Digital Commons Network^™

A Hybrid Deep Learning Model Combining Cnn And Extreme Learning Machine For Cyberattack Classification, Israa S. Kamil

Journal of Intelligent Informatics, Networking, and Cybersecurity

As ransomware attacks and zero-day exploits grow sophisticated, the need for intelligent, accurate systems to detect such threats becomes clearer. In this paper, a hybrid learning model based on Convolutional Neural Networks (CNNs) and Extreme Learning Machine (ELM) is presented to improve multiclass classification performance for cybersecurity applications. The framework combines CNNs' hierarchical feature learning with ELMs' fast classification. An attention mechanism that assigns weights to each feature based on importance is included in the final model. The hybrid model performed well on the metrics: precision = 0.97, recall = 0.98, and F1- score = 0.97, and, as expected from …

Beyond Histone Demethylation: Mechanisms Of N‑Alkyl Consecutive Oxidations By The Non-Heme Fe(Ii)/2-Oxoglutarate Oxygenase Kdm6b, Simahudeen Bathir Jaber Sathik Rifayee, Sudheesh Devadas, Midhun George Thomas, Bhargav Varada, Ethan Sommer, Cassandra Talaba, Christopher Schofield, Christo Christov

Michigan Tech Publications

The nonheme Fe-(II)/2-oxoglutarate (2OG)-dependent histone demethylase KDM6B (JMJD3) has demonstrated a capacity for diversity in the oxidative transformations of N-alkylated lysine residues in histone H3 peptides; however, the mechanisms of such dealkylations, compared with standard KDM-catalyzed demethylations, remain unexplored. We implemented molecular dynamics and quantum mechanics/molecular mechanics to investigate the catalytic strategies for the sequential oxidation reactions of KDM6B with different N-alkylated forms of lysine K27 in the H3 peptide chain, that is N , N -methyl ethyl lysine () and N -isopropyl lysine (). The results for sequential oxidations, which yield alcohol, aldehyde, and then carboxylic acid products, reveal …

Stop Blaming My Users: Illumination Of The Technocentric Mythos Bias, Ervin H. Frenzel, Richard Lightcap

Journal of Cybersecurity Education, Research and Practice

 Abstract -This conceptual essay addresses the need for systemic and systematic transdisciplinary analytical techniques within cybersecurity and technical security. This conceptual essay is contingent upon recognition that cybersecurity is not simply technical in nature, it does not need an adversary, and more importantly it is based upon systems engineering and systems thinking.  The essay contributes a socio-technical attribution chain and field-specific ontology/taxonomy which distinguish user-triggered events from root causes, latent conditions, technical debt, validation failures, governance failures, and attribution bias before assigning responsibility to end users. It systematically defines an ontology inclusive of developer technical debt, organizational debt arising from …

Toxic Relationships: Characterization Of A Putative Virally Encoded Toxin In The Thermophilic Archaeal Fusellovirus Ssv1, Jonathan C. Abshier, Patrizia L. Alpapara, Guasåli Tomokane, Kenneth M. Stedman

Center for Life in Extreme Environments Publications

Mechanisms for the maintenance of chronic viruses are poorly understood, particularly for archaeal viruses. Here, we identify the product of Sulfolobus spindle-shaped virus 1 (SSV1) ORF a291 as a putative virally encoded toxin required for growth inhibition but dispensable for viral replication and virion production. Viruses lacking ORF a291 replicated their genomes and formed morphologically normal spindle-shaped particles, yet failed to inhibit the growth of uninfected Saccharolobus solfataricus. Substitution of residues at a predicted N-terminal signal peptide cleavage site abolished growth suppression without affecting replication, suggesting that secretion is essential for toxin function. Despite primary sequence divergence among fusellovirus …

Micro-Computed Tomography Analysis Of Fibroblast Growth Factor-2 Activity-Induced Healing Of Dentocraniofacial Bones In Rodent Models: A Systematic Review, Fadhlil Ua. Rahman, Azhari Azhari, Lusi Epsilawati, Ria N. Firman, Barunawaty Yunus, Irfan Sugianto, Muliaty Yunus

Journal of Dentomaxillofacial Science

Objective: This study aimed to assess the impact of fibroblast growth factor-2 (FGF-2) on dentocraniofacial bone healing in rodent models using micro-computed tomography (micro-CT).

