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Cis-Resveratrol Upregulates Tyrosyl-Trna Synthetase And Inhibits The Proliferation Of Select Breast Cancer Cell Lines, Marion Cone Hope Iii

Theses and Dissertations

Breast cancer is one of the most commonly occurring cancers in women. 70% of breast cancer patients express ERα and are treated with tamoxifen (Tam), a drug that is used to directly target ERα. However, 20-30% of cancer patients develop a resistance to Tam. This resistance leads to a worse prognosis and other treatments such as DNA damaging drugs or radiation to eliminate these cells. Resveratrol (RSV) is a polyphenolic- compound found in plants such as grapes and hellebore and is known to evoke anti-cancer effects. While natural resveratrol exists as a mixture of both cis- and trans- isomers, so …

Mechanistic Insight On Vibrio Gazogenes Mediated Intervention Of Aflatoxin Biosynthesis In Aspergillus Flavus, Rubaiya Jesmin

Theses and Dissertations

Aflatoxin is a liver carcinogen, mycotoxin, and a secondary metabolite that is produced by some plant pathogens within the genus Aspergillus when they infect crops such as corn, peanuts, and cotton. Given the significant adverse health and economic impacts of aflatoxin and a predicted rise of crop contamination with mycotoxins caused by a changing climate, there is a pressing need for exploring new aflatoxin mitigation methods. Previous studies in our laboratory have shown that metabolites from Vibrio gazogenes, an estuarine non-pathogenic bacterium, can inhibit aflatoxin synthesis. However, a direct interaction study between V. gazogenes and aflatoxin producing strains has …

17 Β-Estradiol And Phytoestrogens Attenuate Apoptotic Cell Death In Hiv-1 Tat Exposed Primary Cortical Cultures, Sheila Marie Adams

Theses and Dissertations

Despite the advent of highly active antiretroviral therapy (HAART), neurological disorders develop in most people infected with human immunodeficiency virus type 1 (HIV-1). The poor CNS delivery of anti-retrovirals allows continued virus production and renders neurons susceptible to the toxic effects of released viral proteins. The neurotoxic potential of HIV-1 viral protein Tat has been shown to include excitotoxicity, oxidative stress, and mitochondrial dysfunction leading to subsequent cell death. Thus, Tat is thought to have a key role in the pathogenesis of HIV-associated neurodegeneration. Estrogens are universally considered to be neuroprotective, as such; estrogens have positive implications in the treatment …

Using Genetic Diversity To Differentiate Mosquito Populations, Rachel Christine Malampy

Theses and Dissertations

Experiment 1: Little is known about Aedes aegypti females sharing oviposition sites. We examined four different oviposition sites in McAllen, TX. A mitochondrial gene was used to analyze the variation within the oviposition site to understand how many egg-laying females are sharing oviposition sites. These results will aid in the understanding of Aedes. aegypti oviposition behavior and contribute to more effective mosquito control techniques.

Experiment 2: Mosquito flight patterns and movement from habitat to habitat can drastically influence the spread of diseases. The anthropophilic Aedes aegypti mosquito has historically been identified as having a very limited flight range. Studies also …

21st Century Physics Homework: A Mixed-Methods Approach Evaluating How An Individualized Online Homework Platform Can Provide Quality Feedback And Help Physics Students Engage In Self-Regulated Learning, Scott Philip Buhr

Theses and Dissertations

American secondary students spend many hours each week working on homework. To get the most out of this homework, students need to receive quality feedback and engage in self-regulated learning when completing homework tasks. For teachers, traditional paper-and-pencil homework means extra time spent grading rather than giving good feedback. This study aims to answer the following questions: 1) How and to what extent does the implementation of individualized online homework and feedback impact self-regulated learning among Honors Physics high school students? and 2) How does the implementation of individualized online homework impact students’ perception of the feedback quality provided by …

Function Of Gpr68 In Normal Hematopoiesis And Malignant Hematopoietic Diseases, Xiaofei He

Theses and Dissertations

G protein-coupled receptor 68 (GPR68), as a proton-sensing receptor, is involved in numerous physiological processes. However, its specific functions in normal hematopoiesis and malignant hematopoietic diseases were unknown. Using a genetic loss of function approach (i.e. whole-body Gpr68^-/- mouse model), B lymphocytes were reduced under stressed conditions, such as aging and hematopoietic regeneration. However, hematopoietic stem cells (HSC) from Gpr68^-/- mice exhibited comparable function as HSC from wild type mice either under steady-state or upon stress. To determine whether the unaltered HSC function of Gpr68^-/- mice was due to compensatory effects of Gpr68 in nonhematopoietic cells, a …

