Digital Commons Network^™

A Novel Abi-Domain Protein Controls Virulence Determinant Production In Staphylococcus Aureus, Stephanie Michelle Marroquin

USF Tampa Graduate Theses and Dissertations

A major factor in the success of Staphylococcus aureus as a pathogen is its vast arsenal of virulence determinants and, more importantly, the tight and precisely- timed regulation of these factors. Here we investigate the product of the S. aureus gene, SAUSA300_1984, encoding a putative transmembrane protein. This as yet uncharacterized protein belongs to the Abi (abortive infection) family, which are commonly annotated as CAAX-proteases, and are significantly understudied in prokaryotes. In S. aureus the disruption of SAUSA300_1984 results in a drastic reduction of proteolytic and hemolytic activity, as well as diminished pigmentation. This phenotype appears to be mediated through …

Improving Dementia Care Through Online Training Programs: A Systematic Review And Evaluation, Michelle L. Pleasant

USF Tampa Graduate Theses and Dissertations

Over the next thirty years, Alzheimer’s disease rates will increase alongside global aging. To handle the anticipated increase in demand, knowledgeable and skilled dementia caregivers are in need throughout the long-term care spectrum. Online training programs have emerged as a viable and convenient platform to educate both formal and informal caregivers. The first and second study systematically reviewed online dementia training programs and evaluated the CARES® Dementia Basics Training Program among formal and informal caregivers.

The first study is a systematic review of online dementia-based training programs for both formal and informal caregivers conducted using the Preferred Reporting Items for …

Simulation Of Hemiparetic Function Using A Knee Orthosis With Variable Impedance And A Proprioception Interference Apparatus, Christina-Anne Kathleen Lahiff

USF Tampa Graduate Theses and Dissertations

Individuals with stroke have neuromuscular weakness or paralysis on one side of the body caused by some muscles disengaging and overexciting other muscles. Hyperextension of the knee joint and complete lack of plantar flexion of the ankle joint are common symptoms of stroke. This thesis encompasses the simulation of hemiparetic function using both a knee orthosis with variable impedance, specifically in terms of stiffness and damping, and the Proprioception Interference Apparatus.

The section regarding the knee orthosis with variable impedance focuses on the creation and implementation of a small, lightweight, and adjustable orthotic device to be positioned around the knee …

Opportunities To Mainstream Gender In Water And Wastewater Infrastructure Projects: A Case Study In Barbados, Wainella Isaacs

USF Tampa Graduate Theses and Dissertations

According to the World Resources Institute, Barbados is one of seven Caribbean countries ranked as being the most water stressed territories in the world. Prevailing drought conditions for the period 2010-2016 further compromised its water security while confirming predictions of a drier regional Caribbean climate. The simultaneous failing of at least 50-year-old water infrastructure at many points in the distribution network adds to these water stress conditions, and presents a financial burden to the local water utility in the form of lost revenues, and increased energy consumption for pumping.

Climate change and its impacts are not gender-neutral, and water infrastructure …

Evaluating A Graduate Professional Development Program For Informal Science Educators, Jeremy Paul Lake

USF Tampa Graduate Theses and Dissertations

This study is an examination and evaluation of the outcomes of a series of courses that I helped build to create a graduate certificate. Specifically, I wanted to evaluate whether or not the online iteration of the Informal Science Institutions Environmental Education Graduate Certificate Program truly provided the long term professional development needed to enhance the skills of the formal and informal educators participating so that they could contribute meaningfully to the improvement of science literacy in their respective communities.

My role as an internal evaluator provided an extraordinary opportunity to know the intent of the learning opportunities and why …

Consistency Of Structural Color Across Molts: The Effects Of Environmental Conditions And Stress On Feather Ultraviolet Reflectance, Rebecca Lynn Windsor

USF Tampa Graduate Theses and Dissertations

Across avian systems, plumage ornamentation is often considered a signal of individual fitness, condition, sex, or status, and varies due to genetics or environmental sources. In species with structural coloration, plumage variation results from differences in the amount of energy allocated to feather growth during molt, presenting a unique opportunity to study the link between individual quality and ornamentation. In cooperative breeding species, such as the Florida Scrub-Jay (Aphelocoma coerulescens), the role of structural color as a signal is particularly important because helpers may delay breeding for one to several years and competition for space is high. Florida Scrub-Jay juveniles …