Materials and Methods: A systematic review was conducted following PRISMA 2020 guidelines. A literature search was conducted using the PubMed, Embase, Web of Science, ScienceDirect, and Wiley Online Library databases. This review included in vivo rodent studies that examined increased FGF-2 activity in craniofacial bones using micro-CT. Methodological quality and risk of bias were assessed using the ARRIVE 2.0 guidelines and the Systematic Review Center for Laboratory Animal Experimentation tool.

Results: A total of 5 studies …

Looking Inwards, Turning Away: A Review Of Rehan Abeyratne’S ‘Courts And Lgbtq+ Rights In An Age Of Judicial Retrenchment’, Jwalika Balaji

Socio-Legal Review

This review engages with Rehan Abeyratne's book Courts and LGBTQ+ Rights in an Age of Judicial Retrenchment, which examines judicial responses to LGBTQ+ claims over the past two decades across three jurisdictions — the United States, India, and Hong Kong. Abeyratne argues that the apex courts in these countries have grown more inward-looking and have enabled the rise of illiberal and authoritarian forces; however, LGBTQ+ rights have emerged as an exceptional site where these courts continue to extend minimal remedies and adopt a tone of empathy, positioning themselves as the last bastion of liberal constitutionalism. Focusing on recent developments …

Academics’ Views On Integrating Climate Change In Nursing And Midwifery: A Mixed Methods Study, Seda Guney, Seda Sarikose, Tuba Sengul, Nurten Kaya

International Nursing Research Congress (INRC)

Background: Climate change is a major global health challenge requiring integration into nursing and midwifery education [1]. Yet, evidence on educators’ awareness, concern, and curricular engagement remains limited [2].

Purpose: This study explored academics’ awareness and worry about climate change and their perspectives on integrating related content into nursing and midwifery curricula.

Design and Methods: A convergent parallel mixed-methods design guided by the Sustainability in Global Nursing framework [3] was used. Quantitative and qualitative data were collected concurrently (March–August 2025). The quantitative phase involved 160 faculty members from nursing and/or midwifery programs completing an online survey that included the Climate …

Bureaucratic Politics Of Reforestation In Indonesia's Conservation Areas: Turf Protection, Competing Mandates, And Implementation Deadlocks, Arief Setiyo Utomo, Pande Made Kutanegara, Ahmad Maryudi

Forest and Society

In response to extensive deforestation and land degradation, the Indonesian government has made reforestation one of its top policy priorities. Although several reforestation programs have been implemented, their outcomes have often been constrained by governance-related challenges. This study examines the political dynamics of reforestation in conservation areas by analyzing interactions between two key bureaucracies within the Ministry of Forestry: the Directorate General of Watershed Management and Forest Rehabilitation (DG-WMFR) and the Directorate General of Natural Resources and Ecosystem Conservation (DG-NREC). Drawing on the bureaucratic politics perspective, we show how competing mandates, organizational interests, and bureaucratic turf protection motives shape the …

Impacts Of Physical Extremes On The Reproductive Output And Dispersal Of Stephanocystis Osmundacea, Jessica J. Franks

Master's Theses

Population replenishment is vital for species survival and depends on reproductive output and recruitment success. Many marine organisms rely on both short- and long-distance dispersal to sustain and expand their populations. Marine dispersal involves microscopic or planktonic stages that are influenced by currents, physical processes, and biological traits such as planktonic duration and sinking behavior. Depth and light gradients affect primary producer growth, impacting reproduction, especially in morphologically plastic seaweeds. This study examined how depth-related factors influence reproductive morphology, reproductive output, and dispersal of the subtidal brown seaweed Stephanocystis osmundacea in central California. Using field, lab, and computational methods, depth-driven …

Investigating Learning In Immersive Virtual Reality, Andrew Negrette

Master's Theses

Students often struggle with learning challenging subjects throughout their education. This also tends to lead to lower enjoyment and science self-efficacy for learning. Some subjects may be better represented in 3-dimensional spaces utilizing immersive virtual reality (IVR). Recently, IVR headsets have become more accessible and affordable and therefore are in wider use. The purpose of this study was to investigate whether differences in science selfefficacy, enjoyment, presence and knowledge retention exist between IVR and controls on an ocean acidification lesson. I predicted that participants taking the lesson in IVR would score higher compared to a laptop on all measures, due …