Association Between Urinary Enterolignans As A Marker For Gut Microbiome Diversity And Depression In Nhanes, Marie Knoll

Theses and Dissertations

Depression is a chronic disease which inflicts functional and emotional impairment that has been a growing issue in the United States (US). Depression is the most prevalent mental disorder in the US and is the leading cause of disability in the world. By 2030, It is projected that major depressive disorder will be the second largest contributor to global burden of disease. Causes of depression are still largely unknown and treatments are often expensive, time consuming, and only available to certain populations. Treatment often requires a combination of medicines and therapy to be effective. In recent years, the gut microbiome …

Authentic Assessments And Their Impact On Engagement Of Gifted Learners In Washington County Public Schools: An Action Research Study, Felicia Jo Lowman-Sikes

Theses and Dissertations

This mixed-methods, action research project focused on how gifted learners perceived their levels of engagement when presented with differing assessment types. The project, which took place in Washington County Virginia Public Schools, investigated how middle and high school gifted learners’ engagement differed when completing authentic assessments versus multiple choice assessments. A purposeful sample was chosen with participants then completing Likert-type surveys and semi-structured interviews. Classroom observations were conducted at both the middle and high school levels. Data and findings were triangulated to complete the research process.

Temporal Differences In The Emission And Atmospheric Dispersion Of Particulate Matter From Waste And Drinking Water Treatment Facilities, Abdulmalik Alsaif

Theses and Dissertations

Many countries encounter a growing shortage of water, and the reuse of treated or untreated wastewater is their main source. Wastewater Treatment Plants (WWTPs) are mainly designed to remove organic nutrients, large solids, and chemical substances. There are some possible issues regarding WWTPs that are close to residential areas around the world. One of the environmental effects from WWTP that might cause air pollution would be the emission of toxic chemical gases and microorganisms via aerosols that transport bacteria as well as viruses and fungal spores which can be harmful to human health. Therefore, particulate matters (PM) and metrological parameters …

Adhd Symptoms And Alcohol Expectancies: The Moderating Roles Of Parenting And School Climate, Melanie Morse

Theses and Dissertations

Attention-deficit/hyperactivity disorder (ADHD) is a common childhood behavioral disorder that often extends into adulthood and is associated with negative health outcomes, including alcohol use. Alcohol expectancies are a widely studied predictor of alcohol use, and have been shown to predict alcohol use among individuals with ADHD. Positive alcohol expectancies appear to be particularly related to alcohol use as well as ADHD. However, a number of broad social factors including parent, peer, and school/environmental influence likely impact the relation between ADHD and alcohol expectancies. The current study examined positive parenting, parental monitoring, parental involvement, and school climate as moderators of the …

Dialogue Sessions On Diversity, Equity, And Inclusion Topics: A Phenomenological Action Research Study In An Independent School Setting, Erica Forniss Wiltshire

Theses and Dissertations

Administrators and teachers play an integral role in creating educational spaces where all students can feel safe, welcome, and appreciated. Inspired by the unique responsibility of educators and guided by the work of noted proponents of cultural competence, this exploration is approached through a triplicate lens including phenomenological philosophy, critical race theory, and social reconstructionism. This critical social reconstructionist-hermeneutic phenomenological action research study seeks to gain a deeper understanding of early elementary, independent school teachers’ views on cultural competence before and after three targeted dialogue sessions.

Spatiotemporal Dynamics Of Benthic Microalgae In South Carolina Shelf Sediments, Sarah N. Zaunbrecher

Theses and Dissertations

Benthic microalgae (BMA) play essential roles in food webs and regulating nutrient exchange at the sediment-water interface in continental shelf ecosystems. However, shelf BMA are not widely studied due to the difficulties sampling the upper few millimeters of shelf sediments. A few published studies have highlighted the high spatiotemporal distribution of BMA, but detailed explanations for this known variability are limited. The objectives of this study were to quantify BMA biomass variability on scales of cm to km and relate these measurements to in situ nutrient concentrations, sediment grain size, in situ irradiance, depth, and other environmental factors. Water and …

