Open Access. Powered by Scholars. Published by Universities.®

Digital Commons Network™

Open Access. Powered by Scholars. Published by Universities.®

Graduate Theses, Dissertations, and Problem Reports (ETD)

Discipline
Keyword
Publication Year
File Type

Articles 2131 - 2160 of 2809

Full-Text Articles in Entire DC Network

Mass Spectrometry Of Metallothionein Adducts As Candidate Biomarkers Of Styrene Oxide And 1-Phenylpropylene Oxide, Sandra G. Tarr Jan 2005

Mass Spectrometry Of Metallothionein Adducts As Candidate Biomarkers Of Styrene Oxide And 1-Phenylpropylene Oxide, Sandra G. Tarr

Graduate Theses, Dissertations, and Problem Reports (ETD)

Metallothioneins (MT) are cysteine-rich intracellular proteins that sequester epoxides, such as the genotoxic styrene metabolite, styrene oxide (SO). Covalent adduct formation by the ring-opening of SO and its homologue, 1-phenylpropylene oxide (trans), with MT forms stable complexes that potentially may serve as biomarkers for epoxide exposure. MT was reacted with SO and trans-phenylpropylene oxide (PPO) separately in water. Mass spectra of adducts were obtained using electrospray ionization with positive-ion detection in an ion trap mass spectrometer. MS/MS analysis established the covalent nature of the complexation. Further experiments were conducted to determine the site of adduct formation. Molecular modeling was used …

Go to article

Functional Analysis Of Atrpd3b, A Rpd3-Type Histone Deacetylase, In Arabidopsis, Lin Zhang Jan 2005

Functional Analysis Of Atrpd3b, A Rpd3-Type Histone Deacetylase, In Arabidopsis, Lin Zhang

Graduate Theses, Dissertations, and Problem Reports (ETD)

Histone acetylation is modulated through the action of histone acetyltransferases and deacetylases, which play key roles in the regulation of eukaryotic gene expression. The RPD3 group of histone deacetylases constitutes the first class of histone deacetylases (HDACs) in eukaryotes.;In yeast and mammalian cells, it was found that RPD3 histone deacetylases are involved in the ageing and development. In contrast much less is known about the function of RPD3s in plant. Here I studied AtRPD3B, a RPD3-type histone deacetylase in Arabidopsis thaliana, to define its role in plant development and signal transduction pathways. Using the AtRPD3B: GUS transgenic Arabidopsis plants, I …

Go to article

Remote Sensing Of Corridor Landscapes: A Case Study Of The National Road, Wheeling, West Virginia., Brian N. Renzella Jan 2005

Remote Sensing Of Corridor Landscapes: A Case Study Of The National Road, Wheeling, West Virginia., Brian N. Renzella

Graduate Theses, Dissertations, and Problem Reports (ETD)

This research is an investigation of how remote sensing can be used to supplement traditional methods of corridor management planning. Cultural resource inventory for corridor and interpretive management practices is an important component of applications for the designation of a selected route as Scenic Historic American Byways through the National Scenic Byways Program. A case study of a section of the National Road near Wheeling, West Virginia, showed that contemporary and historic aerial photography can facilitate the identification of possible impacts on a route’s historic integrity. A previously designated historic route, the National Road was America’s first federally funded interstate …

Go to article

The Monyton Diaspora: A History Of The Middle Ohio River Valley, 1640-1700., Isaac J. Emrick Jan 2005

The Monyton Diaspora: A History Of The Middle Ohio River Valley, 1640-1700., Isaac J. Emrick

Graduate Theses, Dissertations, and Problem Reports (ETD)

During the seventeenth century a vibrant group of Native Americans, the Monytons, controlled southern West Virginia and northeastern Kentucky. The effects of contact with Europeans destabilized their societies as far inland as the Ohio Valley. This began the process which eventually pushed most Monytons from the region. The Five Nations Iroquois, pressured by social changes farther north, attacked the Monytons further drawing people out of the Ohio Valley. A growing southern Indian slave trade also contributed to the decay of Monyton dominance in the region. A central point in this thesis is that Monytons formed and reformed traditions to deal …

