Digital Commons Network^™

Effect Of Short-Term Exposure To Pesticide Mixture On Tissue Architecture, Body Fluid Conditions, Nitrative Stress And Osmoregulation Of American Oyster, Crassostrea Virginica, Asif Ahmed

Theses and Dissertations

Pesticides are used widely to control weeds and pests in agricultural settings but harm non-target aquatic organisms. We examined the effect of short-term exposure (one week) to pesticide mixtures (low dose: 0.5 μg/L Roundup®, 0.5 μg/L 2,4-D, and 0.4 μg/L atrazine; high dose: 1 μg/L Roundup®, 1 μg/L 2,4-D, and 0.8 μg/L atrazine) on American oyster (Crassostrea virginica, an important shellfish and seafood species) under controlled laboratory conditions. Histological analyses demonstrated the atrophy in oysters' gills, digestive glands, and connective tissue. The protein concentrations and glucose levels dropped significantly in the high-dose treatment. The number of hemocytes in connective tissue …

Pipecolic Acid And Novel Insights Into Cerebral Malaria, Akua E. Mensah

Theses and Dissertations

Cerebral malaria (CM), a severe manifestation of Plasmodium infection, prompts our investigation into the nuanced role of pipecolic acid in its pathophysiology. To unravel the molecular intricacies, we conducted in vitro lysine labeling techniques of mice infected with P. berghei ANKA parasites, and human P. falciparum grown in vitro, aiming to discern the impact of Plasmodium on pipecolic acid production. Previous observations indicated an elevation in pipecolic acid levels correlating with neurological decline in children with CM. In our study, confirming elevated pipecolic acid presence in the plasma and brain tissues of CM patients and the animal model of CM, …

Bayesian Estimation Of Reproduction Numbers From Distributions Of Outbreaks Sizes: Branching Process Approach, Alberta Araba Johnson

Theses and Dissertations

The Generalized Poisson distribution is useful in modeling epidemiological processes as a branching stochastic processes problem. Our goal is to construct accurate and reliable estimators for the reproduction number (R0) (i.e., the number of secondary infections), particularly in the context of disease outbreaks modeled by a Galton-Watson process. Towards this goal, we construct the classical Bayes estimator, the Maximum Likelihood estimator, and the Empirical Bayes (EB) estimator under the Square Error Loss function in Chapter II. We prove that the Empirical Bayes estimator is asymptotically optimal and estimate the rate of convergence. We then proceed to monotonize the Empirical Bayes …

Navigating School Restructuring In The Post-Covid Era: A Principal’S Perspective In Sustaining Organizational Health While Bridging The Academic Achievement Gap, Alma Alicia Perez-Campos

Theses and Dissertations

In 2019, the COVID-19 pandemic led to nationwide school closures, followed by a mandate for schools to reopen in 2020, posing challenges for ensuring both educational continuity and student safety. This paper presents findings from a mixed-method study involving ten campus principals in District A, South Texas, post-pandemic. Qualitatively, the study explored the principals' perspectives on restructuring their campuses to address achievement gaps while maintaining organizational health. Quantitatively, it examined the relationship between achievement and organizational health scores and any differences in organizational health pre- and post-COVID-19. The study aimed to offer insights for future …

To Decipher Genomic Evolution Of Dengue Virus And To Probe The Activation Mechanism Of A Her2 Directed Chimeric Antigen Receptor Toward Computer Aided Drug Design (Cadd), Mariya Hryb

Theses and Dissertations

This study encompasses three significant chapters focusing on diverse yet interconnected aspects of pharmaceutical research. Chapter 1 delves into the complexities of drug development, emphasizing the challenges and the pressing need for advanced technologies to streamline the process. Computational methods like Computer-Aided Drug Design (CADD) extend their scope beyond small molecules, aiding in the design of intricate biomolecules vital for biomedical advancements, especially in immunotherapy. Chapter 2 introduces the Near-Neutral Balanced Selectionist Theory (NNBST) validated through analysis of Dengue virus evolution. The unique features observed align with NNBST, surpassing existing theories. This approach offers a deeper understanding of viral evolution …

The National Basketball Association Communications Strategy For The 2019-20 Season Restart, Trevor Dale Jones

Theses and Dissertations

This paper analyzes the NBA's communications efforts from June 4 to July 30, 2020 as it prepared for an unprecedented season restart in the early days of a global pandemic. While scholars have examined the media's framing of the NBA in this period, there is a gap in the literature when looking at the official NBA communications strategy of the organization itself. As the first study to use risk communication theory in a sports scenario while also employing corporate social advocacy as the second theoretical basis of analysis, this qualitative study is a thematic and textual analysis of 14 NBA …