Genetic Associations With Pulmonary Embolism Among Those With A Deep Vein Thrombosis: The International Network Against Venous Thrombosis Consortium, Susana Lozano-Esparza, Gabrielle E Shakt, Jennifer A Brody, Angel Martinez-Perez, Matthew P Conomos, Marine Germain, Katharine R Clapham, Maris Teder-Laving, Astrid Van Hylckama Vlieg, Anni Kauko, Florian Thibord, Ohanna C L Bezerra, Girish N Nadkarni, Gaëlle Munsch, Therese Haugdahl Nøst, Ellen L Goode, Yuekai Ji, Daniel I Chasman, Alexander P Reiner, Constance Turman, Kerri L Wiggins, Colleen M Sitlani, Juan Carlos Souto, Pamela L Lutsey, Tiffany Bellomo, Estonian Biobank, Million Veteran Program, Ruifang Li-Gao, Aleksi Kristian Winstén, Ming-Huei Chen, Ron Do, Lénaick Gourhant, Kristian Hveem, Sebastian M Armasu, Noémie Saut, Nathan Pankratz, Franco Giulianini, Jeffrey Haessler, Mingyang Song, Robert Olaso, Eric Boerwinkle, Daniel Dochtermann, Jose Manuel Soria, Stephen S Rich, David Smadja, Satoshi Koyama, Frits R Rosendaal, Teemu Niiranen, France Gagnon, Ha My T Vy, Neil Zakai, Catherine Lemarie, Anne Heidi Skogholt, Jean-François Deleuze, James S Pankow, Paul M Ridker, Yuxi Liu, Kenneth M Rice, Saiju Pyarajan, Mary Cushman, Jospeh Emmerich, Bruce M Psaty, Pradeep Natarajan, Andrew D Johnson, Marc A Rodger, Pierre Suchon, Francis Couturaud, Pierre-Emmanuel Morange, Weihong Tang, Charles Kooperberg, Christopher Kabrhel, Maria Sabater-Lleal, David-Alexandre Trégouët, Alisa S Wolberg, Scott M Damrauer, Nicholas L Smith

Children’s Nutrition Research Center Staff Publications

Background: Venous thromboembolism (VTE) includes deep vein thrombosis (DVT) and pulmonary embolism (PE), the latter of which often originates from DVT and can be fatal.

Objectives: The aim of this study was to identify causal genetic factors that alter risk of PE among those with a presumed DVT.

Methods: Using a case-only design of VTE, we conducted meta-analyses of genome-wide association studies (GWASs) in the International Network Against Venous Thrombosis Consortium. Among participants diagnosed with VTE, each study identified those who had a clinically reported PE, with or without a clinically reported DVT; those remaining had isolated DVT. Logistic regression …

Effects Of Variable Light On Sexual Competition In Kelp Species, Jaycee Lanza

Master's Theses

As foundation species, kelps significantly influence the structure and composition of their surrounding habitat by modifying environmental conditions, including light levels, currents, nutrient availability, sedimentation, and seawater chemistry. Light, in particular, is a critical resource that varies greatly within kelp forests, affecting kelp reproductive processes such as gametogenesis, fertilization, and recruitment, which are vital to population persistence. Biological factors, such as density-dependent competition for resources and sexual competition with other kelps, can also affect such processes. To understand how environmental variables influence kelp populations, it is crucial to recognize their combined effects. This study investigated the impacts of light levels …

The Characterization Of Bridging Waters And Ions In Protein-Rna Complexes, Leslie Duong

Master's Theses

Here we explore the role of bridging waters and ions in protein-RNA complexes. From an initial set of 33 high-resolution X-ray crystal structures, 17 were identified such that protein-RNA complex and component protein and RNA chains were found within 1 pH unit as indicated by crystallization conditions and their diffraction temperatures meeting one of two ranges: above or below freezing. To define an even more realistic set, 12 unbound-unbound and unbound-pseudo-unbound complexes from the 17-set were studied, showing the large fraction of complexes indicating more protein and RNA water hydrogen bonding in their actual interfacial regions as opposed to those …

Hydrus Modeling Of A Sahelian Agroforestry System: An Investigation Of Local Water Balance, Nicole A. Butler

Master's Theses

Effects of drought can devastate agricultural productivity in the West African Sahel. Climate complexities, such as lower-than-average rainfall and high winds, can lead to crop failure and low yields, thereby creating food security issues. Agriculture in the Sahel is primarily rainfed, and the rain is becoming increasingly unpredictable due to the changing climate. The method of intercropping groundnut (Arachis hypogea) and pearl millet (Pennisetum glaucum) with the shrub species Piliostigma reticulatum can combat the effects of climate change and restore degraded soil. Intercropping can increase water availability and crop yield when compared to crops grown in the absence of shrubs. …

