Digital Commons Network^™

A Fine-Scale Genomic Study Of Cheat Mountain Salamanders (Plethodon Nettingi) On Managed Public Land, Amylynn Nicole Ephraim

Graduate Theses, Dissertations, and Problem Reports (ETD)

Habitat fragmentation has the potential to isolate localized populations, particularly those which are understood to have limited dispersal capabilities. This isolation can reduce genetic diversity through the effects of inbreeding depression, and small populations are more susceptible to the impacts of genetic drift. In this study, I aim to understand the level of genetic connectivity between known occupied patches of Cheat Mountain salamanders (Plethodon nettingi; CMS) on managed public land. CMS are a federally threatened, high-elevation, West Virginia endemic species associated with red spruce (Picea rubens) forests. I used double-digest restriction site associated DNA (ddRAD) sequencing …

Plant Invasion Patterns In Understory And Soil Microbial Communities Of North Central West Virginia Mixed Hardwood Forests, Isabelle Marie Uganski

Graduate Theses, Dissertations, and Problem Reports (ETD)

Invasive plants alter forest ecosystems by reducing species richness, habitat heterogeneity, and nutrient cycling as they rapidly displace native plants. Invasive plant composition can influence soil health through altering soil microbial associations and communities. By studying the mechanisms in which invasives alter above- and below-ground communities, we can better understand whole-ecosystem impacts and inform management decisions. This study examined the relationships between co-occurring invasion on above and belowground communities of mixed hardwood forests of north central West Virginia. Despite distinctive associations with aboveground characteristics (native diversity, native tree regeneration, and leaf litter density) we detected limited changes to soil microbial …

Comparative Evaluation Of Bending Resistance And Retentive Torque Stability Of Zest Locator® And Straumann Novaloc® Abutments: An In Vitro Study, Hammad Ul Haq

Graduate Theses, Dissertations, and Problem Reports (ETD)

Abstract

Comparative Evaluation of Bending Resistance and Retentive Torque Stability of Zest Locator® and Straumann Novaloc® Abutments: An In Vitro Study

Hammad Ul Haq B.D.S.

Objective: To compare the mechanical performance of Locator® (Zest Anchors) and Novaloc® (Straumann) implant overdenture attachment systems connected to Straumann Bone Level Conical (BLC) implants under standardized cyclic and static off-axis loading conditions.

Materials and Methods: Ten Straumann BLC implants were divided into two groups (n = 5): Group 1 received Locator® abutments and Group 2 received Novaloc® abutments. All abutments were tightened to 35 N·cm and retightened after 10 minutes. Baseline removal torque values …

Deep Learning-Based Co-Current Upward Gas-Liquid Two-Phase Flow Regime Identification In An Annular Conduit, Joshua Robert Macomber

Graduate Theses, Dissertations, and Problem Reports (ETD)

Flow regime identification in co-current upward gas-liquid flow through annular conduits remains a significant challenge in petroleum engineering, with major safety and operational implications. It is also important across industries involving the transport of multiphase fluids. Misidentifying flow regimes can introduce major operational risk, yet regime boundaries in annular gas-liquid flow are often visually complex and context dependent.

The objective of this study was to evaluate the utility of convolutional neural network (CNN) classifiers for flow regime identification. The CNN was trained using annular flow image dataset published by Texas A&M University. The dataset consists of approximately 947 RGB images …

Public Perception Of Using Public Lands For Climate Change Mitigation, Casey Marie Zaitzew

Graduate Theses, Dissertations, and Problem Reports (ETD)

Biological carbon sequestration provides a way to integrate carbon initiatives while meeting sustainable forestry management needs. In the United States, woody plants capture about 13% of annual carbon emissions, highlighting the importance of forests and other ecosystems in national climate strategies.With over 640 million acres of public land, management strategies that incorporate carbon sequestration could significantly influence national carbon storage. Because public lands are taxpayer-funded, public acceptance is critical in carbon-focused policies. While prior studies have examined public attitudes toward carbon capture and storage techniques, fewer studies have focused on biological sequestration within federally managed lands.