Geographical Information Systems (Gis) Applied To Urban Nutrient Management: Data Scarce Case Studies From Belize And Florida, Charlotte Juliane Haberstroh

USF Tampa Graduate Theses and Dissertations

Nutrient inputs into the environment greatly impact urban ecosystems. Appropriate management strategies are needed to limit eutrophication of surface water bodies and contamination of groundwater. In many existing urban environments, retrofits or complete upgrades are needed for stormwater and/or wastewater infrastructure to manage nutrients. However, sustainable urban nutrient management requires comprehensive baseline data that is often not available. A Framework for Urban Nutrient (FUN) Management for Geographic Information Systems (GIS) was developed to specifically address those areas with limited data access. Using spatial analysis in GIS, it links water quality, land use, and socio-demographics, thereby reducing data collection and field-based …

An Exploration Of The Relationship Between Child Welfare Workers’ Ambivalent Sexism And Beliefs About Father Involvement, Katrina Lee Brewsaugh

USF Tampa Graduate Theses and Dissertations

Research over the last few decades has consistently found that fathers are not routinely included in the provision of child welfare services. The current study examined whether ambivalent sexism on the part of child welfare workers was related to their beliefs about involving fathers. Ambivalent sexism theory posits that gender stereotypes include subjectively positive beliefs in addition to hostile beliefs that both serve to perpetuate patriarchal systems. Participants (N = 490) were currently front-line child welfare workers in the United States who completed an online survey assessing ambivalent sexism and beliefs about father involvement in child welfare cases. Ambivalent sexism …

Social Supports, Stress And Birth Outcomes Among Latina Mothers In Pinellas County, Florida, Maridelys Detres

USF Tampa Graduate Theses and Dissertations

Social supports are linked in public health research to improved birth outcomes. This study explored the relationship of social supports, stress and birth outcomes among pregnant Latinas in Pinellas County, Florida. A sample of 411 Healthy Start women at risk of poor birth outcomes participated in this study (99 Latinas, 142 Black, and 158 White). Study methods included ANOVA, Principal Component Analysis, multivariable regression, logistic regression, and structural equation modeling to identify significant associations between social support scores, stress scores, demographics and health risk factors with infant birth weight, preterm and small for gestational age by ethnic group. Study findings …

Recombinant Elastin Based Nanoparticles For Targeted Gene Therapy, Dagmara Anne Monfort

USF Tampa Graduate Theses and Dissertations

Gene therapy is a technique used to inactivate, replace or insert a corrective copy of a defective gene in order to help diseased tissues to function properly. Gene therapy is a promising treatment for many diseases cancer, cystic fibrosis, and Parkinson’s. There are different methods to introduce a gene to the cell; one of them is the use of viruses. Among viruses, lentiviruses have been popular vectors for gene delivery due to their efficient mode of gene delivery. However, the non-specific delivery of genes associated with viruses may result in undesirable side effects. Here, we propose a heterogeneous nanoparticle delivery …

Land Use, Stream Stability, And Benthic Invertebrates In A Dry Forest Watershed Of Western Costa Rica, Jacqueline Ann Demko

USF Tampa Graduate Theses and Dissertations

There is a paucity of data on dry forests, the most threatened biome in the tropics. The Nandamojo is a tropical dry forest watershed in Western Costa Rica that has been impacted by varying degrees of human induced modifications. This research was conducted to examine the influence of land use and channel characteristics on invertebrate communities within a sub basin of the Nandamojo watershed. This study addressed three hypotheses: (1) sites with low tree cover and small riparian buffer zones will have high erosion, (2) macroinvertebrate abundance will be lower at sites with low channel stability values, and (3) benthic …

Science In The Sun: How Science Is Performed As A Spatial Practice, Natalie Kass

USF Tampa Graduate Theses and Dissertations

This study analyzes how spatial organization impacts science communication at the St. Petersburg Science Festival in Florida. Through map analysis, qualitative interviews, and a close reading of evaluation reports, the author determines that sponsorship, logistics, exhibitor ambience, and map usability and design are the factors most affecting the spatial performance of science. To mitigate their effects, technical communicators can identify these factors and provide the necessary revisions when considering how science is communicated to the public.

