Digital Commons Network^™

Applicability Of Cementochronology To Estimate Season-Of-Death In Humans: Insights From Modern Documented Collections, Olga Ibarra-Sanchez

Theses and Dissertations

Dental cementum is characterized by lifelong deposition of alternating opaque annuli in fall/winter and translucent bands in spring/summer. This study aims to test the applicability of cementochronology to estimate season-of-death, by observing the properties of the last layer, with direct implications for forensic anthropology and bioarchaeology. The sample includes canine teeth from 146 adults (84 males, 62 females), age-at-death ranging from 20-97 years (mean=61.7 years, SD=18.6 years), from two contemporary skeletal collections. Of these individuals, 78 (53.4%) died in spring/summer and 68 (46.6%) died in fall/winter. Overall accuracy was low at 64.4% (94/146). Accuracy was higher for spring/summer deaths 100.0% …

The Role Of C-X-C Chemokine Receptor Type 5 (Cxcr5) On Sex-Dependent Neurobehavioral Changes In The Sub Clinically Sensitized Individuals With Repeated Allergen Exposure, Dilini Vimarshani Ekanayake Ekanayake Mudiyanselage

Theses and Dissertations

Food allergen consumption has been suspected to influence mood and behavior in individuals who are sensitized but tolerant to the food. Using a mouse model of subclinical cow’s milk allergy (CMA), we previously showed that C57BL/6J mice sensitized to β-lactoglobulin (BLG, Bos d 5) exhibited elevated BLG-specific IgE levels even without observable allergic reactions, suggest developing immune responses towards the allergen. We also observed elevated cytokine and chemokine levels, astrogliosis and cortical demyelination along with behavioral changes such as anxiety-like and depression-like behavior after consuming a whey-containing diet for two weeks. Furthermore, we demonstrated the possible immune cell infiltration and …

Microbial Communities And Host Plants Of Megachilidae In The Greater Grand Forks Area (Ggfa), Sara Margit Wallace

Theses and Dissertations

Although Megachilidae are important pollinators in the United States, there is still much to learn about the health and functionality of these bees. Megachilids are threatened by competition from non-native pollinators and other environmental factors. The species distribution and composition of the gut microbiome in megachilid bees are not well known for the Greater Grand Forks Area (GGFA). My research seeks to answer the questions: what megachilid species are in the GGFA, where are they, and what symbionts are in their gut microbiomes? I collected and identified solitary bee specimens from North Dakota and Minnesota, dissected their gut contents, and …

Testing Microscopic Approaches For Cementochronology In The 21st Century Identified Skeletal Collection, Portugal, Amando Luis Montalvo Jr.

Theses and Dissertations

Age-at-death estimation is a key component to the creation of a biological profile in forensic anthropology. Acellular extrinsic fiber cementum annulations have been shown to correlate with chronological age. Cementochronology examines this correlation through the sum of the alternating translucent bands and opaque annuli, and the age of eruption of a tooth. While several microscopy techniques have been used, there is no consensus on the most optimal. This study will assess the reliability, precision and accuracy of three microscopy techniques: Brightfield (BF), Phase Contrast (PC) and Differential Interference Contrast (DIC). PC was the most reliable (rTEM= 10.4%), the most precise …

Benthic Infaunal Responses To Shoreline Restoration, Jessica Lauren Cline

Theses and Dissertations

In the age of increasing urban coastal sprawl, natural shorelines are being replaced by hard armoring structures, which result in the loss of valuable ecosystem services. Hybrid living shorelines, areas that include limited and less intensive armoring strategies paired with the integration of plants and other natural materials, present an opportunity to marry the need for more aggressive stabilization with the continued preservation of ecosystem functions and biological communities. This project uses benthic infauna to evaluate the success of a living shoreline installation in Palm Bay, Florida. The deployment features experimental treatments of breakwaters and red mangroves to explore how …

Navigating Structure, Function, And Stability: Bridging Immunotherapy And Protein Engineering In The Era Of Machine Learning, Christopher J. Haynie

Theses and Dissertations

The immune system is a remarkably adaptive network, yet it often fails to function optimally due to evasion strategies of pathogens or mutated cells. Many of these evasion strategies involve alterations in protein expression and function. Protein design is an area of computational biology with potential applications in improving protein function and therapeutic protein engineering. This thesis explores methods to enhance immune function and protein design across multiple scales, from molecular engineering of antibody fragments and chimeric antigen receptors (CARs) to cellular immune responses, and computational approaches for protein structure optimization. We characterize Thymidine Kinase 1-specific single-chain antibodies, highlighting both …