Multiple Perspectives On Inclusion In Science For Students Receiving Support From The Learning Center (Tlc) Program, Jeremy C. Adams

Dissertations

Almaden Country Day School (ACDS) is a small, independent private school located in the Almaden Valley neighborhood of south San Jose, California. Currently serving over 400 students, the school has been in operation for over 43 years. Nan Hunter, its founder, set out to build a school community that could “discover the gifts in every child.” As part of this mission, the school sought to support neurodiverse students with accommodations as part of its general education program. In September of 2017, the school’s leaders committed more deeply to this aspect of the school’s mission when they opened The Learning Center …

Out Of The Briar Patch: Storying Resistance Among Black Male Survivors Of Childhood Sexual Abuse And Its Impact On Their Educational Experiences, Anthony Animuntu

Dissertations

This study examines the lack of support within educational settings for Black boys who are survivors of childhood sexual abuse (CSA). Despite growing attention to trauma-informed practices, the experiences of Black male survivors remain largely absent from educational research. Using a qualitative, phenomenological approach informed by critical autoethnography, semi-structured interviews were conducted with five African American men reflecting on their schooling experiences as CSA survivors. Axial analysis revealed four central themes: silence as survival and strategic invisibility, shame and internalized distress, institutional misrecognition, and the transformative role of relational care and educational kinship. Findings indicate that participants’ trauma was often …

Can Curriculum Improve Confidence? Examining The Impact Of The Implementation Of The California Math Framework On Math Anxiety Levels Of Preservice Teachers In California, Amanda Lubbs

Dissertations

In 2010, the state of California adopted the Common Core State Standards (CCSS) as an effort to ensure students were graduating high school as college and career ready. Shortly after, in 2013, the California Board of Education adopted the California Math Framework (CMF) as a tool for schools to implement the CCSS for Mathematics (CCSSM). Its recommendations incorporated research-based techniques to alleviate math anxiety through the use of explorative learning experiences that help students understand math skills in applied contexts, and by de-emphasizing rote practice and memorization. This study inquires about the framework’s effectiveness in mitigating math anxiety among pre-service …

Supports And Challenges For Esns In Higher Education: A State University Qualitative Study, Dilkash Ahmed

Dissertations

Although extensive support needs students (ESNS) benefit from access to inclusive higher education opportunities, research shows that navigating college settings has been challenging for these students. This study examined the supports available to ESNS enrolled in college classes and the challenges they encounter within a university setting. Using a State University as the research site, this qualitative study collected data through semi-structured interviews, observations, and reflection responses to capture the experiences of ESNS, faculty, and peer mentors in higher education. The study examined ESNS experiences in classes through the perspectives of faculty on teaching ESNS, peer mentors’ (who support students …

Ensuring Effective, Efficient, And Equitable Libraries In A Post-Pandemic Community College Environment, Jeffery M. Sundquist

Dissertations

Using a case study model, this dissertation investigated student needs regarding library support in a post-pandemic community college environment, using data from student focus group interviews. These needs were juxtaposed with perceived student needs using library employee survey data. Based on the interpretation of data grounded in a critical librarianship framework, the author created action items for college library leaders. To provide effective, efficient, and equitable onsite libraries and library services, the author recommends (a) designing for psychological safety and belonging, (b) embedding visible human support, and (c) conducting equity-oriented space audits. To provide effective, efficient, and equitable online libraries …

Childbirth Experiences Of The Bangladeshi Immigrant Women In South Florida, Tahsina Ferdous

Electronic Theses and Dissertations

This qualitative research was conducted among Bangladeshi immigrant women living in South Florida who have given birth here. Twenty Bangladeshi immigrant women were selected using the snowball sampling method from various cities in South Florida. The unit of analysis was the individual Bangladeshi immigrant woman, focusing on her experiences and behavior during pregnancy and childbirth. Semi-structured interviewing and case study methods were used for data collection. The results reveal the coping strategies and the navigation process of socio-cultural transition from non-mother to mother, and the Bangladeshi traditional views regarding beliefs, rituals, and practices of Bangladeshi immigrant women regarding their pregnancy …

Novel Integrative Mobilizable Elements (Imes) In Vibrio Chromosomes Linked To Translucent Post-Larvae Disease (Tpd), Ruyi Hao, Yan Fang, Yang Yang, Mingshu Yang

