Digital Commons Network^™

Examining The Impacts Of State Route 101 On Wildlife Using Road Kill Surveys And Remote Cameras, Sara Ann Snyder

Master's Theses

Roads can negatively impact the survival of wildlife populations through additional mortality from road kill and population fragmentation caused by road avoidance behaviors. The 11.9 mile section of State Route 101 between the towns of San Luis Obispo and Atascadero, CA, USA, cross a mountain lion movement corridor and an area important to maintaining ecological connectivity between protected lands in the Los Padres National Forest to the north and south.

I examined the spatial patterns and landscape and roadway factors associated with road kill occurrence for six taxa; large mammals, mesocarnivores, squirrels, rabbits, birds and raptors. Between 1 May 2009 …

Chromatographic Analysis Of Fatty Acids Using 9-Chloromethyl-Anthracene And 2-Bromomethyl-Anthraquinone, Jesus B. Tapia

Master's Theses

Fatty acids are carboxylic acids with long alkane or alkene chains and are important components of living organisms. The presence of the carboxylic acid is analytically very important since it allows for derivatization with fluorescence reagents such as 9-chloromethyl-anthracene (9-CMA) for HPLC analysis. A method for the analysis of fatty acids using 9-CMA and 2-bromomethyl-anthraquinone (MAQ-Br) was developed. The method utilizes a modified protocol previously used for the analysis of short-chain carboxylic acids with 9-CMA. The modified protocol was applied to medium- and long-chain fatty acids for analysis by HPLC with UV-visible and fluorescence detection. After successful derivatization and analysis …

Predictors Of Major Commitment, Anna Jill Womack

Master's Theses

Contextual (e.g. job fit, job involvement), individual (e.g. job satisfaction, need for achievement), and demographic (e.g. gender, educational level) factors have been related to forms of career commitment (i.e. affective, continuance, and normative commitment), highlighting that the commitment one feels toward his or her career is a complex variable. Furthermore, commitment has been associated with intent to remain within a profession or organization (Bowling, Beehr, & Lepisto, 2006; Den Hartog & Belschak, 2007; Duffy, Dik, & Steger, 2011; Goulet & Singh, 2002), suggesting that commitment is an important component of retention within a career. Correspondingly, commitment to one's academic major …

Self-Assembled Gold Nanoplexes For Cancer-Targeted Sirna Delivery, Yongliang Shi

Master's Theses

Through layer-by-layer method, the authors have constructed three Au nanoplexes: AuPEI/RNA/PEI, AuPEI/RNA/PEI-mPEG, and AuPEI/NA/PEI-PEG-FA. All the nanoplexes are characterized by UV-vis spectrometry, DLS, and zeta potential. The surface density of the first layer PEI and second layer RNA were also determined. Moreover, the Au nanoplexes can protect siRNA from RNase degradation and are stable in cell culture medium.

siGLuc-ppp, ssRNA₈₀, and siF17 were delivered by the assembled Au nanoplexes, and the results were analyzed by GLuc assay, TB assay, and Luciferase assay. In terms of RNA delivery, the Au nanoplexes AuPEI/RNA/PEI exhibited excellent gene silencing efficiency (or causing …

Methane Dynamics In St. Louis Bay, Mississippi, Hannah Marie Roberts

Master's Theses

A method of dissolved methane analysis was developed utilizing cavity ring-down spectroscopy and headspace equilibration. Samples of 70 mL were collected in 140 mL plastic syringes and equilibrated with a methane free headspace. Reproducibility was high (i.e. 4% typical RSD), and samples were successfully measured in the low nanomolar to high micromolar range. During method development, multiple research cruises were undertaken in the northern Gulf of Mexico. Stations included the Orca Basin, the Deepwater Horizon site, and the surrounding area. The Deepwater Horizon site showed no continuing leakage from October 2010 to June 2013. Samples collected from the northern Gulf …

A Description Of Vocalizations And Their Association With Mouthing Behaviors And Social Context In Bottlenose Dolphins, Tursiops Truncatus, Christina Elyse Perazio