Copper Oxide Based P-Type Chemiresistive Sensing Element And Electrochemical Electrode For Ethanol Detection Application, Reyoun Frances B

Theses and Dissertations

This study centers on the development of nanostructured sensing elements and electrochemical electrodes based on p-type CuO for ethanol detection, with a specific focus on application related onto targeting Breath and Blood Alcohol Content (BAC) monitoring. CuO nanostructures were deposited onto ceramic tubes via spray pyrolysis under optimized conditions to ensure uniform film formation. The CuO surfaces were subsequently functionalized with silver (Ag) nanoparticles via thermal evaporation and reduced graphene oxide (rGO) using the doctor blade method. Structural, morphological, and compositional analyses were conducted using X-ray Diffraction (XRD), Field Emission Scanning Electron Microscopy (FESEM), and X-ray Photoelectron Spectroscopy (XPS), respectively. …

Life After Prison: Exploring Gendered Differences In The Impact Of Social Support On The Ease Of Reintegration For Previously Incarcerated Men And Women, Joy Ferdinand

Theses and Dissertations

Successful reintegration into society remains a significant challenge for formerly incarcerated individuals, particularly in securing stable housing and employment. This study examines the gendered impact of social support on reintegration outcomes, specifically assessing how peer instrumental support, familial instrumental support, and familial emotional support influence housing stability and employment within three months of release. Using data from the Serious and Violent Offender Reentry Initiative (SVORI), this study employs logistic regression models to explore whether gender moderates the relationship between social support and reentry success. Findings indicate that peer instrumental support significantly affects housing stability, but its impact varies by gender. …

Design And Implementation Of Home Energy Management System (Hems) In Smart Grid Through Demand Side Management (Dsm), Nirmala Jegadeesan Ms

Theses and Dissertations

The rising global energy demand, combined with environmental concerns and the depletion of conventional energy sources, has driven the need for the development of smarter and more sustainable energy systems. This research focuses on Home Energy Management System (HEMS) using soft computing techniques to optimize load scheduling, enhance energy efficiency, and integrate Renewable Energy Sources (RES) such as solar and battery storage. The primary goal is to balance supply and demand, reduce peak loads, and minimize energy costs while ensuring consumer comfort and system reliability.

Smart grids provide enhanced capabilities compared to conventional grids by enabling bidirectional flows of energy …

Using An Old Assay In New Ways: Differential Scanning Calorimetry Characterization, Quantification, And Interaction Analysis Of Whey Proteins, Charity Conlin Jennings

Theses and Dissertations

Whey proteins including β-Lactoglobulin, α-Lactalbumin, Immunoglobulin-G, Serum Albumin, Lactoferrin, and Lactoperoxidase each have unique biological properties spanning anti-bacterial, anti-fungal, and anti-viral activities as well as deliver essential nutrients to the human body. In the past decade, there has been a large focus on extracting these individual proteins for their use in foods and supplements. In order to best extract these proteins and prepare them properly in foods and supplements, an understanding of how they behave and denature in different environments is necessary. However, there is insufficient knowledge of their thermodynamic properties and potential interactions in varying conditions. Additionally, there are …

Development Of Natural Product Analogs As Therapeutic Agents, Jacob Mara

Theses and Dissertations

Cancer stem cells (CSC) are cancer cells in a tumor mass that may self-renew and develop into various cell types, resulting in a heterogeneous tumor. CSCs have been shown to have a role in many facets of cancer formation, including tumor genesis, proliferation, and metastatic activity. They are also implicated in chemotherapeutic treatment resistance and the recurrence of some malignancies. Based on these capacities, CSCs have been identified as the next target for cancer therapy and control. Salinomycin's potential for therapeutic use based on ongoing research, as well as the synthesis of its derivatives and their biological activity, will also …

Examining Choices Of Sarcastic Responses In Dialogue With Virtual Interlocutors, Samantha Langley

Theses and Dissertations

We report the results from three experiments examining people’s preferences for using sarcasm in conversation as a function of: 1. whether their interlocutor uses sarcasm during the conversation; 2. Their knowledge about whether the interlocutor has used sarcasm in a previous conversation; 3. The presence of prosodical and visual cues for interlocutors’ sarcasm. In each experiment, participants took part in two conversations, and we manipulated whether the interlocutors were sincere or sarcastic and whether they remained the same or changed from one conversation to the next. Experiment one used written conversations and experiment two and three used pre-recorded video clips …

Leveraging After School Programs As Resources To Support Adolescent Health And Wellbeing Within Underserved Communities: A Qualitative Study, Olivia Staples