Neighborhood Deprivation, Food Insecurity And Gestational Weight Gain, Sabrina Luke

USF Tampa Graduate Theses and Dissertations

Gestational weight gain outside the recommended ranges puts women at risk for pregnancy complications and adverse birth outcomes. Food insecurity and environmental factors including neighborhood deprivation may influence gestational weight gain. This research 1) examines the impact of neighborhood deprivation on gestational weight gain, 2) identifies if the association varies by selected maternal characteristics, 3) examines the relationship between food insecurity and gestational weight gain, 4) determines if stress mediates the relationship between food insecurity and gestational weight gain, and 5) examines whether selected maternal characteristics mediate this relationship. The research was conducted through the analysis of the Pregnancy Risk …

Hydroalkynylation Of Oxocarbenium Intermediates Via Au(I) Catalysis, Courtney Smith Smith

USF Tampa Graduate Theses and Dissertations

Au(I) catalysis has recently emerged as a powerful tool for the realization of a broad range of organic transformations. Despite this rapid development, attaining selectivity and maintaining catalyst stability remain significant challenges. Rational ligand design, such as the employment of NHC or TA ligands, has been used to confront these issues. This thesis focuses on the use of Au(I) catalysts bearing these ligands for the selective hydroalkynylation of enol ethers. By employing a TA-Au stabilized catalyst, [(OAr)₃PAu(TA-H)]OTf, the intermolecular hydroalkynylation of enol ethers, a substrate that is well-known to promote decomposition of the gold cation, was efficiently achieved. …

What Makes Water Policy Sustainable? An Analysis Of Water Policy In Us Cities, Rebecca Brady David

USF Tampa Graduate Theses and Dissertations

This dissertation works to create a clearer understanding of sustainability in water policy. Current water policy in four US cities was compared to a matrix of recommended sustainability themes that have been presented in the literature to determine the extent of which these themes have been implemented into water policy. To best analyze policy for sustainability it is necessary to look at the policy of cities that are considered sustainable. This was determined by a city’s inclusion in “Most Sustainable US Cities” lists. The two cities that best represented sustainability were Austin, TX and San Francisco, CA. The research also …

An Examination Of John Burton’S Method Of Conflict Resolution And Its Applicability To The Israeli-Palestinian Conflict, John Kenneth Steinmeyer

USF Tampa Graduate Theses and Dissertations

This paper argues that the interactive problem-solving workshops created by political scientist John Burton and applied to the Israeli-Palestinian conflict by social psychologist Herbert Kelman, while not, as yet, resulting in a just and permanent peace agreement, are effective in resolving intractable conflict, and, if persistently used, can significantly help to produce such an agreement. This is done by closely examining two books of Burton and a series of articles by Kelman to describe their process; the characteristics of intractable conflict are also reviewed from the work of social psychologist Daniel Bar-Tal. It is then argued that the psychological elements …

Impedance Optimized Electric Pulses For Enhancing Cutaneous Gene Electrotransfer, Reginald Morley Atkins

USF Tampa Graduate Theses and Dissertations

Electric field mediated gene delivery modalities have preferable safety profiles with the ability to rapidly transfect cells in vitro and in vivo with high efficiency. However, the current state of the art has relied on trial and error studies that target the average cell within a population present in treated tissue to derive electric pulse parameters. This results in fixed gene electrotransfer (GET) parameters that are not universally optimum. Slow progress towards the validation of a mechanism that explains this phenomena has also hindered its advancement in the clinic. To date, GET methods utilizing feedback control as a means to …

Mechanisms Of Ikbke Activation In Cancer, Sridevi Challa

USF Tampa Graduate Theses and Dissertations

Cancer is the second leading cause of death in the USA and it is expected to surpass heart diseases making it important to understand the underlying mechanisms of cancer. The efforts to target single signaling molecule showed little success in increasing the patient survival and it can be due to increased compensation for cell survival by alternative pathway activations. Hence comprehensive understanding of the alternative signaling pathways may help us treat cancer better. Chronic inflammation is attributed to increased risk of cancer and emerging studies show the growing importance of both canonical and non-canonical IκB kinases such as IKKα, IKKβ, …

Changing The Pathobiological Paradigm In Myelodysplastic Syndromes: The Nlrp3 Inflammasome Drives The Mds Phenotype, Ashley Basiorka

USF Tampa Graduate Theses and Dissertations

Note: Portions of this abstract have been previously published in the journal Blood, Basiorka et al. Blood. 2016 Oct 13, and has been reproduced in this manuscript with permission from the publisher.