This study analyzes public …

Gcn5l1-Mediated Lysine Acetylation Regulates Mitochondrial Dysfunction In The Aged Heart, Jackson Edmond Stewart

Graduate Theses, Dissertations, and Problem Reports (ETD)

For over a century, cardiovascular disease (CVD) has been and remains the leading cause of death globally. CVD risk and severity increase significantly with advanced age, and mitochondrial dysfunction has been implicated in the pathogenesis of aging. Furthermore, mitochondrial health is crucial for cardiac function, as one-third of total cardiomyocyte volume is occupied by mitochondria in order to meet the high energy demand of contractile and relaxational function. It is thus crucial that we understand the pathological mechanisms driving mitochondrial dysfunction in aging and how this contributes to age-associated cardiovascular disease. The current manuscript aims to fill this knowledge gap …

The Dual-Factor Model (Dfm) Of Mental Among Appalachian Youth, Mawyah Adel Bashatah

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background. Adolescent mental health is a critical public health concern, particularly in rural Appalachian communities where rates of depression, anxiety, and substance use exceed national averages and access to mental health resources remain severely limited. Traditional mental health frameworks rely predominantly on the identification and reduction of psychopathological symptoms, neglecting the independent contributions of positive well-being to adolescent risk and resilience. The Dual-Factor Model (DFM) of mental health offers a more comprehensive alternative by simultaneously assessing subjective well-being (SWB) and psychopathology, classifying individuals into four distinct groups: Complete mental health, Vulnerable, Symptomatic but content, and Troubled. Despite the DFM’s growing …

Feasibility Study Of Concurrent Removal Of Organics And Ammonium Using An Iron Dosed Upflow Anaerobic Sludge-Blanket Bioreactor, Morgan Elizabeth Amos

Graduate Theses, Dissertations, and Problem Reports (ETD)

Aerobic microbial reactions have commonly been utilized in treatment processes to oxidize and remove organics and nitrogen through aeration, using processes such as nitrification that converts ammonium (NH₄⁺) into nitrite (NO₂^-) and nitrate (NO₃^-). Anoxic microbial reactions like denitrification often require organic compounds as an external source to chemically reduce the oxidized nitrogen (NO₃^- and NO₂^-) to nitrogen gas (N₂) for nitrogen removal from the liquid phase. These mainstream processes have a high chemical cost and energy footprint which results in an increased release …

Operationalizations Of Adverse Childhood Experiences And Health Prediction, Sarah Josephine Miller

Graduate Theses, Dissertations, and Problem Reports (ETD)

Exposure to adverse childhood experiences (ACEs) has been consistently linked to suboptimal health outcomes and behaviors across the lifespan. As such, there has been widespread adoption of measures like the 10-item cumulative ACEs count measure developed by Felitti and colleagues (1998) across research literature and clinical practice. However, this measure was not developed with consideration of optimal psychometric properties and thus consists of several limitations that inhibit understanding of the specific dimensions of adversity that influence health outcomes. This 3-study dissertation focused on different measurement and statistical techniques to better understand the associations between ACEs and health. In study 1, …

Differences In Dry Matter Intake In Grazing And Drylot Beef Cattle, Tylor John Yost

Graduate Theses, Dissertations, and Problem Reports (ETD)

Accurate measurement of individual dry matter intake (DMI) in grazing cattle remains a major challenge for evaluating feed efficiency traits in beef production systems. This study evaluated relationships among intake and efficiency traits measured in both pasture and drylot environments and assessed the potential for predicting pasture DMI using water intake and machine learning approaches. A total of 163 beef cattle across four experimental groups were evaluated in one of two grazing seasons (2024 or 2025). Drylot feed and water intake were measured using automated intake monitoring equipment, whereas pasture intake was determined using a long short-term memory …

Framing Addiction In The Age Of Vaping: A Journalism-Focused Reporting Toolkit, Jessica L. Riley

Graduate Theses, Dissertations, and Problem Reports (ETD)

This project addresses a critical gap in journalism practice: the lack of accessible, evidence-based guidance for reporting on addiction. Particularly as it relates to nicotine, tobacco and vaping. Journalists play a key role in shaping public understanding of substance use, yet existing resources often focus on opioids, lack specificity, or fail to keep pace with rapidly evolving nicotine products and scientific research. As a result, reporters may struggle with inconsistent terminology, unclear risk communication and the potential for unintentionally stigmatizing language.

Grounded in media effects theory, health communication and stigma research, this project examines how journalistic language and framing influence …

Multi-Omics Analysis Of Immune-Metabolic Adaptation To Direct-Fed Microbial Supplementation In Newly Weaned Beef Steers, Adekunle Odunayo Ajiboye

Graduate Theses, Dissertations, and Problem Reports (ETD)

Immune and metabolic disruption during weaning represents a major challenge to health and productivity in beef cattle. This dissertation tested the hypothesis that supplementation with multi-strain direct-fed microbials (DFMs) modulates immune regulation and metabolic pathways associated with adaptation to weaning stress. To address this, whole-blood transcriptomics and plasma metabolomics were used to evaluate molecular and metabolic responses in newly weaned beef steers during the receiving period. Transcriptomic analysis identified differential expressions of genes associated with immune signaling and inflammatory regulation, indicating modulation of immune-related pathways without changes in growth performance. Metabolomic analysis revealed alterations in circulating metabolites related to amino …