Theses and Dissertations

Over the past decade, youth have shown a consistent rise in stress, major depression, anxiety, and suicide, with underserved youth (low-income, minoritized communities) at greatest risk of exposure to chronic stress, related outcomes, and less access to health services (APA, 2017). Afterschool programs (ASPs), which serve as important resources in underserved communities, can be a critical support for youth health and well-being (Afterschool Alliance, 2019). However, few studies have examined ways in which ASPs address the challenges underserved youth face across their ecosystem to promote youth resilience (Jones & Deutsch, 2011). Using qualitative interview data from staff and youth across …

Stem Integration In The Classroom: An Exploratory Case-Study On Teachers Defining Their Stem Identity And Role As A Stem Educator, Tawana Guillaume

Theses and Dissertations

The push for inclusion of science, technology, engineering, and mathematics (STEM) as a meta-discipline in K-12 education is recognition that the challenges faced by society require technology skills, interdisciplinary problem-solving, and an increased number of people entering STEM professions (Dare et al., 2014; English, 2016; Fan et al., 2021; Holstein & Keene, 2013; Roehrig et al., 2012, 2021; Sanders, 2008). The dissertation in practice explored STEM teacher identity and conceptions of STEM as a function of STEM professional experience outside of the field of education. The exploratory casestudy sought to identify the relationship between STEM career-based experiences – specifically the …

Improved Extraction And Detection Of Disinfection Byproducts, Impacts Of Wildland-Urban Interface Wildfires On Drinking Water, And Change In Disinfection Byproducts Over Time In Chlorinated Vs. Chloraminated Distribution Systems, Patrick Thomas Justen

Theses and Dissertations

Drinking water disinfection is critical to prevent the occurrence of waterborne pathogens in drinking water. However, disinfection causes the unintended formation of disinfection byproducts (DBPs) through the reaction of disinfectants (e.g., chlorine, chloramine) with natural organic matter (NOM), algal matter, or anthropogenic pollutants present in source waters. Exposure to DBPs is associated with increased bladder cancer, colorectal cancer, miscarriage, and birth defects. The U.S. EPA currently regulates eleven DBPs in drinking water—four trihalomethanes, five haloacetic acids, bromate, and chlorite. However, research shows that DBP related toxicity in drinking water is driven mainly by other, non-regulated DBPs, particularly iodinated DBPs and …

Inspiring Girls To Fill The Blank Space In Stem: The Impact Of An After-School Coding And Robotics Club On Elementary Female Students' Engagement And Self-Efficacy In Computer Science, Lisa Guenther Wilbanks

Theses and Dissertations

The purpose of this action research was to describe how participation in an after-school coding and robotics program influenced elementary female students’ engagement and self-efficacy in computer science. According to a 2023 report from the National Science Foundation, in 2020 women earned only 21% of computer science degrees and 24% of engineering undergraduate degrees in the United States. As two of the fastest growing fields, the lack of female perspectives and experiences will continue to limit innovation in these areas. Studies show that gender gaps in engagement and self-efficacy in STEM begin long before students enter college and the workforce, …

Adipokines Mediate Fat-To-Ovary Communication To Regulate Oogenesis, Adipocyte Size, And Lipid Storage In Drosophila Melanogaster, Chad Herbert Simmons

Theses and Dissertations

Humans have complex signaling pathways that allow them to store and expend energy based on nutritional quality and availability. Inter-organ signaling is used to communicate nutrient availability between organs based on nutritional needs and the rate of cellular division. Our main overarching question is to investigate how fat-derived signals affect adipocyte morphology and the maintenance of stem cells in the ovary. While signaling pathways, like insulin and mTOR, and some adipokines that are involved in inter-organ nutrient sensing have been identified, the exact nutrient reliant mechanisms of stem cell maintenance are not completely understood. Using Drosophila melanogaster as a model, …

Ai-Driven Autonomous Manufacturing: A Novel Taxonomy, Vision-Guided Planning, And Diversity-Aware Active Learning Frameworks, Ibrahim Yousif

Theses and Dissertations

Manufacturers face two opposing challenges: the escalating demand for customized products and the pressure to reduce lead times. Current manufacturing equipment, although reliable, operates at the limits of its technology and lacks adaptability to dynamic environments. This trade-off has been described as the optimal degree of automation, a threshold beyond which further automation incurs more cost than benefit. Smart manufacturing has introduced adaptive, data-driven systems, but many deployments still lack the contextual adaptability and autonomous decision-making required to handle unplanned disruptions. This underscores the need for intelligent, autonomous systems capable of real-time adaptation without costly reconfiguration, minimizing human intervention during …

Multiscale Geometric Analysis In Endovascular Therapies: Ex Vivo, In Silico Approaches, Dima Hussein Ali Bani Hani

Theses and Dissertations

In this work, we address a persistent global challenge of vascular disease, specifically peripheral artery disease (PAD), and arteriovenous fistula (AVF) complications in patients with end-stage kidney disease (ESKD). The important role of vascular geometry in disease intervention plans and the development of endovascular therapeutic strategies (e.g., Drug-coated balloon (DCBs)) is critical in addressing these challenges.

