Digital Commons Network^™

Rapid Vertical Flow Assay On Aunp Plasmonic Paper For Sers-Based Point Of Need Diagnostics, Richard Frimpong

Theses and Dissertations

SERS based immunoassays for point-of-care diagnostics is a promising tool to facilitate biomarker detection for early disease diagnosis and control. The technique is based on a sandwiched system in which antigen is first captured by a selective substrate and then labeled by an extrinsic Raman label (ERL). Here, we report on the use of gold nanoparticle modified filter paper as a novel capture membrane in a vertical flow format. This vertical flow configuration affords reproducible flow of sample and label through the capture substrate to overcome diffusion limited kinetics and significantly reduced assay time. The filter paper was selected due …

Adsorption Behavior Of Chemically / Charged Modified Antibodies On Gold Nanoparticles, Samuel Okyem

Theses and Dissertations

Gold nanoparticles (AuNPs) have been exploited in the various domains of science such as drug delivery, bio-sensing, immunoassays and environmental sensors, due to their optical properties and intriguing surface chemistry. Different scientific procedures have been used to effectively immobilize antibodies onto AuNPs. Although acceptable outcomes have been achieved in the immobilization of antibodies onto AuNPs, the sensitivity of these immobilized antibodies to target antigen or binding sites is limited due to improper orientation of the antibodies. Also, the possibility of nanoparticle aggregation when exposed to proteins limits its biomedical applicability.

There is some evidence that the surface charge of antibodies …

Investigating The Reversibility Of Protein Adsorption On Gold Nanoparticles And The Role Of Free Thiol, Awosiji Olatunde Awotunde

Theses and Dissertations

Protein-gold nanoparticle (AuNP) bioconjugates have many potential applications in nanomedicine. A thorough understanding of the interaction between the protein and AuNP is critical to engineering these functional bioconjugates with desirable properties. In this work, we investigate the role of free thiols presented by the protein on the stability of the protein-AuNP conjugate. Human serum albumin (HSA) was modified with 2-iminothiolane (Traut’s reagent) to introduce additional thiols on the protein surface, and three variants of HSA were synthesized to present 1, 5, and 20 free thiols by controlling the molar excess of the chemical modifier. Protein exchange studies on AuNPs were …

Sleuths As Social Activists: Negotiations Of Power & Morality In Ya Sleuthing Stories, Heather Leigh Sanford

Theses and Dissertations

In “Sleuths as Social Activists: Negotiations of Power & Morality in YA Sleuthing Stories” I aim to explore how adolescent sleuthing stories intersect with negotiations of power, subjectivity, and relationality within the structures and confines of adolescent literature to challenge oppressive paradigms. This project will begin with a focus on gender dynamics, using ethics of care to compare Wendelin Van Draanen’s girl sleuth Sammy Keyes to Anthony Horowitz’s boy sleuth Alex Rider. This analysis of gender will then expand into an intersectional exploration of how age and race additionally implicate how Sammy Keyes and Alex Rider negotiate power and morality …

Synthesizing Modified Polymeric Nanoparticles For Biofilm Inhibition, Antibiotic Encapsulation, And Specific Targeting Against Pseudomonas Aeruginosa, Logan Schnorbus

Theses and Dissertations

Widespread usage of antibiotics is a growing concern due to antibiotic resistance development in bacteria. This is due to common use of antibiotics in agricultural, livestock, and clinical usage. Antibiotic resistance is developing at a rate in which it is outpacing new drugs on the market. New strategies in drug development are necessary to combat the increasing resistance. We designed several motifs of sugar-modified nanoparticles to inhibit the biofilm formation of Pseudomonas aeruginosa.

