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Inactivation Kinetics Of Escherichia Coli And Listeria Monocytogenes In Edible Insects, Precious Aduloju

Graduate Theses, Dissertations, and Problem Reports (ETD)

Edible insects are emerging as sustainable and nutritious alternatives to conventional protein sources. However, concerns about food safety, particularly the presence of pathogenic bacteria such as Escherichia coli and Listeria monocytogenes, remain a barrier to consumer acceptance and industrial use. This study evaluated the effectiveness of thermal processing in inactivating E. coli, L. monocytogenes, and their non-pathogenic surrogates (E. coli JM109 and Listeria innocua) in both fresh and dehydrated forms of Tenebrio molitor (mealworm) and Acheta domesticus (cricket). It also evaluated the influence of insect species, moisture content, and temperature on microbial inactivation. A microbial challenge …

Genomic Differences And Parentage In West Virginia's Reintroduced Elk (Cervus Canadensis), Adam A.V. Cook

Graduate Theses, Dissertations, and Problem Reports (ETD)

Elk (Cervus canadensis) reintroductions have been common in the 20th and 21st centuries, yet 40% have failed to establish self-sustaining elk populations due to a lack of post-reintroduction demographic monitoring. Elk were reintroduced to West Virginia in 2016 following more than a century of absence after the species was extirpated in 1875. Elk were translocated to West Virginia from Land Between the Lakes National Recreation Area (LBL) in eastern Kentucky and from Arizona. The two groups of elk differ in their subspecies, physiology, reintroduction history, connectivity, and habitat. Reintroductions create genetic risks such as the founder effect and …

Mechanisms Of Neurovascular Dysfunction In Chronic Ischemic Stroke: Insights From Sex And Aging, Briana L. Clary

Graduate Theses, Dissertations, and Problem Reports (ETD)

Ischemic stroke is the leading cause of long-term disability world-wide. These disabilities can include both cognitive impairment and sensorimotor deficits. Due to the prevalence of post-stroke functional deficits, it is imperative to identify novel therapeutic targets and develop new treatments. These cognitive deficits are a result of damage to the neurovascular unit (NVU), a term used to describe the relationship between the cerebral vasculature and other cell types within the brain. Cognitive impairment as a result of damage to the NVU is referred to as vascular contributions to cognitive impairment and dementia (VCID), and it is estimated that between 25-30% …

Improving Hospital Discharge Readiness Among Caregivers Of Children With Complex Medical Needs, Megan M. Bruce

Graduate Theses, Dissertations, and Problem Reports (ETD)

Advancements in medicine and technologies have led to an increase in the population of children living with complex and chronic health conditions. Children with medical complexities (CMCs) and their caregivers frequently have challenges navigating home life after transitioning from hospital to home-based care. A 14-week doctoral capstone project was conducted at Dayton Children’s Hospital to improve caregiver readiness for hospital discharge. Project participants included caregivers of CMCs (n = 6) admitted to the hospital and pediatric acute care occupational therapists (n = 4). The Pediatric Readiness for Hospital Discharge Scale – Long Form (Peds-RHDS) was used as a pre-and post-intervention …

Mathematical Contributions To The Study Of Chemotaxis And Cell Signaling, Hajr Zam

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation presents results from two mathematical projects concerned with the biology of cells. Chapter 1 provides biological background and places the two mathematical problems in the context of cell signaling. The larger project, with Prof. H. Hattori on a chemotaxis model is presented in Chapters 3 and 4. Work with Prof. \'{A}. Hal\'{a}sz on a chemical reaction network system with linear multimers and two types of labels is presented in Chapter 2. The chemotaxis system describes the one-dimensional dynamics of a species of cells with two chemical species, a chemo-attractant and chemo-repellent. The goal is to analyze the behavior …

Phylogenomics And Biogeography Of Zinniinae (Asteraceae: Heliantheae) Using Plastome And Nuclear Datasets, Samuel Victor Skibicki

Graduate Theses, Dissertations, and Problem Reports (ETD)

Zinniinae is a subtribe of plants in the daisy family (Asteraceae) and the sunflower tribe (Heliantheae). Traditionally, seven genera were included in the subtribe: Echinacea, Heliopsis, Philactis, Sanvitalia, Tehuana, Trichocoryne, and Zinnia. The subtribe is poorly understood from a phylogenetic and taxonomic standpoint, and I undertook a phylogenetic investigation of Zinniinae, using both the plastid genome (plastomes) and the nuclear genome, to learn more about the evolutionary history of this clade. Plastomes were assembled to build a phylogenetic tree, targeted sequence capture was used to compile the nuclear datasets, and a chronogram was created to estimate date ranges for …

Late Ordovician Evolution Of Sea Scorpions: Eurypterids Of The Big Hill Lagerstätte, Joseph Donaldson Bingham Jr.