This study evaluates the feasibility of co-delivery of paclitaxel (PTX) and valsartan (VAL) using urea-based coatings in DCB, analyzes the coating morphology and microstructural changes to improve therapeutic results. A computational finite element model was developed to complement experimental work, simulate tissue-coating interactions, compute …

Investigation Of Metals And Incidental Nanomaterials In Wildland-Urban Interface Fire Ashes And Their Impacts On Marine Phytoplankton, Talal Abdullah Alshehri

Theses and Dissertations

Wildfires are natural processes that have played a vital role in ecosystems for millions of years. However, with increasing anthropogenic activities and climate change, wildfires' frequency and intensity have increased. A wildfire is an uncontrolled fire that burns forests, grasses, and savannas. In addition to vegetation combustible materials, wildfire burn structure items such as homes, stores, and storage sheds in areas where the transition zone between urban and wildland is defined as the wildland-urban interface (WUI). Moreover, wildfires in WUI can burn vehicles, including automobiles, tractors, and boats on trails. Recently, there have been concerns regarding the metals, metalloids, and …

Predictors Of Outpatient Follow-Up Attendance And Subsequent Psychiatric Readmission, Sandra J. Beltran

Theses and Dissertations

Psychiatric readmissions remain a persistent challenge in mental health care, often reflecting gaps in continuity of treatment following discharge. The Agency for Healthcare Research and Quality (2019) indicated that a key component in preventing inpatient readmission is adequate outpatient care following discharge. Moreover, early attendance in outpatient follow-up visits is associated with improved patient outcomes (Boston Medical Center, 2022). Various predictors are associated with engagement in outpatient services, such as age, medication compliance, and socioeconomic variables (Carson et al., 2014; Li et al., 2005; Marino et al., 2016; Stubbs et al., 2024).

The present study sought to determine whether psychiatric …

Exploring The Diversity And Biology Of Unique Giant Broad Host Range Bacteriophages In Natural Environments, Tannaz Mohammadi

Theses and Dissertations

Microbial interactions within the rhizosphere play a crucial role in shaping bacterial community diversity and function. To explore these dynamics, we isolated 21 bacterial strains from soil and plant root samples collected near Rocky Branch Creek on the University of South Carolina campus. Our findings revealed that a significant proportion of these bacteria are lysogenic for CbK-like phages belonging to the Dolichocephalovirinae subfamily. Additionally, we isolated free bacteriophages directly from the same soil samples and found them to be closely related to those harbored within lysogenic bacteria. Surprisingly, despite predictions of a narrow host range, most of these phages, both …

Unveiling Caulocovert: Conserved Phage And Gene Transfer Agent In Caulobacter, Talia Neco

Theses and Dissertations

Bacteriophage play an important role in bacterial evolution, population control, and horizontal gene transfer within microbial ecosystems. This study focuses on the discovery and characterization of Caulocovert, an active bacteriophage identified in multiple Caulobacter strains. Using genome annotation tools such as Artemis and Mauve, we identified multiple key phage genes, including the major capsid protein, head-tail connector, portal protein, and major tail protein. These phage genes are present in CB13 and several other strains, such as C. vibriodies CB15 and CB2, C. segnis CBR1, Caulobacter sp. FWC26, and the distantly related Caulobacter sp. D5 strain. The location of these genes …

The Role Of Prelimbic Projections To The Rostromedial Tegmnetal Nucleus In Cue Discrimination, Emma Lane Carlson

Theses and Dissertations

Maladaptive decision-making, such as persistent reward seeking despite negative consequences, is a hallmark of substance use disorder (SUD) and related psychiatric conditions. These behaviors are thought to arise from impairments in aversive learning and disrupted neural signaling that normally supports behavioral inhibition. The prelimbic cortex (PL) plays a critical role in encoding cue–outcome associations and exerting top-down control over behavior, while the rostromedial tegmental nucleus (RMTg) serves as a GABAergic inhibitor of midbrain dopamine neurons in response to aversive stimuli. Although both structures have been implicated in reward and avoidance processes, little is known about how direct PL-RMTg projections contribute …

