Quantitation Of Fentanyl And Metabolites From Blow Fly Tissue And Development Effects Of Fentanyl On Lucilia Sericata, Joseph Allen Cox

Graduate Theses, Dissertations, and Problem Reports (ETD)

Death investigations often involve collaborative work between different forensic disciplines, especially with regards to pathology, toxicology, and entomology. This dissertation investigates the effects of designer opioids on the development of blow fly (Lucilia sericata) larvae and the ability to detect and quantify the drugs and/or metabolites present in each stage of the fly’s life cycle. Therefore, we evaluated human tissue fortified with fentanyl at four treatment concentrations, and blow fly larvae were placed to feed on the drug infused tissue. A quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction method was developed, validated, and applied to extract …

Single And Differential Morph Attack Detection, Baaria Chaudhary

Graduate Theses, Dissertations, and Problem Reports (ETD)

Face recognition systems operate on the assumption that a person's face serves as the unique link to their identity. In this thesis, we explore the problem of morph attacks, which have become a viable threat to face verification scenarios precisely because of their inherent ability to break this unique link. A morph attack occurs when two people who share similar facial features morph their faces together such that the resulting face image is recognized as either of two contributing individuals. Morphs inherit enough visual features from both individuals that both humans and automatic algorithms confuse them. The contributions of this …

Modified Firearm Discharge Residue Analysis Utilizing Advanced Analytical Techniques, Complexing Agents, And Quantum Chemical Calculations, William J. Feeney

Graduate Theses, Dissertations, and Problem Reports (ETD)

The use of gunshot residue (GSR) or firearm discharge residue (FDR) evidence faces some challenges because of instrumental and analytical limitations and the difficulties in evaluating and communicating evidentiary value. For instance, the categorization of GSR based only on elemental analysis of single, spherical particles is becoming insufficient because newer ammunition formulations produce residues with varying particle morphology and composition. Also, one common criticism about GSR practitioners is that their reports focus on the presence or absence of GSR in an item without providing an assessment of the weight of the evidence. Such reports leave the end-used with unanswered questions, …

Optimization Of Operating Conditions And Design Parameters On Coal Ultra-Fine Grinding Through Kinetic Stirred Mill Tests And Numerical Modeling, Francisco Patino

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study investigated the ultra-fine coal grinding performance of four low- to moderate-cost grinding media: ceramic balls, granular sand, alumina beads, and silica beads. The kinetic grinding tests were conducted using a stirred mill, while different operating conditions, including the media size, solids concentration, and the addition of viscosity modifiers, were examined. Characterization studies in terms of particle size, shape, and surface morphology were further performed to support the experimental findings. Moreover, using population balance modeling, grinding simulation was performed in Matlab to forecast the particle size distribution based on the specific energy input and experimental size-related data. The results …

More Than One Story: A Bioecological Model Of Elementary Educators’ Perceptions And Representations Of Diversity, Angela Curfman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Classroom teachers have long employed children’s literature in the classroom. Exposure to children’s literature offers a plethora of gains and benefits. Through the usage of children’s literature, critical thinking skills are fostered and factual information is obtained (Hancock, 2000). Varied cultures, linguistic backgrounds, families with diverse socio-economical, and academic backgrounds constitute our public school classrooms. Children’s literature provides readers an opportunity for self-affirmation; therefore, they often seek a mirror in books, (Bishop, 1990). The thoughtful selection of children’s literature in the classroom is essential. Through the lens of Bishop’s (1993) metaphorical notion of books as mirrors, windows, and sliding glass …

Neutral Isn’T Neutral: An Analysis Of Misinformation And Sentiment In The Wake Of The Capitol Riots, Daniel Robert Walsh

Graduate Theses, Dissertations, and Problem Reports (ETD)

January 6th, 2021 was a significant moment in the history of the United States of America. Protestors stormed the Capitol building over the results of the 2020 presidential election in which Joseph R. Biden defeated incumbent president Donald J. Trump. The Capitol riots were partially incited by the presence of misinformation on social media and was an example of the power misinformation has. This study presented two questions. Question one pertains to the sentiment analysis of verified Twitter users and their sentiment towards Trump. Question two pertains to analyzing tweets from verified accounts for misinformation between the dates of January …

Three Essays In Applied Econometrics: Agricultural And Energy Economics, Kuan-Ming Huang

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation examines three empirical issues in energy and agricultural economics using econometrics models whose titles are: 1) Do Natural Hazards in the Gulf Coast Still Matter for State-Level Natural Gas Prices in the US? Evidence After the Shale Gas Boom; 2) Do Exploitations of Marcellus and Utica Shale Formations Improve Regional Economy in Ohio, Pennsylvania, and West Virginia? A Synthetic Control Analysis; and 3) How Did Covid-19 Impact US Household Food Spending? An Analysis Six Months In.

