Digital Commons Network^™

Purification And Biological Activity Of Serine Lipids Of Porphyromonas Endodontalis, Joseph M. Blondin

Master's Theses

Porphymonas endodontalis has been shown to populate necrotic root canal systems and may contribute to apical bone loss. Recent work has shown that P. endodontalis synthesizes a novel serine lipid class (Lipid 654) similar to that produced by P. gingivalis. Objective: The purpose of this study was to isolate P. endodontalis Lipid 654 in very high purity and characterize its structure and biological activity
 in RAW 264.7 cells and HEK293 cells. Methods: P. endodontalis (ATCC 35406, type strain) was grown under anaerobic conditions and centrifuged. P. endodontalis total lipids were recovered using the Bligh and Dyer phospholipid extraction procedure and …

The Role Of Calcium Transients In The Collective Migration Of Goldfish Epithelial Keratocytes, Chelsea E. Willet

Master's Theses

Collective migration is a critical biological process that involves the coordinated migration of a group of cells connected via adherens junctions. These cell-cell adhesions are regulated by intracellular and extracellular calcium, and require controlled turnover in order to maintain the structural integrity of migrating cell sheets. In single fish keratocytes, intracellular calcium signaling via stretch-activated calcium channels facilitates migration by regulating cytoskeletal contractility and cell-substratum adhesion turnover. The role of calcium signaling in collectively migrating keratocyte sheets remains unknown. In this study, we investigate the effects of modulating the frequency of calcium transients in goldfish keratocyte sheets, using EGTA and …

Developent Of A Phospholipid Encapsulation Process For Quantum Dots To Be Used In Biologic Applications, Logan Grimes

Master's Theses

The American Cancer Society predicts that 1,665,540 people will be diagnosed with cancer, and 585,720 people will die from cancer in 2014. One of the most common types of cancer in the United States is skin cancer. Melanoma alone is predicted to account for 10,000 of the cancer related deaths in 2014. As a highly mobile and aggressive form of cancer, melanoma is difficult to fight once it has metastasized through the body. Early detection in such varieties of cancer is critical in improving survival rates in afflicted patients. Present methods of detection rely on visual examination of suspicious regions …

Draft Environmental Impact Report: City Of Clearlake General Plan Update, Hannah Cha

Master's Theses

The City of Clearlake in northern California initiated its first general plan update in 2012.The City decided to do an Environmental Impact Report (EIR) for the general plan update in order to fulfill the California Environmental Quality Act’s (CEQA) requirements.The author wrote the Agriculture and Biological Resources sections of the EIR.She explains the CEQA process for a programmatic-level EIR, and summarizes the lessons learned and recommendations for CEQA.

General CEQA issues include fear of litigation and vague requirements for thresholds of significance.Additional CEQA issues include difficulty applying the same level of analysis to programmatic projects when the Environmental Checklist is …

The Effect Of Temperature And Extraction Technique On The Binding Interactions And Hydrolysis Of Β-Lactoglobulin With Milk Fat Globule Membrane (Mfgm), Corbin R. Kembel

Master's Theses

Whey protein, containing predominately b-Lactoglobulin (b-LG), is a popular choice among consumers looking for an excellent protein source. Likewise, fat is a natural component in milk and dairy products. Lipids are packaged within a membrane called the Milk Fat Globule Membrane (MFGM). The MGFM contains a variety of lipids and proteins. Although b-LG has been extensively characterized, the function of the protein is largely unknown. The objectives of this study were to assess the enzymatics of b-LG in an isolated system, evaluate the propensity of b-LG to bind to MFGM, determine the effects of temperature and fat extraction method on …

Beef Export Price Response To Sanitary Status And Traceability Systems: Implications For Paraguay, Silvana Careaga

Master's Theses

Global beef markets are highly influenced by sanitary issues, mainly related to animal health and food safety, which have been determining levels and trends in global beef trade. Food safety issues affecting human health such as bovine spongiform encephalopathy (BSE) associated with Creutzfeldt-Jacob Disease (vCJD) in humans, and other diseases affecting production such as foot and mouth disease (FMD) has encouraged putting these issues to beef trade attention. A way to address these concerns and comply with international standards is the use of traceability systems in order to quickly identify hazard sources and assure beef quality and safety. The goal …

