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Levels Of Distress Among Women Veterans Attending A Women’S Health Specialty Clinic In The Va Healthcare System, Debbie T. Devine

USF Tampa Graduate Theses and Dissertations

Currently, between 21.9 and 23 million veterans have served in the United States armed forces. Of those, 2 million are women, and of those, only 6.5% use the Veterans Health Administration system. These females often suffer from physical and mental health disorders, and overall impaired quality of life (QOL), rendering their healthcare needs complex. Seeking, and providing care in this specialty area may become overwhelming not only for the women seeking the care, but also for healthcare systems that are unfamiliar with the specific needs of this population.

A retrospective medical records review was completed of 51 female veterans between …

Significance Of Pten Phosphorylation And Its Nuclear Function In Lung Cancer, Prerna Malaney

USF Tampa Graduate Theses and Dissertations

Phosphorylation mediated inactivation of PTEN leads to multiple malignancies with increased severity. However, the consequence of such inactivation on downstream functions of PTEN are poorly understood. Therefore, the objective of my thesis is to ascertain the molecular mechanisms by which PTEN phosphorylation drives lung cancer. PTEN phosphorylation at the C-terminal serine/threonine cluster abrogates its tumor suppressor function. Despite the critical role of the PTEN C-tail in regulating its function, the crystal structure of the C-tail remains unknown. Using bioinformatics and structural analysis, I determined that the PTEN C-tail is an intrinsically disordered region and is a hot spot for post-translational …

A Framework For Studying Meshfree Geometry And A Method For Explicit Boundary Determination, Joseph Bradley Alford

USF Tampa Graduate Theses and Dissertations

Patient-specific biomechanical analysis is an important tool used to understand the complex processes that occur in the body due to physical stimulation. Patient-specific models are generated by processing medical images; once an object from the image is identified via segmentation, a point cloud representation of the object is extracted. Generating an analysis suitable representation from the point cloud has traditionally required generating a finite element mesh, which often requires a well defined surface to accomplish. Point clouds lack a well defined geometry, meaning that the surface definition is incomplete at best. Point clouds that have been generated from images have …

Hydrolytic And Nonhydrolytic Sol-Gel Zirconia-, Titania-, And Niobia-Based Capillary Microextraction Coatings For The Preconcentration And Hplc Analysis Of Catecholamine Neurotransmitters And Phosphorylated Peptides, Abdullah Awadh Alhendal

USF Tampa Graduate Theses and Dissertations

Sample preparation is the most error-prone step in chemical analysis. A great deal of efforts has been made to develop efficient techniques and protocols for sample preparation to accomplish important goals such as miniaturization and implementation of green analytical methodologies. Solid-phase microextraction (SPME) has successfully eliminated the use of hazardous organic solvents in extraction sampling, sample preparation, preconcentration and sample introduction to the analytical instrument in an effective manner. Ensuring thermal- and solvent-instability of traditional SPME extraction phases represented one of their main drawbacks. This was solved by the introduction of sol-gel SPME phases characterized by enhanced thermal-, solvent-, and …

Climate Change Drives Outbreaks Of Emerging Infectious Disease And Phenological Shifts, Jeremy Cohen

USF Tampa Graduate Theses and Dissertations

Climate change is expected to impact species by altering infectious disease outcomes, modifying community composition, and causing species to shift their phenology, body sizes and range distributions. However, the outcomes of these impacts are often controversial; for example, scientists have debated whether climate change will exacerbate emerging infectious disease and which species are at greatest risk to advance their phenology. There reason for these controversies may be that climate change is impacting diverse processes across a wide range of ecological scales, as the interplay between fine-scale processes and broad-scale dynamics can often cause unpredictable changes to the biosphere. Therefore, it …

Hypercapnic Hyperoxia Increases Free Radical Production And Cellular Excitability In Rat Caudal Solitary Complex Brain Slice Neurons, Geoffrey Edward Ciarlone