Drug Modification Of Synaptic Plasticity At The Inhibitory Synapses Involving Gaba Neurons In The Ventral Tegmental Area, Jie Wu

Theses and Dissertations

The neurobiological underpinnings of substance use disorders (SUDs) involve complex alterations in synaptic plasticity within the mesolimbic dopamine system, particularly within the ventral tegmental area (VTA). While dopamine neurons in the VTA are extensively studied in addiction research, less attention has been given to the plasticity exhibited by VTA GABAergic neurons, despite their established regulatory role in modulating dopamine cell activity. Building upon the ground-breaking work of Bocklisch et al. (2013), we investigated the plasticity at the GABAergic to VTA GABA neuron (VTA GABA-to-GABA) synapse utilizing whole-cell electrophysiology in GAD67 GFP knock-in mice. Our findings revealed an exceptionally rare duo-plasticity …

Mothers With Disabilities As Health Navigators: Narratives Of Pregnancy, Postpartum Depression, And Parenting, Agnes Amoakwa

Theses and Dissertations

Scholars have focused on factors that affect pregnancy decisions, linking it to internal factors such as physical attachment and love towards children and feasibility of external factors, among others, while others have focused on experience with health professionals and accessibility of healthcare facilities and equipment during access to maternal care. While these studies are important, fewer focus has been placed on how women with disabilities navigate the postpartum phase of motherhood and the support needed to navigate through this experience. I employed the communicated narrative sense-making theory to explore the meanings of stories shared by mothers with disabilities about their …

Preventing Orthopedic Implant Associated Biofilm Infections Using Transition Metal Complex Laminated Bioactive Implants, Sowndarya J

Theses and Dissertations

Orthopedic implants are medical devices employed to repair the damaged structure and function of an injured musculoskeletal system. It helps millions of people suffering from bone fractures and arthroplasty. The increased use of orthopedic implants has given rise to implant-associated biofilm infections. Biofilm-mediated Implant-associated osteomyelitis (IAO) is a major concern in the medical field, wherein the treatment involves debridement, administration of antibiotics and removal of implants leading to increased cost of treatment.

The keystone bacterial pathogens involved in orthopedic implant-associated infections are Staphylococcus aureus and Acinetobacter baumannii which are members of the ESKAPE pathogens. A. baumannii has gained resistance against …

Utilization Of The System Engineering Design Process To Design And Test A Low-Cost Infectious Aerosol Control Mechanism For Patient Aeromedical Evacuation, Sara Shaghaghi

Theses and Dissertations

The aeromedical evacuation of military patients is a critical component of care for Armed Forces members. The Air Force’s ability to transport patients relies on the technology and systems available. A vital transport responsibility is keeping the patient and medical personnel safe during transport. The historical and legacy systems provide reliable transport mechanisms for the Armed Forces’ patients infected with high-level biological agents, but drawbacks must be considered. This dissertation will discuss the development, conceptual design, and initial evaluation of a new low-cost, litter-mounted patient transport system, Biological-Mitigation in Patient Transport (B-MIPT), using the “V” model of the system engineering …

An Introduction Of Adaptive Training Aid Concepts And Its Application To Accelerated Training For Air Battle Managers, John C. Gillispie

Theses and Dissertations

Over the years, the integration of artificial intelligence (AI) to enable autonomous systems has undergone transformative shifts in the Department of Defense (DoD), revolutionizing capabilities and strategic approaches. To further optimize these advancements, varying levels of autonomy have been introduced across critical military applications, spanning intelligence, surveillance, reconnaissance (ISR), air battle management, and offensive/defensive air operations. As the technological landscape expands, so do the opportunities for autonomy to augment operations through human-agent teaming. Within the Air Force, one notably cognitively demanding role that stands to benefit from these strides is that of the Air Battle Manager (ABM). In support of …

Deploying Field-Capable Genome Sequencing: Human Performance Considerations Under Various Personal Protective Equipment Ensembles, Steven A. Torres

Theses and Dissertations

Field-portable genome sequencing enhances the Department of Defense's strategy for detecting biological threats, particularly in resource-limited environments. While current field-capable detection methods are effective, challenges in identifying emerging threats pose risks to personnel safety. As biotechnological advancements like portable nanopore genome sequencing can advance biological detection capabilities, the viability of conducting laboratory tasks under Mission-Oriented Protective Posture (MOPP) ensembles lacks sufficient data. This research assesses the effectiveness of tools like pipettes, centrifuges, vortexes, and nanopore sequencing within a MOPP ensemble. Novice participants were utilized to explore the extension of this capability, and they completed tasks in three conditions: Fatigues, Laboratory …