P. aeruginosa is an opportunistic pathogenic bacterium that is responsible for common life-threatening infection in hospitals. This gram-negative opportunistic pathogenic bacterium infects hosts with compromised immune systems …

An In Silico Study Of Small Molecule Anti-Cancer Agents Targeting Dna G-Quadruplexes, Holli-Joi Sullivan

Theses and Dissertations

Free ligand binding molecular dynamic simulations are a powerful tool used to probe the ligand binding process, mechanism and pathway and the insight gained can help expedite the early stages of drug discovery. Using these methods, we model the binding of two small molecule anti-cancer agents BRACO19 and CX-5461 to a variety of DNA G-quadruplexes (G4s) and a DNA Duplex. The first study focuses on the binding of BRACO19 to three different topological folds (parallel, anti-parallel and hybrid) of the human telomeric G4s. Our detailed analysis identified the most stable binding modes were end stacking and groove binding for the …

Influence Of Teachers’ Psychological Safety, Principals’ Transformational Leadership, And Organizational Uncertainty On Schools As Learning Organizations, Lloyd D. Jackson

Theses and Dissertations

The primary function of a school is learning. What this causal-comparative and correlational study sought to resolve is whether schools which are designed for learning can learn. The purpose of the study was four-fold. First, to validate the Dimensions of a Learning Organization Questionnaire (DLOQ) as an instrument to be used to within the school context. Secondly, to ascertain if there was a difference in how the factors of the DLOQ loaded in relation to the achievement level of a school. Thirdly, the study was to assess whether the dimensions of learning organization—continuous learning, dialogue and inquiry, collaboration, embedded systems, …

Aces And Substance Use: Understanding The Influence Of Childhood Experiences On Substance Use In Adolescence Across Race And Ethnicity, Tacey Micole Matheson Shurtliff

Theses and Dissertations

Adverse Childhood Experiences (ACEs) affect numerous outcomes in adulthood, but relatively few studies examine their implications for adolescents. Understanding the effects of ACEs is important since adolescent behaviors affect subsequent life course milestones and transitions. One area of the ACEs research that is deficient involves adolescent substance use. In addition, there is a paucity of studies addressing whether the association between ACEs and substance use differs by race/ethnicity. Using data from the Fragile Families and Child Wellbeing Study, this study aims to fill these gaps by (a) examining whether adolescents who experience more ACEs tend to be at higher risk …

Yay Or Nay? A Media Analysis Of How Deception Is Utilized As A Leadership Tactic In The Television Show Sons Of Anarchy, Bruce T. Lanigan

Theses and Dissertations

Deception is a tactic that is seen by individuals on a daily basis. One of the places most people endure deception is in the workplace. This thesis analyzes how leaders utilize deception in the workplace through the television show Sons of Anarchy. Throughout the show, both transformational and transactional leadership styles are present. Various story arcs portraying deceptive actions will be analyzed through the lenses of leader-member exchange theory and interpersonal deception theory. This thesis reveals that transformational leaders utilize deception more effectively, while transactional leaders use deceptive measures more frequently.

Bijels Made By Solvent Transfer Induced Phase Separation: Formation Principles And Transport, Stephen Boakye-Ansah

Theses and Dissertations

Bijels are made of non-equilibrium particle-stabilized emulsions with a bicontinuous arrangement of the constituent fluid phases. They spontaneously form through arrested spinodal decomposition in mixtures of partially miscible liquids and neutrally wetting colloidal particles. Soon after their discovery over 10 years ago, Prof. Mike Cates, Lucasian Professor of Mathematics, predicted their future use as continuously operated cross-flow reactors for chemical reactions between immiscible reactants.

Towards this goal, work in this thesis focuses on designing bijels via Solvent Transfer Induced Phase Separation (STrIPS) for microfluidic transport applications. Structure-function relationships of STrIPS bijels stabilized by silane functionalized nanoparticles are developed. In-situ surfactant …

Evaluating And Improving The Seu Reliability Of Artificial Neural Networks Implemented In Sram-Based Fpgas With Tmr, Brittany Michelle Wilson

Theses and Dissertations

Artificial neural networks (ANNs) are used in many types of computing applications. Traditionally, ANNs have been implemented in software, executing on CPUs and even GPUs, which capitalize on the parallelizable nature of ANNs. More recently, FPGAs have become a target platform for ANN implementations due to their relatively low cost, low power, and flexibility. Some safety-critical applications could benefit from ANNs, but these applications require a certain level of reliability. SRAM-based FPGAs are sensitive to single-event upsets (SEUs), which can lead to faults and errors in execution. However there are techniques that can mask such SEUs and thereby improve the …

Client Experiences Of A Brief Heart Rate Variability Biofeedback Protocol, Sheilagh Fox