USF Tampa Graduate Theses and Dissertations

The caudal solitary complex (cSC) is a cardiorespiratory integrative center in the dorsal medulla oblongata that plays a vital role in the central CO₂-chemoreceptive network. Neurons in this area respond to hypercapnic acidosis (HA) by a depolarization of the membrane potential and increase in firing rate, however a definitive mechanism for this response remains unknown. Likewise, CO₂-chemoreceptive neurons in the cSC respond to hyperoxia in a similar fashion, but via a free radical mediated mechanism. It remains unknown if the response to increased pO₂ is merely an increase in redox signaling, or if it’s the …

“Way Down Upon The Suwanee River”: Examining The Inclusion Of Black History In Florida’S Curriculum Standards, William Newell

USF Tampa Graduate Theses and Dissertations

As education focuses increasingly on standards based assessment, social studies must be examined for its integration of Black History in the United States History curriculum. Using a Critical Race Theory lens, this directed content analysis attempts to examine the Florida Standards for United States History to determine if and how Black History is integrated into United States History courses. The study also makes use of Banks’ (1994) “levels of integration” to explore the degree to which this is accomplished. In addition, lesson plans created and/or endorsed by the state of Florida are analyzed for their inclusion of Black History. Data …

Novel Magnetic Nanostructures For Enhanced Magnetic Hyperthermia Cancer Therapy, Zohreh Nemati Porshokouh

USF Tampa Graduate Theses and Dissertations

In this dissertation, I present the results of a systematic study on novel multifunctional nanostructure systems for magnetic hyperthermia applications. All the samples have been synthesized, structurally/magnetically characterized, and tested for magnetic hyperthermia treatment at the Functional Materials Laboratory of the University South Florida. This work includes studies on four different systems: (i) Core/shell Fe/γ-Fe₂O₃ nanoparticles; (ii) Spherical and cubic exchange coupled FeO/Fe₃O₄ nanoparticles; (iii) Fe₃O₄ nano-octopods with different sizes; (iv) High aspect ratio FeCo nanowires and Fe₃O₄ nanorods.

In particular, we demonstrated the enhancement of the heating …

Regulation Of Palmitoylation Enzymes And Substrates By Intrinsically Disordered Regions, Krishna D. Reddy

USF Tampa Graduate Theses and Dissertations

Protein palmitoylation refers to the process of adding a 16-carbon saturated fatty acid to the cysteine of a substrate protein, and this can in turn affect the substrate’s localization, stability, folding, and several other processes. This process is catalyzed by a family of 23 mammalian protein acyltransferases (PATs), a family of transmembrane enzymes that modify an estimated 10% of the proteome. At this point in time, no structure of a protein in this family has been solved, and therefore there is poor understanding about the regulation of the enzymes and their substrates. Most proteins, including palmitoylation enzymes and substrates, have …

From Portraits To Selfies: Family Photo-Making Rituals, Krystal M. Bresnahan

USF Tampa Graduate Theses and Dissertations

From family-style portraits to selfies, who is photographer and/or photographed varies as families engage, stage, and interpret the visual. How families participate in photo-making changes how individual family members feel about and relate to not only their photographs, but also each other. In this dissertation, I examine photographs as visual and material objects, and include the communication processes and ritual practices of producing, consuming, curating, viewing, and circulating these photos. By framing family photo-making as ritual, I explore how families do photo-making in everyday life, and identify the patterns of choice embedded in the genre of family photography, which symbolically …

Constituting Agricultural And Food Policy In Malawi: The Role Of The State And International Donors In The Farm Input Subsidy Program (Fisp), Peter Rock Nkhoma

USF Tampa Graduate Theses and Dissertations

Numerous studies have been undertaken on the political economy of agricultural policies in developing countries. These studies have explained agricultural policies in terms of urban bias, economic reforms, and domestic politics. Recently, the emphasis has been on explanations that reference the existence of a rational-legal and patronage element within the African state. Such explanations tend to underplay the extent to which agricultural policies are devised in a context of power asymmetries between the state and international donors or financial institutions. In the Malawian context specifically, limited attention has been paid to the possibility that policies are a negotiated outcome of …