Integrative Genomic Insights Into Coral Resilience: Adaptive And Acclimatory Responses To Seasonal Environmental Shifts, Tasnim Ghanim

Theses and Dissertations

Coral reefs, critical to marine biodiversity and coastal protection, face escalating threats from climate change-related phenomena such as ocean warming. This warming is leading to widespread thermal stress that contributes to coral bleaching and infectious disease in corals, leading to the disruption of marine ecosystems and the economies that depend on them. Focusing on the brain coral Platygyra daedalea, known for its thermal resilience in the Persian Arabian Gulf—a region that exemplifies the extreme stressors of climate change—our study aims to dissect the genetic, transcriptomic, and microbiological underpinnings of coral survival in high-temperature environments predicted for the end of …

Ameliorating School Experience With Restorative Practices, Faith Thompson-Lee

Theses and Dissertations

The purpose of this quantitative, non-experimental study was to explore the association between school success after the tier-one introduction of a three-tiered restorative practices program. To understand school success, student factors were examined using middle school data from a suburban school district located outside of a large metropolitan city in the northeastern part of the United States. These factors included achievement (Final GPA), attendance (Absences), and suspensions (In-school and Out-of-school) across racial groups. Separate multiple regressions were conducted for each school success factor on racial group for the 2021-2022 and the 2022-2023 school years. By this time, all district staff …

Teachers’ Perspective On Social-Emotional Learning Through The Responsive Classroom Approach For Elementary Students During A Traumatic Event, Sheena Burke

Theses and Dissertations

Social-emotional learning (SEL) has become a significant concern for schools across the United States and abroad, especially during the COVID-19 pandemic. Research suggests that school support is necessary for students to succeed academically and emotionally. In recognition of this need, the New York State Education Department mandated schools to implement a SEL program that focuses on developing self-awareness, self-management, social awareness, relationships, and responsible decision-making skills. Many SEL programs have been researched to determine their effectiveness in affecting students’ overall well-being. This phenomenological study used interviews with K–6 Responsive Classroom teachers in the Tri-State Area to determine the benefits of …

Educators’ Perceptions And Experiences Of Teachers On Safety Guidelines And Protocol Adherence For In-Person Teaching During The Covid-19 Pandemic, Maria Paros

Theses and Dissertations

The coronavirus disease 2019 (COVID-19) pandemic has had a devastating and long-lasting effect on most of the world. In an attempt to slow the spread of the virus, public health authorities instituted guidelines for schools regarding social distancing, mask wearing, and quarantining, and the U.S. educational system was forced to adjust. This phenomenological study examines, from the perception and experiences of primary school teachers, how teachers returned to in-person education while adhering to CDC guidelines and regulations during the COVID-19 pandemic. Fifteen experienced primary teachers in three public school districts across Long Island, New York were involved in the study …

Coping With The Covid-19 Pandemic: Examining Meaning-Making In A Socioecological Framework, Emilia Eva Mikrut

Theses and Dissertations

During 2020, the COVID-19 pandemic emerged as a threatening, unpredictable, and uncontrollable stressor. Meaning-making, or one’s ability to make sense of a stressful life event, integrate the event into one’s narrative of the world and meaning in life, and accordingly revise life goals, is a salient intrapsychic process contributing to psychological adjustment in the face of very stressful or traumatic experiences such as chronic health issues, interpersonal grief, and natural and man-made disasters. Early findings provide evidence for the critical role of meaning-making in coping with stressors associated with the COVID-19 pandemic. Though meaning-making is a universal process, one’s capacity …

Production Of Vp3-Only Aav2 Vlp In E. Coli, Chengyu Fu

Theses and Dissertations

Adeno-associated virus (AAV) is a widely used vector for gene therapy. However, few studies have focused on producing AAV-based virus-like particles (VLP) in E. coli. This project aimed to express VP3 of AAV2 or co-express VP3 of AAV2 with other proteins, such as E. coli chaperones and/or assembly-activating protein (AAP) in E. coli under different expression conditions and explore the potential to produce VP3-only VLP of AAV2 in situ in E. coli. The constructed plasmid pET15ΔHis-VP3 was used to express VP3 under different conditions. The results demonstrated that most expressed VP3 was in inclusion bodies in all tested conditions. Although …