The first essay assesses the impact of natural hazards on state-level natural gas prices and evaluates the effects of the shale gas …

Understanding The Variable Drivers Of Toxicity For The Broad Class Of Carbon Nanotubes And Nanofibers From U.S. Facilities, Kelly E. Fraser

Graduate Theses, Dissertations, and Problem Reports (ETD)

Pulmonary exposure to carbon nanotubes or nanofibers (CNT/F) is known to induce inflammation, toxicity, or tumorigenesis, and is a concern in the occupational setting. U. S. facility employees are at risk of inhalation exposure of multi-walled carbon nanotubes and carbon nanofibers during primary and secondary manufacturing. To date, only one MWCNT, Mitsui-7 has been classified as possibly carcinogenic to humans (Group 2B), while all other materials were subsequently categorized as unclassifiable (Group 3). This class of material has recently been listed as a high priority to the International Agency for Research on Cancer due to this significant knowledge gap. Furthermore, …

In Vitro And In Vivo Toxicological Evaluation Of Emissions From The Fused Filament Fabrication Three-Dimensional Printing, Mariana T. Farcas

Graduate Theses, Dissertations, and Problem Reports (ETD)

Fused filament fabrication (FFF), a three-dimensional (3-D) printing process, is an emerging technology that has recently gained wide popularity among both consumers and manufacturers. As filament is heated to above its glass transition temperature in a 3-D printer, a portion may undergo thermal decompostion, which releases ultrafine particles (UFP) and volatile organic compounds (VOCs) with potential adverse respiratory health effects are released into the air. This study's central hypotheses is that emissions generated during 3-D printing are toxic and exposure to these emissions induces pulmonary and systemic adverse health effects. Considering that currently, limited understanding is available on the health …

Level Of Adoption Of West Virginia Agriculture Teachers' Usage Of Internet-Based Educational Technology In The Classroom, Travis Daniel Veach

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this study was to determine the level of adoption of West Virginia agricultural educators’ usage of internet-based educational technology for instruction. The population for this study included 103 educators during the 2020-2021 school year. The study yielded thirty-two responses from the population. Respondents indicated they use internet-based educational technology for their classroom instruction. Respondents indicated they use internet-based educational technology in a variety of different ways for instruction. Respondents adopted internet-based educational technology at varying degrees depending on the technology, some respondents being early adopters, and others later adopters.

Development And Dynamics Of Us Welfare Policy, Liza Gordon

Graduate Theses, Dissertations, and Problem Reports (ETD)

In this dissertation I analyze the development of contemporary US welfare policy with special consideration given to the importance of race and gender. In the introductory chapter I outline how the development of the American welfare state has continuously neglected the needs of women and minorities as well as how classist, racist, and sexist appeals have been prevalent throughout US history in relation to welfare policies. The remaining chapters analyze how contemporary welfare policies including the 1996 welfare reforms and state drug-testing for welfare laws carry on these American legacies. In Chapter 1 I examine how the classist, sexist, and …

Quantification Of Bacterial Shedding From The Respiratory Tract Of Health Care Workers Wearing Paprs And Other Types Of Air-Purifying Respirators On Sterile Conditions In A Simulated Operating Rooms (Ors), Segun Olanrewaju Ajewole

Graduate Theses, Dissertations, and Problem Reports (ETD)

The role of powered air- purifying respirators (PAPRs) in healthcare settings during infectious diseases outbreaks and highly contagious pathogens (e.g., SARS, HINI, Ebola, COVID etc.) has attracted much interest based on the level of respiratory protection they provide and their many positive user features. A common practice among healthcare workers to minimize airborne contaminant exposure has been to use surgical masks or N95 filtering facepiece respirators (FFRs). Surgical masks have been shown to offer minimal respiratory protection and while N95 FFRs offer better respiratory protection, they are not comfortable to use for a prolonged period. With current PAPR designs, exhaled …

Analyzing And Measuring Petrophysical Properties Of Marcellus Shale, Yahia Mustafa Yassin Mustafa

Graduate Theses, Dissertations, and Problem Reports (ETD)

Unconventional oil and natural gas play a key role in our nation's future. The U.S. has vast reserves of such resources that are commercially viable as a result of advances in horizontal drilling and hydraulic fracturing technology. These technologies enable greater access to oil and natural gas in shale formations. Responsible development of America's shale gas resources offers important economic, energy security, and environmental benefits. Hydraulic fracturing and horizontal drilling apply the latest technologies and make it commercially viable to recover shale gas and oil.