Theses and Dissertations

This study investigated the experiences of clients who completed a brief heart rate variability biofeedback protocol. The purposes of this study were to (1) learn about client experiences of biofeedback because almost no previous research has done so and (2) explore the potential role of common factors in biofeedback. Fifteen clients were interviewed and their data analyzed according to the methods of Consensual Qualitative Research (CQR; Hill, 2012). CQR relies on the use of group consensus to construct representations of participant experiences and categorize themes within the data. The results of the study showed that participants generally experienced the HRVB+ …

Sexual Abuse Prevention For Adolescents With Intellectual And Developmental Disabilities: Parent Perceptions And Program Effectiveness, Katie Lyn Barton

Theses and Dissertations

Individuals with Intellectual and Developmental Disabilities (IDD) are at an increased risk of experiencing sexual abuse. While there are evidence-based prevention programs for typically developing children, research addressing the IDD population is lacking. Research is also lacking in parent's attitudes towards sexual abuse prevention in the IDD population. Study 1 used a mixed-methods design to measure parent's perceptions of a sexual abuse prevention program. A survey method was used to gather information from parents (n=79). Differences of a variety of variables were considered but only those focusing on the nature of disability, communication abilities, and severity of disability were significant. …

Success Off The Field: Academic Strategies Of High-Gpa College Athletes, Ashlynn Erbe

Theses and Dissertations

This study investigated the strategies academically successful college student athletes use to do well in their class work, where academic success was defined as a 3.5 GPA for 12 or more credits for the two semesters preceding the study. Data were transcriptions of individual interviews with five male and five female athletes in seven sports at an NCAA Division I university in the western United States. Independent coders analyzed the data and agreed upon themes related to challenges to academic success and strategies to meet those challenges. Findings add to the literature by detailing self-regulatory habits that academically underprepared athletes …

Information Geometry And Model Reduction In Oscillatory And Networked Systems, Benjamin Lane Francis

Theses and Dissertations

In this dissertation, I consider the problem of model reduction in both oscillatory and networked systems. Previously, the Manifold Boundary Approximation Method (MBAM) has been demonstrated as a data-driven tool for reducing the parametric complexity of so-called sloppy models. To be effective, MBAM requires the model manifold to have low curvature. I show that oscillatory models are characterized by model manifolds with high curvature in one or more directions. I propose methods for transforming the model manifolds of these models into ones with low curvature and demonstrate on a couple of test systems. I demonstrate MBAM as a tool for …

Ems Mutagenesis In Quinoa: Developing A Genetic Resource, Brian James Cox

Theses and Dissertations

Chenopodium quinoa, a South American pseudocereal, has valuable agricultural traits such as salt tolerance and drought tolerance, and it has beneficial nutritional properties such as high protein content and a complete amino acid profile. However, problems including disease susceptibility, low harvest index, lodging, seed shattering, low heat tolerance, and saponin content plague quinoa. Genetic resources for quinoa are needed to fix these problems and make quinoa more available throughout the world. We used ethyl methanesulfonate (EMS) to create a mutant population of QQ74 quinoa (USDA GRIN PI 614886) of 5,030 mutant families. We did whole exome sequencing (WES) on 44 …

Creating Library Learning Spaces That Support Twenty-First Century Pedagogy And Student Learning, Deborah Lynn Christoffersen

Theses and Dissertations

University libraries struggle to keep up with rapidly changing technology and the associated change in teaching strategy. Most administrators and librarians are often not trained to assess space needs and struggle to reassign library spaces for non-traditional library use. As such, they often embark on expensive and time-consuming feasibility studies, using (typically) hard-earned monies to complete the research or to pilot a new space. What academic research library administrators and staff lack is an analysis tool for discovering and planning needed renovations and improvements in aging library facilities. The purpose of this research project was to determine how students use …

Pedagogical Approaches And Instructional Content That Predict Increased Acceptance Of Biological Evolution In University Students, Clinton Thomas Laidlaw

Theses and Dissertations

Evolution is the central organizing theory of biology. Without evolutionary theory, biology becomes a somewhat tangential assemblage of facts about living organisms, which is precisely how it is viewed by many students. Many teachers teach evolution in a limited capacity or avoid it entirely due to fear of opposition, lack of confidence in their own understanding, or lack of acceptance of the theory themselves. When evolution is not taught, or is not accepted, it cannot be utilized to make sense of the field, and is quickly forgotten by students. While some studies have shown a correlation between instruction about evolution …

Entropy's Child, Brandon Reed Boulton

Theses and Dissertations

Art, process, and materials offer me an escape from the sometimes-crushing realities of my personal struggle with chronic mental illness. Escape is often my primary motivator for making art. However, personal meaning and understanding sometimes come while I'm working in the studio. Sometimes the meanings of the work only become clear years later. Personal experience and experimenting with materials and processes have led me to an appreciation and awareness of entropy--the second law of thermodynamics. My sculptures use spontaneity, abstraction, and process to dialogue with entropy.

