Digital Commons Network^™

Evaluating The Recovery Of Dna From Adhesive Tape After Exposure To Heat And Humidity: Assessing The Degradation Index And Str Profile, Emily Davis

Graduate Theses, Dissertations, and Problem Reports (ETD)

It is not uncommon for forensic DNA samples to be degraded or of low quantity; these types of samples can pose challenges throughout analysis and an informative STR profile is not guaranteed. DNA analysis is also very expensive and timely; therefore, it is necessary to understand where to sample a piece of evidence to obtain the best quality and quantity of DNA from the substrate, especially in the case of trace DNA samples which may be difficult to detect. It is also necessary to know when to proceed with analysis given the quality of the sample. The aim of this …

Watching You Watching Me: Lateral Surveillance In Singapore, Yan Song Lee

Graduate Theses, Dissertations, and Problem Reports (ETD)

Historically, top-down surveillance -- surveillance from the state and other organizations -- has dominated social life, but not anymore. Technological advances drove down the price of surveillance equipment which aided in the proliferation of devices such as dashboard cameras. The widespread use of affordable surveillance equipment gave rise to the phenomenon of lateral surveillance, whereby laypersons can employ surveillance techniques to monitor individuals around them. The Singapore metropolis provides an excellent example with the ubiquitous use of dashboard cameras for cars today. In this research, I focus on Singaporean rideshare drivers who are simultaneously subjected to traditional forms of top-down …

An Exploration Of Diversity And Inclusion In Introductory Physics, Rachel J. Henderson

Graduate Theses, Dissertations, and Problem Reports (ETD)

Diversity and inclusion has been a concern for the physics community for nearly 50 years. Despite significant efforts including the American Physical Society (APS) Conferences for Undergraduate Women in Physics (CUWiP) and the APS Bridge Program, women, African Americans, and Hispanics continue to be substantially underrepresented in the physics profession. Similar efforts within the field of engineering, whose students make up the majority of students in the introductory calculus-based physics courses, have also met with limited success. With the introduction of research-based instruments such as the Force Concept Inventory (FCI), the Force and Motion Conceptual Evaluation (FMCE), and the Conceptual …

Effect Of Natural Gas Fuel Composition On Gaseous And Particle Numbermass Emissions From A Heavy-Duty Natural Gas Engine Used In Unconventional Well Development Operations, Sashank Jammalamadaka

Graduate Theses, Dissertations, and Problem Reports (ETD)

Natural gas (NG) reserves have been discovered in abundance with the aid of various technological advancements in the field of directional drilling and hydraulic fracturing. Prime movers of unconventional well development were previously identified to be over-the-road (OTR) trucks, drilling engines, and hydraulic stimulation engines. The increased NG reserves have driven the industry to shift from using diesel fueled engines to natural gas engines. This allows the industries to take advantage of the produced natural gas to be directly used as energy source to fuel the prime movers involved with unconventional well development.;The primary objective of this study is to …

Environmental Controls, Similitude, And Scaling Of Stream Dissolved Oxygen Across The Pacific Coast Of U.S.A, Filmon Araya

Graduate Theses, Dissertations, and Problem Reports (ETD)

Dissolved oxygen (DO) is a key indicator for water quality and the general health of stream ecosystems. However, the dynamics of stream DO is controlled by myriad interacting environmental drivers. This thesis investigates the major environmental drivers, emergent similitude (similarity leading to parametric reductions), and scaling of stream DO across the Pacific Coast of U.S.A. The research was conducted by assembling a dataset for 1998--2015 at 35 water quality monitoring stations of U.S. Geological Survey and Environmental Protection Agency. Pearson correlation matrix, principal component analysis, and factor analysis were employed to identify the interrelations and groupings of stream DO and …

Tobacco Use, Number Of Serious Smoking Cessation Attempts, And Interest In Lung Screening In A Sample Of Adult Muslims In The United States, Omar F.S. Attarabeen

Graduate Theses, Dissertations, and Problem Reports (ETD)

Muslims in the United States (US) exhibit higher rates of tobacco use in comparison with rest of the US population. As a result, US Muslims might be at a higher risk for negative health consequences of tobacco use such as lung cancer. Investigating factors that are associated with tobacco use, number of smoking cessation attempts, and interest in lung screening in adult US Muslims can facilitate future efforts aimed at improving health outcomes, essentially through reducing tobacco use rates and promoting preventive lung screening in this population. Therefore, the current dissertation aimed to investigate the association of Social Cognitive Theory …