Assessing Rodent Species Counts And Diversity In The Not-Grazed Montana De Oro State Park And The Rotationally Grazed Pecho Ranch, Nancy Marie Lemos

Master's Theses

The available data examining the influence of rotational grazing on rodent responses are limited. This study investigated how rotational livestock grazing practices influence small mammal rodent abundances and species diversity. We looked for evidence of variation in the occurrence and/or numbers of certain rodent species among three plant community types (grassland, shrubland, grass-shrub mix), managed with or without grazing. We used Sherman live traps over a total of eight trapping sessions. We totaled 486 trapnights for each of the six plant community and grazing management combinations. The three plant community types were identified by visual cover.

Out of the eight …

Effects Of Ovariectomy And Anatomical Position On The Compact Bone As Seen In Three Month Summer Ovine Model, Bryan C. Kraft

Master's Theses

The purpose of this study is to characterize the compact-bone remodeling response to an ovariectomy model of human postmenopausal osteoporosis. Animal models are a beneficial practice allowing for the evaluation of the effectiveness of medical therapies and devices on diseases. The ovine model was chosen for this study due to its large size, similar bone remodeling to humans, and cost effectiveness. The main obstacle to overcome with the use of the ovine model was the lack of natural menopause experienced by this species. To overcome this, it was necessary to perform an ovariectomy to create estrogen depletion and artificially induce …

An Investigation Of Poly(N-Isopropylacrylamide) For Applications With Microfluidic Paper-Based Analytical Devices, Haydn Thomas Mitchell

Master's Theses

N,N′-methylenebisacrylamide-crosslinked poly(N-isopropylacrylamide), also known as P(NIPAM), was developed as a fluid delivery system for use with microfluidic paper-based analytical devices (microPADs). MicroPADs are postage-stamp-sized devices made out of paper that can be used as platforms for low-cost, simple-to-use point-of-care diagnostic assays. P(NIPAM) is a thermally responsive polymer that absorbs aqueous solutions at room temperature and will expel the solutions to microPADs when heated. The fluid delivery characteristics of P(NIPAM) were assessed, and P(NIPAM) was able to deliver multiple solutions to microPADs in specific sequences or simultaneously in a laminar-flow configuration. P(NIPAM) was then shown to be suitable …

Cayucos Community Health Plan, Jennifer Joyce Franich

Master's Theses

Recent, mounting research shows that chronic disease, the leading causes of death and primary driver of health care costs, cannot be effectively addressed through education or preventative health alone. A physical environment that promotes health—through access to healthy food, opportunities for physical activity, quality housing, transportation options, and safe schools—is an integral part of making our communities healthier. This research and accompanying Healthy Community Plans will serve as a way for the County to begin looking in-depth at the ways the built environment (our streets, parks, and neighborhoods) contribute or detract from the health of the community. Though the creation …

Water And Nutrient Recycling In High Rate Algae Ponds Fed In Primary Treated Municipal Wastewater, Michael Field Chang

Master's Theses

Algal biofuels present a promising future alternative to petroleum based fuels. Water nutrient recycling is a key step to increase the sustainability of algae biofuel production facilities. This thesis discusses the process of nutrient and water recycling in high rate algae raceway ponds (HRAP) fed primary treated municipal wastewater. Research was conducted primarily at the San Luis Obispo Water Resource Reclamation Facility (SLOWRRF). Nine 30 m², 0.3 m deep HRAP’s were operated continuously from June 1, 2013 to April 17, 2014. The ponds were arranged in three sets of triplicate ponds, with two pond sets run on 3-day …

University Square Development Proposal, Tom Gregory Van Pelt

Master's Theses

The University Square Development Proposal (USDP) explores the redevelopment of the underutilized University Square site (the Site) in the City of San Luis Obispo (the City.) The Sites proximity to California Polytechnic State University, San Luis Obispo (Cal Poly), a university with significant student housing needs, makes it an ideal location for student housing. The City has also expressed interest in the Site, having identified it in the General Plan Land Use and Circulation Element (LUCE) update as a “Special Planning Area”. The LUCE proposes a new mixed-use typology on the Site that may include a mixture of multi-family housing, …

