Digital Commons Network^™

Gut Microbiome Dynamics In Heart Failure And The Therapeutic Potential Of Nmeg-Cgrp, Kamryn Michael Gleason

Theses and Dissertations

Heart failure (HF) is increasingly recognized as a multisystem disease often linked to gut dysbiosis; however, its specific effects on gut microbial composition remain poorly understood. This study examined long-term changes in the gut microbiome in a murine HF model induced by transverse aortic constriction (TAC) and evaluated the effects of NMEG-CGRP. TAC mimics pressure overload-induced cardiac dysfunction, replicating key features of HF. CGRP, a neuropeptide with vasodilatory and cardioprotective effects, shows potential as a therapy for HF but is limited by rapid degradation. A stabilized analog, NMEG-CGRP, was used to assess its impact on cardiac and gastrointestinal health. Mice …

Performance Limiting Factors For Silicon Anodes In Lithium-Ion Batteries, Najmaddin Bashirzada

Theses and Dissertations

The rising global demand for high-performance and sustainable energy storage solutions has placed lithium-ion batteries (LIBs) at the forefront of technological progress. However, the energy density limits of traditional graphite anodes require the investigation of alternative materials. Silicon (Si), with its high theoretical capacity, is a promising anode material. Still, its practical use faces major challenges such as volumetric expansion, unstable solid electrolyte interphase (SEI) formation, and low conductivity. This thesis examines the electrochemical behavior and limitations of Si-based electrodes using coin cells and ex-situ three-electrode cells. A comparison of electrochemical testing methods – i.e., Galvanostatic Intermittent Titration Technique (GITT) …

Identifying And Understanding The Barriers To Sexual Health Information Seeking Experienced By Young Adults (Ages 18-26) Using Social Media, Alexander Nicolas Vera

Theses and Dissertations

This dissertation uses a qualitative, constructionist approach to examine the sociotechnical and cultural dynamics that shape digital sexual health-seeking practices of young adults. Guided by information practices and affordance theory, the research draws on semi-structured interviews and digital walkthroughs with 20 young adults (ages 18–26) from a convenience sample of young adults residing in South Carolina. Participants described how they use platforms like TikTok, Instagram, and Reddit not only to locate information but to assess its trustworthiness, inclusivity, and personal relevance in real time. Through in-depth accounts and screen-based demonstrations via digital walkthroughs, the study examines how participants engage in …

Development Of A Strategy To Neutralize Ccl8 Activity And Ablate Ccl8 Cellular Targets, Bernardo Chavez

Theses and Dissertations

The chemokine CCL8 (monocyte chemoattractant protein-2) is a versatile signaling molecule involved in tumor progression, immune cell recruitment, and therapeutic resistance. Through activation of receptors including CCR1, CCR2B, and CCR5, CCL8 promotes leukocyte trafficking, epithelial–mesenchymal transition, and the establishment of immunosuppressive tumor microenvironments. In breast cancer and inflammatory diseases, elevated CCL8 levels correlate with poor clinical outcomes, making it a promising therapeutic target.

A strategy was developed to block CCL8 activity and selectively ablate CCL8 receptor expressing cells. A neutralizing monoclonal antibody, 1G3E5, was identified from a panel of murine candidates based on high affinity CCL8 binding. Sequencing revealed two …

The Role Of Fibroblast Activation Protein On Left Ventricular Growth And Extracellular Matrix Remodeling As A Function Of Age And Pressure Overload, Amelia Marie Churillo

Theses and Dissertations

Fibroblast activation protein (FAP) is abundantly expressed in cancer associated fibroblasts and contributes to extracellular matrix (ECM) remodeling. Recent studies using imaging techniques have identified increased levels of FAP in the left ventricle (LV) of patients identified as at risk for developing heart failure. However, the role of FAP in regulated LV myocardial structure and function remains unclear. This project tested two hypotheses: First, genetic ablation of FAP would modify LV structure and function and be more pronounced with aging. Second, genetic ablation of FAP would modify LV structure and function with LV pressure overload (LVPO) accompanied by ECM remodeling.