Master's Theses

Dolphin communication is multimodal and incorporates physical behaviors and vocalizations. Dolphins often exchange information with conspecifics using different types of vocalizations, and these vocalizations are sometimes associated with specific behaviors. However, the relationship of vocalization type and mouthing behavior type has not been investigated. This thesis examines simultaneous acoustic and visual recordings of bottlenose dolphins (Tursiops truncatus) to determine the relationship between type of mouthing behavior and type of vocalization (whistle, whistle-squawk, chirp, moan, burst-pulse type A, burst-pulse type B, and click trains). The role of the social context of a mouthing behavior is also evaluated. Data were obtained opportunistically …

Psychopathology And Sexual Addiction As Correlates Of Disordered Attachment, Natasha Lisa Laurent

Master's Theses

The current study examined associations between insecure adult romantic attachment and symptoms of psychopathology, disordered personality, and problematic sexual behaviors in a clinical sample of 402 men in treatment for sexual addiction. Anxious and avoidant attachment were hypothesized to correlate with certain constructs of psychopathology, disordered personality, and dimensions of problematic sexual behaviors. Multiple regression analyses were conducted to examine the hypothesized relationships using data from scales of the Minnesota Multiphasic Personality Inventory- 2-Revised Form (MMPI-2-RF), the Sexual Dependence Inventory – 4.0 (SDI – 4.0), and the Experiences in Close Relationships-Revised (ECR-R). Results suggested greater similarities than expected across higher-order …

The Ontogeny Of Whistle Production In Infant Atlantic Bottlenose Dolphins (Tursiops Truncatus) During The First Thirty Days Of Life, Brittany Leigh Jones

Master's Theses

The manner in which dolphin calves acquire their whistle repertoire is largely unknown. This paper focuses on whistle development in four bottlenose dolphin calves during the first thirty days of life in order to increase our understanding of the early emergence of whistles and whistle-like vocalizations. The acoustic parameters of whistle-type vocalizations (i.e., whistles and whistlesquawks) that coincided with a bubblestream emission from the focal calf and/or its mother were analyzed, as were the behavioral states of the mother-calf pair during the emission of such vocals. Mother and calf whistle rates are inversely related, with the mother whistling more often …

Assemblage Comparisons Of Living Benthic Foraminifera At Bathyal Sites Oiled And Un-Oiled By The Deepwater Horizon Blowout In The Northeastern Gulf Of Mexico, Valerie Joanna Cruz

Master's Theses

Live benthic foraminiferal assemblages were studied at contaminated and uncontaminated bathyal sites around the wellhead of the Deepwater Horizon oil spill. Samples collected in October of 2010 and 2011 and summer of 2012 were divided into uncontaminated (GIP 12, 21, K, and 25 and Obs0), moderately oiled (GIP 16 and 17) and heavily oiled groups (GIP 15), in which the TPAH concentrations ranged from 29 to 7,553 ng/g in 2010. Metric multidimensional scaling (MDS) and cluster analyses were conducted to compare all surface samples. Additionally, the heavily oiled site (GIP 15) and an uncontaminated site (GIP 25) were studied downcore …

Role Of Msaa Gene In Regulation Of The Msaabcr Operon And Biofilm Development In Staphylococcus Aureus, Ahmed Alzuway

Master's Theses

Staphylococcus aureus is an important human pathogen that causes wide variety of diseases ranging from chronic biofilm associated infection to acute life threatening infection such as bacteremia, pneumonia, osteomyelitis, or endocarditis, despite the progress with antibiotics used in the treatment of bacterial infections. Furthermore, increased use of prosthetic and indwelling devices in modern medical practices has led to increased infections due to S. aureus. Treating S. aureus infections have become difficult owing to its ability to resist most of the antibiotics; this problem is further exacerbated by ability of MRSA strains to form biofilms. Emergence of community-acquired methicillin resistance …

Characterization Of Rain And Stormwater Nitrogen Inputs To The Mississippi Sound: A Landscape Approach, Joshua Michael Allen

Master's Theses

Urbanization in coastal watersheds is becoming an increasingly important issue in the world. Increased impervious surface cover, a factor of urbanization, has drawn more attention to stormwater runoff as a source of contamination to receiving waters. In this study, nitrogen species from rain and stormwater were analyzed across three different landscape types along the Mississippi Sound (hardened, residential, and pristine), as well as from drainage pipes that flow directly into to the Mississippi Sound. Nitrogen stable isotopes were used to trace the stormwater nitrogen to the surface waters and biota within the Sound.