Go to article

Aterf4 And Aterf7 Are Involved In The Abscisic Acid Signaling Response In Arabidopsis., Zhen Yang Jan 2005

Aterf4 And Aterf7 Are Involved In The Abscisic Acid Signaling Response In Arabidopsis., Zhen Yang

Graduate Theses, Dissertations, and Problem Reports (ETD)

ERFs (ethylene-responsive element binding factors) belong to a large family of transcription factors that are found exclusively in plants. A small subfamily of ERF proteins can act as transcriptional repressors. The Arabidopsis genome contains eight ERF repressors, namely AtERF3, AtERF4, AtERF7, AtERF8, AtERF9, AtERF10, AtERF11, and AtERF12. The different ERF repressors show differential expression, suggesting that they may have different function. The expression of AtERF4 can be induced by ethylene, jasmonic acid, or abscisic acid. AtERF7 can be induced by abscisic acid only. Using a transient expression system, we demonstrated that AtERF4 and AtERF7 can function as transcriptional repressors. By …

Go to article

The Emergence And Decline Of The Delaware Indian Nation In Western Pennsylvania And The Ohio Country, 1730--1795, Richard S. Grimes Jan 2005

The Emergence And Decline Of The Delaware Indian Nation In Western Pennsylvania And The Ohio Country, 1730--1795, Richard S. Grimes

Graduate Theses, Dissertations, and Problem Reports (ETD)

Many past and current generations of historians, anthropologists, and literary writers have acknowledged the existence of a Delaware Indian nation. They, however, have failed to thoroughly understand or address the historical and cultural dynamics that contributed to both the formation and quick decline of this Indian nation. This multidisciplinary study includes the oral traditions and oratory of Delaware Indians, the observances of Moravian missionaries and colonial-revolutionary officials, and contemporary anthropological and historical sources, to construct the building of the Delaware nation during the eighteenth century.;Once decentralized and living in the Delaware River watershed, three phratries or animal tribes (Turtle, Turkey, …

Go to article

Role Of The Host Cell In The Type Iii Translocation Of Pseudomonas Aeruginosa Exoenzyme S, Elizabeth A. Rucks Jan 2005

Role Of The Host Cell In The Type Iii Translocation Of Pseudomonas Aeruginosa Exoenzyme S, Elizabeth A. Rucks

Graduate Theses, Dissertations, and Problem Reports (ETD)

Exoenzyme S (ExoS) is a bifunctional toxin that is directly translocated into eukaryotic cells by the Pseudomonas aeruginosa (Pa) type III secretory (T3S) process. The N-terminal region of ExoS includes a GTPase activating protein (GAP) activity and the C-terminal region includes an ADP-ribosyltransferase (ADPRT) activity. The T3S-translocation (T3S-T) of ExoS allows the bacteria to directly manipulate host cell function, thereby contributing the virulence of Pa. A cell culture model of infection was developed to study the effects of ExoS on cell function, which differentiates the effects of ExoS expression from other bacterial effects. Previous studies found that ExoS inactivates cell …

Go to article

Determination Of Rx Expression In The Adult Mouse Retina And Delineation Of The Rx Mediated Gene Regulation, Supriya A. Shah Jan 2005

Determination Of Rx Expression In The Adult Mouse Retina And Delineation Of The Rx Mediated Gene Regulation, Supriya A. Shah

Graduate Theses, Dissertations, and Problem Reports (ETD)

The Retinal homeobox gene, Rx, is expressed in the anterior neural plate and the optic vesicle shows Rx expression throughout development. Rx is required for the proliferation of retinal progenitor cells during eye development.;Our expression studies of the Rx gene in the adult mouse retina show Rx expression in the Photoreceptor and inner nuclear layer of the adult neural retina and ciliarybody. The cotransfection assays indicate that Rx supports the transcription of neural retinal markers, as well as the RPE-markers. Therefore we characterize Rx as a proliferation marker for the entire optic vesicle region.;To determine the mechanism of Rx-mediated regulation …

Go to article

Fourth Graders' Development Of Multimedia Portfolios., Veronica D. Kuminkoski-Yurt Jan 2005