Multi-Layer Decision Making For Long-Term Autonomous Mission Based On Dual Process Theory, Shruti Jadhav

Theses and Dissertations

Unmanned aerial vehicles (UAVs) are increasingly used in precision agriculture, where extended autonomous operation is required for monitoring, intervention, and field management. However, achieving long-term autonomy remains challenging due to battery constraints, environmental uncertainty, and the need to balance exploration with event-driven tasks. To address these challenges, a multi-layer decision-making framework inspired by Dual Process Theory (DPT) is developed. The framework combines reactive return-tobase strategies, exploratory navigation, and directional bias from prior missions, with a conflict-monitoring mechanism that adapts system behavior based on real-time conditions. The approach is implemented in a simulated agricultural grid environment, demonstrating improved adaptability and coverage …

Multiscale Modelling Of Eg5: Structural Insights Into Microtubule Interactions, Atp Hydrolysis, And Allosteric Inhibition Mechanisms, Soundarya Priya Alexandar Ms

Theses and Dissertations

The mitotic kinesin EG5 is essential for spindle bipolarity and accurate chromosome segregation during cell division, making it a validated anti-cancer target. Unlike conventional microtubule-targeting agents, EG5 inhibitors offer mitosis-specific action, reducing neurotoxicity. Although inhibitors targeting EG5’s allosteric site-I have been extensively studied, mechanistic insights into inhibition at site-II, and their combined effects, remain underexplored. This thesis systematically investigates the structural and dynamic mechanisms underlying EG5 function and its inhibition using an integrative computational approach.

Conformational analysis, coarse-grained dynamics, all-atom molecular dynamics simulations, and protein network analysis were performed on EG5 motor domain structures and EG5-microtubule complexes. Results show that …

Functional Role Of Cysteine Residues In Vtlr Of Agrobacterium Tumefaciens, Suravi Islam

Theses and Dissertations

Agrobacterium tumefaciens is a soil-dwelling plant pathogen known for transferring DNA to host plants through a type IV secretion system. Central to its virulence and cellular regulation is the LysR-type transcriptional regulator, VtlR, which controls processes including biofilm formation, motility, tumorigensis, and small RNA expression. Although VtlR’s regulatory role is established, the specific functions of its conserved cysteine residues have only recently begun to be explored. This study investigates VtlR’s four cysteines (C144, C236, C244, and C273) through site-directed mutagenesis and a combination of phenotypic assays and transcriptional profiling. Phenotypic assays confirmed that C144 and C273 are important for proper …

Assessing Inclusion Within Naaee Affiliate Ee Associations And Where To Go From Here, Connor Chilton

Theses and Dissertations

Environmental Education (EE) has a history that has been typically defined by traditionally dominant identities (White, male, able-bodied, etc.). The North American Association for Environmental Education (NAAEE) offers a unique position as frontrunners for the field of EE and have the duty to provide an inclusive-minded education for and about the environments throughout the world. Through NAAEE’s affiliate network within the United States, this paper details a glimpse into the work towards inclusion by surveying its leadership position holders about the broad subject and several interconnections of inclusion. Upon completion of the survey, participants who opted to participate in a …

Ground-Based Modeling Of Deep-Space Risks To The Spleen And Intestine In Mice, Edith Nathalie Pineda Sotomayor

Theses and Dissertations

Deep-space missions, such as those planned for the cis-lunar space and Mars, pose unique challenges to astronaut health due to prolonged microgravity (MG) exposure and low-dose chronic irradiation (CIR). Understanding the impact of these stressors on secondary lymphoid tissues, such as the spleen and intestine, is crucial for ensuring crewmembers' well-being, given the impracticality of conducting this research with human samples. Using a ground-based facility, we exposed C57BL/6 female and male mice to CIR (1.74 Gy at a dose rate of 0.0029 Gy/h) and/or simulated MG (SMG) via hind limb unloading (HLU) for one month. Spleen and intestinal tissues were …

Punitiveness In The Modern Era: Gender Roles & Anti-Transgender Attitudes, Haley Brooke Trantham

