P23h Rhodopsin Accumulation Disrupts Synaptic Protein Levels In Rod Photoreceptors, Samantha Thompson

Graduate Theses, Dissertations, and Problem Reports (ETD)

Rod photoreceptors are specialized neurons located in the retina, the neural tissue that lines the back of the eye. Rod photoreceptors are necessary for dim light and peripheral vision through the photoactivation rhodopsin (Rho). Rho is synthesized in the inner segment of rods before being unidirectionally trafficked to the outer segment. In diseases such as retinitis pigmentosa (RP), Rho trafficking can be disrupted, leading to progressive rod cell death. In many cases of RP, Rho is mislocalized to the rod photoreceptor synapse (“spherule”). The impact of Rho mislocalization on spherule structure and protein organization is poorly understood. We hypothesize that …

Introduction Of Polar Functional Groups To Pyridinedipyrrolide Zirconium Photosensitizers Through Post-Synthetic Modifications, Marisa Nicole Tordella

Graduate Theses, Dissertations, and Problem Reports (ETD)

Photosensitizers are vital components to applications such as photoredox catalysis, photodynamic therapy, and various avenues of solar energy production. The abilities of transition metal photosensitizers containing precious metals have been well studied over the years. However, because of the growing scarcity of these metals, there has been a shift in finding more earth abundant alternatives. To help fill this gap in the literature, the Milsmann group has developed and studied the photophysical properties of Zr(IV) complexes with various ligand systems. Due to the electron deficient metal center, these complexes exhibit ligand-to-metal charge transfer (LMCT), unlike the metal-to-ligand charge transfer commonly …

Simulated Slot Machine Gambling In Relation To Sign-Tracking, Polina Krom

Graduate Theses, Dissertations, and Problem Reports (ETD)

The propensity to attribute incentive salience to reward-related cues, or sign-tracking (ST) has been linked to addiction in animal models and some initial human data. The current study explores the role of sign-tracking (ST), as a potential risk factor for problematic slot machine gambling, which abundantly features audiovisual reward-related cues, henceforth sensory features. A sample of healthy participants without gambling problems (n = 30) completed a Pavlovian-to-Instrumental Transfer (PIT) paradigm to assess ST propensity and played on a realistic slot machine simulator consisting of two versions: with sensory features (SF+) and without (SF-). Each game version had fixed bet sizes, …

Not Far Enough: The West Virginia Human Rights Commission And The Civil Rights Movement, David Solomon

Graduate Theses, Dissertations, and Problem Reports (ETD)

This thesis investigates the West Virginia Human Rights Commission and its action surrounding the Civil Rights Movement. The Commission represented the liberal ideals that led white moderates to be potent allies of the Civil Rights Movement. Throughout the years following its inception, the Commission investigated and documented many instances of Jim Crow that were crushing Black communities in West Virginia. However, the limits of its liberal approach prevented it from creating solutions that would address the power structures that supported discrimination in West Virginia. By the late 1960s, Black frustrations with the lack of meaningful change boiled over into riots …

From Sequencing To Conservation: Genomic Analysis Of Three Sportfish Species In West Virginia, Andrew Johnson

Graduate Theses, Dissertations, and Problem Reports (ETD)

Situated in the Appalachian Mountains, one of the oldest mountain ranges on Earth, West Virginia waters boast rich ichthyofauna including native sportfish species walleye (Sander vitreus), largemouth bass (Micropterus nigricans), and muskellunge (Esox masquinongy). These three species are all native to the contemporary Ohio River watershed and play a major recreational and ecological role in local fisheries. Ecologically, all three species are apex predators and play a key role in ecosystems by directly influencing local fish assemblages. As dominant apex predators all three species are highly sought after in recreational fisheries with largemouth bass …

Mineralogical And Geochemical Assessment Of Rare Earth Element And Other Critical Mineral Occurrences In Coal-Derived Materials For Resource Recovery Potential, Sai Hemanth Kancha

Graduate Theses, Dissertations, and Problem Reports (ETD)

Rare earth elements (REEs) and other critical minerals (CMs) are indispensable to modern clean energy, electronics, and defense sectors due to their unique physical and chemical properties. The increasing global demand for these elements—combined with concerns about supply chain disruptions and the environmental impact of conventional mining—has heightened the urgency to identify alternative, sustainable sources. Among various secondary resources, coal-derived wastes such as coal refuse, fly ash, bottom ash, and coal acid mine drainage sludge have emerged as promising feedstocks for REE and CM recovery within the United States.