Transforming Undergraduate Stem Education: Reforming Classrooms By Training Current And Future Faculty, Karen E. Bailey

Graduate Theses, Dissertations, and Problem Reports (ETD)

In the absence of formal pedagogical training, educators tend to teach the way they were taught, using traditional lecturing. Numerous studies have shown that engaging students in active learning improves student learning and retention of knowledge. Reforming STEM education has become a national imperative. Since the turn of the millennium, many initiatives have sprung forth in response to a growing awareness of the need for reform in post-secondary biology education. The National Academies Summer Institutes (NASI) is one such initiative. The Summer Institutes provide training in evidence-based strategies for biology educators from primarily research universities where lecture-based teaching in large …

An Exploration Of Paul Bowles' Piano-Solo Pieces, Juan Carlos Rios Betancur

Graduate Theses, Dissertations, and Problem Reports (ETD)

This research paper provides a general overview of the piano-solo literature by the American composer Paul Frederic Bowles (1910-1999). Thanks to recent contributions, this repertoire is now available in recordings and musical scores as it has never been before.;This paper is divided into two sections. The first covers the biography of Paul Bowles and his musical achievements as a composer, along with his research into the folk music of Morocco and his literary writings as a music critic for the journal Modern Music and for The New Herald Tribune. The second part is about Bowles' piano-solo output, divided thematically into …

What The Frost Killed, Allison Semele Blair

Graduate Theses, Dissertations, and Problem Reports (ETD)

What the Frost Killed is the culmination of two years spent memorializing the dead plants and animals of my immediate surroundings. Primarily focusing on birds who die as a result of human activity, I seek to pay attention to and elevate the individual victims of a systemic problem. Inspired by Ancient Greek tragedy, I engage with the ethical dilemmas inherent in our collective actions to express the strange, beauty of these unsung deaths.;My thesis body of work draws on a decade of research exploring the intersection between political history and ancient tragic literature. As I studied these stories written in …

Experimental Analysis Of Unsteady Aerodynamic Covert Feather Behavior, Laura J. Ordaz

Graduate Theses, Dissertations, and Problem Reports (ETD)

The interest in man-made flying vehicles has existed for over 100 years. Bird flight has been the major inspiration for the creation of such vehicles. More recently, UAVs (unmanned aerial vehicles) have become more popular and their use in more applications has developed the need for improved maneuverability. UAVs are also decreasing in size which has allowed them to operate within similar flight conditions as birds. Studying bird feather behavior may open doors and shed some light as to how the bird achieves such agility and control, which may then be adapted to UAV flight. In this research, the motion …

Growing Up Between Two Worlds: A Portrait Of Second-Generation American A Multimedia Project, Dalia Ahmed Elsaid

Graduate Theses, Dissertations, and Problem Reports (ETD)

This professional project is a multimedia project focusing on stories of three second-generation Americans in West Virginia. The project is disseminated using a website. It includes content curated by the project author (photos, videos, written stories, data visualization) and information selected by the participants themselves. The content will add to the information available on different cultural communities in West Virginia in general, and on second-generation Americans in particular. It is hoped the project, through its portrayals of these individuals and their immigrant families, will help to dispel and counter some of the stereotypical depictions of immigrants. With this goal in …

Growth Media Alternatives For Mine Refuse Cap And Cover Systems, Sitraka Jean D'Annie Rabemanjakasoa

Graduate Theses, Dissertations, and Problem Reports (ETD)

In 1999, Royal Scot Minerals based in Greenbrier County, West Virginia, USA went bankrupt and abandoned a 47-acre coal refuse pile, which currently presents environmental and economic issues. The primary objective of this problem report is to identify an effective approach to create a growth layer that establishes a sustainable vegetation on this pile. Using available data in the relevant literature, this report evaluates topsoil substitutes using three materials: papermill sludge, sewage sludge and lumber mill waste application for coal refuse piles. Like papermill sludge, sewage sludge reduces erosion/sedimentation by improving the soil water holding capacity and decreases the surface …

Survey For Naturally Occurring Radioactive Materials At A Gas Fractionation Plant, Brittany Diane Jones