A Corpus-Based Study Of The Gender Assignment Of Nominal Anglicisms In Brazilian Portuguese, Taryn Marie Skahill

Theses and Dissertations

The purpose of this study is to analyze the variability of gender assignment to nominal anglicisms in Brazilian Portuguese and to identify how the orthography of English loanwords and their establishment in the language influences such variation. This study also seeks to identify the most important factors that govern such gender assignment. The data were gathered from two Portuguese corpora, one consisting of more formal and edited language (Corpus do Português, News on the Web) and the other consisting of less formal and unaltered language, such as blog posts (Corpus do Português, Web/Dialects). Forty anglicisms were analyzed in order to …

Study And Characterization Of Conductive Elastomers For Biomedical Applications, Hadis Gharacheh

Theses and Dissertations

Health issues have always been one of humankind's biggest challenges. Over the last century, there has been significant and monumental progress in health and biomedical science, with the end goal of alleviating and eliminating illnesses and ailments. For developing biomedical devices, polymers and elastomers group among other types of biomaterials have been highlighted to be used due to high flexibility, stability, biocompatibility, and mechanical and rheological characteristics. In this work, the characterization of acrylated poly glycerol_sebacate (PGSA) polymer conjugated with bio ionic liquid (BIL) was investigated. Results showed high biocompatibility, high printability with tunable mechanical, adhesive, and conductivity properties. For …

The Effect Of Ethnic Identity On Motivation To Bephysically Active In Schools In Hawai’I, Nathan A. K. Kahaiali'i

Theses and Dissertations

This cross-sectional study examines if there is a relationship between student ethnic identity and their motivation towards physical activity (PA), with a particular interest with students who self-identify as Hawaiian. Results indicate that there is a small but positive correlation between motivational indices and measures of ethnic identity. This study surveyed 301 sixth, seventh, and eighth grade students from one of the Hawaiian Islands. The survey consisted of the Situational Intrinsic Motivation Scale (SIMS) instrument (Guay, Vallerand, & Blanchard, 2000) to measure motivation of PA and the Multigroup Ethnic Identity Measure-Revised (MEIM-R) instrument (Phinney & Ong, 2007) to measure ethnic …

Landscape Performance Of Annual Bedding Plants Following Foliar Application Of Silicon And Titanium, Vania Maria Pereira

Theses and Dissertations

The U.S. floriculture industry was estimated at $4.77 billion in 2018 with annual bedding plants representing the main segment valued at $1.46 billion dollars. Silicon (Si) and titanium (Ti) are beneficial nutrients which can improve growth and quality of greenhouse horticultural crops when applied exogenously. Information is lacking as to whether Si and Ti enhancement during greenhouse production increases bedding plant performance in the outdoor landscape. Therefore, this study examined landscape performance of four annual bedding plant species treated with foliar applications of Si and Ti during greenhouse production over two growing seasons. Plant species were selected based on U.S. …

Oxadiazinones As Chiral Auxiliaries: Chiral Templates For Asymmetric Conjugate Addition In The Synthesis Of ( R )-( + )-Tolterodine, Fatima Olayemi Obe

Theses and Dissertations

The stereoselective formation of carbon-carbon bonds through the process of conjugate addition has proven to be a very important methodology in synthetic organic chemistry. In this context, oxadiazinones are chiral auxiliaries that have been applied in the asymmetric aldol addition reaction to synthesize valuable synthetic fragments such as the aldol side chain of the multi-drug resistance medicinal agent, hapalosin. This thesis describes efforts that were directed towards employing oxadiazinones as chiral scaffolds for the process of asymmetric conjugate addition with the ultimate objective of using this methodology in the preparation of medicinal agents such as Tolterodine. Preliminary efforts focused on …