Juiciness In Citizen Science Computer Games: Analysis Of A Prototypical Game, Eric D. Ebucktha Buckthal

Master's Theses

Incorporating the collective problem-solving skills of non-experts could ac- celerate the advancement of scientific research. Citizen science games leverage puzzles to present computationally difficult problems to players. Such games typ- ically map the scientific problem to game mechanics and visual feed-back helps players improve their solutions. Like games for entertainment, citizen science games intend to capture and retain player attention. “Juicy” game design refers to augmented visual feedback systems that give a game personality without modi- fying fundamental game mechanics. A “juicy” game feels alive and polished. This thesis explores the use of “juicy” game design applied to the citizen …

Characterizing The Reproducibility Of The Properties Of Electrospun Poly(D, L-Lactide-Co-Glycolide) Scaffolds For Tissue-Engineered Blood Vessel Mimics, Toni M. Pipes

Master's Theses

“Blood vessel mimics” (BVMs) are tissue-engineered constructs that serve as in vitro preclinical testing models for intravascular devices. The Cal Poly Tissue Engineering lab specifically uses BVMs to test the cellular response to stent implantation. PLGA scaffolds are electrospun in-house using the current “Standard Protocol” and used as the framework for these constructs. The performance of BVMs greatly depends on material and mechanical properties of the scaffolds. It is desirable to create BVMs with reproducible properties so that they can be consistent models that ultimately generate more reliable results for intravascular device testing. Reproducibility stems from the consistency of the …

Optimization Of Growth Parameters For Algal Regrowth Potential Experiments, Chadwick D. Boggess

Master's Theses

Water cost is one of the key deterrents in making algal biofuels a feasible alternative to traditional petroleum fuels. Research into recycling of algal media was conducted in a laboratory setting at the California Polytechnic State University in San Luis Obispo. A growth assay was created to allow for algal cultures, specifically Ward’s Chlorella sp., to proliferate in sealed culture vessels. Bold’s Basal Medium (BBM) was selected as the media due to its extensive use with freshwater algal strains. However, this medium can limit algal growth through nutrient limitation and it became necessary to augment the original recipe. 50mM …

Stabilization Of Horseradish Peroxidase Using Epoxy Novolac Resins For Applications With Microfluidic Paper-Based Analytical Devices, Cory A. Chaplan

Master's Theses

Microfluidic paper-based analytical devices (microPADs) are an emerging platform for point-of-care diagnostic tests for use by untrained users with potential applications in healthcare, environmental monitoring, and food safety. These devices can be developed for a multitude of different tests, many of which employ enzymes as catalysts. Without specialized treatment, some enzymes tend to lose their activity when stored on microPADs within 48 hours, which is a major hurdle for taking these types of devices out of the laboratory and into the real world. This work focused on the development of simple methods for stabilizing enzymes by applying polymers to chromatography …

The Effect Of Pretreatment Methods On Methane Yield And Nutrient Solubilization During Anaerobic Digestion Of Microalgae, Alexander Scott Hill

Master's Theses

Microalgal biomass is a candidate feedstock for biofuel production. To improve the sustainability of algae biofuel production, following biofuel recovery, the biomass nutrients should be recycled for additional algae growth. Anaerobic digestion of algae or oil-extracted algae is a means of recovering carbon and other nutrients, while offsetting algae production electricity demand. The major limiting factor in microalgae digestion is the low biodegradability of the cell walls. In the present study, various pretreatment technologies were tested at bench scale for their ability to improve raw, non-lipid-extracted algae biodegradability, which was assessed in terms of methane yield, volatile solids destruction, and …

Investigation Of Chromosome Size Effect On The Rate Of Crossovers In The Meiotic Yeast Saccharomyces Cerevisiae, Lanie Maria Galland

Master's Theses

Meiosis is a specialized type of cell division characterized by a single round of DNA replication and two rounds of chromosome segregation, ultimately resulting in four haploid cells. During meiosis I, chromosomes align and reciprocal recombination results in the formation of a crossover, creating the tension required to properly segregate homologs during the first round of meiosis.