Fourth Graders' Development Of Multimedia Portfolios., Veronica D. Kuminkoski-Yurt

Graduate Theses, Dissertations, and Problem Reports (ETD)

A review of the literature indicates there is a need for alternative assessment practices for elementary students. Seven fourth grade students in a small rural school will learn how to construct an electronic portfolio that will exhibit their progress and achievement. The contents of the portfolio must include student participation in the selection as well as provide evidence of self-reflection. This study researches a computer-based portfolio, which uses HyperStudio software. This program will allow students to create sound, video, graphics, and animation during the construction process. As students build their multimedia portfolios, they will learn how to use technology as …

Go to article

The Role Of Apoptosis In Muscle Remodeling, Parco Ming-Fai Siu Jan 2005

The Role Of Apoptosis In Muscle Remodeling, Parco Ming-Fai Siu

Graduate Theses, Dissertations, and Problem Reports (ETD)

Apoptosis has been implicated in mediating the process of muscle loss during muscle disuse and with aging. However, the physiologic role of apoptosis in muscle remodeling is largely unknown. The purposes of this dissertation were to examine the role of apoptosis in different muscle remodeling conditions including muscle denervation, hindlimb suspension, muscle unloading following overload, endurance treadmill training, and muscle overload in mice, rats, or quails. Apoptotic signaling components and cellular stress markers including Bax, Bcl-2, Apaf-1, cytochrome c, caspases, Smac/DIABLO, XIAP, ARC, FLIP, AIF, p53, Id2, p21, c-Myc, PARP, HSP70, HSP27, HSP60, MnSOD, CuZnSOD, catalase, H2O2, MDA/4-HAE, nitrotyrosine or …

Go to article

Investigation Of The Effects Of Thermal Enrichment And Acid Mine Drainage On Sensitive Aquatic Biota In The Stony River, Grant County, West Virginia., Christopher D. Horn Jan 2005

Investigation Of The Effects Of Thermal Enrichment And Acid Mine Drainage On Sensitive Aquatic Biota In The Stony River, Grant County, West Virginia., Christopher D. Horn

Graduate Theses, Dissertations, and Problem Reports (ETD)

Recent surveys have indicated that populations of fishes and crayfish are small in the Stony River, Grant Co., WV. The Stony River is influenced by thermal enrichment and acid mine drainage (AMD) within a 13 km stream reach, downstream of Mt. Storm Lake. In an attempt to determine the influence of each anthropogenic disturbance on these affected taxa, studies of their distribution and survival were undertaken. A crayfish trapping survey was conducted along a gradient of thermal and AMD influences in the Stony River system. Cambarus bartonii was the only species captured, although two subspecies of C. bartonii living in …

Go to article

The Hospitalized Adolescent: Fears, Stressors, And Coping Skills., Lora L. Garlitz Jan 2005

The Hospitalized Adolescent: Fears, Stressors, And Coping Skills., Lora L. Garlitz

Graduate Theses, Dissertations, and Problem Reports (ETD)

This paper presents the findings of an investigation to determine whether of not there is a difference in hospitalized adolescents and non-hospitalized adolescents. The participants were 60 adolescents between the ages of 12 to 18 years. Their fears, stressors, and coping skills were evaluated by using three instruments: a 35-item self-report test on coping; a 108-item self-report test on fears; and a 15-item self-report test on stress. The results revealed that on specific measures hospitalized adolescents exhibited a statistically significantly less degree of emotional upset during hospitalization than did the community sample. Implications of these results in determining procedures to …

Go to article

I. Development Of The West Virginia Dairy Quality Assurance Program, Daniel H. Poole Jan 2005

I. Development Of The West Virginia Dairy Quality Assurance Program, Daniel H. Poole

Graduate Theses, Dissertations, and Problem Reports (ETD)

Consumer confidence impacts agricultural markets. In response, many food industries have adopted cost-effective programs to reduce risk and offer third party verification of product quality. The purpose of this project was to implement the West Virginia Dairy Quality Assurance Program (WVDQAP) which promotes best management practices to produce high quality milk and meat products. This study surveyed dairy producers throughout the state, to establish the most effective method of program implementation. A manual was constructed and presented to producers at one of five meeting across the state. The manual focuses on the requirements a producer must follow to receive certification. …