Theses and Dissertations

Public opinion on punitiveness is an important topic of study because it can influence political elites’ decision making when developing legislation. This study focused on the role gender ideology and anti-transgender attitudes on punitiveness while controlling for religious and political conservatism. The 2020 American National Election Study (ANES) was used to examine the association between conservatism, traditional gender roles, and anti-transgender attitudes on their influence of punitiveness. Three ordinal logistic regressions were run for each type of punitiveness (i.e., death penalty support, crime spending, and felon disenfranchisement). Results indicated that religious conservatives had lesser odds for supporting the death penalty …

Analyzing The Role Of A Dual-Function Diguanylate Cyclase / Phosphodiesterase For Attachment And Motility In Agrobacterium Tumefaciens, Priya Aryal

Theses and Dissertations

Cyclic diguanylate monophosphate (c-di-GMP) is a second messenger that is responsible for attachment and motility in many prokaryotic systems and controls the signaling pathway for the motile to sessile transition in many bacteria. Many c-di-GMP-metabolizing proteins are diguanylate cyclases (DGCs), responsible for the synthesis of c-di-GMP and characterized by a c-di-GMP synthesizing domain, e.g., GGDEF domain. Other c-di-GMP-metabolizing proteins contain c-di-GMP degradative domains, e.g., EAL, characterized by phosphodiesterase activity (PDE). In Agrobacterium tumefaciens, out of 31 predicted proteins regulating the c-di-GMP level, this project focuses on the predicted dual-function DGC/PDE, DcpB. We hypothesized that DcpB plays a role in the …

Examining The Role Of The M1 Motif Of Abcr1 Srna In Agrobacterium Tumefaciens, Daniel Arango-Cardona

Theses and Dissertations

Agrobacterium tumefaciens is a gram-negative alphaproteobacterium that is one of the causative agents of crown gall tumors on plants. Its ability to transform plants using its T-DNA has been studied to improve plant-mediated transformation. sRNAs are small regulatory RNAs that play a role in gene expression in a variety of organisms and bind to target proteins or mRNAs. A. tumefaciens contains two sibling sRNAs, called AbcR1 and AbcR2, which are conserved among alphaproteobacteria. A. tumefaciens AbcR1 and AbcR2 are closely related, exhibiting 69% sequence identity, similar secondary and tertiary structure, and a conserved motif, M2, believed to direct target interactions. …

Multi-Fidelity Machine Learning Modeling For Aerodynamic Response Prediction Of Aerospace Vehicles, Ethan S. Jackman

Theses and Dissertations

Hypersonic vehicle design requires understanding complex aerodynamic phenomena across the full flight regime. This study presents a novel MF surrogate modeling methodology that enables the prediction the full field response across a vehicle’s surface. A Space-Filling Curve (SFC) is used to convert unstructured data into 1D vectors. The a Convolutional Autoencoder is used with transfer learning to reduce the dimensionality of the data. An Emulator-Embedded Neural Network (E2NN) combines multi-fidelity data for fast, accurate predictions. A benchmark analytical example and hypersonic application are used to evaluate the methodology. Using various numbers of samples and sampling strategies it is found that …

An Antioxidant Approach To Ameliorate The Effects Of Radiation And Chemotherapy On Cognition, Raymond Anan Otoo

Theses and Dissertations

Acute Lymphoblastic Leukemia (ALL) is a hematologic malignancy that primarily affects children and young adults. While advancements in treatment—including chemotherapy and cranial irradiation—have significantly improved 5-year survival rates in children to nearly 90%, radiation-induced cognitive impairment (RICI) remains a persistent and debilitating side effect. The brain, particularly the hippocampus, is highly susceptible to radiation-induced oxidative stress and inflammation. In the treatment of ALL, the combined use of chemotherapy and radiotherapy contributes to a heightened oxidative burden, exacerbating the risk of cognitive dysfunction. Although previous studies have linked radiation exposure to neurocognitive decline, the molecular mechanisms underlying RICI and chemotherapy-induced cognitive …

Rethinking The Red Zone: Risk Factors Of College Student Dating Violence Victimization During The Fall Semester, Alexandra Nicoletti

