Ai-Based Smart Proxy Modelling Of Ibdp Field Reservoir Pressure Of Carbon Sequestration, Idongesit Bassey Ansa

Graduate Theses, Dissertations, and Problem Reports (ETD)

The application of artificial intelligence (AI) and machine learning (ML) in engineering holds great promise for addressing the challenge of climate change. One key focus in the fight against climate change is the geological storage of CO2, which has gained significant attention as a crucial strategy for mitigating greenhouse gas emissions. The primary objective is to ensure the safe and controlled containment of injected CO2 over an extended period, which has proven to be a major challenge in the journey toward carbon capture, and geologic storage (CCS). To achieve this goal, several critical tasks must be accomplished. These include ensuring …

Detection And Observed Correlations Of Statin And Beta-Blocking Pharmaceuticals In West Virginia Surface Waters And Ichthyofauna, Joseph W. Kingsbury

Graduate Theses, Dissertations, and Problem Reports (ETD)

Within this thesis the source, presence, and the estimated ecotoxicological effects of cardiovascular medicines, specifically statins and beta-blockers are described within the Buckhannon, Tygart Valley, and West Fork Rivers of West Virginia. Included within this thesis is a general introduction providing key background information and relevant research related to the source, presence, and known toxicity of statins and beta-blockers. Following the general introduction there are 3 distinct chapters. Chapter 1 focuses on the estimated demographic based source loadings and the surface water concentration characteristics of statins and beta-blockers within the three rivers. Chapter 2 investigates the effects of statins and …

Leveraging Artificial Intelligence And Geomechanical Data For Accurate Shear Stress Prediction In Co2 Sequestration Within Saline Aquifers (Smart Proxy Modeling), Munirah Alawadh

Graduate Theses, Dissertations, and Problem Reports (ETD)

This research builds upon the success of a previous project that used a Smart Proxy Model (SPM) to predict pressure and saturation in Carbon Capture and Storage (CCS) operations into saline aquifers. The Smart Proxy Model is a data-driven machine learning model that can replicate the output of a sophisticated numerical simulation model for each time step in a short amount of time, using Artificial Intelligence (AI) and large volumes of subsurface data. This study aims to develop the Smart Proxy Model further by incorporating geomechanical datadriven techniques to predict shear stress by using a neural network, specifically through supervised …

Investigations Into Indole Formation: Base-Mediated Formation Of N-Hydroxy- And N-Alkoxyindoles, Ancillary Ligand Development For Watanabe-Cenini-Söderberg Cyclization, And Total Synthesis Of Racemosin B, Blaine Thomas Mcclay

Graduate Theses, Dissertations, and Problem Reports (ETD)

A novel, base-mediated cyclization was developed to form N-hydroxy- and N-alkoxyindoles from 2-(2-nitrophenyl)butenoates. Potassium tert-butoxide or sodium tert-pentoxide, with or without the in-situ addition of an electrophile, formed a multitude of N-hydroxy- and N-alkoxyindoles. The formation of these butanoate precursors was improved via a Barluenga coupling reaction. Novel 4,5,6,7-tetramethoxy-1,10-phenanthroline was utilized as an ancillary ligand within the Watanabe-Cenini-Söderberg reductive cyclization reaction conditions. These new reaction conditions produced high yields for sterically hindered 2-nitrostyrenes and the first synthesis of dilemmaone A using this reductive cyclization. Racemosin B was synthesized in a three-step process utilizing a …

Learning Representations For Novelty And Anomaly Detection, Ranya Almohsen

Graduate Theses, Dissertations, and Problem Reports (ETD)

The problem of novelty or anomaly detection refers to the ability to automatically

identify data samples that differ from a notion of normality. Techniques

that address this problem are necessary in many applications, like in medical

diagnosis, autonomous driving, fraud detection, or cyber-attack detection, just to

mention a few. The problem is inherently challenging because of the openness of

the space of distributions that characterize novelty or outlier data points. This is

often matched with the inability to adequately represent such distributions due

to the lack of representative data.