The objective of this study was to …

Nerve Fiber Diameter Measurements Using Hematoxylin And Eosin Staining And Brightfield Microscopy To Assess The Novel Method Of Characterizing Peripheral Nerve Fiber Distributions By Group Delay, Jorge Arturo Vazquez

Master's Theses

Peripheral neuropathies are a set of common diseases that affect the peripheral nervous system, causing damage to vital connections between various parts of the body and the brain and spinal cord. Different clinical conditions are known to selectively impact various size nerve fibers, which often makes it difficult to diagnose which peripheral neuropathy a patient might have. The nerve conduction velocity diagnostic test provides clinically useful information in the diagnosis of some peripheral neuropathies. This method is advantageous because it tends to be minimally invasive yet it provides valuable diagnostic information. However, this test does not determine characteristics of peripheral …

Treatment Of Nitrogen Oxides By Chlorella Vulgaris Algae In Photobioreactors, Steven Shihady

Master's Theses

The effectiveness of algae to treat NO₂and NO in simulated flue gas was tested using Chlorella vulgaris in photobioreactors (PBRs) using NO_xconcentrations between 30 ppm to 780 ppm. NO_xdissolved and reacted in water to form NO₃^-and NO₂^-in the PBR growth medium, providing a nitrogen source that the algae readily assimilated for cell synthesis. Three 20-L photobioreactors were inoculated with a pure culture of C. vulgaris prepared in Bristol growth medium and algae were grown in the PBRs at 25°C and pH of 7.0 in a modified Bristol medium …

Behavioral And Hormonal Flexibility Across Light Environments In Guppies (Poecilia Reticulata), Julia C. Walz

Master's Theses

Behavior may be dramatically influenced by changing environments, and differences in light intensity environments may have important behavioral consequences. One approach to understanding changes in behavior is by studying behavioral syndromes, suites of correlated behaviors reflecting between individual consistencies in behavior expressed within a behavioral situation (e.g., correlations between antipredator behaviors in different habitats), or across behavioral contexts (e.g., correlations among feeding, antipredator, or mating behavior) (Sih et al. 2004a). Guppies (Poecilia reticulata) provide a great model system to study behavior. Guppies are small, freshwater tropical fish that inhabit still pools in swift-flowing streams, and the backwaters of small rivers …

Delineating Wetlands In Serpentinitic Soils, Ariel Nikole Namm

Master's Theses

Wetlands serve an important ecological and functional role in the environment. In order to protect wetlands from degradation, the determination of wetland hydrology, hydrophytic vegetation, and hydric soils is necessary. Soils formed from serpentine parent material on hillside slump blocks do not meet any of the current morphological Field Indicators of Hydric Soils. Although these areas seem to be wetlands based on wetland hydrology and hydrophytic vegetation, there is no approved hydric soil indicator that includes the properties exhibited by serpentinitic soils on these landscapes. The objectives of this research were: (1) to examine soil chemical, physical, and morphological properties …

Assessment Of Microbial Biodegradation Of Mixed Soil Contaminants At The Santa Susan Field Laboratory Using Trflp, Qpcr, And Culturing, Kenny William Croyle

Master's Theses

The potential for biodegradation of contaminants in soil was assessed using an array of molecular methods, including terminal restriction fragment length polymorphism (TRFLP), quantitative polymerase chain reaction (qPCR), and traditional culturing techniques combined with sequencing of the 16S or ITS regions of the cultured bacteria and fungi. Soil was collected from the Santa Susana Field Laboratory (SSFL), which was the site of numerous liquid-propulsion rocket engine tests as well as nuclear energy research and development, which led to contamination of the soil with a wide variety of constituents. The contaminants of interest (COIs) at this site include polychlorinated biphenyls (PCBs), …

A Multicultural Nutrition And Culinary Intervention For Middle School Students: Pink And Dude Chefs, Phase 2, Jaime Lynn Lockhart

Master's Theses

The prevalence of adolescent obesity in the United States has more than quadrupled in the past 30 years, growing from 5% to nearly 21% in 12-19 year olds. Although obesity has many interrelated causes, a lack of knowledge and practical skill to prepare healthy food from scratch is an important factor. Research indicates that nutrition and culinary interventions based on behavioral change theories may improve mediators of healthful eating in adolescents.