Fundamental Understanding Of Plasma Discharge Formation In Liquid And Multiphase Configurations Through Multiphysics Modeling, Ali Charchi Aghdam

Theses and Dissertations

During the last two decades, non-thermal plasma discharges in and in contact with liquids have received significant attention due to their wide range of applications including chemical analysis, medical, water treatment, fuel processing, etc. Despite the tremendous interest and advances attained in the experimental studies, modeling efforts providing a comprehensive understanding of the underlying physicochemical processes are limited. There is still no unified theory on plasma formation in dense medium and various theories have been proposed such as the presence of bubbles and tunneling which are topics of debate. In the first part of this study, a mathematical model is …

Evaluation Of Temporal Damage Progression In Concrete Structures Affected By Asr Using Data-Driven Methods, Vafa Soltangharaei

Theses and Dissertations

Alkali-silica reaction (ASR) is a chemical reaction, which causes damage in concrete structures such as bridges, dams, and nuclear containments and powerplant structures. The ASR-induced damage may endanger the integrity and serviceability of structures. Several methods such as visual inspection, petrographic analysis, demountable mechanical strain gauges, and cracking index have been utilized for study the effect of ASR on structures, which are not always efficient in early damage detection and some are destructive and prohibited in nuclear structures. Nondestructive methods and structural health monitoring techniques can be alternatives for the condition assessment of structures. Among the nondestructive methods, acoustic emission …

Understanding The Influence Of Changing Climate On Mycotoxin Contamination Of Food And Indoor Fungi-Mediated Respiratory Illness, Mayomi Helen Omebeyinje

Theses and Dissertations

Over the years, climate change has been a major core issue in public and environmental health. The World Health Organization (WHO) estimated that about 250,000 additional death per year would be attributed to climate change between 2030 and 2050 if this situation persists. Since our population depends on (but not limited to) the availability of clean water, good air quality, and exposure to environmental hazards/pathogens, the overall health impact caused by climate change is likely to be overwhelming in the nearest future. This dissertation explored the influence of climate change on fungi diseases and pathogenesis after severe weather conditions (hurricanes …

Hydrogen Peroxide And Antioxidant Enzymes Moderate Interaction Of The Carbon And Oxygen Cycles At The Redoxcline, Fan Wang

Theses and Dissertations

Hydrogen peroxide is a reduced form of dioxygen produced in natural waters from a manifold of abiotic and biological processes. Hydrogen peroxide is highly redox active and it often serves to initiate the formation of reactive oxygen species in the environment. It typically exists in natural waters at concentrations ranging from as low as ~ 1 nanomolar in “blue water” marine environments to as high as 10 micromolar near actively effluxing sediments. It has the potential to cause significant toxicity in aquatic organisms, which have evolved a collection of antioxidant enzymes including peroxidase, catalase, and superoxide dismutase. Work in this …

The Role Of Chromatin Remodeling Protein And Transcriptional Repressor, Ing4, In Hematopoiesis Regulation, Melanie Rodriguez

Theses and Dissertations

The hematopoietic compartment is tasked with the establishment and maintenance of the entire blood program. Key to this process are hematopoietic stem cells (HSCs), which possess the unique ability to self-renew and differentiate to replenish blood cells throughout an organism’s lifetime. In a screen for epigenetic regulators of hematopoiesis in zebrafish, we identified chromatin remodeling protein and transcription repressor, Ing4, as a requirement for hematopoietic stem and progenitor cell (HSPC) specification. Ing4, a member of the inhibitor of growth (ING) family, is a subunit of the HB01-JADE-hEAF6 histone acetyltransferase complex responsible for most nucleosomal histone H4 acetylation in eukaryotes. Ing4 …

The Deconstruction Of Patriarchal War Narratives In Svetlana Alexievich’S The Unwomanly Face Of War, Liubov Kartashova

Theses and Dissertations

This thesis examines how the Soviet construction of womanhood resulted first in females’ active participation in World War II and then in the silencing of women’s war experiences by fabricating a reality in which women’s trauma did not exist. Such a deprivation of women’s agency led to female soldiers’ confusion of identity, experience of shame and consequential self-censorship. In The Unwomanly Face of War (У войны не женское лицо, 1985), Svetlana Alexievich acknowledges these neglected experiences and traumas, and creates a space in which women’s stories have a right to exist. Applying Jean Elshtain’s theory on the lack of attention …