Graduate Theses, Dissertations, and Problem Reports (ETD)

Eurypterids are a group of diverse chelicerates that first appeared during the Middle Ordovician (467.3 Ma) and went extinct at the end of the Permian (251.9 Ma). The fossil record of eurypterids during the Ordovician is relatively sparce, comprising only 12 species representing the Megalograptidae, a ‘waeringopterid’ clade, Rhenopteridae, Onychopterellidae, Adelophthalmoidea, and Eurypteroidea clades. This study describes early eurypterids from the Katian aged (~452 Ma) Big Hill Lagerstätte of Michigan. The described material represents two new species, herein named Machipterus margaritae sp. nov. (based on 21 specimens preserving the ventral prosoma and prosomal appendages, and opisthosoma) and Dikranopterus lysinae sp. …

Impact Of Whey And Soy Protein Supplementation On Resistance Training In Young Adults: A Systematic Literature Review And Meta-Analysis, Brandon Eric Davis

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Whey protein is a popular supplement in sports nutrition. Soy protein is an alternative to whey protein, often marketed to individuals following a plant-based diet. Evaluating whey and soy protein supplementation's role in resistance exercise is essential for providing evidence-based nutrition guidance.

Objective: The study aim was to conduct a systematic literature review and meta-analysis on randomized control trials (RCTs) on the effectiveness of whey and soy protein supplementation on outcomes of lean body mass (LBM), squat, bench press, and plasma essential plasma amino acid (EAA) levels in young adult men and women.

Methods: The databases …

Comparison Of Compositional Characteristics Of Protein Extracted From Spent Hemp Seed Cakes By One-Step Defatting With Organic Solvents Followed By Isoelectric Solubilization Precipitation Using Strong And Weak Acids, Miah Nicole Gathman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Hemp seed cakes (HSC) are the byproduct of pressing hemp seeds for oil and are a significant agricultural waste product, with hundreds of tons produced annually. These nutrient-rich cakes have potential for sustainable applications in animal feed and food products. However, they could offer even greater value to the food industry through further extraction of the lipids and separation of the proteins they contain. Protein is a key nutritional component and companies are seeking cost-effective, time-efficient processes to concentrate protein from underutilized resources like HSC. Hexane (HEX) is a common solvent used in one-step defatting processes but may cause issue …

The Characterization Of Il-27 Production And Its Impact On T Cell Antigen-Specificity And Diversity In A Neonatal Bcg Vaccination Model, Ashley M. Divens

Graduate Theses, Dissertations, and Problem Reports (ETD)

Tuberculosis (TB) is caused by Mycobacterium tuberculosis (Mtb), and it has plagued humans for most of recorded history. Despite strong efforts to prevent and treat TB, it remains the leading cause of death due to a pathogen and is most prevalent in certain regions of Asia and Africa. Currently, the only approved vaccine to prevent TB is the live-attenuated Bacillus Calmette-Guérin (BCG) vaccine. Most countries have a universal BCG vaccination policy, but the vaccine ultimately fails to be protective. The BCG vaccine is most effective in children and protects against disseminated TB, but protection wanes 10-15 years after vaccination causing …

Creative Energies: Unearthing The Role Of Romantic Ecocriticism, Nicole Marie Chambers-Stahl

Graduate Theses, Dissertations, and Problem Reports (ETD)

The literary field of ecocriticism has transcended the boundaries of Academia: excited to connect to their physical environments, many of these scholars are focusing on genres like place-based writing, rhetoric of energy policy, and representations of the Earth in film and media. Interdisciplinary by nature, ecocriticism has demonstrated clear potential in its ability to intersect with concentrations like the history of science, animal studies, and environmental justice.

However, as the language around global crises is heating up —“Today it is scientists, not prophets,” writes Tobias Menely in “‘The Present Obfuscation,’” “who speak the language of apocalypse” (478)—ecocritical scholarship is at …

Biomechanics Of Teeth Alignment Using Clear Aligners With Various Attachment Shapes And Orientations, Egon Mamboleo

Graduate Theses, Dissertations, and Problem Reports (ETD)

Clear aligners have emerged as the most popular and preferred method of treatment for patients with orthodontic malocclusions. This is greatly due to the comfort and aesthetically appealing factors when compared to fixed appliances . Clear aligners are either thermoformed or 3D direct-printed plastics that apply biomechanical forces to the surface of teeth to trigger tooth movement and bone remodeling process. Common Class I malocclusions with mild or moderate crowding can be treated with clear aligners alone. However, treatment of Class II and Class III malocclusions that require extraction of permanent teeth or correction of severe rotations, and teeth extrusions …