Graduate Theses, Dissertations, and Problem Reports (ETD)

Marcellus Gas Company (MGC) is a midstream energy company operating natural gas gathering and processing assets in the Marcellus Shale region, primarily in Ohio, West Virginia, and Pennsylvania. At the time of the study, MGC was interested in surveying for naturally occurring radioactive material (NORM) at their primary fractionation facility in the region, the Ohio River Fractionation Plant. A NORM survey, consisting of over 300 measurements, was conducted at the site to evaluate the need to implement the company's written NORM program and to assess for additional controls to protect employees against ionizing radiation exposure. Additionally, a qualitative exposure assessment …

Forecasting Future Energy Production Using Hybrid Artificial Neural Network And Arima Model, Maryam Khodaverdi

Graduate Theses, Dissertations, and Problem Reports (ETD)

The objective of this research is to obtain an accurate forecasting model for the amount of electricity (in kWh) that is generated from different primary energy sources in the U.S. In this research, Artificial Neural Network (ANN) and hybrid ARIMA and ANN algorithms were developed that can be used for forecasting the amount of energy production in the short, as well as, in the long run. Based on the inferences made from the available data provided by Energy Information Administration from January 2004 to December 2014, two different forecasting models for each primary energy source were constructed. These two models …

Investigation Into Composting Efforts At Wvu: A Case Study., Marina Alexis Berry

Graduate Theses, Dissertations, and Problem Reports (ETD)

Institutional composting systems have been adopted by many land grant universities to help better manage their waste and protect the environment. West Virginia University (WVU) has yet to begin formally managing their animal manure and waste at the university farms. This investigation will look into what has been done at other land grant universities to see which methods could be applied at WVU. This could have environmental, economic, and social benefits and also serve as a living learning classroom. University composting systems have often started small and grown to accommodate campus food waste, which could also be a possibility for …

Instrumentation And Inertial Navigation Systems Design For Tensegrity Robot Implementations, Scott Edward Harper

Graduate Theses, Dissertations, and Problem Reports (ETD)

One of the major challenges faced when developing missions for the exploration of planetary bodies is the risk these terrains pose on the science platform when using a traditional lander or wheeled rover. One means of developing platforms to traverse these harsh terrains is to utilize mobility systems comprised of tensegrity structures. These structures have the capacity to distribute loads across a network of axially loaded members such that they can be constructed in a very lightweight manner and morph their geometries when required. In literature, there has been significant progress in simulated environments to utilize tensegrity structures as mobility …

Cortactin Phosphorylation By Casein Kinase 2 Regulates Actin Related Protein 2/3 Complex Activity And Invadopodia Function, Steven Michael Markwell

Graduate Theses, Dissertations, and Problem Reports (ETD)

Malregulation of the actin cytoskeleton enhances tumor cell motility and invasion. The actin-binding protein cortactin facilitates branched actin network formation through activation of the actin-related protein (Arp) 2/3 complex. Arp2/3 complex activation is responsible for driving increased migration and extracellular matrix (ECM) degradation by governing invadopodia formation and activity. While cortactin-mediated activation of Arp2/3 complex and invadopodia regulation has been well established, signaling pathways responsible for governing cortactin binding to Arp2/3 are unknown. In this dissertation we identify casein kinase (CK) 2α phosphorylation of cortactin as a negative regulator of Arp2/3 binding. CK2α directly phosphorylates cortactin at a conserved threonine …

Explanatory And Causality Analysis In Software Engineering, Yasser Ali Alshehri

Graduate Theses, Dissertations, and Problem Reports (ETD)

Software fault proneness and software development efforts are two key areas of software engineering. Improving them will significantly reduce the cost and promote good planning and practice in developing and managing software projects. Traditionally, studies of software fault proneness and software development efforts were focused on analysis and prediction, which can help to answer questions like `when’ and `where’. The focus of this dissertation is on explanatory and causality studies that address questions like `why’ and `how’.