In this study, a comprehensive mineralogical and geochemical assessment was performed …

Repeatability And Reproducibility Of Minutiae Mark-Up, Lyrek Lockley

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study investigated the repeatability and reproducibility of minutiae mark-up. The use of fingerprints as a mode of identification has been around for thousands of years with contributions from figures like Sir William Hershel, Thomas Taylor, Henry Faulds, Sir Francis Galton and many more. Latent fingerprint impressions can be deposited by the perpetrator of a crime on a variety of surfaces at a crime scene. Many times, fingerprints are the most widely recovered and used evidence from a crime scene. Once fingerprints are collected, they go through the analysis, comparison, evaluation, and verification (ACE-V) process. This study will focus on …

Communicated Narrative Sensemaking In The Grandparent-Grandchild Relationship, Madison Clara Martin

Graduate Theses, Dissertations, and Problem Reports (ETD)

Storytelling is a primary form of communication between grandparents and grandchildren that has been shown to strengthen relational bonds and enhance grandchildren’s well-being. However, the specific narrative features and interactional processes involved in grandparent-grandchild storytelling remain underexplored, particularly regarding how they influence well-being and perceptions of generational differences. Guided by Communicated Narrative Sensemaking (CNSM) theory, this study examined the content (story framing) and process (interactional sensemaking behaviors) of grandparent-grandchild storytelling episodes as reported by young adult grandchildren (N = 110). Participants completed an online survey in which they recounted a storytelling episode with a grandparent and reported on individual well-being …

Discovering Appalachia As A Political Place: The Influence Of Appalachian Residence And Identification On Presidential Approval And Environmental Policy Preferences, Michael Bancroft Jones

Graduate Theses, Dissertations, and Problem Reports (ETD)

From poets to political scientists, yearning for a sense of place has motivated both song and research. Appalachia is an understudied place in political science, with previous research inconclusive as to whether the Appalachian region has a separate identity from other parts of the United States (White 2019). Identities may be derived from a sense a place. Alternatively, identities may develop from a sense of group identification with common interests and grievances, and concerns regarding out-group members. Appalachia has a long history of being different and set apart from the United States; and also, has been subjected to identity mobilization …

Reclaiming Revolutionary Relics: How Nineteenth-Century Americans Used George Washington’S Objects To Understand The Civil War, Abbi E. Smithmyer

Graduate Theses, Dissertations, and Problem Reports (ETD)

By combining cultural, memory, and material culture studies, this dissertation examines the ways objects associated with George Washington became instruments within the proxy war over whether the United States or the Confederacy best represented the American Revolutionary era’s principal values of liberty and equality. Exploring monuments, statues, historic homes, relics, and mementoes, as well as antebellum speeches and schoolbooks, letters, diaries, military orders, newspapers, and memoirs, this work demonstrates how the relics linked to Washington and the nation’s founding shaped how the Civil War generation thought about America’s past.

The 250th anniversary of the founding of the United States has …

Assessing And Predicting The Impact Of A Retrofitted Methane Mitigator On Non-Road Large Natural Gas Engines Using Artificial Neural Networks, Augustine Thomas Ojo

Graduate Theses, Dissertations, and Problem Reports (ETD)

Abstract

ASSESSING AND PREDICTING THE IMPACT OF A RETROFITTED METHANE MITIGATOR ON NON-ROAD LARGE NATURAL GAS ENGINES USING ARTIFICIAL NEURAL NETWORKS

Augustine Thomas Ojo

Methane emissions from the oil and gas sector present a pressing environmental and economic challenge, given methane’s high global warming potential and the considerable volumes of unutilized gas lost from industrial processes. This study investigates the impact of a retrofitted methane mitigation system, the Methane Mitigator (M²), on fuel consumption and engine-out emissions in non-road, four-stroke natural gas-powered, lean-burn engines and develops predictive Artificial Neural Network (ANN) models to estimate the resulting engine-out emissions and fuel …

The Dna Methylation Capacity Of The Tsetse Fly: Glossina Morsitans, And Its Integration With Symbiont Nutrient Provisioning, Dylan Forrest Richmond

Graduate Theses, Dissertations, and Problem Reports (ETD)

Tsetse flies (Diptera: Glossinidae) are the vector of trypanosomes (Trypanosoma spp.), which are responsible for African trypanosomiasis, also known as sleeping sickness. African trypanosomiasis is a significant health and economic burden yet remains a neglected tropical disease. As an obligate blood feeder, tsetse flies are reliant on B vitamin supplementation from intracellular Gammaproteobacteria, Wigglesworthia glossinidia. Folate (B9) is essential for tsetse development and reproduction, as well as DNA methylation in animals. As a reversible modification, DNA methylation typically occurs in a CpG dinucleotide context and can alter gene expression via the restriction of transcription. Across insects, the …