Pink and Dude Chefs, Phase 2, an eight-week after-school nutrition and culinary intervention targeted towards middle school students in a predominantly low-income, Hispanic community, was developed and evaluated. The …

A Comparison Of Fatigue During Cardiocerebral Resuscitation With Different Compression Rates Among Layperson And Professional Rescuers, Christopher Cassidy

Master's Theses

Quality chest compressions during Cardiopulmonary Resuscitation (CPR) are vital to maintaining adequate perfusion of oxygenated blood to the organs of the body to sustain life. Over the years, the compression rate recommended in Basic Life Support (BLS)/ Advanced Cardiac Life Support (ACLS) protocols for the best possible outcome has risen, and with that increase there are questions regarding rescuer fatigue and the effectiveness of compressions. Layperson and professional rescuers, answering to an emergency, both maintain continuous chest compressions until advanced life support arrives. Depending on the location, this arrival time would most likely be longer than the two minute standard …

A Spatial Analysis Of Changes In Recreational Fishing Pressure On The Central Coast Of California Subsequent To Mpa Implementation, Morgan Ivens-Duran

Master's Theses

Marine Protected Areas (MPAs) are implemented to address a variety of management concerns, including conservation and restoration of fisheries, but few studies assess how MPAs affect regional fishing patterns. Previous research suggests effort will intensify at MPA edges, but few datasets include sufficient pre-implementation data to quantify how MPAs alter fishing effort. We used recreational fisheries data collected by scientific observers aboard Commercial Passenger Fishing Vessels that target nearshore fish species, primarily rockfish. We assessed shifts in the spatial distribution of fishing effort over a 10-year period that includes pre- and post-implementation observations of the California MPA network along the …

The Effects Of Age, Gender, And Gender Role Ideology On Adolescents' Social Perspective-Taking Ability And Tendency, Kaitlin M. Flannery

Master's Theses

The current research examined gender and age differences in adolescents’ social perspective-taking (SPT), or the social-cognitive ability to infer another person’s thoughts and feelings. This study aimed to demonstrate that SPT Ability and SPT Tendency are distinct constructs that should be considered separately. The influence of youths’ gender role ideologies on SPT also was considered. In a sample of adolescents ranging from ages 12 to 17 (N=158), participants reported their tendency to take the perspective of a specific same-age, same-gender friend, and also were assessed in their ability to use SPT in a hypothetical context. The results indicated …

Contrasting Leaf Shapes Vary In Extent Of Solar Tracking: Revisiting Darwin's "Service To The Plant", Kerri Mocko

Master's Theses

1. Solar tracking affects leaf temperature through the amount of direct light intercepting the lamina. However, leaf shape also affects leaf temperature due to heat transfer across boundary layers, and no studies have addressed the effect of leaf shape on solar tracking and leaf temperatures in the field.
2. We compare solar tracking, leaf temperatures, photosynthetic rates and leaf longevity in two co-occurring species with contrasting leaf morphologies: Pelargonium lobatum has a large, shallowly lobed leaf and P. triste has a highly dissected leaf composed of many small leaflets.
3. Pelargonium triste tracked the sun more closely than P. lobatum, although P. …

The Effectiveness Of Tipback Mechanics For Correction Of Class Ii Malocclusion, Nandakumar Janakiraman

Master's Theses

Objective: The objective of this prospective study was to quantify the maxillary incisor and first molar changes and further, evaluate the Class II correction with tipback mechanics.

Methods: Lateral cephalograms of 16 adolescents (7 males, 9 females) taken at T1 (pre-tipback), T2 (after tipback) and T3 (after molar root uprighting) were evaluated. Average age of study subjects was 12.63 + 1.1 years. Wilcoxon signed rank test was performed to test the significance at T1, T2 and T3. P-value of

Results: The maxillary first molar distalized by 1.53mm with 6.65 degrees of distal tipping and extrusion of 0.86mm …

The Role Of Carotenoid Cleavage Dioxygenase 4 In Flower Color Of The Allopolyploid Brassica Napus, Leanne Denice Fogg

Master's Theses

Allopolyploids are formed by interspecific hybridization and whole genome duplication, with the resulting organism contains multiple distinct subgenomes in one nucleus. Subgenomic interactions result in massive genetic and epigenetic reconstruction, contributing to variable phenotypic traits noted in newly formed allopolyploids. To better understand these mechanisms in the context of molecular biology, evolution, and plant breeding, plant biologists study the model organism Brassica napus (farmed as canola or oilseed rape). With white-flowering and yellow-flowering progenitors, flower color phenotype of B. napus presents an opportunity to examine subgenomic interactions. CAROTENOID CLEAVAGE DIOXYGENASE 4 (CCD4) is known to play a major …