In this dissertation we address the challenge above by making several …

A Program Evaluation Of The West Virginia University Health Science Center Interprofessional Education First-Year Curriculum, Kathryn Lynn Smalley

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: The Interprofessional Education (IPE) First-Year Program was started at the West Virginia University Health Sciences Center in 2013 with the goal of providing participants introductory knowledge of the IPE Core Competencies as defined by the Interprofessional Education Collaborative (IPEC). This program transfers 16 different disciplines out of their isolated educational silos and allows them to learn about, for, and with one another. Participants are introduced to foundational knowledge through several didactic sessions over the course of an academic year. Although the program has been increasingly incorporated by many health sciences disciplines since its inception, it has never officially …

Burkholderia Collagen-Like Protein 8, Bucl8, Is An Outer-Membrane Component Of A Novel Efflux Pump And Immunogenic Vaccine Candidate, Megan Elizabeth Grund

Graduate Theses, Dissertations, and Problem Reports (ETD)

Burkholderia spp. are Gram‐negative intrinsically resistant bacteria that are responsible for environmental and nosocomial infections of clinical and biodefense concern. Our laboratory previously identified in silico Burkholderia collagen-like protein 8 (Bucl8) in the BSL-3 pathogens Burkholderia pseudomallei and Burkholderia mallei (Figure 1). The protein sequence of Bucl8 contains tandem outer-membrane efflux protein domains (OEP) typical of efflux systems and a unique collagen-like domain (CL) consisting of a repeating sequence of Glycine-Alanine-Serine (GAS). Because efflux pumps are often a mechanism of pathogenicity and antibiotic resistance, we investigated Bucl8 further. Here, we determined that the locus of bucl8 contains genes …

Enabling Technologies For Chemical Synthesis: I. Selective Microwave Heating; Ii. Synthesis And Regioselective Cyclotrimerizations Of Tethered 1,6-Diynes, Amir Tavakoli

Graduate Theses, Dissertations, and Problem Reports (ETD)

Reaction discoveries, method developments, and technology advancements lie at the heart of synthetic organic chemistry. These innovations are essential for creating and manipulating complex molecules, which are the building blocks of many important chemical compounds, including pharmaceuticals, materials, and agrochemicals. Here, we first describe new methods to prepare neopentylene-tethered (NPT) 1,6-diynes which are valuable substrates for reaction discovery and target-oriented synthesis, especially in benzannulation strategies toward illudalane natural products. NPT 1,6-diynes have been employed as coupling partners in cyclotrimerization reactions for the synthesis of highly substituted benzene rings which present a persistent challenge in chemical synthesis and are underrepresented scaffolds …

Elucidation Of The Role Of Agouti-Signaling Protein Throughout Folliculogenesis And Early Embryonic Development In Cattle, Heather L. Chaney

Graduate Theses, Dissertations, and Problem Reports (ETD)

The oocyte expresses certain genes during folliculogenesis to regulate the acquisition of oocyte competence. Oocyte competence, which refers to the presence of imperative molecular factors in the oocyte that are critical for high oocyte quality, is directly related to the ability of the oocyte to result in a successful pregnancy following fertilization. Over the past few decades, the development and optimization of assisted reproductive technologies, particularly in vitrofertilization, have enabled the beef and dairy industries to advance cattle genetics and productivity. However, only approximately 40% of bovine embryos will develop to the blastocyst stage in vitro. In addition, bovine embryos …

Gender Inclusivity In Italian: Can Gender Neutrality Be Reached In A Gendered Language? Difficulties, Proposals And Public Perception Of The Phenomenon, Gaia Prunotto

Graduate Theses, Dissertations, and Problem Reports (ETD)

Italian is a gendered language in which reference to grammatical gender has been constant and inevitable. As language reflects and shapes society, grammatical gender reinforces binary views of social gender and the invizibilization of non-binary identities. Movements for social justice have inspired gender-fair linguistic innovations, such as the feminization of job titles and gender-neutral markers, to promote gender equity and challenge hierarchies of power and dominance in society. This research aimed to explore gender-neutral forms in the Italian grammatical system and public perceptions of these innovations through two studies with two groups of participants.

The first group of participants (n=27) …

Il-27 Negatively Impacts Immunity Developed Following Bcg Administration, Shelby D. Bradford

Graduate Theses, Dissertations, and Problem Reports (ETD)

Mycobacterium tuberculosis (Mtb) is the causative agent of tuberculosis (TB), a predominantly respiratory pathogen responsible for over ten million infections and one million deaths last year. Despite it being a primary objective of the World Health Organization (WHO) for several decades to irradicate TB, progress toward this objective has fallen short of the 2020 target to end TB by 2035. Meeting this goal will require advancements in diagnostic tools, education, healthcare access, and treatments and preventatives. Regarding prevention, the primary tool for this strategy is through vaccination. Currently, the only licensed vaccine for protection against TB is the century old …

An Assessment Of The Forensic Aspects Of Genetic Genealogy, Sarah Hester

Graduate Theses, Dissertations, and Problem Reports (ETD)