Development Of An Anaerobic-Phototrophic Bioreactor System For Wastewater Treatment, Onur Yilmaz Ozcan

USF Tampa Graduate Theses and Dissertations

For decades, mainstream domestic wastewater treatment has relied on activated sludge processes to remove organic matter, and on biological nutrient removal systems like the A²/O process to remove nutrients. Recently, membrane filtration was also added to the realm of possible technologies for domestic wastewater treatment, with aerobic membrane bioreactors (MBRs) becoming increasingly popular, especially for decentralized, and small to medium scale applications. However, the aerobic activated sludge and MBR processes, which are often combined with biological nutrient removal processes, have high energy costs associated with supplying oxygen to the process, and end up converting the organic matter into …

Influence Of Water Quality On Stony Coral Diversity And Net Community Productivity In The Florida Keys, María Vega-Rodriguez

USF Tampa Graduate Theses and Dissertations

Worldwide, coral cover has declined at rates that have often exceeded 5% per year since the 1980’s. Populations of scleractinians (stony corals) in the Florida Keys reef tract have declined as well, with some communities declining at rates > 3% per year. Decreased water quality (e.g., steady increases in the ocean water temperatures and increased pollution, nutrients, or water turbidity due to coastal runoff) are commonly attributed to this decline. But actual linkages between variability and trends in these environmental parameters, and in stony coral diversity and ecosystem functions such as net community production, have not yet been well characterized.

With …

The Role Of Religion In Mitigating Cancer Disparities Among Black Americans, Samar Hennawi

USF Tampa Graduate Theses and Dissertations

The long history of racism has created cultural barriers that prevent some Black Americans from seeking cancer treatment. Fatalism, physician mistrust, low levels of social support and self-efficacy, are the most cited cultural barriers in the literature. Black Americans’ religious beliefs and church involvement have historically helped them in their struggle against racism. A quantitative and a qualitative comparison studies examine the role of Black cancer survivors’ religious beliefs in their fight against cancer. The quantitative comparison study finds no difference in the cultural attitudes between Black and White cancer survivors. However, the qualitative comparison study between the same two …

An Ecosystem-Based Approach To Reef Fish Management In The Gulf Of Mexico, Michelle D. Masi

USF Tampa Graduate Theses and Dissertations

Fisheries managers have the potential to significantly improve reef fish management in the Gulf of Mexico through the use of ecosystem-based approaches to fisheries management. Ecosystem-based approaches are needed to address the effects of fishing on trophodynamic interactions, to better account for ecosystem-scale processes in model projections, and to recognize the short and long-term biomass tradeoffs associated with making regulatory choices. My research was concentrated around three objectives: (1) characterizing the trophodynamic interactions between Gulf of Mexico fishes, in order to construct an invaluable tool (a Gulf of Mexico Atlantis model) to be used in ecological hypothesis testing and policy …

Predictors Of Associate's Degree Completion In Engineering And Engineering Technologies, Lynsey Reys-Nickel

USF Tampa Graduate Theses and Dissertations

The purpose of this ex post facto study was to describe completers and non-completers of associate’s degree programs in engineering and engineering technologies and determine whether and to what extent completion in these programs is a function of selected student-related variables and institutional variables. Data from the 2004/2009 Beginning Postsecondary Students Longitudinal Study (BPS: 04/09) of associate’s degree completers and non-completers in engineering and engineering technologies were accessed and analyzed through PowerStats, a web-based data analysis tool from National Center for Education Statistics (NCES).