Enhancing Single And Two-Phase Heat Transfer Of Microgap Heat Sinks Using Nano-Structure Coatings, Yueyang Zhao

Theses and Dissertations

Due to the shrinking size and increasing power of electronics, the heat flux generated by electronic devices has greatly increased and imposed challenges to thermal management technologies. Numerous cooling technologies have been developed to cool high-power electronics. Among them, flow boiling in microchannels or a microgap has been one of the most efficient ways to remove high heat flux, which requires less coolant compared with single-phase cooling techniques. However, the flow boiling instability is the one of the most challenging issues, which lead to the premature critical heat flux (CHF) conditions and deteriorate heat transfer rate. The capillary force generated …

The Association Of Exercise Training Modalities With Circulating Branched Chain Amino Acid And Ketone Body Levels In Patients With Type 2 Diabetes, Ryan Andrew Flynn

Theses and Dissertations

Background: Elevated levels of circulating branched-chain amino acids (BCAA) and ketone bodies are recognized as biomarkers for cardiovascular disease (CVD) and other pathological conditions in type-2 diabetes mellitus (T2DM). Aerobic exercise interventions have been shown to decrease the levels of these markers, suggesting improved metabolic status and reduced risk of CVD. However, the efficacy of resistance training and concurrent programs in reducing BCAA and ketone body levels has not been well researched.

Methods: The current study was performed as a secondary analysis of the HART-D trial, a 9-month randomized, controlled exercise-training trial of 262 participants with T2DM. Participants were randomized …

Expansion Microscopy: A New Approach To Microscopic Evaluation, Ashley Ferri

Theses and Dissertations

Optical microscopy resolution is limited by the wavelengths of light and the series of microscope lenses and other optical components used to create a magnified image of cell structures in a sample. Often the cell structures are smaller or closer together than the resolution limits of a light microscope. In 2015 the Boyden group at the Massachusetts Institute of Technology (MIT) created a sample preparation technique, expansion microscopy which involves embedding biological samples in a crosslinked, swellable, hydrogel polymer that allows for uniform physical separation of cell components so that they can subsequently be resolved by light microscopy. Using the …

The Impact Of Race/Ethnicity On Sentencing: A Matching Approach, Travis Jones

Theses and Dissertations

The purpose of this study is to study the direct impact of race/ethnicity on sentencing of federal drug offenders. In order to accomplish this goal, an exact matching approach is utilized to generate strata containing white, black and Hispanic offenders who are matched based on relevant legal and extra-legal factors derived from focal concerns theory. The total sentences (i.e. fines, probation, incarceration, etc.) of matched offenders are then compared pairwise to determine which offender received the more severe sentence. The findings overall do not suggest that black and Hispanic offenders receive more severe sentences to comparable white offenders; however, drug …

Understanding The Role Of Political Commitment And Coherence Across Sectors And Across National And Sub-National Levels In Enabling Improvements In Nutrition In Rwanda, Elyse Iruhiriye

Theses and Dissertations

Reducing undernutrition is on the political agendas of many low- and middle-income countries. There is general consensus that to improve nutritional outcomes involves actions across the different sectors that influence both the immediate and underlying determinants of optimal nutrition and an enabling environment to support the political and policy processes. Despite renewed attention over the last 15 years, addressing nutrition in a multisectoral way was previously promoted in the 1970’s but failed to have sustained political commitment due to its organizational complexities to plan and implement strategies across sectors.

We conducted an in-depth retrospective case study to understand how Rwanda, …

Antisaccade Performance As A Measure Of Executive Dysfunction In Women With The Fmr1 Premutation, Lyndsay Schmitt

Theses and Dissertations

Women with the FMR1 premutation appear to be at increased risk for executive dysfunction. Findings in this regard have been mixed, leading to controversy surrounding the executive phenotype. Inhibitory deficits have been a more consistently documented component of this cognitive profile (Klusek et al., 2020; Shelton et al., 2014). This study aimed to clarify the executive phenotype through use of the antisaccade task, a well referenced eye-tracking paradigm that targets oculomotor inhibition and motor control and imposes time constraints that may increase sensitivity to executive deficits in women with the FMR1 premutation. The effects of aging were examined in both …

The Struggle Is Real: Exploring Epistemological Change Through The Use Of The Next Generation Science Standards In A High School Chemistry Classroom, Nancy Nasr