Selective Recovery Of Critical Minerals (Ni, Zn, Co, And Mn) From Acid Mine Drainage Utilizing Ion Exchange And Selective Precipitation Processes, Esteban Albeiro Cardenas Restrepo

Graduate Theses, Dissertations, and Problem Reports (ETD)

Critical minerals (CMs) are essential for U.S. national security and technological development. The U.S. heavily depends on imports of these minerals from other countries, making supply chain disruptions a likely scenario. In consequence, finding alternative sources to conventional mining for these elements has become a topic of interest in recent years. Acid Mine Drainage (AMD), once solely viewed as an environmental problem, has emerged as a potential alternative source for CMs. AMD is a naturally occurring process where surface and underground water interact with sulfur-bearing minerals in the presence of oxygen and microorganisms. This interaction causes the water to become …

Thermal Inactivation Of E. Faecium On Ground Poultry, Joseph Katz

Graduate Theses, Dissertations, and Problem Reports (ETD)

Chicken products in grocery stores are contaminated with Salmonella that is ranked number one as a foodborne pathogen, and causes about 1.35 million cases of foodborne illness, 26,500 hospitalizations, and 420 deaths annually. The objective of this study is to determine thermal kinetic parameters of Salmonella surrogate bacteria E. faecium in ground poultry as affected by different temperatures and salt concentrations. The raw chicken was sliced up into a 500 g batch with a total of 6 batches per replicate, ground up in the meat grinder followed by inoculation with E. faecium resistant to 200 ppm Nalidixic Acid. NaCl …

Quantitative Assessment Of Knee Joint Stability Using Inertial Measurement Units In Patients With Acl Reconstruction Performing Single-Legged Hopping Tasks, Haley Hartman-Mongold

Graduate Theses, Dissertations, and Problem Reports (ETD)

Purpose: The purpose of this study was to examine proximal tibial IMU accelerations between the involved limb and uninvolved limbs of individuals who have undergone ACL reconstruction performing the single-leg hop test battery and to correlate these results to deficits in quadriceps strength and distance hopped. Methods: Thirty-four subjects (19 male, 15 female; age = 20.5 ± 6.7 years; mass = 76.4 ± 15.8 kg; height = 1.74 ± .08 cm) who have undergone unilateral ACL reconstruction were recruited for this study. An isokinetic dynamometer was used to assess the knee extension peak torque on the involved and uninvolved limbs. …

Essays On Application Of Machine Learning To Financial Statement Fraud, Lin Xia

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation is composed of two studies, both of which revolve around the application of machine learning to financial statement fraud. The first study entitled, “Complementary Role of Algorithm and Theory for Financial Statement Fraud” investigates the nature of relationship of theory building research and machine learning algorithms. Recent research on financial statement fraud has seen an increase in the use of machine learning techniques. While some machine learning based studies build on variables from causal inference research, others claim that machine learning algorithms work better with raw accounting variables and thus cast doubt on the role of …

International Students’ Perceptions And Experiences Of Institutional Diversity Initiatives And Self-Authorship Attainment: A Mixed Methods Study, Sandra Ayivor

Graduate Theses, Dissertations, and Problem Reports (ETD)

Higher education institutions in the United States have responded to years of discrimination through several diversity initiatives. Although research has indicated the merits of such initiatives on student development, the outcomes are not uniform across all student populations. Moreover, student development discourses have often excluded the experiences of international students. Employing a convergent mixed methods approach, this study investigated the perceptions and experiences of international students at a flagship land-grant higher education institution in the Appalachian region of the United States regarding institutional diversity initiatives and their self-authorship based on some demographic characteristics (i.e., gender, age, marital status, religion, continent, …

Prioritizing Amd Impaired Watersheds For Watershed-Scale Restoration Using Multi-Criteria Decision Analysis, James Nicholas Marino

Graduate Theses, Dissertations, and Problem Reports (ETD)

Acid Mine Drainage (AMD) remains a significant environmental challenge in coal-mining and hard rock regions like West Virginia, degrading water quality and ecosystem health. Traditional site-specific remediation efforts often prove inefficient in addressing AMD at large scale. This study explores watershed-scale restoration as a more effective solution, leveraging centralized treatment facilities and multi-source pollution management. This study will follow Multi-criteria Decision Analysis (MCDA), specifically the Analytical Hierarchy Process (AHP), to develop a structured rubric for grading watersheds. The process involves identifying critical criteria, organizing them into a hierarchy, and generating weights for clusters of criteria through collaboration with decision makers. …