Improving The Piezoelectric Properties Of Flexible Polymer-Nanoparticle Energy Harvesters, Muhammet Emin Cavusoglu

Theses and Dissertations

In this research, one of the widely used polymers, poly(vinylidene fluoride)(PVDF), was used to develop thin-film polymer energy harvesters. Dimethylformamide (DMF) and methyl ethyl ketone (MEK) were used to dissolve the PVDF powder. Four different (0%, 3%, 5%, and 7%) ZnO nanoparticle (NP) concentrations were used to enhance the electrical output of the thin-film energy harvesters. A sonication bath and additional MEK were used in dispersing the ZnO NPs to obtained uniform PVDF/ZnO NP solution. The electrode poling technique was used for dipole alignment to improve the electrical performance of the devices. The fabricated samples were tested using the tensile …

Post-Birth Marriage, White-Hispanic Families, And Child Academic Achievement, Sadie Andrews Slighting

Theses and Dissertations

Over the past decade, policymakers have promoted marriage as a pathway to improve child outcomes in single-parent households. However, previous research on single mothers who later married in the United States has failed to examine how the structural advantages and disadvantages of race influence post-birth marriages and the advantage they may confer. I investigate how white advantage—the human- and social-capital benefits that come from being a white individual—acts as a resource distributed differently across three couple configurations. I predict that having access to white advantage via a white parent will improve child academic achievement. Using the US Early Childhood Longitudinal …

Baby On The Way, Sex Gone Away? A Dyadic Investigation Of Sexual Satisfaction In Pregnancy, David Brent Allsop

Theses and Dissertations

Research indicates that having high sexual satisfaction during pregnancy is difficult for couples. This negative trend is important considering that low sexual satisfaction can negatively affect both relationship satisfaction, psychological health, and child outcomes. However, there is evidence that this trend does not apply to all and different groups of couples exist in terms of their sexual satisfaction in pregnancy. Guided by family systems theory and the biopsychosocial model, the current study explored if different subsets of couples, composed of pregnant wives and husbands, exist in terms of their sexual satisfaction during pregnancy and what factors distinguish potential subsets. Using …

Developmental Predictors Of Adolescent Alcohol Intake: A Nonhuman Primate Model, Elizabeth Katherine Wood

Theses and Dissertations

Alcohol abuse is one of the costliest human health problems in the United States. Studies assessing the etiology of alcohol use disorders (AUDs) in adulthood suggest that these disorders are predicted by trait-like differences, such as low or impaired central serotonin or temperamental anxiety. Few studies, however, have assessed neonatal, infant, and adolescent characteristics that lead to alcohol abuse in adolescence. Given that the expression of AUDs is rooted in biological processes, the set of studies presented in this work investigate the early origins of excessive alcohol use in adolescence, with an overall goal of identifying risk factors that may …

Microfabricated Fluidic Devices For Biological Assays And Bioelectronics, Anna V. Bickham

Theses and Dissertations

Microfluidics miniaturizes many benchtop processes and provides advantages of low cost, reduced reagent usage, process integration, and faster analyses. Microfluidic devices have been fabricated from a wide variety of materials and methods for many applications. This dissertation describes four such examples, each employing different features and fabrication methods or materials in order to achieve their respective goals. In the first example of microfluidic applications in this dissertation, thermoplastics are hot embossed to form t-shaped channels for microchip electrophoresis. These devices are used to separate six preterm birth (PTB) biomarkers and establish a limit of detection for each. The next chapter …

A Patient-Specific Adaptation Of The Living Human Heart Model In Application To Pulmonary Hypertension, Yousof Abdel-Raouf

Theses and Dissertations

The Living Heart Project aims to offer medical practitioners and researchers a full-heart electromechanical computational platform to explore and assess clinical cases pertaining to the left ventricle (LV), and the less addressed right ventricle (RV). It does not, however, provide an easy solution to applying this platform to patient-specific cases that account for a large variability among cases. We, therefore, present a solution to modify the Living Human Heart Model (LHHM) to obtain a patient-specific geometry using the thermal expansion method, with iteratively adjusted parameters that accurately simulate the case of a 72-year-old female patient suffering from secondary pulmonary hypertension …