Myelodysplastic syndromes (MDS) are genetically diverse hematopoietic stem cell malignancies that share a common phenotype of cytological dysplasia, ineffective hematopoiesis and aberrant myeloid lineage maturation. Apoptotic cell death potentiated by inflammatory cytokines has been considered a fundamental feature of MDS for over two decades. However, this non-inflammatory form of cell death cannot account for the inflammatory nature of these disorders. We report that a hallmark of lower-risk …

Lilliput Effect Dynamics Across The Cretaceous-Paleogene Mass Extinction: Approaches, Prevalence, And Mechanisms, Matthew Brett Jarrett

USF Tampa Graduate Theses and Dissertations

An organism's body size entails both physiological and ecological costs. Furthermore, as a parameter in analyzing organisms, it represents a fundamental and essential morphometric character. Reductions in size following mass extinction is a commonly observed phenomenon in the fossil record. This study examines the evolutionary significance of this phenomenon termed the: 'Lilliput Effect' by proposing that it represents a rapid evolutionary response to altered selection pressures during a mass extinction. This primary hypothesis is evaluated against two additional hypotheses of size reduction: 1) stunted growth as a response to stressed ecosystems, and/or 2) mass extinctions are size selective.

These hypotheses …

The Apatow Aesthetic: Exploring New Temporalities Of Human Development In 21st Century Network Society, Michael D. Rosen

USF Tampa Graduate Theses and Dissertations

This thesis offers a critical examination of what I call the “Apatow aesthetic” in order to analyze the social processes of growing up in contemporary neoliberal network society. While doctors, psychologists and social scientists still proffer a model of mid- 20th century human development centered around a chronologically-determined life cycle, the Apatow aesthetic imagines a non-linear reality where traditional life events and social practices don’t always correspond to specific age groups. Specifically, I argue, the Apatow aesthetic subjects the spectator to the pleasures and pains of these life-cycle disruptions, and reveals the unfolding of a new cultural shift which challenges …

Mitochondrial Heteroplasmy Contributes To The Dynamic Atovaquone Resistance Response In Plasmodium Falciparum, Sasha Victoria Siegel

USF Tampa Graduate Theses and Dissertations

Of the considerable challenges researchers face in the control and elimination of malaria, the development of antimalarial drug resistance in parasite populations remains a significant hurdle to progress worldwide. Atovaquone is used in combination with proguanil (Malarone) as an antimalarial treatment in uncomplicated malaria, but is rendered ineffective by the rapid development of atovaquone resistance during treatment. Previous studies have established that de novo mutant parasites confer resistance to atovaquone with a substitution in amino acid 268 in the cytochrome b gene encoded by the parasite mitochondrial genome, yet much is still unknown about how this resistance develops, and whether …

The Effects Of Phytohormones And Isoprenoids In Dihydroartemisinin-Induced Dormancy In The Erythrocytic Stages Of Plasmodium Falciparum, Marvin Duvalsaint Duvalsaint

USF Tampa Graduate Theses and Dissertations

Our ability to control malaria has been challenged by increasing antimalarial resistance. Plasmodium falciparum undergoes dormancy in the blood stages which is hypothesized to be a means by which they are able to survive under drug pressure. This helps select for resistant parasites which grow following removal of drug. The mechanisms behind dormancy and the subsequent recrudescence are not fully understood but translating knowledge from related organisms which undergo a similar phenomenon might shed some light. Higher plants utilize dormancy during the early development stages to survive under unfavorable conditions, increasing fitness of the seedling and ensuring viability when this …

A Longitudinal Examination Of A Ssi-Embedded Experiential Environmental Education Course And Environmental Behaviors, Mark H. Newton

USF Tampa Graduate Theses and Dissertations

A perennial goal of environmental education is to produce a scientifically literate citizenry capable of negotiating and resulting complex environmental problems. Popular methods of environmental education instruction tend to overemphasize scientific content knowledge and neglect to consider ethical and moral aspects of the problem. This qualitative study examines the longitudinal association between an experiential environmental education course infused with SSI instruction and students’ environmental behaviors. The results indicate that several students’ conceptualizations of contentious environmental issues change after completing the course and specifically. Furthermore, students’ willingness to act to resolve contentious environmental issues was most closely associated with their environmental …

Vdr-Ripk1 Interaction And Its Implications In Cell Death And Cancer Intervention, Waise Quarni