Descriptive data indicated that, proportionally, engineering and engineering technologies completers were mostly White, married, middle income, …

Intraocular Pressure Sensing And Control For Glaucoma Research, Simon Antonio Bello

USF Tampa Graduate Theses and Dissertations

Animal models of ocular hypertension are important for glaucoma research but come with experimental costs. Available methods of intraocular pressure (IOP) elevation are not always successful, the amplitude and time course of IOP changes are unpredictable and irreversible, and IOP measurement by tonometry is laborious. This dissertation focuses on the development and implementation of two novel systems for monitoring and controlling IOP without these limitations. The first device consists of a cannula implanted in the anterior chamber of the eye, a pressure sensor that continually measures IOP, and a bidirectional pump driven by control circuitry that can infuse or withdraw …

Hormone Replacement Therapy (Hrt) Modulates Peripheral And Central Auditory System Processing With Aging, Tanika Williamson

USF Tampa Graduate Theses and Dissertations

After the findings were reported for the Women’s Health Initiative (WHI) study in the past decade, there has been a significant decline in the overall use of hormone replacement therapy (HRT) among women. However, there are still millions of middle-aged, menopausal women in the U.S. who are currently undergoing hormone therapy. Their reasons for continuing treatment include relief of severe menopausal symptoms, aid in the management of osteoporosis and reduction in the risk of colon cancer (Ness et al., 2005). The purpose of the following investigation was to evaluate the impact of HRT on the central and peripheral auditory systems …

Molybdenum Disulfide-Conducting Polymer Composite Structures For Electrochemical Biosensor Applications, Hongxiang Jia

USF Tampa Graduate Theses and Dissertations

Lactic acid is widely existing in human bodies, animals and microorganisms. Recently, using biosensor to detect the concentration of lactic acid and diagnose disease have attracted great research and development interests. Nanocomposites is one of the best material used for biosensor because their wonderful conductivity, optical and electrochemical properties. In the study, MoS₂ and polypyrrole (PPY) are used for the composite material electrode. To determine whether lactate oxidase (LOD) was helpful for the biosensor’s detective properties, both PPY-MoS₂ film with LOD and PPY-MoS₂ film without LOD are being tested. The fourier transform infrared spectroscopy (FTIR) and Raman …

The Influence Of Oxygen Tension And Glycolytic And Citric Acid Cycle Substrates In Acrolein-Induced Cellular Injury In The Differentiated H9c2 Cardiac Cell Model, Jayme Coyle

USF Tampa Graduate Theses and Dissertations

Most in vitro systems employ the standard cell culture maintenance conditions of 95 % air with 5 % CO2 to balance medium pH, which translates to culture oxygen tensions of approximately 20 % - above the typical ≤ 6 % found in most tissues. The current investigation, therefore, aims to characterize the effect of maintenance and toxicant exposure with a particular focus on the α,β-unsaturated aldehyde, acrolein, in the presence of physiologically relevant oxygen tension using a differentiated H9c2 cardiomyoblast subclone. H9c2 cells were maintained separately in 20.1 and 5 % oxygen, after which cells were differentiated for five days, …

Pepper Mild Mottle Virus As A Surrogate For Enteric Viruses: Implications For Assessing Water Quality, Erin Michelle Symonds

USF Tampa Graduate Theses and Dissertations

Less than 10% of the world’s domestic wastewater is disinfected prior to discharge into surface waters; therefore, human exposure to diverse wastewater-related pathogens results in millions of cases of illness each year. Among the enteric pathogens, viruses represent an important group of emerging pathogens and are frequently the cause of food- and water-borne outbreaks of illness. Although the World Health Organization and many government agencies mandate the use of bacterial indicators to identify poor microbial water quality, it is well known that these indicators poorly correlate with fecal pollution contamination events and risk of disease. The field of public health-related …

Formation Of Trihalomethanes (Thms) As Disinfection By-Products (Dbps) When Treated Municipal Wastewater Is Disinfected With Sodium Hypochlorite, Helene Kassouf

USF Tampa Graduate Theses and Dissertations

Disinfection is an essential process in the treatment of municipal wastewater before the treated wastewater can be discharged to the environment. Hillsborough County's Northwest Regional Water Reclamation Facility (NWRWRF) in Tampa, Florida, currently uses ultraviolet (UV) light for disinfection. However, this method has proven expensive to implement and maintain, and may not be effective if the light transmission is poor. For these reasons, Hillsborough County is considering switching from UV light to sodium hypochlorite for disinfection. However, hypochlorite (chlorine) disinfection has disadvantages as well, such as the production of disinfection by-products (DBPs) such as trihalomethanes (THM) and haloacetic acids (HAAs), …