In order to estimate the original gas-in-place, predict the production rates, and optimize the hydraulic …

A Survey Of Musical Expectations In The Marine Forces Reserve Band Stationed In New Orleans, Louisiana, Brandon Paul Carbonari

Graduate Theses, Dissertations, and Problem Reports (ETD)

This research document examines the musical expectations of United States Marines serving in the Marine Forces Reserve Band from 2017 to 2021. Chapter One of this study provides a detailed foundation of the musical and non-musical responsibilities of a United States Marine musician. Training regime, rehearsal schedule, and logistical area responsibilities will be described to help convey the full scope of duty. The source material for Chapter One includes dissertations, journal articles, published books, online sources, and interviews. Chapter Two provides results of a survey that was conducted with members of the band. The questions were designed to record the …

Pseudomonas Aeruginosa Vaccine Development Using Dual-Species Whole Cell And Subunit Vaccines, Catherine Bell Blackwood

Graduate Theses, Dissertations, and Problem Reports (ETD)

Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen which can cause severe, recurrent, and chronic infections. The pathogen is highly adaptable, and pneumonia caused by it is associated with high morbidity and mortality, especially in individuals with the genetic disorder cystic fibrosis. Antibiotic resistance amongst clinical isolates of P. aeruginosa is steadily increasing, and multi-drug resistant strains are prevalent. There is currently no vaccine available for commercial use against P. aeruginosa. For these reasons, we sought to understand the immunity to P. aeruginosa induced by whole cell vaccination and identify antigens for development of future subunit vaccines. In this dissertation, …

Examining Changes In Learning And Engagement Of Higher Education Students In A Fully Online Flipped Learning Distance Education Classroom, James David Riel

Graduate Theses, Dissertations, and Problem Reports (ETD)

The challenge of implementing effective online distance education courses for academics and institutions is a centuries-old task. We can look across early developments in the 18^th century with the creation and delivery of correspondence courses; into the 20^th century with teaching and learning across analog methods such as audio and video; and now in the current era of digitized mechanisms that enable the online classroom. This includes advances in internet technologies and computing abilities that are the empowering the backbone processes, bridging connectivity between the student and the instructor. As society has trended toward massive increases in online …

Mitochondrial Aspects Of Neuronal Pathology In Triple-Transgenic Alzheimer’S Disease Mice, John Zachary Cavendish

Graduate Theses, Dissertations, and Problem Reports (ETD)

Alzheimer’s disease (AD) is a fatal, progressive neurodegenerative disease afflicting millions of people in the United States alone and is the only one of the top leading causes of morbidity and mortality with no effective disease-modifying therapies. It is the most common form of dementia, affecting one in three people over the age of 85. While the hallmarks of the disease include accumulation of beta-amyloid-based extracellular plaques and hyperphosphorylated tau-based intracellular neurofibrillary tangles, treatment strategies centered on removing or mitigating these components of AD have all failed in humans. Mitochondrial dysfunction has been increasingly recognized as an early and consistent …

Three Essays On The Impact Of Cuteness On Consumer Behavior, Alexis Taeeun Yim

Graduate Theses, Dissertations, and Problem Reports (ETD)

Over the last decade, the world has seen a rise in the popularity of cute stimuli. Adorable baby pictures, fluffy puppy videos, and whimsical emojis seem ever-present in social media news feeds and friends’ posts. In addition, products marketed toward adults that feature cute characters drive sales worth billions of dollars. The growing presence of cute stimuli in our daily lives is accompanied by emerging research on their social and behavioral impacts. Correspondingly, the current dissertation consists of three essays that contribute to the literature on cuteness by empirically testing the impacts of cuteness in the marketplace. To extend the …

A Deeper Inquiry Into The Transitional Needs Of Student Veterans, Nathan Calvin Bridendolph Jr