Computational Methods For Identification Of Molecular Signatures, Weijun Yi

Graduate Theses, Dissertations, and Problem Reports (ETD)

This work develops computational methods for identifying molecular signatures from high-throughput genomic data and for modeling long non-coding RNA (lncRNA) sub-cellular localization. The response of multiple myeloma to CB-6644, a selective RUVBL1/2 complex inhibitor with potential anti-tumor activity, is analyzed to identify drug-responsive pathways and molecular signatures. Conventional gene set enrichment analysis (GSEA) often excludes low-expression genes. Here, phenotype comparison is reformulated as a supervised machine learning problem: genes most informative for discrimination are first selected using a machine learning approach, and GSEA is then applied to these machine-learning derived gene sets. This framework improves detection of CB-6644-associated pathways. For …

Bridging Physics-Based Modeling And Machine Learning To Predict Material Behavior: Applications In Fatigue Crack Growth And Dielectric Property Characterization, Ansan Pokharel

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation integrates physics-based modeling with machine learning (ML) to predict how materials behave under complex thermal and mechanical conditions. A key innovation of this work is the use of finite element analysis (FEA) to supplement experimental data. This approach creates more diverse and representative synthetic datasets, helping to reduce the limitations and biases that arise when training ML models solely on experimental measurements. The research focuses on two applications: improving the prediction of fatigue properties in superalloys and estimating temperature-dependent, high-frequency dielectric properties relevant to microwave-based chemical processing.

In the first study, low-cycle fatigue experiments were performed on the …

A Prospective Analysis Of Success And Survival Of Haptic Robotically Driven Dental Implant Placement., Samuel Clarkman Smith

Graduate Theses, Dissertations, and Problem Reports (ETD)

Purpose: The aim of this prospective study was to assess survivability of dental implants placed using robotic computer aided implant surgery via Yomi® (Neocis, Inc. Miami, FL) after at least 1 year of functional loading. Secondary analysis included additional success and marginal bone loss outcomes after at least 1 year of functional loading of the same Straumann (Basel, Switzerland) titanium dental implants that were placed with the r-CAIS workflow. Materials and Methods: Since acquisition of the Yomi® robotic system, a comprehensive list of patients who underwent dental implant therapy via r-CAIS methodology at West Virginia University Department of Graduate Periodontics …

Using Side Looking Hydroacoustics And Community Size Spectra To Assess Invasive Carp’S Impact On Fish Assemblage Integrity, Daniel Jacob Bryan

Graduate Theses, Dissertations, and Problem Reports (ETD)

Often accounting for over half of total fish biomass, invasive silver carp have dramatically reshaped the ecosystem of the Mississippi River and its tributaries. These high silver carp densities have been shown to affect native fish body condition, abundance, and community structure, highlighting the need for efficient methods to quantify silver carp’s effect on the native fish assemblage. Community size spectra (CSS) is a metric that quantifies fish assemblage body size structure based on the decrease in fish abundance with increasing size. To estimate silver carp’s effects on CSS in the Ohio River, side looking hydroacoustic monitoring was used alongside …

Utilizing Plethodontid Occupancy To Assess Initial Ecosystem Responses To Invasive Plant Control Treatment In The Central Hardwoods, Maree Guinevere Dieterich

Graduate Theses, Dissertations, and Problem Reports (ETD)

Invasive plants are a growing concern for land managers, impacting ecosystem resilience and outcompeting native vegetation. To combat this, invasive plant control treatments can assist in reducing the impacts and spread of damaging, non-native plant species. However, intensive treatments completed at large scales have the potential to negatively impact ecosystem services as well. To monitor these impacts, I utilized a well-known indicator in the Central Applachian region, Plethodontid salamanders, to assess impacts of invasive plant control treatments in the Central Hardwood forests. I completed 12 visual samples across 60 sites in West Virginia, USA during Spring and Fall of 2025 …

Tree Crown Economics Of Broadleaf Deciduous Forests, Yiting Fan

Graduate Theses, Dissertations, and Problem Reports (ETD)

Tree crown architecture, a critical determinant of forest ecosystem processes such as photosynthesis, evapotranspiration, and spectral reflectance, is shaped by adaptive trade-offs in resource use and environmental responses. However, significant gaps remain in our understanding of how these traits vary across species, environmental gradients, and temporal scales. This dissertation addresses these gaps by employing remote sensing data across three interconnected studies. Together, these studies advance tree crown economic theory, highlighting how crown traits mediate trade-offs between light capture and water-use efficiency and how these traits influence forest responses to global change. Collectively, this dissertation offer insights for improving models that …