Proliferation, Migration, And Survival Of Cells In The Telencephalon Of The Ball Python, Python Regius, Thomas B. Bales

Master's Theses

Reptiles exhibit neurogenesis throughout the brain during adulthood. However, very few studies have quantified telencephalon-wide neurogenesis in adulthood, and no studies have performed these investigations in snakes. Quantifying neurogenesis in the adult snake is essential to understanding class-wide adult neurogenesis and providing insight into the evolution of this trait. The thymidine analog 5-bromo-2’-deoxyuridine (BrdU) was used to quantify cell proliferation, migration, and survival in the ball python (Python regius). First, to determine the proper dose of BrdU for injection we subcutaneously injected 50mg/kg, 100mg/kg, and 250mg/kg into 15 adult male P. regius. We found the 250mg/kg dose …

The Effects Of Various Frequencies Of Low-Level Mechanical Vibration On Retention In An Orthodontic Relapse Model In Mice, Amir H. Assefnia

Master's Theses

ABSTRACT

Objectives: The aim of this study is to study the effect of mechanical vibration on retention following orthodontic tooth movement (OTM) in a mouse orthodontic relapse model. We applied various frequencies of vibration to the maxillary right first molar during relapse, and monitored changes to the alveolar bone and molar root.

Materials and Methods: Thirty-four male CD1 mice were placed in 4 groups. The Control Group underwent OTM for 7 days. The other three groups received OTM for 7 days, followed by a relapse phase of 7 days. OTM was performed with a 10g Ni-Ti spring. The Relapse Group …

The Use Of Single Flap Approach Versus The Double Flap Approach In The Treatment Of Intraosseous Periodontal Defects In Combination With Recombinant Human Platelet-Derived Growth Factor-Bb And Synthetic Beta-Tricalcium Phosphate: A Randomized Controlled Trial - Interim Report., Eric C. Hebert

Master's Theses

No abstract provided.

Experimental Peri-Implant Mucositis In Humans. A Pilot Study, Jonah A. Barasz

Master's Theses

No abstract provided.

Immediate Versus Delayed Loading Of Two Unsplinted Implants Supporting A Locator™ Retained Mandibular Overdenture. A Randomized Controlled Study, Satoko O. Rubin

Master's Theses

Background: Implant supported mandibular overdentures (OVD) have been proposed as the gold standard for the treatment of edentulous mandibles. In addition, the use of immediate loading may reduce the treatment time and improve the patient comfort without jeopardizing implant success. Limited information is available on the clinical success of immediate loading of 2 unsplinted implants supporting a mandibular OVD. The purpose of this study was to evaluate clinically and radiographically the performance of 2 unsplinted implants supporting a mandibular OVD 12 months post surgical placement, using either an immediate or a delayed loading protocol.

Methods: Each patient received 2 implants …

Chemically Modified Proteins As Highly Stable Building Blocks For Functional Bionanomaterials, Marc J. Novak

Master's Theses

Enzymes are key molecular machines that catalyze biologically important chemical reactions. The use of these powerful catalysts has recently been investigated for more industrial applications such as bio-fuel cells, glucose sensors, and biomedical engineering purposes. The main limiting factor of these versatile macromolecules is the stability concerns under conditions of elevated temperature or unfavorable pH. Chemical modification via charge manipulation of one of the most widely used enzymes in industrial research, glucose oxidase, is conducted in this report to stabilize the functional structure and improve the catalytic longevity under thermally taxing conditions. Further enhancement in solution stability is achieved by …

Does Nutrition Education Influence Snack Choices Of Preschoolers?, Laura Joseph

Master's Theses

Objective: To determine the impact of a nutrition education program on preschoolers’ snack choices when offered one healthy (grapes) or one unhealthy food (cookies).

Methods: This pre/post test experimental study included children ages 3-5 years old (n=45) from one preschool. Each child within a classroom (10-20 children) received the same nutrition education program (All 4 Kids) intervention (9, 30 minute lessons) taught by one registered dietitian over 2-weeks. Pre and post assessments included a snack knowledge and preference questionnaire and an observed snack selection trial to test the impact of the intervention. Children’s height and weight were measured …