First, we applied a case-control study to explain software fault proneness. We found that Bugfixes (Prerelease bugs), Developers, Code Churn, and Age of …

Understanding The Novice Decision-Making Process In Forensic Footwear Examinations: Accuracy And Decision Rules, Madonna A. Nobel

Graduate Theses, Dissertations, and Problem Reports (ETD)

The reproducibility of experienced-based forensic pattern interpretation is founded on the notion that domain-specific knowledge can be successfully distributed and applied among experts within a group. This assumption persists, even when the examination is complicated by variations in case circumstances, such as impression clarity and totality, as well as media, substrate, collection mechanism and enhancement. While it is further theorized that many of these factors (as well as additional confounding factors) are at play during an examination, the manner and extent to which these sources of variability affect the examination of footwear evidence remain unclear. In order to explore this …

Morphological, Genetic And Biochemical Characterization Of The Anti-Malarial Herb, Artemisia Annua Germplasm Collection At West Virginia University, Delini K. Samarasinghe

Graduate Theses, Dissertations, and Problem Reports (ETD)

Malaria is one of the deadliest diseases in human history. Nearly half of the world’s population, is at the risk in 106 countries. Only in 2016, this disease killed about 445,000 people, 72% of them being children under age five. It also accounts for US $12 billion dollars of direct costs in Africa alone. Five different species of Plasmodium cause malaria but P. falciparum is the most detrimental one, causing 50% of all malaria cases and is considered as the deadliest parasite in humans. Artemisinin (ART), a 15 C sesquiterpenoid is currently the only precursor to the most effective anti-malarial …

Crop Rotations That Integrate Pasture Are Less Vulnerable To Nitrogen Leaching Due To Changes In Microbial Community Composition And Reduced Nitrification, Jeth Gv Walkup

Graduate Theses, Dissertations, and Problem Reports (ETD)

Agroecosystems occupy a large portion of the United States and receive a disproportionally high amount of terrestrial nitrogen inputs. As application rates increase, nitrogen use efficiency declines leading to higher rates of nitrogen loss. To develop sustainable agricultural practices, the long-term effects of management practices on the soil microorganisms that perform central roles in the transformation of nitrogen need to be studied. To study the long-term impacts of compost application and pasture integration in crop rotation, samples were collected from the existing 19-year-old Organic Crop Livestock Field (OCLF) experiment on the WVU organic research farm. The OCLF experiment includes a …

Association Of Diet Quality And Body Mass Index With Serum Ceramides In Middle-Aged Adults, Margaret Ann Drazba

Graduate Theses, Dissertations, and Problem Reports (ETD)

Cardiovascular disease (CVD) is one of the leading causes of death worldwide and identifying CVD risk is important for determining and implementing appropriate intervention strategies. Ceramides are a class of sphingolipids that are implicated in the pathogenesis of obesity and CVD. However, their relationship to diet quality, a modifiable risk factor for CVD, is largely unknown. The purpose of this cross-sectional study was to determine the association of an a priori diet quality index and body mass index (BMI) with circulating ceramides in middle-aged adults (n=96). Dietary intake was assessed using three 24-hour recalls and Nutrition Data System for Research …

Applications Of Cellular Components In Engineered Environments, Xiao Hu

Graduate Theses, Dissertations, and Problem Reports (ETD)

Manipulation of cellular components in synthetic environment has attracted interest for applications ranging from sensors to nanelectronics and from catalysis to biomedical devices. In such applications, biological-based processes such as specific recognition and self-assembly direct the formation of hierarchical structures, all at minimum energetic costs, with high efficiency and reliability.

Among such cellular machines, microtubules and kinesins have attracted special interest. Inside the cells, microtubules are giving structural integrity while serving as regular and uniform tracks for transport of vesicles or organelles. Kinesin uses a microtubule track in vivo to progress to specific locations with processive and coordinated steps, all …

Arl2bp, A Novel Ciliopathy Protein, Is Required For Cilia Microtubule Formation, Abigail Ruth Moye

Graduate Theses, Dissertations, and Problem Reports (ETD)

Cilia are specialized organelles essential for cellular function. Not surprisingly, mutations in cilia- related genes are linked to multi-syndromic diseases termed ciliopathies. These include blinding diseases such as retinitis pigmentosa (RP). One such novel gene is ARL2BP (ARL2-binding protein) and is linked to RP and situs inversus (organ reversal) in humans, a phenotype produced by defects in the nodal cilia of developing embryos. Defects in photoreceptor cilia, as well as situs inversus in human patients, suggest that ARL2BP plays an invaluable role in the structure and function of cilia. However little is known about the role for this protein in …