Enhancing Teacher Self-Efficacy In Ngss Storyline Implementation: A Mixed Methods Action Research Study On Professional Development In Middle School Science Education, Dena Spickard Fender

Theses and Dissertations

This mixed-methods action research study examined how professional development (PD) influences middle school science teachers’ self-efficacy in implementing the Next Generation Science Standards (NGSS) Storyline curriculum. The study also explored which aspects of PD are most effective in supporting teachers at different career stages. Teacher self-efficacy is a critical factor in the successful adoption of reform-based science instruction, yet limited research has investigated how PD can be differentiated to address the needs of novice, intermediate, and veteran educators in underperforming schools.

A convergent mixed-methods design was employed to collect both quantitative and qualitative data. The Science Teaching Efficacy Belief Instrument-A …

The Processing Of Emotional Prosody And Semantics In Native And Non-Native Chinese Speakers, Cheng Xiao

Theses and Dissertations

Emotional information in spoken language is conveyed through both semantic and prosodic cues. While listeners interpret the meaning of utterances (what is said), they also detect the speaker’s emotional prosody (how it is said). For non-native speakers, processing these two types of information simultaneously can be challenging, particularly in a tonal language such as Mandarin Chinese, where the same acoustic feature (i.e., pitch) encodes both lexical semantics and emotional prosody. This dissertation reports a series of behavioral and fMRI experiments investigating how native and non-native Chinese speakers perceive and process emotional prosody and semantics in words and sentences. Behaviorally, results …

Improving District Targeted Support For Schools With Low Student Achievement By Implementing Assistance Relationships With School Leaders, Mary Ashley Gaskins

Theses and Dissertations

Riverbend School District faced a challenge: the lack of a formalized structure for providing schools with targeted support that meets their unique needs and increases student achievement. This study explored how the district-level instructional team could customize support to improve English Language Arts and Math performance at schools with low student achievement. The theory of improvement posited that district instructional leaders engaging in assistance relationships – through modeling instructional leadership and providing conceptual and practical tools – would enhance school leaders' capacity, ultimately improving teaching and learning. A key action of the improvement team was the creation and implementation of …

Anthropomorphism In Language And Thought: The Effect Of Agentive Linguistic Framing On Readers’ Perceptions Of Ai Animacy, Dawson Petersen

Theses and Dissertations

Over the past decade, deep learning AI systems have gone from academic obscurity to near cultural centrality in the developed world. As these technologies become increasingly integrated into daily life, there is growing cause for concern about AI anthropomorphism—i.e., the perception of AIs as more human than they really are. Despite the potential risks, AI developers continue to market their technologies using agentive language that seems to be designed to encourage anthropomorphism. The current dissertation utilizes behavioral and eye-tracking methods to examine the relationship between agentive linguistic framing and AI anthropomorphism in both controlled judgments and automatic inferences. The results …

Behind The Gens: A Bioarchaeological Study Of Kinship Structure And Social Change In Iron Age Latium, Italy, Andrea Noemí Acosta Caballero

Theses and Dissertations

The cemetery of Osteria dell’Osa is the only completely published Iron Age cemetery from Latium. Its research legacy continues as dietary, mobility, and biological affinity patterns were reconstructed through carbon, nitrogen, oxygen, and strontium isotope analyses and biodistance analysis using bone and dental samples for this dissertation project. The main hypotheses tested are:

a) The diet of people buried at Osteria dell’Osa during the Iron Age likely consisted of mostly grains like wheat and barley, which may yield low carbon isotope signatures;

b) The protein consumption of the diet of people at Osteria dell’Osa was mainly composed of terrestrial protein …

Food Insecurity And Social Connection Of Nicaraguan Refugees And Asylum Seekers In Costa Rica: A Qualitative Study, Keren Herran

Theses and Dissertations

Background

Given the physical and mental health consequences of food insecurity, solutions should be evidence-based and seek to prioritize vulnerable populations. This qualitative study adds to limited literature on how refugees and asylum seekers use their social connections to mitigate food insecurity by investigating how Nicaraguan refugees and asylum seekers in Costa Rica foster social connections and use these connections to acquire and manage food.

Methods

In fall 2024, 35 in-depth semi-structured interviews were conducted in San José, Costa Rica. Participants were recruited through six migrant-serving nonprofit organizations. For study inclusion, participants had to: 1) be 18 or older; 2) …