A Habitat Analysis Of Estuarine Fishes And Invertebrates, With Observations On The Effects Of Habitat-Factor Resolution, Brianna Michaud

USF Tampa Graduate Theses and Dissertations

Between 1988 and 2014, otter trawls, seine nets, and plankton nets were deployed along the salinity gradients of 18 estuaries by the University of South Florida and the Florida Fish and Wildlife Research Institute (FWRI, a research branch of the Florida Fish and Wildlife Conservation Commission). The purpose of these surveys was to document the responses of aquatic estuarine biota to variation in the quantity and quality of freshwater inflows that were being managed by the Southwest Florida Water Management District (SWFWMD).

In the present analyses, four community types collected by these gears were compared with a diversity of habitat …

Comparing Game Simulation To Concept Models For Student-Centered Learning In Biology, Margaurete Romero

USF Tampa Graduate Theses and Dissertations

Science education research continues to demonstrate improved learning with active-learning techniques compared to lectures. However, the question of which active-learning methods are the most effective for learning complex scientific principles in various context still remains. Models are commonly used in activities that allow students to simplify complex systems and understand how components interact. I investigated the outcomes for student learning and engagement of two model-based activities - concept models and game simulations. The activities were conducted in an introductory biology course in sixteen discussion sections. Eight sections were assigned to the concept model activity and eight to the simulation activity. …

Corrosion Characteristics Of Magnesium Under Varying Surface Roughness Conditions, Yahya Efe Yayoglu

USF Tampa Graduate Theses and Dissertations

The biggest challenge with magnesium alloy biodegradable implants is the rapid corrosion at the earlier stages of the healing process after implantation. In this research, the impact of surface roughness generated by different means on the corrosion rate of AZ31 magnesium alloy in a simulated biologic environment is investigated. In order to perform accurate experimentation, an in vitro setup is assembled that simulates the human body environment accurately has been prepared using Schinhammer’s in vitro immersion testing setup and Kokubo’s Simulated Body Fluid (SBF). For the immersion test of Mg in SBF, several surface texture groups of Mg have been …

The Self-Directed Learning Actions Of Women Regarding The Menopause Transition, Jamie Johnson Cooper

USF Tampa Graduate Theses and Dissertations

Research indicates that increased knowledge about the menopause transition positively impacts a woman’s attitude about this stage in her life, and a more positive attitude leads to less distress during the transition. However, there has been no research regarding how women gain this knowledge about menopause, what factors in her environment may hinder or assist her, or how women’s knowledge of menopause is leveraged by health care providers to help facilitate her menopause transition.

The purpose of this research was to explore the self-directed learning actions of women regarding their search for information about menopause, and to understand what factors, …

Resource Use Overlap In A Native Grouper And Invasive Lionfish, Joseph Schmidt Curtis

USF Tampa Graduate Theses and Dissertations

Invasive species can severely disrupt biological communities through their interactions with native organisms, yet little is known about the response of marine predators to the establishment of a competitive invasive fish. In the western Atlantic, invasive Indo-Pacific lionfishes (Pterois spp.) may represent a novel competitor to several commercially and ecologically important native species. However, there is a scarcity of empirical research documenting comparative resource use of cohabitant lionfish and native fishes, as well the physiological consequences that may result from interspecific interactions with the invasive species. For this thesis, I conducted two studies designed to elucidate the strength …

Using Auditory Feedback To Improve Striking For Mixed Martial Artists, Frank Krukauskas Krukauskas

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to evaluate, auditory feedback as a training procedure to increase the effectiveness of throwing a "right cross.” Auditory feedback was evaluated in multiple baselines across behaviors design with 4 mixed martial arts students, two males and two females, 25-54 years old. The percentage of correct steps of the right crosses.” was stable .during baseline for all participants improved substantially following the introduction of the auditory feedback, and maintained at 90 percent or more for all participants during follow-up.