Teacher Perceptions Of Their Preparedness To Teach Early Literacy Skills Using Developmentally Appropriate Practices In Universal Prekindergarten Programs, Tara A. Sokol

Theses and Dissertations

Early childhood education is a dynamic field, constantly evolving with the introduction of new curriculums, initiatives and programs. The classroom teacher is the constant, providing essential early literacy instruction amidst all of these changes. Universal Pre-Kindergarten is a vital program that provides access to quality early childhood education for New York City residents. According to the Office of Early Childhood Education, there were nearly 68,000 students enrolled in these free, full-day, high-quality programs in the 2018-2019 school year (2022). Universal Pre-Kindergarten is described as using Developmentally Appropriate Practice (DAP) as the basis for its teachings. Educators are responsible for using …

Exploring The Influence Of Culturally Relevant Texts On Black Male College Students’ Literacy Practices: A Case Study, Beverly A. Fields

Theses and Dissertations

African American males often experience literacy challenges in the American school system. Texts related to their lives are rarely available in the curriculum, and some educators need the skillset or strategies to engage these learners with texts related to their lives. Many studies have documented that exposure to culturally relevant texts may enhance African American males’ literacy practices. This qualitative case study explored the following research question: How do culturally relevant texts influence college-age African American males’ literacy practices? Data were collected from the instructor and student interviews, field notes, and document analysis. Seven African American college-age students were recruited …

Black Males’ Experiences Of Resilience: A Narrative Study On College Students Experiences During The Covid-19 Pandemic, Keshia R. Graham

Theses and Dissertations

Black men’s resilience has historically been discussed, interwoven, and disguised by deficit-based models rather than strength-based ones. The current study examined Black men's resilience using a strength-based model for Black male students in higher education institutions. Specifically, the experiences and resilience of Black men before and during the COVID-19 pandemic were explored. This study targeted Black male students from an urban institution, with the aim to find out what key elements supported their resilient behavior throughout the most challenging times in their college career. The African American Male Theory and Resiliency Theory used as the theoretical framework. A narrative methodology …

Sphingosine Kinase 2 Depletion Dysregulates Hepatic Redox Metabolism And Enhances Inflammation In Early Metabolic Dysfunction-Associated Steatohepatitis (Mash), Kaitlyn Georgene Jackson

Theses and Dissertations

Metabolic dysfunction-associated steatotic liver disease (MASLD) currently affects one in three people worldwide; its prevalence is growing alongside other cardiometabolic disorders. Aberrations to redox and sphingolipid metabolism contribute to MASLD pathologic progression. Sphingosine-1-phosphate (S1P) is an active lipid, meaning that S1P participates in biochemical processes within the cell. S1P is generated by two distinct isoenzymes, sphingosine kinase (SphK)1 and SphK2. The localization of the SphK enzyme dictates S1P function. While cytosolic SphK1-generated S1P is well-characterized, nuclear and mitochondrial SphK2-generated S1P is less known. Moreover, SphK2 depletion effects on cellular redox processes and inflammation are not well-defined.

This study defines the …

The Role Of Hdac6 In Periodontal Inflammation, Hannah S. Lohner

Theses and Dissertations

Periodontal disease is a polymicrobial infection-induced chronic inflammatory disease and results in irreversible tissue damage and bone loss. While bacteria play a large role in the initiation of this disease, excessive and prolonged inflammatory immune responses are fundamental for its progression, making host directed therapy (HDT) a possible and potentially valuable treatment. Histone Deacetylase 6 (HDAC6) is an enzyme that removes acetyl groups from both histone and non-histone proteins, giving it a potential role in regulating inflammation initiated by pathogenic oral bacteria. Previous studies indicated that HDACs act as a bivalent regulator in inflammatory responses; however, the role that HDAC6 …

A Bioarchaeological Overview Of The Late Woodland Period Gooden Mound Group, Fulton County, Illinois, Aaron M. Durchholz

Theses and Dissertations

Extensive research in paleopathology has documented adverse effects accompanying a shift in subsistence strategy from largely nomadic, hunting and gathering lifestyles to those of more sedentary, agrarian systems. Comparative assessment of the human skeletal remains of early foragers and later farmers recovered from archaeological sites around the world have consistently demonstrated a decrease in average age at death in the latter.