Forensic genetic genealogy has grown in both popularity and controversy in recent years. The genetic information comes from large numbers of single nucleotide polymorphism (SNP) markers. Polymorphism variations can be traced through familial lineage, determining how closely individuals are related to each other based on the similarities and differences in SNPs within their DNA. Polymorphisms account for approximately 85% of human variation. Genealogy and ancestry determination companies trace the lineage of a person back through multiple generations using polymorphisms. As more individuals submit their DNA for analysis the genealogical profile databases continue to grow in size. Law enforcement uses genealogical …

The Investigation Of Novel Bovine Oocyte-Specific Long Non-Coding Rnas And Their Roles In Oocyte Maturation And Early Embryonic Development, Jaelyn Zoe Current

Graduate Theses, Dissertations, and Problem Reports (ETD)

Early embryonic loss is a significant factor in livestock species' infertility, resulting in an economic deficit. In cattle, the in vivo fertilization rate is ~90%, with an average calving rate of about 55%, indicating an embryonic-fetal mortality rate of roughly 35%. Further, 70-80% of total embryonic loss in cattle occurs during the first three weeks after insemination, particularly between days 7-16. Growing evidence indicates that the oocyte plays an active role in regulating critical aspects of the reproductive process required for successful fertilization, embryo development, and pregnancy. However, defining oocyte quality remains enigmatic. Recently, many have abandoned the notion that …

Expansion On The Use Of The Novel Compound, Elp-004, Using A Mycobacterium Tuberculosis Experimentation Model, Kendyl Marie Berry

Graduate Theses, Dissertations, and Problem Reports (ETD)

Osteoarticular tuberculosis (OAT) is a bone degenerative disease that results in bone erosion, joint effusion, joint swelling, and sometimes, nerve compression. OAT occurs from the hematogenous dissemination of Mycobacterium tuberculosis (Mtb) bacilli spreading from the primary site of infection to the bones and joints; the primary site of infection tends to be the lungs as pulmonary tuberculosis infections are the most common. OAT can occur as the result of an active or latent infection. Latent infections result in OAT when bacilli are able to escape granulomas. The incidence of OAT is not as high as pulmonary infections, making up about …

Optimal Path Planning For Aerial Robots Using Genetic Algorithm, Anna Puigvert I Juan

Graduate Theses, Dissertations, and Problem Reports (ETD)

This thesis presents a path optimization solution for a robot in two different constrained 3-dimensional (3D) environments. The robot is required to travel from its current position to a goal position following minimum cost paths (optimal paths). The first environment has 3D obstacles that interfere with the robot’s path. The path cost for this environment accounts for the minimum distance traveled by the robot from the start to the goal position while avoiding obstacles. The second environment is the atmosphere of Venus, specifically a flyable region of this atmosphere with characteristics similar to Earth’s. This environment has strong westward winds …

Synthesis And Pharmacology Of Illudalic Acid And Analogous Chemical Structures, Robert Gaston Jr

Graduate Theses, Dissertations, and Problem Reports (ETD)

Natural products are the foundation of modern medicine and an inspiration for chemical innovation. Developing new synthetic strategies to complex natural products drives synthetic innovation and promotes pharmacological exploration. As bioactive secondary metabolites of fungi, illudalic acid and associated analogs have unrealized medicinal potential due to synthetic limitations. Illudalic acid is notably the first selective covalent inhibitor of the LAR-PTPs, a class of enzymes linked to many human illnesses, including stimulant addiction. The chemistry herein focuses on optimizing synthetic routes to illudalic acid and associated analogs toward exploring their pharmaceutical potential. We report a second-generation synthesis of illudinine (55% overall …

Computational Mechanisms Of Face Perception, Jinge Wang

Graduate Theses, Dissertations, and Problem Reports (ETD)

The intertwined history of artificial intelligence and neuroscience has significantly impacted their development, with AI arising from and evolving alongside neuroscience. The remarkable performance of deep learning has inspired neuroscientists to investigate and utilize artificial neural networks as computational models to address biological issues. Studying the brain and its operational mechanisms can greatly enhance our understanding of neural networks, which has crucial implications for developing efficient AI algorithms. Many of the advanced perceptual and cognitive skills of biological systems are now possible to achieve through artificial intelligence systems, which is transforming our knowledge of brain function. Thus, the need for …

Drought Effects On Biofuel Feedstock Production By Populus Trichocarpa, Marvin Jacob Wright

Graduate Theses, Dissertations, and Problem Reports (ETD)