Development Of A Mixed Reality Robotics Testing Environment, Haneen Hanoon

Graduate Theses, Dissertations, and Problem Reports (ETD)

Development of a Mixed Reality Robotics Testing Environment

Haneen Hanoon

This research presents the development of a Mixed Reality (MR) robotic framework that integrates real and virtual environments for experimental robotics, education, and visualization. The proposed system combines a motion-capture system, a simulator, and a projector to create a spatially aligned, interactive workspace where physical robots coexist with projected virtual objects. The framework enables real-robot motion to be tracked in real time and mirrored in the simulated environment, allowing projected obstacles, goals, and paths to be precisely aligned with the physical world.

A coordinate transformation and scaling process was implemented …

Evaluation Of Small Unmanned Tailsitter Hybrid Air Vehicles Via Full Flight Simulation, Andrew Spencer Winters

Graduate Theses, Dissertations, and Problem Reports (ETD)

Small Unmanned Aerial Vehicles have exploded in popularity in the past 15 years among researchers and for industrial uses such as agriculture, search and rescue, and infrastructure inspection. Fixed wing and multirotor designs present two major paths that can be taken with these technologies. Fixed wing platforms have superior aerodynamic efficiency but require large areas for takeoff/landing and cannot hold a constant position in space, limiting their use. Multirotor configurations offer a more versatile platform that can maneuver in 3 dimensions independently but lack the endurance of fixed wing vehicles. This thesis analyses a quadrotor biplane and a variable wingspan …

Pancreatic Cancer In Appalachia: An Examination Of Detection, Associated Factors, And Provider Experiences With A Focus On West Virginia, Melissa Mae Elder

Graduate Theses, Dissertations, and Problem Reports (ETD)

ABSTRACT

Pancreatic Cancer in Appalachia: An Examination of Detection, Associated Factors, and Provider Experiences with a Focus on West Virginia

Melissa M. Elder

Introduction: The Appalachian population accounts for a substantial proportion of the United States and are uniquely susceptible to health care disparities. The Appalachian Regional Commission has reported on the inequities found within the Appalachian region as part of its formal establishment in 1965. When compared to the Non-Appalachian U.S., Appalachia experiences a higher incidence of chronic disease such as diabetes, cancer, lung, and liver disease; mortality from diseases of despair constituting prescription drug, alcohol, illegal drug use, …

Exploring The Ruminal Microbiome And Its Impacts On Feed Efficiency In Beef Cattle, Taylor Simone Sidney

Graduate Theses, Dissertations, and Problem Reports (ETD)

The ability for beef cattle to utilize feed efficiently is a critical determinant for both economic and environmental sustainability in livestock production. However, despite extensive research, the complex microbial ecosystem within the rumen and its relationship to feed efficiency remain incompletely understood. This dissertation explores the physiological and microbial foundations of feed efficiency by characterizing how rumen microbial communities and their functions differ among beef cattle with divergent residual feed intake (RFI) phenotypes and how the RFI status of beef cattle influenced their response to dietary interventions such as supplementation with a Bacillus-based feed additive. In the first study, …

Parameter Estimation Of A 100 Seater Simulated Passenger Aircraft, Michael James Winston

Graduate Theses, Dissertations, and Problem Reports (ETD)

Virtual flight test aircraft provide good opportunities to test new parameter estimation methods. However, good estimates for the parameters in VIRTTAC Castor, a virtual test aircraft, are unavailable. This thesis uses two well-established time domain methods, the Output Error Method and the Filter Error Method, to estimate the parameters of VIRTTAC Castor. Three standard maneuvers were used: Elevator 3-2-1-1, Rudder Doublet, and Aileron Multi-Step. The parameters were estimated across three different flight conditions: weight, altitude and air speed, which resulted in 27 tests for each maneuver. All parameters, and standard deviations estimated are included in the appendix as a table.