Two mechanisms involved in regulating the occurrence of crossing over are assurance and interference. Crossover assurance describes the phenomenon that at least one crossover will form between each pair of homologous chromosomes during prophase I. Crossover interference, on the other hand, describes the …

Cooperative Construction In Schools In California, John Mauck Donley

Master's Theses

Cooperative Construction in Schools in California

John M. Donley

The construction industry has lost efficiency since 1964, while becoming increasingly more litigious. Schools in California can ill afford the time to allow the construction industry time to fully evolve. It may take years or decades to fully improve the efficiency of, and reduce the conflict within the construction industry.

At the same time, the construction industry has developed new processes to improve efficiency and reduce conflict. These processes are beginning to be broadly embraced by the industry. They all contain cooperative elements. Taken together they represent a new organizing principle …

Photosynthetic Thermal Tolerance And Recovery To Short Duration Temperature Stress In Desert And Montane Plants: A Comparative Study, David William Gallagher

Master's Theses

• Climate change models predict an increase in frequency and amplitude of extreme weather events, including heat waves. To better predict how the composition and distribution of plant assemblages might respond to these changes in temperature, it is important to understand how species currently respond to these extremes. Photosynthetic thermal tolerance (T₂₅)and photosynthetic recovery (RT₂₅) were quantified in 27 species. We also studied the relationships between T₂₅, RT₂₅ and leaf mass per area (LMA). Leaf temperature was also monitored in the field.
• Leaves used in this study were collected from two distinct …

Dual High-Voltage Power Supply For Use On Board A Cubesat, Nicholas Weiser

Master's Theses

Since their conception in 1999, CubeSats have come and gone a long way. The first few that went into space were more of a “proof of concept,” and were more focused on sending simple data and photographs back to Earth. Since then, vast improvements have been made by over 40 universities and private firms, and now CubeSats are beginning to look towards interplanetary travel. These small satellites could provide a cost effective means of exploring the galaxy, using off the shelf components and piggy-backing on other launch vehicles with more expensive payloads. However, CubeSats are traditionally launched into Low Earth …

Consumer Evaluation Of Cheese: Linking Hedonics, Emotions And Perception Of Product Attributes, Kristine Patricia Martinez

Master's Theses

As the food industry continues to grow and the marketplace becomes saturated with similar products, consumer researchers and sensory scientists are looking to dig deeper into the minds of consumers to reveal greater distinctions between products and ultimately deliver multi-dimensionally desirable products to consumers. Concurrently, rates of adult and childhood obesity have been increasing nationwide. Food companies are now facing a paradigm shift as health initiatives and consumers are beginning to demand healthier alternatives to commonly consumed food products. With this in mind, it has become imperative to identify product attributes that drive consumption so they can be replicated in …

Neuromodulation: Action Potential Modeling, Vladimir Ruzov

Master's Theses

There have been many different studies performed in order to examine various properties of neurons. One of the most important properties of neurons is an ability to originate and propagate action potential. The action potential is a source of communication between different neural structures located in different anatomical regions. Many different studies use modeling to describe the action potential and its properties. These models mathematically describe physical properties of neurons and analyze and explain biological and electrochemical processes such as action potential initiation and propagation. Therefore, one of the most important functions of neurons is an ability to provide communication …

Design And Validation Of A Wearable, Continuous, And Non-Invasive Hydration Monitor That Uses Ultrasonic Pulses To Detect Changes In Tissue Hydration Status, Zoie Engman

Master's Theses

Chronic dehydration is an endemic problem for many population groups. Current methods of monitoring hydration status are invasive, time consuming, cannot be performed while exercising, and require lab resources. A proposed solution is a wearable, continuous, and non-invasive device that uses harm-free ultrasonic pulses to detect changes in tissue hydration status over time. Customer and engineering requirements were defined and used to guide the design process. Literature reviews were performed to identify essential information on dehydration, assess current methods, discover state of the art devices, and describe ultrasonic theory. Market research was performed to identify athletes as the target population …

Thermal Numerical Analysis Of Vertical Heat Extraction Systems In Landfills, Michael Thomas Onnen

Master's Theses

An investigation was conducted to determine the response of landfills to the operation of a vertical ground source heat pump (i.e., heat extraction system, HES). Elevated landfill temperatures, reported various researchers, impact the engineering performance of landfill systems. A numerical model was developed to analyze the influence of vertical HES operation on landfills as a function of climate and operational conditions.