Free Energy Approaches To Understanding The Mechanism Of Membrane-Active Peptides, Hannah R. Scott

Graduate Theses, Dissertations, and Problem Reports (ETD)

Cancer is a leading cause of death in the world today. Current methods of treatment fail to distinguish the difference between cancer cells and cells of healthy tissue, leading to off-target effects, often with terminal outcomes. A potential solution to this challenge is to target the cancer cells’ microenvironment. One universal characteristic across all cancer cells is the acidic extracellular environment. The pH-Low Insertion Peptide (pHLIP) is a membrane-active peptide with acid-sensitive function, undergoing folding and insertion into a transmembrane alpha-helix. pHLIP is a promising candidate to be used to deliver anti-cancer agents exclusively to cancer cells. Although the mechanism …

Neural And Computational Approach To Understanding Environmental Modulation Of Behavioral Identity In Zebrafish, John W. Hageter

Graduate Theses, Dissertations, and Problem Reports (ETD)

Organisms rely on behavior for survival. Animals engage in behaviors that allow for feeding, mating, exploring and navigating their environment among others. Necessary for these behaviors to develop are the environmental factors and underlying circuitry which make behavior possible. Specifically, how the environment guides underlying neural circuitry to develop unique facets or phenotypes of a larger behavior are key to understanding why unique behaviors exist. In this thesis, I build foundational evidence for determining these mechanisms through the use of the zebrafish local search behavior. This is a behavior that zebrafish employ following the loss of environmental illumination where they …

Designing Into The Woods: An In-Depth Analysis, Jackson Berhow

Graduate Theses, Dissertations, and Problem Reports (ETD)

This thesis is intended to provide insight into the costume design process for West Virginia University’s production of Into the Woods. This thesis will document the entire process, starting with a close reading of the musical exploring its symbols, themes, and characters with some theory crafting thrown in for good measure. This will be followed by the early stages of the show’s production, as well as a full explanation of the development of each character’s design throughout the show’s conceptual evolution. The show’s production process will also be explored, highlighting notable instances and how this impacted the costume design.

Reprogramming Of Immunometabolic Functions In Brain Microvascular Endothelial Cells During Neuroinflammation: Insights From Ischemic Stroke And Sepsis, Shokofeh Rahimpourkaldeh

Graduate Theses, Dissertations, and Problem Reports (ETD)

Ischemic stroke and sepsis-associated encephalopathy (SAE) are two distinct conditions that share common pathophysiological mechanisms affecting brain microvascular endothelial cells (BMECs). Ischemic stroke results from an acute reduction in cerebral blood flow due to thrombosis or embolism, leading to energy failure, oxidative stress, and blood-brain barrier (BBB) dysfunction. SAE, a neurological complication of sepsis, arises from systemic inflammation, endothelial activation, and BBB disruption, leading to cognitive impairment and neuroinflammation. Despite their different etiologies, both conditions involve BMEC dysfunction as a central mediator of neurovascular injury. This dissertation aims to investigate the immunometabolic dysfunction of BMECs in ischemic stroke and sepsis, …

Skeletal And Dental Effects Of The Carriere Motion Appliance™ In Non-Growing Class Ii Patients: A Cephalometric Study, Anna Cathlyn Giles

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background and Objectives: Dental and skeletal Class II discrepancies are commonly associated with orofacial disharmony, and therefore appear in orthodontic clinics frequently. Class II correction for adults is generally limited to camouflage treatment with extractions, Class II elastics, Class II correctors, distalization, or orthognathic surgery in severe cases. The orthodontic field is constantly innovating to find efficient and effective means to correct class II malocclusions. The Carriere Motion Appliance TM was introduced in 2004 as an effective “distalizing” appliance to be used as a non-extraction approach to Class II correction for adolescent and adult patients. Recent clinical evidence suggests that …

A Post Apocalyptic Romp: Designing Costumes For Phoebe In Winter, Emmalee Berger

Graduate Theses, Dissertations, and Problem Reports (ETD)

This thesis will document the process of costume designing for Phoebe in Winter by Jen Silverman. It will examine the process from first read of the script through initial and final design meetings and to execution and production of the design. This will report on challenges specific to this production, such as nudity and violence on an academic stage, and the subsequent solutions. Phoebe in Winter was produced by West Virginia University School of Theatre and Dance in October of 2024. The design process began in February of the same year.