Graduate Theses, Dissertations, and Problem Reports (ETD)

Previous research revealed that veterans transitioning to post-secondary institutions have unique characteristics (PNPI, 2018; Wurster et al., 2013) and may suffer from a constellation of physical, psychological, and social problems that can impact their ability to perform to their full potential on campus (Ackerman et al., 2009; Alschuler & Yarab, 2016; Igielnik, 2019). Despite knowing the struggles student veterans (SVs) face when transitioning to campus, no studies were found that directly gain SVs’ input on what they believe will help their transition. The current study sought to gain a holistic perspective of SV needs by using the biopsychosocial model and …

Perceptions And Instructional Practices Used By West Virginian Secondary Agricultural Teachers In Meat Science Education, Kindra D. Carr

Graduate Theses, Dissertations, and Problem Reports (ETD)

A focus on classroom integration of science, technology, engineering, and math (STEM) concepts has resulted in secondary teachers broadening the scope and delivery of STEM principles in their classes. Because agriculture classes allow students to convert abstract ideas into concrete ideas, they provide a useful setting to teach STEM concepts. Using secondary agricultural classes to teach science is effective and has contributed to higher standardized science test scores, as opposed to a science class alone. West Virginia is known for meat processing facilities and meat technology learning activities in its public schools. However, little is known about how the content …

Covid-19 Vaccine Rollout: Examining Covid-19 Vaccination Perceptions And Intention Among Nurses, Emilee T. Austin

Graduate Theses, Dissertations, and Problem Reports (ETD)

Nurses’ COVID-19 vaccination rates have been reportedly low for being among the first prioritized for vaccination. To understand and potentially explain uptake barriers, this thesis utilized the 5c Model, the Integrative Model, the Extended Parallel Process Model, Uncertainty Management Theory, and the Theory of Motivated Information Management. This project used an online survey with a convenience sample recruited through the WV Nurses Association. Specifically, there were 328 nurses recruited, then screened for fully vaccinated participants leaving an analytic sample of 174 West Virginia nurses who had not yet been vaccinated. Participants were asked about their nursing role, threat perceptions, susceptibility …

Using Zebrafish To Elucidate The Expression And Gene Regulatory Network Of The Genomic Screen Homeobox Transcription Factors And Bring Innovative Science Learning Experiences To West Virginia, Rebecca Ann Coltogirone

Graduate Theses, Dissertations, and Problem Reports (ETD)

Central nervous system (CNS) development requires a code of regionally expressed transcription factors that impart initial neuronal cell identity, connectivity, and function. The absence of a transcription factor code would eliminate the neurodevelopmental logic producing diverse cell types in the CNS. Furthermore, early disruptions in transcription factor expression can affect later connectivity and function of neuronal circuits mediating sensory processing, defects in which are often observed as comorbid with various neurodevelopmental disorders (NDDs). Characterizing transcription factor expression and function is therefore an essential step in discerning the molecular mechanisms underlying human NDDs. genomic screen homeobox 1 and 2 (gsx1 …

Nitrite Supplementation Restores Aortic Health In Chronically Stressed Mice, Tyler Coblentz

Graduate Theses, Dissertations, and Problem Reports (ETD)

Chronic stress increases the risk of developing cardiovascular disease potentially via increases in elastic artery stiffness and endothelial dysfunction. Decreased bioavailability of the potent vasodilator nitric oxide (NO) describes a hallmark of endothelial dysfunction. Nitrite reduces into active NO, providing an alternative pathway of NO restoration. The effect of nitrite supplementation on aortic endothelial function has yet to be determined in chronically stressed mice. We hypothesized that nitrite supplementation would rescue chronic stress-attributed impairments in aortic endothelial function.

Mice at 18 weeks of age underwent an unpredictable chronic mild stress (UCMS; 5 days/week for 7 hrs/day) paradigm to elicit a …

Getting Connected: Exploring The Role Of Sustainability Labels In Tourism Marketing Communications, Elvira Kizilova

Graduate Theses, Dissertations, and Problem Reports (ETD)

While sustainability has become an important brand differentiator in many industries, current research suggests that tourism businesses rarely use sustainability labels in their communications, e.g., on their websites or other types of advertisements.