Go to article

Art Interpretation: Constructing Meaning Through Poetic And Non-Verbal Responses., Lori A. Mcpherson Jan 2005

Art Interpretation: Constructing Meaning Through Poetic And Non-Verbal Responses., Lori A. Mcpherson

Graduate Theses, Dissertations, and Problem Reports (ETD)

Critical inquiry and personal response models with regard to art interpretation are composed of many forms of activities and response exercises that are both poetic and non-verbal. Students have been responding to art using critical inquiry, but there has been relatively nothing written in regard to poetic expression and non-verbal responding in the teaching of art interpretation. This project will discuss how a poetic model (the use of figurative language to form ideas about constructing personal meaning in a work of art) and a non-verbal model (creating a visual response to exemplify meaning) work together in the construction of meaning. …

Go to article

Prostaglandin F(2alpha) (Pgf(2alpha))-Independent And -Dependent Regulation Of The Bovine Luteal Endothelin System., Ekta Choudhary Jan 2005

Prostaglandin F(2alpha) (Pgf(2alpha))-Independent And -Dependent Regulation Of The Bovine Luteal Endothelin System., Ekta Choudhary

Graduate Theses, Dissertations, and Problem Reports (ETD)

The genes of the endothelin system are the potential targets for regulation by prostaglandin F2α (PGF2α) and have been studied in greater detail. The effects of a luteolytic dose of PGF2α on the mRNA encoding endothelin converting enzyme-1 (ECE1), pre-pro endothelin-1 (ET-1) and the ET receptors ETA, ETB, and in bovine corpus luteum (CL) collected during the early, mid or late luteal phases were examined. The effects of treatment with PGF2α on ECE-1 protein, big ET-1 and the biologically active mature ET-1 were examined. Most importantly, the direct activity of ECE-1 was determined. Based on the results it is suggested …

Go to article

Management Of The Suckled Post-Partum Beef Cows: Pasture-Based Weaning System., John F. Odhiambo Jan 2005

Management Of The Suckled Post-Partum Beef Cows: Pasture-Based Weaning System., John F. Odhiambo

Graduate Theses, Dissertations, and Problem Reports (ETD)

Low grain prices and recurrent periods of drought have elicited interest in early weaning. Calves can efficiently utilize feed for weight gain when it is fed directly, rather than through their dams. This study evaluated calf response in three weaning systems (experiment 1) and profiles of body energy reserves in early-weaned beef cows (experiment 2). Treatment x year, and calf sex x year interaction (P < 0.05) were observed for calf weight gain. These interactions underscored the need for constant readjustments in management systems that incorporate early weaning in their programs. In experiment 2, body weight and condition score of cows were affected marginally (P = 0.06) by early weaning, whereas ribfat and rumpfat differed (P < 0.05) between earlyweaned and control cows. These findings indicated that subjective methods (body weight and condition score) should be used in combination with more objective measurements (ribfat and/or rumpfat) for accurate evaluation of energy status of beef cows. Cows of various ages responded differently to early weaning. Three to five-year-old cows exhibited greater response to early weaning as opposed to 2-year-olds and cows > 5 years of age, indicating that profit potential of the cow-calf operation can be improved by altered management of middle-aged cows.

Go to article

The Effects Of Roads And Culverts On Stream And Stream-Side Salamander Communities In Eastern West Virginia., Ryan Lee Ward Jan 2005

The Effects Of Roads And Culverts On Stream And Stream-Side Salamander Communities In Eastern West Virginia., Ryan Lee Ward

Graduate Theses, Dissertations, and Problem Reports (ETD)

Roads and culverts have many effects on stream salamanders. We examined 120 culvert crossings in the Shavers Fork and Dry Fork watersheds in eastern West Virginia. Complete barriers to salamander passage occurred at 55.0% of the sites visited and partial barriers at 34.2%. Analyses showed that 20.6% of the total stream length in the Dry Fork watershed and 18.4% in the Shavers Fork watershed were isolated by barriers. We conducted salamander sampling at 16 streams and used Akaikeís Information Criterion to select the best a priori models explaining salamander diversity, richness, and abundance. Roads benefited disturbance tolerant species while negatively …