Across the pre-Columbian North American Midwest, the transitional period between the Late Woodland and Mississippian periods, sometimes referred to as the terminal or Mississippian Acculturated Late Woodland, portended establishment of semi-permanent to permanent hamlets, increasingly reliant upon the adoption …

A Scoping Review Of Perceptions And Fears Experienced By Families With Children With Developmental Disabilities In Sub-Saharan Africa., Isaac Twum Boamah

Theses and Dissertations

The prevalence of perception and fear surrounding families with children with developmental disabilities in Sub-Saharan Africa reveal a complex interplay of cultural, social, economic, and healthcare factors. This interplay of factors results in stigma, which often leads to societal misconceptions and discrimination, exacerbating the challenges faced by affected families. This study seeks to identify the individual and family perception of developmental disabilities, document the stigma experienced by the individual and their families, and document the resources available to help alleviate the plight faced by the individuals and their families. It further seeks to find gaps in the literature on developmental …

A Quality-By-Design Approach For The Development Of Inhalable Dry Powders For Tuberculosis (Tb) Treatment, Suyash M. Patil

Theses and Dissertations

Tuberculosis (TB), caused by Mycobacterium tuberculosis (M.tb), remains a pressing concern globally. The emergence of drug-resistant strains and the need for prolonged treatment regimens continue to complicate disease management. Innovative therapeutic strategies are urgently required to enhance treatment efficacy, improve patient adherence, and minimize adverse effects. Toward this, strategies involving use of drug combinations, reducing dosing frequencies, and targeting TB-infected cells are gaining attention. In my dissertation, we explored the combination therapy involving two FDA-approved anti-TB drugs, Bedaquiline (BDQ) and Pretomanid (PTD). The BDQ-PTD combination reduced minimum inhibitory concentration (MIC), compared to individual drugs and demonstrated additive/synergistic effects at 1:4 …

Business Managers’ Adaption Of Communication And Leadership Characteristics Amidst Covid-19: A Qualitative Study, Shadan Shamloo

Theses and Dissertations

The problem being studied was that because managers of for-profit businesses in California were having to communicate with their employees outside of face-to-face internal interactions (e.g., Zoom conferencing and telephone). It was unknown how business managers of for-profit businesses in California had adapted their communication and leadership styles when responding to COVID-19’s social distancing guidelines. The purpose of this qualitative descriptive study was to explore how managers of for-profit businesses in California described how their communication and leadership styles had been adapted to follow the social distancing guidelines of COVID-19. This study was guided by the communication accommodation theory and …

Exploring Strategies For Social Impact In Cross-Sector Partnerships: A Multiple Case Study Approach, Melanie Venture

Theses and Dissertations

The UN’s Sustainable Development Goals (SDGs) identify critical society challenges in areas of poverty, gender and income equality, health, and well-being. Consensus exists among scholars and practitioners on the need for cross-sector partnerships (CSPs) for achieving the SDGs. However, we have yet to identify the means of fostering collaboration to yield optimal social impact while also generating measurable results and financial returns. We lack actionable strategic frameworks for explaining how CSPs should be implemented. This multiple case study examined five socially oriented business-nonprofit partnerships and addressed how collaboration strategies can increase social impact of CSPs. The findings revealed important insights …

Faculty Support And Resource Seeking, A Collective Experience Amid The Covid-19 Pandemic: A Retrospective, Amber Victoria Stokes

Theses and Dissertations

This study aimed to capture collective faculty experiences of adapting to teaching entirely or partially online during the three years of the COVID-19 pandemic. The literature suggests that emergency remote teaching (ERT) was only a temporary stopgap for higher education institutions and faculty to move instruction online early in the pandemic (Hodges et al., 2021). Before the initial move to being entirely online in March 2020, higher education institutions had already lagged compared to other industries in providing digitally driven, outcomes-focused business models (Gallagher & Palmer, 2020). Therefore, it is no surprise that higher education leadership, faculty, and staff faced …

Preexisting Conditionings: Painting, Gaming And Narrative Play, Pia Bakala

Theses and Dissertations

My work incorporates play, fantasy, and self-actualization through painting, sculpture, and video. In this essay, I will explore the ways in which my studio practice intersects with topics across fields of visual arts and in conjunction their theoretical frameworks. I will discuss the culminating body of work, tentatively titled Preexisting Conditionings, that my academic experience at VCU has fostered and trace its development across specific precursive works, as well as the cultural and intellectual touchstones influencing its creative impetus. In Preexisting Conditionings, I work primarily use oil painting, creating mostly representational figurative tableaus that correspond to narratives and characters I’m …