School of Biological and Marine Sciences

Translucent post-larvae disease (TPD), caused by specific Vibrio parahaemolyticus strains, poses a global threat to shrimp aquaculture. A key virulence factor in these strains is the vhv gene cluster, which encodes toxins central to TPD pathogenesis. While plasmid-borne vhv genes have been extensively studied, the chromosome-borne vhv genes in TPD-associated strains remain poorly characterized. In this study, we systematically analyzed integrative mobilizable elements (IMEs) on the small secondary chromosomes of TPD-associated V. parahaemolyticus. Seven nearly identical novel IMEs harboring full-length vhv clusters were identified from seven independent strains. These IMEs encode a tyrosine recombinase for site-specific chromosomal integration, yet lack …

Beyond Histone Demethylation: Mechanisms Of N-Alkyl Consecutive Oxidations By The Non-Heme Fe(Ii)/2-Oxoglutarate Oxygenase Kdm6b, Simahudeen Bathir Jaber Sathik Rifayee, Sudheesh Devadas, Midhun George Thomas, Bhargav Varada, Ethan Sommer, Cassandra Talaba, Christopher Schofield, Christo Christov

Michigan Tech Publications

The nonheme Fe(II)/2-oxoglutarate (2OG)-dependent histone demethylase KDM6B (JMJD3) has demonstrated a capacity for diversity in the oxidative transformations of N^ε-alkylated lysine residues in histone H3 peptides; however, the mechanisms of such dealkylations, compared with standard KDM-catalyzed demethylations, remain unexplored. We implemented molecular dynamics and quantum mechanics/molecular mechanics to investigate the catalytic strategies for the sequential oxidation reactions of KDM6B with different N-alkylated forms of lysine K27 in the H3 peptide chain, that is N^ε, N^ε-methyl ethyl lysine (Lys(Me/Eth)) and N^ε-isopropyl lysine (Lys(iPr)). The results for sequential oxidations, which yield alcohol, aldehyde, and …

Physiological Impacts Of Plastic Ingestion On Sea Turtles, Heather Seaman

Electronic Theses and Dissertations

Over the years, plastic production has increased to astonishing numbers and is predicted to continue. Over time, environmental plastic will degrade into smaller pieces, which sea turtles can mistake for prey. Chemicals associated with plastics have been identified in tissues of sea turtles, and some are suspected to be endocrine disruptors. Sea turtles are known to ingest plastics, but more information is needed to illuminate the potential physiological impacts plastic chemicals may have on sea turtles. This study compared plastic ingestion and toxicant levels across species and ontogenically, as different species and life stages inhabit different areas which will influence …

Hybrid Microbial-Enzymatic Electrosynthesis For De Novo Decanoic Acid Production From Co2, Yongze Wang, Wenjin Dong, Meijing Wei, Su Song, Zhiqing Huang, Yaojie Liu, Shelley D. Minteer, Hui Chen

Chemistry Faculty Research & Creative Works

The steady increase in atmospheric CO₂ levels is a key driver of anthropogenic climate change, prompting widespread global concern. In this context, the sustainable conversion of CO₂ into value-added chemicals represents an attractive route toward carbon-neutral manufacturing and green chemistry. Here, we developed a proof-of-concept hybrid microbial-enzymatic electrosynthesis system that combines microbial electrosynthesis (MES) and enzymatic electrosynthesis (EES) to convert CO₂ into decanoic acid under ambient conditions, using electrical energy. In the MES module, CO₂ was converted to acetate via the Wood–Ljungdahl pathway of Clostridium ljungdahlii, using H2 produced by electrochemical water splitting as the electron …

In Silico Analysis Of Vaginal Microbiota In Female Reproductive Health Using Ncbi Database And Blast, Arleni Bustami, Ariyani Kiranasari, Engla Merizka

Indonesian Journal of Medical Chemistry and Bioinformatics

Background: The vaginal microbiota plays a critical role in maintaining female reproductive health. Beneficial bacteria, particularly Lactobacillus species, contribute to vaginal homeostasis, whereas dysbiosis-associated bacteria such as Gardnerella vaginalis and Prevotella bivia have been linked to bacterial vaginosis and reproductive tract disorders. Publicly available nucleotide databases and bioinformatic tools provide accessible resources for studying microbial species associated with vaginal health. Objective: This study aimed to perform a simple in silico analysis of representative vaginal microbiota using publicly available 16S rRNA gene sequences retrieved from the NCBI database and analyzed using BLAST and phylogenetic approaches. Methods: Representative 16S rRNA gene sequences …