Theses and Dissertations

This qualitative action research study examines my resistance to implementing the Next Generation Science Standards (NGSS) in my high school chemistry classroom, which is not uncommon among teachers. This resistance highlighted my conflicting epistemological beliefs, which, as a product of my own experiences as a student, among other factors, are deep-rooted and difficult to change. The dilemma for many science teachers, like me, is that the NGSS approaches the teaching of science in a constructivist manner, which attempts to move science instruction away from traditional pedagogies. Science teachers whose epistemological beliefs are rooted in traditional pedagogies may understand the inherent …

Protein Co-Assembly And Its Application In Enzyme Engineering, Libo Zhang

Theses and Dissertations

Enzymes, as highly efficient biocatalysts, have been researched extensively in both academia and industry because of their distinct advantages including high substrate specificity, high regio- and stereoselectivity, environmentally benign process, etc. Though enzyme catalysis has been scaled up for commercial processes in the pharmaceutical, food and beverage and detergent industries, technical barriers associated with enzyme implementation persist, i.e., low catalytic efficiency at non-natural environment, exhausting product separations, high cost of certain enzymes, etc. Moreover, currently most industrial enzymes are used in single-step reactions; however, multistep and multienzyme catalysis with optimal efficiency could greatly expand its potential for synthetic applications to …

Synthesis And Characterization Of Novel Silicone Hydrogel Biomaterials With Advanced Properties For Ocular Drug Delivery, Liana D. Wuchte

Theses and Dissertations

There is a need for next generation silicone hydrogel biomaterials with greater compositional flexibility and tailorable material properties for advanced manufacturing and new applications for ocular drug delivery. Poly(DMS-R11-co-TRIS-co-DMA-co-PEG200DMA-co-DEAEM-co-DADMAC) silicone hydrogel contact lenses were synthesized with macromolecular memory and showed 7-day in vitro release of latanoprost acid via a microfluidic platform with controlled release and improved drug payload capacity compared to control lenses. Six newly synthesized silicone macromers were then incorporated into contact lens formulations for the first time to produce a novel, improved platform. Various silicone macromer structures showed significantly improved solubility with TRIS and DMA with components ranging …

Applying Psychological Reactance Theory To Intercultural Communication In The Workplace: Dealing With Technological Change And Tolerance For Ambiguity, Oumaima Boulhna

Theses and Dissertations

Psychological reactance theory has yet to be applied to intercultural and cross-cultural communication, at least not to a sufficient extent. This study conducted a cross-cultural examination of psychological reactance in intercultural workplace communication situations. Using the theoretical framework of psychological reactance as well as the constructs of intercultural sensitivity and tolerance for ambiguity, this study expanded applications of PRT for technological change messages in the workplace. The present study extended the previous applications of psychological reactance theory and found a significant cross-cultural variation for trait reactance. The results also revealed that tolerance for ambiguity was negatively related to trait reactance, …

Novel Injectable Plga-Peg-Plga Self-Assembled Hydrogels For The Extended And Controlled Release Of Dna Nanocarriers To Prevent Secondary Cataracts, Laura L. Osorno

Theses and Dissertations

Cataracts are the second leading cause of blindness worldwide. There are over 20 million cataract surgeries each year, and these cases are expected to double within the next ten years. Over 40% of adults and nearly all children develop secondary cataracts, or posterior capsule opacification (PCO), following cataract surgery. Currently, Nd:YAG laser therapy is used to treat PCO; however, laser therapy is not available worldwide and treatment may have adverse effects on surrounding ocular tissues. Thus, there is a considerable unmet need for more efficacious and convenient treatments to prevent PCO.

Injectable, stimuli-responsive gels were designed using poly(lactic-co-glycolic acid)-b-poly(ethylene glycol)) …

Designing Silk Protein-Based Composite Materials With Tunable Heat Transfer And Magnetic Properties, Ye Xue

Theses and Dissertations

Renewable and biocompatible silk protein materials with desired physical properties show promising applications in biomedical field. In this work, a set of protein-based composites with desired thermal and magnetic properties, enhanced by the appropriate distribution of nanofillers in the protein matrix in both 2D-film and 1D-fiber forms was investigated. Results indicate that secondary structures of silk protein materials regenerated by a formic acid-calcium chloride method are different from those of their respective natural silk fibers. Intramolecular beta-sheet structures were found to dominate these silk films, causing these regenerated samples to be water-insoluble but more flexible than traditional silk films with …