Psychophysiological Tools & Techniques To Predict Perceptual Motor Task Outcomes, Bobby Gerald Rawls Iii

Graduate Theses, Dissertations, and Problem Reports (ETD)

The process of perceiving environmental information, analyzing it, formulating a plan of action, and carrying out said plan is vital to our day to day lives. This loop occurs for every motor task we perform, from actions as simple as elbow flexion to as complex as performing surgery. As such, it is imperative to understand what physiological, psychological, and neurocognitive factors most dictate success or failure in the motor performance loop.

Prior research has shown that measures from each of these facets have strong relationships with motor performance outcomes, though these findings tended to rely on artificially produced stress in …

Therapeutic Roles For Alkaline Phosphatases To Target Intestinal Dysfunction In Mouse Models Of Experimental Sepsis And Ischemic Stroke, Rhiannon V. Macom

Graduate Theses, Dissertations, and Problem Reports (ETD)

Abstract

Therapeutic Roles for Alkaline Phosphatases to Target Intestinal Dysfunction in Mouse Models of Experimental Sepsis and Ischemic Stroke

Rhiannon V. Macom

Stroke is a disease that primarily affects the brain; however, ongoing research continues to show that stroke has far reaching systemic effects that can affect both short- and long-term recovery. The gastrointestinal tract has been shown to be affected post-stroke, and these effects are likely due to the bidirectional communication between the gut and the brain via the gut-brain-axis. Alkaline phosphatases (APs) are common membrane bound enzymes that are known to have a role in mitigating inflammation. The …

Understanding The Prevalence Of Γ-Hydroxybutyric Acid In Hair For Clinical And Forensic Applications, Collin J. Kustera

Graduate Theses, Dissertations, and Problem Reports (ETD)

Sexual assault affects over 400,000 Americans annually, including 13% of college students, yet only 2.5% of assailants face incarceration. This disparity stems from significant challenges in evidence collection, particularly in drug-facilitated crimes (DFCs). Victims often delay reporting due to fear, self-blame, or unawareness of the assault which is common in cases involving substances like γ-hydroxybutyric acid (GHB). Current forensic standards rely on blood and urine, which offer detection windows of just 48 and 120 hours, respectively. These short timeframes frequently fail to capture critical toxicological evidence, leaving investigators with only victim testimony, which the judicial system struggles to act upon …

Transforming The Athlete Through Occupational Therapy-Facilitated Mentorship Programming, Cecelia D. Simons

Graduate Theses, Dissertations, and Problem Reports (ETD)

There is a high prevalence of role strain experienced among collegiate athletes with increased attention and awareness surrounding its impact on the transition out of sport and into the next phase of their lives. Many colleges and universities throughout the United States have developed career readiness programs focused on mental health, career transitions, and resilience training preparation for life after sport. However, these programs are often delivered in a group format, making it difficult to provide individualized support to student-athletes. This project elaborated on the role occupational therapy serves in both program development and the transition out of sport. This …

The Quick Dash Reformed: Increasing The Holisticity And Occupational Nature Of Upper Extremity Therapeutic Evaluation, Lauren E. Garcia

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: The QuickDASH is one of the most common upper extremity (UE) focused patient-reported outcome measures (PROMs) utilized within current practice. Despite its high utilization rate, there are still many concerns surrounding the QuickDASH, including its applicability to certain populations, difficulty with cross-cultural adaptation, and limited psychosocial evaluation. Purpose: The purpose of this study was to develop and implement a reformed version of the QuickDASH, the OT-DASH, that included modernized and occupation-focused language to achieve increased client applicability and yield more accurate comparisons between client perceptions of dysfunction and their true clinical presentations. Theoretical framework: The Person-Environment-Occupation-Performance model was utilized …

Electro-Mechanical Behavior Of Core-Shell Carbon Grease-Silicone Fibers Fabricated Via Coaxial Direct Ink Writing, Fahrettin Kilic

Graduate Theses, Dissertations, and Problem Reports (ETD)

Highly stretchable strain sensors are essential components in soft electronics, enabling integration of sensing and signal transmission into deformable systems. This thesis explores the fabrication and characterization of coaxial core–shell fibers composed of a conductive carbon grease core and an elastomeric silicone shell. These fibers were produced using coaxial direct ink writing (DIW), a versatile additive manufacturing technique that allows continuous deposition of multi-material filaments. The goal was to achieve mechanically compliant yet electrically stable fibers capable of withstanding large deformations, suitable for applications in wearable electronics and soft robotics.
The printed fibers were systematically examined through mechanical tensile testing, …