Theses and Dissertations

College students experience dating violence (DV) victimization at alarmingly high rates, which is associated with a variety of negative outcomes (Brewer & Thomas, 2019; Choi et al., 2017; Leemis et al., 2022). To implement effective prevention programming, it is important to understand the risk factors associated with DV victimization on college campuses. Further, college students report the highest rates of one type of DV victimization—sexual victimization—during the fall semester, also known as the “red zone” (Carey et al., 2018, Cranney, 2015). This study utilized the socioecological model to understand which individual-, relationship-, and community-level risk factors were associated with DV …

Development Of Novel Small Molecules As Potential Anti Cancer Agents, Robert John Chitren

Theses and Dissertations

This doctoral thesis explores the discovery and characterization of two novel small molecules, designated as KS100 and (R,S’)-MNF, with potential anti-cancer properties. The study encompasses a comprehensive investigation into the molecular mechanisms underlying the anti-cancer effects of these compounds, as well as their therapeutic potential in preclinical models. These findings highlight the potential of KS100 and (R,S’)-MNF as lead candidates for cancer therapy, paving the way for further preclinical development and clinical translation.

Engineering Protein Linkers For Optimized Telsam-Darpin Fusion And Crystallization With Electric Field-Based Polymer Orientation, Maria Jose Pedroza Romo

Theses and Dissertations

Fusing a variant of the sterile alpha motif domain of the human translocation ETS leukemia protein (TELSAM) to a protein of interest significantly enhances crystallization and diffraction quality. TELSAM is a pH-dependent, polymer-forming crystallization chaperone that, when covalently fused to a protein, forms a well-ordered crystal lattice. However, the success of this method depends on two factors: 1) the design of the linker connecting TELSAM to the target protein, which influences both crystal packing and target orientation, and 2) the prevention of TELSAM polymer flipping, a phenomenon that disrupts lattice periodicity and impairs structure determination. To address the first challenge, …

Cost-Effectiveness Model For Reservoir Sediment Management Economics, Connor Ronald Hale

Theses and Dissertations

Reservoir sediment deposition poses a challenge to sustainable water supplies around the world. Sediment management has proven to be an expensive endeavor. Two models have been developed to guide economic analysis for reservoir sediment management, namely the Reservoir Sediment Economics Model (RSEM) and the Reservoir Conservation Model 2 (RESCON 2). While these are helpful tools for benefit-cost prefeasibility studies, they rely heavily on sometimes elusive estimations of the benefits of water supply. In this study, a new method for economic analysis is proposed that foregoes benefit-cost analysis in favor of a cost-effectiveness approach. The Sediment Management Cost-Effectiveness Model ranks sediment …

Satellite-Based Identification Of Mesoscale And Submesoscale Eddies And Fronts In The Gulf Of Mexico, Ethan Cruz

Theses and Dissertations

Mesoscale and submesoscale processes in the Gulf of Mexico (GoM) are fundamental in shaping the region’s physical and biogeochemical ocean dynamics. Advances in high-resolution satellite observations now allow detailed detection of submesoscale (< 25 km radius) and mesoscale eddies and ocean fronts, which drive heat, nutrient, and carbon fluxes. This dissertation integrates two complementary investigations: (1) a comparison of eddy-tracking algorithms: Contour Tracing (CT) and Temperature Thresholding (TT), applied to Optimum Interpolation Sea Surface Temperature (OISST, 1/4°) and Operational Sea Surface Temperature and Ice Analysis (OSTIA, 1/20°) products; and (2) a comparative evaluation of three ocean front detection algorithms: Canny Edge Detection (Canny), the Cayula–Cornillon Algorithm (CCA), and the Belkin–O’Reilly Algorithm (BOA), across multiple satellite-derived parameters, including sea surface temperature (SST), sea surface salinity (SSS), chlorophyll-a (Chl-a), and altimetry-derived absolute dynamic topography (ADT). The eddy-tracking analysis shows that the higher-resolution OSTIA consistently detected more eddies than OISST, with TT performing best during winter when SST gradients were strongest. The front detection analysis, incorporating datasets from the Surface Water and Ocean Topography (SWOT) and Plankton, Aerosol, Cloud, Ocean Ecosystem (PACE) missions, was evaluated under diverse conditions, including flood and drought periods, Loop Current phase changes, and extreme weather events. A machine learning–based Gaussian Mixture Modeling (GMM) approach was also developed, providing a novel framework for dynamic front detection. By benchmarking traditional and machine learning methods, this integrated study identifies optimal algorithm–dataset combinations for resolving submesoscale and mesoscale features in the GoM, with applicability to other regions.