Media Power And Racial Representation: A Critical Discourse Analysis Of Football Player, Tyreek Hill’S Police Encounter, Paulina Serwaa Gyamfi

Graduate Theses, Dissertations, and Problem Reports (ETD)

This thesis critically examines how mainstream media outlets construct and perpetuate racial narratives through their coverage of high-profile incidents involving Black public figures. Using the case study of NFL player Tyreek Hill’s encounter with Miami-Dade police on September 8, 2024, the research employs Critical Discourse Analysis (CDA) and Critical Race Theory (CRT) to analyze news articles from six major outlets: The New York Times, NBC News, CBS News, ESPN, Fox News and CNN. The study explores how language choices, framing techniques, and contextual elements in media reporting influence public perceptions of race, celebrity and criminal justice. Findings reveal that while …

Xanthine Oxidoreductase, Uric Acid, And Iron Homeostasis: Discovering A Therapeutic Vulnerability In Breast Cancer, Matthew G. Chapa

Graduate Theses, Dissertations, and Problem Reports (ETD)

Breast cancer remains the most frequently diagnosed malignancy and the leading cause of cancer related deaths in women. This is primarily due to distant metastases, which highlight the necessity to understand factors that contribute to breast cancer progression. Emerging evidence associates decreased tumor xanthine oxidoreductase (XOR) expression with a poorer prognosis, increased recurrence, and a more aggressive phenotype. While healthy liver, intestines, kidneys, and breast tissue have robust XOR expression and activity, there is a tendency for this to be ablated in carcinogenesis. This dissertation investigates XOR's role in breast cancer and identifies its product, uric acid (UA), as possessing …

Influence Of Different Rates Of Biochar And Deer Browsing On Pollinator Plant Productivity, Bipana Bhusal

Graduate Theses, Dissertations, and Problem Reports (ETD)

Biochar, a carbon-rich material produced from biomass, has received wide attention for soil amendment to improve plant growth. However, its specific effects on pollinator friendly plants remain poorly understood. In this study, we evaluated the effects of four biochar application rates (0, 10, 20, 40 tons/acre) and deer exclusion (fenced vs. unfenced) on the productivity of three pollinator species; Buckwheat (Fagopyrum esculentum), Plains Coreopsis (Coreopsis tinctoria), and Black-eyed Susan (Rudbeckia hirta) in a utility right of way with a history of mining. A split-plot randomized design was used, and the results were analyzed using …

Impacts Of Trout Stocking And Non-Native Trout Populations On Wild Brook Trout Salvelinus Fontinalis In West Virginia Streams, Xander A. Lamping

Graduate Theses, Dissertations, and Problem Reports (ETD)

Understanding the extent that anglers targeting stocked trout end up harvesting wild Brook Trout Salvelinus fontinalis addition to studying how non-native trout species affect native Brook Trout populations is important for effective management and conservation in the central Appalachian Mountains. This thesis addresses these concerns in two parts. The first chapter used a combination of creel surveys and non-lethal morphometric methods to estimate wild Brook Trout harvest rates on four stocked streams during the West Virginia spring stocking season. A generalized linear model was developed to determine the origin (wild vs. hatchery) of Brook Trout harvested by anglers. This differentiation …

State Determinants Of Medicaid Fraud Convictions: Insights From Panel Regression Analysis, Kevin Patrick Krantz

Graduate Theses, Dissertations, and Problem Reports (ETD)

Medicaid is the largest public health insurance program in the United States, providing critical coverage to over 78 million low-income individuals, children, and people with disabilities. While Medicaid fraud has received extensive public and political attention, empirical research examining enforcement outcomes across states remains limited. This study analyzes state-level Medicaid fraud metrics from 2010 to 2022 to evaluate how structural and policy changes, particularly rising managed care enrollment and the implementation of the 2010 Affordable Care Act and the 2016 Managed Care Final Rule, have shaped enforcement outcomes. Using random-effects generalized least squares (GLS) and fixed-effects panel regression on a …

Microbial And Plant Biodiversity In Appalachian Agroecosystems And Their Implications For Soil Health, Kinsey Reed Close

Graduate Theses, Dissertations, and Problem Reports (ETD)