As the world population continues to increase, so does the need for sustainable sources of fuel. Biofuels are of particular interest and could be an economically feasible fuel source given the right conditions. Populus trichocarpa, is a rapidly growing plantation species that, in addition to having a fully sequenced genome available for study, displays a wide range of phenotypic traits among genotypes. By analyzing these differences in both plantation and more controlled greenhouse settings, we aimed to discover which genotypes performed the best under drought conditions, and which physiological mechanisms granted them that high performance. In the field, differences in …

Structural Health Monitoring Using Machine Learning And Synthetic Data, Michail Tzimas

Graduate Theses, Dissertations, and Problem Reports (ETD)

Structural health monitoring spans many decades of research across multiple engineering fields. However, typical monitoring processes for damage detection of complex structures usually prohibit real-time or fast detection of debilitating damage to the structure. One of the major issues of real-time detection of damage is the enormity of data that needs to be processed, which is worsened by the relative inability of fast relaying of data to structural engineers. With the rapid advancement of Machine Learning, both issues can be overcome, and detection of failure is achieved with non-invasive techniques. This dissertation explores the applicability of Machine Learning as a …

The Effects Of E-Cigarette Aerosol On The Force Degradation Of Elastomeric Chain: An In Vitro Pilot Study, Nicholas David Palmer

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background and Objectives

An ideal orthodontic force system should exert continuous light force. An orthodontist has the option to utilize different systems for closing orthodontic extraction space. A commonly used method when closing extraction space is to utilize orthodontic elastomeric chain. Energy Chain® (Rocky Mountain Orthodontics, Franklin, IN) allows for less deformation of shape following the initial stretch, more consistent force, and less force degradation over time. The mouth is a harsh environment that experiences daily changes in pH and temperature. These changes cause elastomeric chain to experience increased force degradation. The creation of the JUUL® (JUUL Labs, San Francisco, …

A Comparison Of The Failure Rate Of Two Orthodontic Light-Curable Adhesives: A Chart Review, Ian Montgomery Weaver

Graduate Theses, Dissertations, and Problem Reports (ETD)

A Comparison of the Failure Rate of Two Orthodontic Light-Curable Adhesives: A Chart Review

Ian Montgomery Weaver, D.D.S.

Background and Objectives: The overhead costs of running a private orthodontic practice are infamously high, thus there is a notable benefit to finding the most cost effective methods of treating patients while still maintaining high quality care. A relatively new light-cure orthodontic adhesive BracePaste®^® (American Orthodontics™, Sheboygan, WI), introduced to the market in 2017, can fluoresce under UV light to assist in identifying the difference between the enamel of the tooth and the adhesive material itself, an ability which can aid …

The Effect Of Face Interpretation Training On Social Anxiety Symptoms, Grace L. Wheeler

Graduate Theses, Dissertations, and Problem Reports (ETD)

According to cognitive models of social anxiety disorder (SAD), individuals place excessive importance on gauging others’ reactions (e.g., facial expressions) to their social performance. Facial interpretation bias modification (FIBM) was developed to shift interpretations of ambiguous faces. The current study uses FIBM to test whether face interpretation is causally related to SAD symptoms in an unselected adult sample. Participants (N=139) were randomized to 1 of 3 conditions (positive, negative, accurate) in a single-session, online study. Pre- and post- intervention measures of face interpretation and SAD were completed. In line with hypotheses, individuals in the positive condition interpreted faces more positively …

Deadly Divisions: Class And Stigma As Fundamental Social Causes Of Spatial Health Inequalities, Misty Lee Harris

Graduate Theses, Dissertations, and Problem Reports (ETD)

The objective of this dissertation is to investigate how class and stigma influence spatial inequalities in health across the US, from the structural to the individual level. Class, stigma, and subsequent access to capital resources are not equally distributed across the US. Women, poor, and minority populations continue to have unequal access to capital resources across the country, though this is spatially determined. Similarly, while there are health inequalities along the same social cleavages at the national level, they differ significantly across localities. Research has not paid enough attention to the fundamental social causes of inequities, resulting in the inability …

The Effect Of Silver Carp On Large River Community Size Spectra, Bradley R. Novak

Graduate Theses, Dissertations, and Problem Reports (ETD)

The introduction of silver carp (Hypophthalmichthys molitrix) has had dramatic effects on native fish communities. Silver carp are large bodied planktivores and compete with native planktivores which can cause cascading effects throughout the food web. Our study strives to determine the threshold of abundance beyond which silver carp alter fish community structure. We used a community size spectra approach to evaluate fish community structure and compare size spectra across spatial and temporal gradients of silver carp abundances. Community size spectra (CSS) is an ataxic approach quantifying the predictable decrease in fish abundance with increasing body size that reacts …