Development Of A Model To Estimate Engine Crankcase Methane Vent Emission Rates, Juan Pablo Rincon

Graduate Theses, Dissertations, and Problem Reports (ETD)

The primary focus of this research is the modeling and validation of crankcase methane emissions stemming from natural gas combustion engines commonly deployed in the natural gas compression industry. The models employed rely on equations predicting the flow rate and composition of losses, typically generated within reciprocating piston-cylinder systems. Since crankcase emissions originate in a piston–cylinder arrangement, multiple variables were considered in this study, including displaced volume, pressure, temperature, flow, and viscosity (with corrections applied to account for the thermal and compositional effects on the mixture of fluids). The initial data for this research was collected during three measurement campaigns …

Comparative Analysis Of Chemical Compounds And Metal Contaminants In Medical Grade Versus Over-The-Counter Sodium Hypochlorite Solutions, Abdulrahman Essaedi

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction:

Abstract

Endodontic treatment aims to prevent or address apical periodontitis by reducing intracanal bacterial populations to promote tissue healing, requiring thorough debridement and disinfection of the canal space. This study aims to examine the presence of trace metal elements and chemical compounds in over-the-counter (OTC) and medical-grade sodium hypochlorite (NaOCl) solutions.

Method

Over-the-counter NaOCl brands (Clorox® and Great Value®) and medical grade 6% NaOCl solutions (CanalPro® and Chlor-Xtra®) were purchased, stored in amber glass bottles, and tested in triplicate. Each was analyzed using mass spectrometry, identifying 7881 chemical compounds with statistical comparisons via adjusted p-values, fold-change, and heat maps. …

Short Episodes But Fast Friends: Examining The Effects Of Parasocial Relationship Quality On Mediated Intergenerational Contact In Grace And Frankie, Alysse C. Baker

Graduate Theses, Dissertations, and Problem Reports (ETD)

Building on the foundations of intergroup contact theory (Allport, 1954), the parasocial contact hypothesis (Schiappa et al., 2005), and Harwood’s Contact Space (2010), this dissertation advances the existing literature on how intergenerational contact with media characters can improve young adults’ willingness to engage with older adults. Specifically, this study examines the effects of intergroup contact quality on the parasocial contact process. Episodes of the TV sitcom about older adult women, Grace and Frankie, were edited to create both a high contact quality condition and a low contact quality condition. The high contact quality condition was a montage that showed viewers …

Cleaning And Characterization Of Chemical Vapor Deposited Graphene For Nanoelectronic Device Development, Sakib Ishraq

Graduate Theses, Dissertations, and Problem Reports (ETD)

Nanoelectronic devices based on graphene are a promising technology that combines the sensitivity and specificity of ion and element recognition with the accuracy and precision of electronics. The detection principle is based on the interaction of the target molecules with the nanodevice surface, which generates a measurable electrical signal. In the current study, graphene and its derivatives, synthesis and fabrication of high quality, good uniformity, and low defects graphene have been investigated as they are critical for high-performance and highly sensitive devices. Among many synthesis methods, chemical vapor deposition (CVD), have been used that needs to be transferred from the …

Biology And Ecology Of Neonectria Magnoliae And Neonectria Punicea, Two Understudied Neonectria Species In West Virginia, Hannah Marie Petronek

Graduate Theses, Dissertations, and Problem Reports (ETD)

The family Nectriaceae includes numerous phytopathogenic fungi that cause canker diseases on both angiosperm and conifer hosts worldwide. Numerous Neonectria spp. cause cankers on hardwoods and conifers in North America, but their roles in contributing to tree decline and mortality outside of beech bark disease are largely understudied. One such pathogen, Neonectria magnoliae, causes perennial cankers on two native hosts in central Appalachia: Fraser magnolia (Magnolia fraseri) and tulip-poplar (Liriodendron tulipifera). We also recently confirmed N. magnoliae from non-native star magnolia (Magnolia stellata) in West Virginia. Both native hosts occur in the central …

Influence Of Scaling And Root Planing With Minocycline Microspheres On Clinical Outcomes Of Patients With Periodontitis, Nupoor Sandeep Kulkarni

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Scaling and root planning (SRP) has been established as a “gold-standard” non-surgical initial treatment for periodontitis. Along with SRP, some systemic adjuncts like systemic antibiotics were used in the past to gain maximum benefits of SRP by reducing the need for further periodontal surgeries. Due to some drawbacks of systemic antibiotic use, like “transient antibiotic resistance”, “non-adherence” and “insufficient concentrations in the periodontal pockets to be clinically effective”, there was a shift towards the use of local antibiotic adjuncts to SRP like minocycline, tetracycline, chlorhexidine etc. Minocycline is a semi-synthetic tetracycline derivative which is available in a powder form, …

Three Essays In Urban And Regional Economics, Bryan Khoo Wyng Wai

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation "Three Essays in Urban and Regional Economics" contains three chapters which explore urban and regional economics. The first chapters focuses on examining the effect of stadiums on congestion; the second chapter on the effect of military base closures on the housing market; and the third chapter is a meta-analysis on historical instrumental variables.

