustaining crop production under fluctuating and extreme weather events. We assessed how wood biochar (836 g C kg^-1) applied at 0, 6.25, 12.5, 25, and 50 Mg ha^-1 affected: i) soil hydraulic properties after 5 years and ii) corn and soybean yield, and biomass production of cereal rye cover crop (CC) and CC mix (cereal rye, radish, and winter pea) in sandy, sloping, and semi-arid soils in the central U.S. Great Plains over 6 years. Biochar affected field saturated hydraulic conductivity (K_{sat …}

A Practical Guide To Science Writing & Publishing, Ryan W. Mcewan

Department of Biology Open Educational Resources

This document contains advice that I developed for students working in my research lab and represents an approach to science writing that is in some ways very basic, and in others, quite idiosyncratic.

Writing is a creative process and as you advance as a science writer you will get stronger, and stronger, and you will develop your own process. At some point, someone might give you different advice about science writing than what you find here. If you join a research lab your PI might have their own program. You should absorb all of the good advice you can find. …

At Risk Students: Ensuring A Successful Transition Into High School, Angela Espada

Education Theses and Dissertations

This Improvement Science Dissertation in Practice concentrated on the persistent problem of freshman course failure at a large, diverse, Title I high school serving a military-connected community in Central Texas. Although intervention programs existed, an increasing number of ninth-grade students were consistently failing one or more courses during their first year of high school, which revealed several gaps in the campus’s system of support. The Improvement Science framework and two Plan-Do-Study-Act (PDSA) cycles were used to examine the factors contributing to freshman course failure and to assess the interventions implemented to improve academic success as students transitioned into high school. …

Evaluating The Discus Throw As An Ancient Greek Combat Training Tool Through Modern Performance Metrics, Daniel R. Porter

All Graduate Reports and Creative Projects, Fall 2023 to Present

This study’s purpose was to explore the correlations between peak velocity in a rotational throw with a medicine ball, peak force (PF) and rate of force development (RFD) in an isometric mid-thigh pull, time to failure in a plank test, and peak velocity in a sword thrust task, which was also tested for reliability. Twenty-seven recreationally trained male participants between the ages of 18-35 completed the study. The sword thrust task was significantly correlated with the medicine ball throw (p = 0.012, r = 0.475, r² = 0.226, 95% CI [0.724, 0.116]) and PF (p = 0.045, r …

Economic Efficiency Of End-Guns On Center Pivot Irrigation Systems For Utah Alfalfa Production, Sophia Moss

All Graduate Reports and Creative Projects, Fall 2023 to Present

This study looks at whether using an end-gun on a center pivot irrigation system is financially worthwhile for Utah alfalfa producers. End-guns are used to water extra acreage beyond the end of a pivot, they can also apply water less evenly, which may reduce production on those outer acres. To examine this tradeoff, the study compares farm returns with and without an end-gun using historical Utah alfalfa price and yield data along with estimates of irrigation cost, added acreage, and possible yield loss. The results suggest that end-guns can improve profitability in many cases, mainly because they bring more acres …

A Social-Ecological Systems Approach To Integrating Outdoor Recreation And Black Bear Management: A Michigan Case Study, Megan Dorothy Stone

All Graduate Theses and Dissertations, Fall 2023 to Present

Outdoor recreation sits at the intersection of social and ecological systems, representing one of the primary ways in which humans directly interact with their natural environment. As a result, outdoor recreation management must balance providing high-quality outdoor recreation opportunities while simultaneously protecting natural resources in protected areas. Balancing these concurrent management goals has become more challenging in recent years as participation in outdoor recreation increases. This can lead to numerous negative impacts on the environment, such as wildlife disturbance. In this study, I explore the challenges and opportunities for collaboration between managers of outdoor recreation and black bears in the …

Teachers’, Teacher Assistants’, And Classroom Aides’ Perception, Knowledge, And Implementation Of Orton-Gillingham, Doreen Sisolak

Seton Hall University Dissertations and Theses (ETDs)

This study examined teachers’, teacher assistants’, and classroom aides’ perceptions, knowledge, and implementation of the Orton–Gillingham approach to reading instruction. A mixed-methods design was employed, incorporating quantitative survey data (N = 37) and qualitative interview data (N = 9). Descriptive statistics indicated generally low levels of perception, knowledge, and implementation among participants. One-way analyses of variance (ANOVA) were conducted to examine differences based on instructional setting, years of experience, and level of Orton-Gillingham training. Results revealed statistically significant differences in knowledge and implementation by training level and years of experience, whereas no significant differences were found in perceptions across groups. …

The Well-Being Of California School Board Members, Lamiya A. Hoque

Education (PhD) Dissertations

The primary purpose of this study was to examine the well-being of California school board members. Using an adapted Workplace PERMA-Profiler, a quasi-experimental study was conducted with 144 school board members to investigate the study's research questions. Pearson correlation analyses and hierarchical multiple regression were used to examine the relationships between overall well-being and the Workplace PERMA-Profiler domains. Differences in overall well-being and PERMA domain scores across age groups and years of service were examined using one-way analyses of variance with Tukey HSD post hoc comparisons. Results indicated that each PERMA domain was positively associated with overall well-being, whereas loneliness …