Longitudinal Oxygen Imaging In 3d (Bio)Printed Models, Ryan Curtis O'Connell

Graduate Theses, Dissertations, and Problem Reports (ETD)

Electron paramagnetic resonance (EPR), and its molecular imaging modality, is a powerful tool to noninvasively map various biological and chemical markers within objects of interest. Reliable data acquisition is a major impeding factor for longitudinal hands-off measurements. Measurements are especially challenging in biomedical applications, as live objects are not static. Frequent changes occur that require constant fine recalibration of the EPR detection system, called the resonator. To enable longitudinal imaging, a technology permitting automatic digital control of resonator coupling, tuning, and EPR data acquisition was developed. Automation was achieved through the utilization of a microcontroller and digital peripheral components such …

Blowdown Wastewater Co-Treatment With Produced Water For Resource Recovery: Moving Towards Zero Liquid Discharge And Sustainable Waste Management, Mohammad Hafez Ahmed

Graduate Theses, Dissertations, and Problem Reports (ETD)

Cooling of low-pressure steam and flue gas desulfurization (FGD) process at coal-fired powerplants often require large quantities of freshwater and can generate waste streams from the blowdown (BD) operations. Treating these wastewaters for reuse can offset the freshwater demand, which is beneficial especially in semiarid and arid regions. However, the traditional treatment (i.e., chemical softening and evaporation) of cooling BD water (BDW) is chemical- and energy-intensive and adds to the overall costs of water management for powerplants. Furthermore, the updated Effluent Limitation Guidelines and Standards for powerplants set forth by the U.S. Environmental Protection Agency (EPA) …

Right Treatment Wrong Time: Immunotherapy Administration Post-Radiotherapy Decreases Tumor Burden In A Preclinical Model Of Brain Metastasis, Kathryn Elizabeth Blethen

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation (a) provided an in-depth literature review of methods to modulate the blood-brain and blood-tumor barriers to increase drug delivery and efficacy in brain metastases, (b) evaluated the effects of whole-brain radiation therapy on the blood-brain barrier in immunocompetent and immunocompromised mouse models and proposed a mechanism by which the immune response to radiation disrupts the blood-brain barrier, and (c) developed a syngeneic lung cancer brain metastasis model to determine the impact of coordinated immunotherapy administration with radiotherapy. The blood-brain barrier is an impediment to drug delivery to the brain. The inherent leakiness of the blood-tumor barrier does not …

Elucidating Mechanisms Of Proteasome Modulation By Small Molecules To Treat Neurodegenerative Diseases And Cancer, Janelle Joo Yee Chuah

Graduate Theses, Dissertations, and Problem Reports (ETD)

Maintaining protein homeostasis, or proteostasis, is crucial for cellular function, and protein degradation through the proteasome plays a pivotal role in this process. Dysregulation of the proteasome can lead to proteostasis collapse and has been linked to the development of neurodegenerative diseases and cancers. Neurodegenerative diseases often exhibit proteasome impairment, whereas proteasome upregulation is frequently observed in cancer. Although the use of proteasome inhibitors as a pharmaceutical approach has shown success in certain cancers, it has yet to demonstrate efficacy in neurodegenerative diseases due to the absence of drug-like small molecules capable of effectively reversing proteasome impairment and/or activating the …

Machine Learning For Biosensors, Gayathri Anapanani

Graduate Theses, Dissertations, and Problem Reports (ETD)

Biosensors have become increasingly popular as diagnostic tools due to their ability to detect and quantify biological analytes in a wide range of applications. With the growing demand for faster and more reliable biosensing devices, machine learning has become a valuable tool in enhancing biosensor performance. In this report, we review recent progress in the application of machine learning to biosensors. We discuss the potential benefits of using machine learning in biosensors, including improved sensitivity, selectivity, and accuracy. We also discuss the various machine learning techniques that have been applied to biosensors, including data preprocessing, feature extraction, and classification and …

Energy Conversion In Plasmas Out Of Local Thermodynamic Equilibrium: A Kinetic Theory Perspective, Mahmud Hasan Barbhuiya

Graduate Theses, Dissertations, and Problem Reports (ETD)

The study of energy conversion in collisionless plasmas that are not in local thermodynamic equilibrium (LTE) is at the leading edge of plasma physics research. Plasma constituents in such systems can exhibit highly structured phase space densities that deviate significantly from that of a Maxwellian. A standard approach has emerged in recent years for investigating energy conversion between bulk flow and thermal energy in collisionless plasmas using the non-LTE generalization of the first law of thermodynamics. The primary focus is placed on pressure-strain interaction (PS) term, with a particular emphasis on its non-LTE piece called Pi − D. Recent studies …