Köppen-Geiger Climate Effects On F-15 Readiness Spares Package Parts, Maximus A. Fan

Theses and Dissertations

Readiness Spares Packages (RSP) are critical to deployed operations. Future demands of the Air Force require squadrons to operate in different climate environments from home stations. RSPs can sustain aircraft maintenance operations for up to 30 days. Currently, failure rates of parts within the RSP are assumed to be constant. This research aims to explore whether there is a difference in F-15 RSP failure rates when Koeppen climate classifications are taken into effect. The Koeppen-Geiger system classifies area climates based on the geography, elevation, and location. The history of operations and diversity of F-15 locations make the aircraft an ideal …

Magnetic Ordering And Dynamics In Assemblies Of Magnetite Nanoparticles Throughout The Superparamagnetic Transition, Johnathon Michael Rackham

Theses and Dissertations

Magnetic nanoparticles are used in nanotechnologies and biomedical applications, such as drug targeting, hyperthermia treatment, MRI contrasting agents, and bio-separation of compound solutions. Magnetite (Fe3O4) nanoparticles stand to be effective in these roles due to the non-toxic nature of magnetite and its strong magnetization. To this end, a greater understanding of the magnetic behavior of the individual magnetite nanoparticles is needed when a collection of them is used. This research seeks to discover the local magnetic ordering and dynamics of fluctuations of ensembles of magnetite nanoparticles at various stages of the magnetization process, throughout the superparamagnetic blocking transitions, and for …

Re-Engaging Driver Attention Using Chatgpt: A Multimodal Study On Stress Impact And Driving Performance, Alaa Zaki Elfiqi

Theses and Dissertations

Driving is considered a complex task that requires continuous focus and attention from the driver. Driving performance can also be impacted by changes in the driver’s stress level. However, limited research explores methods to address this challenge. With the current advances in Large Language Models (LLMs) and their ability to engage in human-like interaction, this study investigates the potential of using ChatGPT, designed to speak Egyptian Arabic, in re-engaging driver attention under different driving scenarios for low-stress and high-stress conditions within a virtual reality (VR) environment driving simulator. Drivers were asked to follow a leading car into two scenarios with …

Exposure To Zinc Oxide Nanoparticles Reveals A Death Resistance Mechanism In Dictyostelium Discoideum, Zoya Nadeem Faruqui

Theses and Dissertations

Zinc oxide nanoparticles (ZnO NPs) are very versatile due to their multifunctional properties including unique physicochemical and antimicrobial characteristics. They are widely utilized across various fields such as biomedical, industrial fields, environmental remediation, and agriculture. Therefore, their cytotoxic effects on biological systems necessitate a deeper understanding of the cellular responses to nanoparticle exposure. This project primarily investigates the impact of ZnO NPs on the model organism Dictyostelium discoideum AX4 (social amoeba) which has not been examined before unlike a vast variety of organisms in the literature. The cytotoxic effects of ZnO NPs on Dictyostelium discoideum AX4 cells are compared with …

The Impact Of Interactive Read-Alouds On Students’ Literacy Development, Kaitlyn Hill

Theses and Dissertations

This thesis examined how multicultural interactive read-alouds support first-graders’ literacy skills and cultural awareness. Multicultural literature was used to conduct interactive read-alouds to first-grade students. A look across all data sources suggested three main themes that occur throughout the research study. These themes included student engagement and questioning, making connections by experiencing culture through an authentic lens, and developing cultural awareness and empathy for those who are different. It was evident that incorporating multicultural literature into classroom instruction had a positive impact on students’ literacy development and cultural awareness. The data suggested that these multicultural texts supported the students’ development …