Go to article

The Effects Of Uv-B Radiation On The Growth And Physiology Of Non-Agricultural Plant Species Under Changing Environmental Conditions., Chintamani S. Manish Jan 2005

The Effects Of Uv-B Radiation On The Growth And Physiology Of Non-Agricultural Plant Species Under Changing Environmental Conditions., Chintamani S. Manish

Graduate Theses, Dissertations, and Problem Reports (ETD)

I investigated whether seedlings of non-agricultural plants exposed to varying levels of UV-B exposure had altered growth, physiological, and tissue chemistry responses under changing environmental conditions. In a field experiment, using a modulated UV-B lamp bank system, elevated UV-B did not significantly alter the total biomass of the 6 plant species that were studied, and only Quercus rubra showed a trend towards some sensitivity to UV-B. In two species—Platanus occidentalis and Setaria faberii—I found an increase in the production of UV-B absorbing compounds under elevated UV-B while two grasses—S. faberii and Elymus virginicus—had significantly decreased C:N ratios under elevated UV-B. …

Go to article

The Effects Of The Route Of Viral Infection On The Balance Of T Helper Immune Responses., Alicia R. Mathers Jan 2005

The Effects Of The Route Of Viral Infection On The Balance Of T Helper Immune Responses., Alicia R. Mathers

Graduate Theses, Dissertations, and Problem Reports (ETD)

The majority of pathogens enter the host through mucosal surfaces. Therefore, it stands to reason that production of protective mucosal immunity is a desirable outcome following vaccination for those infections. However, only a few mucosal vaccines have been developed to date, in part because of a general lack of understanding of mucosal immunity. The research presented in this dissertation is focused on the comparison of mucosal and systemic immunizations as a means of contributing to the understanding of how mucosal immune responses are induced. There is evidence that mucosal immunization can skew the balance of T helper (Th) 1 and …

Go to article

Cytochrome P450 1a-Substrate Interactions: Determinants Of Substrate Selectivity And Stereo- And Regiospecificity Of Oxidation., Spencer Snyder Ericksen Jan 2005

Cytochrome P450 1a-Substrate Interactions: Determinants Of Substrate Selectivity And Stereo- And Regiospecificity Of Oxidation., Spencer Snyder Ericksen

Graduate Theses, Dissertations, and Problem Reports (ETD)

Cytochromes P450 comprise a ubiquitous superfamily of heme-thiolate enzymes, considered among the most versatile biological catalysts known. Structure-function relationships of P450s are of particular interest to those attempting to elucidate such aspects of xenobiotic metabolism as substrate selectivity and regiospecific oxidations. We studied two members of the P450 1A subfamily, P450 1A1 and 1A2, found in highest abundance in lung and liver tissues, respectively. These enzymes may have an important link to cancer, as both are known to regiospecifically activate procarcinogenic substrates. To gain a better understanding of structure-function relationships in these enzymes, we applied a combination of site-directed mutagenesis, …

Go to article

The Role Of The Rx Homeobox Gene In Development Of The Eye And Pituitary Gland., Elena A. Kozhemyakina Jan 2005

The Role Of The Rx Homeobox Gene In Development Of The Eye And Pituitary Gland., Elena A. Kozhemyakina

Graduate Theses, Dissertations, and Problem Reports (ETD)

Rx is a paired-like homeodomain transcription factor that is expressed in the developing eyes, ventral forebrain, hypothalamus, posterior pituitary, and the pineal gland. In adults, Rx is primarily expressed in the neural retina, ciliary bodies, pineal gland, hypothalamus, and posterior pituitary. Rx mutations in multiple species are associated with anophthalmia. During the course if this work, we identified a human patient with anophthalmia and sclerocornea that carries mutations in the RX gene. One allele of the patient carries a Q147X mutation, which causes a truncation of the RX protein at the beginning of the homeodomain. The other allele possesses an …