A 1-D model of the vertical profile of a landfill was developed to approximate fluid temperatures in the HES. A 2-D model was then analyzed over a 40 year time period using the approximate fluid temperatures to determine the …

A Multi-Gpu Compute Solution For Optimized Genomic Selection Analysis, Trevor Devore

Master's Theses

Many modern-day Bioinformatics algorithms rely heavily on statistical models to analyze their biological data. Some of these statistical models lend themselves nicely to standard high performance computing optimizations such as parallelism, while others do not. One such algorithm is Markov Chain Monte Carlo (MCMC). In this thesis, we present a heterogeneous compute solution for optimizing GenSel, a genetic selection analysis tool. GenSel utilizes a MCMC algorithm to perform Bayesian inference using Gibbs sampling.

Optimizing an MCMC algorithm is a difficult problem because it is inherently sequential, containing a loop carried dependence between each Markov Chain iteration. The optimization presented in …

Laser Speckle Imaging: A Quantitative Tool For Flow Analysis, Taylor A. Hinsdale

Master's Theses

Laser speckle imaging, often referred to as laser speckle contrast analysis (LASCA), has been sought after as a quasi-real-time, full-field, flow visualization method. It has been proven to be a valid and reliable qualitative method, but there has yet to be any definitive consensus on its ability to be used as a quantitative tool. The biggest impediment to the process of quantifying speckle measurements is the introduction of additional non dynamic speckle patterns from the surroundings. The dynamic speckle pattern under investigation is often obscured by noise caused by background static speckle patterns. One proposed solution to this problem is …

Monarch Butterfly (Danaus Plexippus) Tree Preference And Intersite Movement At California Overwintering Sites, Jessica Lynn Griffiths

Master's Theses

Managing Monarch butterfly overwintering groves: making room among the eucalyptus

Proper management and conservation of the coastal California overwintering sites used by western Monarch butterflies (Danaus plexippus L.) is critical for continued use of these sites by monarchs. Many management efforts are currently concentrating on eucalyptus-only sites because of the prevailing notion that monarchs prefer eucalyptus over native tree species. Yet, whether a preference exists or not has never been tested. Herein, we test the “eucalyptus preference” hypothesis with data from five overwintering sites comprised of blue gum eucalyptus (Eucalyptus globulus) and at least one other native tree …

Development Of Novel Anti-Microbial Plant Essential-Oil Treatments To Reduce The Postharvest Incidence Of Botrytis Cinerea Of Strawberries, Ryan C. Brantley

Master's Theses

Fresh strawberries have grown into a multi-billion dollar industry over the last few decades in California. Botrytis cinerea or grey mold, is the worst pathological disorder affecting strawberry fruit causing cull rates of up to 30% in the worst seasons. Strawberry fruits are fragile and have a short shelf-life making timely harvest, cooling, and marketing an absolute necessity. The current industry standard is to ship strawberries in an overwrapped CO₂-enriched headspace pallet in refrigerated trucks at 33 °F. However, B. cinerea has the ability to grow at very low temperatures and continues to plague the strawberry industry during postharvest storage …

A Proof Of Concept For Crowdsourcing Color Perception Experiments, Ryan Nathaniel Mcleod

Master's Theses

Accurately quantifying the human perception of color is an unsolved prob- lem. There are dozens of numerical systems for quantifying colors and how we as humans perceive them, but as a whole, they are far from perfect. The ability to accurately measure color for reproduction and verification is critical to indus- tries that work with textiles, paints, food and beverages, displays, and media compression algorithms. Because the science of color deals with the body, mind, and the subjective study of perception, building models of color requires largely empirical data over pure analytical science. Much of this data is extremely dated, …