The Biological Response Of Human Np Cells To Soluble Collagen Vi, Jonathan Dalton, Srikanth N. Divi, Yasmine Eichbaum, Panya Luksanapruksa, Mitchell K. Ng, Rachel Huang, Alan S. Hilibrand, Alex R. Vaccaro, Dessislava Z. Markova, Christopher K. Kepler

Department of Orthopaedic Surgery Faculty Papers

Extracellular matrix (ECM) dysregulation plays a significant role in the development of intervertebral disc disease (IVDD). The ECM around the chondrocytes in the nucleus pulposus (NP) is unique in that it contains ColVI. While linked to chondrocyte proliferation in cartilage, ColVI's specific role in IVD degeneration is less clear. Our aim was to determine the expression of ColVI in NP cells from degenerative IVDs, along with the effects of soluble ColVI on NP cells. Thus, we compared the expression levels of ColVI in human degenerated IVD samples, along with the effect of soluble ColVI on primary human NP cells. A …

Using Fecal Diagnostic Tests To Assess The Prevalence Of Fasciola Hepatica In The United States Sheep Population, Brooke A. Delcambre

LSU Master's Theses

Fasciola hepatica, the common liver fluke, is endemic in the Pacific Northwest and Gulf Coast of the United States. To provide updated information on F. hepatica prevalence, as part of the USDA National Animal Health Monitoring System Sheep 2024 study, sheep operations in the top 30 sheep-producing states were identified, and producers were invited to participate. For each operation, feces were collected from at least eight sheep and pooled to form a single composite sample. Two methods were used to identify sheep infected with F. hepatica: fecal sedimentation and indirect sandwich ELISA. At the national level, across 318 operations, 7.1 …

Predictors Of Possible Sarcopenia And The Role Of Psychomotor Frailty In People With Schizophrenia, Pei-Yi Chen, Yia-Ping Liu, Chiu-Yueh Yang

International Nursing Research Congress (INRC)

Background: People with schizophrenia commonly experience physical health decline, including reduced muscle mass and strength due to medication and a sedentary lifestyle, leading to sarcopenia and greater health risks. In 2019, the Asian Working Group for Sarcopenia (AWGS) introduced "possible sarcopenia" to promote early detection and prevention.

Objective: This study aims to investigate the prevalence and predictors of possible sarcopenia in patients with schizophrenia spectrum disorder.

Methods: This multicenter cross-sectional study recruited 216 participants aged 18–65 from two psychiatric teaching hospitals in northern Taiwan through convenience sampling. Structured questionnaires assessed demographics, the Frailty Assessment Scale for Schizophrenia (FASS), Positive and …

Analysis Of Inorganic Element Content And Health Risk Assessment Of Ranunculi Ternati Radix From Different Origins, Li Juan, Geng Xiaotong, Yao Weijie, Zhou Tao, Bi Jingjing

Food and Machinery

[Objective] The variations in inorganic element content and the safety of heavy metals and harmful elements in Ranunculi ternati radix from different origins were investigated. [Methods] The quantitative analysis of 24 elements in the samples was performed by inductively coupled plasma mass spectrometry (ICP-MS). Health risk assessment was conducted using estimated daily intake (EDI), target hazard quotient (THQ), and carcinogenic risk (CR). Principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) were used for origin identification analysis and differential element screening. [Results] All 25 Ranunculi ternati radix samples from different origins contain abundant macronutrients (K, Na, Ca, and Mg) …

Rapid Detection Of Viable Salmonella Based On Specific Phage Infection Combined With Fluorescent Rpa, Liu Daofeng, Zhai Yujie, Tan Yucheng, Shan Shan, Lu Jun, Zhong Ziqing

Food and Machinery

[Objective] To establish a rapid detection method for viable Salmonella based on specific phage infection and recombinase polymerase amplification (RPA) in response to the key challenge that traditional molecular biological detection methods have difficulty in conveniently distinguishing between viable and non-viable foodborne pathogens. [Methods] Taking advantage of the characteristic that O-1 phage only proliferates in viable bacteria, the phage is added to the enriched sample. After further incubation, total DNA is extracted. Fluorescent RPA is used to determine whether the phage has significantly proliferated, so as to judge the presence of viable Salmonella in the original sample. [Results] After optimization, …