This dissertation examines the role of sustainability labels in increasing consumer preferences for sustainable travel destinations. Drawing on social exchange theory and anthropomorphism framework, we propose that sustainability labels increase consumer visit intentions, willingness to recommend a destination to someone else, and willingness to pay price premium for the sustainable offer. These effects are mediated by perception of goal congruence and connectedness with a destination. We further …

Sexual And Place-Based Identity: A Life Course Analysis Of Lgbtq+ Undergraduate Understandings Of Climate Change In Appalachia, Brandon Anthony Rothrock

Graduate Theses, Dissertations, and Problem Reports (ETD)

LGBTQ+ persons face heightened vulnerability to climate change-induced disasters due to their sexual orientation, gender identity, and gender expression. Yet, there are limited studies that examine how LGBTQ+ students, particularly in a higher-education setting, understand climate change in relation to their sexuality. With a majority of studies on LGBTQ+ persons and climate change focusing on LGBTQ+ experience during and after disaster, there is a gap in understanding LGBTQ+ perceptions of climate change in the day-to-day. Particularly in Appalachia, a region characterized by a strong place-attachment to and a collective identity with the natural environment, studies of marginalized groups’ perceptions of …

Development Of Synthetic Coal Char Simulant For Microwave Conversion Studies: A Computationally-Driven Approach, Kevin A. Hager

Graduate Theses, Dissertations, and Problem Reports (ETD)

Recent experimental demonstration of new reaction windows for coal char/methane reactions that are less energy-intensive, provides innovation for modular reactors. However, the correlation of the exact mechanism for the enhancement of these reaction windows is not certain. This study investigates the simplification of these experimental studies by developing a well-characterized coal char simulant. The approach involves using a computational approach to screen macroscopic composition to replicate the dielectric and compositional response of actual char. This study is focused on PRB coal char. A discrete element method (DEM) technique was used to simulate the packing of coal chars to give the …

Re-Design Of Precision Signal Conditioning Circuit For Detecting Schumann Resonance, Rohith Bikkina

Graduate Theses, Dissertations, and Problem Reports (ETD)

Extremely low frequencies signals are waves between 3 to 30Hz and corresponding wavelengths between 10,000 to 100,000 kilometers. The specific signals used here are generated from lightning and are excited at frequencies around 8Hz, 14Hz, 20Hz. These are often called Schumann Resonance frequencies. Several stations have been built around the world for identifying ELF waves. All of those required a sparsely populated area that was far away from electric power lines because of interference from electric noise at 50 Hz and 60Hz. This project develops and tests an amplifier and filter circuit that should assist in identifying the Schumann Resonance …

Geology Oriented Loading Approach For Underground Coal Mines, Deniz Tuncay

Graduate Theses, Dissertations, and Problem Reports (ETD)

Mining-induced stresses in underground coal mines play a significant role in pillar and support design, hence in the safety of mining operations. Adequate design of pillars and roof control plans rely on the accurate assessment of the mining-induced loads, as well as the load-bearing capacities of the supports. In pillar design tools frequently used by the underground coal mining industry and Mine Safety and Health Administration (MSHA), overburden loading is estimated by geometric concepts such as the tributary area and the abutment angle method. These methods do not explicitly consider important mechanical responses such as specific overall ground behavior. The …

Hearing Ourselves Speak: Finding The Trans Sound In The Ohio River Valley, Gwendolyn Patricia Saporito-Emler

Graduate Theses, Dissertations, and Problem Reports (ETD)

This thesis discusses at length the experiences of four interviewees, selected for being both musicians as well as transgender people. From the author’s shared perspective as a trans woman, this work addresses the issues and boons of being trans musicians. It reflects their experiences, both positive and negative, as well as provides conjectural analyses of the respondents’ shared stories. It identifies common themes, issues regularly experienced by trans people, and offers arguments on why ending this hate is so vitally essential.

Improving Microbial Safety Of Locally Grown Produce In West Virginia Area, Kawang Li

Graduate Theses, Dissertations, and Problem Reports (ETD)

There is a growing concern regarding the microbial safety of farmers’ markets as fresh produce consumption in the United States is gaining popularity. The CDC reported 46% of foodborne illnesses are related to fresh produce. Consuming fresh produce raw removes a processing step, increasing the risk of cross-contamination, leading to illness. However, this risk can be mitigated by washing produce with antimicrobial solutions during post-harvest processes. To reduce the microbial load on fresh produce, the triple-wash process, where the produce is rinsed with water twice and lastly an antimicrobial solution (WWA). WWA is recommended by the WVU Extension Service Small …