The first chapter examines the effect of National Football League games on traffic congestion in Cincinnati, Ohio; Seattle, Washington; and Santa Clara, California during the 2018 and 2019 seasons. By linking sporting events to traffic congestion, this paper provides the mechanism for stadiums generating additional …

Enhancing Pipeline Simulations Through Artificial Intelligence And Machine Learning: A Smart Proxy Modelling Approach, Afeez Shittu

Graduate Theses, Dissertations, and Problem Reports (ETD)

ABSTRACT

Enhancing pipeline simulations is essential for improving operational efficiencies and effectively managing risks in the oil and gas industry. Traditional pipeline simulators, relying heavily on mathematical modeling assumptions, often face limitations due to their high energy and computational demands. This thesis addresses these challenges by introducing an innovative approach that integrates artificial intelligence (AI) and machine learning (ML) through a smart proxy model, offering a more efficient, cost-effective, and flexible alternative to conventional full-physics models used in pipeline simulation software.

The primary aim of this research is to develop and implement a smart proxy model capable of accurately predicting …

Fluid-Rock And Fluid-Steel Interaction In Geothermal Energy Development In The Appalachain Basin, Samuel H. Bowman

Graduate Theses, Dissertations, and Problem Reports (ETD)

The ever-increasing demand for clean energy requires creative solutions. In the United States, unconventional energy sources such as geothermal are conventionally limited to the western half of the country. The Appalachian Basin in the Eastern United States is one such possible creative solution, although the comparatively low temperature gradient presents a significant challenge to delivering economically feasible geothermal energy. An emerging technology is enhanced geothermal systems, these are engineered reservoirs where a working fluid (typically water) is cycled between the subsurface energy source and the energy collection facility at the surface. The liquid water interacts with the geothermal reservoir rock …

Implementation Of Provider In Triage Guidelines To Improve Emergency Department Throughput: A Quality Improvement Project, Benjamin Adam Klos

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction/Background

Emergency department overcrowding is a national problem that causes adverse effects on patients, providers, and healthcare systems. Locally, wait times can be up to six hours. Emergency department census increases yearly without an increase in physical space.

Purpose

The project aimed to improve throughput and provide quality care to patients in an emergency department waiting room.

Intervention

A provider in triage guideline was written to improve emergency department throughout metrics and guide care.

Methods

Pre- and post-implementation statistics were compared. The Logic model was used to guide project implementation.

Results

Door to provider and arrival to discharge times decreased …

Comparing Resurgence Following Positive And Negative Reinforcement Using A Human-Operant Approach, Abbie Cooper

Graduate Theses, Dissertations, and Problem Reports (ETD)

Resurgence is a type of relapse that consists of the recurrence of a previously eliminated response following worsening reinforcement conditions for an alternative response. Resurgence can occur following a history of positive or negative reinforcement; however, no previous evaluations have directly compared resurgence following these processes. In the present set of experiments, college students responded on a computer program to earn points (positive reinforcement) and to avoid losing points (negative reinforcement). Experiment 1 evaluated resurgence when both target and alternative responses were maintained by the same reinforcer class (i.e., in the positive-reinforcement component the target and alternative responses were positively …

Clinical Significance Of Gutta Percha Variability, Alex R. Pancake

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction: The production of gutta percha points as root canal therapy filling materials vary from hand processing to machine processing with a range of acceptable levels of error (tolerance). Similarly, endodontic files manufactured to complement corresponding gutta percha points exhibit specified levels of tolerance. Investigations into the consistency among manufacturers of files and gutta percha points is limited. The aim of this study is to see how variability in apical gauge and taper of gutta percha points affected the clinical performance during cone try in. The null hypothesis of this study is that there is no difference in two gutta …

Building Better Biofuel Crops: A Field Evaluation Of Transgenic Populus Modified To Improve Feedstock Performance For Bioenergy Use, Molli Elisabeth Coombs

Graduate Theses, Dissertations, and Problem Reports (ETD)

A transition away from fossil fuel use is necessary for the future of energy production, and biofuels are a renewable energy option that can mitigate the impacts of fossil fuels through lowered carbon emissions and possible carbon sequestration. In order to successfully compete with fossil fuels, the production of biofuels must be sustainable and cost effective. Populus species are a biofuel crop used for bioenergy conversion from lignocellulosic biomass that have been proposed for use in feedstock plantations on marginal lands, which would avoid competition with agricultural production. Two challenges of feedstock production being faced are recalcitrance to chemical processing, …