USF Tampa Graduate Theses and Dissertations

Receptor interacting protein kinase 1 (RIPK1) is an enzyme acting downstream of tumor necrosis factor alpha to control cell survival and death. RIPK1 expression has been reported to cause drug resistance in cancer cells; but so far, no published studies have investigated the role of RIPK1 in vitamin D action. In the present study, we investigated whether RIPK1 played any role in 1,25-dihydroxyvitamin D3 (1,25D3)-induced growth suppression. In our studies, RIPK1 decreased the transcriptional activity of vitamin D receptor (VDR) in luciferase reporter assays independently of its kinase activity, suggesting a negative role of RIPK1 in 1,25D3 action. RIPK1 also …

Reification, Resistance, And Transformation? The Impact Of Migration And Demographics On Linguistic, Racial, And Ethnic Identity And Equity In Educational Systems: An Applied Approach, Rebecca Ann Campbell

USF Tampa Graduate Theses and Dissertations

Using an applied anthropological approach focused on language, this study investigates the relationship between linguistic, racial, and ethnic identities and school resource access in the context of migration. This project examines how these identities are established, experienced, reified, and resisted by various school actors. Exposing power at its roots through a multi-level analysis, this research informs on how people negotiate socialization into particular identities, propelling them toward positions in school and society of varying opportunity.

Focused on two elementary schools in a central Florida county that has been and is undergoing demographic changes, this work offers applications for educational institutions …

Gene Expression Profiling And The Role Of Hsf1 In Ovarian Cancer In 3d Spheroid Models, Trillitye Paullin

USF Tampa Graduate Theses and Dissertations

Ovarian cancer is the most lethal gynecological cancer, with over 200,000 women diagnosed each year and over half of those cases leading to death. These poor statistics are related to a lack of early symptoms and inadequate screening techniques. This results in the cancer going undetected until later stages when the tumor has metastasized through a process that requires the epithelial to mesenchymal transition (EMT). In lieu of traditional monolayer cell culture, EMT and cancer progression in general is best characterized through the use of 3D spheroid models. In this study, we examine gene expression changes through microarray analysis in …

Confinement Effects And Magnetic Interactions In Magnetic Nanostructures, Kristen Lee Stojak Repa

USF Tampa Graduate Theses and Dissertations

Multifunctional nanocomposites are promising for a variety of applications ranging from microwave devices to biomedicine. High demand exists for magnetically tunable nanocomposite materials. My thesis focuses on synthesis and characterization of novel nanomaterials such as polymer nanocomposites (PNCs) and multi-walled carbon nanotubes (MWCNTs) with magnetic nanoparticle (NP) fillers.

Magnetite (Fe₃O₄) and cobalt ferrite (CoFe2O4) NPs with controlled shape, size, and crystallinity were successfully synthesized and used as PNC fillers in a commercial polymer provided by the Rogers Corporation and poly(vinylidene fluoride). Magnetic and microwave experiments were conducted under frequencies of 1-6 GHz in the presence of …

Quantitative Proteomic Investigation Of Disease Models Of Type 2 Diabetes, Mark Gabriel Athanason

USF Tampa Graduate Theses and Dissertations

PANcreatic DERived factor (PANDER, FAM3B) is a member of a superfamily of FAM3 proteins that are uniquely structured and strongly expressed from the endocrine pancreas and co-secreted with insulin. Unique animal models available to our lab have indicated that PANDER can induce a selective hepatic insulin resistant (SHIR) phenotype whereby insulin signaling is blunted yet lipogenesis is increased. The complexity of the biological networks involved with this process warranted the logical approach of employing quantitative mass spectrometry based proteomic analysis using stable isotope labeling of amino acids in cell culture (SILAC) to identify the global proteome differences between the PANDER …

The Effects Of Synthetic And Dietary Therapeutics On Learning, Memory, Motor Coordination, And Seizure In An Angelman Syndrome Mouse Model, Stephanie Lynn Ciarlone

USF Tampa Graduate Theses and Dissertations

Angelman syndrome (AS) is a rare genetic and neurological disorder presenting with severe developmental delay, ataxia, epilepsy, and lack of speech. AS is associated with a neuron-specific loss of function of the maternal UBE3A allele, a gene encoding an E3 ubiquitin ligase. Currently, no cure exists for this disorder; however, recent research using an AS mouse model suggests that pharmacological intervention is plausible, and can alleviate some of the detrimental phenotypes reported in AS patients.

Although there is no curative treatment for AS, seizure medication and behavioral therapies are most commonly prescribed in order to minimize symptoms. However, these options …