The sustainability of agricultural landscapes depends on understanding the complex interactions between plants, soil, and microbes. In Appalachia, a region shaped by complex terrain and long histories of human and ecological interaction, small family farms produce hay, livestock, and row crops. These agroecosystems, though often treated as “artificial” by many ecologists, have the potential to support both food production and biodiversity conservation if managed with an understanding of ecological complexity. This dissertation examines how plant and microbial diversity interact with soil and management to influence nutrient cycling, soil health, and long-term resilience in Appalachian agricultural systems. A literature review revealed …

Synergetic Effect Of Cellulose And Chitosan Polymers In Cancer Treatment, Grace Peterson

Graduate Theses, Dissertations, and Problem Reports (ETD)

This research explores the development of a biocompatible, natural drug delivery system designed for targeted cancer therapy. Lung cancer cells are used in these particular tests, but further research indicates treatment could be used for various cancer types. Traditional cancer treatments like chemotherapy are often limited by poor selectivity for tumor cells, causing significant damage to healthy tissues. The study proposes a dual-layered delivery system, utilizing Carboxymethyl Cellulose (CMC) as the core material and a charge-reversible chitosan shell to encapsulate and target cancer drugs. CMC, with its water solubility and ability to form stable gels, serves as a stable drug …

Sensory Evaluation And Consumer Acceptance Of A Protein Enriched Energy Bar, Brennah T. Groves

Graduate Theses, Dissertations, and Problem Reports (ETD)

ABSTRACT

Sensory Evaluation and Consumer Acceptance of a Protein Enriched Energy Bar

Brennah Groves

This study evaluated the sensory properties and consumer acceptance of a protein enriched energy bar. The objectives of this study were to 1) formulate a high-protein energy bar containing at least 20 g of protein per 100 g serving, and 2) determine whether increasing the protein content in an energy bar would enhance the sensory evaluation and improve consumer acceptance among collegiate athletes. The control energy bar featured a shortbread base, date caramel with whey protein, a nougat layer, and a chocolate coating. Additional collagen protein …

Microbiological Examination Of Yellow Mealworm (Tenebrio Molitor) Larvae Under Various Processing And Storage Conditions As Potential For Human Consumption, Rachel Midkiff

Graduate Theses, Dissertations, and Problem Reports (ETD)

Yellow mealworm (Tenebrio molitor) larvae have gained attention as a sustainable and nutritionally balanced food source, yet their high natural bacterial load raises food safety concerns. This study investigates the impact of pre-processing, processing, and storage conditions on microbial load and diversity in mealworms, comparing thermal (hot) and non-thermal (cold) treatments. These treatments include freeze-drying, wet heat treatments, dry heat treatments, and the combination of wet and dry heat treatments against untreated controls. Microbial loads were quantified by plate counts after day 0, day 10, and day 28 of vacuum-sealed refrigerated (4°C) storage. Water activity (Aw), pH, and moisture content …

Exploring Teacher Perception And Implementation Of Sae Programming In West Virginia, Joseph Laflamme

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this study was to describe the phenomenon of SAE implementation in West Virginia. This study utilized qualitative research methodology to describe teacher perceptions of how student, guardian, school and community factors affect SAE program implementation. Additionally, teacher backgrounds, including formative experiences, training and education, guiding philosophies, skills, and motivation were explored. This study describes contextual aspects of population, economics, state guidelines and local agriculture industries. This is done through a document review of student reported records as well as surveys and teacher interviews.

What Does It All Mean? Differences In Self-Defining Memories Between Community And Adjudicated Youth, Hannah Elias

Graduate Theses, Dissertations, and Problem Reports (ETD)

Identity development is driven in large part by self-defining memories, or memories though which we base our definition of who we are (Habermas & Bluck, 2000). In adolescence, cognitive skills are gained that allow us to make meaning, or learn lessons and gain insight, from self-defining memories (Bluck & Habermas, 2001; McLean & Thorne, 2003). To determine whether there are differences in their ability to make meaning from their self-defining memories, this study compares narratives of self-defining memories between community youth and adjudicated youth. Additionally, the ability to be specific in self-defining memory recollection may differ between these groups, which …

A Limb-Speed-Driven Locomotor Control System And Its Ability To Adapt, Emily Marie Herrick

Graduate Theses, Dissertations, and Problem Reports (ETD)

Despite how simple walking may seem, the locomotor control system is structurally and functionally complex. Its hierarchical organization of supraspinal and spinal networks with forward and feedback pathways has many interactions at multiple levels that are dependent on the dynamics of a high-dimensional musculoskeletal system. Having a comprehensive understanding of sensorimotor integration within a healthy locomotor control system is crucial for understanding changes to the system due to age or neurologic disease and developing effective technologies to recover mobility in those populations. In this dissertation, we address persistent gaps in knowledge pertaining to how the nervous system controls locomotion.

In …