Go to article

Exposures To Silica And Inducers Of Xenobiotic Metabolism In The Rat Lung, Lori A. Battelli Dec 2004

Exposures To Silica And Inducers Of Xenobiotic Metabolism In The Rat Lung, Lori A. Battelli

Graduate Theses, Dissertations, and Problem Reports (ETD)

CYP1A1 metabolizes polycyclic aromatic hydrocarbons (PAHs), such as those in cigarettes, to reactive intermediates which interact with DNA and lead to cancer. Silica is a lung carcinogen. Epidemiology studies of silica and lung cancer are not all positive. A possible explanation for these inconsistencies may be that silica is a modifier of PAH metabolism and, thus, cigarette smoke carcinogenesis. We hypothesize that crystalline silica exposure alters CYP1A1 expression, thereby modifying lung cancer risk. Rats exposed to both crystalline silica and the model PAH, beta-naphthoflavone (NF), a CYP1A1 inducer have significantly decreased CYP1A1 enzymatic activity and CYP1A1 protein expression. In the …

Go to article

Finite Element Analysis Of Interaction Between Actin Cytoskeleton And Intracellular Fluid In Prechondrocytes And Chondrocytes Subjected To Compressive Loading, Sundar Viswanathan Dec 2004

Finite Element Analysis Of Interaction Between Actin Cytoskeleton And Intracellular Fluid In Prechondrocytes And Chondrocytes Subjected To Compressive Loading, Sundar Viswanathan

Graduate Theses, Dissertations, and Problem Reports (ETD)

Prechondrocytes exhibit a change in their actin cytoskeletal arrangement when subjected to loading. This study looks at the implications of the altered solid-fluid interactions due to this change. A 2-D Finite element model of a cell was developed. A solid-fluid interaction solution procedure was validated and used to determine fluid flow/pressure in a cell when subject to deformation. These values were then used to calculate fluid flow induced stresses on individual actin fibers. The results suggest that prechondrocytes alter their cytoskeletal structure to reduce stresses as a result of fluid flow due to deformation of the cell.

Go to article

Effectiveness Of Electrical Demand Reduction Strategies, Chandra Gopalakrishnan Dec 2004

Effectiveness Of Electrical Demand Reduction Strategies, Chandra Gopalakrishnan

Graduate Theses, Dissertations, and Problem Reports (ETD)

A common notion in the industry is that energy is an overhead and not related directly to the product. Measures that reduce electric demand are usually considered in isolation and their effect on the other costs related to the product is ignored. This is especially true for multi-product operations where the demand for various products is not constant and it is hard to track the costs associated with each product. This research takes a software based approach to analyze how a demand reduction measure affects the carrying cost, stockout cost, labor cost, electric usage cost and electric demand cost. This …

Go to article

Effects Of Herbivory By White -Tailed Deer (Odocoileus Virginianus Zimm.) On The Population Ecology And Conservation Biology Of American Ginseng (Panax Quinquefolius L.), Mary Ann Furedi Dec 2004

Effects Of Herbivory By White -Tailed Deer (Odocoileus Virginianus Zimm.) On The Population Ecology And Conservation Biology Of American Ginseng (Panax Quinquefolius L.), Mary Ann Furedi

Graduate Theses, Dissertations, and Problem Reports (ETD)

American ginseng (Panax quinquefolius L.) is a long-lived understory herb considered rare within its range. Harvesting pressures and habitat degradation/destruction are thought to be the primary causes of population decline although others factors such as browsing by white-tailed deer (Odocoileus virginianus Zimm.) have not yet been explored. To understand the effects of deer browsing on natural ginseng populations, my research focused on four specific objectives. First, I investigated how plant characteristics and microsite conditions influence the susceptibility to being browsed. I found that apparency plays a role in influencing browse selection, such that large, reproductive plants in visible and accessible …

Go to article

What Is The Impact Of Parental Support On Early Literacy?, Deborah K. Bush Dec 2004

What Is The Impact Of Parental Support On Early Literacy?, Deborah K. Bush

Graduate Theses, Dissertations, and Problem Reports (ETD)