Differentiating Human Populations Based On K-Mer Classification Of Hand Bacteria, Thrisha Doppala

Graduate Theses, Dissertations, and Problem Reports (ETD)

Bacterial communities found in and on the human body are not only used in studying human health conditions but are also effective in differentiating individuals due to their distinct profiles. Human palm regions harbor relatively more diverse bacterial communities and are indicative of population groups, life styles, geographic locations, age groups and health conditions. Sequences extracted from hypervariable region V3 of the 16S rRNA bacterial gene of hand bacterial samples from 9 different population groups were classified into Operational Taxonomic Units (OTU) with GreenGenes reference taxonomy using RDP (Ribosomal Database Project) classifier. Frequencies of identified OTUs were used to study …

Characteristics Of Activated Carbons Produced From Herbaceous Biomass Feedstock, Oluwatosin Jerry Oginni

Graduate Theses, Dissertations, and Problem Reports (ETD)

Energy security and environmental protection are at the forefront of research due to the forecasted depletion of fossil fuel reserves and the growing concern of its adverse environmental impacts. The use of renewable sources for energy applications has centered on the use of biomass feedstocks and this include from herbaceous, woody, agricultural waste, forest residues and municipal wastes. The thermochemical conversion (pyrolysis) of these biomass feedstocks has potential to produce liquid fuel, a solid residue (biochar) and non-condensable gas. The biochar is primarily composed of renewable porous carbon and other inorganic compounds. The biochar is used for soil amendment, adsorption …

Roofing Practices And Fall Prevention In The Construction Industry, Hadi A. Shishtar

Graduate Theses, Dissertations, and Problem Reports (ETD)

Roofing is an important profession in the construction industry yet there is a lack of understanding of its safeness. It is generally believed that roofing is an occupation that can be easily practiced by anyone. However, there are many key factors that are associated with roofing, which may impact the lives of roofers who often face major risks at work. According to the Occupational Safety and Health Administration (OSHA), roofing is one of the leading causes of falling deaths among construction workers. The Bureau of labor statistics has reported that roofers are highly exposed to non-fatal injuries. They have also …

The Microvascular And Systemic Effects Of Titanium Dioxide Nanoparticle Exposure: A Physiological Perspective, Alaeddin Bashir Abukabda

Graduate Theses, Dissertations, and Problem Reports (ETD)

Titanium dioxide nanoparticles (nano-TiO₂) are one of the most widely used engineered nanomaterials (ENM). Their ubiquitous inclusion warrants a more thorough understanding of their toxicity, particularly in sensitive populations such as pregnant women and the developing fetus.

The aim of the first study was to determine the most sensitive segments of the vasculature to pulmonary ENM exposure. Macrovascular function was investigated in the thoracic aorta, common femoral artery and 3^rd order mesenteric arterioles using wire myography. Microvascular function was assessed in 4^th and 5^th order mesenteric arterioles via pressure myography. Nano-TiO₂ exposure induced impairments …

Medical Celebrity In Eighteenth-Century Britain, Katherine Richards

Graduate Theses, Dissertations, and Problem Reports (ETD)

Medical Celebrity in Eighteenth-Century Britain argues that the cultural mechanisms responsible for creating and sustaining celebrity culture helped create and sustain commercialized medicine in eighteenth-century Britain. I identify the process by which celebrity and medical culture impact one another as a sociocultural phenomenon that I term medical celebrity. The following chapters present four case studies of how medical celebrity impacted the development of the medical profession, the medical market, cultural representations and perceptions of health and illness, and the patient experience. I engage with the work done by scholars in contemporary and early celebrity studies to shed light on the …

Using Artificial Intelligence And Machine Learning To Develop Synthetic Well Logs, Marwan Mohammed Alnuaimi

Graduate Theses, Dissertations, and Problem Reports (ETD)

There has been an increase in the need for energy in the recent past. Oil and gas stand as the source of energy that are widely used. The oil and gas reservoirs are targeted for the purposes of field development. The conventional methods of reservoir characteristics require computing techniques that are unique and complex, some of which are labor and time intensive. Mohaghegh argues that all efforts must be tried and made possible to apply Petroleum Data analytics in production and management of reservoir so as to earn a maximum return (Mohaghegh, Shale Analytics, 2017). Different methodologies have been applied …