A Contemporary Approach For Exploring The Influence Of Detoxification And Standardization Of Traditional Metallopharmaceutical Product Pharmacological And Toxicological Aspects, Malarvizhi K

Theses and Dissertations

Polyherbomineral formulation possess unique medicinal properties due to the presence of metals and minerals as integral part, as processed in addition to specific herbals. There is an increased interest in metallopharmaceuticals in clinical and research areas, because of their immense therapeutic efficiency towards multiple diseases and evidence for non-toxic claim. Sivanar Amirtham is one of the polyherbomineral Siddha medicine recommended for the treatment of various respiratory diseases and other ailments including antidote therapy for poisonous bites. The present research work attempted for standardization of Sivanar Amirtham preparation as per the traditional standard protocols (including the detoxification process of raw materials) …

Magnetic Molecularly Imprinted Polymers For Quercetin Adsorption: Fabrication, Characterization, And Application, Vinitha Ug

Theses and Dissertations

A highly selective Magnetic Molecularly Imprinted Polymer (MMIP) was developed for the detection and purification of Quercetin. The Density Functional Theory was utilized for the selection of suitable monomers based on binding energy interaction between quercetin and monomers (Acrylamide, Acrylonitrile, Methacrylic Acid). From computational studies, Acrylamide was selected as a suitable monomer for the synthesis of MMIP. Under ultrasound irradiation both quercetin and naringin imprinted materials MMIP-Q and MMIP-N were fabricated.

Compared to ultrasound-assisted synthesis, Microwave Synthesis provides uniform heat transfer throughout the reaction solution, which enables uniformity in the size of synthesised material, increases the yield of material and …

Facile Fabrication Of Protein-Polysaccharide Conjugate Multifilament Nerve Guidance Conduit For Peripheral Nerve Regeneration, Preethy Amruthavarshini R

Theses and Dissertations

Long segmental defects in peripheral nerves impair sensory and motor function by disrupting neural impulse transmission. End-to-end autologous grafting with proper fascicular complementation has demonstrated functional recovery per the Medical Research Council Classification (MRCC). However, obtaining donor nerve tissue exceeding 30 mm remains a significant challenge. Artificial hollow conduits serve as an alternative but are limited to defects smaller than 30 mm due to inadequate innervation across the lumen.

Multichannel nerve guidance conduits (mNGCs) have emerged as a promising solution by enhancing fascicular complementation similar to autografts. This study presents a two-step approach for fabricating nerve conduits with precise fascicular …

In Silico Investigations Of Class Ii Trans Activator (Ciita): Its Plausible Implications In Neuroinflammation And Neurological Disorders, Kishore N

Theses and Dissertations

Inflammation plays a significant role in maintaining homeostasis. Neuroinflammation is a chronic inflammatory response in the central nervous system (CNS), leading to brain or spinal cord dysfunction. To promote mental health, it is crucial to understand the regulation of neuroinflammation. Class II transactivator (CIITA) is a master-regulator of the major histocompatibility complex II (MHCII).

Modulation of CIITA has been suggested as a promising intervention for inflammatory conditions, including neurological disorders. However, there is limited knowledge about the role of CIITA and its interactors in inflammation. This study aimed to understand the role of CIITA in neuroinflammation and neurological disorders using …

3d Bioprinting On Electrospun Nanofibrous Scaffolds To Fabricate Myocardial Patches, Muthu Parkkavi S

Theses and Dissertations

Tissue-engineered three-dimensional (3D) myocardial patches offer superior and innovative treatment strategy for myocardial infarction (MI). Among the various scaffold fabrication techniques, electrospinning and 3D bioprinting have received great attention due to their ability to recreate native cardiac tissue mimetic characteristics. Despite several advantages, electrospun nanofibers have limitations in cell delivery due to random cell seeding, while bioprinting faces challenges in creating hydrogel constructs with ideal mechanical properties capable of supporting cardiac contraction/relaxation cycle. Hence in this work, hybrid myocardial patches were developed by converging electrospinning and bioprinting techniques.

Electrospinning of PLCL:PEOz (B73, 7:3 blend of poly-L-lactide-co-ε-caprolactone (PLCL) and polyethyl oxazoline …