Today, more than 75 percent of women ages 25--34 and 75 percent of women with a child under six years of age participate in the labor force. When both parents work outside the home, they often want to send their child to preschool, but preschool classes aren't always available, and often those that are available are the government programs that are open only to those of lower socio-economic status.;In addition, parents often want to support the emergent literacy of their children and help prepare them for kindergarten but aren't sure of how to provide the type of assistance that might …

Go to article

Characterization Of Cyp2d Protein From Human Brain Cerebellum, Deepak Bhatia Dec 2004

Characterization Of Cyp2d Protein From Human Brain Cerebellum, Deepak Bhatia

Graduate Theses, Dissertations, and Problem Reports (ETD)

To date, knowledge in characterization of CYP proteins has been limited to immunoblotting, RT-PCR, immunohistochemistry and so forth; therefore, it is the intention of this study to investigate: (1) What kind of CYP2D protein is present in brain with modern proteomic tools; (2) Whether, CYP2D6 in brain is same as the liver CYP2D6; and (3) If CYP2D7 is present, can CYP2D6 polymorphism be found in the brain?;To answer these questions, this study used a one-step method of isolation of protein by immunoprecipitation followed by its identification using 2D-blots, nanospray LCMS, immunoblots and immunohistochemistry. Probing of immunoprecipitated proteins with polyclonal CYP2D6 …

Go to article

Potential Mechanisms Linking Nutrition And Neuroendocrine Control Of Reproduction In The Sheep, Adam Bradley Dobbins Dec 2004

Potential Mechanisms Linking Nutrition And Neuroendocrine Control Of Reproduction In The Sheep, Adam Bradley Dobbins

Graduate Theses, Dissertations, and Problem Reports (ETD)

Nutrition and reproduction are linked in that the nutritional status of an individual influences its reproductive performance, i.e. an individual in negative energy balance will have decreased ability to reproduce. The mechanisms in which nutrition influences reproduction are not understood. My data suggest that reductions in lutenizing hormone secretion resulting from photoperiod-induced increases in sensitivity to testosterone negative feedback may be mediated, at least in part, by elevated neuropeptide Y (NPY), a hypothalamic neuropeptide shown to alter gonadotropin releasing hormone release and potently affect food intake. The results from my second study support our hypothesis that the growth hormone mediated …

Go to article

Comparison Of Chestnut Canker Treatment Procedures For Hypovirus Introduction, Brian C. Bell Dec 2004

Comparison Of Chestnut Canker Treatment Procedures For Hypovirus Introduction, Brian C. Bell

Graduate Theses, Dissertations, and Problem Reports (ETD)

Historically, hypovirulent strains of Cryphonectria parasitica have been introduced into cankers principally by inoculating wounds made to the margin of cankers. Twelve hypovirus introduction methods were tested. Seventy-two trees were inoculated in June 2002 with an orange-pigmented virulent strain of C. parasitica (two inoculations per tree). After 11 weeks, the resulting cankers were treated with a brown-pigmented hypovirulent C. parasitica isolate using one of the following methods: (1) a non-invasive treatment where the inoculum was painted onto the canker surface; (2) an invasive treatment where the canker face was wounded with a sharp blade prior to the painting application of …

Go to article

Natural Gas Exploration Associated With Fracture Systems In Alleghenian Thrust Faults In The Greenbrier Formation, Southern West Virginia, Craig A. Edmonds Dec 2004

Natural Gas Exploration Associated With Fracture Systems In Alleghenian Thrust Faults In The Greenbrier Formation, Southern West Virginia, Craig A. Edmonds

Graduate Theses, Dissertations, and Problem Reports (ETD)

A hydrocarbon play has been identified in southern West Virginia targeting the intersection of thrust faults with specific Mississippian reservoirs. Late Paleozoic sandstone and limestone reservoirs are traditional pays of the study area, often yielding economic natural gas production, with initial production rates ranging between 100 to 200 Mcf per day per well. In contrast, production rates from wells that encounter faults often exceed 500 Mcf per day. We believe this prolific production results from secondary fracture porosity and enhanced permeability from fracture zones associated with thrust faults, providing a conduit to the Union Oolite reservoir member of the Greenbrier …

Go to article

First Prev Next Last